TPCN1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	TPCN1
Entrez Gene ID	567534
Full Name	two pore segment channel 1
Synonyms	si:dkey-125i10.5
General protein information	Preferred Names two pore segment channel 1 Names two pore calcium channel protein 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165219.4	XP_005165276.1	two pore calcium channel protein 1 isoform X2
XM_005165220.3	XP_005165277.1	two pore calcium channel protein 1 isoform X3
XM_690831.7	XP_695923.4	two pore calcium channel protein 1 isoform X1
XM_690831.8	XP_695923.4	two pore calcium channel protein 1 isoform X1
XM_005165220.2	XP_005165277.1	two pore calcium channel protein 1 isoform X3
XM_005165219.3	XP_005165276.1	two pore calcium channel protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TPCN1	XP_003313998.1
Canis lupus familiaris (dog)	TPCN1	XP_534690.2
Bos taurus (cattle)	TPCN1	XP_002694563.1
Gallus gallus (chicken)	TPCN1	XP_004934547.1
Homo sapiens (human)	TPCN1	NP_001137291.1
Rattus norvegicus (Norway rat)	Tpcn1	NP_647548.2
Danio rerio (zebrafish)	tpcn1	XP_005165277.1
Xenopus tropicalis (tropical clawed frog)	tpcn1	XP_002934333.2
	TPCN1	XP_002798827.1
Mus musculus (house mouse)	Tpcn1	NP_665852.1

Gene Ontology

The following TPCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TPCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa10361	XM_690831.8 Latest version!	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa10362	XM_005165219.4 Latest version!	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa10363	XM_005165220.3 Latest version!	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa10361	XM_690831.7	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa10363	XM_005165220.2	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa10362	XM_005165219.3	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TPCN1 ( XM_690831.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695923.4 Danio rerio two pore segment channel 1 (tpcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10361
Clone ID Related Accession (Same CDS sequence)	XM_690831.7 , XM_690831.8
Accession Version	XM_690831.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	two pore calcium channel protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_690831.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGCAGACAT CATTAGTGTT TCTGGGTCGA 
AGGTCACGTA CCAAGAGTGA CCTCAGTATG AAGATGTACG AAGAGGAGAT TCAGGAATGG 
TACGAGGAAT ATTCCAGAAC AAACCTTCAG ACTGATGGGA CCTTCCACAG AGAACTGGAC 
AGCCCTGATG CTGGTGCCAC AAACAGCATT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695923.4
CDS	1360..3795
Translation	MDISSTFDNMDTGLTQDRPVQMSSFYKEIEPVDGAGNHDVVNNVTIPASGASDPRAQNSLQQSWEMNFQEAAIYLQEGENNDKFFTHPRSAHALSAYLFAHSHLFYVVELLTAVLLMLLSLSEAPAVPFLRLDVYVHATLELLALVMVAFELCMKLRWLGFHTFIRHKRTMVKTSVLFIQFIEAIVVLVRQTSHLRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFAILGFCLFSANTADHYFSTLENSIVSLFVLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQRPDGVSLKQFDGLMRFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKGINLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIALAFDMEPFYFIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFETEVSQTEALQTLDFYKHTLGVTNLTSLQDMCVALERSGQTSLVFLGRRSRTKSDLSMKMYEEEIQEWYEEYSRTNLQTDGTFHRELDSPDAGATNSIN

Target ORF information:

RefSeq Version	XM_690831.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690831.7

ORF Insert Sequence:

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGCAGACAT CATTAGTGTT TCTGGGTCGA 
AGGTCACGTA CCAAGAGTGA CCTCAGTATG AAGATGTACG AAGAGGAGAT TCAGGAATGG 
TACGAGGAAT ATTCCAGAAC AAACCTTCAG ACTGATGGGA CCTTCCACAG AGAACTGGAC 
AGCCCTGATG CTGGTGCCAC AAACAGCATT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_690831.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695923.4 Danio rerio two pore segment channel 1 (tpcn1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10361
Clone ID Related Accession (Same CDS sequence)	XM_690831.7 , XM_690831.8
Accession Version	XM_690831.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2436bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_690831.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGCAGACAT CATTAGTGTT TCTGGGTCGA 
AGGTCACGTA CCAAGAGTGA CCTCAGTATG AAGATGTACG AAGAGGAGAT TCAGGAATGG 
TACGAGGAAT ATTCCAGAAC AAACCTTCAG ACTGATGGGA CCTTCCACAG AGAACTGGAC 
AGCCCTGATG CTGGTGCCAC AAACAGCATT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695923.4
CDS	1683..4118
Translation	MDISSTFDNMDTGLTQDRPVQMSSFYKEIEPVDGAGNHDVVNNVTIPASGASDPRAQNSLQQSWEMNFQEAAIYLQEGENNDKFFTHPRSAHALSAYLFAHSHLFYVVELLTAVLLMLLSLSEAPAVPFLRLDVYVHATLELLALVMVAFELCMKLRWLGFHTFIRHKRTMVKTSVLFIQFIEAIVVLVRQTSHLRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFAILGFCLFSANTADHYFSTLENSIVSLFVLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQRPDGVSLKQFDGLMRFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKGINLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIALAFDMEPFYFIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFETEVSQTEALQTLDFYKHTLGVTNLTSLQDMCVALERSGQTSLVFLGRRSRTKSDLSMKMYEEEIQEWYEEYSRTNLQTDGTFHRELDSPDAGATNSIN

Target ORF information:

RefSeq Version	XM_690831.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690831.8

ORF Insert Sequence:

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGCAGACAT CATTAGTGTT TCTGGGTCGA 
AGGTCACGTA CCAAGAGTGA CCTCAGTATG AAGATGTACG AAGAGGAGAT TCAGGAATGG 
TACGAGGAAT ATTCCAGAAC AAACCTTCAG ACTGATGGGA CCTTCCACAG AGAACTGGAC 
AGCCCTGATG CTGGTGCCAC AAACAGCATT AACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_005165219.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165276.1 Danio rerio two pore segment channel 1 (tpcn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10362
Clone ID Related Accession (Same CDS sequence)	XM_005165219.4 , XM_005165219.3
Accession Version	XM_005165219.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	two pore calcium channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165219.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGACATCAT TAGTGTTTCT GGGTCGAAGG 
TCACGTACCA AGAGTGACCT CAGTATGAAG ATGTACGAAG AGGAGATTCA GGAATGGTAC 
GAGGAATATT CCAGAACAAA CCTTCAGACT GATGGGACCT TCCACAGAGA ACTGGACAGC 
CCTGATGCTG GTGCCACAAA CAGCATTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165276.1
CDS	1684..4116
Translation	MDISSTFDNMDTGLTQDRPVQMSSFYKEIEPVDGAGNHDVVNNVTIPASGASDPRAQNSLQQSWEMNFQEAAIYLQEGENNDKFFTHPRSAHALSAYLFAHSHLFYVVELLTAVLLMLLSLSEAPAVPFLRLDVYVHATLELLALVMVAFELCMKLRWLGFHTFIRHKRTMVKTSVLFIQFIEAIVVLVRQTSHLRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFAILGFCLFSANTADHYFSTLENSIVSLFVLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQRPDGVSLKQFDGLMRFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKGINLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIALAFDMEPFYFIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFETEVSQTEALQTLDFYKHTLGVTNLTSLQDMCVALERSGTSLVFLGRRSRTKSDLSMKMYEEEIQEWYEEYSRTNLQTDGTFHRELDSPDAGATNSIN

Target ORF information:

RefSeq Version	XM_005165219.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165219.4

ORF Insert Sequence:

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGACATCAT TAGTGTTTCT GGGTCGAAGG 
TCACGTACCA AGAGTGACCT CAGTATGAAG ATGTACGAAG AGGAGATTCA GGAATGGTAC 
GAGGAATATT CCAGAACAAA CCTTCAGACT GATGGGACCT TCCACAGAGA ACTGGACAGC 
CCTGATGCTG GTGCCACAAA CAGCATTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_005165219.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165276.1 Danio rerio two pore segment channel 1 (tpcn1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10362
Clone ID Related Accession (Same CDS sequence)	XM_005165219.4 , XM_005165219.3
Accession Version	XM_005165219.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	two pore calcium channel protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165219.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGACATCAT TAGTGTTTCT GGGTCGAAGG 
TCACGTACCA AGAGTGACCT CAGTATGAAG ATGTACGAAG AGGAGATTCA GGAATGGTAC 
GAGGAATATT CCAGAACAAA CCTTCAGACT GATGGGACCT TCCACAGAGA ACTGGACAGC 
CCTGATGCTG GTGCCACAAA CAGCATTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165276.1
CDS	1362..3794
Translation	MDISSTFDNMDTGLTQDRPVQMSSFYKEIEPVDGAGNHDVVNNVTIPASGASDPRAQNSLQQSWEMNFQEAAIYLQEGENNDKFFTHPRSAHALSAYLFAHSHLFYVVELLTAVLLMLLSLSEAPAVPFLRLDVYVHATLELLALVMVAFELCMKLRWLGFHTFIRHKRTMVKTSVLFIQFIEAIVVLVRQTSHLRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFAILGFCLFSANTADHYFSTLENSIVSLFVLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQRPDGVSLKQFDGLMRFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKGINLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIALAFDMEPFYFIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFETEVSQTEALQTLDFYKHTLGVTNLTSLQDMCVALERSGTSLVFLGRRSRTKSDLSMKMYEEEIQEWYEEYSRTNLQTDGTFHRELDSPDAGATNSIN

Target ORF information:

RefSeq Version	XM_005165219.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165219.3

ORF Insert Sequence:

ATGGATATTA GTAGTACATT TGACAACATG GACACTGGAC TGACACAAGA CAGACCAGTA 
CAAATGTCCT CATTCTACAA AGAGATTGAG CCTGTTGATG GAGCAGGAAA TCATGATGTT 
GTAAATAATG TCACCATCCC CGCCTCAGGA GCATCAGATC CTAGGGCCCA GAATTCTTTG 
CAGCAGAGCT GGGAAATGAA CTTCCAAGAA GCAGCAATAT ACCTGCAGGA GGGAGAGAAT 
AACGACAAGT TTTTCACTCA TCCTCGAAGT GCCCATGCGC TCAGTGCCTA CCTGTTTGCT 
CACAGTCATC TGTTCTATGT GGTGGAGCTT CTGACTGCGG TGTTACTCAT GCTCCTGTCC 
CTCTCAGAGG CTCCTGCCGT CCCCTTCCTC CGCCTGGACG TCTATGTTCA CGCAACACTG 
GAGTTATTGG CTTTGGTAAT GGTGGCCTTT GAGTTGTGTA TGAAGCTCCG ATGGCTTGGC 
TTCCACACTT TTATACGACA TAAGAGGACC ATGGTGAAGA CGAGTGTCCT GTTTATTCAG 
TTTATTGAGG CGATTGTGGT CTTAGTCCGG CAGACGTCCC ACCTGCGCGT CACAAGAGCC 
CTGCGACCAA TCTTTTTGGT GGACTGCCGT TACTGTGGAG CCGTTCGCAG AAACTTGAGG 
CAGATATTTC AGTCTCTTCC TCCCTTCATT GATATTCTTC TGCTGTTGCT TTTCTTCATG 
GTCATCTTTG CCATCCTGGG ATTCTGTTTG TTCTCAGCAA ACACAGCCGA TCATTATTTT 
AGCACACTGG AGAACAGCAT TGTGAGTCTG TTCGTCCTCC TGACCACTGC GAATTTTCCA 
GATGTGATGA TGCCGGCGTA TTCGAAGAAT CGTTGGTCAT GCATTTTCTT CATCGTGTAT 
CTGTCCATCG AGCTCTACTT CATCATGAAT CTGCTCCTAG CGGTGGTTTT TGACACGTTT 
AATGGAGTTG AGAAGATGAA GTTCAAATCT CTACTTCTAC ACAAGCGATC AGCCATCGAG 
CATGCCTTCC AGCTGTTGGT TAGTCGGCAG AGGCCAGATG GCGTGTCTTT GAAACAGTTT 
GATGGACTGA TGCGATTTTA TCATCCACGA ATGACTCCAC GTGATCGCTT TCTTACTTTT 
AAAGCCCTCA ACCATTCAGG TGCACCCATG CTGAGTCTCC AGGACTTCTA TAAATTTTAT 
GAAGTGATCG GACTGAAGTG GAAGGCACGA CGAAGTGGAG AGCACTGGTT TGATGATCTT 
CCTCGCACTG CATTGCTCAT TTTTAAAGGA ATCAATTTAC TTGTGAAATC GAAAGCTTTC 
CAATATGCAA TGTATCTGGT AGTAGCCGTC AATGGCATCT GGATCCTAGT GGAGACAAAC 
ATGTTAAATG ATGGCATCTC ATGGACTCGT GTCGTCCCCT GGAGTTACAT TATTTTCCTT 
ACAATTTATG GTATTGAGGT GTTGTTGAAG ATCACAGGCC TCGGACCCAT CACATATTTC 
AGCTCAGGAT GGAACCTCTT TGATTTCTCA GTAACAGTCT TTGCTTTTCT GGGACTGATT 
GCTCTGGCCT TTGACATGGA GCCGTTCTAC TTCATTGTGG TGTTGAGACC TCTTCAGCTT 
CTCCGGTTAT TTAAAATTAA ACAGCGCTAT CGGAATGTTC TGGACACAAT GTTTGAACTG 
TTCCCGCGCA TGGCCAGTCT AGGTCTGACT CTGATCATCT TCTATTACTC TTTTGCCATC 
GTCGGAATGG AGTTTTTTGC TGATGTTATT TATCCGAACT GCTGCGACAA TAGCACCGTC 
GCGGCCTCCT ATCGGCAGAT AAATGTCACC ACTGGCAACC GGACCGAATT ACAAGAGGGA 
TATTACTATC TCAATAACTT CAATAACATC CTAAGCAGCT TTGTAACTCT TTTTGAGCTG 
ACAGTGGTGA ACAACTGGTA CATCACAATG GAAGGTGTGA CATCACAAAC TACTCACTGG 
AGCCGCCTTT ATTTCATGAC CTTCTACATC GTGACCATGG TCGTCATGAC TATAATCGTG 
GCCTTCATAC TGGATGCATT TGTCTTTAGG ATGAATTACA GCCGGAAGAA CAGAGAGCCG 
CTGGATGACC CTGAAGATGA AAAGGGGATT GTGTTTGAGA CTGAAGTGTC CCAGACTGAA 
GCTCTGCAGA CTCTGGACTT TTATAAACAC ACACTGGGCG TCACCAACCT CACCTCTCTG 
CAGGACATGT GTGTGGCTCT GGAGCGAAGC GGGACATCAT TAGTGTTTCT GGGTCGAAGG 
TCACGTACCA AGAGTGACCT CAGTATGAAG ATGTACGAAG AGGAGATTCA GGAATGGTAC 
GAGGAATATT CCAGAACAAA CCTTCAGACT GATGGGACCT TCCACAGAGA ACTGGACAGC 
CCTGATGCTG GTGCCACAAA CAGCATTAAC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_005165220.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165277.1 Danio rerio two pore segment channel 1 (tpcn1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10363
Clone ID Related Accession (Same CDS sequence)	XM_005165220.3 , XM_005165220.2
Accession Version	XM_005165220.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	two pore calcium channel protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165220.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG GGGACGACGA CGTGCCCCTT ATCCTGACCT GGGACGATGC GAGCAGCGGG 
CTGCTGGAGG AAGATGAGAA TGGAGCAGGA AATCATGATG TTGTAAATAA TGTCACCATC 
CCCGCCTCAG GAGCATCAGA TCCTAGGGCC CAGAATTCTT TGCAGCAGAG CTGGGAAATG 
AACTTCCAAG AAGCAGCAAT ATACCTGCAG GAGGGAGAGA ATAACGACAA GTTTTTCACT 
CATCCTCGAA GTGCCCATGC GCTCAGTGCC TACCTGTTTG CTCACAGTCA TCTGTTCTAT 
GTGGTGGAGC TTCTGACTGC GGTGTTACTC ATGCTCCTGT CCCTCTCAGA GGCTCCTGCC 
GTCCCCTTCC TCCGCCTGGA CGTCTATGTT CACGCAACAC TGGAGTTATT GGCTTTGGTA 
ATGGTGGCCT TTGAGTTGTG TATGAAGCTC CGATGGCTTG GCTTCCACAC TTTTATACGA 
CATAAGAGGA CCATGGTGAA GACGAGTGTC CTGTTTATTC AGTTTATTGA GGCGATTGTG 
GTCTTAGTCC GGCAGACGTC CCACCTGCGC GTCACAAGAG CCCTGCGACC AATCTTTTTG 
GTGGACTGCC GTTACTGTGG AGCCGTTCGC AGAAACTTGA GGCAGATATT TCAGTCTCTT 
CCTCCCTTCA TTGATATTCT TCTGCTGTTG CTTTTCTTCA TGGTCATCTT TGCCATCCTG 
GGATTCTGTT TGTTCTCAGC AAACACAGCC GATCATTATT TTAGCACACT GGAGAACAGC 
ATTGTGAGTC TGTTCGTCCT CCTGACCACT GCGAATTTTC CAGATGTGAT GATGCCGGCG 
TATTCGAAGA ATCGTTGGTC ATGCATTTTC TTCATCGTGT ATCTGTCCAT CGAGCTCTAC 
TTCATCATGA ATCTGCTCCT AGCGGTGGTT TTTGACACGT TTAATGGAGT TGAGAAGATG 
AAGTTCAAAT CTCTACTTCT ACACAAGCGA TCAGCCATCG AGCATGCCTT CCAGCTGTTG 
GTTAGTCGGC AGAGGCCAGA TGGCGTGTCT TTGAAACAGT TTGATGGACT GATGCGATTT 
TATCATCCAC GAATGACTCC ACGTGATCGC TTTCTTACTT TTAAAGCCCT CAACCATTCA 
GGTGCACCCA TGCTGAGTCT CCAGGACTTC TATAAATTTT ATGAAGTGAT CGGACTGAAG 
TGGAAGGCAC GACGAAGTGG AGAGCACTGG TTTGATGATC TTCCTCGCAC TGCATTGCTC 
ATTTTTAAAG GAATCAATTT ACTTGTGAAA TCGAAAGCTT TCCAATATGC AATGTATCTG 
GTAGTAGCCG TCAATGGCAT CTGGATCCTA GTGGAGACAA ACATGTTAAA TGATGGCATC 
TCATGGACTC GTGTCGTCCC CTGGAGTTAC ATTATTTTCC TTACAATTTA TGGTATTGAG 
GTGTTGTTGA AGATCACAGG CCTCGGACCC ATCACATATT TCAGCTCAGG ATGGAACCTC 
TTTGATTTCT CAGTAACAGT CTTTGCTTTT CTGGGACTGA TTGCTCTGGC CTTTGACATG 
GAGCCGTTCT ACTTCATTGT GGTGTTGAGA CCTCTTCAGC TTCTCCGGTT ATTTAAAATT 
AAACAGCGCT ATCGGAATGT TCTGGACACA ATGTTTGAAC TGTTCCCGCG CATGGCCAGT 
CTAGGTCTGA CTCTGATCAT CTTCTATTAC TCTTTTGCCA TCGTCGGAAT GGAGTTTTTT 
GCTGATGTTA TTTATCCGAA CTGCTGCGAC AATAGCACCG TCGCGGCCTC CTATCGGCAG 
ATAAATGTCA CCACTGGCAA CCGGACCGAA TTACAAGAGG GATATTACTA TCTCAATAAC 
TTCAATAACA TCCTAAGCAG CTTTGTAACT CTTTTTGAGC TGACAGTGGT GAACAACTGG 
TACATCACAA TGGAAGGTGT GACATCACAA ACTACTCACT GGAGCCGCCT TTATTTCATG 
ACCTTCTACA TCGTGACCAT GGTCGTCATG ACTATAATCG TGGCCTTCAT ACTGGATGCA 
TTTGTCTTTA GGATGAATTA CAGCCGGAAG AACAGAGAGC CGCTGGATGA CCCTGAAGAT 
GAAAAGGGGA TTGTGTTTGA GACTGAAGTG TCCCAGACTG AAGCTCTGCA GACTCTGGAC 
TTTTATAAAC ACACACTGGG CGTCACCAAC CTCACCTCTC TGCAGGACAT GTGTGTGGCT 
CTGGAGCGAA GCGGGCAGAC ATCATTAGTG TTTCTGGGTC GAAGGTCACG TACCAAGAGT 
GACCTCAGTA TGAAGATGTA CGAAGAGGAG ATTCAGGAAT GGTACGAGGA ATATTCCAGA 
ACAAACCTTC AGACTGATGG GACCTTCCAC AGAGAACTGG ACAGCCCTGA TGCTGGTGCC 
ACAAACAGCA TTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165277.1
CDS	424..2841
Translation	MADGDDDVPLILTWDDASSGLLEEDENGAGNHDVVNNVTIPASGASDPRAQNSLQQSWEMNFQEAAIYLQEGENNDKFFTHPRSAHALSAYLFAHSHLFYVVELLTAVLLMLLSLSEAPAVPFLRLDVYVHATLELLALVMVAFELCMKLRWLGFHTFIRHKRTMVKTSVLFIQFIEAIVVLVRQTSHLRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFAILGFCLFSANTADHYFSTLENSIVSLFVLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQRPDGVSLKQFDGLMRFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKGINLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIALAFDMEPFYFIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFETEVSQTEALQTLDFYKHTLGVTNLTSLQDMCVALERSGQTSLVFLGRRSRTKSDLSMKMYEEEIQEWYEEYSRTNLQTDGTFHRELDSPDAGATNSIN

Target ORF information:

RefSeq Version	XM_005165220.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165220.3

ORF Insert Sequence:

ATGGCGGACG GGGACGACGA CGTGCCCCTT ATCCTGACCT GGGACGATGC GAGCAGCGGG 
CTGCTGGAGG AAGATGAGAA TGGAGCAGGA AATCATGATG TTGTAAATAA TGTCACCATC 
CCCGCCTCAG GAGCATCAGA TCCTAGGGCC CAGAATTCTT TGCAGCAGAG CTGGGAAATG 
AACTTCCAAG AAGCAGCAAT ATACCTGCAG GAGGGAGAGA ATAACGACAA GTTTTTCACT 
CATCCTCGAA GTGCCCATGC GCTCAGTGCC TACCTGTTTG CTCACAGTCA TCTGTTCTAT 
GTGGTGGAGC TTCTGACTGC GGTGTTACTC ATGCTCCTGT CCCTCTCAGA GGCTCCTGCC 
GTCCCCTTCC TCCGCCTGGA CGTCTATGTT CACGCAACAC TGGAGTTATT GGCTTTGGTA 
ATGGTGGCCT TTGAGTTGTG TATGAAGCTC CGATGGCTTG GCTTCCACAC TTTTATACGA 
CATAAGAGGA CCATGGTGAA GACGAGTGTC CTGTTTATTC AGTTTATTGA GGCGATTGTG 
GTCTTAGTCC GGCAGACGTC CCACCTGCGC GTCACAAGAG CCCTGCGACC AATCTTTTTG 
GTGGACTGCC GTTACTGTGG AGCCGTTCGC AGAAACTTGA GGCAGATATT TCAGTCTCTT 
CCTCCCTTCA TTGATATTCT TCTGCTGTTG CTTTTCTTCA TGGTCATCTT TGCCATCCTG 
GGATTCTGTT TGTTCTCAGC AAACACAGCC GATCATTATT TTAGCACACT GGAGAACAGC 
ATTGTGAGTC TGTTCGTCCT CCTGACCACT GCGAATTTTC CAGATGTGAT GATGCCGGCG 
TATTCGAAGA ATCGTTGGTC ATGCATTTTC TTCATCGTGT ATCTGTCCAT CGAGCTCTAC 
TTCATCATGA ATCTGCTCCT AGCGGTGGTT TTTGACACGT TTAATGGAGT TGAGAAGATG 
AAGTTCAAAT CTCTACTTCT ACACAAGCGA TCAGCCATCG AGCATGCCTT CCAGCTGTTG 
GTTAGTCGGC AGAGGCCAGA TGGCGTGTCT TTGAAACAGT TTGATGGACT GATGCGATTT 
TATCATCCAC GAATGACTCC ACGTGATCGC TTTCTTACTT TTAAAGCCCT CAACCATTCA 
GGTGCACCCA TGCTGAGTCT CCAGGACTTC TATAAATTTT ATGAAGTGAT CGGACTGAAG 
TGGAAGGCAC GACGAAGTGG AGAGCACTGG TTTGATGATC TTCCTCGCAC TGCATTGCTC 
ATTTTTAAAG GAATCAATTT ACTTGTGAAA TCGAAAGCTT TCCAATATGC AATGTATCTG 
GTAGTAGCCG TCAATGGCAT CTGGATCCTA GTGGAGACAA ACATGTTAAA TGATGGCATC 
TCATGGACTC GTGTCGTCCC CTGGAGTTAC ATTATTTTCC TTACAATTTA TGGTATTGAG 
GTGTTGTTGA AGATCACAGG CCTCGGACCC ATCACATATT TCAGCTCAGG ATGGAACCTC 
TTTGATTTCT CAGTAACAGT CTTTGCTTTT CTGGGACTGA TTGCTCTGGC CTTTGACATG 
GAGCCGTTCT ACTTCATTGT GGTGTTGAGA CCTCTTCAGC TTCTCCGGTT ATTTAAAATT 
AAACAGCGCT ATCGGAATGT TCTGGACACA ATGTTTGAAC TGTTCCCGCG CATGGCCAGT 
CTAGGTCTGA CTCTGATCAT CTTCTATTAC TCTTTTGCCA TCGTCGGAAT GGAGTTTTTT 
GCTGATGTTA TTTATCCGAA CTGCTGCGAC AATAGCACCG TCGCGGCCTC CTATCGGCAG 
ATAAATGTCA CCACTGGCAA CCGGACCGAA TTACAAGAGG GATATTACTA TCTCAATAAC 
TTCAATAACA TCCTAAGCAG CTTTGTAACT CTTTTTGAGC TGACAGTGGT GAACAACTGG 
TACATCACAA TGGAAGGTGT GACATCACAA ACTACTCACT GGAGCCGCCT TTATTTCATG 
ACCTTCTACA TCGTGACCAT GGTCGTCATG ACTATAATCG TGGCCTTCAT ACTGGATGCA 
TTTGTCTTTA GGATGAATTA CAGCCGGAAG AACAGAGAGC CGCTGGATGA CCCTGAAGAT 
GAAAAGGGGA TTGTGTTTGA GACTGAAGTG TCCCAGACTG AAGCTCTGCA GACTCTGGAC 
TTTTATAAAC ACACACTGGG CGTCACCAAC CTCACCTCTC TGCAGGACAT GTGTGTGGCT 
CTGGAGCGAA GCGGGCAGAC ATCATTAGTG TTTCTGGGTC GAAGGTCACG TACCAAGAGT 
GACCTCAGTA TGAAGATGTA CGAAGAGGAG ATTCAGGAAT GGTACGAGGA ATATTCCAGA 
ACAAACCTTC AGACTGATGG GACCTTCCAC AGAGAACTGG ACAGCCCTGA TGCTGGTGCC 
ACAAACAGCA TTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_005165220.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165277.1 Danio rerio two pore segment channel 1 (tpcn1), transcript variant X3, mRNA.

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CloneID	ODa10363
Clone ID Related Accession (Same CDS sequence)	XM_005165220.3 , XM_005165220.2
Accession Version	XM_005165220.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	two pore calcium channel protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACG GGGACGACGA CGTGCCCCTT ATCCTGACCT GGGACGATGC GAGCAGCGGG 
CTGCTGGAGG AAGATGAGAA TGGAGCAGGA AATCATGATG TTGTAAATAA TGTCACCATC 
CCCGCCTCAG GAGCATCAGA TCCTAGGGCC CAGAATTCTT TGCAGCAGAG CTGGGAAATG 
AACTTCCAAG AAGCAGCAAT ATACCTGCAG GAGGGAGAGA ATAACGACAA GTTTTTCACT 
CATCCTCGAA GTGCCCATGC GCTCAGTGCC TACCTGTTTG CTCACAGTCA TCTGTTCTAT 
GTGGTGGAGC TTCTGACTGC GGTGTTACTC ATGCTCCTGT CCCTCTCAGA GGCTCCTGCC 
GTCCCCTTCC TCCGCCTGGA CGTCTATGTT CACGCAACAC TGGAGTTATT GGCTTTGGTA 
ATGGTGGCCT TTGAGTTGTG TATGAAGCTC CGATGGCTTG GCTTCCACAC TTTTATACGA 
CATAAGAGGA CCATGGTGAA GACGAGTGTC CTGTTTATTC AGTTTATTGA GGCGATTGTG 
GTCTTAGTCC GGCAGACGTC CCACCTGCGC GTCACAAGAG CCCTGCGACC AATCTTTTTG 
GTGGACTGCC GTTACTGTGG AGCCGTTCGC AGAAACTTGA GGCAGATATT TCAGTCTCTT 
CCTCCCTTCA TTGATATTCT TCTGCTGTTG CTTTTCTTCA TGGTCATCTT TGCCATCCTG 
GGATTCTGTT TGTTCTCAGC AAACACAGCC GATCATTATT TTAGCACACT GGAGAACAGC 
ATTGTGAGTC TGTTCGTCCT CCTGACCACT GCGAATTTTC CAGATGTGAT GATGCCGGCG 
TATTCGAAGA ATCGTTGGTC ATGCATTTTC TTCATCGTGT ATCTGTCCAT CGAGCTCTAC 
TTCATCATGA ATCTGCTCCT AGCGGTGGTT TTTGACACGT TTAATGGAGT TGAGAAGATG 
AAGTTCAAAT CTCTACTTCT ACACAAGCGA TCAGCCATCG AGCATGCCTT CCAGCTGTTG 
GTTAGTCGGC AGAGGCCAGA TGGCGTGTCT TTGAAACAGT TTGATGGACT GATGCGATTT 
TATCATCCAC GAATGACTCC ACGTGATCGC TTTCTTACTT TTAAAGCCCT CAACCATTCA 
GGTGCACCCA TGCTGAGTCT CCAGGACTTC TATAAATTTT ATGAAGTGAT CGGACTGAAG 
TGGAAGGCAC GACGAAGTGG AGAGCACTGG TTTGATGATC TTCCTCGCAC TGCATTGCTC 
ATTTTTAAAG GAATCAATTT ACTTGTGAAA TCGAAAGCTT TCCAATATGC AATGTATCTG 
GTAGTAGCCG TCAATGGCAT CTGGATCCTA GTGGAGACAA ACATGTTAAA TGATGGCATC 
TCATGGACTC GTGTCGTCCC CTGGAGTTAC ATTATTTTCC TTACAATTTA TGGTATTGAG 
GTGTTGTTGA AGATCACAGG CCTCGGACCC ATCACATATT TCAGCTCAGG ATGGAACCTC 
TTTGATTTCT CAGTAACAGT CTTTGCTTTT CTGGGACTGA TTGCTCTGGC CTTTGACATG 
GAGCCGTTCT ACTTCATTGT GGTGTTGAGA CCTCTTCAGC TTCTCCGGTT ATTTAAAATT 
AAACAGCGCT ATCGGAATGT TCTGGACACA ATGTTTGAAC TGTTCCCGCG CATGGCCAGT 
CTAGGTCTGA CTCTGATCAT CTTCTATTAC TCTTTTGCCA TCGTCGGAAT GGAGTTTTTT 
GCTGATGTTA TTTATCCGAA CTGCTGCGAC AATAGCACCG TCGCGGCCTC CTATCGGCAG 
ATAAATGTCA CCACTGGCAA CCGGACCGAA TTACAAGAGG GATATTACTA TCTCAATAAC 
TTCAATAACA TCCTAAGCAG CTTTGTAACT CTTTTTGAGC TGACAGTGGT GAACAACTGG 
TACATCACAA TGGAAGGTGT GACATCACAA ACTACTCACT GGAGCCGCCT TTATTTCATG 
ACCTTCTACA TCGTGACCAT GGTCGTCATG ACTATAATCG TGGCCTTCAT ACTGGATGCA 
TTTGTCTTTA GGATGAATTA CAGCCGGAAG AACAGAGAGC CGCTGGATGA CCCTGAAGAT 
GAAAAGGGGA TTGTGTTTGA GACTGAAGTG TCCCAGACTG AAGCTCTGCA GACTCTGGAC 
TTTTATAAAC ACACACTGGG CGTCACCAAC CTCACCTCTC TGCAGGACAT GTGTGTGGCT 
CTGGAGCGAA GCGGGCAGAC ATCATTAGTG TTTCTGGGTC GAAGGTCACG TACCAAGAGT 
GACCTCAGTA TGAAGATGTA CGAAGAGGAG ATTCAGGAAT GGTACGAGGA ATATTCCAGA 
ACAAACCTTC AGACTGATGG GACCTTCCAC AGAGAACTGG ACAGCCCTGA TGCTGGTGCC 
ACAAACAGCA TTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165277.1
CDS	428..2845
Translation	MADGDDDVPLILTWDDASSGLLEEDENGAGNHDVVNNVTIPASGASDPRAQNSLQQSWEMNFQEAAIYLQEGENNDKFFTHPRSAHALSAYLFAHSHLFYVVELLTAVLLMLLSLSEAPAVPFLRLDVYVHATLELLALVMVAFELCMKLRWLGFHTFIRHKRTMVKTSVLFIQFIEAIVVLVRQTSHLRVTRALRPIFLVDCRYCGAVRRNLRQIFQSLPPFIDILLLLLFFMVIFAILGFCLFSANTADHYFSTLENSIVSLFVLLTTANFPDVMMPAYSKNRWSCIFFIVYLSIELYFIMNLLLAVVFDTFNGVEKMKFKSLLLHKRSAIEHAFQLLVSRQRPDGVSLKQFDGLMRFYHPRMTPRDRFLTFKALNHSGAPMLSLQDFYKFYEVIGLKWKARRSGEHWFDDLPRTALLIFKGINLLVKSKAFQYAMYLVVAVNGIWILVETNMLNDGISWTRVVPWSYIIFLTIYGIEVLLKITGLGPITYFSSGWNLFDFSVTVFAFLGLIALAFDMEPFYFIVVLRPLQLLRLFKIKQRYRNVLDTMFELFPRMASLGLTLIIFYYSFAIVGMEFFADVIYPNCCDNSTVAASYRQINVTTGNRTELQEGYYYLNNFNNILSSFVTLFELTVVNNWYITMEGVTSQTTHWSRLYFMTFYIVTMVVMTIIVAFILDAFVFRMNYSRKNREPLDDPEDEKGIVFETEVSQTEALQTLDFYKHTLGVTNLTSLQDMCVALERSGQTSLVFLGRRSRTKSDLSMKMYEEEIQEWYEEYSRTNLQTDGTFHRELDSPDAGATNSIN

Target ORF information:

RefSeq Version	XM_005165220.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio two pore segment channel 1 (tpcn1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165220.2

ORF Insert Sequence:

ATGGCGGACG GGGACGACGA CGTGCCCCTT ATCCTGACCT GGGACGATGC GAGCAGCGGG 
CTGCTGGAGG AAGATGAGAA TGGAGCAGGA AATCATGATG TTGTAAATAA TGTCACCATC 
CCCGCCTCAG GAGCATCAGA TCCTAGGGCC CAGAATTCTT TGCAGCAGAG CTGGGAAATG 
AACTTCCAAG AAGCAGCAAT ATACCTGCAG GAGGGAGAGA ATAACGACAA GTTTTTCACT 
CATCCTCGAA GTGCCCATGC GCTCAGTGCC TACCTGTTTG CTCACAGTCA TCTGTTCTAT 
GTGGTGGAGC TTCTGACTGC GGTGTTACTC ATGCTCCTGT CCCTCTCAGA GGCTCCTGCC 
GTCCCCTTCC TCCGCCTGGA CGTCTATGTT CACGCAACAC TGGAGTTATT GGCTTTGGTA 
ATGGTGGCCT TTGAGTTGTG TATGAAGCTC CGATGGCTTG GCTTCCACAC TTTTATACGA 
CATAAGAGGA CCATGGTGAA GACGAGTGTC CTGTTTATTC AGTTTATTGA GGCGATTGTG 
GTCTTAGTCC GGCAGACGTC CCACCTGCGC GTCACAAGAG CCCTGCGACC AATCTTTTTG 
GTGGACTGCC GTTACTGTGG AGCCGTTCGC AGAAACTTGA GGCAGATATT TCAGTCTCTT 
CCTCCCTTCA TTGATATTCT TCTGCTGTTG CTTTTCTTCA TGGTCATCTT TGCCATCCTG 
GGATTCTGTT TGTTCTCAGC AAACACAGCC GATCATTATT TTAGCACACT GGAGAACAGC 
ATTGTGAGTC TGTTCGTCCT CCTGACCACT GCGAATTTTC CAGATGTGAT GATGCCGGCG 
TATTCGAAGA ATCGTTGGTC ATGCATTTTC TTCATCGTGT ATCTGTCCAT CGAGCTCTAC 
TTCATCATGA ATCTGCTCCT AGCGGTGGTT TTTGACACGT TTAATGGAGT TGAGAAGATG 
AAGTTCAAAT CTCTACTTCT ACACAAGCGA TCAGCCATCG AGCATGCCTT CCAGCTGTTG 
GTTAGTCGGC AGAGGCCAGA TGGCGTGTCT TTGAAACAGT TTGATGGACT GATGCGATTT 
TATCATCCAC GAATGACTCC ACGTGATCGC TTTCTTACTT TTAAAGCCCT CAACCATTCA 
GGTGCACCCA TGCTGAGTCT CCAGGACTTC TATAAATTTT ATGAAGTGAT CGGACTGAAG 
TGGAAGGCAC GACGAAGTGG AGAGCACTGG TTTGATGATC TTCCTCGCAC TGCATTGCTC 
ATTTTTAAAG GAATCAATTT ACTTGTGAAA TCGAAAGCTT TCCAATATGC AATGTATCTG 
GTAGTAGCCG TCAATGGCAT CTGGATCCTA GTGGAGACAA ACATGTTAAA TGATGGCATC 
TCATGGACTC GTGTCGTCCC CTGGAGTTAC ATTATTTTCC TTACAATTTA TGGTATTGAG 
GTGTTGTTGA AGATCACAGG CCTCGGACCC ATCACATATT TCAGCTCAGG ATGGAACCTC 
TTTGATTTCT CAGTAACAGT CTTTGCTTTT CTGGGACTGA TTGCTCTGGC CTTTGACATG 
GAGCCGTTCT ACTTCATTGT GGTGTTGAGA CCTCTTCAGC TTCTCCGGTT ATTTAAAATT 
AAACAGCGCT ATCGGAATGT TCTGGACACA ATGTTTGAAC TGTTCCCGCG CATGGCCAGT 
CTAGGTCTGA CTCTGATCAT CTTCTATTAC TCTTTTGCCA TCGTCGGAAT GGAGTTTTTT 
GCTGATGTTA TTTATCCGAA CTGCTGCGAC AATAGCACCG TCGCGGCCTC CTATCGGCAG 
ATAAATGTCA CCACTGGCAA CCGGACCGAA TTACAAGAGG GATATTACTA TCTCAATAAC 
TTCAATAACA TCCTAAGCAG CTTTGTAACT CTTTTTGAGC TGACAGTGGT GAACAACTGG 
TACATCACAA TGGAAGGTGT GACATCACAA ACTACTCACT GGAGCCGCCT TTATTTCATG 
ACCTTCTACA TCGTGACCAT GGTCGTCATG ACTATAATCG TGGCCTTCAT ACTGGATGCA 
TTTGTCTTTA GGATGAATTA CAGCCGGAAG AACAGAGAGC CGCTGGATGA CCCTGAAGAT 
GAAAAGGGGA TTGTGTTTGA GACTGAAGTG TCCCAGACTG AAGCTCTGCA GACTCTGGAC 
TTTTATAAAC ACACACTGGG CGTCACCAAC CTCACCTCTC TGCAGGACAT GTGTGTGGCT 
CTGGAGCGAA GCGGGCAGAC ATCATTAGTG TTTCTGGGTC GAAGGTCACG TACCAAGAGT 
GACCTCAGTA TGAAGATGTA CGAAGAGGAG ATTCAGGAAT GGTACGAGGA ATATTCCAGA 
ACAAACCTTC AGACTGATGG GACCTTCCAC AGAGAACTGG ACAGCCCTGA TGCTGGTGCC 
ACAAACAGCA TTAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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TPCN1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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