ARHGEF12 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017017422.1	XP_016872911.1	rho guanine nucleotide exchange factor 12 isoform X4
NM_001198665.1	NP_001185594.1	rho guanine nucleotide exchange factor 12 isoform 2
XM_017017421.1	XP_016872910.1	rho guanine nucleotide exchange factor 12 isoform X1
NM_015313.3	NP_056128.1	rho guanine nucleotide exchange factor 12 isoform 1
NM_001301084.1	NP_001288013.1	rho guanine nucleotide exchange factor 12 isoform 3
NM_015313.2	NP_056128.1	rho guanine nucleotide exchange factor 12 isoform 1
XM_006718805.3	XP_006718868.1	rho guanine nucleotide exchange factor 12 isoform X2
XM_017017420.1	XP_016872909.1	rho guanine nucleotide exchange factor 12 isoform X3
XM_017017422.2	XP_016872911.1	rho guanine nucleotide exchange factor 12 isoform X4
XM_011542720.2	XP_011541022.1	rho guanine nucleotide exchange factor 12 isoform X1
NM_001198665.2	NP_001185594.1	rho guanine nucleotide exchange factor 12 isoform 2
NM_001301084.2	NP_001288013.1	rho guanine nucleotide exchange factor 12 isoform 3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21676	NM_015313.3 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu92069	XM_017017420.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu92070	XM_017017422.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu07698	NM_001198665.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45709	NM_001301084.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu45707	XM_006718805.3 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45709	XM_011542720.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu45709	XM_017017421.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu21676	NM_015313.2	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$579.50-$811.30 $1159.00
OHu92070	XM_017017422.1	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu07698	NM_001198665.1	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu45709	NM_001301084.1	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
Hide Old Accession Versions >>

ARHGEF12 ( NM_015313.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056128.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21676
Clone ID Related Accession (Same CDS sequence)	NM_015313.3 , NM_015313.2
Accession Version	NM_015313.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001150.5, BC063117.1, AF180681.1, AK304316.1 and AP000758.5. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_015313.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF180681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000397843.7/ ENSP00000380942.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056128.1
CDS	832..5466
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_015313.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015313.3

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( NM_015313.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_056128.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21676
Clone ID Related Accession (Same CDS sequence)	NM_015313.3 , NM_015313.2
Accession Version	NM_015313.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC063117.1, AF180681.1, AK304316.1 and AP000758.5. This sequence is a reference standard in the RefSeqGene project. On Nov 2, 2010 this sequence version replaced NM_015313.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF180681.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056128.1
CDS	336..4970
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_015313.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015313.2

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT 
CGGCCCAGTA GTGACAATGC AGATAGTCCC AAGAGTGGCC CAAAAGAGAG AATTTATCTA 
GAGGAAAACC CAGAGAAAAG TGAAACAATT CAGGACACTG ACACTCAATC ACTTGTCGGA 
AGTCCCTCAA CCCGTATAGC ACCTCATATT ATTGGAGCAG AAGATGATGA TTTTGGTACT 
GAACATGAAC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT ACTAAAATCT 
CGCCCGGCTC ATTTGGCTGT TTTCTTACAC CATGTAGTTT CACAATTTGA CCCTGCGACT 
TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAACATACCA ATTCCAAAGA AACTCGTCGC 
ATCTTCCTTG AGTTTCATCA GTTCTTTCTA GATCGATCAG CACACCTGAA AGTTTCTGTT 
CCTGATGAAA TGTCTGCAGA TCTAGAAAAG AGAAGACCTG AGCTCATTCC TGAGGATCTG 
CATCGCCACT ATATCCAAAC TATGCAAGAA AGAGTCCATC CAGAAGTTCA AAGGCACTTA 
GAAGATTTTC GGCAGAAACG TAGTATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA 
CTTGATGCAG AGCGAGACAA GGACCGATTG ACTTTGGAGA AGGAGCGGAC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTATTG ATGACTGCTC AGGCTGTAGA GGAAGATAAG 
AGCTCCACCA TGCAGTATGT TATTCTCATG TATATGAAGC ATTTGGGAGT AAAAGTGAAA 
GAGCCTCGAA ATTTGGAGCA CAAACGGGGT CGGATTGGAT TTCTTCCCAA AATCAAGCAA 
AGTATGAAGA AAGATAAAGA AGGGGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATC 
CTGGGACCCC CACGGAGACC AAGCCGTCAT GACAACAGTG CAATTGGCAG AGCCATGGAA 
CTACAGAAGG CGCGCCACCC TAAGCACTTA TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGACTCTG CCAAGTTGCG CCAGAGTGGG TTAGCAAATG AAGGAACAGA CGCTGGATAC 
CTGCCTGCCA ATTCCATGTC TTCTGTAGCT TCAGGGGCCT CTTTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAT CAGCACCTGG AGACACCTTA 
GATGGCACAC CTCGTACTCT CAATACTGTC TTTGATTTCC CACCACCTCC ATTAGACCAA 
GTGCAGGAGG AGGAATGTGA AGTAGAAAGG GTGACTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGTGTAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA ATTTGAAAAT 
GACTTAGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GTCGAGAAGT GTTACTGGGA 
CTAAAACCTT GTGAAATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACACTGAA GGTTCTTGAT CAAGTGTTCT ATCAGCGAGT ATCCAGAGAA 
GGAATTCTGT CACCCTCAGA GCTACGGAAA ATTTTTTCAA ACTTGGAAGA TATTCTTCAA 
CTTCATATTG GATTGAATGA ACAAATGAAG GCTGTTCGAA AGAGAAATGA GACCTCTGTT 
ATCGATCAGA TTGGGGAAGA TTTGCTGACA TGGTTCAGCG GACCAGGAGA GGAGAAATTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAATCT 
CGTCAGAAAA AGGATTCTCG ATTTCAGACT TTTGTGCAAG ATGCTGAAAG TAATCCACTG 
TGTCGTCGTC TTCAACTGAA GGATATTATT CCCACTCAAA TGCAAAGGCT TACTAAGTAC 
CCACTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAAGGAG 
GCAGAAAACA AGCAGCGCCT AGAAGATTAT CAGCGTCGCC TTGATACCTC CAGCCTGAAG 
TTGTCAGAGT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCATGAAG GGCCATTGGT TTGGAAGGTG AATAGAGATA AAACTATTGA TTTATACACG 
TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTTTTAAGG 
TGTCATAGTA AGATTCTGGC ATCTACAGCT GATAGCAAAC ACACGTTTAG CCCTGTCATT 
AAGTTGAGTA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATT 
TCCATGTCAG ACAATGGCGC TCAGATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAGCA ATCCACAAAG 
CCAATTCCAT TACCACAGTC AACACCTGGC GAAGGAGATA ATGATGAAGA AGATCCTTCA 
AAATTAAAAG AGGAGCAGCA TGGCATTTCA GTCACTGGTT TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AATCTACCTT AATATCGTCA AAACCTCAGT CTCATTCACT GAGTACCTCT 
GGGAAATCAG AGGTACGTGA TCTGTTTGTG GCTGAGAGAC AGTTTGCAAA GGAACAACAT 
ACAGATGGGA CACTAAAGGA AGTTGGAGAA GATTATCAAA TCGCAATCCC AGATTCACAC 
CTGCCTGTCT CAGAAGAACG GTGGGCATTG GATGCACTAA GAAATTTGGG TTTGTTGAAG 
CAGTTGCTGG TGCAACAGCT AGGTTTGACT GAGAAGAGCG TTCAGGAAGA CTGGCAACAT 
TTCCCAAGAT ACAGAACAGC CTCTCAGGGG CCGCAGACAG ACAGTGTCAT CCAGAACTCT 
GAAAATATTA AGGCCTATCA TTCTGGTGAA GGACATATGC CCTTTAGAAC TGGAACTGGT 
GACATTGCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTTTGCTCC ACGGGATTCA 
GTGGGACTGG CACCCCAGGA TAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTAC CATGGAGCCA GAAGGGGGTC TTGATGACAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GAGAATCCAT CAGAAGGTGA TGGAGCAGTT 
AACAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTATTTGAT CCTTGATGGC 
TATGACCCAG TGCAGGAGAG TTCCACAGAT GAGGAGGTTG CTTCCTCACT TACCCTGCAG 
CCCATGACAG GCATCCCTGC TGTGGAATCC ACCCACCAGC AGCAACATTC TCCTCAGAAT 
ACTCACTCCG ATGGGGCAAT TTCACCATTC ACCCCCGAAT TTCTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGTATTCCTG TTTTGAGATC CAGAGTCCCT CCTCTTGTGC AGATTCACAG 
AGCCAGATCA TGGAGTACAT TCATAAGATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_017017420.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872909.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

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CloneID	OHu92069
Clone ID Related Accession (Same CDS sequence)	XM_017017420.1
Accession Version	XM_017017420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4458bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872909.1
CDS	969..5426
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_017017420.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017420.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_017017422.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872911.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

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CloneID	OHu92070
Clone ID Related Accession (Same CDS sequence)	XM_017017422.1 , XM_017017422.2
Accession Version	XM_017017422.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872911.1
CDS	473..4678
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_017017422.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017422.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_017017422.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872911.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

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CloneID	OHu92070
Clone ID Related Accession (Same CDS sequence)	XM_017017422.1 , XM_017017422.2
Accession Version	XM_017017422.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017017422.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872911.1
CDS	472..4677
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_017017422.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017422.2

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( NM_001198665.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185594.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07698
Clone ID Related Accession (Same CDS sequence)	NM_001198665.1 , NM_001198665.2
Accession Version	NM_001198665.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC063117.1, AF180681.1, AK304316.1 and AP000758.5. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185594.1
CDS	336..4913
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_001198665.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198665.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( NM_001198665.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185594.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.

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CloneID	OHu07698
Clone ID Related Accession (Same CDS sequence)	NM_001198665.1 , NM_001198665.2
Accession Version	NM_001198665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP001150.5, BC063117.1, AF180681.1, AK304316.1 and AP000758.5. On Jun 1, 2019 this sequence version replaced NM_001198665.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185594.1
CDS	832..5409
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_001198665.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198665.2

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGGTCTTGTT CAGCGTTGCG TAATCATCCA GAAAGATGAC 
AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA 
GATGGAGCAG CCATGCGGGC TGGAGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA 
ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAGC TAATCAAATC TGGTTCCTAT 
GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT TGCCGACTCT 
GAAGTAGAGC CGTCAGTCAT TGGACATATG TCTCCCATCA TGACATCTCC TCATTCACCT 
GGAGCATCTG GGAATATGGA GAGAATCACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT 
GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAAATGT TACAGAAAGA ACAGGAACGG 
CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC 
CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT 
GCTCAGGATG GAGCTGTAGT TACACCCTCC AGACCTTTAG GGGACACCCT AACAGTCAGT 
GAGGCAGAAA CAGATCCTGG AGATGTACTG GGCAGGACTG ACTGTAGCAG TGGAGATGCT 
TCTCGGCCCA GTAGTGACAA TGCAGATAGT CCCAAGAGTG GCCCAAAAGA GAGAATTTAT 
CTAGAGGAAA ACCCAGAGAA AAGTGAAACA ATTCAGGACA CTGACACTCA ATCACTTGTC 
GGAAGTCCCT CAACCCGTAT AGCACCTCAT ATTATTGGAG CAGAAGATGA TGATTTTGGT 
ACTGAACATG AACAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTACTAAAA 
TCTCGCCCGG CTCATTTGGC TGTTTTCTTA CACCATGTAG TTTCACAATT TGACCCTGCG 
ACTTTGCTCT GTTATCTCTA TTCAGACCTG TATAAACATA CCAATTCCAA AGAAACTCGT 
CGCATCTTCC TTGAGTTTCA TCAGTTCTTT CTAGATCGAT CAGCACACCT GAAAGTTTCT 
GTTCCTGATG AAATGTCTGC AGATCTAGAA AAGAGAAGAC CTGAGCTCAT TCCTGAGGAT 
CTGCATCGCC ACTATATCCA AACTATGCAA GAAAGAGTCC ATCCAGAAGT TCAAAGGCAC 
TTAGAAGATT TTCGGCAGAA ACGTAGTATG GGACTGACCT TGGCTGAAAG CGAGCTGACT 
AAACTTGATG CAGAGCGAGA CAAGGACCGA TTGACTTTGG AGAAGGAGCG GACATGTGCA 
GAACAGATTG TTGCCAAAAT TGAAGAAGTA TTGATGACTG CTCAGGCTGT AGAGGAAGAT 
AAGAGCTCCA CCATGCAGTA TGTTATTCTC ATGTATATGA AGCATTTGGG AGTAAAAGTG 
AAAGAGCCTC GAAATTTGGA GCACAAACGG GGTCGGATTG GATTTCTTCC CAAAATCAAG 
CAAAGTATGA AGAAAGATAA AGAAGGGGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC 
ATCCTGGGAC CCCCACGGAG ACCAAGCCGT CATGACAACA GTGCAATTGG CAGAGCCATG 
GAACTACAGA AGGCGCGCCA CCCTAAGCAC TTATCCACAC CCTCATCTGT GAGTCCTGAA 
CCTCAGGACT CTGCCAAGTT GCGCCAGAGT GGGTTAGCAA ATGAAGGAAC AGACGCTGGA 
TACCTGCCTG CCAATTCCAT GTCTTCTGTA GCTTCAGGGG CCTCTTTTTC CCAGGAAGGA 
GGGAAAGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CATCAGCACC TGGAGACACC 
TTAGATGGCA CACCTCGTAC TCTCAATACT GTCTTTGATT TCCCACCACC TCCATTAGAC 
CAAGTGCAGG AGGAGGAATG TGAAGTAGAA AGGGTGACTG AACATGGGAC ACCAAAGCCC 
TTTCGAAAGT TTGACAGTGT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAATTTGAA 
AATGACTTAG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGTCGAGA AGTGTTACTG 
GGACTAAAAC CTTGTGAAAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAA 
AGAGCTCATG TTCGAACACT GAAGGTTCTT GATCAAGTGT TCTATCAGCG AGTATCCAGA 
GAAGGAATTC TGTCACCCTC AGAGCTACGG AAAATTTTTT CAAACTTGGA AGATATTCTT 
CAACTTCATA TTGGATTGAA TGAACAAATG AAGGCTGTTC GAAAGAGAAA TGAGACCTCT 
GTTATCGATC AGATTGGGGA AGATTTGCTG ACATGGTTCA GCGGACCAGG AGAGGAGAAA 
TTGAAACATG CTGCTGCTAC CTTTTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAA 
TCTCGTCAGA AAAAGGATTC TCGATTTCAG ACTTTTGTGC AAGATGCTGA AAGTAATCCA 
CTGTGTCGTC GTCTTCAACT GAAGGATATT ATTCCCACTC AAATGCAAAG GCTTACTAAG 
TACCCACTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAACAGA AAGGGAGAAG 
GTGAAGAAAG CTGCAGATCA CTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAAG 
GAGGCAGAAA ACAAGCAGCG CCTAGAAGAT TATCAGCGTC GCCTTGATAC CTCCAGCCTG 
AAGTTGTCAG AGTACCCAAA TGTTGAAGAG CTCAGGAATT TGGATTTAAC AAAAAGGAAG 
ATGATTCATG AAGGGCCATT GGTTTGGAAG GTGAATAGAG ATAAAACTAT TGATTTATAC 
ACGTTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTTTTA 
AGGTGTCATA GTAAGATTCT GGCATCTACA GCTGATAGCA AACACACGTT TAGCCCTGTC 
ATTAAGTTGA GTACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTCGTC 
ATTTCCATGT CAGACAATGG CGCTCAGATT TATGAACTGG TGGCACAGAC AGTTTCTGAA 
AAGACTGTCT GGCAGGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA GCAATCCACA 
AAGCCAATTC CATTACCACA GTCAACACCT GGCGAAGGAG ATAATGATGA AGAAGATCCT 
TCAAAATTAA AAGAGGAGCA GCATGGCATT TCAGTCACTG GTTTGCAGAG TCCAGACAGA 
GATTTGGGAT TAGAATCTAC CTTAATATCG TCAAAACCTC AGTCTCATTC ACTGAGTACC 
TCTGGGAAAT CAGAGGTACG TGATCTGTTT GTGGCTGAGA GACAGTTTGC AAAGGAACAA 
CATACAGATG GGACACTAAA GGAAGTTGGA GAAGATTATC AAATCGCAAT CCCAGATTCA 
CACCTGCCTG TCTCAGAAGA ACGGTGGGCA TTGGATGCAC TAAGAAATTT GGGTTTGTTG 
AAGCAGTTGC TGGTGCAACA GCTAGGTTTG ACTGAGAAGA GCGTTCAGGA AGACTGGCAA 
CATTTCCCAA GATACAGAAC AGCCTCTCAG GGGCCGCAGA CAGACAGTGT CATCCAGAAC 
TCTGAAAATA TTAAGGCCTA TCATTCTGGT GAAGGACATA TGCCCTTTAG AACTGGAACT 
GGTGACATTG CAACTTGTTA CAGTCCACGG ACTTCAACTG AATCTTTTGC TCCACGGGAT 
TCAGTGGGAC TGGCACCCCA GGATAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG 
ATTATGACCC CAGAGATGCC TACCATGGAG CCAGAAGGGG GTCTTGATGA CAGTGGAGAG 
CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGAGAATC CATCAGAAGG TGATGGAGCA 
GTTAACAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTATTT GATCCTTGAT 
GGCTATGACC CAGTGCAGGA GAGTTCCACA GATGAGGAGG TTGCTTCCTC ACTTACCCTG 
CAGCCCATGA CAGGCATCCC TGCTGTGGAA TCCACCCACC AGCAGCAACA TTCTCCTCAG 
AATACTCACT CCGATGGGGC AATTTCACCA TTCACCCCCG AATTTCTGGT CCAGCAGCGC 
TGGGGAGCTA TGGAGTATTC CTGTTTTGAG ATCCAGAGTC CCTCCTCTTG TGCAGATTCA 
CAGAGCCAGA TCATGGAGTA CATTCATAAG ATAGAGGCTG ACCTTGAACA CTTAAAGAAG 
GTGGAGGAAA GTTACACCAT TCTTTGCCAA AGGCTGGCTG GATCAGCCCT CACAGACAAG 
CACTCAGATA AAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( NM_001301084.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288013.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.

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CloneID	OHu45709
Clone ID Related Accession (Same CDS sequence)	XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
Accession Version	NM_001301084.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-22
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC350360.1, BX647785.1, AP000659.4, AF180681.1 and AP000758.5. Transcript Variant: This variant (3) uses an alternate first exon and initiates translation at a downstream AUG compared to variant 1. Thd resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647785.1, SRR1660805.161593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288013.1
CDS	471..4796
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_001301084.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301084.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( NM_001301084.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001288013.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.

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CloneID	OHu45709
Clone ID Related Accession (Same CDS sequence)	XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
Accession Version	NM_001301084.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX647785.1, AP000659.4, AF180681.1 and AP000758.5. On May 31, 2019 this sequence version replaced NM_001301084.1. Transcript Variant: This variant (3) uses an alternate first exon and initiates translation at a downstream AUG compared to variant 1. Thd resulting isoform (3) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.129029.1, SRR1660805.161593.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001288013.1
CDS	438..4763
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_001301084.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301084.2

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_006718805.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006718868.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

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CloneID	OHu45707
Clone ID Related Accession (Same CDS sequence)	XM_006718805.3
Accession Version	XM_006718805.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718805.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT 
CGGCCCAGTA GTGACAATGC AGATGACACT CAATCACTTG TCGGAAGTCC CTCAACCCGT 
ATAGCACCTC ATATTATTGG AGCAGAAGAT GATGATTTTG GTACTGAACA TGAACAGATC 
AATGGACAGT GCAGCTGTTT CCAGAGCATT GAATTACTAA AATCTCGCCC GGCTCATTTG 
GCTGTTTTCT TACACCATGT AGTTTCACAA TTTGACCCTG CGACTTTGCT CTGTTATCTC 
TATTCAGACC TGTATAAACA TACCAATTCC AAAGAAACTC GTCGCATCTT CCTTGAGTTT 
CATCAGTTCT TTCTAGATCG ATCAGCACAC CTGAAAGTTT CTGTTCCTGA TGAAATGTCT 
GCAGATCTAG AAAAGAGAAG ACCTGAGCTC ATTCCTGAGG ATCTGCATCG CCACTATATC 
CAAACTATGC AAGAAAGAGT CCATCCAGAA GTTCAAAGGC ACTTAGAAGA TTTTCGGCAG 
AAACGTAGTA TGGGACTGAC CTTGGCTGAA AGCGAGCTGA CTAAACTTGA TGCAGAGCGA 
GACAAGGACC GATTGACTTT GGAGAAGGAG CGGACATGTG CAGAACAGAT TGTTGCCAAA 
ATTGAAGAAG TATTGATGAC TGCTCAGGCT GTAGAGGAAG ATAAGAGCTC CACCATGCAG 
TATGTTATTC TCATGTATAT GAAGCATTTG GGAGTAAAAG TGAAAGAGCC TCGAAATTTG 
GAGCACAAAC GGGGTCGGAT TGGATTTCTT CCCAAAATCA AGCAAAGTAT GAAGAAAGAT 
AAAGAAGGGG AAGAAAAAGG GAAGCGAAGA GGATTCCCCA GCATCCTGGG ACCCCCACGG 
AGACCAAGCC GTCATGACAA CAGTGCAATT GGCAGAGCCA TGGAACTACA GAAGGCGCGC 
CACCCTAAGC ACTTATCCAC ACCCTCATCT GTGAGTCCTG AACCTCAGGA CTCTGCCAAG 
TTGCGCCAGA GTGGGTTAGC AAATGAAGGA ACAGACGCTG GATACCTGCC TGCCAATTCC 
ATGTCTTCTG TAGCTTCAGG GGCCTCTTTT TCCCAGGAAG GAGGGAAAGA GAATGATACA 
GGATCAAAGC AAGTTGGAGA AACATCAGCA CCTGGAGACA CCTTAGATGG CACACCTCGT 
ACTCTCAATA CTGTCTTTGA TTTCCCACCA CCTCCATTAG ACCAAGTGCA GGAGGAGGAA 
TGTGAAGTAG AAAGGGTGAC TGAACATGGG ACACCAAAGC CCTTTCGAAA GTTTGACAGT 
GTAGCTTTTG GAGAAAGTCA AAGTGAGGAT GAACAATTTG AAAATGACTT AGAGACAGAT 
CCACCCAACT GGCAGCAGCT TGTTAGTCGA GAAGTGTTAC TGGGACTAAA ACCTTGTGAA 
ATCAAAAGAC AGGAAGTGAT TAATGAATTG TTCTACACTG AAAGAGCTCA TGTTCGAACA 
CTGAAGGTTC TTGATCAAGT GTTCTATCAG CGAGTATCCA GAGAAGGAAT TCTGTCACCC 
TCAGAGCTAC GGAAAATTTT TTCAAACTTG GAAGATATTC TTCAACTTCA TATTGGATTG 
AATGAACAAA TGAAGGCTGT TCGAAAGAGA AATGAGACCT CTGTTATCGA TCAGATTGGG 
GAAGATTTGC TGACATGGTT CAGCGGACCA GGAGAGGAGA AATTGAAACA TGCTGCTGCT 
ACCTTTTGCA GTAACCAACC TTTCGCCCTG GAAATGATCA AATCTCGTCA GAAAAAGGAT 
TCTCGATTTC AGACTTTTGT GCAAGATGCT GAAAGTAATC CACTGTGTCG TCGTCTTCAA 
CTGAAGGATA TTATTCCCAC TCAAATGCAA AGGCTTACTA AGTACCCACT TCTGTTGGAT 
AATATTGCCA AATACACAGA ATGGCCAACA GAAAGGGAGA AGGTGAAGAA AGCTGCAGAT 
CACTGTCGTC AGATCTTAAA TTATGTAAAT CAGGCTGTCA AGGAGGCAGA AAACAAGCAG 
CGCCTAGAAG ATTATCAGCG TCGCCTTGAT ACCTCCAGCC TGAAGTTGTC AGAGTACCCA 
AATGTTGAAG AGCTCAGGAA TTTGGATTTA ACAAAAAGGA AGATGATTCA TGAAGGGCCA 
TTGGTTTGGA AGGTGAATAG AGATAAAACT ATTGATTTAT ACACGTTGCT GCTGGAAGAC 
ATTCTTGTAT TGTTACAAAA GCAGGATGAT AGACTGGTTT TAAGGTGTCA TAGTAAGATT 
CTGGCATCTA CAGCTGATAG CAAACACACG TTTAGCCCTG TCATTAAGTT GAGTACAGTG 
TTGGTTCGAC AAGTGGCAAC AGATAACAAA GCTTTATTCG TCATTTCCAT GTCAGACAAT 
GGCGCTCAGA TTTATGAACT GGTGGCACAG ACAGTTTCTG AAAAGACTGT CTGGCAGGAC 
CTAATCTGTC GGATGGCTGC ATCAGTGAAG GAGCAATCCA CAAAGCCAAT TCCATTACCA 
CAGTCAACAC CTGGCGAAGG AGATAATGAT GAAGAAGATC CTTCAAAATT AAAAGAGGAG 
CAGCATGGCA TTTCAGTCAC TGGTTTGCAG AGTCCAGACA GAGATTTGGG ATTAGAATCT 
ACCTTAATAT CGTCAAAACC TCAGTCTCAT TCACTGAGTA CCTCTGGGAA ATCAGAGGTA 
CGTGATCTGT TTGTGGCTGA GAGACAGTTT GCAAAGGAAC AACATACAGA TGGGACACTA 
AAGGAAGTTG GAGAAGATTA TCAAATCGCA ATCCCAGATT CACACCTGCC TGTCTCAGAA 
GAACGGTGGG CATTGGATGC ACTAAGAAAT TTGGGTTTGT TGAAGCAGTT GCTGGTGCAA 
CAGCTAGGTT TGACTGAGAA GAGCGTTCAG GAAGACTGGC AACATTTCCC AAGATACAGA 
ACAGCCTCTC AGGGGCCGCA GACAGACAGT GTCATCCAGA ACTCTGAAAA TATTAAGGCC 
TATCATTCTG GTGAAGGACA TATGCCCTTT AGAACTGGAA CTGGTGACAT TGCAACTTGT 
TACAGTCCAC GGACTTCAAC TGAATCTTTT GCTCCACGGG ATTCAGTGGG ACTGGCACCC 
CAGGATAGCC AGGCAAGTAA CATTTTAGTA ATGGACCACA TGATTATGAC CCCAGAGATG 
CCTACCATGG AGCCAGAAGG GGGTCTTGAT GACAGTGGAG AGCACTTTTT TGATGCCCGT 
GAAGCACATA GTGATGAGAA TCCATCAGAA GGTGATGGAG CAGTTAACAA GGAAGAGAAG 
GATGTTAATT TACGCATCTC AGGAAACTAT TTGATCCTTG ATGGCTATGA CCCAGTGCAG 
GAGAGTTCCA CAGATGAGGA GGTTGCTTCC TCACTTACCC TGCAGCCCAT GACAGGCATC 
CCTGCTGTGG AATCCACCCA CCAGCAGCAA CATTCTCCTC AGAATACTCA CTCCGATGGG 
GCAATTTCAC CATTCACCCC CGAATTTCTG GTCCAGCAGC GCTGGGGAGC TATGGAGTAT 
TCCTGTTTTG AGATCCAGAG TCCCTCCTCT TGTGCAGATT CACAGAGCCA GATCATGGAG 
TACATTCATA AGATAGAGGC TGACCTTGAA CACTTAAAGA AGGTGGAGGA AAGTTACACC 
ATTCTTTGCC AAAGGCTGGC TGGATCAGCC CTCACAGACA AGCACTCAGA TAAAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006718868.1
CDS	695..5254
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERIASHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_006718805.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006718805.3

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCATTGCA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGCGTAA TCATCCAGAA AGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTTGC CGACTCTGAA 
GTAGAGCCGT CAGTCATTGG ACATATGTCT CCCATCATGA CATCTCCTCA TTCACCTGGA 
GCATCTGGGA ATATGGAGAG AATCACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTA 
CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ACCCTCCAGA CCTTTAGGGG ACACCCTAAC AGTCAGTGAG 
GCAGAAACAG ATCCTGGAGA TGTACTGGGC AGGACTGACT GTAGCAGTGG AGATGCTTCT 
CGGCCCAGTA GTGACAATGC AGATGACACT CAATCACTTG TCGGAAGTCC CTCAACCCGT 
ATAGCACCTC ATATTATTGG AGCAGAAGAT GATGATTTTG GTACTGAACA TGAACAGATC 
AATGGACAGT GCAGCTGTTT CCAGAGCATT GAATTACTAA AATCTCGCCC GGCTCATTTG 
GCTGTTTTCT TACACCATGT AGTTTCACAA TTTGACCCTG CGACTTTGCT CTGTTATCTC 
TATTCAGACC TGTATAAACA TACCAATTCC AAAGAAACTC GTCGCATCTT CCTTGAGTTT 
CATCAGTTCT TTCTAGATCG ATCAGCACAC CTGAAAGTTT CTGTTCCTGA TGAAATGTCT 
GCAGATCTAG AAAAGAGAAG ACCTGAGCTC ATTCCTGAGG ATCTGCATCG CCACTATATC 
CAAACTATGC AAGAAAGAGT CCATCCAGAA GTTCAAAGGC ACTTAGAAGA TTTTCGGCAG 
AAACGTAGTA TGGGACTGAC CTTGGCTGAA AGCGAGCTGA CTAAACTTGA TGCAGAGCGA 
GACAAGGACC GATTGACTTT GGAGAAGGAG CGGACATGTG CAGAACAGAT TGTTGCCAAA 
ATTGAAGAAG TATTGATGAC TGCTCAGGCT GTAGAGGAAG ATAAGAGCTC CACCATGCAG 
TATGTTATTC TCATGTATAT GAAGCATTTG GGAGTAAAAG TGAAAGAGCC TCGAAATTTG 
GAGCACAAAC GGGGTCGGAT TGGATTTCTT CCCAAAATCA AGCAAAGTAT GAAGAAAGAT 
AAAGAAGGGG AAGAAAAAGG GAAGCGAAGA GGATTCCCCA GCATCCTGGG ACCCCCACGG 
AGACCAAGCC GTCATGACAA CAGTGCAATT GGCAGAGCCA TGGAACTACA GAAGGCGCGC 
CACCCTAAGC ACTTATCCAC ACCCTCATCT GTGAGTCCTG AACCTCAGGA CTCTGCCAAG 
TTGCGCCAGA GTGGGTTAGC AAATGAAGGA ACAGACGCTG GATACCTGCC TGCCAATTCC 
ATGTCTTCTG TAGCTTCAGG GGCCTCTTTT TCCCAGGAAG GAGGGAAAGA GAATGATACA 
GGATCAAAGC AAGTTGGAGA AACATCAGCA CCTGGAGACA CCTTAGATGG CACACCTCGT 
ACTCTCAATA CTGTCTTTGA TTTCCCACCA CCTCCATTAG ACCAAGTGCA GGAGGAGGAA 
TGTGAAGTAG AAAGGGTGAC TGAACATGGG ACACCAAAGC CCTTTCGAAA GTTTGACAGT 
GTAGCTTTTG GAGAAAGTCA AAGTGAGGAT GAACAATTTG AAAATGACTT AGAGACAGAT 
CCACCCAACT GGCAGCAGCT TGTTAGTCGA GAAGTGTTAC TGGGACTAAA ACCTTGTGAA 
ATCAAAAGAC AGGAAGTGAT TAATGAATTG TTCTACACTG AAAGAGCTCA TGTTCGAACA 
CTGAAGGTTC TTGATCAAGT GTTCTATCAG CGAGTATCCA GAGAAGGAAT TCTGTCACCC 
TCAGAGCTAC GGAAAATTTT TTCAAACTTG GAAGATATTC TTCAACTTCA TATTGGATTG 
AATGAACAAA TGAAGGCTGT TCGAAAGAGA AATGAGACCT CTGTTATCGA TCAGATTGGG 
GAAGATTTGC TGACATGGTT CAGCGGACCA GGAGAGGAGA AATTGAAACA TGCTGCTGCT 
ACCTTTTGCA GTAACCAACC TTTCGCCCTG GAAATGATCA AATCTCGTCA GAAAAAGGAT 
TCTCGATTTC AGACTTTTGT GCAAGATGCT GAAAGTAATC CACTGTGTCG TCGTCTTCAA 
CTGAAGGATA TTATTCCCAC TCAAATGCAA AGGCTTACTA AGTACCCACT TCTGTTGGAT 
AATATTGCCA AATACACAGA ATGGCCAACA GAAAGGGAGA AGGTGAAGAA AGCTGCAGAT 
CACTGTCGTC AGATCTTAAA TTATGTAAAT CAGGCTGTCA AGGAGGCAGA AAACAAGCAG 
CGCCTAGAAG ATTATCAGCG TCGCCTTGAT ACCTCCAGCC TGAAGTTGTC AGAGTACCCA 
AATGTTGAAG AGCTCAGGAA TTTGGATTTA ACAAAAAGGA AGATGATTCA TGAAGGGCCA 
TTGGTTTGGA AGGTGAATAG AGATAAAACT ATTGATTTAT ACACGTTGCT GCTGGAAGAC 
ATTCTTGTAT TGTTACAAAA GCAGGATGAT AGACTGGTTT TAAGGTGTCA TAGTAAGATT 
CTGGCATCTA CAGCTGATAG CAAACACACG TTTAGCCCTG TCATTAAGTT GAGTACAGTG 
TTGGTTCGAC AAGTGGCAAC AGATAACAAA GCTTTATTCG TCATTTCCAT GTCAGACAAT 
GGCGCTCAGA TTTATGAACT GGTGGCACAG ACAGTTTCTG AAAAGACTGT CTGGCAGGAC 
CTAATCTGTC GGATGGCTGC ATCAGTGAAG GAGCAATCCA CAAAGCCAAT TCCATTACCA 
CAGTCAACAC CTGGCGAAGG AGATAATGAT GAAGAAGATC CTTCAAAATT AAAAGAGGAG 
CAGCATGGCA TTTCAGTCAC TGGTTTGCAG AGTCCAGACA GAGATTTGGG ATTAGAATCT 
ACCTTAATAT CGTCAAAACC TCAGTCTCAT TCACTGAGTA CCTCTGGGAA ATCAGAGGTA 
CGTGATCTGT TTGTGGCTGA GAGACAGTTT GCAAAGGAAC AACATACAGA TGGGACACTA 
AAGGAAGTTG GAGAAGATTA TCAAATCGCA ATCCCAGATT CACACCTGCC TGTCTCAGAA 
GAACGGTGGG CATTGGATGC ACTAAGAAAT TTGGGTTTGT TGAAGCAGTT GCTGGTGCAA 
CAGCTAGGTT TGACTGAGAA GAGCGTTCAG GAAGACTGGC AACATTTCCC AAGATACAGA 
ACAGCCTCTC AGGGGCCGCA GACAGACAGT GTCATCCAGA ACTCTGAAAA TATTAAGGCC 
TATCATTCTG GTGAAGGACA TATGCCCTTT AGAACTGGAA CTGGTGACAT TGCAACTTGT 
TACAGTCCAC GGACTTCAAC TGAATCTTTT GCTCCACGGG ATTCAGTGGG ACTGGCACCC 
CAGGATAGCC AGGCAAGTAA CATTTTAGTA ATGGACCACA TGATTATGAC CCCAGAGATG 
CCTACCATGG AGCCAGAAGG GGGTCTTGAT GACAGTGGAG AGCACTTTTT TGATGCCCGT 
GAAGCACATA GTGATGAGAA TCCATCAGAA GGTGATGGAG CAGTTAACAA GGAAGAGAAG 
GATGTTAATT TACGCATCTC AGGAAACTAT TTGATCCTTG ATGGCTATGA CCCAGTGCAG 
GAGAGTTCCA CAGATGAGGA GGTTGCTTCC TCACTTACCC TGCAGCCCAT GACAGGCATC 
CCTGCTGTGG AATCCACCCA CCAGCAGCAA CATTCTCCTC AGAATACTCA CTCCGATGGG 
GCAATTTCAC CATTCACCCC CGAATTTCTG GTCCAGCAGC GCTGGGGAGC TATGGAGTAT 
TCCTGTTTTG AGATCCAGAG TCCCTCCTCT TGTGCAGATT CACAGAGCCA GATCATGGAG 
TACATTCATA AGATAGAGGC TGACCTTGAA CACTTAAAGA AGGTGGAGGA AAGTTACACC 
ATTCTTTGCC AAAGGCTGGC TGGATCAGCC CTCACAGACA AGCACTCAGA TAAAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_017017421.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016872910.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu45709
Clone ID Related Accession (Same CDS sequence)	XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
Accession Version	XM_017017421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016872910.1
CDS	861..5186
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_017017421.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017017421.1

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF12 ( XM_011542720.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541022.1 Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

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CloneID	OHu45709
Clone ID Related Accession (Same CDS sequence)	XM_017017421.1 , NM_001301084.1 , XM_011542720.2 , NM_001301084.2
Accession Version	XM_011542720.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011542720.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541022.1
CDS	586..4911
Translation	MRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVIGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKKHIPQLQEQLSKATGSAQDGAVVTPSRPLGDTLTVSEAETDPGDVLGRTDCSSGDASRPSSDNADSPKSGPKERIYLEENPEKSETIQDTDTQSLVGSPSTRIAPHIIGAEDDDFGTEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKHTNSKETRRIFLEFHQFFLDRSAHLKVSVPDEMSADLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDAERDKDRLTLEKERTCAEQIVAKIEEVLMTAQAVEEDKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDKEGEEKGKRRGFPSILGPPRRPSRHDNSAIGRAMELQKARHPKHLSTPSSVSPEPQDSAKLRQSGLANEGTDAGYLPANSMSSVASGASFSQEGGKENDTGSKQVGETSAPGDTLDGTPRTLNTVFDFPPPPLDQVQEEECEVERVTEHGTPKPFRKFDSVAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAVRKRNETSVIDQIGEDLLTWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSSLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLSTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQSTPGEGDNDEEDPSKLKEEQHGISVTGLQSPDRDLGLESTLISSKPQSHSLSTSGKSEVRDLFVAERQFAKEQHTDGTLKEVGEDYQIAIPDSHLPVSEERWALDALRNLGLLKQLLVQQLGLTEKSVQEDWQHFPRYRTASQGPQTDSVIQNSENIKAYHSGEGHMPFRTGTGDIATCYSPRTSTESFAPRDSVGLAPQDSQASNILVMDHMIMTPEMPTMEPEGGLDDSGEHFFDAREAHSDENPSEGDGAVNKEEKDVNLRISGNYLILDGYDPVQESSTDEEVASSLTLQPMTGIPAVESTHQQQHSPQNTHSDGAISPFTPEFLVQQRWGAMEYSCFEIQSPSSCADSQSQIMEYIHKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	XM_011542720.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011542720.2

ORF Insert Sequence:

ATGCGGGCTG GAGTACAGAC AGGTGATCGA ATCATCAAGG TGAATGGAAC TCTGGTGACT 
CATTCAAATC ATCTGGAGGT GGTGAAGCTA ATCAAATCTG GTTCCTATGT AGCTCTCACT 
GTTCAGGGAC GCCCACCTGG GTCGCCCCAG ATTCCACTTG CCGACTCTGA AGTAGAGCCG 
TCAGTCATTG GACATATGTC TCCCATCATG ACATCTCCTC ATTCACCTGG AGCATCTGGG 
AATATGGAGA GAATCACTAG TCCTGTGCTC ATGGGGGAGG AAAACAATGT GGTTCATAAC 
CAGAAAGTAG AAATTCTGAG AAAAATGTTA CAGAAAGAAC AGGAACGGCT ACAGTTATTG 
CAGGAAGATT ACAACCGAAC ACCTGCCCAA AGATTGCTAA AAGAGATCCA AGAGGCCAAG 
AAACACATTC CTCAGCTGCA AGAGCAGTTA TCCAAAGCCA CAGGCTCTGC TCAGGATGGA 
GCTGTAGTTA CACCCTCCAG ACCTTTAGGG GACACCCTAA CAGTCAGTGA GGCAGAAACA 
GATCCTGGAG ATGTACTGGG CAGGACTGAC TGTAGCAGTG GAGATGCTTC TCGGCCCAGT 
AGTGACAATG CAGATAGTCC CAAGAGTGGC CCAAAAGAGA GAATTTATCT AGAGGAAAAC 
CCAGAGAAAA GTGAAACAAT TCAGGACACT GACACTCAAT CACTTGTCGG AAGTCCCTCA 
ACCCGTATAG CACCTCATAT TATTGGAGCA GAAGATGATG ATTTTGGTAC TGAACATGAA 
CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TACTAAAATC TCGCCCGGCT 
CATTTGGCTG TTTTCTTACA CCATGTAGTT TCACAATTTG ACCCTGCGAC TTTGCTCTGT 
TATCTCTATT CAGACCTGTA TAAACATACC AATTCCAAAG AAACTCGTCG CATCTTCCTT 
GAGTTTCATC AGTTCTTTCT AGATCGATCA GCACACCTGA AAGTTTCTGT TCCTGATGAA 
ATGTCTGCAG ATCTAGAAAA GAGAAGACCT GAGCTCATTC CTGAGGATCT GCATCGCCAC 
TATATCCAAA CTATGCAAGA AAGAGTCCAT CCAGAAGTTC AAAGGCACTT AGAAGATTTT 
CGGCAGAAAC GTAGTATGGG ACTGACCTTG GCTGAAAGCG AGCTGACTAA ACTTGATGCA 
GAGCGAGACA AGGACCGATT GACTTTGGAG AAGGAGCGGA CATGTGCAGA ACAGATTGTT 
GCCAAAATTG AAGAAGTATT GATGACTGCT CAGGCTGTAG AGGAAGATAA GAGCTCCACC 
ATGCAGTATG TTATTCTCAT GTATATGAAG CATTTGGGAG TAAAAGTGAA AGAGCCTCGA 
AATTTGGAGC ACAAACGGGG TCGGATTGGA TTTCTTCCCA AAATCAAGCA AAGTATGAAG 
AAAGATAAAG AAGGGGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT CCTGGGACCC 
CCACGGAGAC CAAGCCGTCA TGACAACAGT GCAATTGGCA GAGCCATGGA ACTACAGAAG 
GCGCGCCACC CTAAGCACTT ATCCACACCC TCATCTGTGA GTCCTGAACC TCAGGACTCT 
GCCAAGTTGC GCCAGAGTGG GTTAGCAAAT GAAGGAACAG ACGCTGGATA CCTGCCTGCC 
AATTCCATGT CTTCTGTAGC TTCAGGGGCC TCTTTTTCCC AGGAAGGAGG GAAAGAGAAT 
GATACAGGAT CAAAGCAAGT TGGAGAAACA TCAGCACCTG GAGACACCTT AGATGGCACA 
CCTCGTACTC TCAATACTGT CTTTGATTTC CCACCACCTC CATTAGACCA AGTGCAGGAG 
GAGGAATGTG AAGTAGAAAG GGTGACTGAA CATGGGACAC CAAAGCCCTT TCGAAAGTTT 
GACAGTGTAG CTTTTGGAGA AAGTCAAAGT GAGGATGAAC AATTTGAAAA TGACTTAGAG 
ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGTCGAGAAG TGTTACTGGG ACTAAAACCT 
TGTGAAATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAAAG AGCTCATGTT 
CGAACACTGA AGGTTCTTGA TCAAGTGTTC TATCAGCGAG TATCCAGAGA AGGAATTCTG 
TCACCCTCAG AGCTACGGAA AATTTTTTCA AACTTGGAAG ATATTCTTCA ACTTCATATT 
GGATTGAATG AACAAATGAA GGCTGTTCGA AAGAGAAATG AGACCTCTGT TATCGATCAG 
ATTGGGGAAG ATTTGCTGAC ATGGTTCAGC GGACCAGGAG AGGAGAAATT GAAACATGCT 
GCTGCTACCT TTTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAATC TCGTCAGAAA 
AAGGATTCTC GATTTCAGAC TTTTGTGCAA GATGCTGAAA GTAATCCACT GTGTCGTCGT 
CTTCAACTGA AGGATATTAT TCCCACTCAA ATGCAAAGGC TTACTAAGTA CCCACTTCTG 
TTGGATAATA TTGCCAAATA CACAGAATGG CCAACAGAAA GGGAGAAGGT GAAGAAAGCT 
GCAGATCACT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAAGGA GGCAGAAAAC 
AAGCAGCGCC TAGAAGATTA TCAGCGTCGC CTTGATACCT CCAGCCTGAA GTTGTCAGAG 
TACCCAAATG TTGAAGAGCT CAGGAATTTG GATTTAACAA AAAGGAAGAT GATTCATGAA 
GGGCCATTGG TTTGGAAGGT GAATAGAGAT AAAACTATTG ATTTATACAC GTTGCTGCTG 
GAAGACATTC TTGTATTGTT ACAAAAGCAG GATGATAGAC TGGTTTTAAG GTGTCATAGT 
AAGATTCTGG CATCTACAGC TGATAGCAAA CACACGTTTA GCCCTGTCAT TAAGTTGAGT 
ACAGTGTTGG TTCGACAAGT GGCAACAGAT AACAAAGCTT TATTCGTCAT TTCCATGTCA 
GACAATGGCG CTCAGATTTA TGAACTGGTG GCACAGACAG TTTCTGAAAA GACTGTCTGG 
CAGGACCTAA TCTGTCGGAT GGCTGCATCA GTGAAGGAGC AATCCACAAA GCCAATTCCA 
TTACCACAGT CAACACCTGG CGAAGGAGAT AATGATGAAG AAGATCCTTC AAAATTAAAA 
GAGGAGCAGC ATGGCATTTC AGTCACTGGT TTGCAGAGTC CAGACAGAGA TTTGGGATTA 
GAATCTACCT TAATATCGTC AAAACCTCAG TCTCATTCAC TGAGTACCTC TGGGAAATCA 
GAGGTACGTG ATCTGTTTGT GGCTGAGAGA CAGTTTGCAA AGGAACAACA TACAGATGGG 
ACACTAAAGG AAGTTGGAGA AGATTATCAA ATCGCAATCC CAGATTCACA CCTGCCTGTC 
TCAGAAGAAC GGTGGGCATT GGATGCACTA AGAAATTTGG GTTTGTTGAA GCAGTTGCTG 
GTGCAACAGC TAGGTTTGAC TGAGAAGAGC GTTCAGGAAG ACTGGCAACA TTTCCCAAGA 
TACAGAACAG CCTCTCAGGG GCCGCAGACA GACAGTGTCA TCCAGAACTC TGAAAATATT 
AAGGCCTATC ATTCTGGTGA AGGACATATG CCCTTTAGAA CTGGAACTGG TGACATTGCA 
ACTTGTTACA GTCCACGGAC TTCAACTGAA TCTTTTGCTC CACGGGATTC AGTGGGACTG 
GCACCCCAGG ATAGCCAGGC AAGTAACATT TTAGTAATGG ACCACATGAT TATGACCCCA 
GAGATGCCTA CCATGGAGCC AGAAGGGGGT CTTGATGACA GTGGAGAGCA CTTTTTTGAT 
GCCCGTGAAG CACATAGTGA TGAGAATCCA TCAGAAGGTG ATGGAGCAGT TAACAAGGAA 
GAGAAGGATG TTAATTTACG CATCTCAGGA AACTATTTGA TCCTTGATGG CTATGACCCA 
GTGCAGGAGA GTTCCACAGA TGAGGAGGTT GCTTCCTCAC TTACCCTGCA GCCCATGACA 
GGCATCCCTG CTGTGGAATC CACCCACCAG CAGCAACATT CTCCTCAGAA TACTCACTCC 
GATGGGGCAA TTTCACCATT CACCCCCGAA TTTCTGGTCC AGCAGCGCTG GGGAGCTATG 
GAGTATTCCT GTTTTGAGAT CCAGAGTCCC TCCTCTTGTG CAGATTCACA GAGCCAGATC 
ATGGAGTACA TTCATAAGAT AGAGGCTGAC CTTGAACACT TAAAGAAGGT GGAGGAAAGT 
TACACCATTC TTTGCCAAAG GCTGGCTGGA TCAGCCCTCA CAGACAAGCA CTCAGATAAA 
AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ARHGEF12
Entrez Gene ID	23365
Full Name	Rho guanine nucleotide exchange factor 12
Synonyms	LARG,PRO2792
General protein information	Preferred Names Rho guanine nucleotide exchange factor 12 Names rho guanine nucleotide exchange factor 12 Rho guanine nucleotide exchange factor (GEF) 12 leukemia-associated RhoGEF leukemia-associated rho guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q23.3
Summary	Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli working through G protein-coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. This protein has been observed to form a myeloid/lymphoid fusion partner in acute myeloid leukemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014].
Disorder MIM:	604763

Canis lupus familiaris (dog)	ARHGEF12	XP_005619719.1
	ARHGEF12	XP_002799913.1
Gallus gallus (chicken)	ARHGEF12	XP_417890.4
Danio rerio (zebrafish)	arhgef12	XP_005172066.1
Pan troglodytes (chimpanzee)	ARHGEF12	XP_001166830.1
Xenopus tropicalis (tropical clawed frog)	arhgef12	NP_001072496.1
Homo sapiens (human)	ARHGEF12	NP_056128.1
Bos taurus (cattle)	ARHGEF12	NP_001116505.1
Mus musculus (house mouse)	Arhgef12	NP_081420.2
Rattus norvegicus (Norway rat)	Arhgef12	NP_001013264.1
Danio rerio (zebrafish)	LOC562455	XP_003200092.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ARHGEF12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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