nfx-1 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	nfx-1
Entrez Gene ID	175902
Full Name	Transcriptional repressor NF-X1 homolog
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_065993.7	NP_498394.1	Transcriptional repressor NF-X1 homolog
NM_065993.6	NP_498394.1	Transcriptional repressor NF-X1 homolog

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFX1	XP_001159552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008728	XP_314840.4
Oryza sativa (rice)	Os06g0252300	NP_001057298.1
Bos taurus (cattle)	NFX1	NP_001095499.1
Rattus norvegicus (Norway rat)	Nfx1	NP_001019955.2
Drosophila melanogaster (fruit fly)	stc	NP_001260460.1
Kluyveromyces lactis	KLLA0E08317g	XP_454325.1
Xenopus tropicalis (tropical clawed frog)	nfx1	XP_002935327.2
Homo sapiens (human)	NFX1	NP_002495.2
Eremothecium gossypii	AGOS_ADL213W	NP_983883.2
Magnaporthe oryzae (rice blast fungus)	MGG_00137	XP_003718994.1
Arabidopsis thaliana (thale cress)	NFXL1	NP_172488.1
Gallus gallus (chicken)	NFX1	XP_004935125.1
Saccharomyces cerevisiae (baker's yeast)	FAP1	NP_014375.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.03	NP_588382.1
Canis lupus familiaris (dog)	NFX1	XP_005626763.1
Mus musculus (house mouse)	Nfx1	NP_076228.2
Danio rerio (zebrafish)	LOC562071	XP_690559.4
Caenorhabditis elegans (roundworm)	nfx-1	NP_498394.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following nfx-1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nfx-1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa340939	NM_065993.7 Latest version!	Caenorhabditis elegans Transcriptional repressor NF-X1 homolog (nfx-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa340939	NM_065993.6	Caenorhabditis elegans Transcriptional repressor NF-X1 homolog (nfx-1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nfx-1 ( NM_065993.7 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498394.1 Caenorhabditis elegans Transcriptional repressor NF-X1 homolog (nfx-1), mRNA.

Price & Availability↑

CloneID	OCa340939
Clone ID Related Accession (Same CDS sequence)	NM_065993.7 , NM_065993.6
Accession Version	NM_065993.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Transcriptional repressor NF-X1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Sep 4, 2019 this sequence version replaced NM_065993.6.

ATGGCCGATA CTGAAGGAAC ATCATCTAGT ATTCCAACAT CCACAAATTC TTCCCGTCAC 
CGAGCAAGTC GAGGAAGAGG AGGTCGTTTC CGTCAAGCTC CTCGACAAGT AGGAGCCAAT 
ACCGCAGATA ACCTAGATGC GGCGCCTTTG ATGAACCCGA GAGGAGGACA TCGTGGTGGT 
AGTGGAAACA AACGTGGCAG AGGACAAAAA GAAGCTACCT CTGCAAGCCG TTTACAAATG 
CAGCCAGAAG CTAATTTCAC CTTCAACCCC AATGCTGCGA CATTCAACCC AGCACAGTCG 
GTTCTCTTCG ATCCATCTGT GCCTCCACCG ACGATTGGTG CCTCGACACA TTCGAATCAA 
AATTCCAGAC AACAGGAGCC ATCGCAAAAT AGAAGAAGAC GTGGTGGTCA GCAGAACACA 
CAGCGTCGTC AGCTCGAAAT ACAAGAACAA AGAGGCGATT CTCATCCACA AAATCAATCA 
AGACAGAACA ATAGAAATCA ACTGAATGTG GATAGGATTG CAAATCAACA AAACAAGTCA 
GTGCAAAACC CATCTCGAAA TCCTGGGAAC AGCCGAAGAG GCCAAGGAGC TCGTCGACGG 
GAACAAAAAG AAGAACCGCT GACAGAAGAA GAAACTAAAA TTTTAGCGGA TAAGCCACTC 
AGAGAGGGTC TGGTATATCT TCTGGAAAAT AATAAGTATG AATGTGCGAT TTGTTACACT 
CGAATCACAA CCAGACAAGG AGTTTGGTCC TGTAAAACCT GTTATCATAT TTTCCACATT 
TCAACTGGAT GTATTACTGA TTGGGCGAGA TCAAGCAGAG ATAAAGAAGG TGCAAACACT 
TGGAGGTGTC CAACCTGCCA GACAGAAAAC GAAACCATGC CTTACAATTA TTATTGTTTC 
TGTGGGCGTA TGAGAAATCC TAATTTCCGA GTTGGAGAAG TTCCACATAG TTGCGGAGAG 
ACATGCGGTG GTGCAAGAAA GTTCGGATGT CCTCATCCGT GTACTGAACT GTGTCATCCT 
GGTCCATGCA TTGAATGCAA GCTTTTTACA ACAAAATCAT GCAATTGTGG AAAGACGAAA 
AAAAGTGTGA GATGCGGATC TGACCAAGAA GTCATGTGTG AAACAGTCTG CGGAAAGCAA 
CTCTCATGTG GCCAACACAA TTGCGAAAGA ATTTGTCATT CCGGTGATTG TGGAGAGTGT 
ACTGTAATTC TAGAACAAGA TTGTTTTTGT GGCAAAACGC CAAAAGAAGT TTCCTGCAAT 
CCGTGTGCAC ACGAGAAATA TTCATGTGGC TCAGAGTGTG ATGGAATGTT TTCGTGCGGA 
ATTCATCATT GTACGAAGAA ATGTCACGAC AAGGAGTGTG GAGAATGTGA AACAGGAGCC 
AATAGAATTC GAACGTGTCC ATGTGGAAGG AACACACTTC AATCACTTGG AGTCACTAGG 
AAAAAATGTA CAGACATGGT TCCAACTTGT GATTCAGTTT GTGACAAATG GCTAACTTGT 
GGAACTCCTG GAAAGAATCA TCATTGTCGA GAAAAGTGCC ACGAAGGTCC TTGCCCTCCA 
TGCAATCTCA ATACATCCGT AATTTGTCGC TGTGGAACGT CTAAAGGCGT CATTCCATGT 
GACGAATATT TGCAAATAAT GAAAACCACT GGTGAATATC TCTGCACAAA AAGATGTCGA 
AAAAAGAAGT CATGTGGAAT GCACAAATGC CAAGAAGTTT GCTGTATTCA AGATGAACAT 
TTTTGTCTGC AAATGTGCAA CAAACGACTT TCTTGCGGAA TTCACACATG TGAGAATGTA 
TGTCACGCTG GACAATGCCG TCCGTGTCTG CAAGCCAGTT TTGATGAGCA ATTTTGTCAT 
TGTGGTCACA CAGTTCGAAT GCCTCCGATT CCATGTGGAG CTCGTCTACC AGTGTGCTCC 
CAACCATGTG TCCGTCCACA CAGTTGTGAT CATTCTGTCT CTCATAAGTG CCATGGTGAA 
CAAAACTGTC CTCCATGCAC ACAGCTCACC GAAAAAATGT GTTATGGTGG ACATAGAGTT 
CGCAAAAATA TACCGTGTCA TATCGACTCA GTTTCATGTG GCGTGGTTTG CAAAAAACCC 
CTCAAATGTG GAGTTCATGT GTGCCAAAGA ACATGTCACG GTGAAGAATG TGAGAAAGAA 
GGGGAGAAAT GCACGAAAAA ATGTGAAACC ATCCGAGAGC TTTGCGAACA TCCTTGCGCA 
CTTCCATGTC ACGAAGATTC TCCGTGTGAG CCAAGCCCAT GCAAAGCAAG TGTGAGAATT 
TCTTGTGAAT GTGGACGAAT TAAAAAGGAC GCACCATGCT GTGAAGTTGA TAAAATGATT 
CTATCAAAAC TCGAAAAGGA AGAAGAAGAA AAATCTGAAT CGGATGGTGA AGAGAAAGTG 
GTCAAAGAAG GAATTCTCAA GCGCTCAACA AGTTTTTCTC AACTTAACTG CATGAAATGT 
GATGACGAAT GCAAGAAATT GGAAAGAAAT CGAAAAGTTG CTGAAGCTCT TGAAGTTGAT 
ACAGATGAAT ATGGAATGAA CAAACTCGCT CCAACTATTT CCTTCCCTTG TTATCTGAAA 
GAGATGGTTA GAACCAACAT TGATTTCGTC AAATCTGTTG AAAAGATTCT CATTGATCTG 
GTTATTCAAA TTCTAAGTGG TGAAGCCTAC CACGACACTT TTCGAGCTCA CCTGCCAGCA 
ATGAGTATTG AGAAGAGAAG ATTCGTGCAT GAATACGCCA ACTTTTTCAA TATCGCCTCA 
GAAAGTGTAG ACAGTCCACC AAAAAGAAGT ATCGTTCTCA CAGCAGTACG AGGAAAATCT 
CATCAGCCTC TCGTTCTCAT TAGTGATCTT GTAAACTACA AAGGAGCTCT AAAGACGCCA 
GGACCTGCTG TTATTAGAAA AGATATAATG GATCAAGCTT TGAGTAAAAA AGAAGAAACA 
GAAGGTCTCA TGAAACCATT AAGATGTACG GAAAAAATGG TAGTTCGGAG AGAAGCTCGT 
CCAATGAAGG AAATAACTGC CCCAATCCCA TTGAAACAAC AAAACCAATT TGCTCTTTTG 
GGGAGCGATG TCGATTCGGA CGATGAAGAA AGTAACGTTC CGACAACAAG TAATTTAGTA 
TCTTCACCTC CAAAAGATTG GTGGAAAGAC GAGGAAGAAG GGTGGCAAAA AGTGCAACAA 
AAAGAGTATG TTGTTGAAGT CGAACGCGAT ATGACCGAAG ATGAGATTGA GGCGGCGAAA 
AAACTGGATG AAGGTCCCAC ATGGGAAGAT CAATGTGACG AAGATGCACC AGCAACAGAA 
ACCAGTTCAG CTATTGAATC TCCAGCGAAA GAGGAGCCGG TTGCTGAATT ACTAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498394.1
CDS	13..3372
Translation	MADTEGTSSSIPTSTNSSRHRASRGRGGRFRQAPRQVGANTADNLDAAPLMNPRGGHRGGSGNKRGRGQKEATSASRLQMQPEANFTFNPNAATFNPAQSVLFDPSVPPPTIGASTHSNQNSRQQEPSQNRRRRGGQQNTQRRQLEIQEQRGDSHPQNQSRQNNRNQLNVDRIANQQNKSVQNPSRNPGNSRRGQGARRREQKEEPLTEEETKILADKPLREGLVYLLENNKYECAICYTRITTRQGVWSCKTCYHIFHISTGCITDWARSSRDKEGANTWRCPTCQTENETMPYNYYCFCGRMRNPNFRVGEVPHSCGETCGGARKFGCPHPCTELCHPGPCIECKLFTTKSCNCGKTKKSVRCGSDQEVMCETVCGKQLSCGQHNCERICHSGDCGECTVILEQDCFCGKTPKEVSCNPCAHEKYSCGSECDGMFSCGIHHCTKKCHDKECGECETGANRIRTCPCGRNTLQSLGVTRKKCTDMVPTCDSVCDKWLTCGTPGKNHHCREKCHEGPCPPCNLNTSVICRCGTSKGVIPCDEYLQIMKTTGEYLCTKRCRKKKSCGMHKCQEVCCIQDEHFCLQMCNKRLSCGIHTCENVCHAGQCRPCLQASFDEQFCHCGHTVRMPPIPCGARLPVCSQPCVRPHSCDHSVSHKCHGEQNCPPCTQLTEKMCYGGHRVRKNIPCHIDSVSCGVVCKKPLKCGVHVCQRTCHGEECEKEGEKCTKKCETIRELCEHPCALPCHEDSPCEPSPCKASVRISCECGRIKKDAPCCEVDKMILSKLEKEEEEKSESDGEEKVVKEGILKRSTSFSQLNCMKCDDECKKLERNRKVAEALEVDTDEYGMNKLAPTISFPCYLKEMVRTNIDFVKSVEKILIDLVIQILSGEAYHDTFRAHLPAMSIEKRRFVHEYANFFNIASESVDSPPKRSIVLTAVRGKSHQPLVLISDLVNYKGALKTPGPAVIRKDIMDQALSKKEETEGLMKPLRCTEKMVVRREARPMKEITAPIPLKQQNQFALLGSDVDSDDEESNVPTTSNLVSSPPKDWWKDEEEGWQKVQQKEYVVEVERDMTEDEIEAAKKLDEGPTWEDQCDEDAPATETSSAIESPAKEEPVAELLE

Target ORF information:

RefSeq Version	NM_065993.7
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transcriptional repressor NF-X1 homolog (nfx-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065993.7

ORF Insert Sequence:

ATGGCCGATA CTGAAGGAAC ATCATCTAGT ATTCCAACAT CCACAAATTC TTCCCGTCAC 
CGAGCAAGTC GAGGAAGAGG AGGTCGTTTC CGTCAAGCTC CTCGACAAGT AGGAGCCAAT 
ACCGCAGATA ACCTAGATGC GGCGCCTTTG ATGAACCCGA GAGGAGGACA TCGTGGTGGT 
AGTGGAAACA AACGTGGCAG AGGACAAAAA GAAGCTACCT CTGCAAGCCG TTTACAAATG 
CAGCCAGAAG CTAATTTCAC CTTCAACCCC AATGCTGCGA CATTCAACCC AGCACAGTCG 
GTTCTCTTCG ATCCATCTGT GCCTCCACCG ACGATTGGTG CCTCGACACA TTCGAATCAA 
AATTCCAGAC AACAGGAGCC ATCGCAAAAT AGAAGAAGAC GTGGTGGTCA GCAGAACACA 
CAGCGTCGTC AGCTCGAAAT ACAAGAACAA AGAGGCGATT CTCATCCACA AAATCAATCA 
AGACAGAACA ATAGAAATCA ACTGAATGTG GATAGGATTG CAAATCAACA AAACAAGTCA 
GTGCAAAACC CATCTCGAAA TCCTGGGAAC AGCCGAAGAG GCCAAGGAGC TCGTCGACGG 
GAACAAAAAG AAGAACCGCT GACAGAAGAA GAAACTAAAA TTTTAGCGGA TAAGCCACTC 
AGAGAGGGTC TGGTATATCT TCTGGAAAAT AATAAGTATG AATGTGCGAT TTGTTACACT 
CGAATCACAA CCAGACAAGG AGTTTGGTCC TGTAAAACCT GTTATCATAT TTTCCACATT 
TCAACTGGAT GTATTACTGA TTGGGCGAGA TCAAGCAGAG ATAAAGAAGG TGCAAACACT 
TGGAGGTGTC CAACCTGCCA GACAGAAAAC GAAACCATGC CTTACAATTA TTATTGTTTC 
TGTGGGCGTA TGAGAAATCC TAATTTCCGA GTTGGAGAAG TTCCACATAG TTGCGGAGAG 
ACATGCGGTG GTGCAAGAAA GTTCGGATGT CCTCATCCGT GTACTGAACT GTGTCATCCT 
GGTCCATGCA TTGAATGCAA GCTTTTTACA ACAAAATCAT GCAATTGTGG AAAGACGAAA 
AAAAGTGTGA GATGCGGATC TGACCAAGAA GTCATGTGTG AAACAGTCTG CGGAAAGCAA 
CTCTCATGTG GCCAACACAA TTGCGAAAGA ATTTGTCATT CCGGTGATTG TGGAGAGTGT 
ACTGTAATTC TAGAACAAGA TTGTTTTTGT GGCAAAACGC CAAAAGAAGT TTCCTGCAAT 
CCGTGTGCAC ACGAGAAATA TTCATGTGGC TCAGAGTGTG ATGGAATGTT TTCGTGCGGA 
ATTCATCATT GTACGAAGAA ATGTCACGAC AAGGAGTGTG GAGAATGTGA AACAGGAGCC 
AATAGAATTC GAACGTGTCC ATGTGGAAGG AACACACTTC AATCACTTGG AGTCACTAGG 
AAAAAATGTA CAGACATGGT TCCAACTTGT GATTCAGTTT GTGACAAATG GCTAACTTGT 
GGAACTCCTG GAAAGAATCA TCATTGTCGA GAAAAGTGCC ACGAAGGTCC TTGCCCTCCA 
TGCAATCTCA ATACATCCGT AATTTGTCGC TGTGGAACGT CTAAAGGCGT CATTCCATGT 
GACGAATATT TGCAAATAAT GAAAACCACT GGTGAATATC TCTGCACAAA AAGATGTCGA 
AAAAAGAAGT CATGTGGAAT GCACAAATGC CAAGAAGTTT GCTGTATTCA AGATGAACAT 
TTTTGTCTGC AAATGTGCAA CAAACGACTT TCTTGCGGAA TTCACACATG TGAGAATGTA 
TGTCACGCTG GACAATGCCG TCCGTGTCTG CAAGCCAGTT TTGATGAGCA ATTTTGTCAT 
TGTGGTCACA CAGTTCGAAT GCCTCCGATT CCATGTGGAG CTCGTCTACC AGTGTGCTCC 
CAACCATGTG TCCGTCCACA CAGTTGTGAT CATTCTGTCT CTCATAAGTG CCATGGTGAA 
CAAAACTGTC CTCCATGCAC ACAGCTCACC GAAAAAATGT GTTATGGTGG ACATAGAGTT 
CGCAAAAATA TACCGTGTCA TATCGACTCA GTTTCATGTG GCGTGGTTTG CAAAAAACCC 
CTCAAATGTG GAGTTCATGT GTGCCAAAGA ACATGTCACG GTGAAGAATG TGAGAAAGAA 
GGGGAGAAAT GCACGAAAAA ATGTGAAACC ATCCGAGAGC TTTGCGAACA TCCTTGCGCA 
CTTCCATGTC ACGAAGATTC TCCGTGTGAG CCAAGCCCAT GCAAAGCAAG TGTGAGAATT 
TCTTGTGAAT GTGGACGAAT TAAAAAGGAC GCACCATGCT GTGAAGTTGA TAAAATGATT 
CTATCAAAAC TCGAAAAGGA AGAAGAAGAA AAATCTGAAT CGGATGGTGA AGAGAAAGTG 
GTCAAAGAAG GAATTCTCAA GCGCTCAACA AGTTTTTCTC AACTTAACTG CATGAAATGT 
GATGACGAAT GCAAGAAATT GGAAAGAAAT CGAAAAGTTG CTGAAGCTCT TGAAGTTGAT 
ACAGATGAAT ATGGAATGAA CAAACTCGCT CCAACTATTT CCTTCCCTTG TTATCTGAAA 
GAGATGGTTA GAACCAACAT TGATTTCGTC AAATCTGTTG AAAAGATTCT CATTGATCTG 
GTTATTCAAA TTCTAAGTGG TGAAGCCTAC CACGACACTT TTCGAGCTCA CCTGCCAGCA 
ATGAGTATTG AGAAGAGAAG ATTCGTGCAT GAATACGCCA ACTTTTTCAA TATCGCCTCA 
GAAAGTGTAG ACAGTCCACC AAAAAGAAGT ATCGTTCTCA CAGCAGTACG AGGAAAATCT 
CATCAGCCTC TCGTTCTCAT TAGTGATCTT GTAAACTACA AAGGAGCTCT AAAGACGCCA 
GGACCTGCTG TTATTAGAAA AGATATAATG GATCAAGCTT TGAGTAAAAA AGAAGAAACA 
GAAGGTCTCA TGAAACCATT AAGATGTACG GAAAAAATGG TAGTTCGGAG AGAAGCTCGT 
CCAATGAAGG AAATAACTGC CCCAATCCCA TTGAAACAAC AAAACCAATT TGCTCTTTTG 
GGGAGCGATG TCGATTCGGA CGATGAAGAA AGTAACGTTC CGACAACAAG TAATTTAGTA 
TCTTCACCTC CAAAAGATTG GTGGAAAGAC GAGGAAGAAG GGTGGCAAAA AGTGCAACAA 
AAAGAGTATG TTGTTGAAGT CGAACGCGAT ATGACCGAAG ATGAGATTGA GGCGGCGAAA 
AAACTGGATG AAGGTCCCAC ATGGGAAGAT CAATGTGACG AAGATGCACC AGCAACAGAA 
ACCAGTTCAG CTATTGAATC TCCAGCGAAA GAGGAGCCGG TTGCTGAATT ACTAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nfx-1 ( NM_065993.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_498394.1 Caenorhabditis elegans Transcriptional repressor NF-X1 homolog (nfx-1), partial mRNA.

Price & Availability↑

CloneID	OCa340939
Clone ID Related Accession (Same CDS sequence)	NM_065993.7 , NM_065993.6
Accession Version	NM_065993.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3360bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Transcriptional repressor NF-X1 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003281). On Jun 26, 2012 this sequence version replaced NM_065993.5. COMPLETENESS: incomplete on both ends.

ATGGCCGATA CTGAAGGAAC ATCATCTAGT ATTCCAACAT CCACAAATTC TTCCCGTCAC 
CGAGCAAGTC GAGGAAGAGG AGGTCGTTTC CGTCAAGCTC CTCGACAAGT AGGAGCCAAT 
ACCGCAGATA ACCTAGATGC GGCGCCTTTG ATGAACCCGA GAGGAGGACA TCGTGGTGGT 
AGTGGAAACA AACGTGGCAG AGGACAAAAA GAAGCTACCT CTGCAAGCCG TTTACAAATG 
CAGCCAGAAG CTAATTTCAC CTTCAACCCC AATGCTGCGA CATTCAACCC AGCACAGTCG 
GTTCTCTTCG ATCCATCTGT GCCTCCACCG ACGATTGGTG CCTCGACACA TTCGAATCAA 
AATTCCAGAC AACAGGAGCC ATCGCAAAAT AGAAGAAGAC GTGGTGGTCA GCAGAACACA 
CAGCGTCGTC AGCTCGAAAT ACAAGAACAA AGAGGCGATT CTCATCCACA AAATCAATCA 
AGACAGAACA ATAGAAATCA ACTGAATGTG GATAGGATTG CAAATCAACA AAACAAGTCA 
GTGCAAAACC CATCTCGAAA TCCTGGGAAC AGCCGAAGAG GCCAAGGAGC TCGTCGACGG 
GAACAAAAAG AAGAACCGCT GACAGAAGAA GAAACTAAAA TTTTAGCGGA TAAGCCACTC 
AGAGAGGGTC TGGTATATCT TCTGGAAAAT AATAAGTATG AATGTGCGAT TTGTTACACT 
CGAATCACAA CCAGACAAGG AGTTTGGTCC TGTAAAACCT GTTATCATAT TTTCCACATT 
TCAACTGGAT GTATTACTGA TTGGGCGAGA TCAAGCAGAG ATAAAGAAGG TGCAAACACT 
TGGAGGTGTC CAACCTGCCA GACAGAAAAC GAAACCATGC CTTACAATTA TTATTGTTTC 
TGTGGGCGTA TGAGAAATCC TAATTTCCGA GTTGGAGAAG TTCCACATAG TTGCGGAGAG 
ACATGCGGTG GTGCAAGAAA GTTCGGATGT CCTCATCCGT GTACTGAACT GTGTCATCCT 
GGTCCATGCA TTGAATGCAA GCTTTTTACA ACAAAATCAT GCAATTGTGG AAAGACGAAA 
AAAAGTGTGA GATGCGGATC TGACCAAGAA GTCATGTGTG AAACAGTCTG CGGAAAGCAA 
CTCTCATGTG GCCAACACAA TTGCGAAAGA ATTTGTCATT CCGGTGATTG TGGAGAGTGT 
ACTGTAATTC TAGAACAAGA TTGTTTTTGT GGCAAAACGC CAAAAGAAGT TTCCTGCAAT 
CCGTGTGCAC ACGAGAAATA TTCATGTGGC TCAGAGTGTG ATGGAATGTT TTCGTGCGGA 
ATTCATCATT GTACGAAGAA ATGTCACGAC AAGGAGTGTG GAGAATGTGA AACAGGAGCC 
AATAGAATTC GAACGTGTCC ATGTGGAAGG AACACACTTC AATCACTTGG AGTCACTAGG 
AAAAAATGTA CAGACATGGT TCCAACTTGT GATTCAGTTT GTGACAAATG GCTAACTTGT 
GGAACTCCTG GAAAGAATCA TCATTGTCGA GAAAAGTGCC ACGAAGGTCC TTGCCCTCCA 
TGCAATCTCA ATACATCCGT AATTTGTCGC TGTGGAACGT CTAAAGGCGT CATTCCATGT 
GACGAATATT TGCAAATAAT GAAAACCACT GGTGAATATC TCTGCACAAA AAGATGTCGA 
AAAAAGAAGT CATGTGGAAT GCACAAATGC CAAGAAGTTT GCTGTATTCA AGATGAACAT 
TTTTGTCTGC AAATGTGCAA CAAACGACTT TCTTGCGGAA TTCACACATG TGAGAATGTA 
TGTCACGCTG GACAATGCCG TCCGTGTCTG CAAGCCAGTT TTGATGAGCA ATTTTGTCAT 
TGTGGTCACA CAGTTCGAAT GCCTCCGATT CCATGTGGAG CTCGTCTACC AGTGTGCTCC 
CAACCATGTG TCCGTCCACA CAGTTGTGAT CATTCTGTCT CTCATAAGTG CCATGGTGAA 
CAAAACTGTC CTCCATGCAC ACAGCTCACC GAAAAAATGT GTTATGGTGG ACATAGAGTT 
CGCAAAAATA TACCGTGTCA TATCGACTCA GTTTCATGTG GCGTGGTTTG CAAAAAACCC 
CTCAAATGTG GAGTTCATGT GTGCCAAAGA ACATGTCACG GTGAAGAATG TGAGAAAGAA 
GGGGAGAAAT GCACGAAAAA ATGTGAAACC ATCCGAGAGC TTTGCGAACA TCCTTGCGCA 
CTTCCATGTC ACGAAGATTC TCCGTGTGAG CCAAGCCCAT GCAAAGCAAG TGTGAGAATT 
TCTTGTGAAT GTGGACGAAT TAAAAAGGAC GCACCATGCT GTGAAGTTGA TAAAATGATT 
CTATCAAAAC TCGAAAAGGA AGAAGAAGAA AAATCTGAAT CGGATGGTGA AGAGAAAGTG 
GTCAAAGAAG GAATTCTCAA GCGCTCAACA AGTTTTTCTC AACTTAACTG CATGAAATGT 
GATGACGAAT GCAAGAAATT GGAAAGAAAT CGAAAAGTTG CTGAAGCTCT TGAAGTTGAT 
ACAGATGAAT ATGGAATGAA CAAACTCGCT CCAACTATTT CCTTCCCTTG TTATCTGAAA 
GAGATGGTTA GAACCAACAT TGATTTCGTC AAATCTGTTG AAAAGATTCT CATTGATCTG 
GTTATTCAAA TTCTAAGTGG TGAAGCCTAC CACGACACTT TTCGAGCTCA CCTGCCAGCA 
ATGAGTATTG AGAAGAGAAG ATTCGTGCAT GAATACGCCA ACTTTTTCAA TATCGCCTCA 
GAAAGTGTAG ACAGTCCACC AAAAAGAAGT ATCGTTCTCA CAGCAGTACG AGGAAAATCT 
CATCAGCCTC TCGTTCTCAT TAGTGATCTT GTAAACTACA AAGGAGCTCT AAAGACGCCA 
GGACCTGCTG TTATTAGAAA AGATATAATG GATCAAGCTT TGAGTAAAAA AGAAGAAACA 
GAAGGTCTCA TGAAACCATT AAGATGTACG GAAAAAATGG TAGTTCGGAG AGAAGCTCGT 
CCAATGAAGG AAATAACTGC CCCAATCCCA TTGAAACAAC AAAACCAATT TGCTCTTTTG 
GGGAGCGATG TCGATTCGGA CGATGAAGAA AGTAACGTTC CGACAACAAG TAATTTAGTA 
TCTTCACCTC CAAAAGATTG GTGGAAAGAC GAGGAAGAAG GGTGGCAAAA AGTGCAACAA 
AAAGAGTATG TTGTTGAAGT CGAACGCGAT ATGACCGAAG ATGAGATTGA GGCGGCGAAA 
AAACTGGATG AAGGTCCCAC ATGGGAAGAT CAATGTGACG AAGATGCACC AGCAACAGAA 
ACCAGTTCAG CTATTGAATC TCCAGCGAAA GAGGAGCCGG TTGCTGAATT ACTAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_498394.1
CDS	1..3360
Translation	MADTEGTSSSIPTSTNSSRHRASRGRGGRFRQAPRQVGANTADNLDAAPLMNPRGGHRGGSGNKRGRGQKEATSASRLQMQPEANFTFNPNAATFNPAQSVLFDPSVPPPTIGASTHSNQNSRQQEPSQNRRRRGGQQNTQRRQLEIQEQRGDSHPQNQSRQNNRNQLNVDRIANQQNKSVQNPSRNPGNSRRGQGARRREQKEEPLTEEETKILADKPLREGLVYLLENNKYECAICYTRITTRQGVWSCKTCYHIFHISTGCITDWARSSRDKEGANTWRCPTCQTENETMPYNYYCFCGRMRNPNFRVGEVPHSCGETCGGARKFGCPHPCTELCHPGPCIECKLFTTKSCNCGKTKKSVRCGSDQEVMCETVCGKQLSCGQHNCERICHSGDCGECTVILEQDCFCGKTPKEVSCNPCAHEKYSCGSECDGMFSCGIHHCTKKCHDKECGECETGANRIRTCPCGRNTLQSLGVTRKKCTDMVPTCDSVCDKWLTCGTPGKNHHCREKCHEGPCPPCNLNTSVICRCGTSKGVIPCDEYLQIMKTTGEYLCTKRCRKKKSCGMHKCQEVCCIQDEHFCLQMCNKRLSCGIHTCENVCHAGQCRPCLQASFDEQFCHCGHTVRMPPIPCGARLPVCSQPCVRPHSCDHSVSHKCHGEQNCPPCTQLTEKMCYGGHRVRKNIPCHIDSVSCGVVCKKPLKCGVHVCQRTCHGEECEKEGEKCTKKCETIRELCEHPCALPCHEDSPCEPSPCKASVRISCECGRIKKDAPCCEVDKMILSKLEKEEEEKSESDGEEKVVKEGILKRSTSFSQLNCMKCDDECKKLERNRKVAEALEVDTDEYGMNKLAPTISFPCYLKEMVRTNIDFVKSVEKILIDLVIQILSGEAYHDTFRAHLPAMSIEKRRFVHEYANFFNIASESVDSPPKRSIVLTAVRGKSHQPLVLISDLVNYKGALKTPGPAVIRKDIMDQALSKKEETEGLMKPLRCTEKMVVRREARPMKEITAPIPLKQQNQFALLGSDVDSDDEESNVPTTSNLVSSPPKDWWKDEEEGWQKVQQKEYVVEVERDMTEDEIEAAKKLDEGPTWEDQCDEDAPATETSSAIESPAKEEPVAELLE

Target ORF information:

RefSeq Version	NM_065993.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Transcriptional repressor NF-X1 homolog (nfx-1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_065993.6

ORF Insert Sequence:

ATGGCCGATA CTGAAGGAAC ATCATCTAGT ATTCCAACAT CCACAAATTC TTCCCGTCAC 
CGAGCAAGTC GAGGAAGAGG AGGTCGTTTC CGTCAAGCTC CTCGACAAGT AGGAGCCAAT 
ACCGCAGATA ACCTAGATGC GGCGCCTTTG ATGAACCCGA GAGGAGGACA TCGTGGTGGT 
AGTGGAAACA AACGTGGCAG AGGACAAAAA GAAGCTACCT CTGCAAGCCG TTTACAAATG 
CAGCCAGAAG CTAATTTCAC CTTCAACCCC AATGCTGCGA CATTCAACCC AGCACAGTCG 
GTTCTCTTCG ATCCATCTGT GCCTCCACCG ACGATTGGTG CCTCGACACA TTCGAATCAA 
AATTCCAGAC AACAGGAGCC ATCGCAAAAT AGAAGAAGAC GTGGTGGTCA GCAGAACACA 
CAGCGTCGTC AGCTCGAAAT ACAAGAACAA AGAGGCGATT CTCATCCACA AAATCAATCA 
AGACAGAACA ATAGAAATCA ACTGAATGTG GATAGGATTG CAAATCAACA AAACAAGTCA 
GTGCAAAACC CATCTCGAAA TCCTGGGAAC AGCCGAAGAG GCCAAGGAGC TCGTCGACGG 
GAACAAAAAG AAGAACCGCT GACAGAAGAA GAAACTAAAA TTTTAGCGGA TAAGCCACTC 
AGAGAGGGTC TGGTATATCT TCTGGAAAAT AATAAGTATG AATGTGCGAT TTGTTACACT 
CGAATCACAA CCAGACAAGG AGTTTGGTCC TGTAAAACCT GTTATCATAT TTTCCACATT 
TCAACTGGAT GTATTACTGA TTGGGCGAGA TCAAGCAGAG ATAAAGAAGG TGCAAACACT 
TGGAGGTGTC CAACCTGCCA GACAGAAAAC GAAACCATGC CTTACAATTA TTATTGTTTC 
TGTGGGCGTA TGAGAAATCC TAATTTCCGA GTTGGAGAAG TTCCACATAG TTGCGGAGAG 
ACATGCGGTG GTGCAAGAAA GTTCGGATGT CCTCATCCGT GTACTGAACT GTGTCATCCT 
GGTCCATGCA TTGAATGCAA GCTTTTTACA ACAAAATCAT GCAATTGTGG AAAGACGAAA 
AAAAGTGTGA GATGCGGATC TGACCAAGAA GTCATGTGTG AAACAGTCTG CGGAAAGCAA 
CTCTCATGTG GCCAACACAA TTGCGAAAGA ATTTGTCATT CCGGTGATTG TGGAGAGTGT 
ACTGTAATTC TAGAACAAGA TTGTTTTTGT GGCAAAACGC CAAAAGAAGT TTCCTGCAAT 
CCGTGTGCAC ACGAGAAATA TTCATGTGGC TCAGAGTGTG ATGGAATGTT TTCGTGCGGA 
ATTCATCATT GTACGAAGAA ATGTCACGAC AAGGAGTGTG GAGAATGTGA AACAGGAGCC 
AATAGAATTC GAACGTGTCC ATGTGGAAGG AACACACTTC AATCACTTGG AGTCACTAGG 
AAAAAATGTA CAGACATGGT TCCAACTTGT GATTCAGTTT GTGACAAATG GCTAACTTGT 
GGAACTCCTG GAAAGAATCA TCATTGTCGA GAAAAGTGCC ACGAAGGTCC TTGCCCTCCA 
TGCAATCTCA ATACATCCGT AATTTGTCGC TGTGGAACGT CTAAAGGCGT CATTCCATGT 
GACGAATATT TGCAAATAAT GAAAACCACT GGTGAATATC TCTGCACAAA AAGATGTCGA 
AAAAAGAAGT CATGTGGAAT GCACAAATGC CAAGAAGTTT GCTGTATTCA AGATGAACAT 
TTTTGTCTGC AAATGTGCAA CAAACGACTT TCTTGCGGAA TTCACACATG TGAGAATGTA 
TGTCACGCTG GACAATGCCG TCCGTGTCTG CAAGCCAGTT TTGATGAGCA ATTTTGTCAT 
TGTGGTCACA CAGTTCGAAT GCCTCCGATT CCATGTGGAG CTCGTCTACC AGTGTGCTCC 
CAACCATGTG TCCGTCCACA CAGTTGTGAT CATTCTGTCT CTCATAAGTG CCATGGTGAA 
CAAAACTGTC CTCCATGCAC ACAGCTCACC GAAAAAATGT GTTATGGTGG ACATAGAGTT 
CGCAAAAATA TACCGTGTCA TATCGACTCA GTTTCATGTG GCGTGGTTTG CAAAAAACCC 
CTCAAATGTG GAGTTCATGT GTGCCAAAGA ACATGTCACG GTGAAGAATG TGAGAAAGAA 
GGGGAGAAAT GCACGAAAAA ATGTGAAACC ATCCGAGAGC TTTGCGAACA TCCTTGCGCA 
CTTCCATGTC ACGAAGATTC TCCGTGTGAG CCAAGCCCAT GCAAAGCAAG TGTGAGAATT 
TCTTGTGAAT GTGGACGAAT TAAAAAGGAC GCACCATGCT GTGAAGTTGA TAAAATGATT 
CTATCAAAAC TCGAAAAGGA AGAAGAAGAA AAATCTGAAT CGGATGGTGA AGAGAAAGTG 
GTCAAAGAAG GAATTCTCAA GCGCTCAACA AGTTTTTCTC AACTTAACTG CATGAAATGT 
GATGACGAAT GCAAGAAATT GGAAAGAAAT CGAAAAGTTG CTGAAGCTCT TGAAGTTGAT 
ACAGATGAAT ATGGAATGAA CAAACTCGCT CCAACTATTT CCTTCCCTTG TTATCTGAAA 
GAGATGGTTA GAACCAACAT TGATTTCGTC AAATCTGTTG AAAAGATTCT CATTGATCTG 
GTTATTCAAA TTCTAAGTGG TGAAGCCTAC CACGACACTT TTCGAGCTCA CCTGCCAGCA 
ATGAGTATTG AGAAGAGAAG ATTCGTGCAT GAATACGCCA ACTTTTTCAA TATCGCCTCA 
GAAAGTGTAG ACAGTCCACC AAAAAGAAGT ATCGTTCTCA CAGCAGTACG AGGAAAATCT 
CATCAGCCTC TCGTTCTCAT TAGTGATCTT GTAAACTACA AAGGAGCTCT AAAGACGCCA 
GGACCTGCTG TTATTAGAAA AGATATAATG GATCAAGCTT TGAGTAAAAA AGAAGAAACA 
GAAGGTCTCA TGAAACCATT AAGATGTACG GAAAAAATGG TAGTTCGGAG AGAAGCTCGT 
CCAATGAAGG AAATAACTGC CCCAATCCCA TTGAAACAAC AAAACCAATT TGCTCTTTTG 
GGGAGCGATG TCGATTCGGA CGATGAAGAA AGTAACGTTC CGACAACAAG TAATTTAGTA 
TCTTCACCTC CAAAAGATTG GTGGAAAGAC GAGGAAGAAG GGTGGCAAAA AGTGCAACAA 
AAAGAGTATG TTGTTGAAGT CGAACGCGAT ATGACCGAAG ATGAGATTGA GGCGGCGAAA 
AAACTGGATG AAGGTCCCAC ATGGGAAGAT CAATGTGACG AAGATGCACC AGCAACAGAA 
ACCAGTTCAG CTATTGAATC TCCAGCGAAA GAGGAGCCGG TTGCTGAATT ACTAGAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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nfx-1 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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