Nfx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nfx1
Entrez Gene ID	313166
Full Name	nuclear transcription factor, X-box binding 1
General protein information	Preferred Names nuclear transcription factor, X-box binding 1 Names transcriptional repressor NF-X1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024784.2	NP_001019955.2	transcriptional repressor NF-X1
XM_008763623.2	XP_008761845.1	transcriptional repressor NF-X1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFX1	XP_001159552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008728	XP_314840.4
Oryza sativa (rice)	Os06g0252300	NP_001057298.1
Bos taurus (cattle)	NFX1	NP_001095499.1
Rattus norvegicus (Norway rat)	Nfx1	NP_001019955.2
Drosophila melanogaster (fruit fly)	stc	NP_001260460.1
Kluyveromyces lactis	KLLA0E08317g	XP_454325.1
Xenopus tropicalis (tropical clawed frog)	nfx1	XP_002935327.2
Homo sapiens (human)	NFX1	NP_002495.2
Eremothecium gossypii	AGOS_ADL213W	NP_983883.2
Magnaporthe oryzae (rice blast fungus)	MGG_00137	XP_003718994.1
Arabidopsis thaliana (thale cress)	NFXL1	NP_172488.1
Gallus gallus (chicken)	NFX1	XP_004935125.1
Saccharomyces cerevisiae (baker's yeast)	FAP1	NP_014375.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.03	NP_588382.1
Canis lupus familiaris (dog)	NFX1	XP_005626763.1
Mus musculus (house mouse)	Nfx1	NP_076228.2
Danio rerio (zebrafish)	LOC562071	XP_690559.4
Caenorhabditis elegans (roundworm)	nfx-1	NP_498394.1

Gene Ontology
Bibliography

Related articles in PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression.
Arlotta P, Miyazaki D, Copeland NG, Gilbert DJ, Jenkins NA, Ono SJ
Immunology106(2)173-81(2002 Jun)

A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor.
Song Z, Krishna S, Thanos D, Strominger JL, Ono SJ
The Journal of experimental medicine180(5)1763-74(1994 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nfx1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfx1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa47190	XM_008763623.2 Latest version!	Rattus norvegicus nuclear transcription factor, X-box binding 1 (Nfx1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa06655	NM_001024784.2 Latest version!	Rattus norvegicus nuclear transcription factor, X-box binding 1 (Nfx1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nfx1 ( XM_008763623.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761845.1 Rattus norvegicus nuclear transcription factor, X-box binding 1 (Nfx1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47190
Clone ID Related Accession (Same CDS sequence)	XM_008763623.2
Accession Version	XM_008763623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional repressor NF-X1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCGGT CTCAGGCACT TTTAAATTTA ATACAGATGC TGCTGAGTTC 
ATTCCGCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGGA CCCAAAGAAG ACTAGATTCT 
AATAGGATTG GCAGAAGAAA TTATAGTTCC TCACCTCCCT GCCACCTTCC TAGACACATC 
CCTTACGATG ATGTCTCTGC TGTTCACCAG CACGGTTATG CCTCCGGAAG CAAGCCTAAG 
AGTCCACAGG GTTTTTTCCA GTCATCTAAT AAATCACTCA AGAACCATGG GCTTCAGAAT 
CAGCCATGGC AGAAATTAAG GAATGAAAAG CACCAAAACA GAATCAAGAA AGCACAAGGG 
CTCACTGAGC AGACGTCAGA TACATCCAGT TTAGAAAGCG TAGCCAGATC CGAGAGTGGA 
ACAAACCCTA GAGAACACAG CCCTTCTGAG AGCGAGAAGG AGGTAGTTAT TGCAGATCCC 
AGGGGAGCGA AACCCAAAAA AGCCACGCAG CTTACATACA ACTATGGCCG AGGGCCAAAG 
GCCAAAGGGA GACTCAGAAG CGAGTGGGGT AACAGAATGA GCCCAAAGTC TGAGGATGAA 
AATGCCAGAC CTGTGGCCAT TTCCCACTCT GACTCTTCAG AAGCCTCCTG TAGAAAACCT 
GTAGTGGATC CATGTGTGTG CAGACGGAGT GAGCAGAGAA GATATCCCCA GAAAAGGCCT 
CCCTGGGAAG TAGAGGGGGC CAGACCACGG CCAGGCAGGA ACCCACCGAA ACAGGAGAGT 
CAGCGGCATA TAAATGCAGG GCCCAAAAAC AACATGCCCC CCCTTCCAAA GGATAATCTC 
AGAGAGAGGC CAACGAAGTC AGCCTGTGAC ACTGGGAATT TGGCAGTTGT CACCAAGTCT 
TCCAGAAGAG TTGACCAAGA GAAAAATGCT GGAAGGAGAC AGGATCCTCA AGTGCTGTCT 
CCTTTTCCCA GAGGCAAGCA GAACCATATG CTGAAGAATG TGGAAACTCA TACAGGTTCC 
CTGATTGAGC AGCTAACTAC AGAAAAGTAC GAGTGTATGG TGTGCTGCGA GTTGGTTCAG 
GTCACAGCCC CGGTGTGGAG CTGCCAGAGC TGTTTCCATG TCTTTCATCT GAACTGCATC 
AAGAAATGGG CTCGCTCTCC AGCGTCCCAC GCAGATGGCC AGAGTGGTTG GAGGTGCCCC 
GCTTGTCAGA ATGTTTCTGC ACATGTTCCC AGTACCTACA CTTGTTTCTG TGGCAAAGTA 
AAGAACCCTG AATGGAGCAG AAATGAAATT CCGCACAGTT GTGGTGAAGT TTGTAGAAAG 
AAACAGCCTG GCCAGGACTG TCCACATTCC TGTAATCTTC TCTGCCATCC TGGACCCTGC 
CCACCCTGCC CTGCTTTTAC AACTAAAACA TGTGAATGTG GACGGACCAG GCACACGGTT 
CGCTGTGGTC AGCCTGTCTC AGTCCACTGT TCTAATGTGT GTGAGAATAT TTTGAACTGT 
GGTCAGCACC GCTGTGCTGA GCTGTGCCAT GCGGGTCAGT GCCAGCCTTG CCGGATCATA 
TTGAACCAGG TGTGCTACTG TGGCAGCACC TCCCGAGATG TGTTGTGTGG AACCGATGTG 
GGCAAGTCTG ATGGATTTGG GGACTTCAGC TGTTTAAAGA TATGTGGCAA GGACTTGAAA 
TGTGGGAGCC ATACCTGTTC TCAGGTTTGC CACCCTCAGC CCTGTCAGCC TTGCCCAAGG 
CTCCCCCATT TGGTGCGATA TTGCCCTTGC GGCCAAACAC CTCTCAGCCA GTTGCTGGAA 
CATGGAAGTA ATGGTCGGAA GACGTGCATG GATCCTGTTC CTTCCTGTGG AAAAGTGTGT 
GGCAAACCCT TGTCCTGTGG GTCCTCAGAT TTCATCCACA CCTGTGAAAA GCTCTGCCAT 
GAGGGAGACT GTGGGCCGTG CTCGCGCACA TCAGTCATTT CCTGCAGATG CTCCTTCAGA 
ACAAAGGAGC TCCCGTGTAC CAGTCTTAAA AGTGAAGATG CTACATTCAT GTGTGACAAG 
CGGTGTAACA AGAAACGATT GTGTGGACGG CATAAATGTA ACGAGATCTG CTGTGTGGAC 
AAGGAGCACA AGTGTCCTTT GATTTGTGGG AGGAAACTCC GTTGTGGCCT TCATCGGTGT 
GAAGAACCTT GTCACCGAGG GAACTGTCAG ACCTGCTGGC AAGCCAGCTT TGATGAATTA 
ACCTGCCATT GTGGCGCCTC TGTGATCTAC CCACCAGTTC CCTGTGGGAC TAGGCCTCCT 
GAGTGTACCC AGACCTGTGC GAGGATCCAT GAATGTGACC ATCCAGTATA TCATTCTTGT 
CATAGTGAGG AGAAGTGTCC CCCTTGTACT TTCCTAACTC AGAAGTGGTG CATGGGCAAG 
CATGAGGAGC TCACAATTAA GAAACTTTGG ACTTTTAAAG AGACTTTGGA CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761845.1
CDS	386..2842
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGTQRRLDSNRIGRRNYSSSPPCHLPRHIPYDDVSAVHQHGYASGSKPKSPQGFFQSSNKSLKNHGLQNQPWQKLRNEKHQNRIKKAQGLTEQTSDTSSLESVARSESGTNPREHSPSESEKEVVIADPRGAKPKKATQLTYNYGRGPKAKGRLRSEWGNRMSPKSEDENARPVAISHSDSSEASCRKPVVDPCVCRRSEQRRYPQKRPPWEVEGARPRPGRNPPKQESQRHINAGPKNNMPPLPKDNLRERPTKSACDTGNLAVVTKSSRRVDQEKNAGRRQDPQVLSPFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVQVTAPVWSCQSCFHVFHLNCIKKWARSPASHADGQSGWRCPACQNVSAHVPSTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFTTKTCECGRTRHTVRCGQPVSVHCSNVCENILNCGQHRCAELCHAGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGSHTCSQVCHPQPCQPCPRLPHLVRYCPCGQTPLSQLLEHGSNGRKTCMDPVPSCGKVCGKPLSCGSSDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARIHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHEELTIKKLWTFKETLDF

Target ORF information:

RefSeq Version	XM_008763623.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear transcription factor, X-box binding 1 (Nfx1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763623.2

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCGGT CTCAGGCACT TTTAAATTTA ATACAGATGC TGCTGAGTTC 
ATTCCGCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGGA CCCAAAGAAG ACTAGATTCT 
AATAGGATTG GCAGAAGAAA TTATAGTTCC TCACCTCCCT GCCACCTTCC TAGACACATC 
CCTTACGATG ATGTCTCTGC TGTTCACCAG CACGGTTATG CCTCCGGAAG CAAGCCTAAG 
AGTCCACAGG GTTTTTTCCA GTCATCTAAT AAATCACTCA AGAACCATGG GCTTCAGAAT 
CAGCCATGGC AGAAATTAAG GAATGAAAAG CACCAAAACA GAATCAAGAA AGCACAAGGG 
CTCACTGAGC AGACGTCAGA TACATCCAGT TTAGAAAGCG TAGCCAGATC CGAGAGTGGA 
ACAAACCCTA GAGAACACAG CCCTTCTGAG AGCGAGAAGG AGGTAGTTAT TGCAGATCCC 
AGGGGAGCGA AACCCAAAAA AGCCACGCAG CTTACATACA ACTATGGCCG AGGGCCAAAG 
GCCAAAGGGA GACTCAGAAG CGAGTGGGGT AACAGAATGA GCCCAAAGTC TGAGGATGAA 
AATGCCAGAC CTGTGGCCAT TTCCCACTCT GACTCTTCAG AAGCCTCCTG TAGAAAACCT 
GTAGTGGATC CATGTGTGTG CAGACGGAGT GAGCAGAGAA GATATCCCCA GAAAAGGCCT 
CCCTGGGAAG TAGAGGGGGC CAGACCACGG CCAGGCAGGA ACCCACCGAA ACAGGAGAGT 
CAGCGGCATA TAAATGCAGG GCCCAAAAAC AACATGCCCC CCCTTCCAAA GGATAATCTC 
AGAGAGAGGC CAACGAAGTC AGCCTGTGAC ACTGGGAATT TGGCAGTTGT CACCAAGTCT 
TCCAGAAGAG TTGACCAAGA GAAAAATGCT GGAAGGAGAC AGGATCCTCA AGTGCTGTCT 
CCTTTTCCCA GAGGCAAGCA GAACCATATG CTGAAGAATG TGGAAACTCA TACAGGTTCC 
CTGATTGAGC AGCTAACTAC AGAAAAGTAC GAGTGTATGG TGTGCTGCGA GTTGGTTCAG 
GTCACAGCCC CGGTGTGGAG CTGCCAGAGC TGTTTCCATG TCTTTCATCT GAACTGCATC 
AAGAAATGGG CTCGCTCTCC AGCGTCCCAC GCAGATGGCC AGAGTGGTTG GAGGTGCCCC 
GCTTGTCAGA ATGTTTCTGC ACATGTTCCC AGTACCTACA CTTGTTTCTG TGGCAAAGTA 
AAGAACCCTG AATGGAGCAG AAATGAAATT CCGCACAGTT GTGGTGAAGT TTGTAGAAAG 
AAACAGCCTG GCCAGGACTG TCCACATTCC TGTAATCTTC TCTGCCATCC TGGACCCTGC 
CCACCCTGCC CTGCTTTTAC AACTAAAACA TGTGAATGTG GACGGACCAG GCACACGGTT 
CGCTGTGGTC AGCCTGTCTC AGTCCACTGT TCTAATGTGT GTGAGAATAT TTTGAACTGT 
GGTCAGCACC GCTGTGCTGA GCTGTGCCAT GCGGGTCAGT GCCAGCCTTG CCGGATCATA 
TTGAACCAGG TGTGCTACTG TGGCAGCACC TCCCGAGATG TGTTGTGTGG AACCGATGTG 
GGCAAGTCTG ATGGATTTGG GGACTTCAGC TGTTTAAAGA TATGTGGCAA GGACTTGAAA 
TGTGGGAGCC ATACCTGTTC TCAGGTTTGC CACCCTCAGC CCTGTCAGCC TTGCCCAAGG 
CTCCCCCATT TGGTGCGATA TTGCCCTTGC GGCCAAACAC CTCTCAGCCA GTTGCTGGAA 
CATGGAAGTA ATGGTCGGAA GACGTGCATG GATCCTGTTC CTTCCTGTGG AAAAGTGTGT 
GGCAAACCCT TGTCCTGTGG GTCCTCAGAT TTCATCCACA CCTGTGAAAA GCTCTGCCAT 
GAGGGAGACT GTGGGCCGTG CTCGCGCACA TCAGTCATTT CCTGCAGATG CTCCTTCAGA 
ACAAAGGAGC TCCCGTGTAC CAGTCTTAAA AGTGAAGATG CTACATTCAT GTGTGACAAG 
CGGTGTAACA AGAAACGATT GTGTGGACGG CATAAATGTA ACGAGATCTG CTGTGTGGAC 
AAGGAGCACA AGTGTCCTTT GATTTGTGGG AGGAAACTCC GTTGTGGCCT TCATCGGTGT 
GAAGAACCTT GTCACCGAGG GAACTGTCAG ACCTGCTGGC AAGCCAGCTT TGATGAATTA 
ACCTGCCATT GTGGCGCCTC TGTGATCTAC CCACCAGTTC CCTGTGGGAC TAGGCCTCCT 
GAGTGTACCC AGACCTGTGC GAGGATCCAT GAATGTGACC ATCCAGTATA TCATTCTTGT 
CATAGTGAGG AGAAGTGTCC CCCTTGTACT TTCCTAACTC AGAAGTGGTG CATGGGCAAG 
CATGAGGAGC TCACAATTAA GAAACTTTGG ACTTTTAAAG AGACTTTGGA CTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nfx1 ( NM_001024784.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019955.2 Rattus norvegicus nuclear transcription factor, X-box binding 1 (Nfx1), mRNA.

Price & Availability↑

CloneID	ORa06655
Clone ID Related Accession (Same CDS sequence)	NM_001024784.2
Accession Version	NM_001024784.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	transcriptional repressor NF-X1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC121205.5, AABR07073103.1, AABR07048018.1 and AABR07048019.1. On Sep 11, 2008 this sequence version replaced NM_001024784.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG CGCCTCCGGT CTCAGGCACT TTTAAATTTA ATACAGATGC TGCTGAGTTC 
ATTCCGCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGGA CCCAAAGAAG ACTAGATTCT 
AATAGGATTG GCAGAAGAAA TTATAGTTCC TCACCTCCCT GCCACCTTCC TAGACACATC 
CCTTACGATG ATGTCTCTGC TGTTCACCAG CACGGTTATG CCTCCGGAAG CAAGCCTAAG 
AGTCCACAGG GTTTTTTCCA GTCATCTAAT AAATCACTCA AGAACCATGG GCTTCAGAAT 
CAGCCATGGC AGAAATTAAG GAATGAAAAG CACCAAAACA GAATCAAGAA AGCACAAGGG 
CTCACTGAGC AGACGTCAGA TACATCCAGT TTAGAAAGCG TAGCCAGATC CGAGAGTGGA 
ACAAACCCTA GAGAACACAG CCCTTCTGAG AGCGAGAAGG AGGTAGTTAT TGCAGATCCC 
AGGGGAGCGA AACCCAAAAA AGCCACGCAG CTTACATACA ACTATGGCCG AGGGCCAAAG 
GCCAAAGGGA GACTCAGAAG CGAGTGGGGT AACAGAATGA GCCCAAAGTC TGAGGATGAA 
AATGCCAGAC CTGTGGCCAT TTCCCACTCT GACTCTTCAG AAGCCTCCTG TAGAAAACCT 
GTAGTGGATC CATGTGTGTG CAGACGGAGT GAGCAGAGAA GATATCCCCA GAAAAGGCCT 
CCCTGGGAAG TAGAGGGGGC CAGACCACGG CCAGGCAGGA ACCCACCGAA ACAGGAGAGT 
CAGCGGCATA TAAATGCAGG GCCCAAAAAC AACATGCCCC CCCTTCCAAA GGATAATCTC 
AGAGAGAGGC CAACGAAGTC AGCCTGTGAC ACTGGGAATT TGGCAGTTGT CACCAAGTCT 
TCCAGAAGAG TTGACCAAGA GAAAAATGCT GGAAGGAGAC AGGATCCTCA AGTGCTGTCT 
CCTTTTCCCA GAGGCAAGCA GAACCATATG CTGAAGAATG TGGAAACTCA TACAGGTTCC 
CTGATTGAGC AGCTAACTAC AGAAAAGTAC GAGTGTATGG TGTGCTGCGA GTTGGTTCAG 
GTCACAGCCC CGGTGTGGAG CTGCCAGAGC TGTTTCCATG TCTTTCATCT GAACTGCATC 
AAGAAATGGG CTCGCTCTCC AGCGTCCCAC GCAGATGGCC AGAGTGGTTG GAGGTGCCCC 
GCTTGTCAGA ATGTTTCTGC ACATGTTCCC AGTACCTACA CTTGTTTCTG TGGCAAAGTA 
AAGAACCCTG AATGGAGCAG AAATGAAATT CCGCACAGTT GTGGTGAAGT TTGTAGAAAG 
AAACAGCCTG GCCAGGACTG TCCACATTCC TGTAATCTTC TCTGCCATCC TGGACCCTGC 
CCACCCTGCC CTGCTTTTAC AACTAAAACA TGTGAATGTG GACGGACCAG GCACACGGTT 
CGCTGTGGTC AGCCTGTCTC AGTCCACTGT TCTAATGTGT GTGAGAATAT TTTGAACTGT 
GGTCAGCACC GCTGTGCTGA GCTGTGCCAT GCGGGTCAGT GCCAGCCTTG CCGGATCATA 
TTGAACCAGG TGTGCTACTG TGGCAGCACC TCCCGAGATG TGTTGTGTGG AACCGATGTG 
GGCAAGTCTG ATGGATTTGG GGACTTCAGC TGTTTAAAGA TATGTGGCAA GGACTTGAAA 
TGTGGGAGCC ATACCTGTTC TCAGGTTTGC CACCCTCAGC CCTGTCAGCC TTGCCCAAGG 
CTCCCCCATT TGGTGCGATA TTGCCCTTGC GGCCAAACAC CTCTCAGCCA GTTGCTGGAA 
CATGGAAGTA ATGGTCGGAA GACGTGCATG GATCCTGTTC CTTCCTGTGG AAAAGTGTGT 
GGCAAACCCT TGTCCTGTGG GTCCTCAGAT TTCATCCACA CCTGTGAAAA GCTCTGCCAT 
GAGGGAGACT GTGGGCCGTG CTCGCGCACA TCAGTCATTT CCTGCAGATG CTCCTTCAGA 
ACAAAGGAGC TCCCGTGTAC CAGTCTTAAA AGTGAAGATG CTACATTCAT GTGTGACAAG 
CGGTGTAACA AGAAACGATT GTGTGGACGG CATAAATGTA ACGAGATCTG CTGTGTGGAC 
AAGGAGCACA AGTGTCCTTT GATTTGTGGG AGGAAACTCC GTTGTGGCCT TCATCGGTGT 
GAAGAACCTT GTCACCGAGG GAACTGTCAG ACCTGCTGGC AAGCCAGCTT TGATGAATTA 
ACCTGCCATT GTGGCGCCTC TGTGATCTAC CCACCAGTTC CCTGTGGGAC TAGGCCTCCT 
GAGTGTACCC AGACCTGTGC GAGGATCCAT GAATGTGACC ATCCAGTATA TCATTCTTGT 
CATAGTGAGG AGAAGTGTCC CCCTTGTACT TTCCTAACTC AGAAGTGGTG CATGGGCAAG 
CATGAGTTAC GAAGCAACAT CCCCTGTCAC CTGGTTGATA TCTCTTGCGG ATTACCCTGC 
AGTGCCATGC TACCCTGTGG GATGCACAAA TGTCAAAGAC TCTGTCACAA AGGAGAATGT 
CTTGTGGATG AGGCCTGCAA GCAGCCCTGT ACCACACCCA GAGCTGACTG TGGCCACCCA 
TGTATGGCTC CCTGCCACCC CAGTTTACCC TGCCCTGTGA CAGCTTGTAA AGCCAAGGTA 
GAGTTACAGT GTGAATGTGG AAGAAGAAAG GAGATGGTGA TTTGCTCTGA AGCATCCGGG 
ACTTATCAAA GGATAGCTGC CATTTCGATG GCCTCTAAAA TAACGGACAT GCAGCTTGGA 
GACTCAGTGG AGATCAGCAA GTTCATTACC AAGAAGGAAG TCCAACAAGC CAGGTTGCAG 
TGTGATGAGG AGTGTGCTGC CCTGGAAAGG AAAAAGAGAT TGGCAGAGGC ATTCGACATC 
ACTGATGACT CTGATCCTTT CAACGTACGC TCTTCAGCAT CGAAATTCAG TGACAGCCTG 
AAAGACGATG CCAGGAAAGA CTTCAAGTTT GTCAGTGACA TCGAGAAGGA AATGGAAGCG 
CTTGTGGAGG CCGTGAATAA GGGAAAGAGT AATAAGAAAA GCTACTGCTT CCCTCCCATG 
AACAGAGACC ACCGCCGCAT CATCCATGAC CTAGCCCAGG TTTATGGCCT GGAGAGTGTG 
AGCTATGACA GTGAACCAAA GCGCAATGTC GTGGTCACTG CCATCAGAGG GAAGTCCATC 
TGTCCTCCTA CCACGCTGAC CAGTGTTATT GAAAGGGAGA CACAGACACG GCCTCCACCA 
CCAATTCCTC ATCACCGACA CCAAGCAGAC AAGAATCCTG GGAGCAGTAA TTTACAGAAG 
ATCGTGAAGG AGCCTGTGAT TGACTACTTT GACGTCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019955.2
CDS	146..3490
Translation	MAEAPPVSGTFKFNTDAAEFIPQERKNSGLNCGTQRRLDSNRIGRRNYSSSPPCHLPRHIPYDDVSAVHQHGYASGSKPKSPQGFFQSSNKSLKNHGLQNQPWQKLRNEKHQNRIKKAQGLTEQTSDTSSLESVARSESGTNPREHSPSESEKEVVIADPRGAKPKKATQLTYNYGRGPKAKGRLRSEWGNRMSPKSEDENARPVAISHSDSSEASCRKPVVDPCVCRRSEQRRYPQKRPPWEVEGARPRPGRNPPKQESQRHINAGPKNNMPPLPKDNLRERPTKSACDTGNLAVVTKSSRRVDQEKNAGRRQDPQVLSPFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVQVTAPVWSCQSCFHVFHLNCIKKWARSPASHADGQSGWRCPACQNVSAHVPSTYTCFCGKVKNPEWSRNEIPHSCGEVCRKKQPGQDCPHSCNLLCHPGPCPPCPAFTTKTCECGRTRHTVRCGQPVSVHCSNVCENILNCGQHRCAELCHAGQCQPCRIILNQVCYCGSTSRDVLCGTDVGKSDGFGDFSCLKICGKDLKCGSHTCSQVCHPQPCQPCPRLPHLVRYCPCGQTPLSQLLEHGSNGRKTCMDPVPSCGKVCGKPLSCGSSDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARIHECDHPVYHSCHSEEKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCSAMLPCGMHKCQRLCHKGECLVDEACKQPCTTPRADCGHPCMAPCHPSLPCPVTACKAKVELQCECGRRKEMVICSEASGTYQRIAAISMASKITDMQLGDSVEISKFITKKEVQQARLQCDEECAALERKKRLAEAFDITDDSDPFNVRSSASKFSDSLKDDARKDFKFVSDIEKEMEALVEAVNKGKSNKKSYCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSICPPTTLTSVIERETQTRPPPPIPHHRHQADKNPGSSNLQKIVKEPVIDYFDVQD

Target ORF information:

RefSeq Version	NM_001024784.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear transcription factor, X-box binding 1 (Nfx1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024784.2

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCGGT CTCAGGCACT TTTAAATTTA ATACAGATGC TGCTGAGTTC 
ATTCCGCAGG AGAGAAAAAA TTCTGGTCTA AATTGTGGGA CCCAAAGAAG ACTAGATTCT 
AATAGGATTG GCAGAAGAAA TTATAGTTCC TCACCTCCCT GCCACCTTCC TAGACACATC 
CCTTACGATG ATGTCTCTGC TGTTCACCAG CACGGTTATG CCTCCGGAAG CAAGCCTAAG 
AGTCCACAGG GTTTTTTCCA GTCATCTAAT AAATCACTCA AGAACCATGG GCTTCAGAAT 
CAGCCATGGC AGAAATTAAG GAATGAAAAG CACCAAAACA GAATCAAGAA AGCACAAGGG 
CTCACTGAGC AGACGTCAGA TACATCCAGT TTAGAAAGCG TAGCCAGATC CGAGAGTGGA 
ACAAACCCTA GAGAACACAG CCCTTCTGAG AGCGAGAAGG AGGTAGTTAT TGCAGATCCC 
AGGGGAGCGA AACCCAAAAA AGCCACGCAG CTTACATACA ACTATGGCCG AGGGCCAAAG 
GCCAAAGGGA GACTCAGAAG CGAGTGGGGT AACAGAATGA GCCCAAAGTC TGAGGATGAA 
AATGCCAGAC CTGTGGCCAT TTCCCACTCT GACTCTTCAG AAGCCTCCTG TAGAAAACCT 
GTAGTGGATC CATGTGTGTG CAGACGGAGT GAGCAGAGAA GATATCCCCA GAAAAGGCCT 
CCCTGGGAAG TAGAGGGGGC CAGACCACGG CCAGGCAGGA ACCCACCGAA ACAGGAGAGT 
CAGCGGCATA TAAATGCAGG GCCCAAAAAC AACATGCCCC CCCTTCCAAA GGATAATCTC 
AGAGAGAGGC CAACGAAGTC AGCCTGTGAC ACTGGGAATT TGGCAGTTGT CACCAAGTCT 
TCCAGAAGAG TTGACCAAGA GAAAAATGCT GGAAGGAGAC AGGATCCTCA AGTGCTGTCT 
CCTTTTCCCA GAGGCAAGCA GAACCATATG CTGAAGAATG TGGAAACTCA TACAGGTTCC 
CTGATTGAGC AGCTAACTAC AGAAAAGTAC GAGTGTATGG TGTGCTGCGA GTTGGTTCAG 
GTCACAGCCC CGGTGTGGAG CTGCCAGAGC TGTTTCCATG TCTTTCATCT GAACTGCATC 
AAGAAATGGG CTCGCTCTCC AGCGTCCCAC GCAGATGGCC AGAGTGGTTG GAGGTGCCCC 
GCTTGTCAGA ATGTTTCTGC ACATGTTCCC AGTACCTACA CTTGTTTCTG TGGCAAAGTA 
AAGAACCCTG AATGGAGCAG AAATGAAATT CCGCACAGTT GTGGTGAAGT TTGTAGAAAG 
AAACAGCCTG GCCAGGACTG TCCACATTCC TGTAATCTTC TCTGCCATCC TGGACCCTGC 
CCACCCTGCC CTGCTTTTAC AACTAAAACA TGTGAATGTG GACGGACCAG GCACACGGTT 
CGCTGTGGTC AGCCTGTCTC AGTCCACTGT TCTAATGTGT GTGAGAATAT TTTGAACTGT 
GGTCAGCACC GCTGTGCTGA GCTGTGCCAT GCGGGTCAGT GCCAGCCTTG CCGGATCATA 
TTGAACCAGG TGTGCTACTG TGGCAGCACC TCCCGAGATG TGTTGTGTGG AACCGATGTG 
GGCAAGTCTG ATGGATTTGG GGACTTCAGC TGTTTAAAGA TATGTGGCAA GGACTTGAAA 
TGTGGGAGCC ATACCTGTTC TCAGGTTTGC CACCCTCAGC CCTGTCAGCC TTGCCCAAGG 
CTCCCCCATT TGGTGCGATA TTGCCCTTGC GGCCAAACAC CTCTCAGCCA GTTGCTGGAA 
CATGGAAGTA ATGGTCGGAA GACGTGCATG GATCCTGTTC CTTCCTGTGG AAAAGTGTGT 
GGCAAACCCT TGTCCTGTGG GTCCTCAGAT TTCATCCACA CCTGTGAAAA GCTCTGCCAT 
GAGGGAGACT GTGGGCCGTG CTCGCGCACA TCAGTCATTT CCTGCAGATG CTCCTTCAGA 
ACAAAGGAGC TCCCGTGTAC CAGTCTTAAA AGTGAAGATG CTACATTCAT GTGTGACAAG 
CGGTGTAACA AGAAACGATT GTGTGGACGG CATAAATGTA ACGAGATCTG CTGTGTGGAC 
AAGGAGCACA AGTGTCCTTT GATTTGTGGG AGGAAACTCC GTTGTGGCCT TCATCGGTGT 
GAAGAACCTT GTCACCGAGG GAACTGTCAG ACCTGCTGGC AAGCCAGCTT TGATGAATTA 
ACCTGCCATT GTGGCGCCTC TGTGATCTAC CCACCAGTTC CCTGTGGGAC TAGGCCTCCT 
GAGTGTACCC AGACCTGTGC GAGGATCCAT GAATGTGACC ATCCAGTATA TCATTCTTGT 
CATAGTGAGG AGAAGTGTCC CCCTTGTACT TTCCTAACTC AGAAGTGGTG CATGGGCAAG 
CATGAGTTAC GAAGCAACAT CCCCTGTCAC CTGGTTGATA TCTCTTGCGG ATTACCCTGC 
AGTGCCATGC TACCCTGTGG GATGCACAAA TGTCAAAGAC TCTGTCACAA AGGAGAATGT 
CTTGTGGATG AGGCCTGCAA GCAGCCCTGT ACCACACCCA GAGCTGACTG TGGCCACCCA 
TGTATGGCTC CCTGCCACCC CAGTTTACCC TGCCCTGTGA CAGCTTGTAA AGCCAAGGTA 
GAGTTACAGT GTGAATGTGG AAGAAGAAAG GAGATGGTGA TTTGCTCTGA AGCATCCGGG 
ACTTATCAAA GGATAGCTGC CATTTCGATG GCCTCTAAAA TAACGGACAT GCAGCTTGGA 
GACTCAGTGG AGATCAGCAA GTTCATTACC AAGAAGGAAG TCCAACAAGC CAGGTTGCAG 
TGTGATGAGG AGTGTGCTGC CCTGGAAAGG AAAAAGAGAT TGGCAGAGGC ATTCGACATC 
ACTGATGACT CTGATCCTTT CAACGTACGC TCTTCAGCAT CGAAATTCAG TGACAGCCTG 
AAAGACGATG CCAGGAAAGA CTTCAAGTTT GTCAGTGACA TCGAGAAGGA AATGGAAGCG 
CTTGTGGAGG CCGTGAATAA GGGAAAGAGT AATAAGAAAA GCTACTGCTT CCCTCCCATG 
AACAGAGACC ACCGCCGCAT CATCCATGAC CTAGCCCAGG TTTATGGCCT GGAGAGTGTG 
AGCTATGACA GTGAACCAAA GCGCAATGTC GTGGTCACTG CCATCAGAGG GAAGTCCATC 
TGTCCTCCTA CCACGCTGAC CAGTGTTATT GAAAGGGAGA CACAGACACG GCCTCCACCA 
CCAATTCCTC ATCACCGACA CCAAGCAGAC AAGAATCCTG GGAGCAGTAA TTTACAGAAG 
ATCGTGAAGG AGCCTGTGAT TGACTACTTT GACGTCCAGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression.
Immunology106(2)173-81(2002 Jun)
Arlotta P,Miyazaki D,Copeland NG,Gilbert DJ,Jenkins NA,Ono SJ

A novel cysteine-rich sequence-specific DNA-binding protein interacts with the conserved X-box motif of the human major histocompatibility complex class II genes via a repeated Cys-His domain and functions as a transcriptional repressor.
The Journal of experimental medicine180(5)1763-74(1994 Nov)
Song Z,Krishna S,Thanos D,Strominger JL,Ono SJ

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Nfx1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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