NFX1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFX1
Entrez Gene ID	4799
Full Name	nuclear transcription factor, X-box binding 1
Synonyms	NFX2,TEG-42,Tex42
General protein information	Preferred Names nuclear transcription factor, X-box binding 1 Names transcriptional repressor NF-X1 nuclear transcription factor, X box-binding protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p13.3
Summary	MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified. [provided by RefSeq, Jul 2008].
Disorder MIM:	603255

mRNA and Protein(s)

mRNA	Protein	Name
NM_002504.4	NP_002495.2	transcriptional repressor NF-X1 isoform 1
NM_001318758.1	NP_001305687.1	transcriptional repressor NF-X1 isoform 4
NM_147134.3	NP_667345.1	transcriptional repressor NF-X1 isoform 3
NM_002504.6	NP_002495.2	transcriptional repressor NF-X1 isoform 1
NM_147134.4	NP_667345.1	transcriptional repressor NF-X1 isoform 3
NM_002504.5	NP_002495.2	transcriptional repressor NF-X1 isoform 1
NM_001318758.2	NP_001305687.1	transcriptional repressor NF-X1 isoform 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFX1	XP_001159552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008728	XP_314840.4
Oryza sativa (rice)	Os06g0252300	NP_001057298.1
Bos taurus (cattle)	NFX1	NP_001095499.1
Rattus norvegicus (Norway rat)	Nfx1	NP_001019955.2
Drosophila melanogaster (fruit fly)	stc	NP_001260460.1
Kluyveromyces lactis	KLLA0E08317g	XP_454325.1
Xenopus tropicalis (tropical clawed frog)	nfx1	XP_002935327.2
Homo sapiens (human)	NFX1	NP_002495.2
Eremothecium gossypii	AGOS_ADL213W	NP_983883.2
Magnaporthe oryzae (rice blast fungus)	MGG_00137	XP_003718994.1
Arabidopsis thaliana (thale cress)	NFXL1	NP_172488.1
Gallus gallus (chicken)	NFX1	XP_004935125.1
Saccharomyces cerevisiae (baker's yeast)	FAP1	NP_014375.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.03	NP_588382.1
Canis lupus familiaris (dog)	NFX1	XP_005626763.1
Mus musculus (house mouse)	Nfx1	NP_076228.2
Danio rerio (zebrafish)	LOC562071	XP_690559.4
Caenorhabditis elegans (roundworm)	nfx-1	NP_498394.1

Gene Ontology
Bibliography

Related articles in PubMed

Human papillomavirus type 16 E6 and NFX1-123 mislocalize immune signaling proteins and downregulate immune gene expression in keratinocytes.
Levan J, Vliet-Gregg PA, Robinson KL, Katzenellenbogen RA
PloS one12(11)e0187514(2017)

Involvement of the NFX1-repressor complex in PKC-?-induced repression of hTERT transcription.
Yamashita S, Fujii K, Zhao C, Takagi H, Katakura Y
Journal of biochemistry160(5)309-313(2016 Nov)

Human papillomavirus 16E6 and NFX1-123 potentiate Notch signaling and differentiation without activating cellular arrest.
Vliet-Gregg PA, Hamilton JR, Katzenellenbogen RA
Virology47850-60(2015 Apr)

NFX1-123 and human papillomavirus 16E6 increase Notch expression in keratinocytes.
Vliet-Gregg PA, Hamilton JR, Katzenellenbogen RA
Journal of virology87(24)13741-50(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study identifies NFX1-123 as a critical host protein partner through which 16E6 is able to subvert the immune response and in turn permit a long-lived high-risk Human papillomavirus infection.
Title: Human papillomavirus type 16 E6 and NFX1-123 mislocalize immune signaling proteins and downregulate immune gene expression in keratinocytes.

The results showed that a repressor complex composed of NFX1-91, mSin3A and histone deacetylase 1 was involved in the PKC-delta-induced repression of the hTERT promoter, which resulted in the repression of hTERT transcription.
Title: Involvement of the NFX1-repressor complex in PKC-?-induced repression of hTERT transcription.

Human papillomavirus 16 E6 (16E6) interacts with the cellular protein NFX1-123, and they collaboratively increase the growth and differentiation master regulator, Notch1.
Title: Human papillomavirus 16E6 and NFX1-123 potentiate Notch signaling and differentiation without activating cellular arrest.

NFX1-123 and human papillomavirus 16E6 upregulated Notch 1 expression in keratinocytes.
Title: NFX1-123 and human papillomavirus 16E6 increase Notch expression in keratinocytes.

a mechanism for HPV16 E6 activation of the NFkappaB pathway through NFX1-91
Title: NFX1 plays a role in human papillomavirus type 16 E6 activation of NFkappaB activity.

The following NFX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19315	NM_002504.6 Latest version!	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10810	NM_147134.4 Latest version!	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$587.30 $839.00
OHu42738	NM_001318758.2 Latest version!	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu19315	NM_002504.4	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10810	NM_147134.3	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$587.30 $839.00
OHu19315	NM_002504.5	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu42738	NM_001318758.1	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFX1 ( NM_002504.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002495.2 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19315
Clone ID Related Accession (Same CDS sequence)	NM_002504.4 , NM_002504.6 , NM_002504.5
Accession Version	NM_002504.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	transcriptional repressor NF-X1 isoform 1
Comment

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGTTTCGG AGCAACATCC CCTGTCACCT GGTTGATATC 
TCTTGCGGAT TACCCTGCAG TGCCACGCTA CCATGTGGGA TGCACAAATG TCAGAGACTC 
TGTCACAAAG GGGAGTGTCT TGTGGATGAG CCCTGCAAGC AGCCCTGCAC CACCCCCAGA 
GCTGACTGTG GTCACCCGTG TATGGCACCC TGCCATACCA GCTCACCCTG CCCTGTGACT 
GCTTGTAAAG CTAAGGTAGA GCTACAGTGT GAATGTGGAC GAAGAAAAGA GATGGTGATT 
TGCTCTGAAG CATCTAGTAC TTATCAAAGA ATAGCTGCAA TCTCCATGGC CTCTAAGATA 
ACAGACATGC AGCTTGGAGG TTCAGTGGAG ATCAGCAAGT TAATTACCAA AAAGGAAGTT 
CATCAAGCCA GGCTGGAGTG TGATGAGGAG TGTTCAGCCT TGGAAAGGAA AAAGAGATTA 
GCAGAGGCAT TTCATATCAG TGAGGATTCT GATCCTTTCA ATATACGTTC TTCAGGGTCA 
AAATTCAGTG ATAGTTTGAA AGAAGATGCC AGGAAGGACT TAAAGTTTGT CAGTGACGTT 
GAGAAGGAAA TGGAAACCCT CGTGGAGGCC GTGAATAAGG GAAAGAATAG TAAGAAAAGC 
CACAGCTTCC CTCCCATGAA CAGAGACCAC CGCCGGATCA TCCATGACTT GGCCCAAGTT 
TATGGCCTGG AGAGCGTGAG CTATGACAGT GAACCGAAGC GCAATGTGGT GGTCACTGCC 
ATCAGGGGGA AGTCCGTTTG TCCTCCTACC ACGCTGACAG GTGTGCTTGA AAGGGAAATG 
CAGGCACGGC CTCCACCACC GATTCCTCAT CACAGACATC AGTCAGACAA GAATCCTGGG 
AGCAGTAATT TACAGAAAAT AACCAAGGAG CCAATAATTG ACTATTTTGA CGTCCAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_002495.2

Translation

MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

Target ORF information:

RefSeq Version	NM_002504.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002504.4

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGTTTCGG AGCAACATCC CCTGTCACCT GGTTGATATC 
TCTTGCGGAT TACCCTGCAG TGCCACGCTA CCATGTGGGA TGCACAAATG TCAGAGACTC 
TGTCACAAAG GGGAGTGTCT TGTGGATGAG CCCTGCAAGC AGCCCTGCAC CACCCCCAGA 
GCTGACTGTG GTCACCCGTG TATGGCACCC TGCCATACCA GCTCACCCTG CCCTGTGACT 
GCTTGTAAAG CTAAGGTAGA GCTACAGTGT GAATGTGGAC GAAGAAAAGA GATGGTGATT 
TGCTCTGAAG CATCTAGTAC TTATCAAAGA ATAGCTGCAA TCTCCATGGC CTCTAAGATA 
ACAGACATGC AGCTTGGAGG TTCAGTGGAG ATCAGCAAGT TAATTACCAA AAAGGAAGTT 
CATCAAGCCA GGCTGGAGTG TGATGAGGAG TGTTCAGCCT TGGAAAGGAA AAAGAGATTA 
GCAGAGGCAT TTCATATCAG TGAGGATTCT GATCCTTTCA ATATACGTTC TTCAGGGTCA 
AAATTCAGTG ATAGTTTGAA AGAAGATGCC AGGAAGGACT TAAAGTTTGT CAGTGACGTT 
GAGAAGGAAA TGGAAACCCT CGTGGAGGCC GTGAATAAGG GAAAGAATAG TAAGAAAAGC 
CACAGCTTCC CTCCCATGAA CAGAGACCAC CGCCGGATCA TCCATGACTT GGCCCAAGTT 
TATGGCCTGG AGAGCGTGAG CTATGACAGT GAACCGAAGC GCAATGTGGT GGTCACTGCC 
ATCAGGGGGA AGTCCGTTTG TCCTCCTACC ACGCTGACAG GTGTGCTTGA AAGGGAAATG 
CAGGCACGGC CTCCACCACC GATTCCTCAT CACAGACATC AGTCAGACAA GAATCCTGGG 
AGCAGTAATT TACAGAAAAT AACCAAGGAG CCAATAATTG ACTATTTTGA CGTCCAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_002504.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002495.2 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19315
Clone ID Related Accession (Same CDS sequence)	NM_002504.4 , NM_002504.6 , NM_002504.5
Accession Version	NM_002504.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	transcriptional repressor NF-X1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB076795.1, BP871782.1 and BC012151.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002504.5. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.172918.1, SRR1803613.51729.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000379540.8/ ENSP00000368856.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGTTTCGG AGCAACATCC CCTGTCACCT GGTTGATATC 
TCTTGCGGAT TACCCTGCAG TGCCACGCTA CCATGTGGGA TGCACAAATG TCAGAGACTC 
TGTCACAAAG GGGAGTGTCT TGTGGATGAG CCCTGCAAGC AGCCCTGCAC CACCCCCAGA 
GCTGACTGTG GTCACCCGTG TATGGCACCC TGCCATACCA GCTCACCCTG CCCTGTGACT 
GCTTGTAAAG CTAAGGTAGA GCTACAGTGT GAATGTGGAC GAAGAAAAGA GATGGTGATT 
TGCTCTGAAG CATCTAGTAC TTATCAAAGA ATAGCTGCAA TCTCCATGGC CTCTAAGATA 
ACAGACATGC AGCTTGGAGG TTCAGTGGAG ATCAGCAAGT TAATTACCAA AAAGGAAGTT 
CATCAAGCCA GGCTGGAGTG TGATGAGGAG TGTTCAGCCT TGGAAAGGAA AAAGAGATTA 
GCAGAGGCAT TTCATATCAG TGAGGATTCT GATCCTTTCA ATATACGTTC TTCAGGGTCA 
AAATTCAGTG ATAGTTTGAA AGAAGATGCC AGGAAGGACT TAAAGTTTGT CAGTGACGTT 
GAGAAGGAAA TGGAAACCCT CGTGGAGGCC GTGAATAAGG GAAAGAATAG TAAGAAAAGC 
CACAGCTTCC CTCCCATGAA CAGAGACCAC CGCCGGATCA TCCATGACTT GGCCCAAGTT 
TATGGCCTGG AGAGCGTGAG CTATGACAGT GAACCGAAGC GCAATGTGGT GGTCACTGCC 
ATCAGGGGGA AGTCCGTTTG TCCTCCTACC ACGCTGACAG GTGTGCTTGA AAGGGAAATG 
CAGGCACGGC CTCCACCACC GATTCCTCAT CACAGACATC AGTCAGACAA GAATCCTGGG 
AGCAGTAATT TACAGAAAAT AACCAAGGAG CCAATAATTG ACTATTTTGA CGTCCAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002495.2
CDS	58..3420
Translation	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

Target ORF information:

RefSeq Version	NM_002504.6
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002504.6

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGTTTCGG AGCAACATCC CCTGTCACCT GGTTGATATC 
TCTTGCGGAT TACCCTGCAG TGCCACGCTA CCATGTGGGA TGCACAAATG TCAGAGACTC 
TGTCACAAAG GGGAGTGTCT TGTGGATGAG CCCTGCAAGC AGCCCTGCAC CACCCCCAGA 
GCTGACTGTG GTCACCCGTG TATGGCACCC TGCCATACCA GCTCACCCTG CCCTGTGACT 
GCTTGTAAAG CTAAGGTAGA GCTACAGTGT GAATGTGGAC GAAGAAAAGA GATGGTGATT 
TGCTCTGAAG CATCTAGTAC TTATCAAAGA ATAGCTGCAA TCTCCATGGC CTCTAAGATA 
ACAGACATGC AGCTTGGAGG TTCAGTGGAG ATCAGCAAGT TAATTACCAA AAAGGAAGTT 
CATCAAGCCA GGCTGGAGTG TGATGAGGAG TGTTCAGCCT TGGAAAGGAA AAAGAGATTA 
GCAGAGGCAT TTCATATCAG TGAGGATTCT GATCCTTTCA ATATACGTTC TTCAGGGTCA 
AAATTCAGTG ATAGTTTGAA AGAAGATGCC AGGAAGGACT TAAAGTTTGT CAGTGACGTT 
GAGAAGGAAA TGGAAACCCT CGTGGAGGCC GTGAATAAGG GAAAGAATAG TAAGAAAAGC 
CACAGCTTCC CTCCCATGAA CAGAGACCAC CGCCGGATCA TCCATGACTT GGCCCAAGTT 
TATGGCCTGG AGAGCGTGAG CTATGACAGT GAACCGAAGC GCAATGTGGT GGTCACTGCC 
ATCAGGGGGA AGTCCGTTTG TCCTCCTACC ACGCTGACAG GTGTGCTTGA AAGGGAAATG 
CAGGCACGGC CTCCACCACC GATTCCTCAT CACAGACATC AGTCAGACAA GAATCCTGGG 
AGCAGTAATT TACAGAAAAT AACCAAGGAG CCAATAATTG ACTATTTTGA CGTCCAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_002504.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002495.2 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.

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CloneID	OHu19315
Clone ID Related Accession (Same CDS sequence)	NM_002504.4 , NM_002504.6 , NM_002504.5
Accession Version	NM_002504.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	transcriptional repressor NF-X1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB076795.1, BP871782.1, BC012151.1 and AI884552.1. This sequence is a reference standard in the RefSeqGene project. On Jan 14, 2016 this sequence version replaced NM_002504.4. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.51729.1, SRR1660805.171157.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGTTTCGG AGCAACATCC CCTGTCACCT GGTTGATATC 
TCTTGCGGAT TACCCTGCAG TGCCACGCTA CCATGTGGGA TGCACAAATG TCAGAGACTC 
TGTCACAAAG GGGAGTGTCT TGTGGATGAG CCCTGCAAGC AGCCCTGCAC CACCCCCAGA 
GCTGACTGTG GTCACCCGTG TATGGCACCC TGCCATACCA GCTCACCCTG CCCTGTGACT 
GCTTGTAAAG CTAAGGTAGA GCTACAGTGT GAATGTGGAC GAAGAAAAGA GATGGTGATT 
TGCTCTGAAG CATCTAGTAC TTATCAAAGA ATAGCTGCAA TCTCCATGGC CTCTAAGATA 
ACAGACATGC AGCTTGGAGG TTCAGTGGAG ATCAGCAAGT TAATTACCAA AAAGGAAGTT 
CATCAAGCCA GGCTGGAGTG TGATGAGGAG TGTTCAGCCT TGGAAAGGAA AAAGAGATTA 
GCAGAGGCAT TTCATATCAG TGAGGATTCT GATCCTTTCA ATATACGTTC TTCAGGGTCA 
AAATTCAGTG ATAGTTTGAA AGAAGATGCC AGGAAGGACT TAAAGTTTGT CAGTGACGTT 
GAGAAGGAAA TGGAAACCCT CGTGGAGGCC GTGAATAAGG GAAAGAATAG TAAGAAAAGC 
CACAGCTTCC CTCCCATGAA CAGAGACCAC CGCCGGATCA TCCATGACTT GGCCCAAGTT 
TATGGCCTGG AGAGCGTGAG CTATGACAGT GAACCGAAGC GCAATGTGGT GGTCACTGCC 
ATCAGGGGGA AGTCCGTTTG TCCTCCTACC ACGCTGACAG GTGTGCTTGA AAGGGAAATG 
CAGGCACGGC CTCCACCACC GATTCCTCAT CACAGACATC AGTCAGACAA GAATCCTGGG 
AGCAGTAATT TACAGAAAAT AACCAAGGAG CCAATAATTG ACTATTTTGA CGTCCAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002495.2
CDS	157..3519
Translation	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

Target ORF information:

RefSeq Version	NM_002504.5
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002504.5

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGTTTCGG AGCAACATCC CCTGTCACCT GGTTGATATC 
TCTTGCGGAT TACCCTGCAG TGCCACGCTA CCATGTGGGA TGCACAAATG TCAGAGACTC 
TGTCACAAAG GGGAGTGTCT TGTGGATGAG CCCTGCAAGC AGCCCTGCAC CACCCCCAGA 
GCTGACTGTG GTCACCCGTG TATGGCACCC TGCCATACCA GCTCACCCTG CCCTGTGACT 
GCTTGTAAAG CTAAGGTAGA GCTACAGTGT GAATGTGGAC GAAGAAAAGA GATGGTGATT 
TGCTCTGAAG CATCTAGTAC TTATCAAAGA ATAGCTGCAA TCTCCATGGC CTCTAAGATA 
ACAGACATGC AGCTTGGAGG TTCAGTGGAG ATCAGCAAGT TAATTACCAA AAAGGAAGTT 
CATCAAGCCA GGCTGGAGTG TGATGAGGAG TGTTCAGCCT TGGAAAGGAA AAAGAGATTA 
GCAGAGGCAT TTCATATCAG TGAGGATTCT GATCCTTTCA ATATACGTTC TTCAGGGTCA 
AAATTCAGTG ATAGTTTGAA AGAAGATGCC AGGAAGGACT TAAAGTTTGT CAGTGACGTT 
GAGAAGGAAA TGGAAACCCT CGTGGAGGCC GTGAATAAGG GAAAGAATAG TAAGAAAAGC 
CACAGCTTCC CTCCCATGAA CAGAGACCAC CGCCGGATCA TCCATGACTT GGCCCAAGTT 
TATGGCCTGG AGAGCGTGAG CTATGACAGT GAACCGAAGC GCAATGTGGT GGTCACTGCC 
ATCAGGGGGA AGTCCGTTTG TCCTCCTACC ACGCTGACAG GTGTGCTTGA AAGGGAAATG 
CAGGCACGGC CTCCACCACC GATTCCTCAT CACAGACATC AGTCAGACAA GAATCCTGGG 
AGCAGTAATT TACAGAAAAT AACCAAGGAG CCAATAATTG ACTATTTTGA CGTCCAGGAC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_147134.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_667345.1 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10810
Clone ID Related Accession (Same CDS sequence)	NM_147134.3 , NM_147134.4
Accession Version	NM_147134.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	transcriptional repressor NF-X1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB076795.1, DA290058.1, AK225750.1 and AL356472.17. On Jan 14, 2016 this sequence version replaced NM_147134.2. Transcript Variant: This variant (3) utilizes an alternate 3' terminal exon compared to variant 1 and encodes the shortest isoform (3), with a unique C-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225750.1, SRR1803614.3150.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGCAGTCC CACTACTGGG CGTCTACCCA GAAGAAAAGA 
AGTCATTATA TGAAAAAGAT ACCTGCACAC GCATGTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_667345.1
CDS	157..2658
Translation	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEQSHYWASTQKKRSHYMKKIPAHACL

Target ORF information:

RefSeq Version	NM_147134.3
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147134.3

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGCAGTCC CACTACTGGG CGTCTACCCA GAAGAAAAGA 
AGTCATTATA TGAAAAAGAT ACCTGCACAC GCATGTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_147134.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_667345.1 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10810
Clone ID Related Accession (Same CDS sequence)	NM_147134.3 , NM_147134.4
Accession Version	NM_147134.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	transcriptional repressor NF-X1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB076795.1, DA290058.1, AK225750.1 and AL356472.17. On May 31, 2019 this sequence version replaced NM_147134.3. Transcript Variant: This variant (3) utilizes an alternate 3' terminal exon compared to variant 1 and encodes the shortest isoform (3), with a unique C-terminus. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1784470.1, SRR1803612.75925.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGCAGTCC CACTACTGGG CGTCTACCCA GAAGAAAAGA 
AGTCATTATA TGAAAAAGAT ACCTGCACAC GCATGTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_667345.1
CDS	58..2559
Translation	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEQSHYWASTQKKRSHYMKKIPAHACL

Target ORF information:

RefSeq Version	NM_147134.4
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_147134.4

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGATGCT 
ACATTTATGT GTGACAAGCG GTGTAACAAG AAACGGTTGT GTGGACGGCA TAAATGTAAT 
GAGATATGCT GTGTGGATAA GGAGCACAAG TGTCCTTTGA TTTGTGGGAG GAAACTCCGT 
TGTGGCCTTC ATAGGTGTGA AGAACCTTGT CATCGTGGAA ACTGCCAGAC ATGCTGGCAA 
GCCAGTTTTG ATGAATTAAC CTGCCATTGT GGTGCATCAG TGATTTACCC TCCAGTTCCC 
TGTGGTACTA GGCCCCCTGA ATGTACCCAA ACCTGCGCTA GAGTCCATGA GTGTGACCAT 
CCAGTATATC ATTCTTGTCA TAGTGAGGAG AAGTGTCCCC CTTGCACTTT CCTAACTCAG 
AAGTGGTGCA TGGGCAAGCA TGAGCAGTCC CACTACTGGG CGTCTACCCA GAAGAAAAGA 
AGTCATTATA TGAAAAAGAT ACCTGCACAC GCATGTTTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_001318758.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305687.1 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 4, mRNA.

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CloneID	OHu42738
Clone ID Related Accession (Same CDS sequence)	NM_001318758.1 , NM_001318758.2
Accession Version	NM_001318758.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	transcriptional repressor NF-X1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB076795.1, DA290058.1, AL832342.1, AL356472.17, AL356218.22 and AI884552.1. On Jan 14, 2016 this sequence version replaced XM_005251473.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in an internal exon, compared to variant 1. It encodes isoform 4 which is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.178823.1, AL832342.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGCAGAT 
GCTACATTTA TGTGTGACAA GCGGTGTAAC AAGAAACGGT TGTGTGGACG GCATAAATGT 
AATGAGATAT GCTGTGTGGA TAAGGAGCAC AAGTGTCCTT TGATTTGTGG GAGGAAACTC 
CGTTGTGGCC TTCATAGGTG TGAAGAACCT TGTCATCGTG GAAACTGCCA GACATGCTGG 
CAAGCCAGTT TTGATGAATT AACCTGCCAT TGTGGTGCAT CAGTGATTTA CCCTCCAGTT 
CCCTGTGGTA CTAGGCCCCC TGAATGTACC CAAACCTGCG CTAGAGTCCA TGAGTGTGAC 
CATCCAGTAT ATCATTCTTG TCATAGTGAG GAGAAGTGTC CCCCTTGCAC TTTCCTAACT 
CAGAAGTGGT GCATGGGCAA GCATGAGTTT CGGAGCAACA TCCCCTGTCA CCTGGTTGAT 
ATCTCTTGCG GATTACCCTG CAGTGCCACG CTACCATGTG GGATGCACAA ATGTCAGAGA 
CTCTGTCACA AAGGGGAGTG TCTTGTGGAT GAGCCCTGCA AGCAGCCCTG CACCACCCCC 
AGAGCTGACT GTGGTCACCC GTGTATGGCA CCCTGCCATA CCAGCTCACC CTGCCCTGTG 
ACTGCTTGTA AAGCTAAGGT AGAGCTACAG TGTGAATGTG GACGAAGAAA AGAGATGGTG 
ATTTGCTCTG AAGCATCTAG TACTTATCAA AGAATAGCTG CAATCTCCAT GGCCTCTAAG 
ATAACAGACA TGCAGCTTGG AGGTTCAGTG GAGATCAGCA AGTTAATTAC CAAAAAGGAA 
GTTCATCAAG CCAGGCTGGA GTGTGATGAG GAGTGTTCAG CCTTGGAAAG GAAAAAGAGA 
TTAGCAGAGG CATTTCATAT CAGTGAGGAT TCTGATCCTT TCAATATACG TTCTTCAGGG 
TCAAAATTCA GTGATAGTTT GAAAGAAGAT GCCAGGAAGG ACTTAAAGTT TGTCAGTGAC 
GTTGAGAAGG AAATGGAAAC CCTCGTGGAG GCCGTGAATA AGGGAAAGAA TAGTAAGAAA 
AGCCACAGCT TCCCTCCCAT GAACAGAGAC CACCGCCGGA TCATCCATGA CTTGGCCCAA 
GTTTATGGCC TGGAGAGCGT GAGCTATGAC AGTGAACCGA AGCGCAATGT GGTGGTCACT 
GCCATCAGGG GGAAGTCCGT TTGTCCTCCT ACCACGCTGA CAGGTGTGCT TGAAAGGGAA 
ATGCAGGCAC GGCCTCCACC ACCGATTCCT CATCACAGAC ATCAGTCAGA CAAGAATCCT 
GGGAGCAGTA ATTTACAGAA AATAACCAAG GAGCCAATAA TTGACTATTT TGACGTCCAG 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305687.1
CDS	157..3522
Translation	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

Target ORF information:

RefSeq Version	NM_001318758.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318758.1

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGCAGAT 
GCTACATTTA TGTGTGACAA GCGGTGTAAC AAGAAACGGT TGTGTGGACG GCATAAATGT 
AATGAGATAT GCTGTGTGGA TAAGGAGCAC AAGTGTCCTT TGATTTGTGG GAGGAAACTC 
CGTTGTGGCC TTCATAGGTG TGAAGAACCT TGTCATCGTG GAAACTGCCA GACATGCTGG 
CAAGCCAGTT TTGATGAATT AACCTGCCAT TGTGGTGCAT CAGTGATTTA CCCTCCAGTT 
CCCTGTGGTA CTAGGCCCCC TGAATGTACC CAAACCTGCG CTAGAGTCCA TGAGTGTGAC 
CATCCAGTAT ATCATTCTTG TCATAGTGAG GAGAAGTGTC CCCCTTGCAC TTTCCTAACT 
CAGAAGTGGT GCATGGGCAA GCATGAGTTT CGGAGCAACA TCCCCTGTCA CCTGGTTGAT 
ATCTCTTGCG GATTACCCTG CAGTGCCACG CTACCATGTG GGATGCACAA ATGTCAGAGA 
CTCTGTCACA AAGGGGAGTG TCTTGTGGAT GAGCCCTGCA AGCAGCCCTG CACCACCCCC 
AGAGCTGACT GTGGTCACCC GTGTATGGCA CCCTGCCATA CCAGCTCACC CTGCCCTGTG 
ACTGCTTGTA AAGCTAAGGT AGAGCTACAG TGTGAATGTG GACGAAGAAA AGAGATGGTG 
ATTTGCTCTG AAGCATCTAG TACTTATCAA AGAATAGCTG CAATCTCCAT GGCCTCTAAG 
ATAACAGACA TGCAGCTTGG AGGTTCAGTG GAGATCAGCA AGTTAATTAC CAAAAAGGAA 
GTTCATCAAG CCAGGCTGGA GTGTGATGAG GAGTGTTCAG CCTTGGAAAG GAAAAAGAGA 
TTAGCAGAGG CATTTCATAT CAGTGAGGAT TCTGATCCTT TCAATATACG TTCTTCAGGG 
TCAAAATTCA GTGATAGTTT GAAAGAAGAT GCCAGGAAGG ACTTAAAGTT TGTCAGTGAC 
GTTGAGAAGG AAATGGAAAC CCTCGTGGAG GCCGTGAATA AGGGAAAGAA TAGTAAGAAA 
AGCCACAGCT TCCCTCCCAT GAACAGAGAC CACCGCCGGA TCATCCATGA CTTGGCCCAA 
GTTTATGGCC TGGAGAGCGT GAGCTATGAC AGTGAACCGA AGCGCAATGT GGTGGTCACT 
GCCATCAGGG GGAAGTCCGT TTGTCCTCCT ACCACGCTGA CAGGTGTGCT TGAAAGGGAA 
ATGCAGGCAC GGCCTCCACC ACCGATTCCT CATCACAGAC ATCAGTCAGA CAAGAATCCT 
GGGAGCAGTA ATTTACAGAA AATAACCAAG GAGCCAATAA TTGACTATTT TGACGTCCAG 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_001318758.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001305687.1 Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu42738
Clone ID Related Accession (Same CDS sequence)	NM_001318758.1 , NM_001318758.2
Accession Version	NM_001318758.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-27
Organism	Homo sapiens(human)
Product	transcriptional repressor NF-X1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA290058.1, AL832342.1, AL356472.17, AL356218.22 and AI884552.1. On Jun 1, 2019 this sequence version replaced NM_001318758.1. Transcript Variant: This variant (4) uses an alternate in-frame splice site in an internal exon, compared to variant 1. It encodes isoform 4 which is longer than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL832342.1, SRR1803614.134139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGCAGAT 
GCTACATTTA TGTGTGACAA GCGGTGTAAC AAGAAACGGT TGTGTGGACG GCATAAATGT 
AATGAGATAT GCTGTGTGGA TAAGGAGCAC AAGTGTCCTT TGATTTGTGG GAGGAAACTC 
CGTTGTGGCC TTCATAGGTG TGAAGAACCT TGTCATCGTG GAAACTGCCA GACATGCTGG 
CAAGCCAGTT TTGATGAATT AACCTGCCAT TGTGGTGCAT CAGTGATTTA CCCTCCAGTT 
CCCTGTGGTA CTAGGCCCCC TGAATGTACC CAAACCTGCG CTAGAGTCCA TGAGTGTGAC 
CATCCAGTAT ATCATTCTTG TCATAGTGAG GAGAAGTGTC CCCCTTGCAC TTTCCTAACT 
CAGAAGTGGT GCATGGGCAA GCATGAGTTT CGGAGCAACA TCCCCTGTCA CCTGGTTGAT 
ATCTCTTGCG GATTACCCTG CAGTGCCACG CTACCATGTG GGATGCACAA ATGTCAGAGA 
CTCTGTCACA AAGGGGAGTG TCTTGTGGAT GAGCCCTGCA AGCAGCCCTG CACCACCCCC 
AGAGCTGACT GTGGTCACCC GTGTATGGCA CCCTGCCATA CCAGCTCACC CTGCCCTGTG 
ACTGCTTGTA AAGCTAAGGT AGAGCTACAG TGTGAATGTG GACGAAGAAA AGAGATGGTG 
ATTTGCTCTG AAGCATCTAG TACTTATCAA AGAATAGCTG CAATCTCCAT GGCCTCTAAG 
ATAACAGACA TGCAGCTTGG AGGTTCAGTG GAGATCAGCA AGTTAATTAC CAAAAAGGAA 
GTTCATCAAG CCAGGCTGGA GTGTGATGAG GAGTGTTCAG CCTTGGAAAG GAAAAAGAGA 
TTAGCAGAGG CATTTCATAT CAGTGAGGAT TCTGATCCTT TCAATATACG TTCTTCAGGG 
TCAAAATTCA GTGATAGTTT GAAAGAAGAT GCCAGGAAGG ACTTAAAGTT TGTCAGTGAC 
GTTGAGAAGG AAATGGAAAC CCTCGTGGAG GCCGTGAATA AGGGAAAGAA TAGTAAGAAA 
AGCCACAGCT TCCCTCCCAT GAACAGAGAC CACCGCCGGA TCATCCATGA CTTGGCCCAA 
GTTTATGGCC TGGAGAGCGT GAGCTATGAC AGTGAACCGA AGCGCAATGT GGTGGTCACT 
GCCATCAGGG GGAAGTCCGT TTGTCCTCCT ACCACGCTGA CAGGTGTGCT TGAAAGGGAA 
ATGCAGGCAC GGCCTCCACC ACCGATTCCT CATCACAGAC ATCAGTCAGA CAAGAATCCT 
GGGAGCAGTA ATTTACAGAA AATAACCAAG GAGCCAATAA TTGACTATTT TGACGTCCAG 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001305687.1
CDS	58..3423
Translation	MAEAPPVSGTFKFNTDAAEFIPQEKKNSGLNCGTQRRLDSNRIGRRNYSSPPPCHLSRQVPYDEISAVHQHSYHPSGSKPKSQQTSFQSSPCNKSPKSHGLQNQPWQKLRNEKHHIRVKKAQSLAEQTSDTAGLESSTRSESGTDLREHSPSESEKEVVGADPRGAKPKKATQFVYSYGRGPKVKGKLKCEWSNRTTPKPEDAGPESTKPVGVFHPDSSEASSRKGVLDGYGARRNEQRRYPQKRPPWEVEGARPRPGRNPPKQEGHRHTNAGHRNNMGPIPKDDLNERPAKSTCDSENLAVINKSSRRVDQEKCTVRRQDPQVVSPFSRGKQNHVLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCENILNCGQHQCAELCHGGQCQPCQIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGNHTCSQVCHPQPCQQCPRLPQLVRCCPCGQTPLSQLLELGSSSRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEFRSNIPCHLVDISCGLPCSATLPCGMHKCQRLCHKGECLVDEPCKQPCTTPRADCGHPCMAPCHTSSPCPVTACKAKVELQCECGRRKEMVICSEASSTYQRIAAISMASKITDMQLGGSVEISKLITKKEVHQARLECDEECSALERKKRLAEAFHISEDSDPFNIRSSGSKFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREMQARPPPPIPHHRHQSDKNPGSSNLQKITKEPIIDYFDVQD

Target ORF information:

RefSeq Version	NM_001318758.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear transcription factor, X-box binding 1 (NFX1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001318758.2

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTACT TTTAAATTCA ATACAGATGC TGCTGAATTC 
ATTCCTCAGG AGAAAAAAAA TTCTGGTCTA AATTGTGGGA CTCAAAGGAG ACTAGACTCT 
AATAGGATTG GTAGAAGAAA TTACAGTTCA CCACCTCCCT GTCACCTTTC CAGGCAGGTC 
CCTTATGATG AAATCTCTGC TGTTCATCAG CATAGTTATC ATCCGTCAGG AAGCAAACCT 
AAGAGTCAGC AGACGTCTTT CCAGTCCTCT CCTTGTAATA AATCGCCCAA GAGCCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AATGAGAAGC ACCATATCAG AGTCAAGAAA 
GCACAGAGTC TTGCTGAGCA GACCTCAGAT ACAGCTGGAT TAGAGAGCTC GACCAGATCA 
GAGAGTGGGA CAGACCTCAG AGAGCATAGT CCTTCTGAGA GTGAGAAGGA AGTTGTGGGT 
GCAGATCCCA GGGGAGCAAA ACCCAAAAAA GCAACACAGT TTGTATACAG CTATGGTAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAATGT GAATGGAGTA ACCGAACAAC TCCAAAACCG 
GAGGATGCTG GACCCGAAAG TACCAAACCT GTGGGGGTTT TCCACCCTGA CTCTTCAGAG 
GCATCCTCTA GAAAAGGAGT ATTGGATGGG TATGGAGCCA GACGAAATGA GCAGAGAAGA 
TACCCACAGA AAAGGCCTCC CTGGGAAGTG GAGGGGGCCA GGCCACGACC AGGCAGAAAT 
CCACCAAAAC AGGAGGGCCA CCGACATACA AACGCAGGAC ACAGAAACAA CATGGGCCCC 
ATTCCAAAGG ATGACCTCAA TGAAAGACCA GCAAAATCTA CCTGTGACAG TGAGAACTTG 
GCAGTCATCA ACAAGTCTTC CAGGAGGGTT GACCAAGAGA AATGCACTGT ACGGAGGCAG 
GATCCTCAAG TAGTATCTCC TTTCTCCCGA GGCAAACAGA ACCATGTGCT AAAGAATGTG 
GAAACGCACA CAGGTTCTCT AATTGAACAA CTAACAACAG AAAAATACGA GTGCATGGTG 
TGCTGTGAAT TGGTTCGTGT CACGGCCCCA GTGTGGAGTT GTCAGAGCTG TTACCATGTG 
TTTCATTTGA ACTGCATAAA GAAATGGGCA AGGTCTCCAG CATCTCAAGC AGATGGCCAG 
AGTGGTTGGA GGTGCCCTGC CTGTCAGAAT GTTTCTGCAC ATGTTCCTAA TACCTACACT 
TGTTTCTGTG GCAAGGTAAA GAATCCTGAG TGGAGCAGAA ATGAAATTCC ACATAGCTGT 
GGTGAGGTTT GTAGAAAGAA ACAGCCTGGC CAGGACTGCC CACATTCCTG TAACCTTCTC 
TGCCATCCAG GACCCTGCCC ACCCTGCCCT GCCTTTATGA CAAAAACATG TGAATGTGGA 
CGAACCAGGC ACACAGTTCG CTGTGGTCAG GCTGTCTCAG TCCACTGTTC TAACCCATGT 
GAGAATATTT TGAACTGTGG TCAGCACCAG TGTGCTGAGC TGTGCCATGG GGGTCAGTGC 
CAGCCTTGCC AGATCATTTT GAACCAGGTA TGCTATTGCG GCAGCACCTC CCGAGATGTG 
TTATGTGGAA CCGATGTAGG AAAGTCTGAT GGATTTGGGG ATTTCAGCTG TTTAAAGATA 
TGTGGCAAGG ACTTGAAATG CGGTAACCAT ACATGTTCGC AAGTGTGCCA CCCTCAGCCC 
TGCCAGCAAT GCCCACGGCT CCCCCAGCTG GTGCGCTGTT GCCCCTGTGG CCAAACTCCT 
CTCAGCCAAT TGCTAGAACT TGGAAGTAGT AGTCGGAAAA CATGCATGGA CCCTGTGCCT 
TCATGTGGAA AAGTGTGCGG CAAGCCTCTG CCTTGTGGTT CCTTAGATTT CATTCATACC 
TGTGAAAAGC TCTGCCATGA AGGAGACTGT GGACCATGCT CTCGCACATC AGTTATTTCC 
TGCAGATGCT CTTTCAGAAC AAAGGAGCTT CCATGTACCA GTCTCAAAAG TGAAGCAGAT 
GCTACATTTA TGTGTGACAA GCGGTGTAAC AAGAAACGGT TGTGTGGACG GCATAAATGT 
AATGAGATAT GCTGTGTGGA TAAGGAGCAC AAGTGTCCTT TGATTTGTGG GAGGAAACTC 
CGTTGTGGCC TTCATAGGTG TGAAGAACCT TGTCATCGTG GAAACTGCCA GACATGCTGG 
CAAGCCAGTT TTGATGAATT AACCTGCCAT TGTGGTGCAT CAGTGATTTA CCCTCCAGTT 
CCCTGTGGTA CTAGGCCCCC TGAATGTACC CAAACCTGCG CTAGAGTCCA TGAGTGTGAC 
CATCCAGTAT ATCATTCTTG TCATAGTGAG GAGAAGTGTC CCCCTTGCAC TTTCCTAACT 
CAGAAGTGGT GCATGGGCAA GCATGAGTTT CGGAGCAACA TCCCCTGTCA CCTGGTTGAT 
ATCTCTTGCG GATTACCCTG CAGTGCCACG CTACCATGTG GGATGCACAA ATGTCAGAGA 
CTCTGTCACA AAGGGGAGTG TCTTGTGGAT GAGCCCTGCA AGCAGCCCTG CACCACCCCC 
AGAGCTGACT GTGGTCACCC GTGTATGGCA CCCTGCCATA CCAGCTCACC CTGCCCTGTG 
ACTGCTTGTA AAGCTAAGGT AGAGCTACAG TGTGAATGTG GACGAAGAAA AGAGATGGTG 
ATTTGCTCTG AAGCATCTAG TACTTATCAA AGAATAGCTG CAATCTCCAT GGCCTCTAAG 
ATAACAGACA TGCAGCTTGG AGGTTCAGTG GAGATCAGCA AGTTAATTAC CAAAAAGGAA 
GTTCATCAAG CCAGGCTGGA GTGTGATGAG GAGTGTTCAG CCTTGGAAAG GAAAAAGAGA 
TTAGCAGAGG CATTTCATAT CAGTGAGGAT TCTGATCCTT TCAATATACG TTCTTCAGGG 
TCAAAATTCA GTGATAGTTT GAAAGAAGAT GCCAGGAAGG ACTTAAAGTT TGTCAGTGAC 
GTTGAGAAGG AAATGGAAAC CCTCGTGGAG GCCGTGAATA AGGGAAAGAA TAGTAAGAAA 
AGCCACAGCT TCCCTCCCAT GAACAGAGAC CACCGCCGGA TCATCCATGA CTTGGCCCAA 
GTTTATGGCC TGGAGAGCGT GAGCTATGAC AGTGAACCGA AGCGCAATGT GGTGGTCACT 
GCCATCAGGG GGAAGTCCGT TTGTCCTCCT ACCACGCTGA CAGGTGTGCT TGAAAGGGAA 
ATGCAGGCAC GGCCTCCACC ACCGATTCCT CATCACAGAC ATCAGTCAGA CAAGAATCCT 
GGGAGCAGTA ATTTACAGAA AATAACCAAG GAGCCAATAA TTGACTATTT TGACGTCCAG 
GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human papillomavirus type 16 E6 and NFX1-123 mislocalize immune signaling proteins and downregulate immune gene expression in keratinocytes.
PloS one12(11)e0187514(2017)
Levan J,Vliet-Gregg PA,Robinson KL,Katzenellenbogen RA

Involvement of the NFX1-repressor complex in PKC-?-induced repression of hTERT transcription.
Journal of biochemistry160(5)309-313(2016 Nov)
Yamashita S,Fujii K,Zhao C,Takagi H,Katakura Y

Human papillomavirus 16E6 and NFX1-123 potentiate Notch signaling and differentiation without activating cellular arrest.
Virology47850-60(2015 Apr)
Vliet-Gregg PA,Hamilton JR,Katzenellenbogen RA

NFX1-123 and human papillomavirus 16E6 increase Notch expression in keratinocytes.
Journal of virology87(24)13741-50(2013 Dec)
Vliet-Gregg PA,Hamilton JR,Katzenellenbogen RA

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NFX1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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