NFX1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFX1
Entrez Gene ID	515680
Full Name	nuclear transcription factor, X-box binding 1
General protein information	Preferred Names nuclear transcription factor, X-box binding 1 Names transcriptional repressor NF-X1 nuclear transcription factor, X box-binding protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005210325.4	XP_005210382.1	transcriptional repressor NF-X1 isoform X2
XM_005210326.3	XP_005210383.1	transcriptional repressor NF-X1 isoform X3
XM_005210324.3	XP_005210381.1	transcriptional repressor NF-X1 isoform X1
XM_005210325.3	XP_005210382.1	transcriptional repressor NF-X1 isoform X2
NM_001102029.2	NP_001095499.1	transcriptional repressor NF-X1
XM_005210326.4	XP_005210383.1	transcriptional repressor NF-X1 isoform X3
XM_005210324.4	XP_005210381.1	transcriptional repressor NF-X1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NFX1	XP_001159552.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008728	XP_314840.4
Oryza sativa (rice)	Os06g0252300	NP_001057298.1
Bos taurus (cattle)	NFX1	NP_001095499.1
Rattus norvegicus (Norway rat)	Nfx1	NP_001019955.2
Drosophila melanogaster (fruit fly)	stc	NP_001260460.1
Kluyveromyces lactis	KLLA0E08317g	XP_454325.1
Xenopus tropicalis (tropical clawed frog)	nfx1	XP_002935327.2
Homo sapiens (human)	NFX1	NP_002495.2
Eremothecium gossypii	AGOS_ADL213W	NP_983883.2
Magnaporthe oryzae (rice blast fungus)	MGG_00137	XP_003718994.1
Arabidopsis thaliana (thale cress)	NFXL1	NP_172488.1
Gallus gallus (chicken)	NFX1	XP_004935125.1
Saccharomyces cerevisiae (baker's yeast)	FAP1	NP_014375.1
Schizosaccharomyces pombe (fission yeast)	SPCC18.03	NP_588382.1
Canis lupus familiaris (dog)	NFX1	XP_005626763.1
Mus musculus (house mouse)	Nfx1	NP_076228.2
Danio rerio (zebrafish)	LOC562071	XP_690559.4
Caenorhabditis elegans (roundworm)	nfx-1	NP_498394.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NFX1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFX1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa237075	XM_005210324.4 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa237076	XM_005210325.4 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa237077	XM_005210326.4 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa44715	NM_001102029.2 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa22218	XM_005210324.3 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa22219	XM_005210325.3 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa22220	XM_005210326.3 Latest version!	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFX1 ( XM_005210324.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210381.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa237075
Clone ID Related Accession (Same CDS sequence)	XM_005210324.4
Accession Version	XM_005210324.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210324.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGCA GATGCTACAT TTATGTGCGA CAAGAGGTGT 
AACAAGAAGC GGTTATGTGG ACGGCATAAA TGTAATGAGA TATGCTGTGT GGATAAGGAG 
CACAAGTGTC CTTTGATTTG TGGGAGGAAA CTCCGCTGTG GCCTTCATAG ATGTGAAGAA 
CCTTGTCATC GTGGAAACTG CCAGACGTGC TGGCAAGCTA GTTTTGATGA ATTAACCTGC 
CACTGTGGTG CATCAGTGAT CTACCCTCCA GTTCCCTGTG GCACCAGGCC CCCTGAGTGT 
ACCCAGACCT GTGCCAGAGT CCATGAATGT GACCATCCAG TGTATCATTC ATGTCATAGT 
GAGGACAAGT GCCCTCCTTG TACTTTCCTC ACCCAGAAGT GGTGCATGGG CAAACACGAG 
TTACGAAGCA ACATTCCCTG TCACCTGGTT GATATCTCTT GTGGATTACC CTGTGGTGCC 
ACGCTACCAT GTGGGATGCA CAAGTGTCAG AGGCTCTGTC ACAAAGGGGA ATGTCTGGGG 
GACGAGATCT GCAAGCAGCC CTGCACCACC TCCAGAGCCA ACTGTGGCCA CCCGTGTATG 
GCACCCTGCC ACCTCAGCTT GCCCTGCCCA GTGACCACCT GCAAGGCCAA GATAGAGCTG 
CAGTGTGAAT GTGGACGAAG AAAAGAAATC ATGGTTTGCT CAGAAGCATC CAGTACTTAT 
CAAAGAATAG CTGCTATTTC CATGGCCTCC AAAATAACAG ACATGCAGCT TGGAGATTCA 
GTGGAGATCA GCAAGTTGAT TACCAAGAAG GAAATTCACC AAGCCAGGCT GGAGTGTGAT 
GAGGAATGTT CAGCCTTGGA AAGGAAAAAG AGGTTAGCAG AGGCATTTCA TATCAGTGAT 
GATTCTGATC CTTTCAACGT ACGTTCTTCA GGGTCAAAAT TCAGTGACAG TTTGAAAGAA 
GATGCTAGGA AGGACTTAAG GTTTGTCAGC GACATTGAGA AGGAAATGGA AGCCCTTGTG 
GAGGCTGTGA ATAAGGGGAA AAGTAGTAAG AAGAGCCACT GCTTCCCTCC CATGAACAGA 
GACCACCGCC GGATCATCCA CGATCTGGCC CAGGTGTACG GCCTGGAGAG CGTGAGCTAT 
GACAGTGAGC CAAAGCGCAA CGTCGTGGTC ACTGCGGTCA GAGGGAAGTC CATTTGTCCT 
TCTACCACAC TGACAGGTGT ACTCGAAAAG GAGAATCAGT CACGGCCTCC GCCACCGATT 
GCTCATCACA GACAGACAGA CAAGAATCCT GGGAGCAGCA ATTTACAGAA GATAGCGAAG 
GAGCCCGTAA TTGACTACTT TGATGTCCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210381.1
CDS	137..3532
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEGMTGKGCDGTSWGEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005210324.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210324.4

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGCA GATGCTACAT TTATGTGCGA CAAGAGGTGT 
AACAAGAAGC GGTTATGTGG ACGGCATAAA TGTAATGAGA TATGCTGTGT GGATAAGGAG 
CACAAGTGTC CTTTGATTTG TGGGAGGAAA CTCCGCTGTG GCCTTCATAG ATGTGAAGAA 
CCTTGTCATC GTGGAAACTG CCAGACGTGC TGGCAAGCTA GTTTTGATGA ATTAACCTGC 
CACTGTGGTG CATCAGTGAT CTACCCTCCA GTTCCCTGTG GCACCAGGCC CCCTGAGTGT 
ACCCAGACCT GTGCCAGAGT CCATGAATGT GACCATCCAG TGTATCATTC ATGTCATAGT 
GAGGACAAGT GCCCTCCTTG TACTTTCCTC ACCCAGAAGT GGTGCATGGG CAAACACGAG 
TTACGAAGCA ACATTCCCTG TCACCTGGTT GATATCTCTT GTGGATTACC CTGTGGTGCC 
ACGCTACCAT GTGGGATGCA CAAGTGTCAG AGGCTCTGTC ACAAAGGGGA ATGTCTGGGG 
GACGAGATCT GCAAGCAGCC CTGCACCACC TCCAGAGCCA ACTGTGGCCA CCCGTGTATG 
GCACCCTGCC ACCTCAGCTT GCCCTGCCCA GTGACCACCT GCAAGGCCAA GATAGAGCTG 
CAGTGTGAAT GTGGACGAAG AAAAGAAATC ATGGTTTGCT CAGAAGCATC CAGTACTTAT 
CAAAGAATAG CTGCTATTTC CATGGCCTCC AAAATAACAG ACATGCAGCT TGGAGATTCA 
GTGGAGATCA GCAAGTTGAT TACCAAGAAG GAAATTCACC AAGCCAGGCT GGAGTGTGAT 
GAGGAATGTT CAGCCTTGGA AAGGAAAAAG AGGTTAGCAG AGGCATTTCA TATCAGTGAT 
GATTCTGATC CTTTCAACGT ACGTTCTTCA GGGTCAAAAT TCAGTGACAG TTTGAAAGAA 
GATGCTAGGA AGGACTTAAG GTTTGTCAGC GACATTGAGA AGGAAATGGA AGCCCTTGTG 
GAGGCTGTGA ATAAGGGGAA AAGTAGTAAG AAGAGCCACT GCTTCCCTCC CATGAACAGA 
GACCACCGCC GGATCATCCA CGATCTGGCC CAGGTGTACG GCCTGGAGAG CGTGAGCTAT 
GACAGTGAGC CAAAGCGCAA CGTCGTGGTC ACTGCGGTCA GAGGGAAGTC CATTTGTCCT 
TCTACCACAC TGACAGGTGT ACTCGAAAAG GAGAATCAGT CACGGCCTCC GCCACCGATT 
GCTCATCACA GACAGACAGA CAAGAATCCT GGGAGCAGCA ATTTACAGAA GATAGCGAAG 
GAGCCCGTAA TTGACTACTT TGATGTCCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005210325.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210382.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa237076
Clone ID Related Accession (Same CDS sequence)	XM_005210325.4
Accession Version	XM_005210325.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210325.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGAT GCTACATTTA TGTGCGACAA GAGGTGTAAC 
AAGAAGCGGT TATGTGGACG GCATAAATGT AATGAGATAT GCTGTGTGGA TAAGGAGCAC 
AAGTGTCCTT TGATTTGTGG GAGGAAACTC CGCTGTGGCC TTCATAGATG TGAAGAACCT 
TGTCATCGTG GAAACTGCCA GACGTGCTGG CAAGCTAGTT TTGATGAATT AACCTGCCAC 
TGTGGTGCAT CAGTGATCTA CCCTCCAGTT CCCTGTGGCA CCAGGCCCCC TGAGTGTACC 
CAGACCTGTG CCAGAGTCCA TGAATGTGAC CATCCAGTGT ATCATTCATG TCATAGTGAG 
GACAAGTGCC CTCCTTGTAC TTTCCTCACC CAGAAGTGGT GCATGGGCAA ACACGAGTTA 
CGAAGCAACA TTCCCTGTCA CCTGGTTGAT ATCTCTTGTG GATTACCCTG TGGTGCCACG 
CTACCATGTG GGATGCACAA GTGTCAGAGG CTCTGTCACA AAGGGGAATG TCTGGGGGAC 
GAGATCTGCA AGCAGCCCTG CACCACCTCC AGAGCCAACT GTGGCCACCC GTGTATGGCA 
CCCTGCCACC TCAGCTTGCC CTGCCCAGTG ACCACCTGCA AGGCCAAGAT AGAGCTGCAG 
TGTGAATGTG GACGAAGAAA AGAAATCATG GTTTGCTCAG AAGCATCCAG TACTTATCAA 
AGAATAGCTG CTATTTCCAT GGCCTCCAAA ATAACAGACA TGCAGCTTGG AGATTCAGTG 
GAGATCAGCA AGTTGATTAC CAAGAAGGAA ATTCACCAAG CCAGGCTGGA GTGTGATGAG 
GAATGTTCAG CCTTGGAAAG GAAAAAGAGG TTAGCAGAGG CATTTCATAT CAGTGATGAT 
TCTGATCCTT TCAACGTACG TTCTTCAGGG TCAAAATTCA GTGACAGTTT GAAAGAAGAT 
GCTAGGAAGG ACTTAAGGTT TGTCAGCGAC ATTGAGAAGG AAATGGAAGC CCTTGTGGAG 
GCTGTGAATA AGGGGAAAAG TAGTAAGAAG AGCCACTGCT TCCCTCCCAT GAACAGAGAC 
CACCGCCGGA TCATCCACGA TCTGGCCCAG GTGTACGGCC TGGAGAGCGT GAGCTATGAC 
AGTGAGCCAA AGCGCAACGT CGTGGTCACT GCGGTCAGAG GGAAGTCCAT TTGTCCTTCT 
ACCACACTGA CAGGTGTACT CGAAAAGGAG AATCAGTCAC GGCCTCCGCC ACCGATTGCT 
CATCACAGAC AGACAGACAA GAATCCTGGG AGCAGCAATT TACAGAAGAT AGCGAAGGAG 
CCCGTAATTG ACTACTTTGA TGTCCAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210382.1
CDS	137..3529
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEGMTGKGCDGTSWGEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005210325.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210325.4

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGAT GCTACATTTA TGTGCGACAA GAGGTGTAAC 
AAGAAGCGGT TATGTGGACG GCATAAATGT AATGAGATAT GCTGTGTGGA TAAGGAGCAC 
AAGTGTCCTT TGATTTGTGG GAGGAAACTC CGCTGTGGCC TTCATAGATG TGAAGAACCT 
TGTCATCGTG GAAACTGCCA GACGTGCTGG CAAGCTAGTT TTGATGAATT AACCTGCCAC 
TGTGGTGCAT CAGTGATCTA CCCTCCAGTT CCCTGTGGCA CCAGGCCCCC TGAGTGTACC 
CAGACCTGTG CCAGAGTCCA TGAATGTGAC CATCCAGTGT ATCATTCATG TCATAGTGAG 
GACAAGTGCC CTCCTTGTAC TTTCCTCACC CAGAAGTGGT GCATGGGCAA ACACGAGTTA 
CGAAGCAACA TTCCCTGTCA CCTGGTTGAT ATCTCTTGTG GATTACCCTG TGGTGCCACG 
CTACCATGTG GGATGCACAA GTGTCAGAGG CTCTGTCACA AAGGGGAATG TCTGGGGGAC 
GAGATCTGCA AGCAGCCCTG CACCACCTCC AGAGCCAACT GTGGCCACCC GTGTATGGCA 
CCCTGCCACC TCAGCTTGCC CTGCCCAGTG ACCACCTGCA AGGCCAAGAT AGAGCTGCAG 
TGTGAATGTG GACGAAGAAA AGAAATCATG GTTTGCTCAG AAGCATCCAG TACTTATCAA 
AGAATAGCTG CTATTTCCAT GGCCTCCAAA ATAACAGACA TGCAGCTTGG AGATTCAGTG 
GAGATCAGCA AGTTGATTAC CAAGAAGGAA ATTCACCAAG CCAGGCTGGA GTGTGATGAG 
GAATGTTCAG CCTTGGAAAG GAAAAAGAGG TTAGCAGAGG CATTTCATAT CAGTGATGAT 
TCTGATCCTT TCAACGTACG TTCTTCAGGG TCAAAATTCA GTGACAGTTT GAAAGAAGAT 
GCTAGGAAGG ACTTAAGGTT TGTCAGCGAC ATTGAGAAGG AAATGGAAGC CCTTGTGGAG 
GCTGTGAATA AGGGGAAAAG TAGTAAGAAG AGCCACTGCT TCCCTCCCAT GAACAGAGAC 
CACCGCCGGA TCATCCACGA TCTGGCCCAG GTGTACGGCC TGGAGAGCGT GAGCTATGAC 
AGTGAGCCAA AGCGCAACGT CGTGGTCACT GCGGTCAGAG GGAAGTCCAT TTGTCCTTCT 
ACCACACTGA CAGGTGTACT CGAAAAGGAG AATCAGTCAC GGCCTCCGCC ACCGATTGCT 
CATCACAGAC AGACAGACAA GAATCCTGGG AGCAGCAATT TACAGAAGAT AGCGAAGGAG 
CCCGTAATTG ACTACTTTGA TGTCCAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005210326.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210383.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.

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CloneID	OBa237077
Clone ID Related Accession (Same CDS sequence)	XM_005210326.4
Accession Version	XM_005210326.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210326.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGCGACA AGAGGTGTAA CAAGAAGCGG TTATGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGTCCT TTGATTTGTG GGAGGAAACT CCGCTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGAAACTGCC AGACGTGCTG GCAAGCTAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGC 
ACCAGGCCCC CTGAGTGTAC CCAGACCTGT GCCAGAGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCAT GTCATAGTGA GGACAAGTGC CCTCCTTGTA CTTTCCTCAC CCAGAAGTGG 
TGCATGGGCA AACACGAGTT ACGAAGCAAC ATTCCCTGTC ACCTGGTTGA TATCTCTTGT 
GGATTACCCT GTGGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG GCTCTGTCAC 
AAAGGGGAAT GTCTGGGGGA CGAGATCTGC AAGCAGCCCT GCACCACCTC CAGAGCCAAC 
TGTGGCCACC CGTGTATGGC ACCCTGCCAC CTCAGCTTGC CCTGCCCAGT GACCACCTGC 
AAGGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AAGAAATCAT GGTTTGCTCA 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCCAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAGATCAGC AAGTTGATTA CCAAGAAGGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAATGTTCA GCCTTGGAAA GGAAAAAGAG GTTAGCAGAG 
GCATTTCATA TCAGTGATGA TTCTGATCCT TTCAACGTAC GTTCTTCAGG GTCAAAATTC 
AGTGACAGTT TGAAAGAAGA TGCTAGGAAG GACTTAAGGT TTGTCAGCGA CATTGAGAAG 
GAAATGGAAG CCCTTGTGGA GGCTGTGAAT AAGGGGAAAA GTAGTAAGAA GAGCCACTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ATCTGGCCCA GGTGTACGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAGCCA AAGCGCAACG TCGTGGTCAC TGCGGTCAGA 
GGGAAGTCCA TTTGTCCTTC TACCACACTG ACAGGTGTAC TCGAAAAGGA GAATCAGTCA 
CGGCCTCCGC CACCGATTGC TCATCACAGA CAGACAGACA AGAATCCTGG GAGCAGCAAT 
TTACAGAAGA TAGCGAAGGA GCCCGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210383.1
CDS	137..3490
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005210326.4
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210326.4

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGCGACA AGAGGTGTAA CAAGAAGCGG TTATGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGTCCT TTGATTTGTG GGAGGAAACT CCGCTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGAAACTGCC AGACGTGCTG GCAAGCTAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGC 
ACCAGGCCCC CTGAGTGTAC CCAGACCTGT GCCAGAGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCAT GTCATAGTGA GGACAAGTGC CCTCCTTGTA CTTTCCTCAC CCAGAAGTGG 
TGCATGGGCA AACACGAGTT ACGAAGCAAC ATTCCCTGTC ACCTGGTTGA TATCTCTTGT 
GGATTACCCT GTGGTGCCAC GCTACCATGT GGGATGCACA AGTGTCAGAG GCTCTGTCAC 
AAAGGGGAAT GTCTGGGGGA CGAGATCTGC AAGCAGCCCT GCACCACCTC CAGAGCCAAC 
TGTGGCCACC CGTGTATGGC ACCCTGCCAC CTCAGCTTGC CCTGCCCAGT GACCACCTGC 
AAGGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AAGAAATCAT GGTTTGCTCA 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCCAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAGATCAGC AAGTTGATTA CCAAGAAGGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAATGTTCA GCCTTGGAAA GGAAAAAGAG GTTAGCAGAG 
GCATTTCATA TCAGTGATGA TTCTGATCCT TTCAACGTAC GTTCTTCAGG GTCAAAATTC 
AGTGACAGTT TGAAAGAAGA TGCTAGGAAG GACTTAAGGT TTGTCAGCGA CATTGAGAAG 
GAAATGGAAG CCCTTGTGGA GGCTGTGAAT AAGGGGAAAA GTAGTAAGAA GAGCCACTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ATCTGGCCCA GGTGTACGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAGCCA AAGCGCAACG TCGTGGTCAC TGCGGTCAGA 
GGGAAGTCCA TTTGTCCTTC TACCACACTG ACAGGTGTAC TCGAAAAGGA GAATCAGTCA 
CGGCCTCCGC CACCGATTGC TCATCACAGA CAGACAGACA AGAATCCTGG GAGCAGCAAT 
TTACAGAAGA TAGCGAAGGA GCCCGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( NM_001102029.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095499.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), mRNA.

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CloneID	OBa44715
Clone ID Related Accession (Same CDS sequence)	NM_001102029.2
Accession Version	NM_001102029.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC147890.1. On Aug 30, 2012 this sequence version replaced NM_001102029.1. ##Evidence-Data-START## Transcript exon combination :: BC147890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGATGC TACATTTATG 
TGCGACAAGA GGTGTAACAA GAAGCGGTTA TGTGGACGGC ATAAATGTAA TGAGATATGC 
TGTGTGGATA AGGAGCACAA GTGTCCTTTG ATTTGTGGGA GGAAACTCCG CTGTGGCCTT 
CATAGATGTG AAGAACCTTG TCATCGTGGA AACTGCCAGA CGTGCTGGCA AGCTAGTTTT 
GATGAATTAA CCTGCCACTG TGGTGCATCA GTGATCTACC CTCCAGTTCC CTGTGGCACC 
AGGCCCCCTG AGTGTACCCA GACCTGTGCC AGAGTCCATG AATGTGACCA TCCAGTGTAT 
CATTCATGTC ATAGTGAGGA CAAGTGCCCT CCTTGTACTT TCCTCACCCA GAAGTGGTGC 
ATGGGCAAAC ACGAGTTACG AAGCAACATT CCCTGTCACC TGGTTGATAT CTCTTGTGGA 
TTACCCTGTG GTGCCACGCT ACCATGTGGG ATGCACAAGT GTCAGAGGCT CTGTCACAAA 
GGGGAATGTC TGGGGGACGA GATCTGCAAG CAGCCCTGCA CCACCTCCAG AGCCAACTGT 
GGCCACCCGT GTATGGCACC CTGCCACCTC AGCTTGCCCT GCCCAGTGAC CACCTGCAAG 
GCCAAGATAG AGCTGCAGTG TGAATGTGGA CGAAGAAAAG AAATCATGGT TTGCTCAGAA 
GCATCCAGTA CTTATCAAAG AATAGCTGCT ATTTCCATGG CCTCCAAAAT AACAGACATG 
CAGCTTGGAG ATTCAGTGGA GATCAGCAAG TTGATTACCA AGAAGGAAAT TCACCAAGCC 
AGGCTGGAGT GTGATGAGGA ATGTTCAGCC TTGGAAAGGA AAAAGAGGTT AGCAGAGGCA 
TTTCATATCA GTGATGATTC TGATCCTTTC AACGTACGTT CTTCAGGGTC AAAATTCAGT 
GACAGTTTGA AAGAAGATGC TAGGAAGGAC TTAAGGTTTG TCAGCGACAT TGAGAAGGAA 
ATGGAAGCCC TTGTGGAGGC TGTGAATAAG GGGAAAAGTA GTAAGAAGAG CCACTGCTTC 
CCTCCCATGA ACAGAGACCA CCGCCGGATC ATCCACGATC TGGCCCAGGT GTACGGCCTG 
GAGAGCGTGA GCTATGACAG TGAGCCAAAG CGCAACGTCG TGGTCACTGC GGTCAGAGGG 
AAGTCCATTT GTCCTTCTAC CACACTGACA GGTGTACTCG AAAAGGAGAA TCAGTCACGG 
CCTCCGCCAC CGATTGCTCA TCACAGACAG ACAGACAAGA ATCCTGGGAG CAGCAATTTA 
CAGAAGATAG CGAAGGAGCC CGTAATTGAC TACTTTGATG TCCAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095499.1
CDS	62..3412
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	NM_001102029.2
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102029.2

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGATGC TACATTTATG 
TGCGACAAGA GGTGTAACAA GAAGCGGTTA TGTGGACGGC ATAAATGTAA TGAGATATGC 
TGTGTGGATA AGGAGCACAA GTGTCCTTTG ATTTGTGGGA GGAAACTCCG CTGTGGCCTT 
CATAGATGTG AAGAACCTTG TCATCGTGGA AACTGCCAGA CGTGCTGGCA AGCTAGTTTT 
GATGAATTAA CCTGCCACTG TGGTGCATCA GTGATCTACC CTCCAGTTCC CTGTGGCACC 
AGGCCCCCTG AGTGTACCCA GACCTGTGCC AGAGTCCATG AATGTGACCA TCCAGTGTAT 
CATTCATGTC ATAGTGAGGA CAAGTGCCCT CCTTGTACTT TCCTCACCCA GAAGTGGTGC 
ATGGGCAAAC ACGAGTTACG AAGCAACATT CCCTGTCACC TGGTTGATAT CTCTTGTGGA 
TTACCCTGTG GTGCCACGCT ACCATGTGGG ATGCACAAGT GTCAGAGGCT CTGTCACAAA 
GGGGAATGTC TGGGGGACGA GATCTGCAAG CAGCCCTGCA CCACCTCCAG AGCCAACTGT 
GGCCACCCGT GTATGGCACC CTGCCACCTC AGCTTGCCCT GCCCAGTGAC CACCTGCAAG 
GCCAAGATAG AGCTGCAGTG TGAATGTGGA CGAAGAAAAG AAATCATGGT TTGCTCAGAA 
GCATCCAGTA CTTATCAAAG AATAGCTGCT ATTTCCATGG CCTCCAAAAT AACAGACATG 
CAGCTTGGAG ATTCAGTGGA GATCAGCAAG TTGATTACCA AGAAGGAAAT TCACCAAGCC 
AGGCTGGAGT GTGATGAGGA ATGTTCAGCC TTGGAAAGGA AAAAGAGGTT AGCAGAGGCA 
TTTCATATCA GTGATGATTC TGATCCTTTC AACGTACGTT CTTCAGGGTC AAAATTCAGT 
GACAGTTTGA AAGAAGATGC TAGGAAGGAC TTAAGGTTTG TCAGCGACAT TGAGAAGGAA 
ATGGAAGCCC TTGTGGAGGC TGTGAATAAG GGGAAAAGTA GTAAGAAGAG CCACTGCTTC 
CCTCCCATGA ACAGAGACCA CCGCCGGATC ATCCACGATC TGGCCCAGGT GTACGGCCTG 
GAGAGCGTGA GCTATGACAG TGAGCCAAAG CGCAACGTCG TGGTCACTGC GGTCAGAGGG 
AAGTCCATTT GTCCTTCTAC CACACTGACA GGTGTACTCG AAAAGGAGAA TCAGTCACGG 
CCTCCGCCAC CGATTGCTCA TCACAGACAG ACAGACAAGA ATCCTGGGAG CAGCAATTTA 
CAGAAGATAG CGAAGGAGCC CGTAATTGAC TACTTTGATG TCCAGGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005210324.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210381.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

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CloneID	OBa22218
Clone ID Related Accession (Same CDS sequence)	XM_005210324.3
Accession Version	XM_005210324.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210324.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGCA GATGCTACAT TTATGTGCGA CAAGAGGTGT 
AACAAGAAGC GGTTATGTGG ACGGCATAAA TGTAATGAGA TATGCTGTGT GGATAAGGAG 
CACAAGTGTC CTTTGATTTG TGGGAGGAAA CTCCGCTGTG GCCTTCATAG ATGTGAAGAA 
CCTTGTCATC GTGGAAACTG CCAGACGTGC TGGCAAGCTA GTTTTGATGA ATTAACCTGC 
CACTGTGGTG CATCAGTGAT CTACCCTCCA GTTCCCTGTG GCACCAGGCC CCCTGAGTGT 
ACCCAGACCT GTGCCAGAGT CCATGAATGT GACCATCCAG TGTATCATTC ATGTCATAGT 
GAGGACAAGT GCCCTCCTTG TACTTTCCTC ACCCAGAAGT GGTGCATGGG CAAACACGAG 
TTACGAAGCA ACATTCCCTG TCACCTGGTT GATATCTCTT GTGGATTACC CTGCGGTGCC 
ACACTACCAT GTGGGATGCA CAAGTGTCAG AGGCTCTGTC ACAAAGGAGA ATGTCTGGGG 
GACGAGATCT GCAAGCAGCC CTGCACCACC TCCAGAGCCA ACTGTGGCCA CCCGTGTATG 
GCACCCTGCC ACCTCAGCTT GCCCTGCCCA GTGACCACCT GCAAGGCCAA GATAGAGCTG 
CAGTGTGAAT GTGGACGAAG AAAAGAAATC ATGGTTTGCT CAGAAGCATC CAGTACTTAT 
CAAAGAATAG CTGCTATTTC CATGGCCTCC AAAATAACAG ACATGCAGCT TGGAGATTCA 
GTGGAGATCA GCAAGTTGAT TACCAAGAAG GAAATTCACC AAGCCAGGCT GGAGTGTGAT 
GAGGAATGTT CAGCCTTGGA AAGGAAAAAG AGGTTAGCAG AGGCATTTCA TATCAGTGAT 
GATTCTGATC CTTTCAACGT ACGTTCTTCA GGGTCAAAAT TCAGTGACAG TTTGAAAGAA 
GATGCTAGGA AGGACTTAAG GTTTGTCAGC GACATTGAGA AGGAAATGGA AGCCCTTGTG 
GAGGCTGTGA ATAAGGGGAA AAGTAGTAAG AAGAGCCACT GCTTCCCTCC CATGAACAGA 
GACCACCGCC GGATCATCCA CGATCTGGCC CAGGTGTACG GCCTGGAGAG CGTGAGCTAT 
GACAGTGAGC CAAAGCGCAA CGTCGTGGTC ACTGCGGTCA GAGGGAAGTC CATTTGTCCT 
TCTACCACAC TGACAGGTGT ACTCGAAAAG GAGAATCAGT CACGGCCTCC GCCACCGATT 
GCTCATCACA GACAGACAGA CAAGAATCCT GGGAGCAGCA ATTTACAGAA GATAGCGAAG 
GAGCCCGTAA TTGACTACTT TGATGTCCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210381.1
CDS	127..3522
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEGMTGKGCDGTSWGEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005210324.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210324.3

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGCA GATGCTACAT TTATGTGCGA CAAGAGGTGT 
AACAAGAAGC GGTTATGTGG ACGGCATAAA TGTAATGAGA TATGCTGTGT GGATAAGGAG 
CACAAGTGTC CTTTGATTTG TGGGAGGAAA CTCCGCTGTG GCCTTCATAG ATGTGAAGAA 
CCTTGTCATC GTGGAAACTG CCAGACGTGC TGGCAAGCTA GTTTTGATGA ATTAACCTGC 
CACTGTGGTG CATCAGTGAT CTACCCTCCA GTTCCCTGTG GCACCAGGCC CCCTGAGTGT 
ACCCAGACCT GTGCCAGAGT CCATGAATGT GACCATCCAG TGTATCATTC ATGTCATAGT 
GAGGACAAGT GCCCTCCTTG TACTTTCCTC ACCCAGAAGT GGTGCATGGG CAAACACGAG 
TTACGAAGCA ACATTCCCTG TCACCTGGTT GATATCTCTT GTGGATTACC CTGCGGTGCC 
ACACTACCAT GTGGGATGCA CAAGTGTCAG AGGCTCTGTC ACAAAGGAGA ATGTCTGGGG 
GACGAGATCT GCAAGCAGCC CTGCACCACC TCCAGAGCCA ACTGTGGCCA CCCGTGTATG 
GCACCCTGCC ACCTCAGCTT GCCCTGCCCA GTGACCACCT GCAAGGCCAA GATAGAGCTG 
CAGTGTGAAT GTGGACGAAG AAAAGAAATC ATGGTTTGCT CAGAAGCATC CAGTACTTAT 
CAAAGAATAG CTGCTATTTC CATGGCCTCC AAAATAACAG ACATGCAGCT TGGAGATTCA 
GTGGAGATCA GCAAGTTGAT TACCAAGAAG GAAATTCACC AAGCCAGGCT GGAGTGTGAT 
GAGGAATGTT CAGCCTTGGA AAGGAAAAAG AGGTTAGCAG AGGCATTTCA TATCAGTGAT 
GATTCTGATC CTTTCAACGT ACGTTCTTCA GGGTCAAAAT TCAGTGACAG TTTGAAAGAA 
GATGCTAGGA AGGACTTAAG GTTTGTCAGC GACATTGAGA AGGAAATGGA AGCCCTTGTG 
GAGGCTGTGA ATAAGGGGAA AAGTAGTAAG AAGAGCCACT GCTTCCCTCC CATGAACAGA 
GACCACCGCC GGATCATCCA CGATCTGGCC CAGGTGTACG GCCTGGAGAG CGTGAGCTAT 
GACAGTGAGC CAAAGCGCAA CGTCGTGGTC ACTGCGGTCA GAGGGAAGTC CATTTGTCCT 
TCTACCACAC TGACAGGTGT ACTCGAAAAG GAGAATCAGT CACGGCCTCC GCCACCGATT 
GCTCATCACA GACAGACAGA CAAGAATCCT GGGAGCAGCA ATTTACAGAA GATAGCGAAG 
GAGCCCGTAA TTGACTACTT TGATGTCCAG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005210325.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210382.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

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CloneID	OBa22219
Clone ID Related Accession (Same CDS sequence)	XM_005210325.3
Accession Version	XM_005210325.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3393bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210325.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGAT GCTACATTTA TGTGCGACAA GAGGTGTAAC 
AAGAAGCGGT TATGTGGACG GCATAAATGT AATGAGATAT GCTGTGTGGA TAAGGAGCAC 
AAGTGTCCTT TGATTTGTGG GAGGAAACTC CGCTGTGGCC TTCATAGATG TGAAGAACCT 
TGTCATCGTG GAAACTGCCA GACGTGCTGG CAAGCTAGTT TTGATGAATT AACCTGCCAC 
TGTGGTGCAT CAGTGATCTA CCCTCCAGTT CCCTGTGGCA CCAGGCCCCC TGAGTGTACC 
CAGACCTGTG CCAGAGTCCA TGAATGTGAC CATCCAGTGT ATCATTCATG TCATAGTGAG 
GACAAGTGCC CTCCTTGTAC TTTCCTCACC CAGAAGTGGT GCATGGGCAA ACACGAGTTA 
CGAAGCAACA TTCCCTGTCA CCTGGTTGAT ATCTCTTGTG GATTACCCTG CGGTGCCACA 
CTACCATGTG GGATGCACAA GTGTCAGAGG CTCTGTCACA AAGGAGAATG TCTGGGGGAC 
GAGATCTGCA AGCAGCCCTG CACCACCTCC AGAGCCAACT GTGGCCACCC GTGTATGGCA 
CCCTGCCACC TCAGCTTGCC CTGCCCAGTG ACCACCTGCA AGGCCAAGAT AGAGCTGCAG 
TGTGAATGTG GACGAAGAAA AGAAATCATG GTTTGCTCAG AAGCATCCAG TACTTATCAA 
AGAATAGCTG CTATTTCCAT GGCCTCCAAA ATAACAGACA TGCAGCTTGG AGATTCAGTG 
GAGATCAGCA AGTTGATTAC CAAGAAGGAA ATTCACCAAG CCAGGCTGGA GTGTGATGAG 
GAATGTTCAG CCTTGGAAAG GAAAAAGAGG TTAGCAGAGG CATTTCATAT CAGTGATGAT 
TCTGATCCTT TCAACGTACG TTCTTCAGGG TCAAAATTCA GTGACAGTTT GAAAGAAGAT 
GCTAGGAAGG ACTTAAGGTT TGTCAGCGAC ATTGAGAAGG AAATGGAAGC CCTTGTGGAG 
GCTGTGAATA AGGGGAAAAG TAGTAAGAAG AGCCACTGCT TCCCTCCCAT GAACAGAGAC 
CACCGCCGGA TCATCCACGA TCTGGCCCAG GTGTACGGCC TGGAGAGCGT GAGCTATGAC 
AGTGAGCCAA AGCGCAACGT CGTGGTCACT GCGGTCAGAG GGAAGTCCAT TTGTCCTTCT 
ACCACACTGA CAGGTGTACT CGAAAAGGAG AATCAGTCAC GGCCTCCGCC ACCGATTGCT 
CATCACAGAC AGACAGACAA GAATCCTGGG AGCAGCAATT TACAGAAGAT AGCGAAGGAG 
CCCGTAATTG ACTACTTTGA TGTCCAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210382.1
CDS	127..3519
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEGMTGKGCDGTSWGEDATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005210325.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210325.3

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGGTAT GACTGGGAAG 
GGGTGTGATG GAACTTCTTG GGGTGAAGAT GCTACATTTA TGTGCGACAA GAGGTGTAAC 
AAGAAGCGGT TATGTGGACG GCATAAATGT AATGAGATAT GCTGTGTGGA TAAGGAGCAC 
AAGTGTCCTT TGATTTGTGG GAGGAAACTC CGCTGTGGCC TTCATAGATG TGAAGAACCT 
TGTCATCGTG GAAACTGCCA GACGTGCTGG CAAGCTAGTT TTGATGAATT AACCTGCCAC 
TGTGGTGCAT CAGTGATCTA CCCTCCAGTT CCCTGTGGCA CCAGGCCCCC TGAGTGTACC 
CAGACCTGTG CCAGAGTCCA TGAATGTGAC CATCCAGTGT ATCATTCATG TCATAGTGAG 
GACAAGTGCC CTCCTTGTAC TTTCCTCACC CAGAAGTGGT GCATGGGCAA ACACGAGTTA 
CGAAGCAACA TTCCCTGTCA CCTGGTTGAT ATCTCTTGTG GATTACCCTG CGGTGCCACA 
CTACCATGTG GGATGCACAA GTGTCAGAGG CTCTGTCACA AAGGAGAATG TCTGGGGGAC 
GAGATCTGCA AGCAGCCCTG CACCACCTCC AGAGCCAACT GTGGCCACCC GTGTATGGCA 
CCCTGCCACC TCAGCTTGCC CTGCCCAGTG ACCACCTGCA AGGCCAAGAT AGAGCTGCAG 
TGTGAATGTG GACGAAGAAA AGAAATCATG GTTTGCTCAG AAGCATCCAG TACTTATCAA 
AGAATAGCTG CTATTTCCAT GGCCTCCAAA ATAACAGACA TGCAGCTTGG AGATTCAGTG 
GAGATCAGCA AGTTGATTAC CAAGAAGGAA ATTCACCAAG CCAGGCTGGA GTGTGATGAG 
GAATGTTCAG CCTTGGAAAG GAAAAAGAGG TTAGCAGAGG CATTTCATAT CAGTGATGAT 
TCTGATCCTT TCAACGTACG TTCTTCAGGG TCAAAATTCA GTGACAGTTT GAAAGAAGAT 
GCTAGGAAGG ACTTAAGGTT TGTCAGCGAC ATTGAGAAGG AAATGGAAGC CCTTGTGGAG 
GCTGTGAATA AGGGGAAAAG TAGTAAGAAG AGCCACTGCT TCCCTCCCAT GAACAGAGAC 
CACCGCCGGA TCATCCACGA TCTGGCCCAG GTGTACGGCC TGGAGAGCGT GAGCTATGAC 
AGTGAGCCAA AGCGCAACGT CGTGGTCACT GCGGTCAGAG GGAAGTCCAT TTGTCCTTCT 
ACCACACTGA CAGGTGTACT CGAAAAGGAG AATCAGTCAC GGCCTCCGCC ACCGATTGCT 
CATCACAGAC AGACAGACAA GAATCCTGGG AGCAGCAATT TACAGAAGAT AGCGAAGGAG 
CCCGTAATTG ACTACTTTGA TGTCCAGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFX1 ( XM_005210326.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210383.1 Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa22220
Clone ID Related Accession (Same CDS sequence)	XM_005210326.3
Accession Version	XM_005210326.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	transcriptional repressor NF-X1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104127.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGCGACA AGAGGTGTAA CAAGAAGCGG TTATGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGTCCT TTGATTTGTG GGAGGAAACT CCGCTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGAAACTGCC AGACGTGCTG GCAAGCTAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGC 
ACCAGGCCCC CTGAGTGTAC CCAGACCTGT GCCAGAGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCAT GTCATAGTGA GGACAAGTGC CCTCCTTGTA CTTTCCTCAC CCAGAAGTGG 
TGCATGGGCA AACACGAGTT ACGAAGCAAC ATTCCCTGTC ACCTGGTTGA TATCTCTTGT 
GGATTACCCT GCGGTGCCAC ACTACCATGT GGGATGCACA AGTGTCAGAG GCTCTGTCAC 
AAAGGAGAAT GTCTGGGGGA CGAGATCTGC AAGCAGCCCT GCACCACCTC CAGAGCCAAC 
TGTGGCCACC CGTGTATGGC ACCCTGCCAC CTCAGCTTGC CCTGCCCAGT GACCACCTGC 
AAGGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AAGAAATCAT GGTTTGCTCA 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCCAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAGATCAGC AAGTTGATTA CCAAGAAGGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAATGTTCA GCCTTGGAAA GGAAAAAGAG GTTAGCAGAG 
GCATTTCATA TCAGTGATGA TTCTGATCCT TTCAACGTAC GTTCTTCAGG GTCAAAATTC 
AGTGACAGTT TGAAAGAAGA TGCTAGGAAG GACTTAAGGT TTGTCAGCGA CATTGAGAAG 
GAAATGGAAG CCCTTGTGGA GGCTGTGAAT AAGGGGAAAA GTAGTAAGAA GAGCCACTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ATCTGGCCCA GGTGTACGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAGCCA AAGCGCAACG TCGTGGTCAC TGCGGTCAGA 
GGGAAGTCCA TTTGTCCTTC TACCACACTG ACAGGTGTAC TCGAAAAGGA GAATCAGTCA 
CGGCCTCCGC CACCGATTGC TCATCACAGA CAGACAGACA AGAATCCTGG GAGCAGCAAT 
TTACAGAAGA TAGCGAAGGA GCCCGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210383.1
CDS	127..3480
Translation	MAEAPPVSGAFKFNTDAAEFIPQERKNSGLYCGSQRRLDSNRIGRRNYSSPPPCHLSRQISYDDISAVHQHSFYPSGSKPKSQQTSFQSSVTNKSLKNHGLQNQPWQKLRSEKQHIRVKRAQGLIDQTSDAPGLENVAKSESGTNLREHSPSESEKEIVGADPRGAKPKKATQFIYSYGRGPKVKGKLKSEWGHRMTPKPEEAGPENTKPAGVIHPDSSDASSRKVVADGARRGEQRRHPQKRSPWEVEGARPRPGRNPPKQEGQRHTNAGSRDNMASIPKDDLNERPAKSTCDSGNLAVVSRSSRRADPEKCAVRRQDPQVASFPRGKQNHMLKNVETHTGSLIEQLTTEKYECMVCCELVRVTAPVWSCQSCYHVFHLNCIKKWARSPASQADGQSGWRCPACQNVSAHVPNTYTCFCGKVKNPEWSRNEIPHSCGEVCRRKQPGQDCPHSCNLLCHPGPCPPCPAFMTKTCECGRTRHTVRCGQAVSVHCSNPCDNILNCGQHHCAELCHGGPCQPCRVILNQVCYCGSTSRDVLCGTDIGKSDGFGDFGCLKICGKDLKCGNHTCSQVCHPQPCQPCPRLPQLVRYCPCGQTPLSQLLELGSSGRKTCMDPVPSCGKVCGKPLPCGSLDFIHTCEKLCHEGDCGPCSRTSVISCRCSFRTKELPCTSLKSEADATFMCDKRCNKKRLCGRHKCNEICCVDKEHKCPLICGRKLRCGLHRCEEPCHRGNCQTCWQASFDELTCHCGASVIYPPVPCGTRPPECTQTCARVHECDHPVYHSCHSEDKCPPCTFLTQKWCMGKHELRSNIPCHLVDISCGLPCGATLPCGMHKCQRLCHKGECLGDEICKQPCTTSRANCGHPCMAPCHLSLPCPVTTCKAKIELQCECGRRKEIMVCSEASSTYQRIAAISMASKITDMQLGDSVEISKLITKKEIHQARLECDEECSALERKKRLAEAFHISDDSDPFNVRSSGSKFSDSLKEDARKDLRFVSDIEKEMEALVEAVNKGKSSKKSHCFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAVRGKSICPSTTLTGVLEKENQSRPPPPIAHHRQTDKNPGSSNLQKIAKEPVIDYFDVQD

Target ORF information:

RefSeq Version	XM_005210326.3
Organism	Bos taurus(cattle)
Definition	Bos taurus nuclear transcription factor, X-box binding 1 (NFX1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210326.3

ORF Insert Sequence:

ATGGCGGAGG CGCCTCCTGT CTCAGGTGCT TTTAAGTTCA ATACGGATGC TGCTGAGTTC 
ATTCCTCAGG AGAGAAAAAA TTCTGGTCTG TACTGTGGGA GTCAAAGGAG ACTAGATTCT 
AATAGAATTG GTAGAAGAAA TTACAGTTCA CCACCTCCGT GTCACCTTTC CAGGCAAATC 
TCTTATGATG ACATCTCTGC TGTTCATCAG CACAGTTTTT ATCCTTCAGG AAGCAAACCT 
AAGAGTCAGC AGACTTCTTT CCAGTCCTCT GTTACTAACA AATCTCTCAA GAACCATGGC 
CTTCAGAATC AACCTTGGCA GAAATTGAGG AGTGAAAAGC AGCATATCAG AGTCAAGAGA 
GCACAGGGCC TCATTGACCA GACCTCAGAT GCACCTGGCC TAGAAAACGT GGCCAAGTCA 
GAGAGTGGGA CAAACCTCAG AGAGCACAGT CCTTCCGAGA GTGAGAAGGA AATTGTCGGT 
GCAGATCCAA GGGGAGCAAA ACCCAAGAAA GCAACCCAAT TTATATACAG CTATGGGAGA 
GGACCAAAAG TCAAGGGGAA ACTCAAAAGT GAGTGGGGTC ACAGAATGAC TCCCAAACCT 
GAGGAGGCAG GACCTGAAAA CACCAAACCA GCAGGGGTTA TCCACCCTGA CTCTTCAGAT 
GCTTCCTCGA GAAAAGTGGT AGCGGATGGG GCCAGACGCG GTGAGCAGAG AAGACACCCA 
CAGAAAAGGT CTCCCTGGGA AGTGGAGGGA GCCAGGCCAC GACCAGGCAG GAACCCACCA 
AAACAGGAGG GCCAACGGCA TACAAACGCA GGGTCCAGAG ACAACATGGC CTCCATTCCA 
AAGGATGATC TTAATGAGAG GCCAGCAAAA TCCACCTGTG ACAGTGGGAA CTTGGCAGTC 
GTCAGCAGGT CTTCTAGAAG GGCTGACCCA GAGAAATGTG CTGTAAGGAG GCAGGATCCC 
CAGGTAGCAT CTTTTCCCCG AGGCAAACAG AACCACATGC TAAAGAATGT GGAAACACAC 
ACAGGCTCTC TGATTGAACA GCTCACCACA GAAAAATACG AGTGCATGGT GTGCTGTGAA 
TTGGTTCGTG TCACAGCCCC AGTGTGGAGT TGTCAGAGCT GTTACCATGT GTTTCATTTG 
AACTGCATAA AGAAATGGGC CAGGTCTCCA GCATCTCAAG CAGATGGCCA GAGTGGTTGG 
AGGTGCCCCG CATGTCAGAA TGTTTCTGCA CATGTTCCTA ATACCTATAC TTGTTTCTGT 
GGCAAGGTAA AGAATCCTGA ATGGAGCAGA AATGAAATTC CACATAGTTG TGGTGAGGTC 
TGTAGAAGGA AACAGCCTGG CCAGGACTGC CCACATTCCT GTAACCTTCT GTGCCATCCA 
GGACCTTGCC CACCCTGCCC TGCCTTTATG ACTAAAACAT GTGAATGTGG ACGGACCAGG 
CACACAGTTC GCTGCGGTCA GGCTGTCTCA GTTCACTGTT CAAACCCTTG TGACAATATT 
TTGAATTGTG GTCAGCACCA CTGTGCTGAG CTGTGCCATG GGGGTCCATG CCAACCTTGT 
CGGGTCATAC TGAACCAGGT ATGCTACTGT GGCAGCACCT CCCGAGATGT GTTATGTGGA 
ACAGACATAG GAAAGTCTGA TGGATTTGGG GACTTTGGCT GTTTAAAGAT ATGTGGCAAG 
GACTTGAAAT GTGGGAACCA TACGTGTTCT CAAGTGTGTC ACCCTCAGCC CTGCCAGCCG 
TGCCCACGGC TTCCCCAGCT GGTGCGCTAT TGCCCTTGTG GGCAAACTCC TCTCAGCCAG 
TTGCTGGAAC TTGGAAGTAG TGGTCGGAAG ACATGCATGG ATCCTGTGCC TTCATGTGGA 
AAAGTGTGTG GCAAGCCTCT GCCATGTGGT TCCCTAGATT TCATTCATAC CTGTGAAAAG 
CTCTGTCATG AAGGAGACTG TGGACCATGC TCTCGCACAT CAGTTATTTC CTGCAGATGC 
TCTTTCAGAA CAAAGGAGCT TCCCTGTACC AGTCTCAAAA GTGAAGCAGA TGCTACATTT 
ATGTGCGACA AGAGGTGTAA CAAGAAGCGG TTATGTGGAC GGCATAAATG TAATGAGATA 
TGCTGTGTGG ATAAGGAGCA CAAGTGTCCT TTGATTTGTG GGAGGAAACT CCGCTGTGGC 
CTTCATAGAT GTGAAGAACC TTGTCATCGT GGAAACTGCC AGACGTGCTG GCAAGCTAGT 
TTTGATGAAT TAACCTGCCA CTGTGGTGCA TCAGTGATCT ACCCTCCAGT TCCCTGTGGC 
ACCAGGCCCC CTGAGTGTAC CCAGACCTGT GCCAGAGTCC ATGAATGTGA CCATCCAGTG 
TATCATTCAT GTCATAGTGA GGACAAGTGC CCTCCTTGTA CTTTCCTCAC CCAGAAGTGG 
TGCATGGGCA AACACGAGTT ACGAAGCAAC ATTCCCTGTC ACCTGGTTGA TATCTCTTGT 
GGATTACCCT GCGGTGCCAC ACTACCATGT GGGATGCACA AGTGTCAGAG GCTCTGTCAC 
AAAGGAGAAT GTCTGGGGGA CGAGATCTGC AAGCAGCCCT GCACCACCTC CAGAGCCAAC 
TGTGGCCACC CGTGTATGGC ACCCTGCCAC CTCAGCTTGC CCTGCCCAGT GACCACCTGC 
AAGGCCAAGA TAGAGCTGCA GTGTGAATGT GGACGAAGAA AAGAAATCAT GGTTTGCTCA 
GAAGCATCCA GTACTTATCA AAGAATAGCT GCTATTTCCA TGGCCTCCAA AATAACAGAC 
ATGCAGCTTG GAGATTCAGT GGAGATCAGC AAGTTGATTA CCAAGAAGGA AATTCACCAA 
GCCAGGCTGG AGTGTGATGA GGAATGTTCA GCCTTGGAAA GGAAAAAGAG GTTAGCAGAG 
GCATTTCATA TCAGTGATGA TTCTGATCCT TTCAACGTAC GTTCTTCAGG GTCAAAATTC 
AGTGACAGTT TGAAAGAAGA TGCTAGGAAG GACTTAAGGT TTGTCAGCGA CATTGAGAAG 
GAAATGGAAG CCCTTGTGGA GGCTGTGAAT AAGGGGAAAA GTAGTAAGAA GAGCCACTGC 
TTCCCTCCCA TGAACAGAGA CCACCGCCGG ATCATCCACG ATCTGGCCCA GGTGTACGGC 
CTGGAGAGCG TGAGCTATGA CAGTGAGCCA AAGCGCAACG TCGTGGTCAC TGCGGTCAGA 
GGGAAGTCCA TTTGTCCTTC TACCACACTG ACAGGTGTAC TCGAAAAGGA GAATCAGTCA 
CGGCCTCCGC CACCGATTGC TCATCACAGA CAGACAGACA AGAATCCTGG GAGCAGCAAT 
TTACAGAAGA TAGCGAAGGA GCCCGTAATT GACTACTTTG ATGTCCAGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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NFX1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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