TPCN1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011538492.1	XP_011536794.1	two pore calcium channel protein 1 isoform X1
NM_001143819.2	NP_001137291.1	two pore calcium channel protein 1 isoform 1
XM_011538490.2	XP_011536792.1	two pore calcium channel protein 1 isoform X1
XM_017019480.2	XP_016874969.1	two pore calcium channel protein 1 isoform X2
NM_017901.6	NP_060371.2	two pore calcium channel protein 1 isoform 2
NM_001301214.1	NP_001288143.1	two pore calcium channel protein 1 isoform 3
XM_011538489.1	XP_011536791.1	two pore calcium channel protein 1 isoform X4
XM_017019480.1	XP_016874969.1	two pore calcium channel protein 1 isoform X2
NM_001351346.1	NP_001338275.1	two pore calcium channel protein 1 isoform 3
NM_017901.5	NP_060371.2	two pore calcium channel protein 1 isoform 2
XM_011538493.2	XP_011536795.1	two pore calcium channel protein 1 isoform X4
XM_017019482.2	XP_016874971.1	two pore calcium channel protein 1 isoform X5
XM_017019482.1	XP_016874971.1	two pore calcium channel protein 1 isoform X5
NM_001143819.3	NP_001137291.1	two pore calcium channel protein 1 isoform 1
XM_011538492.2	XP_011536794.1	two pore calcium channel protein 1 isoform X3
NM_001351347.1	NP_001338276.1	two pore calcium channel protein 1 isoform 3
NM_001351346.2	NP_001338275.1	two pore calcium channel protein 1 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu02615	NM_017901.6 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu02615	XM_011538492.2 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu68190	XM_011538489.1 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu46641	NM_001351347.1 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu46641	NM_001351346.2 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu46641	NM_001301214.1 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu46641	XM_011538493.2 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu31800	NM_001143819.3 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu31800	XM_011538490.2 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu83894	XM_017019480.2 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu83895	XM_017019482.2 Latest version!	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu83894	XM_017019480.1	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu02615	NM_017901.5	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu83895	XM_017019482.1	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu31800	NM_001143819.2	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu02615	XM_011538492.1	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu46641	NM_001351346.1	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
Hide Old Accession Versions >>

TPCN1 ( NM_017901.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060371.2 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02615
Clone ID Related Accession (Same CDS sequence)	XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
Accession Version	NM_017901.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1 and BC150203.1. On Jun 2, 2019 this sequence version replaced NM_017901.5. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150203.1, AB032995.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060371.2
CDS	289..2739
Translation	MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_017901.6
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017901.6

ORF Insert Sequence:

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_017901.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060371.2 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu02615
Clone ID Related Accession (Same CDS sequence)	XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
Accession Version	NM_017901.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1 and BC150203.1. On Jul 25, 2014 this sequence version replaced NM_017901.4. Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150203.1, AK093490.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060371.2
CDS	315..2765
Translation	MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_017901.5
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017901.5

ORF Insert Sequence:

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011538492.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536794.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.

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CloneID	OHu02615
Clone ID Related Accession (Same CDS sequence)	XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
Accession Version	XM_011538492.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	two pore calcium channel protein 1 isoform X1
Comment

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011536794.1

Translation

MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011538492.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538492.1

ORF Insert Sequence:

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011538492.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536794.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.

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CloneID	OHu02615
Clone ID Related Accession (Same CDS sequence)	XM_011538492.1 , NM_017901.6 , NM_017901.5 , XM_011538492.2
Accession Version	XM_011538492.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2451bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536794.1
CDS	221..2671
Translation	MAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011538492.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538492.2

ORF Insert Sequence:

ATGGCTGTGA GTTTGGATGA CGACGTGCCG CTCATCCTGA CCTTGGATGA GGGTGGCAGT 
GCCCCACTGG CTCCCTCCAA CGGCCTGGGC CAAGAAGAGC TACCTAGCAA AAATGGCGGC 
AGCTATGCCA TCCACGACTC CCAGGCCCCC AGTCTCAGCT CTGGGGGTGA GAGTTCCCCC 
TCCAGCCCCG CACACAACTG GGAGATGAAT TACCAAGAGG CAGCAATCTA CCTCCAGGAA 
GGCGAGAACA ACGACAAGTT CTTCACCCAC CCCAAGGATG CCAAGGCGCT GGCGGCCTAC 
CTCTTTGCAC ACAATCACCT CTTCTACCTG ATGGAGCTGG CCACGGCCCT GCTGCTGCTG 
CTGCTCTCCC TGTGCGAGGC CCCCGCCGTC CCCGCACTCC GGCTTGGCAT CTATGTCCAC 
GCCACCCTGG AGCTGTTTGC CCTGATGGTG GTAGTGTTTG AACTCTGCAT GAAGTTACGC 
TGGCTGGGCC TCCACACCTT CATCCGGCAC AAGCGGACCA TGGTCAAGAC CTCGGTGCTG 
GTGGTGCAGT TTGTCGAGGC CATCGTGGTG TTGGTACGGC AGATGTCCCA TGTGCGGGTG 
ACCCGAGCAC TGCGCTGCAT TTTCCTGGTG GACTGTCGGT ATTGCGGTGG CGTCCGGCGC 
AACCTGCGGC AGATCTTCCA GTCCCTGCCG CCCTTCATGG ACATCCTCCT GCTGCTGCTG 
TTCTTCATGA TCATCTTTGC CATCCTCGGT TTCTACTTGT TCTCCCCTAA CCCTTCAGAC 
CCCTACTTCA GCACCCTGGA GAACAGCATC GTCAGTCTGT TTGTCCTTCT GACCACAGCC 
AATTTCCCAG ATGTGATGAT GCCCTCCTAC TCCCGGAACC CCTGGTCCTG CGTCTTCTTC 
ATCGTGTACC TCTCCATCGA GCTGTATTTC ATCATGAACC TGCTTCTGGC TGTGGTGTTC 
GACACCTTCA ATGACATTGA GAAACGCAAG TTCAAGTCTT TGCTACTGCA CAAGCGAACC 
GCTATCCAGC ATGCCTACCG CCTGCTCATC AGCCAGAGGA GGCCTGCCGG CATCTCCTAC 
AGGCAGTTTG AAGGCCTCAT GCGCTTCTAC AAGCCCCGGA TGAGTGCCAG GGAGCGCTAT 
CTTACCTTCA AGGCCCTGAA TCAGAACAAC ACACCCCTGC TCAGCCTAAA GGACTTTTAC 
GATATCTACG AAGTTGCTGC TTTGAAGTGG AAGGCCAAGA AAAACAGAGA GCACTGGTTT 
GATGAGCTTC CCAGGACGGC GCTCCTCATC TTCAAAGGTA TTAATATCCT TGTGAAGTCC 
AAGGCCTTCC AGTATTTCAT GTACTTGGTG GTGGCAGTCA ACGGGGTCTG GATCCTCGTG 
GAGACATTTA TGCTGAAAGG TGGGAACTTC TTCTCCAAGC ACGTGCCCTG GAGTTACCTC 
GTCTTTCTAA CTATCTATGG GGTGGAGCTG TTCCTGAAGG TTGCCGGCCT GGGCCCTGTG 
GAGTACTTGT CTTCCGGATG GAACTTGTTT GACTTCTCCG TGACAGTGTT CGCCTTCCTG 
GGACTGCTGG CGCTGGCCCT CAACATGGAG CCCTTCTATT TCATCGTGGT CCTGCGCCCC 
CTCCAGCTGC TGAGGTTGTT TAAGTTGAAG GAGCGCTACC GCAACGTGCT GGACACCATG 
TTCGAGCTGC TGCCCCGGAT GGCCAGCCTG GGCCTCACCC TGCTCATCTT TTACTACTCC 
TTCGCCATCG TGGGCATGGA GTTCTTCTGC GGGATCGTCT TCCCCAACTG CTGCAACACG 
AGTACAGTGG CAGATGCCTA CCGCTGGCGC AACCACACCG TGGGCAACAG GACCGTGGTG 
GAGGAAGGCT ACTATTATCT CAATAATTTT GACAACATCC TCAACAGCTT TGTGACCCTG 
TTTGAGCTCA CAGTTGTCAA CAACTGGTAC ATCATCATGG AAGGCGTCAC CTCTCAGACC 
TCCCACTGGA GCCGCCTCTA CTTCATGACC TTTTACATTG TGACCATGGT GGTGATGACG 
ATCATTGTCG CCTTTATCCT CGAGGCCTTC GTCTTCCGAA TGAACTACAG CCGCAAGAAC 
CAGGACTCGG AAGTTGATGG TGGCATCACC CTTGAGAAGG AAATCTCCAA AGAAGAGCTG 
GTTGCCGTCC TGGAGCTCTA CCGGGAGGCA CGGGGGGCCT CCTCGGATGT CACCAGGCTG 
CTGGAGACCC TCTCCCAGAT GGAGAGATAC CAGCAACATT CCATGGTGTT TCTGGGACGG 
CGATCAAGGA CCAAGAGCGA CCTGAGCCTG AAGATGTACC AGGAGGAGAT CCAGGAGTGG 
TATGAGGAGC ATGCCAGGGA GCAAGAGCAG CAGCGACAAC TCAGCAGCAG TGCAGCCCCC 
GCCGCCCAGC AGCCCCCAGG CAGCCGCCAG CGCTCCCAGA CCGTTACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011538489.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536791.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu68190
Clone ID Related Accession (Same CDS sequence)	XM_011538489.1
Accession Version	XM_011538489.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2709bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	two pore calcium channel protein 1 isoform X4
Comment

ATGGAAAGAC TTGGGACTCC CTCTGGGTTG GACTCAACGA AGATGGAGTC TTGCTACATT 
GCCCAGGCTG GTCTTGAACT GCTAGGCTCA AGCAGTTCTC CCACCTTGAC CTCCCAAAGC 
GCTGAGATTA CAGAAGATGC CTCTAATGGA GGAGTTTCTG AGCAGCACCC CTGGCCCAGT 
GGCTTTGAAA GGGAGCTCAA ACCAGAGACT ATTTCAAGCC CTGGATATCA TATCCTGAGG 
GCCACAGGAG AAGAGAACAT GGCTGTGAGT TTGGATGACG ACGTGCCGCT CATCCTGACC 
TTGGATGAGG GTGGCAGTGC CCCACTGGCT CCCTCCAACG GCCTGGGCCA AGAAGAGCTA 
CCTAGCAAAA ATGGCGGCAG CTATGCCATC CACGACTCCC AGGCCCCCAG TCTCAGCTCT 
GGGGGTGAGA GTTCCCCCTC CAGCCCCGCA CACAACTGGG AGATGAATTA CCAAGAGGCA 
GCAATCTACC TCCAGGAAGG CGAGAACAAC GACAAGTTCT TCACCCACCC CAAGGATGCC 
AAGGCGCTGG CGGCCTACCT CTTTGCACAC AATCACCTCT TCTACCTGAT GGAGCTGGCC 
ACGGCCCTGC TGCTGCTGCT GCTCTCCCTG TGCGAGGCCC CCGCCGTCCC CGCACTCCGG 
CTTGGCATCT ATGTCCACGC CACCCTGGAG CTGTTTGCCC TGATGGTGGT AGTGTTTGAA 
CTCTGCATGA AGTTACGCTG GCTGGGCCTC CACACCTTCA TCCGGCACAA GCGGACCATG 
GTCAAGACCT CGGTGCTGGT GGTGCAGTTT GTCGAGGCCA TCGTGGTGTT GGTACGGCAG 
ATGTCCCATG TGCGGGTGAC CCGAGCACTG CGCTGCATTT TCCTGGTGGA CTGTCGGTAT 
TGCGGTGGCG TCCGGCGCAA CCTGCGGCAG ATCTTCCAGT CCCTGCCGCC CTTCATGGAC 
ATCCTCCTGC TGCTGCTGTT CTTCATGATC ATCTTTGCCA TCCTCGGTTT CTACTTGTTC 
TCCCCTAACC CTTCAGACCC CTACTTCAGC ACCCTGGAGA ACAGCATCGT CAGTCTGTTT 
GTCCTTCTGA CCACAGCCAA TTTCCCAGAT GTGATGATGC CCTCCTACTC CCGGAACCCC 
TGGTCCTGCG TCTTCTTCAT CGTGTACCTC TCCATCGAGC TGTATTTCAT CATGAACCTG 
CTTCTGGCTG TGGTGTTCGA CACCTTCAAT GACATTGAGA AACGCAAGTT CAAGTCTTTG 
CTACTGCACA AGCGAACCGC TATCCAGCAT GCCTACCGCC TGCTCATCAG CCAGAGGAGG 
CCTGCCGGCA TCTCCTACAG GCAGTTTGAA GGCCTCATGC GCTTCTACAA GCCCCGGATG 
AGTGCCAGGG AGCGCTATCT TACCTTCAAG GCCCTGAATC AGAACAACAC ACCCCTGCTC 
AGCCTAAAGG ACTTTTACGA TATCTACGAA GTTGCTGCTT TGAAGTGGAA GGCCAAGAAA 
AACAGAGAGC ACTGGTTTGA TGAGCTTCCC AGGACGGCGC TCCTCATCTT CAAAGGTATT 
AATATCCTTG TGAAGTCCAA GGCCTTCCAG TATTTCATGT ACTTGGTGGT GGCAGTCAAC 
GGGGTCTGGA TCCTCGTGGA GACATTTATG CTGAAAGGTG GGAACTTCTT CTCCAAGCAC 
GTGCCCTGGA GTTACCTCGT CTTTCTAACT ATCTATGGGG TGGAGCTGTT CCTGAAGGTT 
GCCGGCCTGG GCCCTGTGGA GTACTTGTCT TCCGGATGGA ACTTGTTTGA CTTCTCCGTG 
ACAGTGTTCG CCTTCCTGGG ACTGCTGGCG CTGGCCCTCA ACATGGAGCC CTTCTATTTC 
ATCGTGGTCC TGCGCCCCCT CCAGCTGCTG AGGTTGTTTA AGTTGAAGGA GCGCTACCGC 
AACGTGCTGG ACACCATGTT CGAGCTGCTG CCCCGGATGG CCAGCCTGGG CCTCACCCTG 
CTCATCTTTT ACTACTCCTT CGCCATCGTG GGCATGGAGT TCTTCTGCGG GATCGTCTTC 
CCCAACTGCT GCAACACGAG TACAGTGGCA GATGCCTACC GCTGGCGCAA CCACACCGTG 
GGCAACAGGA CCGTGGTGGA GGAAGGCTAC TATTATCTCA ATAATTTTGA CAACATCCTC 
AACAGCTTTG TGACCCTGTT TGAGCTCACA GTTGTCAACA ACTGGTACAT CATCATGGAA 
GGCGTCACCT CTCAGACCTC CCACTGGAGC CGCCTCTACT TCATGACCTT TTACATTGTG 
ACCATGGTGG TGATGACGAT CATTGTCGCC TTTATCCTCG AGGCCTTCGT CTTCCGAATG 
AACTACAGCC GCAAGAACCA GGACTCGGAA GTTGATGGTG GCATCACCCT TGAGAAGGAA 
ATCTCCAAAG AAGAGCTGGT TGCCGTCCTG GAGCTCTACC GGGAGGCACG GGGGGCCTCC 
TCGGATGTCA CCAGGCTGCT GGAGACCCTC TCCCAGATGG AGAGATACCA GCAACATTCC 
ATGGTGTTTC TGGGACGGCG ATCAAGGACC AAGAGCGACC TGAGCCTGAA GATGTACCAG 
GAGGAGATCC AGGAGTGGTA TGAGGAGCAT GCCAGGGAGC AAGAGCAGCA GCGACAACTC 
AGCAGCAGTG CAGCCCCCGC CGCCCAGCAG CCCCCAGGCA GCCGCCAGCG CTCCCAGACC 
GTTACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011536791.1

Translation

MERLGTPSGLDSTKMESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011538489.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538489.1

ORF Insert Sequence:

ATGGAAAGAC TTGGGACTCC CTCTGGGTTG GACTCAACGA AGATGGAGTC TTGCTACATT 
GCCCAGGCTG GTCTTGAACT GCTAGGCTCA AGCAGTTCTC CCACCTTGAC CTCCCAAAGC 
GCTGAGATTA CAGAAGATGC CTCTAATGGA GGAGTTTCTG AGCAGCACCC CTGGCCCAGT 
GGCTTTGAAA GGGAGCTCAA ACCAGAGACT ATTTCAAGCC CTGGATATCA TATCCTGAGG 
GCCACAGGAG AAGAGAACAT GGCTGTGAGT TTGGATGACG ACGTGCCGCT CATCCTGACC 
TTGGATGAGG GTGGCAGTGC CCCACTGGCT CCCTCCAACG GCCTGGGCCA AGAAGAGCTA 
CCTAGCAAAA ATGGCGGCAG CTATGCCATC CACGACTCCC AGGCCCCCAG TCTCAGCTCT 
GGGGGTGAGA GTTCCCCCTC CAGCCCCGCA CACAACTGGG AGATGAATTA CCAAGAGGCA 
GCAATCTACC TCCAGGAAGG CGAGAACAAC GACAAGTTCT TCACCCACCC CAAGGATGCC 
AAGGCGCTGG CGGCCTACCT CTTTGCACAC AATCACCTCT TCTACCTGAT GGAGCTGGCC 
ACGGCCCTGC TGCTGCTGCT GCTCTCCCTG TGCGAGGCCC CCGCCGTCCC CGCACTCCGG 
CTTGGCATCT ATGTCCACGC CACCCTGGAG CTGTTTGCCC TGATGGTGGT AGTGTTTGAA 
CTCTGCATGA AGTTACGCTG GCTGGGCCTC CACACCTTCA TCCGGCACAA GCGGACCATG 
GTCAAGACCT CGGTGCTGGT GGTGCAGTTT GTCGAGGCCA TCGTGGTGTT GGTACGGCAG 
ATGTCCCATG TGCGGGTGAC CCGAGCACTG CGCTGCATTT TCCTGGTGGA CTGTCGGTAT 
TGCGGTGGCG TCCGGCGCAA CCTGCGGCAG ATCTTCCAGT CCCTGCCGCC CTTCATGGAC 
ATCCTCCTGC TGCTGCTGTT CTTCATGATC ATCTTTGCCA TCCTCGGTTT CTACTTGTTC 
TCCCCTAACC CTTCAGACCC CTACTTCAGC ACCCTGGAGA ACAGCATCGT CAGTCTGTTT 
GTCCTTCTGA CCACAGCCAA TTTCCCAGAT GTGATGATGC CCTCCTACTC CCGGAACCCC 
TGGTCCTGCG TCTTCTTCAT CGTGTACCTC TCCATCGAGC TGTATTTCAT CATGAACCTG 
CTTCTGGCTG TGGTGTTCGA CACCTTCAAT GACATTGAGA AACGCAAGTT CAAGTCTTTG 
CTACTGCACA AGCGAACCGC TATCCAGCAT GCCTACCGCC TGCTCATCAG CCAGAGGAGG 
CCTGCCGGCA TCTCCTACAG GCAGTTTGAA GGCCTCATGC GCTTCTACAA GCCCCGGATG 
AGTGCCAGGG AGCGCTATCT TACCTTCAAG GCCCTGAATC AGAACAACAC ACCCCTGCTC 
AGCCTAAAGG ACTTTTACGA TATCTACGAA GTTGCTGCTT TGAAGTGGAA GGCCAAGAAA 
AACAGAGAGC ACTGGTTTGA TGAGCTTCCC AGGACGGCGC TCCTCATCTT CAAAGGTATT 
AATATCCTTG TGAAGTCCAA GGCCTTCCAG TATTTCATGT ACTTGGTGGT GGCAGTCAAC 
GGGGTCTGGA TCCTCGTGGA GACATTTATG CTGAAAGGTG GGAACTTCTT CTCCAAGCAC 
GTGCCCTGGA GTTACCTCGT CTTTCTAACT ATCTATGGGG TGGAGCTGTT CCTGAAGGTT 
GCCGGCCTGG GCCCTGTGGA GTACTTGTCT TCCGGATGGA ACTTGTTTGA CTTCTCCGTG 
ACAGTGTTCG CCTTCCTGGG ACTGCTGGCG CTGGCCCTCA ACATGGAGCC CTTCTATTTC 
ATCGTGGTCC TGCGCCCCCT CCAGCTGCTG AGGTTGTTTA AGTTGAAGGA GCGCTACCGC 
AACGTGCTGG ACACCATGTT CGAGCTGCTG CCCCGGATGG CCAGCCTGGG CCTCACCCTG 
CTCATCTTTT ACTACTCCTT CGCCATCGTG GGCATGGAGT TCTTCTGCGG GATCGTCTTC 
CCCAACTGCT GCAACACGAG TACAGTGGCA GATGCCTACC GCTGGCGCAA CCACACCGTG 
GGCAACAGGA CCGTGGTGGA GGAAGGCTAC TATTATCTCA ATAATTTTGA CAACATCCTC 
AACAGCTTTG TGACCCTGTT TGAGCTCACA GTTGTCAACA ACTGGTACAT CATCATGGAA 
GGCGTCACCT CTCAGACCTC CCACTGGAGC CGCCTCTACT TCATGACCTT TTACATTGTG 
ACCATGGTGG TGATGACGAT CATTGTCGCC TTTATCCTCG AGGCCTTCGT CTTCCGAATG 
AACTACAGCC GCAAGAACCA GGACTCGGAA GTTGATGGTG GCATCACCCT TGAGAAGGAA 
ATCTCCAAAG AAGAGCTGGT TGCCGTCCTG GAGCTCTACC GGGAGGCACG GGGGGCCTCC 
TCGGATGTCA CCAGGCTGCT GGAGACCCTC TCCCAGATGG AGAGATACCA GCAACATTCC 
ATGGTGTTTC TGGGACGGCG ATCAAGGACC AAGAGCGACC TGAGCCTGAA GATGTACCAG 
GAGGAGATCC AGGAGTGGTA TGAGGAGCAT GCCAGGGAGC AAGAGCAGCA GCGACAACTC 
AGCAGCAGTG CAGCCCCCGC CGCCCAGCAG CCCCCAGGCA GCCGCCAGCG CTCCCAGACC 
GTTACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_001301214.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288143.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu46641
Clone ID Related Accession (Same CDS sequence)	NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
Accession Version	NM_001301214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK296268.1 and BC150203.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses a downstream start codon, compared to variant 1. The encoded isoform (3) has a shorter N-terminus compared to isoform 1. Variants 3, 4, and 5 all encode the same isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK296268.1, SRR1660807.164670.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154361, SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288143.1
CDS	194..2440
Translation	MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_001301214.1
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301214.1

ORF Insert Sequence:

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_001351346.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338275.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu46641
Clone ID Related Accession (Same CDS sequence)	NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
Accession Version	NM_001351346.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-21
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC089999.20 and AC010178.40. On May 6, 2017 this sequence version replaced XM_017019481.1. Transcript Variant: This variant (4), as well as variants 3 and 5, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.52948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338275.1
CDS	780..3026
Translation	MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_001351346.1
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351346.1

ORF Insert Sequence:

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_001351347.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338276.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 5, mRNA.

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CloneID	OHu46641
Clone ID Related Accession (Same CDS sequence)	NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
Accession Version	NM_001351347.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-16
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC089999.20 and AC010178.40. On May 6, 2017 this sequence version replaced XM_005253906.4. Transcript Variant: This variant (5), as well as variants 3 and 4, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.215530.1, SRR1660809.229235.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338276.1
CDS	469..2715
Translation	MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_001351347.1
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351347.1

ORF Insert Sequence:

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_001351346.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001338275.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.

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CloneID	OHu46641
Clone ID Related Accession (Same CDS sequence)	NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
Accession Version	NM_001351346.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC089999.20 and AC010178.40. On Jun 2, 2019 this sequence version replaced NM_001351346.1. Transcript Variant: This variant (4), as well as variants 3 and 5, encodes isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.52948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001338275.1
CDS	754..3000
Translation	MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_001351346.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001351346.2

ORF Insert Sequence:

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011538493.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536795.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.

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CloneID	OHu46641
Clone ID Related Accession (Same CDS sequence)	NM_001301214.1 , NM_001351346.1 , XM_011538493.2 , NM_001351347.1 , NM_001351346.2
Accession Version	XM_011538493.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538493.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536795.1
CDS	269..2515
Translation	MNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011538493.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538493.2

ORF Insert Sequence:

ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_001143819.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137291.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.

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CloneID	OHu31800
Clone ID Related Accession (Same CDS sequence)	NM_001143819.2 , XM_011538490.2 , NM_001143819.3
Accession Version	NM_001143819.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1, AK057414.1 and BC150203.1. On Jul 25, 2014 this sequence version replaced NM_001143819.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK057414.1, SRR1660807.24185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137291.1
CDS	192..2858
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_001143819.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143819.2

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( NM_001143819.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001137291.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.

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CloneID	OHu31800
Clone ID Related Accession (Same CDS sequence)	NM_001143819.2 , XM_011538490.2 , NM_001143819.3
Accession Version	NM_001143819.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093490.1, AK057414.1 and BC150203.1. On Jun 2, 2019 this sequence version replaced NM_001143819.2. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.24185.1, AK057414.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137291.1
CDS	166..2832
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	NM_001143819.3
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143819.3

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_011538490.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536792.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

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CloneID	OHu31800
Clone ID Related Accession (Same CDS sequence)	NM_001143819.2 , XM_011538490.2 , NM_001143819.3
Accession Version	XM_011538490.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2667bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011538490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536792.1
CDS	158..2824
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_011538490.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011538490.2

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGCCTGGGCC TCACCCTGCT CATCTTTTAC TACTCCTTCG CCATCGTGGG CATGGAGTTC 
TTCTGCGGGA TCGTCTTCCC CAACTGCTGC AACACGAGTA CAGTGGCAGA TGCCTACCGC 
TGGCGCAACC ACACCGTGGG CAACAGGACC GTGGTGGAGG AAGGCTACTA TTATCTCAAT 
AATTTTGACA ACATCCTCAA CAGCTTTGTG ACCCTGTTTG AGCTCACAGT TGTCAACAAC 
TGGTACATCA TCATGGAAGG CGTCACCTCT CAGACCTCCC ACTGGAGCCG CCTCTACTTC 
ATGACCTTTT ACATTGTGAC CATGGTGGTG ATGACGATCA TTGTCGCCTT TATCCTCGAG 
GCCTTCGTCT TCCGAATGAA CTACAGCCGC AAGAACCAGG ACTCGGAAGT TGATGGTGGC 
ATCACCCTTG AGAAGGAAAT CTCCAAAGAA GAGCTGGTTG CCGTCCTGGA GCTCTACCGG 
GAGGCACGGG GGGCCTCCTC GGATGTCACC AGGCTGCTGG AGACCCTCTC CCAGATGGAG 
AGATACCAGC AACATTCCAT GGTGTTTCTG GGACGGCGAT CAAGGACCAA GAGCGACCTG 
AGCCTGAAGA TGTACCAGGA GGAGATCCAG GAGTGGTATG AGGAGCATGC CAGGGAGCAA 
GAGCAGCAGC GACAACTCAG CAGCAGTGCA GCCCCCGCCG CCCAGCAGCC CCCAGGCAGC 
CGCCAGCGCT CCCAGACCGT TACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_017019480.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874969.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83894
Clone ID Related Accession (Same CDS sequence)	XM_017019480.2 , XM_017019480.1
Accession Version	XM_017019480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT 
GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA 
TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC 
CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG 
TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG 
ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG 
GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC 
TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT 
CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC 
AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC 
TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC 
CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT 
GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC 
TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG 
ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC 
GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG 
GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA 
GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874969.1
CDS	170..2782
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKVYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_017019480.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019480.2

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT 
GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA 
TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC 
CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG 
TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG 
ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG 
GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC 
TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT 
CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC 
AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC 
TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC 
CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT 
GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC 
TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG 
ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC 
GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG 
GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA 
GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_017019480.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874969.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83894
Clone ID Related Accession (Same CDS sequence)	XM_017019480.2 , XM_017019480.1
Accession Version	XM_017019480.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT 
GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA 
TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC 
CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG 
TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG 
ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG 
GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC 
TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT 
CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC 
AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC 
TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC 
CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT 
GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC 
TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG 
ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC 
GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG 
GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA 
GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874969.1
CDS	118..2730
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKVYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMASLGLTLLIFYYSFAIVGMEFFCGIVFPNCCNTSTVADAYRWRNHTVGNRTVVEEGYYYLNNFDNILNSFVTLFELTVVNNWYIIMEGVTSQTSHWSRLYFMTFYIVTMVVMTIIVAFILEAFVFRMNYSRKNQDSEVDGGITLEKEISKEELVAVLELYREARGASSDVTRLLETLSQMERYQQHSMVFLGRRSRTKSDLSLKMYQEEIQEWYEEHAREQEQQRQLSSSAAPAAQQPPGSRQRSQTVT

Target ORF information:

RefSeq Version	XM_017019480.1
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019480.1

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGTCTAT 
GGGGTGGAGC TGTTCCTGAA GGTTGCCGGC CTGGGCCCTG TGGAGTACTT GTCTTCCGGA 
TGGAACTTGT TTGACTTCTC CGTGACAGTG TTCGCCTTCC TGGGACTGCT GGCGCTGGCC 
CTCAACATGG AGCCCTTCTA TTTCATCGTG GTCCTGCGCC CCCTCCAGCT GCTGAGGTTG 
TTTAAGTTGA AGGAGCGCTA CCGCAACGTG CTGGACACCA TGTTCGAGCT GCTGCCCCGG 
ATGGCCAGCC TGGGCCTCAC CCTGCTCATC TTTTACTACT CCTTCGCCAT CGTGGGCATG 
GAGTTCTTCT GCGGGATCGT CTTCCCCAAC TGCTGCAACA CGAGTACAGT GGCAGATGCC 
TACCGCTGGC GCAACCACAC CGTGGGCAAC AGGACCGTGG TGGAGGAAGG CTACTATTAT 
CTCAATAATT TTGACAACAT CCTCAACAGC TTTGTGACCC TGTTTGAGCT CACAGTTGTC 
AACAACTGGT ACATCATCAT GGAAGGCGTC ACCTCTCAGA CCTCCCACTG GAGCCGCCTC 
TACTTCATGA CCTTTTACAT TGTGACCATG GTGGTGATGA CGATCATTGT CGCCTTTATC 
CTCGAGGCCT TCGTCTTCCG AATGAACTAC AGCCGCAAGA ACCAGGACTC GGAAGTTGAT 
GGTGGCATCA CCCTTGAGAA GGAAATCTCC AAAGAAGAGC TGGTTGCCGT CCTGGAGCTC 
TACCGGGAGG CACGGGGGGC CTCCTCGGAT GTCACCAGGC TGCTGGAGAC CCTCTCCCAG 
ATGGAGAGAT ACCAGCAACA TTCCATGGTG TTTCTGGGAC GGCGATCAAG GACCAAGAGC 
GACCTGAGCC TGAAGATGTA CCAGGAGGAG ATCCAGGAGT GGTATGAGGA GCATGCCAGG 
GAGCAAGAGC AGCAGCGACA ACTCAGCAGC AGTGCAGCCC CCGCCGCCCA GCAGCCCCCA 
GGCAGCCGCC AGCGCTCCCA GACCGTTACC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_017019482.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874971.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu83895
Clone ID Related Accession (Same CDS sequence)	XM_017019482.2 , XM_017019482.1
Accession Version	XM_017019482.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017019482.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874971.1
CDS	170..2095
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMAR

Target ORF information:

RefSeq Version	XM_017019482.2
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019482.2

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TPCN1 ( XM_017019482.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874971.1 Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu83895
Clone ID Related Accession (Same CDS sequence)	XM_017019482.2 , XM_017019482.1
Accession Version	XM_017019482.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1926bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	two pore calcium channel protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016874971.1
CDS	119..2044
Translation	MESCYIAQAGLELLGSSSSPTLTSQSAEITEDASNGGVSEQHPWPSGFERELKPETISSPGYHILRATGEENMAVSLDDDVPLILTLDEGGSAPLAPSNGLGQEELPSKNGGSYAIHDSQAPSLSSGGESSPSSPAHNWEMNYQEAAIYLQEGENNDKFFTHPKDAKALAAYLFAHNHLFYLMELATALLLLLLSLCEAPAVPALRLGIYVHATLELFALMVVVFELCMKLRWLGLHTFIRHKRTMVKTSVLVVQFVEAIVVLVRQMSHVRVTRALRCIFLVDCRYCGGVRRNLRQIFQSLPPFMDILLLLLFFMIIFAILGFYLFSPNPSDPYFSTLENSIVSLFVLLTTANFPDVMMPSYSRNPWSCVFFIVYLSIELYFIMNLLLAVVFDTFNDIEKRKFKSLLLHKRTAIQHAYRLLISQRRPAGISYRQFEGLMRFYKPRMSARERYLTFKALNQNNTPLLSLKDFYDIYEVAALKWKAKKNREHWFDELPRTALLIFKGINILVKSKAFQYFMYLVVAVNGVWILVETFMLKGGNFFSKHVPWSYLVFLTIYGVELFLKVAGLGPVEYLSSGWNLFDFSVTVFAFLGLLALALNMEPFYFIVVLRPLQLLRLFKLKERYRNVLDTMFELLPRMAR

Target ORF information:

RefSeq Version	XM_017019482.1
Organism	Homo sapiens(human)
Definition	Homo sapiens two pore segment channel 1 (TPCN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017019482.1

ORF Insert Sequence:

ATGGAGTCTT GCTACATTGC CCAGGCTGGT CTTGAACTGC TAGGCTCAAG CAGTTCTCCC 
ACCTTGACCT CCCAAAGCGC TGAGATTACA GAAGATGCCT CTAATGGAGG AGTTTCTGAG 
CAGCACCCCT GGCCCAGTGG CTTTGAAAGG GAGCTCAAAC CAGAGACTAT TTCAAGCCCT 
GGATATCATA TCCTGAGGGC CACAGGAGAA GAGAACATGG CTGTGAGTTT GGATGACGAC 
GTGCCGCTCA TCCTGACCTT GGATGAGGGT GGCAGTGCCC CACTGGCTCC CTCCAACGGC 
CTGGGCCAAG AAGAGCTACC TAGCAAAAAT GGCGGCAGCT ATGCCATCCA CGACTCCCAG 
GCCCCCAGTC TCAGCTCTGG GGGTGAGAGT TCCCCCTCCA GCCCCGCACA CAACTGGGAG 
ATGAATTACC AAGAGGCAGC AATCTACCTC CAGGAAGGCG AGAACAACGA CAAGTTCTTC 
ACCCACCCCA AGGATGCCAA GGCGCTGGCG GCCTACCTCT TTGCACACAA TCACCTCTTC 
TACCTGATGG AGCTGGCCAC GGCCCTGCTG CTGCTGCTGC TCTCCCTGTG CGAGGCCCCC 
GCCGTCCCCG CACTCCGGCT TGGCATCTAT GTCCACGCCA CCCTGGAGCT GTTTGCCCTG 
ATGGTGGTAG TGTTTGAACT CTGCATGAAG TTACGCTGGC TGGGCCTCCA CACCTTCATC 
CGGCACAAGC GGACCATGGT CAAGACCTCG GTGCTGGTGG TGCAGTTTGT CGAGGCCATC 
GTGGTGTTGG TACGGCAGAT GTCCCATGTG CGGGTGACCC GAGCACTGCG CTGCATTTTC 
CTGGTGGACT GTCGGTATTG CGGTGGCGTC CGGCGCAACC TGCGGCAGAT CTTCCAGTCC 
CTGCCGCCCT TCATGGACAT CCTCCTGCTG CTGCTGTTCT TCATGATCAT CTTTGCCATC 
CTCGGTTTCT ACTTGTTCTC CCCTAACCCT TCAGACCCCT ACTTCAGCAC CCTGGAGAAC 
AGCATCGTCA GTCTGTTTGT CCTTCTGACC ACAGCCAATT TCCCAGATGT GATGATGCCC 
TCCTACTCCC GGAACCCCTG GTCCTGCGTC TTCTTCATCG TGTACCTCTC CATCGAGCTG 
TATTTCATCA TGAACCTGCT TCTGGCTGTG GTGTTCGACA CCTTCAATGA CATTGAGAAA 
CGCAAGTTCA AGTCTTTGCT ACTGCACAAG CGAACCGCTA TCCAGCATGC CTACCGCCTG 
CTCATCAGCC AGAGGAGGCC TGCCGGCATC TCCTACAGGC AGTTTGAAGG CCTCATGCGC 
TTCTACAAGC CCCGGATGAG TGCCAGGGAG CGCTATCTTA CCTTCAAGGC CCTGAATCAG 
AACAACACAC CCCTGCTCAG CCTAAAGGAC TTTTACGATA TCTACGAAGT TGCTGCTTTG 
AAGTGGAAGG CCAAGAAAAA CAGAGAGCAC TGGTTTGATG AGCTTCCCAG GACGGCGCTC 
CTCATCTTCA AAGGTATTAA TATCCTTGTG AAGTCCAAGG CCTTCCAGTA TTTCATGTAC 
TTGGTGGTGG CAGTCAACGG GGTCTGGATC CTCGTGGAGA CATTTATGCT GAAAGGTGGG 
AACTTCTTCT CCAAGCACGT GCCCTGGAGT TACCTCGTCT TTCTAACTAT CTATGGGGTG 
GAGCTGTTCC TGAAGGTTGC CGGCCTGGGC CCTGTGGAGT ACTTGTCTTC CGGATGGAAC 
TTGTTTGACT TCTCCGTGAC AGTGTTCGCC TTCCTGGGAC TGCTGGCGCT GGCCCTCAAC 
ATGGAGCCCT TCTATTTCAT CGTGGTCCTG CGCCCCCTCC AGCTGCTGAG GTTGTTTAAG 
TTGAAGGAGC GCTACCGCAA CGTGCTGGAC ACCATGTTCG AGCTGCTGCC CCGGATGGCC 
AGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TPCN1
Entrez Gene ID	53373
Full Name	two pore segment channel 1
Synonyms	TPC1
General protein information	Preferred Names two pore segment channel 1 Names two pore calcium channel protein 1 two-pore channel 1, homolog voltage-dependent calcium channel protein TPC1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.13
Summary	Voltage-gated Ca(2+) and Na+ channels have 4 homologous domains, each containing 6 transmembrane segments, S1 to S6. TPCN1 is similar to these channels, but it has only 2 domains containing S1 to S6 (Ishibashi et al., 2000 [PubMed 10753632]).[supplied by OMIM, Mar 2008].
Disorder MIM:	609666

Pan troglodytes (chimpanzee)	TPCN1	XP_003313998.1
Canis lupus familiaris (dog)	TPCN1	XP_534690.2
Bos taurus (cattle)	TPCN1	XP_002694563.1
Gallus gallus (chicken)	TPCN1	XP_004934547.1
Homo sapiens (human)	TPCN1	NP_001137291.1
Rattus norvegicus (Norway rat)	Tpcn1	NP_647548.2
Danio rerio (zebrafish)	tpcn1	XP_005165277.1
Xenopus tropicalis (tropical clawed frog)	tpcn1	XP_002934333.2
	TPCN1	XP_002798827.1
Mus musculus (house mouse)	Tpcn1	NP_665852.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TPCN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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