nfx1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nfx1
Entrez Gene ID	100488254
Full Name	nuclear transcription factor, X-box binding 1
General protein information	Preferred Names nuclear transcription factor, X-box binding 1 Names transcriptional repressor NF-X1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031903504.1	XP_031759364.1	transcriptional repressor NF-X1 isoform X2
XM_018094863.1	XP_017950352.1	transcriptional repressor NF-X1
XM_031903503.1	XP_031759363.1	transcriptional repressor NF-X1 isoform X1
XM_031903505.1	XP_031759365.1	transcriptional repressor NF-X1 isoform X3
XM_031903506.1	XP_031759366.1	transcriptional repressor NF-X1 isoform X4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFX1	XP_001159552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008728	XP_314840.4
Oryza sativa (rice)	Os06g0252300	NP_001057298.1
Bos taurus (cattle)	NFX1	NP_001095499.1
Rattus norvegicus (Norway rat)	Nfx1	NP_001019955.2
Drosophila melanogaster (fruit fly)	stc	NP_001260460.1
Kluyveromyces lactis	KLLA0E08317g	XP_454325.1
Xenopus tropicalis (tropical clawed frog)	nfx1	XP_002935327.2
Homo sapiens (human)	NFX1	NP_002495.2
Eremothecium gossypii	AGOS_ADL213W	NP_983883.2
Magnaporthe oryzae (rice blast fungus)	MGG_00137	XP_003718994.1
Arabidopsis thaliana (thale cress)	NFXL1	NP_172488.1
Gallus gallus (chicken)	NFX1	XP_004935125.1
Saccharomyces cerevisiae (baker's yeast)	FAP1	NP_014375.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.03	NP_588382.1
Canis lupus familiaris (dog)	NFX1	XP_005626763.1
Mus musculus (house mouse)	Nfx1	NP_076228.2
Danio rerio (zebrafish)	LOC562071	XP_690559.4
Caenorhabditis elegans (roundworm)	nfx-1	NP_498394.1

Gene Ontology

The following nfx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nfx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa59844	XM_031903506.1 Latest version!	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OXa45053	XM_018094863.1 Latest version!	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59841	XM_031903503.1 Latest version!	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59842	XM_031903504.1 Latest version!	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa59843	XM_031903505.1 Latest version!	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nfx1 ( XM_031903506.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759366.1 Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa59844
Clone ID Related Accession (Same CDS sequence)	XM_031903506.1
Accession Version	XM_031903506.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2382bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional repressor NF-X1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GTCAGCCTTC AGGCTCTCTG ATTGAACAGC TGACCGCAGA GAAATATGAA 
TGCATGGTCT GCTGTGAGGT GGTACGTGTT ATGGCCCCGG TGTGGAGCTG TCAGAGCTGC 
TACCATGTTT TCCACCTTAA CTGTATCAAG AAGTGGGCCC GGTCCCCTGC ATCACAAGCA 
GAAGGTCACA AATTGCCCAC AAGTGTCATT GATGGAAATA GCGGATGGCG ATGTCCTGCA 
TGTCAGAATG TCTCCTCTCG TGCCCCTAAT AGCTATACCT GCTTTTGCAG CAAAGTGAAC 
AACCCTGAAT GGAACAGAAA CGAGATTCCC CACAGCTGTG GTGAGCTCTG TGGGAAGAAG 
CGCTCAGGCC TGGATTGTCC TCACCTCTGC AATATTCTCT GTCACCCTGG ACCATGCCCA 
TCGTGCCCAG CATTTGTGAC AAAATCTTGT CAGTGTGGAA AAACAAGTCA GTCTGTACGC 
TGTGGGCAGT CTACCACCTT TCACTGCACA AATGTTTGTG GGAACCTGTT AAACTGTGCG 
GAACACAGCT GCACTCATGT GTGTCATGCA GGAGAATGCC AATCATGTCA GCAAATTATA 
CATCAAGCCT GCTATTGTGG TGGAAGCTCT CGTGATGTTC TTTGTGGAAC TGACAAAGAT 
AACTATGATG GCTTTGGCAA TTTCTCCTGT CAAAAGCAAT GTGGCAAGAA ATTAAATTGT 
GGAAAGCATG GCTGTACTGA AGTTTGTCAT CCTCAGCCTT GCCAACCATG TCCAAGGCTT 
CCTCAGGTTG TGCGATTCTG TCCCTGTGGG CAAACTCCCT TGAGCAAACT ATTGGAACTT 
GGATGCATAG ATCGAAAAAT CTGCACTGAC CCTATTCCTT CTTGTGGAAA GACCTGTGGA 
AAGCCTCTAC CTTGTGGCTC TCATGACTTT ATCCATACCT GTGAAAATCT TTGTCATGAA 
GGAGATTGTG GACTCTGCTC TCGTACGTCT AATATAGCTT GCCGATGCAG CTTTAAAACA 
AAGGAGGTTC CATGCTCTCT GCTACAGAAC AAAGGGGATT TTACTTTTGT ATGTGACAAG 
AGATGCAACA AAAAACGTCA GTGTGGTCGG CATAAGTGTA ATGAAATTTG CTGTGTGGAC 
ACAGATCACA AGTGCTCCTT AATTTGTGGA CATAAATTAA ATTGTGGCCT TCACAGATGC 
GATGAACCTT GTCATCGTGG AAACTGTCCA AATTGTTGGC AAACAAGCTT TGATGAGCTT 
ACGTGTTACT GTGGAGAAGC TGTGATCTTT CCTCCTATCG CTTGTGGAAC ACGACCTCCG 
GAGTGTAAGA AACCCTGCAC TCGGCAGCAT GAGTGTGATC ATCCAGTTTT TCATTCATGT 
CATAGTGAGG AGAAATGTCC CCCATGCACA TATCTTACTC AAAAATGGTG TATGGGAAAA 
CATGAGCTTA GGAACAATAT TCCATGTCAT CTTAGTGACA TTTCATGTGG TCTGCGCTGC 
AATCAACTTT TAAAATGCGA CATGCATAAA TGTAAACGCA TCTGCCACAA GGGGGAGTGC 
TTGATAAATG AAGACTGTAA ACAGCCATGT GATTTGCAGA GACCTGACTG TGGTCATCCA 
TGTATGACCC CTTGCCACCC TGCTGAGCCC TGCCCACCCT CTGTTTGCAA TGCAAAGGTA 
GATTTACAGT GTGAATGTGG ACGAAGAAAA GAGAGTGTAG TGTGCTCTGT GGCGTCCTCT 
GTTTATCAAA GGATCACAGC TATAACCATG GCTACAAAAC TTACAGAAAT GCAGCTTGGA 
GATTCTGTGG ATATTGCAAG ATTTGTCACA AAAAAAGAAA TGAAGCAAAC AAGGCTGCAG 
TGTGATGAGG AGTGCATGGC TTTGGAAAGG AACAGACGTT TGGCAGAGGC TTTGAATATT 
TCAGAAAATT CAGATCCTTT TAATACACGA TCTGGGTCAA AATATACTGA CAGCTTAAAA 
GAGGATGCCA GGAAAGACCT AAAGTTTGTC TCTGAGATTG AAAAAGAAAT TAAAGCTCTT 
GTGGATGCAG TCAACAAGGG GAAGCAAACC AAAAAAAGTC ACTGTTTCCC GCCAATGAAC 
AGAGACCATA GGAAACTTAT CCATGATTTA GCTGAGGTTT ACTGTGTAGA AAGTGTGAGC 
TATGACAGTG AGCCGAAACG CAATGTTGTA ATCACAGCTA TCAAAGGAAA ATCTATGTGC 
CCTAATGTTG CCCTTATGAC TGTGTTGGAA AGGGAGATTC AAGCAAGGCC ACCACCACCC 
ATTGCACATC ATAGGCACCA GAATGAAAGA AGCAACGGTA ATTATGTAAG AAGACTTTCA 
AGAGATGAAC CATCAATAGA CTACTTTGAT GTTCAAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759366.1
CDS	218..2599
Translation	MAEGQPSGSLIEQLTAEKYECMVCCEVVRVMAPVWSCQSCYHVFHLNCIKKWARSPASQAEGHKLPTSVIDGNSGWRCPACQNVSSRAPNSYTCFCSKVNNPEWNRNEIPHSCGELCGKKRSGLDCPHLCNILCHPGPCPSCPAFVTKSCQCGKTSQSVRCGQSTTFHCTNVCGNLLNCAEHSCTHVCHAGECQSCQQIIHQACYCGGSSRDVLCGTDKDNYDGFGNFSCQKQCGKKLNCGKHGCTEVCHPQPCQPCPRLPQVVRFCPCGQTPLSKLLELGCIDRKICTDPIPSCGKTCGKPLPCGSHDFIHTCENLCHEGDCGLCSRTSNIACRCSFKTKEVPCSLLQNKGDFTFVCDKRCNKKRQCGRHKCNEICCVDTDHKCSLICGHKLNCGLHRCDEPCHRGNCPNCWQTSFDELTCYCGEAVIFPPIACGTRPPECKKPCTRQHECDHPVFHSCHSEEKCPPCTYLTQKWCMGKHELRNNIPCHLSDISCGLRCNQLLKCDMHKCKRICHKGECLINEDCKQPCDLQRPDCGHPCMTPCHPAEPCPPSVCNAKVDLQCECGRRKESVVCSVASSVYQRITAITMATKLTEMQLGDSVDIARFVTKKEMKQTRLQCDEECMALERNRRLAEALNISENSDPFNTRSGSKYTDSLKEDARKDLKFVSEIEKEIKALVDAVNKGKQTKKSHCFPPMNRDHRKLIHDLAEVYCVESVSYDSEPKRNVVITAIKGKSMCPNVALMTVLEREIQARPPPPIAHHRHQNERSNGNYVRRLSRDEPSIDYFDVQD

Target ORF information:

RefSeq Version	XM_031903506.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903506.1

ORF Insert Sequence:

ATGGCTGAGG GTCAGCCTTC AGGCTCTCTG ATTGAACAGC TGACCGCAGA GAAATATGAA 
TGCATGGTCT GCTGTGAGGT GGTACGTGTT ATGGCCCCGG TGTGGAGCTG TCAGAGCTGC 
TACCATGTTT TCCACCTTAA CTGTATCAAG AAGTGGGCCC GGTCCCCTGC ATCACAAGCA 
GAAGGTCACA AATTGCCCAC AAGTGTCATT GATGGAAATA GCGGATGGCG ATGTCCTGCA 
TGTCAGAATG TCTCCTCTCG TGCCCCTAAT AGCTATACCT GCTTTTGCAG CAAAGTGAAC 
AACCCTGAAT GGAACAGAAA CGAGATTCCC CACAGCTGTG GTGAGCTCTG TGGGAAGAAG 
CGCTCAGGCC TGGATTGTCC TCACCTCTGC AATATTCTCT GTCACCCTGG ACCATGCCCA 
TCGTGCCCAG CATTTGTGAC AAAATCTTGT CAGTGTGGAA AAACAAGTCA GTCTGTACGC 
TGTGGGCAGT CTACCACCTT TCACTGCACA AATGTTTGTG GGAACCTGTT AAACTGTGCG 
GAACACAGCT GCACTCATGT GTGTCATGCA GGAGAATGCC AATCATGTCA GCAAATTATA 
CATCAAGCCT GCTATTGTGG TGGAAGCTCT CGTGATGTTC TTTGTGGAAC TGACAAAGAT 
AACTATGATG GCTTTGGCAA TTTCTCCTGT CAAAAGCAAT GTGGCAAGAA ATTAAATTGT 
GGAAAGCATG GCTGTACTGA AGTTTGTCAT CCTCAGCCTT GCCAACCATG TCCAAGGCTT 
CCTCAGGTTG TGCGATTCTG TCCCTGTGGG CAAACTCCCT TGAGCAAACT ATTGGAACTT 
GGATGCATAG ATCGAAAAAT CTGCACTGAC CCTATTCCTT CTTGTGGAAA GACCTGTGGA 
AAGCCTCTAC CTTGTGGCTC TCATGACTTT ATCCATACCT GTGAAAATCT TTGTCATGAA 
GGAGATTGTG GACTCTGCTC TCGTACGTCT AATATAGCTT GCCGATGCAG CTTTAAAACA 
AAGGAGGTTC CATGCTCTCT GCTACAGAAC AAAGGGGATT TTACTTTTGT ATGTGACAAG 
AGATGCAACA AAAAACGTCA GTGTGGTCGG CATAAGTGTA ATGAAATTTG CTGTGTGGAC 
ACAGATCACA AGTGCTCCTT AATTTGTGGA CATAAATTAA ATTGTGGCCT TCACAGATGC 
GATGAACCTT GTCATCGTGG AAACTGTCCA AATTGTTGGC AAACAAGCTT TGATGAGCTT 
ACGTGTTACT GTGGAGAAGC TGTGATCTTT CCTCCTATCG CTTGTGGAAC ACGACCTCCG 
GAGTGTAAGA AACCCTGCAC TCGGCAGCAT GAGTGTGATC ATCCAGTTTT TCATTCATGT 
CATAGTGAGG AGAAATGTCC CCCATGCACA TATCTTACTC AAAAATGGTG TATGGGAAAA 
CATGAGCTTA GGAACAATAT TCCATGTCAT CTTAGTGACA TTTCATGTGG TCTGCGCTGC 
AATCAACTTT TAAAATGCGA CATGCATAAA TGTAAACGCA TCTGCCACAA GGGGGAGTGC 
TTGATAAATG AAGACTGTAA ACAGCCATGT GATTTGCAGA GACCTGACTG TGGTCATCCA 
TGTATGACCC CTTGCCACCC TGCTGAGCCC TGCCCACCCT CTGTTTGCAA TGCAAAGGTA 
GATTTACAGT GTGAATGTGG ACGAAGAAAA GAGAGTGTAG TGTGCTCTGT GGCGTCCTCT 
GTTTATCAAA GGATCACAGC TATAACCATG GCTACAAAAC TTACAGAAAT GCAGCTTGGA 
GATTCTGTGG ATATTGCAAG ATTTGTCACA AAAAAAGAAA TGAAGCAAAC AAGGCTGCAG 
TGTGATGAGG AGTGCATGGC TTTGGAAAGG AACAGACGTT TGGCAGAGGC TTTGAATATT 
TCAGAAAATT CAGATCCTTT TAATACACGA TCTGGGTCAA AATATACTGA CAGCTTAAAA 
GAGGATGCCA GGAAAGACCT AAAGTTTGTC TCTGAGATTG AAAAAGAAAT TAAAGCTCTT 
GTGGATGCAG TCAACAAGGG GAAGCAAACC AAAAAAAGTC ACTGTTTCCC GCCAATGAAC 
AGAGACCATA GGAAACTTAT CCATGATTTA GCTGAGGTTT ACTGTGTAGA AAGTGTGAGC 
TATGACAGTG AGCCGAAACG CAATGTTGTA ATCACAGCTA TCAAAGGAAA ATCTATGTGC 
CCTAATGTTG CCCTTATGAC TGTGTTGGAA AGGGAGATTC AAGCAAGGCC ACCACCACCC 
ATTGCACATC ATAGGCACCA GAATGAAAGA AGCAACGGTA ATTATGTAAG AAGACTTTCA 
AGAGATGAAC CATCAATAGA CTACTTTGAT GTTCAAGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfx1 ( XM_018094863.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017950352.1 Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), mRNA.

Price & Availability↑

CloneID	OXa45053
Clone ID Related Accession (Same CDS sequence)	XM_018094863.1
Accession Version	XM_018094863.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3381bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional repressor NF-X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGAT 
GGAAATAGCG GATGGCGATG TCCTGCATGT CAGAATGTCT CCTCTCGTGC CCCTAATAGC 
TATACCTGCT TTTGCAGCAA AGTGAACAAC CCTGAATGGA ACAGAAACGA GATTCCCCAC 
AGCTGTGGTG AGCTCTGTGG GAAGAAGCGC TCAGGCCTGG ATTGTCCTCA CCTCTGCAAT 
ATTCTCTGTC ACCCTGGACC ATGCCCATCG TGCCCAGCAT TTGTGACAAA ATCTTGTCAG 
TGTGGAAAAA CAAGTCAGTC TGTACGCTGT GGGCAGTCTA CCACCTTTCA CTGCACAAAT 
GTTTGTGGGA ACCTGTTAAA CTGTGCGGAA CACAGCTGCA CTCATGTGTG TCATGCAGGA 
GAATGCCAAT CATGTCAGCA AATTATACAT CAAGCCTGCT ATTGTGGTGG AAGCTCTCGT 
GATGTTCTTT GTGGAACTGA CAAAGATAAC TATGATGGCT TTGGCAATTT CTCCTGTCAA 
AAGCAATGTG GCAAGAAATT AAATTGTGGA AAGCATGGCT GTACTGAAGT TTGTCATCCT 
CAGCCTTGCC AACCATGTCC AAGGCTTCCT CAGGTTGTGC GATTCTGTCC CTGTGGGCAA 
ACTCCCTTGA GCAAACTATT GGAACTTGGA TGCATAGATC GAAAAATCTG CACTGACCCT 
ATTCCTTCTT GTGGAAAGAC CTGTGGAAAG CCTCTACCTT GTGGCTCTCA TGGTAGATCA 
ATAGTCAATT CTTTGATGCC CAGTACAGGT ATGGGACCTG TTATCCAATT TAGAATATTT 
TGTTTTTTTG TAGGGGATTT TACTTTTGTA TGTGACAAGA GATGCAACAA AAAACGTCAG 
TGTGGTCGGC ATAAGTGTAA TGAAATTTGC TGTGTGGACA CAGATCACAA GTGCTCCTTA 
ATTTGTGGAC ATAAATTAAA TTGTGGCCTT CACAGATGCG ATGAACCTTG TCATCGTGGA 
AACTGTCCAA ATTGTTGGCA AACAAGCTTT GATGAGCTTA CGTGTTACTG TGGAGAAGCT 
GTGATCTTTC CTCCTATCGC TTGTGGAACA CGACCTCCGG AGTGTAAGAA ACCCTGCACT 
CGGCAGCATG AGTGTGATCA TCCAGTTTTT CATTCATGTC ATAGTGAGGA GAAATGTCCC 
CCATGCACAT ATCTTACTCA AAAATGGTGT ATGGGAAAAC ATGAGCTTAG GAACAATATT 
CCATGTCATC TTAGTGACAT TTCATGTGGT CTGCGCTGCA ATCAACTTTT AAAATGCGAC 
ATGCATAAAT GTAAACGCAT CTGCCACAAG GGGGAGTGCT TGATAAATGA AGACTGTAAA 
CAGCCATGTG ATTTGCAGAG ACCTGACTGT GGTCATCCAT GTATGACCCC TTGCCACCCT 
GCTGAGCCCT GCCCACCCTC TGTTTGCAAT GCAAAGGTAG ATTTACAGTG TGAATGTGGA 
CGAAGAAAAG AGAGTGTAGT GTGCTCTGTG GCGTCCTCTG TTTATCAAAG GATCACAGCT 
ATAACCATGG CTACAAAACT TACAGAAATG CAGCTTGGAG ATTCTGTGGA TATTGCAAGA 
TTTGTCACAA AAAAAGAAAT GAAGCAAACA AGGCTGCAGT GTGATGAGGA GTGCATGGCT 
TTGGAAAGGA ACAGACGTTT GGCAGAGGCT TTGAATATTT CAGAAAATTC AGATCCTTTT 
AATACACGAT CTGGGTCAAA ATATACTGAC AGCTTAAAAG AGGATGCCAG GAAAGACCTA 
AAGTTTGTCT CTGAGATTGA AAAAGAAATT AAAGCTCTTG TGGATGCAGT CAACAAGGGG 
AAGCAAACCA AAAAAAGTCA CTGTTTCCCG CCAATGAACA GAGACCATAG GAAACTTATC 
CATGATTTAG CTGAGGTTTA CTGTGTAGAA AGTGTGAGCT ATGACAGTGA GCCGAAACGC 
AATGTTGTAA TCACAGCTAT CAAAGGAAAA TCTATGTGCC CTAATGTTGC CCTTATGACT 
GTGTTGGAAA GGGAGATTCA AGCAAGGCCA CCACCACCCA TTGCACATCA TAGGCACCAG 
AATGAAAGAA GCAACGGTAA TTATGTAAGA AGACTTTCAA GAGATGAACC ATCAATAGAC 
TACTTTGATG TTCAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017950352.1
CDS	229..3609
Translation	MAEGQPSGQPKYNPDATDFIPRERQSANPNWRSHIRSEINRNWNRQSNLPTSYQSHSSRETNHDSPVQKLHPYTSSSKASERTQQGSPDTSSVKPVKNHSFQNQRWQRSRSERSHTRNHPRQPLSDASIRPFGASTPLGDWRVRTREPSDLCSSESEKEVGSADSRGAKPKRLTHVSGGSLRGPRDRIRHSYDREKRLTPKQKVEDMFENIPPQDGGKSVSSKEEIFKTRLESHDGAVGSEKKKRPQCSSKKAQHELLDWNNPRNEKAVKPLEPHGSKTSQHENTPAAAFTSKEKKTYRVKESCVSNICQEPKHHVHRWGSDGDKHSGRKQHSQTQDHMTKKSMQQGWSRPWNKNEVPKNKETHTSSLIEQLTAEKYECMVCCEVVRVMAPVWSCQSCYHVFHLNCIKKWARSPASQAEDGNSGWRCPACQNVSSRAPNSYTCFCSKVNNPEWNRNEIPHSCGELCGKKRSGLDCPHLCNILCHPGPCPSCPAFVTKSCQCGKTSQSVRCGQSTTFHCTNVCGNLLNCAEHSCTHVCHAGECQSCQQIIHQACYCGGSSRDVLCGTDKDNYDGFGNFSCQKQCGKKLNCGKHGCTEVCHPQPCQPCPRLPQVVRFCPCGQTPLSKLLELGCIDRKICTDPIPSCGKTCGKPLPCGSHGRSIVNSLMPSTGMGPVIQFRIFCFFVGDFTFVCDKRCNKKRQCGRHKCNEICCVDTDHKCSLICGHKLNCGLHRCDEPCHRGNCPNCWQTSFDELTCYCGEAVIFPPIACGTRPPECKKPCTRQHECDHPVFHSCHSEEKCPPCTYLTQKWCMGKHELRNNIPCHLSDISCGLRCNQLLKCDMHKCKRICHKGECLINEDCKQPCDLQRPDCGHPCMTPCHPAEPCPPSVCNAKVDLQCECGRRKESVVCSVASSVYQRITAITMATKLTEMQLGDSVDIARFVTKKEMKQTRLQCDEECMALERNRRLAEALNISENSDPFNTRSGSKYTDSLKEDARKDLKFVSEIEKEIKALVDAVNKGKQTKKSHCFPPMNRDHRKLIHDLAEVYCVESVSYDSEPKRNVVITAIKGKSMCPNVALMTVLEREIQARPPPPIAHHRHQNERSNGNYVRRLSRDEPSIDYFDVQD

Target ORF information:

RefSeq Version	XM_018094863.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094863.1

ORF Insert Sequence:

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGAT 
GGAAATAGCG GATGGCGATG TCCTGCATGT CAGAATGTCT CCTCTCGTGC CCCTAATAGC 
TATACCTGCT TTTGCAGCAA AGTGAACAAC CCTGAATGGA ACAGAAACGA GATTCCCCAC 
AGCTGTGGTG AGCTCTGTGG GAAGAAGCGC TCAGGCCTGG ATTGTCCTCA CCTCTGCAAT 
ATTCTCTGTC ACCCTGGACC ATGCCCATCG TGCCCAGCAT TTGTGACAAA ATCTTGTCAG 
TGTGGAAAAA CAAGTCAGTC TGTACGCTGT GGGCAGTCTA CCACCTTTCA CTGCACAAAT 
GTTTGTGGGA ACCTGTTAAA CTGTGCGGAA CACAGCTGCA CTCATGTGTG TCATGCAGGA 
GAATGCCAAT CATGTCAGCA AATTATACAT CAAGCCTGCT ATTGTGGTGG AAGCTCTCGT 
GATGTTCTTT GTGGAACTGA CAAAGATAAC TATGATGGCT TTGGCAATTT CTCCTGTCAA 
AAGCAATGTG GCAAGAAATT AAATTGTGGA AAGCATGGCT GTACTGAAGT TTGTCATCCT 
CAGCCTTGCC AACCATGTCC AAGGCTTCCT CAGGTTGTGC GATTCTGTCC CTGTGGGCAA 
ACTCCCTTGA GCAAACTATT GGAACTTGGA TGCATAGATC GAAAAATCTG CACTGACCCT 
ATTCCTTCTT GTGGAAAGAC CTGTGGAAAG CCTCTACCTT GTGGCTCTCA TGGTAGATCA 
ATAGTCAATT CTTTGATGCC CAGTACAGGT ATGGGACCTG TTATCCAATT TAGAATATTT 
TGTTTTTTTG TAGGGGATTT TACTTTTGTA TGTGACAAGA GATGCAACAA AAAACGTCAG 
TGTGGTCGGC ATAAGTGTAA TGAAATTTGC TGTGTGGACA CAGATCACAA GTGCTCCTTA 
ATTTGTGGAC ATAAATTAAA TTGTGGCCTT CACAGATGCG ATGAACCTTG TCATCGTGGA 
AACTGTCCAA ATTGTTGGCA AACAAGCTTT GATGAGCTTA CGTGTTACTG TGGAGAAGCT 
GTGATCTTTC CTCCTATCGC TTGTGGAACA CGACCTCCGG AGTGTAAGAA ACCCTGCACT 
CGGCAGCATG AGTGTGATCA TCCAGTTTTT CATTCATGTC ATAGTGAGGA GAAATGTCCC 
CCATGCACAT ATCTTACTCA AAAATGGTGT ATGGGAAAAC ATGAGCTTAG GAACAATATT 
CCATGTCATC TTAGTGACAT TTCATGTGGT CTGCGCTGCA ATCAACTTTT AAAATGCGAC 
ATGCATAAAT GTAAACGCAT CTGCCACAAG GGGGAGTGCT TGATAAATGA AGACTGTAAA 
CAGCCATGTG ATTTGCAGAG ACCTGACTGT GGTCATCCAT GTATGACCCC TTGCCACCCT 
GCTGAGCCCT GCCCACCCTC TGTTTGCAAT GCAAAGGTAG ATTTACAGTG TGAATGTGGA 
CGAAGAAAAG AGAGTGTAGT GTGCTCTGTG GCGTCCTCTG TTTATCAAAG GATCACAGCT 
ATAACCATGG CTACAAAACT TACAGAAATG CAGCTTGGAG ATTCTGTGGA TATTGCAAGA 
TTTGTCACAA AAAAAGAAAT GAAGCAAACA AGGCTGCAGT GTGATGAGGA GTGCATGGCT 
TTGGAAAGGA ACAGACGTTT GGCAGAGGCT TTGAATATTT CAGAAAATTC AGATCCTTTT 
AATACACGAT CTGGGTCAAA ATATACTGAC AGCTTAAAAG AGGATGCCAG GAAAGACCTA 
AAGTTTGTCT CTGAGATTGA AAAAGAAATT AAAGCTCTTG TGGATGCAGT CAACAAGGGG 
AAGCAAACCA AAAAAAGTCA CTGTTTCCCG CCAATGAACA GAGACCATAG GAAACTTATC 
CATGATTTAG CTGAGGTTTA CTGTGTAGAA AGTGTGAGCT ATGACAGTGA GCCGAAACGC 
AATGTTGTAA TCACAGCTAT CAAAGGAAAA TCTATGTGCC CTAATGTTGC CCTTATGACT 
GTGTTGGAAA GGGAGATTCA AGCAAGGCCA CCACCACCCA TTGCACATCA TAGGCACCAG 
AATGAAAGAA GCAACGGTAA TTATGTAAGA AGACTTTCAA GAGATGAACC ATCAATAGAC 
TACTTTGATG TTCAAGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfx1 ( XM_031903503.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759363.1 Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa59841
Clone ID Related Accession (Same CDS sequence)	XM_031903503.1
Accession Version	XM_031903503.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional repressor NF-X1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGGT 
CACAAATTGC CCACAAGTGT CATTGATGGA AATAGCGGAT GGCGATGTCC TGCATGTCAG 
AATGTCTCCT CTCGTGCCCC TAATAGCTAT ACCTGCTTTT GCAGCAAAGT GAACAACCCT 
GAATGGAACA GAAACGAGAT TCCCCACAGC TGTGGTGAGC TCTGTGGGAA GAAGCGCTCA 
GGCCTGGATT GTCCTCACCT CTGCAATATT CTCTGTCACC CTGGACCATG CCCATCGTGC 
CCAGCATTTG TGACAAAATC TTGTCAGTGT GGAAAAACAA GTCAGTCTGT ACGCTGTGGG 
CAGTCTACCA CCTTTCACTG CACAAATGTT TGTGGGAACC TGTTAAACTG TGCGGAACAC 
AGCTGCACTC ATGTGTGTCA TGCAGGAGAA TGCCAATCAT GTCAGCAAAT TATACATCAA 
GCCTGCTATT GTGGTGGAAG CTCTCGTGAT GTTCTTTGTG GAACTGACAA AGATAACTAT 
GATGGCTTTG GCAATTTCTC CTGTCAAAAG CAATGTGGCA AGAAATTAAA TTGTGGAAAG 
CATGGCTGTA CTGAAGTTTG TCATCCTCAG CCTTGCCAAC CATGTCCAAG GCTTCCTCAG 
GTTGTGCGAT TCTGTCCCTG TGGGCAAACT CCCTTGAGCA AACTATTGGA ACTTGGATGC 
ATAGATCGAA AAATCTGCAC TGACCCTATT CCTTCTTGTG GAAAGACCTG TGGAAAGCCT 
CTACCTTGTG GCTCTCATGA CTTTATCCAT ACCTGTGAAA ATCTTTGTCA TGAAGGAGAT 
TGTGGACTCT GCTCTCGTAC GTCTAATATA GCTTGCCGAT GCAGCTTTAA AACAAAGGAG 
GTTCCATGCT CTCTGCTACA GAACAAAGGG GATTTTACTT TTGTATGTGA CAAGAGATGC 
AACAAAAAAC GTCAGTGTGG TCGGCATAAG TGTAATGAAA TTTGCTGTGT GGACACAGAT 
CACAAGTGCT CCTTAATTTG TGGACATAAA TTAAATTGTG GCCTTCACAG ATGCGATGAA 
CCTTGTCATC GTGGAAACTG TCCAAATTGT TGGCAAACAA GCTTTGATGA GCTTACGTGT 
TACTGTGGAG AAGCTGTGAT CTTTCCTCCT ATCGCTTGTG GAACACGACC TCCGGAGTGT 
AAGAAACCCT GCACTCGGCA GCATGAGTGT GATCATCCAG TTTTTCATTC ATGTCATAGT 
GAGGAGAAAT GTCCCCCATG CACATATCTT ACTCAAAAAT GGTGTATGGG AAAACATGAG 
CTTAGGAACA ATATTCCATG TCATCTTAGT GACATTTCAT GTGGTCTGCG CTGCAATCAA 
CTTTTAAAAT GCGACATGCA TAAATGTAAA CGCATCTGCC ACAAGGGGGA GTGCTTGATA 
AATGAAGACT GTAAACAGCC ATGTGATTTG CAGAGACCTG ACTGTGGTCA TCCATGTATG 
ACCCCTTGCC ACCCTGCTGA GCCCTGCCCA CCCTCTGTTT GCAATGCAAA GGTAGATTTA 
CAGTGTGAAT GTGGACGAAG AAAAGAGAGT GTAGTGTGCT CTGTGGCGTC CTCTGTTTAT 
CAAAGGATCA CAGCTATAAC CATGGCTACA AAACTTACAG AAATGCAGCT TGGAGATTCT 
GTGGATATTG CAAGATTTGT CACAAAAAAA GAAATGAAGC AAACAAGGCT GCAGTGTGAT 
GAGGAGTGCA TGGCTTTGGA AAGGAACAGA CGTTTGGCAG AGGCTTTGAA TATTTCAGAA 
AATTCAGATC CTTTTAATAC ACGATCTGGG TCAAAATATA CTGACAGCTT AAAAGAGGAT 
GCCAGGAAAG ACCTAAAGTT TGTCTCTGAG ATTGAAAAAG AAATTAAAGC TCTTGTGGAT 
GCAGTCAACA AGGGGAAGCA AACCAAAAAA AGTCACTGTT TCCCGCCAAT GAACAGAGAC 
CATAGGAAAC TTATCCATGA TTTAGCTGAG GTTTACTGTG TAGAAAGTGT GAGCTATGAC 
AGTGAGCCGA AACGCAATGT TGTAATCACA GCTATCAAAG GAAAATCTAT GTGCCCTAAT 
GTTGCCCTTA TGACTGTGTT GGAAAGGGAG ATTCAAGCAA GGCCACCACC ACCCATTGCA 
CATCATAGGC ACCAGAATGA AAGAAGCAAC GGTAATTATG TAAGAAGACT TTCAAGAGAT 
GAACCATCAA TAGACTACTT TGATGTTCAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759363.1
CDS	219..3674
Translation	MAEGQPSGQPKYNPDATDFIPRERQSANPNWRSHIRSEINRNWNRQSNLPTSYQSHSSRETNHDSPVQKLHPYTSSSKASERTQQGSPDTSSVKPVKNHSFQNQRWQRSRSERSHTRNHPRQPLSDASIRPFGASTPLGDWRVRTREPSDLCSSESEKEVGSADSRGAKPKRLTHVSGGSLRGPRDRIRHSYDREKRLTPKQKVEDMFENIPPQDGGKSVSSKEEIFKTRLESHDGAVGSEKKKRPQCSSKKAQHELLDWNNPRNEKAVKPLEPHGSKTSQHENTPAAAFTSKEKKTYRVKESCVSNICQEPKHHVHRWGSDGDKHSGRKQHSQTQDHMTKKSMQQGWSRPWNKNEVPKNKETHTSSLIEQLTAEKYECMVCCEVVRVMAPVWSCQSCYHVFHLNCIKKWARSPASQAEGHKLPTSVIDGNSGWRCPACQNVSSRAPNSYTCFCSKVNNPEWNRNEIPHSCGELCGKKRSGLDCPHLCNILCHPGPCPSCPAFVTKSCQCGKTSQSVRCGQSTTFHCTNVCGNLLNCAEHSCTHVCHAGECQSCQQIIHQACYCGGSSRDVLCGTDKDNYDGFGNFSCQKQCGKKLNCGKHGCTEVCHPQPCQPCPRLPQVVRFCPCGQTPLSKLLELGCIDRKICTDPIPSCGKTCGKPLPCGSHDFIHTCENLCHEGDCGLCSRTSNIACRCSFKTKEVPCSLLQNKGDFTFVCDKRCNKKRQCGRHKCNEICCVDTDHKCSLICGHKLNCGLHRCDEPCHRGNCPNCWQTSFDELTCYCGEAVIFPPIACGTRPPECKKPCTRQHECDHPVFHSCHSEEKCPPCTYLTQKWCMGKHELRNNIPCHLSDISCGLRCNQLLKCDMHKCKRICHKGECLINEDCKQPCDLQRPDCGHPCMTPCHPAEPCPPSVCNAKVDLQCECGRRKESVVCSVASSVYQRITAITMATKLTEMQLGDSVDIARFVTKKEMKQTRLQCDEECMALERNRRLAEALNISENSDPFNTRSGSKYTDSLKEDARKDLKFVSEIEKEIKALVDAVNKGKQTKKSHCFPPMNRDHRKLIHDLAEVYCVESVSYDSEPKRNVVITAIKGKSMCPNVALMTVLEREIQARPPPPIAHHRHQNERSNGNYVRRLSRDEPSIDYFDVQD

Target ORF information:

RefSeq Version	XM_031903503.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903503.1

ORF Insert Sequence:

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGGT 
CACAAATTGC CCACAAGTGT CATTGATGGA AATAGCGGAT GGCGATGTCC TGCATGTCAG 
AATGTCTCCT CTCGTGCCCC TAATAGCTAT ACCTGCTTTT GCAGCAAAGT GAACAACCCT 
GAATGGAACA GAAACGAGAT TCCCCACAGC TGTGGTGAGC TCTGTGGGAA GAAGCGCTCA 
GGCCTGGATT GTCCTCACCT CTGCAATATT CTCTGTCACC CTGGACCATG CCCATCGTGC 
CCAGCATTTG TGACAAAATC TTGTCAGTGT GGAAAAACAA GTCAGTCTGT ACGCTGTGGG 
CAGTCTACCA CCTTTCACTG CACAAATGTT TGTGGGAACC TGTTAAACTG TGCGGAACAC 
AGCTGCACTC ATGTGTGTCA TGCAGGAGAA TGCCAATCAT GTCAGCAAAT TATACATCAA 
GCCTGCTATT GTGGTGGAAG CTCTCGTGAT GTTCTTTGTG GAACTGACAA AGATAACTAT 
GATGGCTTTG GCAATTTCTC CTGTCAAAAG CAATGTGGCA AGAAATTAAA TTGTGGAAAG 
CATGGCTGTA CTGAAGTTTG TCATCCTCAG CCTTGCCAAC CATGTCCAAG GCTTCCTCAG 
GTTGTGCGAT TCTGTCCCTG TGGGCAAACT CCCTTGAGCA AACTATTGGA ACTTGGATGC 
ATAGATCGAA AAATCTGCAC TGACCCTATT CCTTCTTGTG GAAAGACCTG TGGAAAGCCT 
CTACCTTGTG GCTCTCATGA CTTTATCCAT ACCTGTGAAA ATCTTTGTCA TGAAGGAGAT 
TGTGGACTCT GCTCTCGTAC GTCTAATATA GCTTGCCGAT GCAGCTTTAA AACAAAGGAG 
GTTCCATGCT CTCTGCTACA GAACAAAGGG GATTTTACTT TTGTATGTGA CAAGAGATGC 
AACAAAAAAC GTCAGTGTGG TCGGCATAAG TGTAATGAAA TTTGCTGTGT GGACACAGAT 
CACAAGTGCT CCTTAATTTG TGGACATAAA TTAAATTGTG GCCTTCACAG ATGCGATGAA 
CCTTGTCATC GTGGAAACTG TCCAAATTGT TGGCAAACAA GCTTTGATGA GCTTACGTGT 
TACTGTGGAG AAGCTGTGAT CTTTCCTCCT ATCGCTTGTG GAACACGACC TCCGGAGTGT 
AAGAAACCCT GCACTCGGCA GCATGAGTGT GATCATCCAG TTTTTCATTC ATGTCATAGT 
GAGGAGAAAT GTCCCCCATG CACATATCTT ACTCAAAAAT GGTGTATGGG AAAACATGAG 
CTTAGGAACA ATATTCCATG TCATCTTAGT GACATTTCAT GTGGTCTGCG CTGCAATCAA 
CTTTTAAAAT GCGACATGCA TAAATGTAAA CGCATCTGCC ACAAGGGGGA GTGCTTGATA 
AATGAAGACT GTAAACAGCC ATGTGATTTG CAGAGACCTG ACTGTGGTCA TCCATGTATG 
ACCCCTTGCC ACCCTGCTGA GCCCTGCCCA CCCTCTGTTT GCAATGCAAA GGTAGATTTA 
CAGTGTGAAT GTGGACGAAG AAAAGAGAGT GTAGTGTGCT CTGTGGCGTC CTCTGTTTAT 
CAAAGGATCA CAGCTATAAC CATGGCTACA AAACTTACAG AAATGCAGCT TGGAGATTCT 
GTGGATATTG CAAGATTTGT CACAAAAAAA GAAATGAAGC AAACAAGGCT GCAGTGTGAT 
GAGGAGTGCA TGGCTTTGGA AAGGAACAGA CGTTTGGCAG AGGCTTTGAA TATTTCAGAA 
AATTCAGATC CTTTTAATAC ACGATCTGGG TCAAAATATA CTGACAGCTT AAAAGAGGAT 
GCCAGGAAAG ACCTAAAGTT TGTCTCTGAG ATTGAAAAAG AAATTAAAGC TCTTGTGGAT 
GCAGTCAACA AGGGGAAGCA AACCAAAAAA AGTCACTGTT TCCCGCCAAT GAACAGAGAC 
CATAGGAAAC TTATCCATGA TTTAGCTGAG GTTTACTGTG TAGAAAGTGT GAGCTATGAC 
AGTGAGCCGA AACGCAATGT TGTAATCACA GCTATCAAAG GAAAATCTAT GTGCCCTAAT 
GTTGCCCTTA TGACTGTGTT GGAAAGGGAG ATTCAAGCAA GGCCACCACC ACCCATTGCA 
CATCATAGGC ACCAGAATGA AAGAAGCAAC GGTAATTATG TAAGAAGACT TTCAAGAGAT 
GAACCATCAA TAGACTACTT TGATGTTCAA GACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfx1 ( XM_031903504.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759364.1 Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa59842
Clone ID Related Accession (Same CDS sequence)	XM_031903504.1
Accession Version	XM_031903504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3429bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional repressor NF-X1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGAT 
GGAAATAGCG GATGGCGATG TCCTGCATGT CAGAATGTCT CCTCTCGTGC CCCTAATAGC 
TATACCTGCT TTTGCAGCAA AGTGAACAAC CCTGAATGGA ACAGAAACGA GATTCCCCAC 
AGCTGTGGTG AGCTCTGTGG GAAGAAGCGC TCAGGCCTGG ATTGTCCTCA CCTCTGCAAT 
ATTCTCTGTC ACCCTGGACC ATGCCCATCG TGCCCAGCAT TTGTGACAAA ATCTTGTCAG 
TGTGGAAAAA CAAGTCAGTC TGTACGCTGT GGGCAGTCTA CCACCTTTCA CTGCACAAAT 
GTTTGTGGGA ACCTGTTAAA CTGTGCGGAA CACAGCTGCA CTCATGTGTG TCATGCAGGA 
GAATGCCAAT CATGTCAGCA AATTATACAT CAAGCCTGCT ATTGTGGTGG AAGCTCTCGT 
GATGTTCTTT GTGGAACTGA CAAAGATAAC TATGATGGCT TTGGCAATTT CTCCTGTCAA 
AAGCAATGTG GCAAGAAATT AAATTGTGGA AAGCATGGCT GTACTGAAGT TTGTCATCCT 
CAGCCTTGCC AACCATGTCC AAGGCTTCCT CAGGTTGTGC GATTCTGTCC CTGTGGGCAA 
ACTCCCTTGA GCAAACTATT GGAACTTGGA TGCATAGATC GAAAAATCTG CACTGACCCT 
ATTCCTTCTT GTGGAAAGAC CTGTGGAAAG CCTCTACCTT GTGGCTCTCA TGACTTTATC 
CATACCTGTG AAAATCTTTG TCATGAAGGA GATTGTGGAC TCTGCTCTCG TACGTCTAAT 
ATAGCTTGCC GATGCAGCTT TAAAACAAAG GAGGTTCCAT GCTCTCTGCT ACAGAACAAA 
GGGGATTTTA CTTTTGTATG TGACAAGAGA TGCAACAAAA AACGTCAGTG TGGTCGGCAT 
AAGTGTAATG AAATTTGCTG TGTGGACACA GATCACAAGT GCTCCTTAAT TTGTGGACAT 
AAATTAAATT GTGGCCTTCA CAGATGCGAT GAACCTTGTC ATCGTGGAAA CTGTCCAAAT 
TGTTGGCAAA CAAGCTTTGA TGAGCTTACG TGTTACTGTG GAGAAGCTGT GATCTTTCCT 
CCTATCGCTT GTGGAACACG ACCTCCGGAG TGTAAGAAAC CCTGCACTCG GCAGCATGAG 
TGTGATCATC CAGTTTTTCA TTCATGTCAT AGTGAGGAGA AATGTCCCCC ATGCACATAT 
CTTACTCAAA AATGGTGTAT GGGAAAACAT GAGCTTAGGA ACAATATTCC ATGTCATCTT 
AGTGACATTT CATGTGGTCT GCGCTGCAAT CAACTTTTAA AATGCGACAT GCATAAATGT 
AAACGCATCT GCCACAAGGG GGAGTGCTTG ATAAATGAAG ACTGTAAACA GCCATGTGAT 
TTGCAGAGAC CTGACTGTGG TCATCCATGT ATGACCCCTT GCCACCCTGC TGAGCCCTGC 
CCACCCTCTG TTTGCAATGC AAAGGTAGAT TTACAGTGTG AATGTGGACG AAGAAAAGAG 
AGTGTAGTGT GCTCTGTGGC GTCCTCTGTT TATCAAAGGA TCACAGCTAT AACCATGGCT 
ACAAAACTTA CAGAAATGCA GCTTGGAGAT TCTGTGGATA TTGCAAGATT TGTCACAAAA 
AAAGAAATGA AGCAAACAAG GCTGCAGTGT GATGAGGAGT GCATGGCTTT GGAAAGGAAC 
AGACGTTTGG CAGAGGCTTT GAATATTTCA GAAAATTCAG ATCCTTTTAA TACACGATCT 
GGGTCAAAAT ATACTGACAG CTTAAAAGAG GATGCCAGGA AAGACCTAAA GTTTGTCTCT 
GAGATTGAAA AAGAAATTAA AGCTCTTGTG GATGCAGTCA ACAAGGGGAA GCAAACCAAA 
AAAAGTCACT GTTTCCCGCC AATGAACAGA GACCATAGGA AACTTATCCA TGATTTAGCT 
GAGGTTTACT GTGTAGAAAG TGTGAGCTAT GACAGTGAGC CGAAACGCAA TGTTGTAATC 
ACAGCTATCA AAGGAAAATC TATGTGCCCT AATGTTGCCC TTATGACTGT GTTGGAAAGG 
GAGATTCAAG CAAGGCCACC ACCACCCATT GCACATCATA GGCACCAGAA TGAAAGAAGC 
AACGGTAATT ATGTAAGAAG ACTTTCAAGA GATGAACCAT CAATAGACTA CTTTGATGTT 
CAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759364.1
CDS	218..3646
Translation	MAEGQPSGQPKYNPDATDFIPRERQSANPNWRSHIRSEINRNWNRQSNLPTSYQSHSSRETNHDSPVQKLHPYTSSSKASERTQQGSPDTSSVKPVKNHSFQNQRWQRSRSERSHTRNHPRQPLSDASIRPFGASTPLGDWRVRTREPSDLCSSESEKEVGSADSRGAKPKRLTHVSGGSLRGPRDRIRHSYDREKRLTPKQKVEDMFENIPPQDGGKSVSSKEEIFKTRLESHDGAVGSEKKKRPQCSSKKAQHELLDWNNPRNEKAVKPLEPHGSKTSQHENTPAAAFTSKEKKTYRVKESCVSNICQEPKHHVHRWGSDGDKHSGRKQHSQTQDHMTKKSMQQGWSRPWNKNEVPKNKETHTSSLIEQLTAEKYECMVCCEVVRVMAPVWSCQSCYHVFHLNCIKKWARSPASQAEDGNSGWRCPACQNVSSRAPNSYTCFCSKVNNPEWNRNEIPHSCGELCGKKRSGLDCPHLCNILCHPGPCPSCPAFVTKSCQCGKTSQSVRCGQSTTFHCTNVCGNLLNCAEHSCTHVCHAGECQSCQQIIHQACYCGGSSRDVLCGTDKDNYDGFGNFSCQKQCGKKLNCGKHGCTEVCHPQPCQPCPRLPQVVRFCPCGQTPLSKLLELGCIDRKICTDPIPSCGKTCGKPLPCGSHDFIHTCENLCHEGDCGLCSRTSNIACRCSFKTKEVPCSLLQNKGDFTFVCDKRCNKKRQCGRHKCNEICCVDTDHKCSLICGHKLNCGLHRCDEPCHRGNCPNCWQTSFDELTCYCGEAVIFPPIACGTRPPECKKPCTRQHECDHPVFHSCHSEEKCPPCTYLTQKWCMGKHELRNNIPCHLSDISCGLRCNQLLKCDMHKCKRICHKGECLINEDCKQPCDLQRPDCGHPCMTPCHPAEPCPPSVCNAKVDLQCECGRRKESVVCSVASSVYQRITAITMATKLTEMQLGDSVDIARFVTKKEMKQTRLQCDEECMALERNRRLAEALNISENSDPFNTRSGSKYTDSLKEDARKDLKFVSEIEKEIKALVDAVNKGKQTKKSHCFPPMNRDHRKLIHDLAEVYCVESVSYDSEPKRNVVITAIKGKSMCPNVALMTVLEREIQARPPPPIAHHRHQNERSNGNYVRRLSRDEPSIDYFDVQD

Target ORF information:

RefSeq Version	XM_031903504.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903504.1

ORF Insert Sequence:

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGAT 
GGAAATAGCG GATGGCGATG TCCTGCATGT CAGAATGTCT CCTCTCGTGC CCCTAATAGC 
TATACCTGCT TTTGCAGCAA AGTGAACAAC CCTGAATGGA ACAGAAACGA GATTCCCCAC 
AGCTGTGGTG AGCTCTGTGG GAAGAAGCGC TCAGGCCTGG ATTGTCCTCA CCTCTGCAAT 
ATTCTCTGTC ACCCTGGACC ATGCCCATCG TGCCCAGCAT TTGTGACAAA ATCTTGTCAG 
TGTGGAAAAA CAAGTCAGTC TGTACGCTGT GGGCAGTCTA CCACCTTTCA CTGCACAAAT 
GTTTGTGGGA ACCTGTTAAA CTGTGCGGAA CACAGCTGCA CTCATGTGTG TCATGCAGGA 
GAATGCCAAT CATGTCAGCA AATTATACAT CAAGCCTGCT ATTGTGGTGG AAGCTCTCGT 
GATGTTCTTT GTGGAACTGA CAAAGATAAC TATGATGGCT TTGGCAATTT CTCCTGTCAA 
AAGCAATGTG GCAAGAAATT AAATTGTGGA AAGCATGGCT GTACTGAAGT TTGTCATCCT 
CAGCCTTGCC AACCATGTCC AAGGCTTCCT CAGGTTGTGC GATTCTGTCC CTGTGGGCAA 
ACTCCCTTGA GCAAACTATT GGAACTTGGA TGCATAGATC GAAAAATCTG CACTGACCCT 
ATTCCTTCTT GTGGAAAGAC CTGTGGAAAG CCTCTACCTT GTGGCTCTCA TGACTTTATC 
CATACCTGTG AAAATCTTTG TCATGAAGGA GATTGTGGAC TCTGCTCTCG TACGTCTAAT 
ATAGCTTGCC GATGCAGCTT TAAAACAAAG GAGGTTCCAT GCTCTCTGCT ACAGAACAAA 
GGGGATTTTA CTTTTGTATG TGACAAGAGA TGCAACAAAA AACGTCAGTG TGGTCGGCAT 
AAGTGTAATG AAATTTGCTG TGTGGACACA GATCACAAGT GCTCCTTAAT TTGTGGACAT 
AAATTAAATT GTGGCCTTCA CAGATGCGAT GAACCTTGTC ATCGTGGAAA CTGTCCAAAT 
TGTTGGCAAA CAAGCTTTGA TGAGCTTACG TGTTACTGTG GAGAAGCTGT GATCTTTCCT 
CCTATCGCTT GTGGAACACG ACCTCCGGAG TGTAAGAAAC CCTGCACTCG GCAGCATGAG 
TGTGATCATC CAGTTTTTCA TTCATGTCAT AGTGAGGAGA AATGTCCCCC ATGCACATAT 
CTTACTCAAA AATGGTGTAT GGGAAAACAT GAGCTTAGGA ACAATATTCC ATGTCATCTT 
AGTGACATTT CATGTGGTCT GCGCTGCAAT CAACTTTTAA AATGCGACAT GCATAAATGT 
AAACGCATCT GCCACAAGGG GGAGTGCTTG ATAAATGAAG ACTGTAAACA GCCATGTGAT 
TTGCAGAGAC CTGACTGTGG TCATCCATGT ATGACCCCTT GCCACCCTGC TGAGCCCTGC 
CCACCCTCTG TTTGCAATGC AAAGGTAGAT TTACAGTGTG AATGTGGACG AAGAAAAGAG 
AGTGTAGTGT GCTCTGTGGC GTCCTCTGTT TATCAAAGGA TCACAGCTAT AACCATGGCT 
ACAAAACTTA CAGAAATGCA GCTTGGAGAT TCTGTGGATA TTGCAAGATT TGTCACAAAA 
AAAGAAATGA AGCAAACAAG GCTGCAGTGT GATGAGGAGT GCATGGCTTT GGAAAGGAAC 
AGACGTTTGG CAGAGGCTTT GAATATTTCA GAAAATTCAG ATCCTTTTAA TACACGATCT 
GGGTCAAAAT ATACTGACAG CTTAAAAGAG GATGCCAGGA AAGACCTAAA GTTTGTCTCT 
GAGATTGAAA AAGAAATTAA AGCTCTTGTG GATGCAGTCA ACAAGGGGAA GCAAACCAAA 
AAAAGTCACT GTTTCCCGCC AATGAACAGA GACCATAGGA AACTTATCCA TGATTTAGCT 
GAGGTTTACT GTGTAGAAAG TGTGAGCTAT GACAGTGAGC CGAAACGCAA TGTTGTAATC 
ACAGCTATCA AAGGAAAATC TATGTGCCCT AATGTTGCCC TTATGACTGT GTTGGAAAGG 
GAGATTCAAG CAAGGCCACC ACCACCCATT GCACATCATA GGCACCAGAA TGAAAGAAGC 
AACGGTAATT ATGTAAGAAG ACTTTCAAGA GATGAACCAT CAATAGACTA CTTTGATGTT 
CAAGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfx1 ( XM_031903505.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031759365.1 Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X3, mRNA.

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CloneID	OXa59843
Clone ID Related Accession (Same CDS sequence)	XM_031903505.1
Accession Version	XM_031903505.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	transcriptional repressor NF-X1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030682.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGGT 
CACAAATTGC CCACAAGTGT CATTGATGGA AATAGCGGAT GGCGATGTCC TGCATGTCAG 
AATGTCTCCT CTCGTGCCCC TAATAGCTAT ACCTGCTTTT GCAGCAAAGT GAACAACCCT 
GAATGGAACA GAAACGAGAT TCCCCACAGC TGTGGTGAGC TCTGTGGGAA GAAGCGCTCA 
GGCCTGGATT GTCCTCACCT CTGCAATATT CTCTGTCACC CTGGACCATG CCCATCGTGC 
CCAGCATTTG TGACAAAATC TTGTCAGTGT GGAAAAACAA GTCAGTCTGT ACGCTGTGGG 
CAGTCTACCA CCTTTCACTG CACAAATGTT TGTGGGAACC TGTTAAACTG TGCGGAACAC 
AGCTGCACTC ATGTGTGTCA TGCAGGAGAA TGCCAATCAT GTCAGCAAAT TATACATCAA 
GCCTGCTATT GTGGTGGAAG CTCTCGTGAT GTTCTTTGTG GAACTGACAA AGATAACTAT 
GATGGCTTTG GCAATTTCTC CTGTCAAAAG CAATGTGGCA AGAAATTAAA TTGTGGAAAG 
CATGGCTGTA CTGAAGTTTG TCATCCTCAG CCTTGCCAAC CATGTCCAAG GCTTCCTCAG 
GTTGTGCGAT TCTGTCCCTG TGGGCAAACT CCCTTGAGCA AACTATTGGA ACTTGGATGC 
ATAGATCGAA AAATCTGCAC TGACCCTATT CCTTCTTGTG GAAAGACCTG TGGAAAGCCT 
CTACCTTGTG GCTCTCATGA CTTTATCCAT ACCTGTGAAA ATCTTTGTCA TGAAGGAGAT 
TGTGGACTCT GCTCTCGTAC GTCTAATATA GCTTGCCGAT GCAGCTTTAA AACAAAGGAG 
GTTCCATGCT CTCTGCTACA GAACAAAGGG GATTTTACTT TTGTATGTGA CAAGAGATGC 
AACAAAAAAC GTCAGTGTGG TCGGCATAAG TGTAATGAAA TTTGCTGTGT GGACACAGAT 
CACAAGTGCT CCTTAATTTG TGGACATAAA TTAAATTGTG GCCTTCACAG ATGCGATGAA 
CCTTGTCATC GTGGAAACTG TCCAAATTGT TGGCAAACAA GCTTTGATGA GCTTACGTGT 
TACTGTGGAG AAGCTGTGAT CTTTCCTCCT ATCGCTTGTG GAACACGACC TCCGGAGTGT 
AAGAAACCCT GCACTCGGCA GCATGAGTGT GATCATCCAG TTTTTCATTC ATGTCATAGT 
GAGGAGAAAT GTCCCCCATG CACATATCTT ACTCAAAAAT GGTGTATGGG AAAACATGAG 
CTTAGGAACA ATATTCCATG TCATCTTAGT GACATTTCAT GTGGTCTGCG CTGCAATCAA 
CTTTTAAAAT GCGACATGCA TAAATGTAAA CGCATCTGCC ACAAGGGGGA GTGCTTGATA 
AATGAAGACT GTAAACAGCC ATGTGATTTG CAGAGACCTG ACTGTGGTCA TCCATGTATG 
ACCCCTTGCC ACCCTGCTGA GCCCTGCCCA CCCTCTGTTT GCAATGCAAA GGTAGATTTA 
CAGTGTGAAT GTGGACGAAG AAAAGAGAGT GTAGTGTGCT CTGTGGCGTC CTCTGTTTAT 
CAAAGGATCA CAGCTATAAC CATGGCTACA AAACTTACAG AAATGCAGCT TGGAGATTCT 
GTGGATATTG CAAGATTTGT CACAAAAAAA GAAATGAAGC AAACAAGGCT GCAGTGTGAT 
GAGGAGTGCA TGGCTTTGGA AAGGAACAGA CGTTTGGCAG AGGCTTTGAA TATTTCAGAA 
AATTCAGATC CTTTTAATAC ACGATCTGGG TCAAAATATA CTGACAGCTT AAAAGAGGAT 
GCCAGGTACT ATACAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031759365.1
CDS	219..3299
Translation	MAEGQPSGQPKYNPDATDFIPRERQSANPNWRSHIRSEINRNWNRQSNLPTSYQSHSSRETNHDSPVQKLHPYTSSSKASERTQQGSPDTSSVKPVKNHSFQNQRWQRSRSERSHTRNHPRQPLSDASIRPFGASTPLGDWRVRTREPSDLCSSESEKEVGSADSRGAKPKRLTHVSGGSLRGPRDRIRHSYDREKRLTPKQKVEDMFENIPPQDGGKSVSSKEEIFKTRLESHDGAVGSEKKKRPQCSSKKAQHELLDWNNPRNEKAVKPLEPHGSKTSQHENTPAAAFTSKEKKTYRVKESCVSNICQEPKHHVHRWGSDGDKHSGRKQHSQTQDHMTKKSMQQGWSRPWNKNEVPKNKETHTSSLIEQLTAEKYECMVCCEVVRVMAPVWSCQSCYHVFHLNCIKKWARSPASQAEGHKLPTSVIDGNSGWRCPACQNVSSRAPNSYTCFCSKVNNPEWNRNEIPHSCGELCGKKRSGLDCPHLCNILCHPGPCPSCPAFVTKSCQCGKTSQSVRCGQSTTFHCTNVCGNLLNCAEHSCTHVCHAGECQSCQQIIHQACYCGGSSRDVLCGTDKDNYDGFGNFSCQKQCGKKLNCGKHGCTEVCHPQPCQPCPRLPQVVRFCPCGQTPLSKLLELGCIDRKICTDPIPSCGKTCGKPLPCGSHDFIHTCENLCHEGDCGLCSRTSNIACRCSFKTKEVPCSLLQNKGDFTFVCDKRCNKKRQCGRHKCNEICCVDTDHKCSLICGHKLNCGLHRCDEPCHRGNCPNCWQTSFDELTCYCGEAVIFPPIACGTRPPECKKPCTRQHECDHPVFHSCHSEEKCPPCTYLTQKWCMGKHELRNNIPCHLSDISCGLRCNQLLKCDMHKCKRICHKGECLINEDCKQPCDLQRPDCGHPCMTPCHPAEPCPPSVCNAKVDLQCECGRRKESVVCSVASSVYQRITAITMATKLTEMQLGDSVDIARFVTKKEMKQTRLQCDEECMALERNRRLAEALNISENSDPFNTRSGSKYTDSLKEDARYYTR

Target ORF information:

RefSeq Version	XM_031903505.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis nuclear transcription factor, X-box binding 1 (nfx1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031903505.1

ORF Insert Sequence:

ATGGCTGAGG GTCAGCCTTC AGGTCAACCA AAATATAACC CTGATGCAAC TGACTTTATC 
CCCAGAGAGA GACAAAGTGC CAACCCAAAT TGGAGAAGTC ATATAAGATC AGAAATTAAC 
AGAAACTGGA ACAGACAAAG CAATCTTCCA ACTTCTTACC AAAGCCATTC TTCAAGGGAG 
ACAAACCATG ACTCTCCAGT ACAAAAACTT CACCCATACA CCAGCTCAAG CAAGGCTAGT 
GAAAGAACTC AACAGGGTTC TCCAGATACC AGCTCCGTCA AGCCTGTGAA GAACCACAGT 
TTTCAGAACC AGAGATGGCA AAGGTCAAGA AGCGAAAGGT CACATACTAG AAATCACCCA 
AGACAGCCAC TTTCTGATGC ATCTATAAGG CCCTTTGGTG CCAGCACACC ACTTGGGGAC 
TGGCGCGTAA GAACAAGAGA ACCAAGTGAT TTGTGTTCAT CTGAAAGTGA AAAGGAGGTT 
GGTAGTGCAG ACAGCAGAGG AGCTAAACCA AAAAGATTGA CACATGTTTC AGGAGGCTCT 
CTAAGGGGCC CCAGAGACCG AATAAGACAT TCATATGACA GAGAGAAAAG GTTGACTCCA 
AAACAAAAAG TAGAAGATAT GTTTGAAAAC ATCCCGCCCC AGGATGGGGG AAAATCGGTA 
TCCTCTAAGG AGGAGATTTT CAAGACAAGG CTAGAAAGCC ATGATGGTGC TGTAGGAAGT 
GAAAAAAAAA AAAGGCCACA GTGCTCTTCG AAGAAGGCTC AGCATGAACT TTTGGATTGG 
AACAATCCAA GAAATGAGAA AGCAGTCAAG CCACTTGAAC CACATGGCAG CAAAACGTCT 
CAACATGAAA ATACTCCAGC TGCAGCTTTT ACATCAAAAG AAAAGAAAAC TTATAGGGTA 
AAAGAGTCCT GTGTTTCAAA TATATGCCAA GAGCCAAAGC ATCATGTTCA CCGCTGGGGC 
TCTGATGGTG ACAAACATTC AGGAAGAAAA CAGCATTCCC AAACTCAGGA CCATATGACT 
AAAAAGTCTA TGCAGCAAGG GTGGAGTAGG CCATGGAATA AGAATGAAGT ACCAAAGAAT 
AAGGAGACAC ACACAAGCTC TCTGATTGAA CAGCTGACCG CAGAGAAATA TGAATGCATG 
GTCTGCTGTG AGGTGGTACG TGTTATGGCC CCGGTGTGGA GCTGTCAGAG CTGCTACCAT 
GTTTTCCACC TTAACTGTAT CAAGAAGTGG GCCCGGTCCC CTGCATCACA AGCAGAAGGT 
CACAAATTGC CCACAAGTGT CATTGATGGA AATAGCGGAT GGCGATGTCC TGCATGTCAG 
AATGTCTCCT CTCGTGCCCC TAATAGCTAT ACCTGCTTTT GCAGCAAAGT GAACAACCCT 
GAATGGAACA GAAACGAGAT TCCCCACAGC TGTGGTGAGC TCTGTGGGAA GAAGCGCTCA 
GGCCTGGATT GTCCTCACCT CTGCAATATT CTCTGTCACC CTGGACCATG CCCATCGTGC 
CCAGCATTTG TGACAAAATC TTGTCAGTGT GGAAAAACAA GTCAGTCTGT ACGCTGTGGG 
CAGTCTACCA CCTTTCACTG CACAAATGTT TGTGGGAACC TGTTAAACTG TGCGGAACAC 
AGCTGCACTC ATGTGTGTCA TGCAGGAGAA TGCCAATCAT GTCAGCAAAT TATACATCAA 
GCCTGCTATT GTGGTGGAAG CTCTCGTGAT GTTCTTTGTG GAACTGACAA AGATAACTAT 
GATGGCTTTG GCAATTTCTC CTGTCAAAAG CAATGTGGCA AGAAATTAAA TTGTGGAAAG 
CATGGCTGTA CTGAAGTTTG TCATCCTCAG CCTTGCCAAC CATGTCCAAG GCTTCCTCAG 
GTTGTGCGAT TCTGTCCCTG TGGGCAAACT CCCTTGAGCA AACTATTGGA ACTTGGATGC 
ATAGATCGAA AAATCTGCAC TGACCCTATT CCTTCTTGTG GAAAGACCTG TGGAAAGCCT 
CTACCTTGTG GCTCTCATGA CTTTATCCAT ACCTGTGAAA ATCTTTGTCA TGAAGGAGAT 
TGTGGACTCT GCTCTCGTAC GTCTAATATA GCTTGCCGAT GCAGCTTTAA AACAAAGGAG 
GTTCCATGCT CTCTGCTACA GAACAAAGGG GATTTTACTT TTGTATGTGA CAAGAGATGC 
AACAAAAAAC GTCAGTGTGG TCGGCATAAG TGTAATGAAA TTTGCTGTGT GGACACAGAT 
CACAAGTGCT CCTTAATTTG TGGACATAAA TTAAATTGTG GCCTTCACAG ATGCGATGAA 
CCTTGTCATC GTGGAAACTG TCCAAATTGT TGGCAAACAA GCTTTGATGA GCTTACGTGT 
TACTGTGGAG AAGCTGTGAT CTTTCCTCCT ATCGCTTGTG GAACACGACC TCCGGAGTGT 
AAGAAACCCT GCACTCGGCA GCATGAGTGT GATCATCCAG TTTTTCATTC ATGTCATAGT 
GAGGAGAAAT GTCCCCCATG CACATATCTT ACTCAAAAAT GGTGTATGGG AAAACATGAG 
CTTAGGAACA ATATTCCATG TCATCTTAGT GACATTTCAT GTGGTCTGCG CTGCAATCAA 
CTTTTAAAAT GCGACATGCA TAAATGTAAA CGCATCTGCC ACAAGGGGGA GTGCTTGATA 
AATGAAGACT GTAAACAGCC ATGTGATTTG CAGAGACCTG ACTGTGGTCA TCCATGTATG 
ACCCCTTGCC ACCCTGCTGA GCCCTGCCCA CCCTCTGTTT GCAATGCAAA GGTAGATTTA 
CAGTGTGAAT GTGGACGAAG AAAAGAGAGT GTAGTGTGCT CTGTGGCGTC CTCTGTTTAT 
CAAAGGATCA CAGCTATAAC CATGGCTACA AAACTTACAG AAATGCAGCT TGGAGATTCT 
GTGGATATTG CAAGATTTGT CACAAAAAAA GAAATGAAGC AAACAAGGCT GCAGTGTGAT 
GAGGAGTGCA TGGCTTTGGA AAGGAACAGA CGTTTGGCAG AGGCTTTGAA TATTTCAGAA 
AATTCAGATC CTTTTAATAC ACGATCTGGG TCAAAATATA CTGACAGCTT AAAAGAGGAT 
GCCAGGTACT ATACAAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nfx1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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