arhgef12b cDNA ORF clone, Danio rerio(zebrafish)

The following arhgef12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144618 XM_021471689.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
ODa144619 XM_021471690.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $258.30
$369.00
ODa144620 XM_021471691.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
ODa59288 XM_017356361.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa59289 XM_017356362.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa46069 XM_009301885.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46070 XM_009301886.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46071 XM_009301887.3
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa46069 XM_009301885.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46070 XM_009301886.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa46071 XM_009301887.2 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa59289 XM_017356362.1 Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144618
    Clone ID Related Accession (Same CDS sequence) XM_021471689.1
    Accession Version XM_021471689.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGGTTAGTGT GACACTGGCT
    TGGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327364.1
    CDS1353..2381
    Translation

    Target ORF information:

    RefSeq Version XM_021471689.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471689.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGGTTAGTGT GACACTGGCT
    TGGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144619
    Clone ID Related Accession (Same CDS sequence) XM_021471690.1
    Accession Version XM_021471690.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGACAGGAA GTATTCTCAG CAAGGACCAT 
    CCCCCAGATA AAAAGGCCAA AAGCGACAAG GCGTCAGTCC CCCTCTCGCA TGAGTTTGAC
    CCCACAGGGC TTGTGCAGCG TTGTGTTATC ATCCAGAAAG ATGAGAATGG CTTTGGGCTG
    ACAGTGAGCG GCGATAACCC TGTGTTTGTA CAGCTTGTAA AAGAGGATGG AGCTGCCATG
    CGAGCTGGTG TTCAGACTGG TGACCGGATC ATAAAGGTCA ATGGGACGTT AGTTACACAT
    TCAAATCATG TAGAAGTTGT AAAGCTAATA AAATCGGGCT CGTATGTGGC CCTGACGGTG
    CTGGGGAGAC CGCCTGGCTT GCCTCAGATT CCCCTGTCAG ACGGCGAGGG GGAAGAAGGG
    TCTCCGTTCT CGCTCAGTTC TCCTCACTCC CCAGGGCCCA TCGGACCAGA GCGCTCCTGT
    TCCTCCCCCA GCCCCTCAGA GCGAAAAGCA GCCTCTCTGC CTGTATGGGG CATAACAGAG
    GACTACAGAA ACCCTACATC TAAACTACTG AAGGAAATCC AGGACATTCC ACAGAACCAG
    AACCAAGAGC AGCTCAGCAA ACCCACTGGA CAGGATGAAT CCATATCTCC GAGACCAGGT
    GAAGGAGAGC TGGAGGATTT TAGTGGAGAT GTGGATCGAC CCCCTTCCTG GCAGGGTGAT
    GCTGGGACGG AGCCATTATG GACCAACAAT AACATGACTC CAGTTACTCC TTGTCTTTTC
    CCAGAGAGCC CATGCCAAAG AGAGACCCCC TGCCACAGCC CAAAGACCAC ACCTCGAGAC
    AGCCTCAACT CCTGTCCCTC GCCGGATGCA GAAGACACAC CTGACCTAGA TTCTCAGTCA
    AGTGTAGGGA GCCCTTGCTC TCGTCCTGTA GCACATATCA TCGGTGCTGA GGATGAAGAC
    TTTGGCAAAG AGCAGGAACA GGTTAGTGTG ACACTGGCTT GGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327365.1
    CDS1353..2360
    Translation

    Target ORF information:

    RefSeq Version XM_021471690.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471690.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGACAGGAA GTATTCTCAG CAAGGACCAT 
    CCCCCAGATA AAAAGGCCAA AAGCGACAAG GCGTCAGTCC CCCTCTCGCA TGAGTTTGAC
    CCCACAGGGC TTGTGCAGCG TTGTGTTATC ATCCAGAAAG ATGAGAATGG CTTTGGGCTG
    ACAGTGAGCG GCGATAACCC TGTGTTTGTA CAGCTTGTAA AAGAGGATGG AGCTGCCATG
    CGAGCTGGTG TTCAGACTGG TGACCGGATC ATAAAGGTCA ATGGGACGTT AGTTACACAT
    TCAAATCATG TAGAAGTTGT AAAGCTAATA AAATCGGGCT CGTATGTGGC CCTGACGGTG
    CTGGGGAGAC CGCCTGGCTT GCCTCAGATT CCCCTGTCAG ACGGCGAGGG GGAAGAAGGG
    TCTCCGTTCT CGCTCAGTTC TCCTCACTCC CCAGGGCCCA TCGGACCAGA GCGCTCCTGT
    TCCTCCCCCA GCCCCTCAGA GCGAAAAGCA GCCTCTCTGC CTGTATGGGG CATAACAGAG
    GACTACAGAA ACCCTACATC TAAACTACTG AAGGAAATCC AGGACATTCC ACAGAACCAG
    AACCAAGAGC AGCTCAGCAA ACCCACTGGA CAGGATGAAT CCATATCTCC GAGACCAGGT
    GAAGGAGAGC TGGAGGATTT TAGTGGAGAT GTGGATCGAC CCCCTTCCTG GCAGGGTGAT
    GCTGGGACGG AGCCATTATG GACCAACAAT AACATGACTC CAGTTACTCC TTGTCTTTTC
    CCAGAGAGCC CATGCCAAAG AGAGACCCCC TGCCACAGCC CAAAGACCAC ACCTCGAGAC
    AGCCTCAACT CCTGTCCCTC GCCGGATGCA GAAGACACAC CTGACCTAGA TTCTCAGTCA
    AGTGTAGGGA GCCCTTGCTC TCGTCCTGTA GCACATATCA TCGGTGCTGA GGATGAAGAC
    TTTGGCAAAG AGCAGGAACA GGTTAGTGTG ACACTGGCTT GGTTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144620
    Clone ID Related Accession (Same CDS sequence) XM_021471691.1
    Accession Version XM_021471691.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 924bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394618.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG ATGAATCCAT ATCTCCGAGA CCAGGTGAAG GAGAGCTGGA GGATTTTAGT
    GGAGATGTGG ATCGACCCCC TTCCTGGCAG GGTGATGCTG GGACGGAGCC ATTATGGACC
    AACAATAACA TGACTCCAGT TACTCCTTGT CTTTTCCCAG AGAGCCCATG CCAAAGAGAG
    ACCCCCTGCC ACAGCCCAAA GACCACACCT CGAGACAGCC TCAACTCCTG TCCCTCGCCG
    GATGCAGAAG ACACACCTGA CCTAGATTCT CAGTCAAGTG TAGGGAGCCC TTGCTCTCGT
    CCTGTAGCAC ATATCATCGG TGCTGAGGAT GAAGACTTTG GCAAAGAGCA GGAACAGGTT
    AGTGTGACAC TGGCTTGGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327366.1
    CDS1353..2276
    Translation

    Target ORF information:

    RefSeq Version XM_021471691.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG ATGAATCCAT ATCTCCGAGA CCAGGTGAAG GAGAGCTGGA GGATTTTAGT
    GGAGATGTGG ATCGACCCCC TTCCTGGCAG GGTGATGCTG GGACGGAGCC ATTATGGACC
    AACAATAACA TGACTCCAGT TACTCCTTGT CTTTTCCCAG AGAGCCCATG CCAAAGAGAG
    ACCCCCTGCC ACAGCCCAAA GACCACACCT CGAGACAGCC TCAACTCCTG TCCCTCGCCG
    GATGCAGAAG ACACACCTGA CCTAGATTCT CAGTCAAGTG TAGGGAGCCC TTGCTCTCGT
    CCTGTAGCAC ATATCATCGG TGCTGAGGAT GAAGACTTTG GCAAAGAGCA GGAACAGGTT
    AGTGTGACAC TGGCTTGGTT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59288
    Clone ID Related Accession (Same CDS sequence) XM_017356361.1
    Accession Version XM_017356361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG TAGAAAAAGA CGACACTGAG
    GTCATCCCAA CCCCACTACC CAGCACCAAC CCCTTTGAGA CCAAATCTGA TGAGGAGGCT
    GAAGAAGAGG AGGAAAACTC TCTACAGGAC CAGGCTGAAG ACGATGAAGC GTTTGTTGTG
    GCAGATCTGA GCGATCGACT GACGTTCCTG AAACAGCGCT CCAGACTCGG CATCGCCATC
    GATGAGGACG AGGCCGAAGT CTTCGAGCTG CAGCCGCTCA TAGCTGATGA AGCTCTCAGG
    ACATTGGCGA CATTGAGGCA ACTCCTGATC AACCACTTTT CCGGTCAGGA GGACACAGAC
    AGAGAGAACA AGAGTGAAGA TCAACAGGAG CATATAGAGA GGACGTGCCC TCAGCAGGGC
    TCAGACGACA GCGCTGCCAT CCAGTCTCCG CTTGAGAACG GCTCAGACAG AGGAGAGAAT
    CCACAGGAAC TACCATCCGG GGACACCGGC TTCTTTGAGA CGTCTGAAGA TTGTGTGTCT
    GCAGGCAGTT ATATGGTGCT GGAGGTGTTT GCGGGCTCAG GAGAGAGCAG CACAGATGAC
    GATGCGCAGG GCTGCAGTGT GGAGCCTGTG GCTGGTGGAG ATTCAGGCAT CGACTTGAAG
    AAGCTCCTGT CTTCATCTTC TCAAAGCACT GGAGGACCCA ACATCAACAG ACAGATCATG
    ACACACTTAC GTCTACTACA GGGCAACCTG CAGCAGCTGA AGGAGACCGA AATCAAGTAT
    AACCAGCTAC GCCAAAGGCT ATCAGAGGAA ACAGCTACTG ACACAGAGGA AAACAAAGGT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211850.1
    CDS1449..5714
    Translation

    Target ORF information:

    RefSeq Version XM_017356361.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG TAGAAAAAGA CGACACTGAG
    GTCATCCCAA CCCCACTACC CAGCACCAAC CCCTTTGAGA CCAAATCTGA TGAGGAGGCT
    GAAGAAGAGG AGGAAAACTC TCTACAGGAC CAGGCTGAAG ACGATGAAGC GTTTGTTGTG
    GCAGATCTGA GCGATCGACT GACGTTCCTG AAACAGCGCT CCAGACTCGG CATCGCCATC
    GATGAGGACG AGGCCGAAGT CTTCGAGCTG CAGCCGCTCA TAGCTGATGA AGCTCTCAGG
    ACATTGGCGA CATTGAGGCA ACTCCTGATC AACCACTTTT CCGGTCAGGA GGACACAGAC
    AGAGAGAACA AGAGTGAAGA TCAACAGGAG CATATAGAGA GGACGTGCCC TCAGCAGGGC
    TCAGACGACA GCGCTGCCAT CCAGTCTCCG CTTGAGAACG GCTCAGACAG AGGAGAGAAT
    CCACAGGAAC TACCATCCGG GGACACCGGC TTCTTTGAGA CGTCTGAAGA TTGTGTGTCT
    GCAGGCAGTT ATATGGTGCT GGAGGTGTTT GCGGGCTCAG GAGAGAGCAG CACAGATGAC
    GATGCGCAGG GCTGCAGTGT GGAGCCTGTG GCTGGTGGAG ATTCAGGCAT CGACTTGAAG
    AAGCTCCTGT CTTCATCTTC TCAAAGCACT GGAGGACCCA ACATCAACAG ACAGATCATG
    ACACACTTAC GTCTACTACA GGGCAACCTG CAGCAGCTGA AGGAGACCGA AATCAAGTAT
    AACCAGCTAC GCCAAAGGCT ATCAGAGGAA ACAGCTACTG ACACAGAGGA AAACAAAGGT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59289
    Clone ID Related Accession (Same CDS sequence) XM_017356362.2 , XM_017356362.1
    Accession Version XM_017356362.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356362.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG ATGAATCCAT ATCTCCGAGA CCAGGTGAAG GAGAGCTGGA GGATTTTAGT
    GGAGATGTGG ATCGACCCCC TTCCTGGCAG GGTGATGCTG GGACGGAGCC ATTATGGACC
    AACAATAACA TGACTCCAGT TACTCCTTGT CTTTTCCCAG AGAGCCCATG CCAAAGAGAG
    ACCCCCTGCC ACAGCCCAAA GACCACACCT CGAGACAGCC TCAACTCCTG TCCCTCGCCG
    GATGCAGAAG ACACACCTGA CCTAGATTCT CAGTCAAGTG TAGGGAGCCC TTGCTCTCGT
    CCTGTAGCAC ATATCATCGG TGCTGAGGAT GAAGACTTTG GCAAAGAGCA GGAACAGATG
    AATGGCCAGT GTAGCTGTTT TCAGAGCATA GACCTCCTGA AATCTCGACC TGCTCACCTG
    GCAGCTTTCA TCCATCACGT AGTCTCTCAG TTTGACCCTG CACCGCTGCT CTGCTACCTC
    TACGCTGATC TTTACAAACA ATCCAGCACC AAAGAGACAC GACGAATCCT CATGGATACC
    TTCTTCATAG ACAAAACAGC TAATCTGAAA GTAACAGTAC CTGAATCCAT CATCGCTGAC
    CTGGAGAGGA CCGCACACTC AACCAATGCA GAGCGGAAGA GGCCGGAGCA AATGTCTGAA
    GATGTACACC GACAATATGT GCAACAACTG CAGGACACTC TTCTAACTGA CGTTCAGAAG
    AACCTAGAAG ACTTTAGGCA CAAGCGTAGT ATGGGTCTGA CATTGGCTGA AGCTGAACTG
    GCCCGTCTGG ATACAGAGAG AATGAGAGAT CGTGTGGCTT TGGAAAAAGA GCGATCCTGT
    GCAGAGCACA TCCTTTCTAA GATAGAAGAA GTCCTATTGA CGTCACAAGC CACAGAGGAA
    GATAAGTGTA GCACCATGCA ATTTGTGATA CTATCGTATA TGAAACAGCT CGGTGTGAAA
    GTGAAGGAGT TGCGAGGGCT GGACCACAAA CCCAAGTTAA TGAATCCATT CCACAAAATA
    AAGAAAAGTA TGAAGCCTGA GAAGGATGGA AGTGAGGAGA AAGCGAAGAA AACACGATTT
    CCTAATGTCT TTCCTCAGAG ACGACCAAGC AAGATTGACG CTTTATCACA GCCGAAGCAG
    CGTACGCCAA AGCCTCAGCT GCCTCTGGGA ACTGGAGAGC AGGCAGACGG GTCGTCTCTT
    CCTGTACGGA GCAGTCAGTC CAGCGAGGGC TCAGATGGCA CCAATGCCCT GATCACATCA
    CCGCCTTACA GCGCTCCAGC AGGACAGGGC ACAGACGCCA TCAGCCGAGA GTCTGAGACT
    GGGGGACTTC CCTCTTCTGC CCTGCCCAAA TTGGGAGAGG GTACTGTCTC AGGGGATTTG
    CAGGACTCCT CATCTACAAA CACTCACTTT GACTTCAGCA ATTGCATGCT GGAGCATTTA
    CAGGAGGAAG AAGCTGAGAC TGAAAAGACG CAGGACTCTG GCACCCCCAG GCCTGACAGG
    AGCAGGATGG ATCCGCTGGG CTCTGGAGAG GTGCAGAGTG AAGATGATCA GTGTGTTGAA
    GCCGAACTCG AGCCTCCAAA CTGGCAGACA CTAGTGAGCA GAGACGTCCT GTCCACACTC
    ACGCCTCAGG AAATTAAGAG ACAAGAAGTC ATCAATGAGC TGTTCTACAC GGAGCGAGCA
    CACGTTCGAA TGATGAAGGT TCTGGAGAAT TTATTTTACC AGCCGCTGAT CAGAGACGCC
    ATCCTTCCTC CTGCAGACAT CAAAAACATC TTCAGCAACC TGGAGGAGAT CGTTCAGCTG
    CACTTGTCTT TAACCGAACA AATGGCCGCC GTACGGAAGA AGAACGAGAC CGCTCTCATT
    GACTCAATAG GAGATGATCT GCTTTCCTGG TTCAGTGAAG AAGAGGAGGA TAAGATCAAG
    AGAGCGGTGG GCACCTACTG CAGTAACCAG CCCTTCGCCC TGGAGCTCAT CAAGAGCAAA
    CAGAAGAAAG ACCAGCGCTT CAATGCCTTC ATACAGGAGA CAGAGAGCAA CCGTCATTGT
    CGCAGGCTGC AGTTGAAAGA CATTATTCCT GTGGAGACGT TACGACTCAC AAAGTATCCT
    CTTCTGCTGG AGAACATCGC CAAATACACA GAGGACTCTG AGGAGAGAAG TAAAGTGAAA
    CGGGCTGGCG AATGCTGTAG AAAGATCCTC ACTCACGTCA ACCAGGAGGT GAAGGAAGCG
    GAAAACAAGC AGAGGCTGGA GGACTACCAG AGAAGACTGG ACCTGTCCTC ACTGAAGCAG
    AGCGAAAACC CCATGATCGC AGAGTTCAAG AGCTTGGACT TGACCAAGAG GAAGATGGTG
    CACGAGGGGC CGCTGTCCTG GAAGGTCAAC AAAGACAAAA CCATTGAGCT GTACACGTTG
    CTGCTGGAGG ATATATTGGT GCTACTTCAA AGGCAGGATG AGAAGCTGAT CTTAAAATGC
    CACAGTAAGA ATCTGGCGGG CACCGCTGAG ACCAAACACA TCTTCAGCCC CATCATCAAG
    CTCAGCACTG TGATGGTGCG CTCCGTTGCC ACCGACAACA GGTCGTTCTT TGTGCTGTCG
    ATGTCTGATA ATGGAGCCCA GATCTATGAG CTGATGGCCC AGACCGTATC AGAGCAAAAG
    ACGTGGCAGT GTCTGATCAC ACAAAGAGCA GACTGCATGA AGACCAAACT CCACAACATC
    ATTCCATTAC CTCAGACCGA TGGGGAACGT GAAGAGGTGG AGATGATCAA CTCTGGTGTT
    CAGAAGATAA GCAAAGACAC TGAACCAATT ACTTTAGCGA GCATCCAGTC TCCAGTAGAA
    AAAGACGACA CTGAGGTCAT CCCAACCCCA CTACCCAGCA CCAACCCCTT TGAGACCAAA
    TCTGATGAGG AGGCTGAAGA AGAGGAGGAA AACTCTCTAC AGGACCAGGC TGAAGACGAT
    GAAGCGTTTG TTGTGGCAGA TCTGAGCGAT CGACTGACGT TCCTGAAACA GCGCTCCAGA
    CTCGGCATCG CCATCGATGA GGACGAGGCC GAAGTCTTCG AGCTGCAGCC GCTCATAGCT
    GATGAAGCTC TCAGGACATT GGCGACATTG AGGCAACTCC TGATCAACCA CTTTTCCGGT
    CAGGAGGACA CAGACAGAGA GAACAAGAGT GAAGATCAAC AGGAGCATAT AGAGAGGACG
    TGCCCTCAGC AGGGCTCAGA CGACAGCGCT GCCATCCAGT CTCCGCTTGA GAACGGCTCA
    GACAGAGGAG AGAATCCACA GGAACTACCA TCCGGGGACA CCGGCTTCTT TGAGACGTCT
    GAAGATTGTG TGTCTGCAGG CAGTTATATG GTGCTGGAGG TGTTTGCGGG CTCAGGAGAG
    AGCAGCACAG ATGACGATGC GCAGGGCTGC AGTGTGGAGC CTGTGGCTGG TGGAGATTCA
    GGCATCGACT TGAAGAAGCT CCTGTCTTCA TCTTCTCAAA GCACTGGAGG ACCCAACATC
    AACAGACAGA TCATGACACA CTTACGTCTA CTACAGGGCA ACCTGCAGCA GCTGAAGGAG
    ACCGAAATCA AGTATAACCA GCTACGCCAA AGGCTATCAG AGGAAACAGC TACTGACACA
    GAGGAAAACA AAGATAAAAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211851.1
    CDS1368..5531
    Translation

    Target ORF information:

    RefSeq Version XM_017356362.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356362.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG ATGAATCCAT ATCTCCGAGA CCAGGTGAAG GAGAGCTGGA GGATTTTAGT
    GGAGATGTGG ATCGACCCCC TTCCTGGCAG GGTGATGCTG GGACGGAGCC ATTATGGACC
    AACAATAACA TGACTCCAGT TACTCCTTGT CTTTTCCCAG AGAGCCCATG CCAAAGAGAG
    ACCCCCTGCC ACAGCCCAAA GACCACACCT CGAGACAGCC TCAACTCCTG TCCCTCGCCG
    GATGCAGAAG ACACACCTGA CCTAGATTCT CAGTCAAGTG TAGGGAGCCC TTGCTCTCGT
    CCTGTAGCAC ATATCATCGG TGCTGAGGAT GAAGACTTTG GCAAAGAGCA GGAACAGATG
    AATGGCCAGT GTAGCTGTTT TCAGAGCATA GACCTCCTGA AATCTCGACC TGCTCACCTG
    GCAGCTTTCA TCCATCACGT AGTCTCTCAG TTTGACCCTG CACCGCTGCT CTGCTACCTC
    TACGCTGATC TTTACAAACA ATCCAGCACC AAAGAGACAC GACGAATCCT CATGGATACC
    TTCTTCATAG ACAAAACAGC TAATCTGAAA GTAACAGTAC CTGAATCCAT CATCGCTGAC
    CTGGAGAGGA CCGCACACTC AACCAATGCA GAGCGGAAGA GGCCGGAGCA AATGTCTGAA
    GATGTACACC GACAATATGT GCAACAACTG CAGGACACTC TTCTAACTGA CGTTCAGAAG
    AACCTAGAAG ACTTTAGGCA CAAGCGTAGT ATGGGTCTGA CATTGGCTGA AGCTGAACTG
    GCCCGTCTGG ATACAGAGAG AATGAGAGAT CGTGTGGCTT TGGAAAAAGA GCGATCCTGT
    GCAGAGCACA TCCTTTCTAA GATAGAAGAA GTCCTATTGA CGTCACAAGC CACAGAGGAA
    GATAAGTGTA GCACCATGCA ATTTGTGATA CTATCGTATA TGAAACAGCT CGGTGTGAAA
    GTGAAGGAGT TGCGAGGGCT GGACCACAAA CCCAAGTTAA TGAATCCATT CCACAAAATA
    AAGAAAAGTA TGAAGCCTGA GAAGGATGGA AGTGAGGAGA AAGCGAAGAA AACACGATTT
    CCTAATGTCT TTCCTCAGAG ACGACCAAGC AAGATTGACG CTTTATCACA GCCGAAGCAG
    CGTACGCCAA AGCCTCAGCT GCCTCTGGGA ACTGGAGAGC AGGCAGACGG GTCGTCTCTT
    CCTGTACGGA GCAGTCAGTC CAGCGAGGGC TCAGATGGCA CCAATGCCCT GATCACATCA
    CCGCCTTACA GCGCTCCAGC AGGACAGGGC ACAGACGCCA TCAGCCGAGA GTCTGAGACT
    GGGGGACTTC CCTCTTCTGC CCTGCCCAAA TTGGGAGAGG GTACTGTCTC AGGGGATTTG
    CAGGACTCCT CATCTACAAA CACTCACTTT GACTTCAGCA ATTGCATGCT GGAGCATTTA
    CAGGAGGAAG AAGCTGAGAC TGAAAAGACG CAGGACTCTG GCACCCCCAG GCCTGACAGG
    AGCAGGATGG ATCCGCTGGG CTCTGGAGAG GTGCAGAGTG AAGATGATCA GTGTGTTGAA
    GCCGAACTCG AGCCTCCAAA CTGGCAGACA CTAGTGAGCA GAGACGTCCT GTCCACACTC
    ACGCCTCAGG AAATTAAGAG ACAAGAAGTC ATCAATGAGC TGTTCTACAC GGAGCGAGCA
    CACGTTCGAA TGATGAAGGT TCTGGAGAAT TTATTTTACC AGCCGCTGAT CAGAGACGCC
    ATCCTTCCTC CTGCAGACAT CAAAAACATC TTCAGCAACC TGGAGGAGAT CGTTCAGCTG
    CACTTGTCTT TAACCGAACA AATGGCCGCC GTACGGAAGA AGAACGAGAC CGCTCTCATT
    GACTCAATAG GAGATGATCT GCTTTCCTGG TTCAGTGAAG AAGAGGAGGA TAAGATCAAG
    AGAGCGGTGG GCACCTACTG CAGTAACCAG CCCTTCGCCC TGGAGCTCAT CAAGAGCAAA
    CAGAAGAAAG ACCAGCGCTT CAATGCCTTC ATACAGGAGA CAGAGAGCAA CCGTCATTGT
    CGCAGGCTGC AGTTGAAAGA CATTATTCCT GTGGAGACGT TACGACTCAC AAAGTATCCT
    CTTCTGCTGG AGAACATCGC CAAATACACA GAGGACTCTG AGGAGAGAAG TAAAGTGAAA
    CGGGCTGGCG AATGCTGTAG AAAGATCCTC ACTCACGTCA ACCAGGAGGT GAAGGAAGCG
    GAAAACAAGC AGAGGCTGGA GGACTACCAG AGAAGACTGG ACCTGTCCTC ACTGAAGCAG
    AGCGAAAACC CCATGATCGC AGAGTTCAAG AGCTTGGACT TGACCAAGAG GAAGATGGTG
    CACGAGGGGC CGCTGTCCTG GAAGGTCAAC AAAGACAAAA CCATTGAGCT GTACACGTTG
    CTGCTGGAGG ATATATTGGT GCTACTTCAA AGGCAGGATG AGAAGCTGAT CTTAAAATGC
    CACAGTAAGA ATCTGGCGGG CACCGCTGAG ACCAAACACA TCTTCAGCCC CATCATCAAG
    CTCAGCACTG TGATGGTGCG CTCCGTTGCC ACCGACAACA GGTCGTTCTT TGTGCTGTCG
    ATGTCTGATA ATGGAGCCCA GATCTATGAG CTGATGGCCC AGACCGTATC AGAGCAAAAG
    ACGTGGCAGT GTCTGATCAC ACAAAGAGCA GACTGCATGA AGACCAAACT CCACAACATC
    ATTCCATTAC CTCAGACCGA TGGGGAACGT GAAGAGGTGG AGATGATCAA CTCTGGTGTT
    CAGAAGATAA GCAAAGACAC TGAACCAATT ACTTTAGCGA GCATCCAGTC TCCAGTAGAA
    AAAGACGACA CTGAGGTCAT CCCAACCCCA CTACCCAGCA CCAACCCCTT TGAGACCAAA
    TCTGATGAGG AGGCTGAAGA AGAGGAGGAA AACTCTCTAC AGGACCAGGC TGAAGACGAT
    GAAGCGTTTG TTGTGGCAGA TCTGAGCGAT CGACTGACGT TCCTGAAACA GCGCTCCAGA
    CTCGGCATCG CCATCGATGA GGACGAGGCC GAAGTCTTCG AGCTGCAGCC GCTCATAGCT
    GATGAAGCTC TCAGGACATT GGCGACATTG AGGCAACTCC TGATCAACCA CTTTTCCGGT
    CAGGAGGACA CAGACAGAGA GAACAAGAGT GAAGATCAAC AGGAGCATAT AGAGAGGACG
    TGCCCTCAGC AGGGCTCAGA CGACAGCGCT GCCATCCAGT CTCCGCTTGA GAACGGCTCA
    GACAGAGGAG AGAATCCACA GGAACTACCA TCCGGGGACA CCGGCTTCTT TGAGACGTCT
    GAAGATTGTG TGTCTGCAGG CAGTTATATG GTGCTGGAGG TGTTTGCGGG CTCAGGAGAG
    AGCAGCACAG ATGACGATGC GCAGGGCTGC AGTGTGGAGC CTGTGGCTGG TGGAGATTCA
    GGCATCGACT TGAAGAAGCT CCTGTCTTCA TCTTCTCAAA GCACTGGAGG ACCCAACATC
    AACAGACAGA TCATGACACA CTTACGTCTA CTACAGGGCA ACCTGCAGCA GCTGAAGGAG
    ACCGAAATCA AGTATAACCA GCTACGCCAA AGGCTATCAG AGGAAACAGC TACTGACACA
    GAGGAAAACA AAGATAAAAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa59289
    Clone ID Related Accession (Same CDS sequence) XM_017356362.2 , XM_017356362.1
    Accession Version XM_017356362.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4164bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG ATGAATCCAT ATCTCCGAGA CCAGGTGAAG GAGAGCTGGA GGATTTTAGT
    GGAGATGTGG ATCGACCCCC TTCCTGGCAG GGTGATGCTG GGACGGAGCC ATTATGGACC
    AACAATAACA TGACTCCAGT TACTCCTTGT CTTTTCCCAG AGAGCCCATG CCAAAGAGAG
    ACCCCCTGCC ACAGCCCAAA GACCACACCT CGAGACAGCC TCAACTCCTG TCCCTCGCCG
    GATGCAGAAG ACACACCTGA CCTAGATTCT CAGTCAAGTG TAGGGAGCCC TTGCTCTCGT
    CCTGTAGCAC ATATCATCGG TGCTGAGGAT GAAGACTTTG GCAAAGAGCA GGAACAGATG
    AATGGCCAGT GTAGCTGTTT TCAGAGCATA GACCTCCTGA AATCTCGACC TGCTCACCTG
    GCAGCTTTCA TCCATCACGT AGTCTCTCAG TTTGACCCTG CACCGCTGCT CTGCTACCTC
    TACGCTGATC TTTACAAACA ATCCAGCACC AAAGAGACAC GACGAATCCT CATGGATACC
    TTCTTCATAG ACAAAACAGC TAATCTGAAA GTAACAGTAC CTGAATCCAT CATCGCTGAC
    CTGGAGAGGA CCGCACACTC AACCAATGCA GAGCGGAAGA GGCCGGAGCA AATGTCTGAA
    GATGTACACC GACAATATGT GCAACAACTG CAGGACACTC TTCTAACTGA CGTTCAGAAG
    AACCTAGAAG ACTTTAGGCA CAAGCGTAGT ATGGGTCTGA CATTGGCTGA AGCTGAACTG
    GCCCGTCTGG ATACAGAGAG AATGAGAGAT CGTGTGGCTT TGGAAAAAGA GCGATCCTGT
    GCAGAGCACA TCCTTTCTAA GATAGAAGAA GTCCTATTGA CGTCACAAGC CACAGAGGAA
    GATAAGTGTA GCACCATGCA ATTTGTGATA CTATCGTATA TGAAACAGCT CGGTGTGAAA
    GTGAAGGAGT TGCGAGGGCT GGACCACAAA CCCAAGTTAA TGAATCCATT CCACAAAATA
    AAGAAAAGTA TGAAGCCTGA GAAGGATGGA AGTGAGGAGA AAGCGAAGAA AACACGATTT
    CCTAATGTCT TTCCTCAGAG ACGACCAAGC AAGATTGACG CTTTATCACA GCCGAAGCAG
    CGTACGCCAA AGCCTCAGCT GCCTCTGGGA ACTGGAGAGC AGGCAGACGG GTCGTCTCTT
    CCTGTACGGA GCAGTCAGTC CAGCGAGGGC TCAGATGGCA CCAATGCCCT GATCACATCA
    CCGCCTTACA GCGCTCCAGC AGGACAGGGC ACAGACGCCA TCAGCCGAGA GTCTGAGACT
    GGGGGACTTC CCTCTTCTGC CCTGCCCAAA TTGGGAGAGG GTACTGTCTC AGGGGATTTG
    CAGGACTCCT CATCTACAAA CACTCACTTT GACTTCAGCA ATTGCATGCT GGAGCATTTA
    CAGGAGGAAG AAGCTGAGAC TGAAAAGACG CAGGACTCTG GCACCCCCAG GCCTGACAGG
    AGCAGGATGG ATCCGCTGGG CTCTGGAGAG GTGCAGAGTG AAGATGATCA GTGTGTTGAA
    GCCGAACTCG AGCCTCCAAA CTGGCAGACA CTAGTGAGCA GAGACGTCCT GTCCACACTC
    ACGCCTCAGG AAATTAAGAG ACAAGAAGTC ATCAATGAGC TGTTCTACAC GGAGCGAGCA
    CACGTTCGAA TGATGAAGGT TCTGGAGAAT TTATTTTACC AGCCGCTGAT CAGAGACGCC
    ATCCTTCCTC CTGCAGACAT CAAAAACATC TTCAGCAACC TGGAGGAGAT CGTTCAGCTG
    CACTTGTCTT TAACCGAACA AATGGCCGCC GTACGGAAGA AGAACGAGAC CGCTCTCATT
    GACTCAATAG GAGATGATCT GCTTTCCTGG TTCAGTGAAG AAGAGGAGGA TAAGATCAAG
    AGAGCGGTGG GCACCTACTG CAGTAACCAG CCCTTCGCCC TGGAGCTCAT CAAGAGCAAA
    CAGAAGAAAG ACCAGCGCTT CAATGCCTTC ATACAGGAGA CAGAGAGCAA CCGTCATTGT
    CGCAGGCTGC AGTTGAAAGA CATTATTCCT GTGGAGACGT TACGACTCAC AAAGTATCCT
    CTTCTGCTGG AGAACATCGC CAAATACACA GAGGACTCTG AGGAGAGAAG TAAAGTGAAA
    CGGGCTGGCG AATGCTGTAG AAAGATCCTC ACTCACGTCA ACCAGGAGGT GAAGGAAGCG
    GAAAACAAGC AGAGGCTGGA GGACTACCAG AGAAGACTGG ACCTGTCCTC ACTGAAGCAG
    AGCGAAAACC CCATGATCGC AGAGTTCAAG AGCTTGGACT TGACCAAGAG GAAGATGGTG
    CACGAGGGGC CGCTGTCCTG GAAGGTCAAC AAAGACAAAA CCATTGAGCT GTACACGTTG
    CTGCTGGAGG ATATATTGGT GCTACTTCAA AGGCAGGATG AGAAGCTGAT CTTAAAATGC
    CACAGTAAGA ATCTGGCGGG CACCGCTGAG ACCAAACACA TCTTCAGCCC CATCATCAAG
    CTCAGCACTG TGATGGTGCG CTCCGTTGCC ACCGACAACA GGTCGTTCTT TGTGCTGTCG
    ATGTCTGATA ATGGAGCCCA GATCTATGAG CTGATGGCCC AGACCGTATC AGAGCAAAAG
    ACGTGGCAGT GTCTGATCAC ACAAAGAGCA GACTGCATGA AGACCAAACT CCACAACATC
    ATTCCATTAC CTCAGACCGA TGGGGAACGT GAAGAGGTGG AGATGATCAA CTCTGGTGTT
    CAGAAGATAA GCAAAGACAC TGAACCAATT ACTTTAGCGA GCATCCAGTC TCCAGTAGAA
    AAAGACGACA CTGAGGTCAT CCCAACCCCA CTACCCAGCA CCAACCCCTT TGAGACCAAA
    TCTGATGAGG AGGCTGAAGA AGAGGAGGAA AACTCTCTAC AGGACCAGGC TGAAGACGAT
    GAAGCGTTTG TTGTGGCAGA TCTGAGCGAT CGACTGACGT TCCTGAAACA GCGCTCCAGA
    CTCGGCATCG CCATCGATGA GGACGAGGCC GAAGTCTTCG AGCTGCAGCC GCTCATAGCT
    GATGAAGCTC TCAGGACATT GGCGACATTG AGGCAACTCC TGATCAACCA CTTTTCCGGT
    CAGGAGGACA CAGACAGAGA GAACAAGAGT GAAGATCAAC AGGAGCATAT AGAGAGGACG
    TGCCCTCAGC AGGGCTCAGA CGACAGCGCT GCCATCCAGT CTCCGCTTGA GAACGGCTCA
    GACAGAGGAG AGAATCCACA GGAACTACCA TCCGGGGACA CCGGCTTCTT TGAGACGTCT
    GAAGATTGTG TGTCTGCAGG CAGTTATATG GTGCTGGAGG TGTTTGCGGG CTCAGGAGAG
    AGCAGCACAG ATGACGATGC GCAGGGCTGC AGTGTGGAGC CTGTGGCTGG TGGAGATTCA
    GGCATCGACT TGAAGAAGCT CCTGTCTTCA TCTTCTCAAA GCACTGGAGG ACCCAACATC
    AACAGACAGA TCATGACACA CTTACGTCTA CTACAGGGCA ACCTGCAGCA GCTGAAGGAG
    ACCGAAATCA AGTATAACCA GCTACGCCAA AGGCTATCAG AGGAAACAGC TACTGACACA
    GAGGAAAACA AAGATAAAAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211851.1
    CDS1449..5612
    Translation

    Target ORF information:

    RefSeq Version XM_017356362.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG ATGAATCCAT ATCTCCGAGA CCAGGTGAAG GAGAGCTGGA GGATTTTAGT
    GGAGATGTGG ATCGACCCCC TTCCTGGCAG GGTGATGCTG GGACGGAGCC ATTATGGACC
    AACAATAACA TGACTCCAGT TACTCCTTGT CTTTTCCCAG AGAGCCCATG CCAAAGAGAG
    ACCCCCTGCC ACAGCCCAAA GACCACACCT CGAGACAGCC TCAACTCCTG TCCCTCGCCG
    GATGCAGAAG ACACACCTGA CCTAGATTCT CAGTCAAGTG TAGGGAGCCC TTGCTCTCGT
    CCTGTAGCAC ATATCATCGG TGCTGAGGAT GAAGACTTTG GCAAAGAGCA GGAACAGATG
    AATGGCCAGT GTAGCTGTTT TCAGAGCATA GACCTCCTGA AATCTCGACC TGCTCACCTG
    GCAGCTTTCA TCCATCACGT AGTCTCTCAG TTTGACCCTG CACCGCTGCT CTGCTACCTC
    TACGCTGATC TTTACAAACA ATCCAGCACC AAAGAGACAC GACGAATCCT CATGGATACC
    TTCTTCATAG ACAAAACAGC TAATCTGAAA GTAACAGTAC CTGAATCCAT CATCGCTGAC
    CTGGAGAGGA CCGCACACTC AACCAATGCA GAGCGGAAGA GGCCGGAGCA AATGTCTGAA
    GATGTACACC GACAATATGT GCAACAACTG CAGGACACTC TTCTAACTGA CGTTCAGAAG
    AACCTAGAAG ACTTTAGGCA CAAGCGTAGT ATGGGTCTGA CATTGGCTGA AGCTGAACTG
    GCCCGTCTGG ATACAGAGAG AATGAGAGAT CGTGTGGCTT TGGAAAAAGA GCGATCCTGT
    GCAGAGCACA TCCTTTCTAA GATAGAAGAA GTCCTATTGA CGTCACAAGC CACAGAGGAA
    GATAAGTGTA GCACCATGCA ATTTGTGATA CTATCGTATA TGAAACAGCT CGGTGTGAAA
    GTGAAGGAGT TGCGAGGGCT GGACCACAAA CCCAAGTTAA TGAATCCATT CCACAAAATA
    AAGAAAAGTA TGAAGCCTGA GAAGGATGGA AGTGAGGAGA AAGCGAAGAA AACACGATTT
    CCTAATGTCT TTCCTCAGAG ACGACCAAGC AAGATTGACG CTTTATCACA GCCGAAGCAG
    CGTACGCCAA AGCCTCAGCT GCCTCTGGGA ACTGGAGAGC AGGCAGACGG GTCGTCTCTT
    CCTGTACGGA GCAGTCAGTC CAGCGAGGGC TCAGATGGCA CCAATGCCCT GATCACATCA
    CCGCCTTACA GCGCTCCAGC AGGACAGGGC ACAGACGCCA TCAGCCGAGA GTCTGAGACT
    GGGGGACTTC CCTCTTCTGC CCTGCCCAAA TTGGGAGAGG GTACTGTCTC AGGGGATTTG
    CAGGACTCCT CATCTACAAA CACTCACTTT GACTTCAGCA ATTGCATGCT GGAGCATTTA
    CAGGAGGAAG AAGCTGAGAC TGAAAAGACG CAGGACTCTG GCACCCCCAG GCCTGACAGG
    AGCAGGATGG ATCCGCTGGG CTCTGGAGAG GTGCAGAGTG AAGATGATCA GTGTGTTGAA
    GCCGAACTCG AGCCTCCAAA CTGGCAGACA CTAGTGAGCA GAGACGTCCT GTCCACACTC
    ACGCCTCAGG AAATTAAGAG ACAAGAAGTC ATCAATGAGC TGTTCTACAC GGAGCGAGCA
    CACGTTCGAA TGATGAAGGT TCTGGAGAAT TTATTTTACC AGCCGCTGAT CAGAGACGCC
    ATCCTTCCTC CTGCAGACAT CAAAAACATC TTCAGCAACC TGGAGGAGAT CGTTCAGCTG
    CACTTGTCTT TAACCGAACA AATGGCCGCC GTACGGAAGA AGAACGAGAC CGCTCTCATT
    GACTCAATAG GAGATGATCT GCTTTCCTGG TTCAGTGAAG AAGAGGAGGA TAAGATCAAG
    AGAGCGGTGG GCACCTACTG CAGTAACCAG CCCTTCGCCC TGGAGCTCAT CAAGAGCAAA
    CAGAAGAAAG ACCAGCGCTT CAATGCCTTC ATACAGGAGA CAGAGAGCAA CCGTCATTGT
    CGCAGGCTGC AGTTGAAAGA CATTATTCCT GTGGAGACGT TACGACTCAC AAAGTATCCT
    CTTCTGCTGG AGAACATCGC CAAATACACA GAGGACTCTG AGGAGAGAAG TAAAGTGAAA
    CGGGCTGGCG AATGCTGTAG AAAGATCCTC ACTCACGTCA ACCAGGAGGT GAAGGAAGCG
    GAAAACAAGC AGAGGCTGGA GGACTACCAG AGAAGACTGG ACCTGTCCTC ACTGAAGCAG
    AGCGAAAACC CCATGATCGC AGAGTTCAAG AGCTTGGACT TGACCAAGAG GAAGATGGTG
    CACGAGGGGC CGCTGTCCTG GAAGGTCAAC AAAGACAAAA CCATTGAGCT GTACACGTTG
    CTGCTGGAGG ATATATTGGT GCTACTTCAA AGGCAGGATG AGAAGCTGAT CTTAAAATGC
    CACAGTAAGA ATCTGGCGGG CACCGCTGAG ACCAAACACA TCTTCAGCCC CATCATCAAG
    CTCAGCACTG TGATGGTGCG CTCCGTTGCC ACCGACAACA GGTCGTTCTT TGTGCTGTCG
    ATGTCTGATA ATGGAGCCCA GATCTATGAG CTGATGGCCC AGACCGTATC AGAGCAAAAG
    ACGTGGCAGT GTCTGATCAC ACAAAGAGCA GACTGCATGA AGACCAAACT CCACAACATC
    ATTCCATTAC CTCAGACCGA TGGGGAACGT GAAGAGGTGG AGATGATCAA CTCTGGTGTT
    CAGAAGATAA GCAAAGACAC TGAACCAATT ACTTTAGCGA GCATCCAGTC TCCAGTAGAA
    AAAGACGACA CTGAGGTCAT CCCAACCCCA CTACCCAGCA CCAACCCCTT TGAGACCAAA
    TCTGATGAGG AGGCTGAAGA AGAGGAGGAA AACTCTCTAC AGGACCAGGC TGAAGACGAT
    GAAGCGTTTG TTGTGGCAGA TCTGAGCGAT CGACTGACGT TCCTGAAACA GCGCTCCAGA
    CTCGGCATCG CCATCGATGA GGACGAGGCC GAAGTCTTCG AGCTGCAGCC GCTCATAGCT
    GATGAAGCTC TCAGGACATT GGCGACATTG AGGCAACTCC TGATCAACCA CTTTTCCGGT
    CAGGAGGACA CAGACAGAGA GAACAAGAGT GAAGATCAAC AGGAGCATAT AGAGAGGACG
    TGCCCTCAGC AGGGCTCAGA CGACAGCGCT GCCATCCAGT CTCCGCTTGA GAACGGCTCA
    GACAGAGGAG AGAATCCACA GGAACTACCA TCCGGGGACA CCGGCTTCTT TGAGACGTCT
    GAAGATTGTG TGTCTGCAGG CAGTTATATG GTGCTGGAGG TGTTTGCGGG CTCAGGAGAG
    AGCAGCACAG ATGACGATGC GCAGGGCTGC AGTGTGGAGC CTGTGGCTGG TGGAGATTCA
    GGCATCGACT TGAAGAAGCT CCTGTCTTCA TCTTCTCAAA GCACTGGAGG ACCCAACATC
    AACAGACAGA TCATGACACA CTTACGTCTA CTACAGGGCA ACCTGCAGCA GCTGAAGGAG
    ACCGAAATCA AGTATAACCA GCTACGCCAA AGGCTATCAG AGGAAACAGC TACTGACACA
    GAGGAAAACA AAGATAAAAG TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46069
    Clone ID Related Accession (Same CDS sequence) XM_009301885.2 , XM_009301885.3
    Accession Version XM_009301885.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG TAGAAAAAGA CGACACTGAG
    GTCATCCCAA CCCCACTACC CAGCACCAAC CCCTTTGAGA CCAAATCTGA TGAGGAGGCT
    GAAGAAGAGG AGGAAAACTC TCTACAGGAC CAGGCTGAAG ACGATGAAGC GTTTGTTGTG
    GCAGATCTGA GCGATCGACT GACGTTCCTG AAACAGCGCT CCAGACTCGG CATCGCCATC
    GATGAGGACG AGGCCGAAGT CTTCGAGCTG CAGCCGCTCA TAGCTGATGA AGCTCTCAGG
    ACATTGGCGA CATTGAGGCA ACTCCTGATC AACCACTTTT CCGGTCAGGA GGACACAGAC
    AGAGAGAACA AGAGTGAAGA TCAACAGGAG CATATAGAGA GGACGTGCCC TCAGCAGGGC
    TCAGACGACA GCGCTGCCAT CCAGTCTCCG CTTGAGAACG GCTCAGACAG AGGAGAGAAT
    CCACAGGAAC TACCATCCGG GGACACCGGC TTCTTTGAGA CGTCTGAAGA TTGTGTGTCT
    GCAGGCAGTT ATATGGTGCT GGAGGTGTTT GCGGGCTCAG GAGAGAGCAG CACAGATGAC
    GATGCGCAGG GCTGCAGTGT GGAGCCTGTG GCTGGTGGAG ATTCAGGCAT CGACTTGAAG
    AAGCTCCTGT CTTCATCTTC TCAAAGCACT GGAGGACCCA ACATCAACAG ACAGATCATG
    ACACACTTAC GTCTACTACA GGGCAACCTG CAGCAGCTGA AGGAGACCGA AATCAAGTAT
    AACCAGCTAC GCCAAAGGCT ATCAGAGGAA ACAGCTACTG ACACAGAGGA AAACAAAGAT
    AAAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300160.1
    CDS1449..5717
    Translation

    Target ORF information:

    RefSeq Version XM_009301885.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301885.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG TAGAAAAAGA CGACACTGAG
    GTCATCCCAA CCCCACTACC CAGCACCAAC CCCTTTGAGA CCAAATCTGA TGAGGAGGCT
    GAAGAAGAGG AGGAAAACTC TCTACAGGAC CAGGCTGAAG ACGATGAAGC GTTTGTTGTG
    GCAGATCTGA GCGATCGACT GACGTTCCTG AAACAGCGCT CCAGACTCGG CATCGCCATC
    GATGAGGACG AGGCCGAAGT CTTCGAGCTG CAGCCGCTCA TAGCTGATGA AGCTCTCAGG
    ACATTGGCGA CATTGAGGCA ACTCCTGATC AACCACTTTT CCGGTCAGGA GGACACAGAC
    AGAGAGAACA AGAGTGAAGA TCAACAGGAG CATATAGAGA GGACGTGCCC TCAGCAGGGC
    TCAGACGACA GCGCTGCCAT CCAGTCTCCG CTTGAGAACG GCTCAGACAG AGGAGAGAAT
    CCACAGGAAC TACCATCCGG GGACACCGGC TTCTTTGAGA CGTCTGAAGA TTGTGTGTCT
    GCAGGCAGTT ATATGGTGCT GGAGGTGTTT GCGGGCTCAG GAGAGAGCAG CACAGATGAC
    GATGCGCAGG GCTGCAGTGT GGAGCCTGTG GCTGGTGGAG ATTCAGGCAT CGACTTGAAG
    AAGCTCCTGT CTTCATCTTC TCAAAGCACT GGAGGACCCA ACATCAACAG ACAGATCATG
    ACACACTTAC GTCTACTACA GGGCAACCTG CAGCAGCTGA AGGAGACCGA AATCAAGTAT
    AACCAGCTAC GCCAAAGGCT ATCAGAGGAA ACAGCTACTG ACACAGAGGA AAACAAAGAT
    AAAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46069
    Clone ID Related Accession (Same CDS sequence) XM_009301885.2 , XM_009301885.3
    Accession Version XM_009301885.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301885.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG TAGAAAAAGA CGACACTGAG
    GTCATCCCAA CCCCACTACC CAGCACCAAC CCCTTTGAGA CCAAATCTGA TGAGGAGGCT
    GAAGAAGAGG AGGAAAACTC TCTACAGGAC CAGGCTGAAG ACGATGAAGC GTTTGTTGTG
    GCAGATCTGA GCGATCGACT GACGTTCCTG AAACAGCGCT CCAGACTCGG CATCGCCATC
    GATGAGGACG AGGCCGAAGT CTTCGAGCTG CAGCCGCTCA TAGCTGATGA AGCTCTCAGG
    ACATTGGCGA CATTGAGGCA ACTCCTGATC AACCACTTTT CCGGTCAGGA GGACACAGAC
    AGAGAGAACA AGAGTGAAGA TCAACAGGAG CATATAGAGA GGACGTGCCC TCAGCAGGGC
    TCAGACGACA GCGCTGCCAT CCAGTCTCCG CTTGAGAACG GCTCAGACAG AGGAGAGAAT
    CCACAGGAAC TACCATCCGG GGACACCGGC TTCTTTGAGA CGTCTGAAGA TTGTGTGTCT
    GCAGGCAGTT ATATGGTGCT GGAGGTGTTT GCGGGCTCAG GAGAGAGCAG CACAGATGAC
    GATGCGCAGG GCTGCAGTGT GGAGCCTGTG GCTGGTGGAG ATTCAGGCAT CGACTTGAAG
    AAGCTCCTGT CTTCATCTTC TCAAAGCACT GGAGGACCCA ACATCAACAG ACAGATCATG
    ACACACTTAC GTCTACTACA GGGCAACCTG CAGCAGCTGA AGGAGACCGA AATCAAGTAT
    AACCAGCTAC GCCAAAGGCT ATCAGAGGAA ACAGCTACTG ACACAGAGGA AAACAAAGAT
    AAAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300160.1
    CDS1368..5636
    Translation

    Target ORF information:

    RefSeq Version XM_009301885.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301885.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG TAGAAAAAGA CGACACTGAG
    GTCATCCCAA CCCCACTACC CAGCACCAAC CCCTTTGAGA CCAAATCTGA TGAGGAGGCT
    GAAGAAGAGG AGGAAAACTC TCTACAGGAC CAGGCTGAAG ACGATGAAGC GTTTGTTGTG
    GCAGATCTGA GCGATCGACT GACGTTCCTG AAACAGCGCT CCAGACTCGG CATCGCCATC
    GATGAGGACG AGGCCGAAGT CTTCGAGCTG CAGCCGCTCA TAGCTGATGA AGCTCTCAGG
    ACATTGGCGA CATTGAGGCA ACTCCTGATC AACCACTTTT CCGGTCAGGA GGACACAGAC
    AGAGAGAACA AGAGTGAAGA TCAACAGGAG CATATAGAGA GGACGTGCCC TCAGCAGGGC
    TCAGACGACA GCGCTGCCAT CCAGTCTCCG CTTGAGAACG GCTCAGACAG AGGAGAGAAT
    CCACAGGAAC TACCATCCGG GGACACCGGC TTCTTTGAGA CGTCTGAAGA TTGTGTGTCT
    GCAGGCAGTT ATATGGTGCT GGAGGTGTTT GCGGGCTCAG GAGAGAGCAG CACAGATGAC
    GATGCGCAGG GCTGCAGTGT GGAGCCTGTG GCTGGTGGAG ATTCAGGCAT CGACTTGAAG
    AAGCTCCTGT CTTCATCTTC TCAAAGCACT GGAGGACCCA ACATCAACAG ACAGATCATG
    ACACACTTAC GTCTACTACA GGGCAACCTG CAGCAGCTGA AGGAGACCGA AATCAAGTAT
    AACCAGCTAC GCCAAAGGCT ATCAGAGGAA ACAGCTACTG ACACAGAGGA AAACAAAGAT
    AAAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46070
    Clone ID Related Accession (Same CDS sequence) XM_009301886.2 , XM_009301886.3
    Accession Version XM_009301886.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301886.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG AAAAAGACGA CACTGAGGTC
    ATCCCAACCC CACTACCCAG CACCAACCCC TTTGAGACCA AATCTGATGA GGAGGCTGAA
    GAAGAGGAGG AAAACTCTCT ACAGGACCAG GCTGAAGACG ATGAAGCGTT TGTTGTGGCA
    GATCTGAGCG ATCGACTGAC GTTCCTGAAA CAGCGCTCCA GACTCGGCAT CGCCATCGAT
    GAGGACGAGG CCGAAGTCTT CGAGCTGCAG CCGCTCATAG CTGATGAAGC TCTCAGGACA
    TTGGCGACAT TGAGGCAACT CCTGATCAAC CACTTTTCCG GTCAGGAGGA CACAGACAGA
    GAGAACAAGA GTGAAGATCA ACAGGAGCAT ATAGAGAGGA CGTGCCCTCA GCAGGGCTCA
    GACGACAGCG CTGCCATCCA GTCTCCGCTT GAGAACGGCT CAGACAGAGG AGAGAATCCA
    CAGGAACTAC CATCCGGGGA CACCGGCTTC TTTGAGACGT CTGAAGATTG TGTGTCTGCA
    GGCAGTTATA TGGTGCTGGA GGTGTTTGCG GGCTCAGGAG AGAGCAGCAC AGATGACGAT
    GCGCAGGGCT GCAGTGTGGA GCCTGTGGCT GGTGGAGATT CAGGCATCGA CTTGAAGAAG
    CTCCTGTCTT CATCTTCTCA AAGCACTGGA GGACCCAACA TCAACAGACA GATCATGACA
    CACTTACGTC TACTACAGGG CAACCTGCAG CAGCTGAAGG AGACCGAAAT CAAGTATAAC
    CAGCTACGCC AAAGGCTATC AGAGGAAACA GCTACTGACA CAGAGGAAAA CAAAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300161.1
    CDS1450..5715
    Translation

    Target ORF information:

    RefSeq Version XM_009301886.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301886.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG AAAAAGACGA CACTGAGGTC
    ATCCCAACCC CACTACCCAG CACCAACCCC TTTGAGACCA AATCTGATGA GGAGGCTGAA
    GAAGAGGAGG AAAACTCTCT ACAGGACCAG GCTGAAGACG ATGAAGCGTT TGTTGTGGCA
    GATCTGAGCG ATCGACTGAC GTTCCTGAAA CAGCGCTCCA GACTCGGCAT CGCCATCGAT
    GAGGACGAGG CCGAAGTCTT CGAGCTGCAG CCGCTCATAG CTGATGAAGC TCTCAGGACA
    TTGGCGACAT TGAGGCAACT CCTGATCAAC CACTTTTCCG GTCAGGAGGA CACAGACAGA
    GAGAACAAGA GTGAAGATCA ACAGGAGCAT ATAGAGAGGA CGTGCCCTCA GCAGGGCTCA
    GACGACAGCG CTGCCATCCA GTCTCCGCTT GAGAACGGCT CAGACAGAGG AGAGAATCCA
    CAGGAACTAC CATCCGGGGA CACCGGCTTC TTTGAGACGT CTGAAGATTG TGTGTCTGCA
    GGCAGTTATA TGGTGCTGGA GGTGTTTGCG GGCTCAGGAG AGAGCAGCAC AGATGACGAT
    GCGCAGGGCT GCAGTGTGGA GCCTGTGGCT GGTGGAGATT CAGGCATCGA CTTGAAGAAG
    CTCCTGTCTT CATCTTCTCA AAGCACTGGA GGACCCAACA TCAACAGACA GATCATGACA
    CACTTACGTC TACTACAGGG CAACCTGCAG CAGCTGAAGG AGACCGAAAT CAAGTATAAC
    CAGCTACGCC AAAGGCTATC AGAGGAAACA GCTACTGACA CAGAGGAAAA CAAAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46070
    Clone ID Related Accession (Same CDS sequence) XM_009301886.2 , XM_009301886.3
    Accession Version XM_009301886.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4266bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301886.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG AAAAAGACGA CACTGAGGTC
    ATCCCAACCC CACTACCCAG CACCAACCCC TTTGAGACCA AATCTGATGA GGAGGCTGAA
    GAAGAGGAGG AAAACTCTCT ACAGGACCAG GCTGAAGACG ATGAAGCGTT TGTTGTGGCA
    GATCTGAGCG ATCGACTGAC GTTCCTGAAA CAGCGCTCCA GACTCGGCAT CGCCATCGAT
    GAGGACGAGG CCGAAGTCTT CGAGCTGCAG CCGCTCATAG CTGATGAAGC TCTCAGGACA
    TTGGCGACAT TGAGGCAACT CCTGATCAAC CACTTTTCCG GTCAGGAGGA CACAGACAGA
    GAGAACAAGA GTGAAGATCA ACAGGAGCAT ATAGAGAGGA CGTGCCCTCA GCAGGGCTCA
    GACGACAGCG CTGCCATCCA GTCTCCGCTT GAGAACGGCT CAGACAGAGG AGAGAATCCA
    CAGGAACTAC CATCCGGGGA CACCGGCTTC TTTGAGACGT CTGAAGATTG TGTGTCTGCA
    GGCAGTTATA TGGTGCTGGA GGTGTTTGCG GGCTCAGGAG AGAGCAGCAC AGATGACGAT
    GCGCAGGGCT GCAGTGTGGA GCCTGTGGCT GGTGGAGATT CAGGCATCGA CTTGAAGAAG
    CTCCTGTCTT CATCTTCTCA AAGCACTGGA GGACCCAACA TCAACAGACA GATCATGACA
    CACTTACGTC TACTACAGGG CAACCTGCAG CAGCTGAAGG AGACCGAAAT CAAGTATAAC
    CAGCTACGCC AAAGGCTATC AGAGGAAACA GCTACTGACA CAGAGGAAAA CAAAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300161.1
    CDS1369..5634
    Translation

    Target ORF information:

    RefSeq Version XM_009301886.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301886.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGTTTCCCA AAAAAGCGAG CAGGACAGGA 
    AGTATTCTCA GCAAGGACCA TCCCCCAGAT AAAAAGGCCA AAAGCGACAA GGCGTCAGTC
    CCCCTCTCGC ATGAGTTTGA CCCCACAGGG CTTGTGCAGC GTTGTGTTAT CATCCAGAAA
    GATGAGAATG GCTTTGGGCT GACAGTGAGC GGCGATAACC CTGTGTTTGT ACAGCTTGTA
    AAAGAGGATG GAGCTGCCAT GCGAGCTGGT GTTCAGACTG GTGACCGGAT CATAAAGGTC
    AATGGGACGT TAGTTACACA TTCAAATCAT GTAGAAGTTG TAAAGCTAAT AAAATCGGGC
    TCGTATGTGG CCCTGACGGT GCTGGGGAGA CCGCCTGGCT TGCCTCAGAT TCCCCTGTCA
    GACGGCGAGG GGGAAGAAGG GTCTCCGTTC TCGCTCAGTT CTCCTCACTC CCCAGGGCCC
    ATCGGACCAG AGCGCTCCTG TTCCTCCCCC AGCCCCTCAG AGCGAAAAGC AGCCTCTCTG
    CCTGTATGGG GCATAACAGA GGACTACAGA AACCCTACAT CTAAACTACT GAAGGAAATC
    CAGGACATTC CACAGAACCA GAACCAAGAG CAGCTCAGCA AACCCACTGG ACAGGATGAA
    TCCATATCTC CGAGACCAGG TGAAGGAGAG CTGGAGGATT TTAGTGGAGA TGTGGATCGA
    CCCCCTTCCT GGCAGGGTGA TGCTGGGACG GAGCCATTAT GGACCAACAA TAACATGACT
    CCAGTTACTC CTTGTCTTTT CCCAGAGAGC CCATGCCAAA GAGAGACCCC CTGCCACAGC
    CCAAAGACCA CACCTCGAGA CAGCCTCAAC TCCTGTCCCT CGCCGGATGC AGAAGACACA
    CCTGACCTAG ATTCTCAGTC AAGTGTAGGG AGCCCTTGCT CTCGTCCTGT AGCACATATC
    ATCGGTGCTG AGGATGAAGA CTTTGGCAAA GAGCAGGAAC AGATGAATGG CCAGTGTAGC
    TGTTTTCAGA GCATAGACCT CCTGAAATCT CGACCTGCTC ACCTGGCAGC TTTCATCCAT
    CACGTAGTCT CTCAGTTTGA CCCTGCACCG CTGCTCTGCT ACCTCTACGC TGATCTTTAC
    AAACAATCCA GCACCAAAGA GACACGACGA ATCCTCATGG ATACCTTCTT CATAGACAAA
    ACAGCTAATC TGAAAGTAAC AGTACCTGAA TCCATCATCG CTGACCTGGA GAGGACCGCA
    CACTCAACCA ATGCAGAGCG GAAGAGGCCG GAGCAAATGT CTGAAGATGT ACACCGACAA
    TATGTGCAAC AACTGCAGGA CACTCTTCTA ACTGACGTTC AGAAGAACCT AGAAGACTTT
    AGGCACAAGC GTAGTATGGG TCTGACATTG GCTGAAGCTG AACTGGCCCG TCTGGATACA
    GAGAGAATGA GAGATCGTGT GGCTTTGGAA AAAGAGCGAT CCTGTGCAGA GCACATCCTT
    TCTAAGATAG AAGAAGTCCT ATTGACGTCA CAAGCCACAG AGGAAGATAA GTGTAGCACC
    ATGCAATTTG TGATACTATC GTATATGAAA CAGCTCGGTG TGAAAGTGAA GGAGTTGCGA
    GGGCTGGACC ACAAACCCAA GTTAATGAAT CCATTCCACA AAATAAAGAA AAGTATGAAG
    CCTGAGAAGG ATGGAAGTGA GGAGAAAGCG AAGAAAACAC GATTTCCTAA TGTCTTTCCT
    CAGAGACGAC CAAGCAAGAT TGACGCTTTA TCACAGCCGA AGCAGCGTAC GCCAAAGCCT
    CAGCTGCCTC TGGGAACTGG AGAGCAGGCA GACGGGTCGT CTCTTCCTGT ACGGAGCAGT
    CAGTCCAGCG AGGGCTCAGA TGGCACCAAT GCCCTGATCA CATCACCGCC TTACAGCGCT
    CCAGCAGGAC AGGGCACAGA CGCCATCAGC CGAGAGTCTG AGACTGGGGG ACTTCCCTCT
    TCTGCCCTGC CCAAATTGGG AGAGGGTACT GTCTCAGGGG ATTTGCAGGA CTCCTCATCT
    ACAAACACTC ACTTTGACTT CAGCAATTGC ATGCTGGAGC ATTTACAGGA GGAAGAAGCT
    GAGACTGAAA AGACGCAGGA CTCTGGCACC CCCAGGCCTG ACAGGAGCAG GATGGATCCG
    CTGGGCTCTG GAGAGGTGCA GAGTGAAGAT GATCAGTGTG TTGAAGCCGA ACTCGAGCCT
    CCAAACTGGC AGACACTAGT GAGCAGAGAC GTCCTGTCCA CACTCACGCC TCAGGAAATT
    AAGAGACAAG AAGTCATCAA TGAGCTGTTC TACACGGAGC GAGCACACGT TCGAATGATG
    AAGGTTCTGG AGAATTTATT TTACCAGCCG CTGATCAGAG ACGCCATCCT TCCTCCTGCA
    GACATCAAAA ACATCTTCAG CAACCTGGAG GAGATCGTTC AGCTGCACTT GTCTTTAACC
    GAACAAATGG CCGCCGTACG GAAGAAGAAC GAGACCGCTC TCATTGACTC AATAGGAGAT
    GATCTGCTTT CCTGGTTCAG TGAAGAAGAG GAGGATAAGA TCAAGAGAGC GGTGGGCACC
    TACTGCAGTA ACCAGCCCTT CGCCCTGGAG CTCATCAAGA GCAAACAGAA GAAAGACCAG
    CGCTTCAATG CCTTCATACA GGAGACAGAG AGCAACCGTC ATTGTCGCAG GCTGCAGTTG
    AAAGACATTA TTCCTGTGGA GACGTTACGA CTCACAAAGT ATCCTCTTCT GCTGGAGAAC
    ATCGCCAAAT ACACAGAGGA CTCTGAGGAG AGAAGTAAAG TGAAACGGGC TGGCGAATGC
    TGTAGAAAGA TCCTCACTCA CGTCAACCAG GAGGTGAAGG AAGCGGAAAA CAAGCAGAGG
    CTGGAGGACT ACCAGAGAAG ACTGGACCTG TCCTCACTGA AGCAGAGCGA AAACCCCATG
    ATCGCAGAGT TCAAGAGCTT GGACTTGACC AAGAGGAAGA TGGTGCACGA GGGGCCGCTG
    TCCTGGAAGG TCAACAAAGA CAAAACCATT GAGCTGTACA CGTTGCTGCT GGAGGATATA
    TTGGTGCTAC TTCAAAGGCA GGATGAGAAG CTGATCTTAA AATGCCACAG TAAGAATCTG
    GCGGGCACCG CTGAGACCAA ACACATCTTC AGCCCCATCA TCAAGCTCAG CACTGTGATG
    GTGCGCTCCG TTGCCACCGA CAACAGGTCG TTCTTTGTGC TGTCGATGTC TGATAATGGA
    GCCCAGATCT ATGAGCTGAT GGCCCAGACC GTATCAGAGC AAAAGACGTG GCAGTGTCTG
    ATCACACAAA GAGCAGACTG CATGAAGACC AAACTCCACA ACATCATTCC ATTACCTCAG
    ACCGATGGGG AACGTGAAGA GGTGGAGATG ATCAACTCTG GTGTTCAGAA GATAAGCAAA
    GACACTGAAC CAATTACTTT AGCGAGCATC CAGTCTCCAG AAAAAGACGA CACTGAGGTC
    ATCCCAACCC CACTACCCAG CACCAACCCC TTTGAGACCA AATCTGATGA GGAGGCTGAA
    GAAGAGGAGG AAAACTCTCT ACAGGACCAG GCTGAAGACG ATGAAGCGTT TGTTGTGGCA
    GATCTGAGCG ATCGACTGAC GTTCCTGAAA CAGCGCTCCA GACTCGGCAT CGCCATCGAT
    GAGGACGAGG CCGAAGTCTT CGAGCTGCAG CCGCTCATAG CTGATGAAGC TCTCAGGACA
    TTGGCGACAT TGAGGCAACT CCTGATCAAC CACTTTTCCG GTCAGGAGGA CACAGACAGA
    GAGAACAAGA GTGAAGATCA ACAGGAGCAT ATAGAGAGGA CGTGCCCTCA GCAGGGCTCA
    GACGACAGCG CTGCCATCCA GTCTCCGCTT GAGAACGGCT CAGACAGAGG AGAGAATCCA
    CAGGAACTAC CATCCGGGGA CACCGGCTTC TTTGAGACGT CTGAAGATTG TGTGTCTGCA
    GGCAGTTATA TGGTGCTGGA GGTGTTTGCG GGCTCAGGAG AGAGCAGCAC AGATGACGAT
    GCGCAGGGCT GCAGTGTGGA GCCTGTGGCT GGTGGAGATT CAGGCATCGA CTTGAAGAAG
    CTCCTGTCTT CATCTTCTCA AAGCACTGGA GGACCCAACA TCAACAGACA GATCATGACA
    CACTTACGTC TACTACAGGG CAACCTGCAG CAGCTGAAGG AGACCGAAAT CAAGTATAAC
    CAGCTACGCC AAAGGCTATC AGAGGAAACA GCTACTGACA CAGAGGAAAA CAAAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46071
    Clone ID Related Accession (Same CDS sequence) XM_009301887.3 , XM_009301887.2
    Accession Version XM_009301887.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGACAGGAA GTATTCTCAG CAAGGACCAT 
    CCCCCAGATA AAAAGGCCAA AAGCGACAAG GCGTCAGTCC CCCTCTCGCA TGAGTTTGAC
    CCCACAGGGC TTGTGCAGCG TTGTGTTATC ATCCAGAAAG ATGAGAATGG CTTTGGGCTG
    ACAGTGAGCG GCGATAACCC TGTGTTTGTA CAGCTTGTAA AAGAGGATGG AGCTGCCATG
    CGAGCTGGTG TTCAGACTGG TGACCGGATC ATAAAGGTCA ATGGGACGTT AGTTACACAT
    TCAAATCATG TAGAAGTTGT AAAGCTAATA AAATCGGGCT CGTATGTGGC CCTGACGGTG
    CTGGGGAGAC CGCCTGGCTT GCCTCAGATT CCCCTGTCAG ACGGCGAGGG GGAAGAAGGG
    TCTCCGTTCT CGCTCAGTTC TCCTCACTCC CCAGGGCCCA TCGGACCAGA GCGCTCCTGT
    TCCTCCCCCA GCCCCTCAGA GCGAAAAGCA GCCTCTCTGC CTGTATGGGG CATAACAGAG
    GACTACAGAA ACCCTACATC TAAACTACTG AAGGAAATCC AGGACATTCC ACAGAACCAG
    AACCAAGAGC AGCTCAGCAA ACCCACTGGA CAGGATGAAT CCATATCTCC GAGACCAGGT
    GAAGGAGAGC TGGAGGATTT TAGTGGAGAT GTGGATCGAC CCCCTTCCTG GCAGGGTGAT
    GCTGGGACGG AGCCATTATG GACCAACAAT AACATGACTC CAGTTACTCC TTGTCTTTTC
    CCAGAGAGCC CATGCCAAAG AGAGACCCCC TGCCACAGCC CAAAGACCAC ACCTCGAGAC
    AGCCTCAACT CCTGTCCCTC GCCGGATGCA GAAGACACAC CTGACCTAGA TTCTCAGTCA
    AGTGTAGGGA GCCCTTGCTC TCGTCCTGTA GCACATATCA TCGGTGCTGA GGATGAAGAC
    TTTGGCAAAG AGCAGGAACA GATGAATGGC CAGTGTAGCT GTTTTCAGAG CATAGACCTC
    CTGAAATCTC GACCTGCTCA CCTGGCAGCT TTCATCCATC ACGTAGTCTC TCAGTTTGAC
    CCTGCACCGC TGCTCTGCTA CCTCTACGCT GATCTTTACA AACAATCCAG CACCAAAGAG
    ACACGACGAA TCCTCATGGA TACCTTCTTC ATAGACAAAA CAGCTAATCT GAAAGTAACA
    GTACCTGAAT CCATCATCGC TGACCTGGAG AGGACCGCAC ACTCAACCAA TGCAGAGCGG
    AAGAGGCCGG AGCAAATGTC TGAAGATGTA CACCGACAAT ATGTGCAACA ACTGCAGGAC
    ACTCTTCTAA CTGACGTTCA GAAGAACCTA GAAGACTTTA GGCACAAGCG TAGTATGGGT
    CTGACATTGG CTGAAGCTGA ACTGGCCCGT CTGGATACAG AGAGAATGAG AGATCGTGTG
    GCTTTGGAAA AAGAGCGATC CTGTGCAGAG CACATCCTTT CTAAGATAGA AGAAGTCCTA
    TTGACGTCAC AAGCCACAGA GGAAGATAAG TGTAGCACCA TGCAATTTGT GATACTATCG
    TATATGAAAC AGCTCGGTGT GAAAGTGAAG GAGTTGCGAG GGCTGGACCA CAAACCCAAG
    TTAATGAATC CATTCCACAA AATAAAGAAA AGTATGAAGC CTGAGAAGGA TGGAAGTGAG
    GAGAAAGCGA AGAAAACACG ATTTCCTAAT GTCTTTCCTC AGAGACGACC AAGCAAGATT
    GACGCTTTAT CACAGCCGAA GCAGCGTACG CCAAAGCCTC AGCTGCCTCT GGGAACTGGA
    GAGCAGGCAG ACGGGTCGTC TCTTCCTGTA CGGAGCAGTC AGTCCAGCGA GGGCTCAGAT
    GGCACCAATG CCCTGATCAC ATCACCGCCT TACAGCGCTC CAGCAGGACA GGGCACAGAC
    GCCATCAGCC GAGAGTCTGA GACTGGGGGA CTTCCCTCTT CTGCCCTGCC CAAATTGGGA
    GAGGGTACTG TCTCAGGGGA TTTGCAGGAC TCCTCATCTA CAAACACTCA CTTTGACTTC
    AGCAATTGCA TGCTGGAGCA TTTACAGGAG GAAGAAGCTG AGACTGAAAA GACGCAGGAC
    TCTGGCACCC CCAGGCCTGA CAGGAGCAGG ATGGATCCGC TGGGCTCTGG AGAGGTGCAG
    AGTGAAGATG ATCAGTGTGT TGAAGCCGAA CTCGAGCCTC CAAACTGGCA GACACTAGTG
    AGCAGAGACG TCCTGTCCAC ACTCACGCCT CAGGAAATTA AGAGACAAGA AGTCATCAAT
    GAGCTGTTCT ACACGGAGCG AGCACACGTT CGAATGATGA AGGTTCTGGA GAATTTATTT
    TACCAGCCGC TGATCAGAGA CGCCATCCTT CCTCCTGCAG ACATCAAAAA CATCTTCAGC
    AACCTGGAGG AGATCGTTCA GCTGCACTTG TCTTTAACCG AACAAATGGC CGCCGTACGG
    AAGAAGAACG AGACCGCTCT CATTGACTCA ATAGGAGATG ATCTGCTTTC CTGGTTCAGT
    GAAGAAGAGG AGGATAAGAT CAAGAGAGCG GTGGGCACCT ACTGCAGTAA CCAGCCCTTC
    GCCCTGGAGC TCATCAAGAG CAAACAGAAG AAAGACCAGC GCTTCAATGC CTTCATACAG
    GAGACAGAGA GCAACCGTCA TTGTCGCAGG CTGCAGTTGA AAGACATTAT TCCTGTGGAG
    ACGTTACGAC TCACAAAGTA TCCTCTTCTG CTGGAGAACA TCGCCAAATA CACAGAGGAC
    TCTGAGGAGA GAAGTAAAGT GAAACGGGCT GGCGAATGCT GTAGAAAGAT CCTCACTCAC
    GTCAACCAGG AGGTGAAGGA AGCGGAAAAC AAGCAGAGGC TGGAGGACTA CCAGAGAAGA
    CTGGACCTGT CCTCACTGAA GCAGAGCGAA AACCCCATGA TCGCAGAGTT CAAGAGCTTG
    GACTTGACCA AGAGGAAGAT GGTGCACGAG GGGCCGCTGT CCTGGAAGGT CAACAAAGAC
    AAAACCATTG AGCTGTACAC GTTGCTGCTG GAGGATATAT TGGTGCTACT TCAAAGGCAG
    GATGAGAAGC TGATCTTAAA ATGCCACAGT AAGAATCTGG CGGGCACCGC TGAGACCAAA
    CACATCTTCA GCCCCATCAT CAAGCTCAGC ACTGTGATGG TGCGCTCCGT TGCCACCGAC
    AACAGGTCGT TCTTTGTGCT GTCGATGTCT GATAATGGAG CCCAGATCTA TGAGCTGATG
    GCCCAGACCG TATCAGAGCA AAAGACGTGG CAGTGTCTGA TCACACAAAG AGCAGACTGC
    ATGAAGACCA AACTCCACAA CATCATTCCA TTACCTCAGA CCGATGGGGA ACGTGAAGAG
    GTGGAGATGA TCAACTCTGG TGTTCAGAAG ATAAGCAAAG ACACTGAACC AATTACTTTA
    GCGAGCATCC AGTCTCCAGT AGAAAAAGAC GACACTGAGG TCATCCCAAC CCCACTACCC
    AGCACCAACC CCTTTGAGAC CAAATCTGAT GAGGAGGCTG AAGAAGAGGA GGAAAACTCT
    CTACAGGACC AGGCTGAAGA CGATGAAGCG TTTGTTGTGG CAGATCTGAG CGATCGACTG
    ACGTTCCTGA AACAGCGCTC CAGACTCGGC ATCGCCATCG ATGAGGACGA GGCCGAAGTC
    TTCGAGCTGC AGCCGCTCAT AGCTGATGAA GCTCTCAGGA CATTGGCGAC ATTGAGGCAA
    CTCCTGATCA ACCACTTTTC CGGTCAGGAG GACACAGACA GAGAGAACAA GAGTGAAGAT
    CAACAGGAGC ATATAGAGAG GACGTGCCCT CAGCAGGGCT CAGACGACAG CGCTGCCATC
    CAGTCTCCGC TTGAGAACGG CTCAGACAGA GGAGAGAATC CACAGGAACT ACCATCCGGG
    GACACCGGCT TCTTTGAGAC GTCTGAAGAT TGTGTGTCTG CAGGCAGTTA TATGGTGCTG
    GAGGTGTTTG CGGGCTCAGG AGAGAGCAGC ACAGATGACG ATGCGCAGGG CTGCAGTGTG
    GAGCCTGTGG CTGGTGGAGA TTCAGGCATC GACTTGAAGA AGCTCCTGTC TTCATCTTCT
    CAAAGCACTG GAGGACCCAA CATCAACAGA CAGATCATGA CACACTTACG TCTACTACAG
    GGCAACCTGC AGCAGCTGAA GGAGACCGAA ATCAAGTATA ACCAGCTACG CCAAAGGCTA
    TCAGAGGAAA CAGCTACTGA CACAGAGGAA AACAAAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300162.1
    CDS1368..5615
    Translation

    Target ORF information:

    RefSeq Version XM_009301887.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301887.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGACAGGAA GTATTCTCAG CAAGGACCAT 
    CCCCCAGATA AAAAGGCCAA AAGCGACAAG GCGTCAGTCC CCCTCTCGCA TGAGTTTGAC
    CCCACAGGGC TTGTGCAGCG TTGTGTTATC ATCCAGAAAG ATGAGAATGG CTTTGGGCTG
    ACAGTGAGCG GCGATAACCC TGTGTTTGTA CAGCTTGTAA AAGAGGATGG AGCTGCCATG
    CGAGCTGGTG TTCAGACTGG TGACCGGATC ATAAAGGTCA ATGGGACGTT AGTTACACAT
    TCAAATCATG TAGAAGTTGT AAAGCTAATA AAATCGGGCT CGTATGTGGC CCTGACGGTG
    CTGGGGAGAC CGCCTGGCTT GCCTCAGATT CCCCTGTCAG ACGGCGAGGG GGAAGAAGGG
    TCTCCGTTCT CGCTCAGTTC TCCTCACTCC CCAGGGCCCA TCGGACCAGA GCGCTCCTGT
    TCCTCCCCCA GCCCCTCAGA GCGAAAAGCA GCCTCTCTGC CTGTATGGGG CATAACAGAG
    GACTACAGAA ACCCTACATC TAAACTACTG AAGGAAATCC AGGACATTCC ACAGAACCAG
    AACCAAGAGC AGCTCAGCAA ACCCACTGGA CAGGATGAAT CCATATCTCC GAGACCAGGT
    GAAGGAGAGC TGGAGGATTT TAGTGGAGAT GTGGATCGAC CCCCTTCCTG GCAGGGTGAT
    GCTGGGACGG AGCCATTATG GACCAACAAT AACATGACTC CAGTTACTCC TTGTCTTTTC
    CCAGAGAGCC CATGCCAAAG AGAGACCCCC TGCCACAGCC CAAAGACCAC ACCTCGAGAC
    AGCCTCAACT CCTGTCCCTC GCCGGATGCA GAAGACACAC CTGACCTAGA TTCTCAGTCA
    AGTGTAGGGA GCCCTTGCTC TCGTCCTGTA GCACATATCA TCGGTGCTGA GGATGAAGAC
    TTTGGCAAAG AGCAGGAACA GATGAATGGC CAGTGTAGCT GTTTTCAGAG CATAGACCTC
    CTGAAATCTC GACCTGCTCA CCTGGCAGCT TTCATCCATC ACGTAGTCTC TCAGTTTGAC
    CCTGCACCGC TGCTCTGCTA CCTCTACGCT GATCTTTACA AACAATCCAG CACCAAAGAG
    ACACGACGAA TCCTCATGGA TACCTTCTTC ATAGACAAAA CAGCTAATCT GAAAGTAACA
    GTACCTGAAT CCATCATCGC TGACCTGGAG AGGACCGCAC ACTCAACCAA TGCAGAGCGG
    AAGAGGCCGG AGCAAATGTC TGAAGATGTA CACCGACAAT ATGTGCAACA ACTGCAGGAC
    ACTCTTCTAA CTGACGTTCA GAAGAACCTA GAAGACTTTA GGCACAAGCG TAGTATGGGT
    CTGACATTGG CTGAAGCTGA ACTGGCCCGT CTGGATACAG AGAGAATGAG AGATCGTGTG
    GCTTTGGAAA AAGAGCGATC CTGTGCAGAG CACATCCTTT CTAAGATAGA AGAAGTCCTA
    TTGACGTCAC AAGCCACAGA GGAAGATAAG TGTAGCACCA TGCAATTTGT GATACTATCG
    TATATGAAAC AGCTCGGTGT GAAAGTGAAG GAGTTGCGAG GGCTGGACCA CAAACCCAAG
    TTAATGAATC CATTCCACAA AATAAAGAAA AGTATGAAGC CTGAGAAGGA TGGAAGTGAG
    GAGAAAGCGA AGAAAACACG ATTTCCTAAT GTCTTTCCTC AGAGACGACC AAGCAAGATT
    GACGCTTTAT CACAGCCGAA GCAGCGTACG CCAAAGCCTC AGCTGCCTCT GGGAACTGGA
    GAGCAGGCAG ACGGGTCGTC TCTTCCTGTA CGGAGCAGTC AGTCCAGCGA GGGCTCAGAT
    GGCACCAATG CCCTGATCAC ATCACCGCCT TACAGCGCTC CAGCAGGACA GGGCACAGAC
    GCCATCAGCC GAGAGTCTGA GACTGGGGGA CTTCCCTCTT CTGCCCTGCC CAAATTGGGA
    GAGGGTACTG TCTCAGGGGA TTTGCAGGAC TCCTCATCTA CAAACACTCA CTTTGACTTC
    AGCAATTGCA TGCTGGAGCA TTTACAGGAG GAAGAAGCTG AGACTGAAAA GACGCAGGAC
    TCTGGCACCC CCAGGCCTGA CAGGAGCAGG ATGGATCCGC TGGGCTCTGG AGAGGTGCAG
    AGTGAAGATG ATCAGTGTGT TGAAGCCGAA CTCGAGCCTC CAAACTGGCA GACACTAGTG
    AGCAGAGACG TCCTGTCCAC ACTCACGCCT CAGGAAATTA AGAGACAAGA AGTCATCAAT
    GAGCTGTTCT ACACGGAGCG AGCACACGTT CGAATGATGA AGGTTCTGGA GAATTTATTT
    TACCAGCCGC TGATCAGAGA CGCCATCCTT CCTCCTGCAG ACATCAAAAA CATCTTCAGC
    AACCTGGAGG AGATCGTTCA GCTGCACTTG TCTTTAACCG AACAAATGGC CGCCGTACGG
    AAGAAGAACG AGACCGCTCT CATTGACTCA ATAGGAGATG ATCTGCTTTC CTGGTTCAGT
    GAAGAAGAGG AGGATAAGAT CAAGAGAGCG GTGGGCACCT ACTGCAGTAA CCAGCCCTTC
    GCCCTGGAGC TCATCAAGAG CAAACAGAAG AAAGACCAGC GCTTCAATGC CTTCATACAG
    GAGACAGAGA GCAACCGTCA TTGTCGCAGG CTGCAGTTGA AAGACATTAT TCCTGTGGAG
    ACGTTACGAC TCACAAAGTA TCCTCTTCTG CTGGAGAACA TCGCCAAATA CACAGAGGAC
    TCTGAGGAGA GAAGTAAAGT GAAACGGGCT GGCGAATGCT GTAGAAAGAT CCTCACTCAC
    GTCAACCAGG AGGTGAAGGA AGCGGAAAAC AAGCAGAGGC TGGAGGACTA CCAGAGAAGA
    CTGGACCTGT CCTCACTGAA GCAGAGCGAA AACCCCATGA TCGCAGAGTT CAAGAGCTTG
    GACTTGACCA AGAGGAAGAT GGTGCACGAG GGGCCGCTGT CCTGGAAGGT CAACAAAGAC
    AAAACCATTG AGCTGTACAC GTTGCTGCTG GAGGATATAT TGGTGCTACT TCAAAGGCAG
    GATGAGAAGC TGATCTTAAA ATGCCACAGT AAGAATCTGG CGGGCACCGC TGAGACCAAA
    CACATCTTCA GCCCCATCAT CAAGCTCAGC ACTGTGATGG TGCGCTCCGT TGCCACCGAC
    AACAGGTCGT TCTTTGTGCT GTCGATGTCT GATAATGGAG CCCAGATCTA TGAGCTGATG
    GCCCAGACCG TATCAGAGCA AAAGACGTGG CAGTGTCTGA TCACACAAAG AGCAGACTGC
    ATGAAGACCA AACTCCACAA CATCATTCCA TTACCTCAGA CCGATGGGGA ACGTGAAGAG
    GTGGAGATGA TCAACTCTGG TGTTCAGAAG ATAAGCAAAG ACACTGAACC AATTACTTTA
    GCGAGCATCC AGTCTCCAGT AGAAAAAGAC GACACTGAGG TCATCCCAAC CCCACTACCC
    AGCACCAACC CCTTTGAGAC CAAATCTGAT GAGGAGGCTG AAGAAGAGGA GGAAAACTCT
    CTACAGGACC AGGCTGAAGA CGATGAAGCG TTTGTTGTGG CAGATCTGAG CGATCGACTG
    ACGTTCCTGA AACAGCGCTC CAGACTCGGC ATCGCCATCG ATGAGGACGA GGCCGAAGTC
    TTCGAGCTGC AGCCGCTCAT AGCTGATGAA GCTCTCAGGA CATTGGCGAC ATTGAGGCAA
    CTCCTGATCA ACCACTTTTC CGGTCAGGAG GACACAGACA GAGAGAACAA GAGTGAAGAT
    CAACAGGAGC ATATAGAGAG GACGTGCCCT CAGCAGGGCT CAGACGACAG CGCTGCCATC
    CAGTCTCCGC TTGAGAACGG CTCAGACAGA GGAGAGAATC CACAGGAACT ACCATCCGGG
    GACACCGGCT TCTTTGAGAC GTCTGAAGAT TGTGTGTCTG CAGGCAGTTA TATGGTGCTG
    GAGGTGTTTG CGGGCTCAGG AGAGAGCAGC ACAGATGACG ATGCGCAGGG CTGCAGTGTG
    GAGCCTGTGG CTGGTGGAGA TTCAGGCATC GACTTGAAGA AGCTCCTGTC TTCATCTTCT
    CAAAGCACTG GAGGACCCAA CATCAACAGA CAGATCATGA CACACTTACG TCTACTACAG
    GGCAACCTGC AGCAGCTGAA GGAGACCGAA ATCAAGTATA ACCAGCTACG CCAAAGGCTA
    TCAGAGGAAA CAGCTACTGA CACAGAGGAA AACAAAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46071
    Clone ID Related Accession (Same CDS sequence) XM_009301887.3 , XM_009301887.2
    Accession Version XM_009301887.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4248bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGACAGGAA GTATTCTCAG CAAGGACCAT 
    CCCCCAGATA AAAAGGCCAA AAGCGACAAG GCGTCAGTCC CCCTCTCGCA TGAGTTTGAC
    CCCACAGGGC TTGTGCAGCG TTGTGTTATC ATCCAGAAAG ATGAGAATGG CTTTGGGCTG
    ACAGTGAGCG GCGATAACCC TGTGTTTGTA CAGCTTGTAA AAGAGGATGG AGCTGCCATG
    CGAGCTGGTG TTCAGACTGG TGACCGGATC ATAAAGGTCA ATGGGACGTT AGTTACACAT
    TCAAATCATG TAGAAGTTGT AAAGCTAATA AAATCGGGCT CGTATGTGGC CCTGACGGTG
    CTGGGGAGAC CGCCTGGCTT GCCTCAGATT CCCCTGTCAG ACGGCGAGGG GGAAGAAGGG
    TCTCCGTTCT CGCTCAGTTC TCCTCACTCC CCAGGGCCCA TCGGACCAGA GCGCTCCTGT
    TCCTCCCCCA GCCCCTCAGA GCGAAAAGCA GCCTCTCTGC CTGTATGGGG CATAACAGAG
    GACTACAGAA ACCCTACATC TAAACTACTG AAGGAAATCC AGGACATTCC ACAGAACCAG
    AACCAAGAGC AGCTCAGCAA ACCCACTGGA CAGGATGAAT CCATATCTCC GAGACCAGGT
    GAAGGAGAGC TGGAGGATTT TAGTGGAGAT GTGGATCGAC CCCCTTCCTG GCAGGGTGAT
    GCTGGGACGG AGCCATTATG GACCAACAAT AACATGACTC CAGTTACTCC TTGTCTTTTC
    CCAGAGAGCC CATGCCAAAG AGAGACCCCC TGCCACAGCC CAAAGACCAC ACCTCGAGAC
    AGCCTCAACT CCTGTCCCTC GCCGGATGCA GAAGACACAC CTGACCTAGA TTCTCAGTCA
    AGTGTAGGGA GCCCTTGCTC TCGTCCTGTA GCACATATCA TCGGTGCTGA GGATGAAGAC
    TTTGGCAAAG AGCAGGAACA GATGAATGGC CAGTGTAGCT GTTTTCAGAG CATAGACCTC
    CTGAAATCTC GACCTGCTCA CCTGGCAGCT TTCATCCATC ACGTAGTCTC TCAGTTTGAC
    CCTGCACCGC TGCTCTGCTA CCTCTACGCT GATCTTTACA AACAATCCAG CACCAAAGAG
    ACACGACGAA TCCTCATGGA TACCTTCTTC ATAGACAAAA CAGCTAATCT GAAAGTAACA
    GTACCTGAAT CCATCATCGC TGACCTGGAG AGGACCGCAC ACTCAACCAA TGCAGAGCGG
    AAGAGGCCGG AGCAAATGTC TGAAGATGTA CACCGACAAT ATGTGCAACA ACTGCAGGAC
    ACTCTTCTAA CTGACGTTCA GAAGAACCTA GAAGACTTTA GGCACAAGCG TAGTATGGGT
    CTGACATTGG CTGAAGCTGA ACTGGCCCGT CTGGATACAG AGAGAATGAG AGATCGTGTG
    GCTTTGGAAA AAGAGCGATC CTGTGCAGAG CACATCCTTT CTAAGATAGA AGAAGTCCTA
    TTGACGTCAC AAGCCACAGA GGAAGATAAG TGTAGCACCA TGCAATTTGT GATACTATCG
    TATATGAAAC AGCTCGGTGT GAAAGTGAAG GAGTTGCGAG GGCTGGACCA CAAACCCAAG
    TTAATGAATC CATTCCACAA AATAAAGAAA AGTATGAAGC CTGAGAAGGA TGGAAGTGAG
    GAGAAAGCGA AGAAAACACG ATTTCCTAAT GTCTTTCCTC AGAGACGACC AAGCAAGATT
    GACGCTTTAT CACAGCCGAA GCAGCGTACG CCAAAGCCTC AGCTGCCTCT GGGAACTGGA
    GAGCAGGCAG ACGGGTCGTC TCTTCCTGTA CGGAGCAGTC AGTCCAGCGA GGGCTCAGAT
    GGCACCAATG CCCTGATCAC ATCACCGCCT TACAGCGCTC CAGCAGGACA GGGCACAGAC
    GCCATCAGCC GAGAGTCTGA GACTGGGGGA CTTCCCTCTT CTGCCCTGCC CAAATTGGGA
    GAGGGTACTG TCTCAGGGGA TTTGCAGGAC TCCTCATCTA CAAACACTCA CTTTGACTTC
    AGCAATTGCA TGCTGGAGCA TTTACAGGAG GAAGAAGCTG AGACTGAAAA GACGCAGGAC
    TCTGGCACCC CCAGGCCTGA CAGGAGCAGG ATGGATCCGC TGGGCTCTGG AGAGGTGCAG
    AGTGAAGATG ATCAGTGTGT TGAAGCCGAA CTCGAGCCTC CAAACTGGCA GACACTAGTG
    AGCAGAGACG TCCTGTCCAC ACTCACGCCT CAGGAAATTA AGAGACAAGA AGTCATCAAT
    GAGCTGTTCT ACACGGAGCG AGCACACGTT CGAATGATGA AGGTTCTGGA GAATTTATTT
    TACCAGCCGC TGATCAGAGA CGCCATCCTT CCTCCTGCAG ACATCAAAAA CATCTTCAGC
    AACCTGGAGG AGATCGTTCA GCTGCACTTG TCTTTAACCG AACAAATGGC CGCCGTACGG
    AAGAAGAACG AGACCGCTCT CATTGACTCA ATAGGAGATG ATCTGCTTTC CTGGTTCAGT
    GAAGAAGAGG AGGATAAGAT CAAGAGAGCG GTGGGCACCT ACTGCAGTAA CCAGCCCTTC
    GCCCTGGAGC TCATCAAGAG CAAACAGAAG AAAGACCAGC GCTTCAATGC CTTCATACAG
    GAGACAGAGA GCAACCGTCA TTGTCGCAGG CTGCAGTTGA AAGACATTAT TCCTGTGGAG
    ACGTTACGAC TCACAAAGTA TCCTCTTCTG CTGGAGAACA TCGCCAAATA CACAGAGGAC
    TCTGAGGAGA GAAGTAAAGT GAAACGGGCT GGCGAATGCT GTAGAAAGAT CCTCACTCAC
    GTCAACCAGG AGGTGAAGGA AGCGGAAAAC AAGCAGAGGC TGGAGGACTA CCAGAGAAGA
    CTGGACCTGT CCTCACTGAA GCAGAGCGAA AACCCCATGA TCGCAGAGTT CAAGAGCTTG
    GACTTGACCA AGAGGAAGAT GGTGCACGAG GGGCCGCTGT CCTGGAAGGT CAACAAAGAC
    AAAACCATTG AGCTGTACAC GTTGCTGCTG GAGGATATAT TGGTGCTACT TCAAAGGCAG
    GATGAGAAGC TGATCTTAAA ATGCCACAGT AAGAATCTGG CGGGCACCGC TGAGACCAAA
    CACATCTTCA GCCCCATCAT CAAGCTCAGC ACTGTGATGG TGCGCTCCGT TGCCACCGAC
    AACAGGTCGT TCTTTGTGCT GTCGATGTCT GATAATGGAG CCCAGATCTA TGAGCTGATG
    GCCCAGACCG TATCAGAGCA AAAGACGTGG CAGTGTCTGA TCACACAAAG AGCAGACTGC
    ATGAAGACCA AACTCCACAA CATCATTCCA TTACCTCAGA CCGATGGGGA ACGTGAAGAG
    GTGGAGATGA TCAACTCTGG TGTTCAGAAG ATAAGCAAAG ACACTGAACC AATTACTTTA
    GCGAGCATCC AGTCTCCAGT AGAAAAAGAC GACACTGAGG TCATCCCAAC CCCACTACCC
    AGCACCAACC CCTTTGAGAC CAAATCTGAT GAGGAGGCTG AAGAAGAGGA GGAAAACTCT
    CTACAGGACC AGGCTGAAGA CGATGAAGCG TTTGTTGTGG CAGATCTGAG CGATCGACTG
    ACGTTCCTGA AACAGCGCTC CAGACTCGGC ATCGCCATCG ATGAGGACGA GGCCGAAGTC
    TTCGAGCTGC AGCCGCTCAT AGCTGATGAA GCTCTCAGGA CATTGGCGAC ATTGAGGCAA
    CTCCTGATCA ACCACTTTTC CGGTCAGGAG GACACAGACA GAGAGAACAA GAGTGAAGAT
    CAACAGGAGC ATATAGAGAG GACGTGCCCT CAGCAGGGCT CAGACGACAG CGCTGCCATC
    CAGTCTCCGC TTGAGAACGG CTCAGACAGA GGAGAGAATC CACAGGAACT ACCATCCGGG
    GACACCGGCT TCTTTGAGAC GTCTGAAGAT TGTGTGTCTG CAGGCAGTTA TATGGTGCTG
    GAGGTGTTTG CGGGCTCAGG AGAGAGCAGC ACAGATGACG ATGCGCAGGG CTGCAGTGTG
    GAGCCTGTGG CTGGTGGAGA TTCAGGCATC GACTTGAAGA AGCTCCTGTC TTCATCTTCT
    CAAAGCACTG GAGGACCCAA CATCAACAGA CAGATCATGA CACACTTACG TCTACTACAG
    GGCAACCTGC AGCAGCTGAA GGAGACCGAA ATCAAGTATA ACCAGCTACG CCAAAGGCTA
    TCAGAGGAAA CAGCTACTGA CACAGAGGAA AACAAAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300162.1
    CDS1449..5696
    Translation

    Target ORF information:

    RefSeq Version XM_009301887.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 12b (arhgef12b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009301887.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGCGGCA CACAGTCCAC GCTTGCTGAG AGGACAGGAA GTATTCTCAG CAAGGACCAT 
    CCCCCAGATA AAAAGGCCAA AAGCGACAAG GCGTCAGTCC CCCTCTCGCA TGAGTTTGAC
    CCCACAGGGC TTGTGCAGCG TTGTGTTATC ATCCAGAAAG ATGAGAATGG CTTTGGGCTG
    ACAGTGAGCG GCGATAACCC TGTGTTTGTA CAGCTTGTAA AAGAGGATGG AGCTGCCATG
    CGAGCTGGTG TTCAGACTGG TGACCGGATC ATAAAGGTCA ATGGGACGTT AGTTACACAT
    TCAAATCATG TAGAAGTTGT AAAGCTAATA AAATCGGGCT CGTATGTGGC CCTGACGGTG
    CTGGGGAGAC CGCCTGGCTT GCCTCAGATT CCCCTGTCAG ACGGCGAGGG GGAAGAAGGG
    TCTCCGTTCT CGCTCAGTTC TCCTCACTCC CCAGGGCCCA TCGGACCAGA GCGCTCCTGT
    TCCTCCCCCA GCCCCTCAGA GCGAAAAGCA GCCTCTCTGC CTGTATGGGG CATAACAGAG
    GACTACAGAA ACCCTACATC TAAACTACTG AAGGAAATCC AGGACATTCC ACAGAACCAG
    AACCAAGAGC AGCTCAGCAA ACCCACTGGA CAGGATGAAT CCATATCTCC GAGACCAGGT
    GAAGGAGAGC TGGAGGATTT TAGTGGAGAT GTGGATCGAC CCCCTTCCTG GCAGGGTGAT
    GCTGGGACGG AGCCATTATG GACCAACAAT AACATGACTC CAGTTACTCC TTGTCTTTTC
    CCAGAGAGCC CATGCCAAAG AGAGACCCCC TGCCACAGCC CAAAGACCAC ACCTCGAGAC
    AGCCTCAACT CCTGTCCCTC GCCGGATGCA GAAGACACAC CTGACCTAGA TTCTCAGTCA
    AGTGTAGGGA GCCCTTGCTC TCGTCCTGTA GCACATATCA TCGGTGCTGA GGATGAAGAC
    TTTGGCAAAG AGCAGGAACA GATGAATGGC CAGTGTAGCT GTTTTCAGAG CATAGACCTC
    CTGAAATCTC GACCTGCTCA CCTGGCAGCT TTCATCCATC ACGTAGTCTC TCAGTTTGAC
    CCTGCACCGC TGCTCTGCTA CCTCTACGCT GATCTTTACA AACAATCCAG CACCAAAGAG
    ACACGACGAA TCCTCATGGA TACCTTCTTC ATAGACAAAA CAGCTAATCT GAAAGTAACA
    GTACCTGAAT CCATCATCGC TGACCTGGAG AGGACCGCAC ACTCAACCAA TGCAGAGCGG
    AAGAGGCCGG AGCAAATGTC TGAAGATGTA CACCGACAAT ATGTGCAACA ACTGCAGGAC
    ACTCTTCTAA CTGACGTTCA GAAGAACCTA GAAGACTTTA GGCACAAGCG TAGTATGGGT
    CTGACATTGG CTGAAGCTGA ACTGGCCCGT CTGGATACAG AGAGAATGAG AGATCGTGTG
    GCTTTGGAAA AAGAGCGATC CTGTGCAGAG CACATCCTTT CTAAGATAGA AGAAGTCCTA
    TTGACGTCAC AAGCCACAGA GGAAGATAAG TGTAGCACCA TGCAATTTGT GATACTATCG
    TATATGAAAC AGCTCGGTGT GAAAGTGAAG GAGTTGCGAG GGCTGGACCA CAAACCCAAG
    TTAATGAATC CATTCCACAA AATAAAGAAA AGTATGAAGC CTGAGAAGGA TGGAAGTGAG
    GAGAAAGCGA AGAAAACACG ATTTCCTAAT GTCTTTCCTC AGAGACGACC AAGCAAGATT
    GACGCTTTAT CACAGCCGAA GCAGCGTACG CCAAAGCCTC AGCTGCCTCT GGGAACTGGA
    GAGCAGGCAG ACGGGTCGTC TCTTCCTGTA CGGAGCAGTC AGTCCAGCGA GGGCTCAGAT
    GGCACCAATG CCCTGATCAC ATCACCGCCT TACAGCGCTC CAGCAGGACA GGGCACAGAC
    GCCATCAGCC GAGAGTCTGA GACTGGGGGA CTTCCCTCTT CTGCCCTGCC CAAATTGGGA
    GAGGGTACTG TCTCAGGGGA TTTGCAGGAC TCCTCATCTA CAAACACTCA CTTTGACTTC
    AGCAATTGCA TGCTGGAGCA TTTACAGGAG GAAGAAGCTG AGACTGAAAA GACGCAGGAC
    TCTGGCACCC CCAGGCCTGA CAGGAGCAGG ATGGATCCGC TGGGCTCTGG AGAGGTGCAG
    AGTGAAGATG ATCAGTGTGT TGAAGCCGAA CTCGAGCCTC CAAACTGGCA GACACTAGTG
    AGCAGAGACG TCCTGTCCAC ACTCACGCCT CAGGAAATTA AGAGACAAGA AGTCATCAAT
    GAGCTGTTCT ACACGGAGCG AGCACACGTT CGAATGATGA AGGTTCTGGA GAATTTATTT
    TACCAGCCGC TGATCAGAGA CGCCATCCTT CCTCCTGCAG ACATCAAAAA CATCTTCAGC
    AACCTGGAGG AGATCGTTCA GCTGCACTTG TCTTTAACCG AACAAATGGC CGCCGTACGG
    AAGAAGAACG AGACCGCTCT CATTGACTCA ATAGGAGATG ATCTGCTTTC CTGGTTCAGT
    GAAGAAGAGG AGGATAAGAT CAAGAGAGCG GTGGGCACCT ACTGCAGTAA CCAGCCCTTC
    GCCCTGGAGC TCATCAAGAG CAAACAGAAG AAAGACCAGC GCTTCAATGC CTTCATACAG
    GAGACAGAGA GCAACCGTCA TTGTCGCAGG CTGCAGTTGA AAGACATTAT TCCTGTGGAG
    ACGTTACGAC TCACAAAGTA TCCTCTTCTG CTGGAGAACA TCGCCAAATA CACAGAGGAC
    TCTGAGGAGA GAAGTAAAGT GAAACGGGCT GGCGAATGCT GTAGAAAGAT CCTCACTCAC
    GTCAACCAGG AGGTGAAGGA AGCGGAAAAC AAGCAGAGGC TGGAGGACTA CCAGAGAAGA
    CTGGACCTGT CCTCACTGAA GCAGAGCGAA AACCCCATGA TCGCAGAGTT CAAGAGCTTG
    GACTTGACCA AGAGGAAGAT GGTGCACGAG GGGCCGCTGT CCTGGAAGGT CAACAAAGAC
    AAAACCATTG AGCTGTACAC GTTGCTGCTG GAGGATATAT TGGTGCTACT TCAAAGGCAG
    GATGAGAAGC TGATCTTAAA ATGCCACAGT AAGAATCTGG CGGGCACCGC TGAGACCAAA
    CACATCTTCA GCCCCATCAT CAAGCTCAGC ACTGTGATGG TGCGCTCCGT TGCCACCGAC
    AACAGGTCGT TCTTTGTGCT GTCGATGTCT GATAATGGAG CCCAGATCTA TGAGCTGATG
    GCCCAGACCG TATCAGAGCA AAAGACGTGG CAGTGTCTGA TCACACAAAG AGCAGACTGC
    ATGAAGACCA AACTCCACAA CATCATTCCA TTACCTCAGA CCGATGGGGA ACGTGAAGAG
    GTGGAGATGA TCAACTCTGG TGTTCAGAAG ATAAGCAAAG ACACTGAACC AATTACTTTA
    GCGAGCATCC AGTCTCCAGT AGAAAAAGAC GACACTGAGG TCATCCCAAC CCCACTACCC
    AGCACCAACC CCTTTGAGAC CAAATCTGAT GAGGAGGCTG AAGAAGAGGA GGAAAACTCT
    CTACAGGACC AGGCTGAAGA CGATGAAGCG TTTGTTGTGG CAGATCTGAG CGATCGACTG
    ACGTTCCTGA AACAGCGCTC CAGACTCGGC ATCGCCATCG ATGAGGACGA GGCCGAAGTC
    TTCGAGCTGC AGCCGCTCAT AGCTGATGAA GCTCTCAGGA CATTGGCGAC ATTGAGGCAA
    CTCCTGATCA ACCACTTTTC CGGTCAGGAG GACACAGACA GAGAGAACAA GAGTGAAGAT
    CAACAGGAGC ATATAGAGAG GACGTGCCCT CAGCAGGGCT CAGACGACAG CGCTGCCATC
    CAGTCTCCGC TTGAGAACGG CTCAGACAGA GGAGAGAATC CACAGGAACT ACCATCCGGG
    GACACCGGCT TCTTTGAGAC GTCTGAAGAT TGTGTGTCTG CAGGCAGTTA TATGGTGCTG
    GAGGTGTTTG CGGGCTCAGG AGAGAGCAGC ACAGATGACG ATGCGCAGGG CTGCAGTGTG
    GAGCCTGTGG CTGGTGGAGA TTCAGGCATC GACTTGAAGA AGCTCCTGTC TTCATCTTCT
    CAAAGCACTG GAGGACCCAA CATCAACAGA CAGATCATGA CACACTTACG TCTACTACAG
    GGCAACCTGC AGCAGCTGAA GGAGACCGAA ATCAAGTATA ACCAGCTACG CCAAAGGCTA
    TCAGAGGAAA CAGCTACTGA CACAGAGGAA AACAAAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.