Arhgef12 cDNA ORF clone, Mus musculus(house mouse)

The following Arhgef12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgef12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu70444 XM_011242598.3
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu82922 XM_030244571.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu82922 XM_017313567.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu90246 XM_030244572.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu90246 XM_030244573.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu70443 XM_011242597.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu82920 XM_017313565.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu82921 XM_017313566.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu21950 NM_027144.2
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $909.30
$1299.00
OMu42386 NM_001359232.1
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu42386 XM_006510586.3
Latest version!
Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OMu82921 XM_017313566.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu42386 XM_006510586.2 Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu70443 XM_011242597.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu82922 XM_017313567.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu82920 XM_017313565.1 Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu70444 XM_011242598.2 Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu70444
    Clone ID Related Accession (Same CDS sequence) XM_011242598.2 , XM_011242598.3
    Accession Version XM_011242598.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC 
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011240900.1
    CDS1673..5803
    Translation

    Target ORF information:

    RefSeq Version XM_011242598.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011242598.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC 
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82922
    Clone ID Related Accession (Same CDS sequence) XM_017313567.2 , XM_030244571.1 , XM_017313567.1
    Accession Version XM_017313567.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313567.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT CAGGAGCCGC AGTCGCTGGT CGGCAGTCCC
    TCCACCCGGG GCGCCCCTCA CATCATTGGA GCAGAGGATG ATGATTTTGG TACTGAGCAT
    GAGCAGATCA ATGGACAGTG CAGCTGTTTC CAGAGCATTG AATTGCTGAA GTCTCGCCCT
    GCTCACTTGG CTGTTTTCTT ACACCATGTA GTCTCACAGT TTGACCCTGC AACATTGCTC
    TGTTATCTCT ATTCAGACCT GTATAAGCAG ACAAACTCCA AGGAGACTCG GCGTGTCTTC
    CTGGAGTTTC ATCAGTTCTT CTTGGACCGG TCTGCACACC TGAAGGTTCC AGTTCCTGAG
    GAAATCTCCG TGGATCTAGA AAAGAGAAGA CCTGAGCTTA TTCCTGAGGA TCTGCATCGC
    CTCTACATTC AGACAATGCA GGAGAGAGTC CACCCTGAAG TTCAGAGGCA CTTGGAGGAT
    TTCCGACAGA AACGCAGCAT GGGGCTGACC TTGGCTGAGA GTGAGCTGAC TAAACTTGAT
    GCAGAGAGAG ACAAGGACCG GGGAACTCTG GAGAAGGAGC GAGCATGTGC GGAACAGATC
    GTTACCAAAA TTGAAGAAGT GTTGATGACT GCTCAGGCTG TAGAAGAAGA AAGGAGTTCC
    ACAATGCAGT ATGTAATTCT CATGTATATG AAGTATTTGG GAGTAAAAGT GAAAGAACCT
    CGAAACTTGG AGCACAAGCG GGGTCGGATC GGCTTCCTTC CAAAGATCAA GCAAAGCATG
    AAGAAAGATA GAGAGGGCGA AGAAAAAGGG AAGCGAAGAG GATTCCCCAG CATTTTGGGA
    CCCCCAAGAA GACCAAGCCG CCACGACAAC AGTGCAATTG GCAGGGCCAT GGAGATACAG
    AAGTCACGCC ACCCGAAGCA CTTATCCACT CCCTCGTCTG TGAGTCCTGA GCCTCAGGAC
    CCTGCCAAGT TGCGCCAGAG TGGAGTAGCC AATGAAGGAA CAGACACTGG ATACCTGCCC
    GCCAGTTCCA TGTCCTCTGC CACCTCAGGG ACTGCTCTTT CCCAAGAGGG AGGGAGAGAG
    AATGATACAG GAACAAAACA AGTGGGAGAA GCATCGGCGC CTGGAGACTG CTTGGACAGC
    ACACCTCGAG TCCCCACTAC TGTTTTCGAC TTCCCTCCTC CTCTATTAGA TCAAGTGCAG
    GAGGAGGAGT GTGAAGTAGA GAGGGTGGCA GAACATGGGA CCCCAAAACC CTTTCGGAAG
    TTTGACAGCA TCGCCTTTGG AGAAAGTCAG AGTGAGGATG AGCAGTTTGA AAATGACCTG
    GAGACAGATC CACCCAACTG GCAGCAGCTT GTTAGCAGAG AAGTGTTACT GGGACTGAAA
    CCTTCTGAGA TCAAAAGACA GGAAGTGATT AATGAATTGT TCTACACTGA GAGAGCTCAT
    GTCCGGACAC TGAAGGTTCT TGACCAAGTA TTCTATCAGC GAGTGTCCAG AGAAGGGATT
    CTATCACCTT CAGAACTACG CAAGATTTTT TCAAACTTGG AAGATATCCT TCAACTTCAT
    GTTGGGTTGA ATGAGCAAAT GAAAGCTGTC CGAAAAAGGA ATGAAACTTC TGTTATTGAT
    CATATTGGGG AAGATCTGCT CATCTGGTTT AGTGGACCAG GAGAGGAGAA GCTGAAACAT
    GCTGCTGCCA CATTCTGCAG TAACCAACCT TTCGCCCTGG AAATGATCAA GTCTCGCCAG
    AAAAAGGATT CTCGATTCCA TACTTTTGTG CAAGACGCTG AGAGTAACCC CCTGTGCCGT
    CGTCTTCAGC TGAAGGACAT CATTCCCACC CAAATGCAGA GACTCACTAA GTACCCGCTT
    CTTTTGGATA ACATTGCCAA GTACACAGAA TGGCCTCCAG AAAGGGAAAA GGTGAAGAAG
    GCTGCAGACC ATTGTCGTCA GATCTTAAAT TATGTAAATC AGGCTGTCAG GGAGGCAGAA
    AACAAGCAGC GCTTAGAAGA CTATCAGCGT CGCCTTGACA CCTCCAATCT CAAGTTGTCC
    GAGTACCCAA ATGTTGACGA GCTCAGGAAT TTGGATTTAA CAAAAAGAAA GATGATTCAT
    GAAGGGCCGT TGGTCTGGAA GGTGAATAGA GATAAAAGCA TCGATCTTTA TACACTGCTG
    CTGGAAGACA TCCTTGTGCT GCTACAGAAG CAGGATGACA GGCTGGTGCT AAGGTGCCAC
    AGCAAGATTC TGGCATCCAC AGCCGACAGC AAACACACCT TCAGCCCTGT CATTAAGCTG
    AGCACGGTGC TGGTTCGGCA GGTGGCGACA GATAACAAAG CTTTATTCGT CATCTCCATG
    TCAGATAATG GAGCCCAAAT TTATGAATTG GTAGCACAGA CGGTTTCTGA AAAGACAGTC
    TGGCAAGACC TCATCTGTCG AATGGCTGCG TCAGTGAAGG AGCAGTCTAC AAAGCCGATT
    CCCCTCCCAC AGCCGCCACC TTGCGAAGGA GACAATGATG AAGAAGAACC AGCCAAGTTA
    AAAGTGGAGC ACCATGATCT TTCAGTCGCT GGTCTGCAAA GTCCAGACAG AGTTTTGGGA
    TTAGAATCTC CCTTAATTTC GTCAAAGCCT CAGTCTCATT CACTGAATAC CCCTGGCAAA
    TCAGCGGCAG AACATCTCTT TGTGACCGCA ACACAGTTTG CAAAGGAACA GCATGCAAAT
    GGGGCACTCA AGGAAGGTGA TGGCGGGTAT CCAGTCACAA TCCCAGGTCC ACACTTGCCT
    GTGTCAGAGG AGCGGTGGGC ATTGGACGCA CTGAGAAATT TGGGCTTGTT GAAGCAGTTG
    CTGGTACAGC AGTTAGGTCT CACCGAGAAG AGTACTCAGG AAGACTGGCA GTCCTTCTCA
    CGATACGGGC CAGCTTCTGA AGAGGTACAG GCAGACAGTG GCATCCGGGA CTTGGAAAAT
    GTTAAGGCTT GTCATGCCAG GGAAGGACAG ATGTCTTTTA AAACTGGAAC TGGTGATATT
    GCAACTTGTG ACAGTCCACG GACTTCAACT GAGTCTTGTG CTGCACAGGA TTCAGTGATA
    CTGGCATCCC AAGACAGCCA GGCAAGTAAC GTTTTAGTAA TGGACCACAT GATCCTGACC
    CCAGAGATGC CTCCTGCAGA GCCAGAAGGG GGTCTGGATG AGAGTGGAGA GCACTTTTTT
    GATGCCCGTG AAGCACATAG TGATGATAAT CCATCAGAAG GTGATGGAGC AGTTAAAAAG
    GAAGAGAAGG ATGTTAATTT ACGCATCTCA GGAAACTGTT TGATCCTTGA TGGCTATGAT
    GCAGTTCAGG AGAGCTCCAC AGATGAGGAG GTTGCCTCCT CGTTCCCCCT GCAGCCTGTG
    ACGGGCATCC CCGCTGTGGA CTCCAGCCAC CAGCAGCAAC ATTCCCCTCA GAATGTGCAT
    CCCGAGGGGC CGGTTTCACC ATTCACCCCA GAATTCCTGG TCCAGCGGCA CTGGCGAGCT
    ATGGAGGATA CCTGTTTTGA GATCCAGAGT CCCTCATGTA CAGATTCACA AAGCCAAATC
    CTGGAGTACA TTCATAAAAT AGAGGCTGAC CTGGAACACT TAAAGAAGGT GGAGGAAAGT
    TACGCTCTTC TTTGCCAAAG GCTGGCTGGC TCAGCCCTCC CCGACAAGCT CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169056.1
    CDS1474..5799
    Translation

    Target ORF information:

    RefSeq Version XM_017313567.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313567.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT CAGGAGCCGC AGTCGCTGGT CGGCAGTCCC
    TCCACCCGGG GCGCCCCTCA CATCATTGGA GCAGAGGATG ATGATTTTGG TACTGAGCAT
    GAGCAGATCA ATGGACAGTG CAGCTGTTTC CAGAGCATTG AATTGCTGAA GTCTCGCCCT
    GCTCACTTGG CTGTTTTCTT ACACCATGTA GTCTCACAGT TTGACCCTGC AACATTGCTC
    TGTTATCTCT ATTCAGACCT GTATAAGCAG ACAAACTCCA AGGAGACTCG GCGTGTCTTC
    CTGGAGTTTC ATCAGTTCTT CTTGGACCGG TCTGCACACC TGAAGGTTCC AGTTCCTGAG
    GAAATCTCCG TGGATCTAGA AAAGAGAAGA CCTGAGCTTA TTCCTGAGGA TCTGCATCGC
    CTCTACATTC AGACAATGCA GGAGAGAGTC CACCCTGAAG TTCAGAGGCA CTTGGAGGAT
    TTCCGACAGA AACGCAGCAT GGGGCTGACC TTGGCTGAGA GTGAGCTGAC TAAACTTGAT
    GCAGAGAGAG ACAAGGACCG GGGAACTCTG GAGAAGGAGC GAGCATGTGC GGAACAGATC
    GTTACCAAAA TTGAAGAAGT GTTGATGACT GCTCAGGCTG TAGAAGAAGA AAGGAGTTCC
    ACAATGCAGT ATGTAATTCT CATGTATATG AAGTATTTGG GAGTAAAAGT GAAAGAACCT
    CGAAACTTGG AGCACAAGCG GGGTCGGATC GGCTTCCTTC CAAAGATCAA GCAAAGCATG
    AAGAAAGATA GAGAGGGCGA AGAAAAAGGG AAGCGAAGAG GATTCCCCAG CATTTTGGGA
    CCCCCAAGAA GACCAAGCCG CCACGACAAC AGTGCAATTG GCAGGGCCAT GGAGATACAG
    AAGTCACGCC ACCCGAAGCA CTTATCCACT CCCTCGTCTG TGAGTCCTGA GCCTCAGGAC
    CCTGCCAAGT TGCGCCAGAG TGGAGTAGCC AATGAAGGAA CAGACACTGG ATACCTGCCC
    GCCAGTTCCA TGTCCTCTGC CACCTCAGGG ACTGCTCTTT CCCAAGAGGG AGGGAGAGAG
    AATGATACAG GAACAAAACA AGTGGGAGAA GCATCGGCGC CTGGAGACTG CTTGGACAGC
    ACACCTCGAG TCCCCACTAC TGTTTTCGAC TTCCCTCCTC CTCTATTAGA TCAAGTGCAG
    GAGGAGGAGT GTGAAGTAGA GAGGGTGGCA GAACATGGGA CCCCAAAACC CTTTCGGAAG
    TTTGACAGCA TCGCCTTTGG AGAAAGTCAG AGTGAGGATG AGCAGTTTGA AAATGACCTG
    GAGACAGATC CACCCAACTG GCAGCAGCTT GTTAGCAGAG AAGTGTTACT GGGACTGAAA
    CCTTCTGAGA TCAAAAGACA GGAAGTGATT AATGAATTGT TCTACACTGA GAGAGCTCAT
    GTCCGGACAC TGAAGGTTCT TGACCAAGTA TTCTATCAGC GAGTGTCCAG AGAAGGGATT
    CTATCACCTT CAGAACTACG CAAGATTTTT TCAAACTTGG AAGATATCCT TCAACTTCAT
    GTTGGGTTGA ATGAGCAAAT GAAAGCTGTC CGAAAAAGGA ATGAAACTTC TGTTATTGAT
    CATATTGGGG AAGATCTGCT CATCTGGTTT AGTGGACCAG GAGAGGAGAA GCTGAAACAT
    GCTGCTGCCA CATTCTGCAG TAACCAACCT TTCGCCCTGG AAATGATCAA GTCTCGCCAG
    AAAAAGGATT CTCGATTCCA TACTTTTGTG CAAGACGCTG AGAGTAACCC CCTGTGCCGT
    CGTCTTCAGC TGAAGGACAT CATTCCCACC CAAATGCAGA GACTCACTAA GTACCCGCTT
    CTTTTGGATA ACATTGCCAA GTACACAGAA TGGCCTCCAG AAAGGGAAAA GGTGAAGAAG
    GCTGCAGACC ATTGTCGTCA GATCTTAAAT TATGTAAATC AGGCTGTCAG GGAGGCAGAA
    AACAAGCAGC GCTTAGAAGA CTATCAGCGT CGCCTTGACA CCTCCAATCT CAAGTTGTCC
    GAGTACCCAA ATGTTGACGA GCTCAGGAAT TTGGATTTAA CAAAAAGAAA GATGATTCAT
    GAAGGGCCGT TGGTCTGGAA GGTGAATAGA GATAAAAGCA TCGATCTTTA TACACTGCTG
    CTGGAAGACA TCCTTGTGCT GCTACAGAAG CAGGATGACA GGCTGGTGCT AAGGTGCCAC
    AGCAAGATTC TGGCATCCAC AGCCGACAGC AAACACACCT TCAGCCCTGT CATTAAGCTG
    AGCACGGTGC TGGTTCGGCA GGTGGCGACA GATAACAAAG CTTTATTCGT CATCTCCATG
    TCAGATAATG GAGCCCAAAT TTATGAATTG GTAGCACAGA CGGTTTCTGA AAAGACAGTC
    TGGCAAGACC TCATCTGTCG AATGGCTGCG TCAGTGAAGG AGCAGTCTAC AAAGCCGATT
    CCCCTCCCAC AGCCGCCACC TTGCGAAGGA GACAATGATG AAGAAGAACC AGCCAAGTTA
    AAAGTGGAGC ACCATGATCT TTCAGTCGCT GGTCTGCAAA GTCCAGACAG AGTTTTGGGA
    TTAGAATCTC CCTTAATTTC GTCAAAGCCT CAGTCTCATT CACTGAATAC CCCTGGCAAA
    TCAGCGGCAG AACATCTCTT TGTGACCGCA ACACAGTTTG CAAAGGAACA GCATGCAAAT
    GGGGCACTCA AGGAAGGTGA TGGCGGGTAT CCAGTCACAA TCCCAGGTCC ACACTTGCCT
    GTGTCAGAGG AGCGGTGGGC ATTGGACGCA CTGAGAAATT TGGGCTTGTT GAAGCAGTTG
    CTGGTACAGC AGTTAGGTCT CACCGAGAAG AGTACTCAGG AAGACTGGCA GTCCTTCTCA
    CGATACGGGC CAGCTTCTGA AGAGGTACAG GCAGACAGTG GCATCCGGGA CTTGGAAAAT
    GTTAAGGCTT GTCATGCCAG GGAAGGACAG ATGTCTTTTA AAACTGGAAC TGGTGATATT
    GCAACTTGTG ACAGTCCACG GACTTCAACT GAGTCTTGTG CTGCACAGGA TTCAGTGATA
    CTGGCATCCC AAGACAGCCA GGCAAGTAAC GTTTTAGTAA TGGACCACAT GATCCTGACC
    CCAGAGATGC CTCCTGCAGA GCCAGAAGGG GGTCTGGATG AGAGTGGAGA GCACTTTTTT
    GATGCCCGTG AAGCACATAG TGATGATAAT CCATCAGAAG GTGATGGAGC AGTTAAAAAG
    GAAGAGAAGG ATGTTAATTT ACGCATCTCA GGAAACTGTT TGATCCTTGA TGGCTATGAT
    GCAGTTCAGG AGAGCTCCAC AGATGAGGAG GTTGCCTCCT CGTTCCCCCT GCAGCCTGTG
    ACGGGCATCC CCGCTGTGGA CTCCAGCCAC CAGCAGCAAC ATTCCCCTCA GAATGTGCAT
    CCCGAGGGGC CGGTTTCACC ATTCACCCCA GAATTCCTGG TCCAGCGGCA CTGGCGAGCT
    ATGGAGGATA CCTGTTTTGA GATCCAGAGT CCCTCATGTA CAGATTCACA AAGCCAAATC
    CTGGAGTACA TTCATAAAAT AGAGGCTGAC CTGGAACACT TAAAGAAGGT GGAGGAAAGT
    TACGCTCTTC TTTGCCAAAG GCTGGCTGGC TCAGCCCTCC CCGACAAGCT CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82922
    Clone ID Related Accession (Same CDS sequence) XM_017313567.2 , XM_030244571.1 , XM_017313567.1
    Accession Version XM_030244571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT CAGGAGCCGC AGTCGCTGGT CGGCAGTCCC
    TCCACCCGGG GCGCCCCTCA CATCATTGGA GCAGAGGATG ATGATTTTGG TACTGAGCAT
    GAGCAGATCA ATGGACAGTG CAGCTGTTTC CAGAGCATTG AATTGCTGAA GTCTCGCCCT
    GCTCACTTGG CTGTTTTCTT ACACCATGTA GTCTCACAGT TTGACCCTGC AACATTGCTC
    TGTTATCTCT ATTCAGACCT GTATAAGCAG ACAAACTCCA AGGAGACTCG GCGTGTCTTC
    CTGGAGTTTC ATCAGTTCTT CTTGGACCGG TCTGCACACC TGAAGGTTCC AGTTCCTGAG
    GAAATCTCCG TGGATCTAGA AAAGAGAAGA CCTGAGCTTA TTCCTGAGGA TCTGCATCGC
    CTCTACATTC AGACAATGCA GGAGAGAGTC CACCCTGAAG TTCAGAGGCA CTTGGAGGAT
    TTCCGACAGA AACGCAGCAT GGGGCTGACC TTGGCTGAGA GTGAGCTGAC TAAACTTGAT
    GCAGAGAGAG ACAAGGACCG GGGAACTCTG GAGAAGGAGC GAGCATGTGC GGAACAGATC
    GTTACCAAAA TTGAAGAAGT GTTGATGACT GCTCAGGCTG TAGAAGAAGA AAGGAGTTCC
    ACAATGCAGT ATGTAATTCT CATGTATATG AAGTATTTGG GAGTAAAAGT GAAAGAACCT
    CGAAACTTGG AGCACAAGCG GGGTCGGATC GGCTTCCTTC CAAAGATCAA GCAAAGCATG
    AAGAAAGATA GAGAGGGCGA AGAAAAAGGG AAGCGAAGAG GATTCCCCAG CATTTTGGGA
    CCCCCAAGAA GACCAAGCCG CCACGACAAC AGTGCAATTG GCAGGGCCAT GGAGATACAG
    AAGTCACGCC ACCCGAAGCA CTTATCCACT CCCTCGTCTG TGAGTCCTGA GCCTCAGGAC
    CCTGCCAAGT TGCGCCAGAG TGGAGTAGCC AATGAAGGAA CAGACACTGG ATACCTGCCC
    GCCAGTTCCA TGTCCTCTGC CACCTCAGGG ACTGCTCTTT CCCAAGAGGG AGGGAGAGAG
    AATGATACAG GAACAAAACA AGTGGGAGAA GCATCGGCGC CTGGAGACTG CTTGGACAGC
    ACACCTCGAG TCCCCACTAC TGTTTTCGAC TTCCCTCCTC CTCTATTAGA TCAAGTGCAG
    GAGGAGGAGT GTGAAGTAGA GAGGGTGGCA GAACATGGGA CCCCAAAACC CTTTCGGAAG
    TTTGACAGCA TCGCCTTTGG AGAAAGTCAG AGTGAGGATG AGCAGTTTGA AAATGACCTG
    GAGACAGATC CACCCAACTG GCAGCAGCTT GTTAGCAGAG AAGTGTTACT GGGACTGAAA
    CCTTCTGAGA TCAAAAGACA GGAAGTGATT AATGAATTGT TCTACACTGA GAGAGCTCAT
    GTCCGGACAC TGAAGGTTCT TGACCAAGTA TTCTATCAGC GAGTGTCCAG AGAAGGGATT
    CTATCACCTT CAGAACTACG CAAGATTTTT TCAAACTTGG AAGATATCCT TCAACTTCAT
    GTTGGGTTGA ATGAGCAAAT GAAAGCTGTC CGAAAAAGGA ATGAAACTTC TGTTATTGAT
    CATATTGGGG AAGATCTGCT CATCTGGTTT AGTGGACCAG GAGAGGAGAA GCTGAAACAT
    GCTGCTGCCA CATTCTGCAG TAACCAACCT TTCGCCCTGG AAATGATCAA GTCTCGCCAG
    AAAAAGGATT CTCGATTCCA TACTTTTGTG CAAGACGCTG AGAGTAACCC CCTGTGCCGT
    CGTCTTCAGC TGAAGGACAT CATTCCCACC CAAATGCAGA GACTCACTAA GTACCCGCTT
    CTTTTGGATA ACATTGCCAA GTACACAGAA TGGCCTCCAG AAAGGGAAAA GGTGAAGAAG
    GCTGCAGACC ATTGTCGTCA GATCTTAAAT TATGTAAATC AGGCTGTCAG GGAGGCAGAA
    AACAAGCAGC GCTTAGAAGA CTATCAGCGT CGCCTTGACA CCTCCAATCT CAAGTTGTCC
    GAGTACCCAA ATGTTGACGA GCTCAGGAAT TTGGATTTAA CAAAAAGAAA GATGATTCAT
    GAAGGGCCGT TGGTCTGGAA GGTGAATAGA GATAAAAGCA TCGATCTTTA TACACTGCTG
    CTGGAAGACA TCCTTGTGCT GCTACAGAAG CAGGATGACA GGCTGGTGCT AAGGTGCCAC
    AGCAAGATTC TGGCATCCAC AGCCGACAGC AAACACACCT TCAGCCCTGT CATTAAGCTG
    AGCACGGTGC TGGTTCGGCA GGTGGCGACA GATAACAAAG CTTTATTCGT CATCTCCATG
    TCAGATAATG GAGCCCAAAT TTATGAATTG GTAGCACAGA CGGTTTCTGA AAAGACAGTC
    TGGCAAGACC TCATCTGTCG AATGGCTGCG TCAGTGAAGG AGCAGTCTAC AAAGCCGATT
    CCCCTCCCAC AGCCGCCACC TTGCGAAGGA GACAATGATG AAGAAGAACC AGCCAAGTTA
    AAAGTGGAGC ACCATGATCT TTCAGTCGCT GGTCTGCAAA GTCCAGACAG AGTTTTGGGA
    TTAGAATCTC CCTTAATTTC GTCAAAGCCT CAGTCTCATT CACTGAATAC CCCTGGCAAA
    TCAGCGGCAG AACATCTCTT TGTGACCGCA ACACAGTTTG CAAAGGAACA GCATGCAAAT
    GGGGCACTCA AGGAAGGTGA TGGCGGGTAT CCAGTCACAA TCCCAGGTCC ACACTTGCCT
    GTGTCAGAGG AGCGGTGGGC ATTGGACGCA CTGAGAAATT TGGGCTTGTT GAAGCAGTTG
    CTGGTACAGC AGTTAGGTCT CACCGAGAAG AGTACTCAGG AAGACTGGCA GTCCTTCTCA
    CGATACGGGC CAGCTTCTGA AGAGGTACAG GCAGACAGTG GCATCCGGGA CTTGGAAAAT
    GTTAAGGCTT GTCATGCCAG GGAAGGACAG ATGTCTTTTA AAACTGGAAC TGGTGATATT
    GCAACTTGTG ACAGTCCACG GACTTCAACT GAGTCTTGTG CTGCACAGGA TTCAGTGATA
    CTGGCATCCC AAGACAGCCA GGCAAGTAAC GTTTTAGTAA TGGACCACAT GATCCTGACC
    CCAGAGATGC CTCCTGCAGA GCCAGAAGGG GGTCTGGATG AGAGTGGAGA GCACTTTTTT
    GATGCCCGTG AAGCACATAG TGATGATAAT CCATCAGAAG GTGATGGAGC AGTTAAAAAG
    GAAGAGAAGG ATGTTAATTT ACGCATCTCA GGAAACTGTT TGATCCTTGA TGGCTATGAT
    GCAGTTCAGG AGAGCTCCAC AGATGAGGAG GTTGCCTCCT CGTTCCCCCT GCAGCCTGTG
    ACGGGCATCC CCGCTGTGGA CTCCAGCCAC CAGCAGCAAC ATTCCCCTCA GAATGTGCAT
    CCCGAGGGGC CGGTTTCACC ATTCACCCCA GAATTCCTGG TCCAGCGGCA CTGGCGAGCT
    ATGGAGGATA CCTGTTTTGA GATCCAGAGT CCCTCATGTA CAGATTCACA AAGCCAAATC
    CTGGAGTACA TTCATAAAAT AGAGGCTGAC CTGGAACACT TAAAGAAGGT GGAGGAAAGT
    TACGCTCTTC TTTGCCAAAG GCTGGCTGGC TCAGCCCTCC CCGACAAGCT CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100431.1
    CDS1531..5856
    Translation

    Target ORF information:

    RefSeq Version XM_030244571.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030244571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT CAGGAGCCGC AGTCGCTGGT CGGCAGTCCC
    TCCACCCGGG GCGCCCCTCA CATCATTGGA GCAGAGGATG ATGATTTTGG TACTGAGCAT
    GAGCAGATCA ATGGACAGTG CAGCTGTTTC CAGAGCATTG AATTGCTGAA GTCTCGCCCT
    GCTCACTTGG CTGTTTTCTT ACACCATGTA GTCTCACAGT TTGACCCTGC AACATTGCTC
    TGTTATCTCT ATTCAGACCT GTATAAGCAG ACAAACTCCA AGGAGACTCG GCGTGTCTTC
    CTGGAGTTTC ATCAGTTCTT CTTGGACCGG TCTGCACACC TGAAGGTTCC AGTTCCTGAG
    GAAATCTCCG TGGATCTAGA AAAGAGAAGA CCTGAGCTTA TTCCTGAGGA TCTGCATCGC
    CTCTACATTC AGACAATGCA GGAGAGAGTC CACCCTGAAG TTCAGAGGCA CTTGGAGGAT
    TTCCGACAGA AACGCAGCAT GGGGCTGACC TTGGCTGAGA GTGAGCTGAC TAAACTTGAT
    GCAGAGAGAG ACAAGGACCG GGGAACTCTG GAGAAGGAGC GAGCATGTGC GGAACAGATC
    GTTACCAAAA TTGAAGAAGT GTTGATGACT GCTCAGGCTG TAGAAGAAGA AAGGAGTTCC
    ACAATGCAGT ATGTAATTCT CATGTATATG AAGTATTTGG GAGTAAAAGT GAAAGAACCT
    CGAAACTTGG AGCACAAGCG GGGTCGGATC GGCTTCCTTC CAAAGATCAA GCAAAGCATG
    AAGAAAGATA GAGAGGGCGA AGAAAAAGGG AAGCGAAGAG GATTCCCCAG CATTTTGGGA
    CCCCCAAGAA GACCAAGCCG CCACGACAAC AGTGCAATTG GCAGGGCCAT GGAGATACAG
    AAGTCACGCC ACCCGAAGCA CTTATCCACT CCCTCGTCTG TGAGTCCTGA GCCTCAGGAC
    CCTGCCAAGT TGCGCCAGAG TGGAGTAGCC AATGAAGGAA CAGACACTGG ATACCTGCCC
    GCCAGTTCCA TGTCCTCTGC CACCTCAGGG ACTGCTCTTT CCCAAGAGGG AGGGAGAGAG
    AATGATACAG GAACAAAACA AGTGGGAGAA GCATCGGCGC CTGGAGACTG CTTGGACAGC
    ACACCTCGAG TCCCCACTAC TGTTTTCGAC TTCCCTCCTC CTCTATTAGA TCAAGTGCAG
    GAGGAGGAGT GTGAAGTAGA GAGGGTGGCA GAACATGGGA CCCCAAAACC CTTTCGGAAG
    TTTGACAGCA TCGCCTTTGG AGAAAGTCAG AGTGAGGATG AGCAGTTTGA AAATGACCTG
    GAGACAGATC CACCCAACTG GCAGCAGCTT GTTAGCAGAG AAGTGTTACT GGGACTGAAA
    CCTTCTGAGA TCAAAAGACA GGAAGTGATT AATGAATTGT TCTACACTGA GAGAGCTCAT
    GTCCGGACAC TGAAGGTTCT TGACCAAGTA TTCTATCAGC GAGTGTCCAG AGAAGGGATT
    CTATCACCTT CAGAACTACG CAAGATTTTT TCAAACTTGG AAGATATCCT TCAACTTCAT
    GTTGGGTTGA ATGAGCAAAT GAAAGCTGTC CGAAAAAGGA ATGAAACTTC TGTTATTGAT
    CATATTGGGG AAGATCTGCT CATCTGGTTT AGTGGACCAG GAGAGGAGAA GCTGAAACAT
    GCTGCTGCCA CATTCTGCAG TAACCAACCT TTCGCCCTGG AAATGATCAA GTCTCGCCAG
    AAAAAGGATT CTCGATTCCA TACTTTTGTG CAAGACGCTG AGAGTAACCC CCTGTGCCGT
    CGTCTTCAGC TGAAGGACAT CATTCCCACC CAAATGCAGA GACTCACTAA GTACCCGCTT
    CTTTTGGATA ACATTGCCAA GTACACAGAA TGGCCTCCAG AAAGGGAAAA GGTGAAGAAG
    GCTGCAGACC ATTGTCGTCA GATCTTAAAT TATGTAAATC AGGCTGTCAG GGAGGCAGAA
    AACAAGCAGC GCTTAGAAGA CTATCAGCGT CGCCTTGACA CCTCCAATCT CAAGTTGTCC
    GAGTACCCAA ATGTTGACGA GCTCAGGAAT TTGGATTTAA CAAAAAGAAA GATGATTCAT
    GAAGGGCCGT TGGTCTGGAA GGTGAATAGA GATAAAAGCA TCGATCTTTA TACACTGCTG
    CTGGAAGACA TCCTTGTGCT GCTACAGAAG CAGGATGACA GGCTGGTGCT AAGGTGCCAC
    AGCAAGATTC TGGCATCCAC AGCCGACAGC AAACACACCT TCAGCCCTGT CATTAAGCTG
    AGCACGGTGC TGGTTCGGCA GGTGGCGACA GATAACAAAG CTTTATTCGT CATCTCCATG
    TCAGATAATG GAGCCCAAAT TTATGAATTG GTAGCACAGA CGGTTTCTGA AAAGACAGTC
    TGGCAAGACC TCATCTGTCG AATGGCTGCG TCAGTGAAGG AGCAGTCTAC AAAGCCGATT
    CCCCTCCCAC AGCCGCCACC TTGCGAAGGA GACAATGATG AAGAAGAACC AGCCAAGTTA
    AAAGTGGAGC ACCATGATCT TTCAGTCGCT GGTCTGCAAA GTCCAGACAG AGTTTTGGGA
    TTAGAATCTC CCTTAATTTC GTCAAAGCCT CAGTCTCATT CACTGAATAC CCCTGGCAAA
    TCAGCGGCAG AACATCTCTT TGTGACCGCA ACACAGTTTG CAAAGGAACA GCATGCAAAT
    GGGGCACTCA AGGAAGGTGA TGGCGGGTAT CCAGTCACAA TCCCAGGTCC ACACTTGCCT
    GTGTCAGAGG AGCGGTGGGC ATTGGACGCA CTGAGAAATT TGGGCTTGTT GAAGCAGTTG
    CTGGTACAGC AGTTAGGTCT CACCGAGAAG AGTACTCAGG AAGACTGGCA GTCCTTCTCA
    CGATACGGGC CAGCTTCTGA AGAGGTACAG GCAGACAGTG GCATCCGGGA CTTGGAAAAT
    GTTAAGGCTT GTCATGCCAG GGAAGGACAG ATGTCTTTTA AAACTGGAAC TGGTGATATT
    GCAACTTGTG ACAGTCCACG GACTTCAACT GAGTCTTGTG CTGCACAGGA TTCAGTGATA
    CTGGCATCCC AAGACAGCCA GGCAAGTAAC GTTTTAGTAA TGGACCACAT GATCCTGACC
    CCAGAGATGC CTCCTGCAGA GCCAGAAGGG GGTCTGGATG AGAGTGGAGA GCACTTTTTT
    GATGCCCGTG AAGCACATAG TGATGATAAT CCATCAGAAG GTGATGGAGC AGTTAAAAAG
    GAAGAGAAGG ATGTTAATTT ACGCATCTCA GGAAACTGTT TGATCCTTGA TGGCTATGAT
    GCAGTTCAGG AGAGCTCCAC AGATGAGGAG GTTGCCTCCT CGTTCCCCCT GCAGCCTGTG
    ACGGGCATCC CCGCTGTGGA CTCCAGCCAC CAGCAGCAAC ATTCCCCTCA GAATGTGCAT
    CCCGAGGGGC CGGTTTCACC ATTCACCCCA GAATTCCTGG TCCAGCGGCA CTGGCGAGCT
    ATGGAGGATA CCTGTTTTGA GATCCAGAGT CCCTCATGTA CAGATTCACA AAGCCAAATC
    CTGGAGTACA TTCATAAAAT AGAGGCTGAC CTGGAACACT TAAAGAAGGT GGAGGAAAGT
    TACGCTCTTC TTTGCCAAAG GCTGGCTGGC TCAGCCCTCC CCGACAAGCT CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82922
    Clone ID Related Accession (Same CDS sequence) XM_017313567.2 , XM_030244571.1 , XM_017313567.1
    Accession Version XM_017313567.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4326bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT CAGGAGCCGC AGTCGCTGGT CGGCAGTCCC
    TCCACCCGGG GCGCCCCTCA CATCATTGGA GCAGAGGATG ATGATTTTGG TACTGAGCAT
    GAGCAGATCA ATGGACAGTG CAGCTGTTTC CAGAGCATTG AATTGCTGAA GTCTCGCCCT
    GCTCACTTGG CTGTTTTCTT ACACCATGTA GTCTCACAGT TTGACCCTGC AACATTGCTC
    TGTTATCTCT ATTCAGACCT GTATAAGCAG ACAAACTCCA AGGAGACTCG GCGTGTCTTC
    CTGGAGTTTC ATCAGTTCTT CTTGGACCGG TCTGCACACC TGAAGGTTCC AGTTCCTGAG
    GAAATCTCCG TGGATCTAGA AAAGAGAAGA CCTGAGCTTA TTCCTGAGGA TCTGCATCGC
    CTCTACATTC AGACAATGCA GGAGAGAGTC CACCCTGAAG TTCAGAGGCA CTTGGAGGAT
    TTCCGACAGA AACGCAGCAT GGGGCTGACC TTGGCTGAGA GTGAGCTGAC TAAACTTGAT
    GCAGAGAGAG ACAAGGACCG GGGAACTCTG GAGAAGGAGC GAGCATGTGC GGAACAGATC
    GTTACCAAAA TTGAAGAAGT GTTGATGACT GCTCAGGCTG TAGAAGAAGA AAGGAGTTCC
    ACAATGCAGT ATGTAATTCT CATGTATATG AAGTATTTGG GAGTAAAAGT GAAAGAACCT
    CGAAACTTGG AGCACAAGCG GGGTCGGATC GGCTTCCTTC CAAAGATCAA GCAAAGCATG
    AAGAAAGATA GAGAGGGCGA AGAAAAAGGG AAGCGAAGAG GATTCCCCAG CATTTTGGGA
    CCCCCAAGAA GACCAAGCCG CCACGACAAC AGTGCAATTG GCAGGGCCAT GGAGATACAG
    AAGTCACGCC ACCCGAAGCA CTTATCCACT CCCTCGTCTG TGAGTCCTGA GCCTCAGGAC
    CCTGCCAAGT TGCGCCAGAG TGGAGTAGCC AATGAAGGAA CAGACACTGG ATACCTGCCC
    GCCAGTTCCA TGTCCTCTGC CACCTCAGGG ACTGCTCTTT CCCAAGAGGG AGGGAGAGAG
    AATGATACAG GAACAAAACA AGTGGGAGAA GCATCGGCGC CTGGAGACTG CTTGGACAGC
    ACACCTCGAG TCCCCACTAC TGTTTTCGAC TTCCCTCCTC CTCTATTAGA TCAAGTGCAG
    GAGGAGGAGT GTGAAGTAGA GAGGGTGGCA GAACATGGGA CCCCAAAACC CTTTCGGAAG
    TTTGACAGCA TCGCCTTTGG AGAAAGTCAG AGTGAGGATG AGCAGTTTGA AAATGACCTG
    GAGACAGATC CACCCAACTG GCAGCAGCTT GTTAGCAGAG AAGTGTTACT GGGACTGAAA
    CCTTCTGAGA TCAAAAGACA GGAAGTGATT AATGAATTGT TCTACACTGA GAGAGCTCAT
    GTCCGGACAC TGAAGGTTCT TGACCAAGTA TTCTATCAGC GAGTGTCCAG AGAAGGGATT
    CTATCACCTT CAGAACTACG CAAGATTTTT TCAAACTTGG AAGATATCCT TCAACTTCAT
    GTTGGGTTGA ATGAGCAAAT GAAAGCTGTC CGAAAAAGGA ATGAAACTTC TGTTATTGAT
    CATATTGGGG AAGATCTGCT CATCTGGTTT AGTGGACCAG GAGAGGAGAA GCTGAAACAT
    GCTGCTGCCA CATTCTGCAG TAACCAACCT TTCGCCCTGG AAATGATCAA GTCTCGCCAG
    AAAAAGGATT CTCGATTCCA TACTTTTGTG CAAGACGCTG AGAGTAACCC CCTGTGCCGT
    CGTCTTCAGC TGAAGGACAT CATTCCCACC CAAATGCAGA GACTCACTAA GTACCCGCTT
    CTTTTGGATA ACATTGCCAA GTACACAGAA TGGCCTCCAG AAAGGGAAAA GGTGAAGAAG
    GCTGCAGACC ATTGTCGTCA GATCTTAAAT TATGTAAATC AGGCTGTCAG GGAGGCAGAA
    AACAAGCAGC GCTTAGAAGA CTATCAGCGT CGCCTTGACA CCTCCAATCT CAAGTTGTCC
    GAGTACCCAA ATGTTGACGA GCTCAGGAAT TTGGATTTAA CAAAAAGAAA GATGATTCAT
    GAAGGGCCGT TGGTCTGGAA GGTGAATAGA GATAAAAGCA TCGATCTTTA TACACTGCTG
    CTGGAAGACA TCCTTGTGCT GCTACAGAAG CAGGATGACA GGCTGGTGCT AAGGTGCCAC
    AGCAAGATTC TGGCATCCAC AGCCGACAGC AAACACACCT TCAGCCCTGT CATTAAGCTG
    AGCACGGTGC TGGTTCGGCA GGTGGCGACA GATAACAAAG CTTTATTCGT CATCTCCATG
    TCAGATAATG GAGCCCAAAT TTATGAATTG GTAGCACAGA CGGTTTCTGA AAAGACAGTC
    TGGCAAGACC TCATCTGTCG AATGGCTGCG TCAGTGAAGG AGCAGTCTAC AAAGCCGATT
    CCCCTCCCAC AGCCGCCACC TTGCGAAGGA GACAATGATG AAGAAGAACC AGCCAAGTTA
    AAAGTGGAGC ACCATGATCT TTCAGTCGCT GGTCTGCAAA GTCCAGACAG AGTTTTGGGA
    TTAGAATCTC CCTTAATTTC GTCAAAGCCT CAGTCTCATT CACTGAATAC CCCTGGCAAA
    TCAGCGGCAG AACATCTCTT TGTGACCGCA ACACAGTTTG CAAAGGAACA GCATGCAAAT
    GGGGCACTCA AGGAAGGTGA TGGCGGGTAT CCAGTCACAA TCCCAGGTCC ACACTTGCCT
    GTGTCAGAGG AGCGGTGGGC ATTGGACGCA CTGAGAAATT TGGGCTTGTT GAAGCAGTTG
    CTGGTACAGC AGTTAGGTCT CACCGAGAAG AGTACTCAGG AAGACTGGCA GTCCTTCTCA
    CGATACGGGC CAGCTTCTGA AGAGGTACAG GCAGACAGTG GCATCCGGGA CTTGGAAAAT
    GTTAAGGCTT GTCATGCCAG GGAAGGACAG ATGTCTTTTA AAACTGGAAC TGGTGATATT
    GCAACTTGTG ACAGTCCACG GACTTCAACT GAGTCTTGTG CTGCACAGGA TTCAGTGATA
    CTGGCATCCC AAGACAGCCA GGCAAGTAAC GTTTTAGTAA TGGACCACAT GATCCTGACC
    CCAGAGATGC CTCCTGCAGA GCCAGAAGGG GGTCTGGATG AGAGTGGAGA GCACTTTTTT
    GATGCCCGTG AAGCACATAG TGATGATAAT CCATCAGAAG GTGATGGAGC AGTTAAAAAG
    GAAGAGAAGG ATGTTAATTT ACGCATCTCA GGAAACTGTT TGATCCTTGA TGGCTATGAT
    GCAGTTCAGG AGAGCTCCAC AGATGAGGAG GTTGCCTCCT CGTTCCCCCT GCAGCCTGTG
    ACGGGCATCC CCGCTGTGGA CTCCAGCCAC CAGCAGCAAC ATTCCCCTCA GAATGTGCAT
    CCCGAGGGGC CGGTTTCACC ATTCACCCCA GAATTCCTGG TCCAGCGGCA CTGGCGAGCT
    ATGGAGGATA CCTGTTTTGA GATCCAGAGT CCCTCATGTA CAGATTCACA AAGCCAAATC
    CTGGAGTACA TTCATAAAAT AGAGGCTGAC CTGGAACACT TAAAGAAGGT GGAGGAAAGT
    TACGCTCTTC TTTGCCAAAG GCTGGCTGGC TCAGCCCTCC CCGACAAGCT CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169056.1
    CDS312..4637
    Translation

    Target ORF information:

    RefSeq Version XM_017313567.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT CAGGAGCCGC AGTCGCTGGT CGGCAGTCCC
    TCCACCCGGG GCGCCCCTCA CATCATTGGA GCAGAGGATG ATGATTTTGG TACTGAGCAT
    GAGCAGATCA ATGGACAGTG CAGCTGTTTC CAGAGCATTG AATTGCTGAA GTCTCGCCCT
    GCTCACTTGG CTGTTTTCTT ACACCATGTA GTCTCACAGT TTGACCCTGC AACATTGCTC
    TGTTATCTCT ATTCAGACCT GTATAAGCAG ACAAACTCCA AGGAGACTCG GCGTGTCTTC
    CTGGAGTTTC ATCAGTTCTT CTTGGACCGG TCTGCACACC TGAAGGTTCC AGTTCCTGAG
    GAAATCTCCG TGGATCTAGA AAAGAGAAGA CCTGAGCTTA TTCCTGAGGA TCTGCATCGC
    CTCTACATTC AGACAATGCA GGAGAGAGTC CACCCTGAAG TTCAGAGGCA CTTGGAGGAT
    TTCCGACAGA AACGCAGCAT GGGGCTGACC TTGGCTGAGA GTGAGCTGAC TAAACTTGAT
    GCAGAGAGAG ACAAGGACCG GGGAACTCTG GAGAAGGAGC GAGCATGTGC GGAACAGATC
    GTTACCAAAA TTGAAGAAGT GTTGATGACT GCTCAGGCTG TAGAAGAAGA AAGGAGTTCC
    ACAATGCAGT ATGTAATTCT CATGTATATG AAGTATTTGG GAGTAAAAGT GAAAGAACCT
    CGAAACTTGG AGCACAAGCG GGGTCGGATC GGCTTCCTTC CAAAGATCAA GCAAAGCATG
    AAGAAAGATA GAGAGGGCGA AGAAAAAGGG AAGCGAAGAG GATTCCCCAG CATTTTGGGA
    CCCCCAAGAA GACCAAGCCG CCACGACAAC AGTGCAATTG GCAGGGCCAT GGAGATACAG
    AAGTCACGCC ACCCGAAGCA CTTATCCACT CCCTCGTCTG TGAGTCCTGA GCCTCAGGAC
    CCTGCCAAGT TGCGCCAGAG TGGAGTAGCC AATGAAGGAA CAGACACTGG ATACCTGCCC
    GCCAGTTCCA TGTCCTCTGC CACCTCAGGG ACTGCTCTTT CCCAAGAGGG AGGGAGAGAG
    AATGATACAG GAACAAAACA AGTGGGAGAA GCATCGGCGC CTGGAGACTG CTTGGACAGC
    ACACCTCGAG TCCCCACTAC TGTTTTCGAC TTCCCTCCTC CTCTATTAGA TCAAGTGCAG
    GAGGAGGAGT GTGAAGTAGA GAGGGTGGCA GAACATGGGA CCCCAAAACC CTTTCGGAAG
    TTTGACAGCA TCGCCTTTGG AGAAAGTCAG AGTGAGGATG AGCAGTTTGA AAATGACCTG
    GAGACAGATC CACCCAACTG GCAGCAGCTT GTTAGCAGAG AAGTGTTACT GGGACTGAAA
    CCTTCTGAGA TCAAAAGACA GGAAGTGATT AATGAATTGT TCTACACTGA GAGAGCTCAT
    GTCCGGACAC TGAAGGTTCT TGACCAAGTA TTCTATCAGC GAGTGTCCAG AGAAGGGATT
    CTATCACCTT CAGAACTACG CAAGATTTTT TCAAACTTGG AAGATATCCT TCAACTTCAT
    GTTGGGTTGA ATGAGCAAAT GAAAGCTGTC CGAAAAAGGA ATGAAACTTC TGTTATTGAT
    CATATTGGGG AAGATCTGCT CATCTGGTTT AGTGGACCAG GAGAGGAGAA GCTGAAACAT
    GCTGCTGCCA CATTCTGCAG TAACCAACCT TTCGCCCTGG AAATGATCAA GTCTCGCCAG
    AAAAAGGATT CTCGATTCCA TACTTTTGTG CAAGACGCTG AGAGTAACCC CCTGTGCCGT
    CGTCTTCAGC TGAAGGACAT CATTCCCACC CAAATGCAGA GACTCACTAA GTACCCGCTT
    CTTTTGGATA ACATTGCCAA GTACACAGAA TGGCCTCCAG AAAGGGAAAA GGTGAAGAAG
    GCTGCAGACC ATTGTCGTCA GATCTTAAAT TATGTAAATC AGGCTGTCAG GGAGGCAGAA
    AACAAGCAGC GCTTAGAAGA CTATCAGCGT CGCCTTGACA CCTCCAATCT CAAGTTGTCC
    GAGTACCCAA ATGTTGACGA GCTCAGGAAT TTGGATTTAA CAAAAAGAAA GATGATTCAT
    GAAGGGCCGT TGGTCTGGAA GGTGAATAGA GATAAAAGCA TCGATCTTTA TACACTGCTG
    CTGGAAGACA TCCTTGTGCT GCTACAGAAG CAGGATGACA GGCTGGTGCT AAGGTGCCAC
    AGCAAGATTC TGGCATCCAC AGCCGACAGC AAACACACCT TCAGCCCTGT CATTAAGCTG
    AGCACGGTGC TGGTTCGGCA GGTGGCGACA GATAACAAAG CTTTATTCGT CATCTCCATG
    TCAGATAATG GAGCCCAAAT TTATGAATTG GTAGCACAGA CGGTTTCTGA AAAGACAGTC
    TGGCAAGACC TCATCTGTCG AATGGCTGCG TCAGTGAAGG AGCAGTCTAC AAAGCCGATT
    CCCCTCCCAC AGCCGCCACC TTGCGAAGGA GACAATGATG AAGAAGAACC AGCCAAGTTA
    AAAGTGGAGC ACCATGATCT TTCAGTCGCT GGTCTGCAAA GTCCAGACAG AGTTTTGGGA
    TTAGAATCTC CCTTAATTTC GTCAAAGCCT CAGTCTCATT CACTGAATAC CCCTGGCAAA
    TCAGCGGCAG AACATCTCTT TGTGACCGCA ACACAGTTTG CAAAGGAACA GCATGCAAAT
    GGGGCACTCA AGGAAGGTGA TGGCGGGTAT CCAGTCACAA TCCCAGGTCC ACACTTGCCT
    GTGTCAGAGG AGCGGTGGGC ATTGGACGCA CTGAGAAATT TGGGCTTGTT GAAGCAGTTG
    CTGGTACAGC AGTTAGGTCT CACCGAGAAG AGTACTCAGG AAGACTGGCA GTCCTTCTCA
    CGATACGGGC CAGCTTCTGA AGAGGTACAG GCAGACAGTG GCATCCGGGA CTTGGAAAAT
    GTTAAGGCTT GTCATGCCAG GGAAGGACAG ATGTCTTTTA AAACTGGAAC TGGTGATATT
    GCAACTTGTG ACAGTCCACG GACTTCAACT GAGTCTTGTG CTGCACAGGA TTCAGTGATA
    CTGGCATCCC AAGACAGCCA GGCAAGTAAC GTTTTAGTAA TGGACCACAT GATCCTGACC
    CCAGAGATGC CTCCTGCAGA GCCAGAAGGG GGTCTGGATG AGAGTGGAGA GCACTTTTTT
    GATGCCCGTG AAGCACATAG TGATGATAAT CCATCAGAAG GTGATGGAGC AGTTAAAAAG
    GAAGAGAAGG ATGTTAATTT ACGCATCTCA GGAAACTGTT TGATCCTTGA TGGCTATGAT
    GCAGTTCAGG AGAGCTCCAC AGATGAGGAG GTTGCCTCCT CGTTCCCCCT GCAGCCTGTG
    ACGGGCATCC CCGCTGTGGA CTCCAGCCAC CAGCAGCAAC ATTCCCCTCA GAATGTGCAT
    CCCGAGGGGC CGGTTTCACC ATTCACCCCA GAATTCCTGG TCCAGCGGCA CTGGCGAGCT
    ATGGAGGATA CCTGTTTTGA GATCCAGAGT CCCTCATGTA CAGATTCACA AAGCCAAATC
    CTGGAGTACA TTCATAAAAT AGAGGCTGAC CTGGAACACT TAAAGAAGGT GGAGGAAAGT
    TACGCTCTTC TTTGCCAAAG GCTGGCTGGC TCAGCCCTCC CCGACAAGCT CTCAGATAAA
    AGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu90246
    Clone ID Related Accession (Same CDS sequence) XM_030244572.1 , XM_030244573.1
    Accession Version XM_030244572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT GAGCCGCAGT CGCTGGTCGG CAGTCCCTCC
    ACCCGGGGCG CCCCTCACAT CATTGGAGCA GAGGATGATG ATTTTGGTAC TGAGCATGAG
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TGCTGAAGTC TCGCCCTGCT
    CACTTGGCTG TTTTCTTACA CCATGTAGTC TCACAGTTTG ACCCTGCAAC ATTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAGCAGACA AACTCCAAGG AGACTCGGCG TGTCTTCCTG
    GAGTTTCATC AGTTCTTCTT GGACCGGTCT GCACACCTGA AGGTTCCAGT TCCTGAGGAA
    ATCTCCGTGG ATCTAGAAAA GAGAAGACCT GAGCTTATTC CTGAGGATCT GCATCGCCTC
    TACATTCAGA CAATGCAGGA GAGAGTCCAC CCTGAAGTTC AGAGGCACTT GGAGGATTTC
    CGACAGAAAC GCAGCATGGG GCTGACCTTG GCTGAGAGTG AGCTGACTAA ACTTGATGCA
    GAGAGAGACA AGGACCGGGG AACTCTGGAG AAGGAGCGAG CATGTGCGGA ACAGATCGTT
    ACCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAG GAGTTCCACA
    ATGCAGTATG TAATTCTCAT GTATATGAAG TATTTGGGAG TAAAAGTGAA AGAACCTCGA
    AACTTGGAGC ACAAGCGGGG TCGGATCGGC TTCCTTCCAA AGATCAAGCA AAGCATGAAG
    AAAGATAGAG AGGGCGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT TTTGGGACCC
    CCAAGAAGAC CAAGCCGCCA CGACAACAGT GCAATTGGCA GGGCCATGGA GATACAGAAG
    TCACGCCACC CGAAGCACTT ATCCACTCCC TCGTCTGTGA GTCCTGAGCC TCAGGACCCT
    GCCAAGTTGC GCCAGAGTGG AGTAGCCAAT GAAGGAACAG ACACTGGATA CCTGCCCGCC
    AGTTCCATGT CCTCTGCCAC CTCAGGGACT GCTCTTTCCC AAGAGGGAGG GAGAGAGAAT
    GATACAGGAA CAAAACAAGT GGGAGAAGCA TCGGCGCCTG GAGACTGCTT GGACAGCACA
    CCTCGAGTCC CCACTACTGT TTTCGACTTC CCTCCTCCTC TATTAGATCA AGTGCAGGAG
    GAGGAGTGTG AAGTAGAGAG GGTGGCAGAA CATGGGACCC CAAAACCCTT TCGGAAGTTT
    GACAGCATCG CCTTTGGAGA AAGTCAGAGT GAGGATGAGC AGTTTGAAAA TGACCTGGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGCAGAGAAG TGTTACTGGG ACTGAAACCT
    TCTGAGATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAGAG AGCTCATGTC
    CGGACACTGA AGGTTCTTGA CCAAGTATTC TATCAGCGAG TGTCCAGAGA AGGGATTCTA
    TCACCTTCAG AACTACGCAA GATTTTTTCA AACTTGGAAG ATATCCTTCA ACTTCATGTT
    GGGTTGAATG AGCAAATGAA AGCTGTCCGA AAAAGGAATG AAACTTCTGT TATTGATCAT
    ATTGGGGAAG ATCTGCTCAT CTGGTTTAGT GGACCAGGAG AGGAGAAGCT GAAACATGCT
    GCTGCCACAT TCTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGCCAGAAA
    AAGGATTCTC GATTCCATAC TTTTGTGCAA GACGCTGAGA GTAACCCCCT GTGCCGTCGT
    CTTCAGCTGA AGGACATCAT TCCCACCCAA ATGCAGAGAC TCACTAAGTA CCCGCTTCTT
    TTGGATAACA TTGCCAAGTA CACAGAATGG CCTCCAGAAA GGGAAAAGGT GAAGAAGGCT
    GCAGACCATT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAGGGA GGCAGAAAAC
    AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTTGACACCT CCAATCTCAA GTTGTCCGAG
    TACCCAAATG TTGACGAGCT CAGGAATTTG GATTTAACAA AAAGAAAGAT GATTCATGAA
    GGGCCGTTGG TCTGGAAGGT GAATAGAGAT AAAAGCATCG ATCTTTATAC ACTGCTGCTG
    GAAGACATCC TTGTGCTGCT ACAGAAGCAG GATGACAGGC TGGTGCTAAG GTGCCACAGC
    AAGATTCTGG CATCCACAGC CGACAGCAAA CACACCTTCA GCCCTGTCAT TAAGCTGAGC
    ACGGTGCTGG TTCGGCAGGT GGCGACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCA
    GATAATGGAG CCCAAATTTA TGAATTGGTA GCACAGACGG TTTCTGAAAA GACAGTCTGG
    CAAGACCTCA TCTGTCGAAT GGCTGCGTCA GTGAAGGAGC AGTCTACAAA GCCGATTCCC
    CTCCCACAGC CGCCACCTTG CGAAGGAGAC AATGATGAAG AAGAACCAGC CAAGTTAAAA
    GTGGAGCACC ATGATCTTTC AGTCGCTGGT CTGCAAAGTC CAGACAGAGT TTTGGGATTA
    GAATCTCCCT TAATTTCGTC AAAGCCTCAG TCTCATTCAC TGAATACCCC TGGCAAATCA
    GCGGCAGAAC ATCTCTTTGT GACCGCAACA CAGTTTGCAA AGGAACAGCA TGCAAATGGG
    GCACTCAAGG AAGGTGATGG CGGGTATCCA GTCACAATCC CAGGTCCACA CTTGCCTGTG
    TCAGAGGAGC GGTGGGCATT GGACGCACTG AGAAATTTGG GCTTGTTGAA GCAGTTGCTG
    GTACAGCAGT TAGGTCTCAC CGAGAAGAGT ACTCAGGAAG ACTGGCAGTC CTTCTCACGA
    TACGGGCCAG CTTCTGAAGA GGTACAGGCA GACAGTGGCA TCCGGGACTT GGAAAATGTT
    AAGGCTTGTC ATGCCAGGGA AGGACAGATG TCTTTTAAAA CTGGAACTGG TGATATTGCA
    ACTTGTGACA GTCCACGGAC TTCAACTGAG TCTTGTGCTG CACAGGATTC AGTGATACTG
    GCATCCCAAG ACAGCCAGGC AAGTAACGTT TTAGTAATGG ACCACATGAT CCTGACCCCA
    GAGATGCCTC CTGCAGAGCC AGAAGGGGGT CTGGATGAGA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTGTTTGA TCCTTGATGG CTATGATGCA
    GTTCAGGAGA GCTCCACAGA TGAGGAGGTT GCCTCCTCGT TCCCCCTGCA GCCTGTGACG
    GGCATCCCCG CTGTGGACTC CAGCCACCAG CAGCAACATT CCCCTCAGAA TGTGCATCCC
    GAGGGGCCGG TTTCACCATT CACCCCAGAA TTCCTGGTCC AGCGGCACTG GCGAGCTATG
    GAGGATACCT GTTTTGAGAT CCAGAGTCCC TCATGTACAG ATTCACAAAG CCAAATCCTG
    GAGTACATTC ATAAAATAGA GGCTGACCTG GAACACTTAA AGAAGGTGGA GGAAAGTTAC
    GCTCTTCTTT GCCAAAGGCT GGCTGGCTCA GCCCTCCCCG ACAAGCTCTC AGATAAAAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100432.1
    CDS1531..5853
    Translation

    Target ORF information:

    RefSeq Version XM_030244572.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030244572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT GAGCCGCAGT CGCTGGTCGG CAGTCCCTCC
    ACCCGGGGCG CCCCTCACAT CATTGGAGCA GAGGATGATG ATTTTGGTAC TGAGCATGAG
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TGCTGAAGTC TCGCCCTGCT
    CACTTGGCTG TTTTCTTACA CCATGTAGTC TCACAGTTTG ACCCTGCAAC ATTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAGCAGACA AACTCCAAGG AGACTCGGCG TGTCTTCCTG
    GAGTTTCATC AGTTCTTCTT GGACCGGTCT GCACACCTGA AGGTTCCAGT TCCTGAGGAA
    ATCTCCGTGG ATCTAGAAAA GAGAAGACCT GAGCTTATTC CTGAGGATCT GCATCGCCTC
    TACATTCAGA CAATGCAGGA GAGAGTCCAC CCTGAAGTTC AGAGGCACTT GGAGGATTTC
    CGACAGAAAC GCAGCATGGG GCTGACCTTG GCTGAGAGTG AGCTGACTAA ACTTGATGCA
    GAGAGAGACA AGGACCGGGG AACTCTGGAG AAGGAGCGAG CATGTGCGGA ACAGATCGTT
    ACCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAG GAGTTCCACA
    ATGCAGTATG TAATTCTCAT GTATATGAAG TATTTGGGAG TAAAAGTGAA AGAACCTCGA
    AACTTGGAGC ACAAGCGGGG TCGGATCGGC TTCCTTCCAA AGATCAAGCA AAGCATGAAG
    AAAGATAGAG AGGGCGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT TTTGGGACCC
    CCAAGAAGAC CAAGCCGCCA CGACAACAGT GCAATTGGCA GGGCCATGGA GATACAGAAG
    TCACGCCACC CGAAGCACTT ATCCACTCCC TCGTCTGTGA GTCCTGAGCC TCAGGACCCT
    GCCAAGTTGC GCCAGAGTGG AGTAGCCAAT GAAGGAACAG ACACTGGATA CCTGCCCGCC
    AGTTCCATGT CCTCTGCCAC CTCAGGGACT GCTCTTTCCC AAGAGGGAGG GAGAGAGAAT
    GATACAGGAA CAAAACAAGT GGGAGAAGCA TCGGCGCCTG GAGACTGCTT GGACAGCACA
    CCTCGAGTCC CCACTACTGT TTTCGACTTC CCTCCTCCTC TATTAGATCA AGTGCAGGAG
    GAGGAGTGTG AAGTAGAGAG GGTGGCAGAA CATGGGACCC CAAAACCCTT TCGGAAGTTT
    GACAGCATCG CCTTTGGAGA AAGTCAGAGT GAGGATGAGC AGTTTGAAAA TGACCTGGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGCAGAGAAG TGTTACTGGG ACTGAAACCT
    TCTGAGATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAGAG AGCTCATGTC
    CGGACACTGA AGGTTCTTGA CCAAGTATTC TATCAGCGAG TGTCCAGAGA AGGGATTCTA
    TCACCTTCAG AACTACGCAA GATTTTTTCA AACTTGGAAG ATATCCTTCA ACTTCATGTT
    GGGTTGAATG AGCAAATGAA AGCTGTCCGA AAAAGGAATG AAACTTCTGT TATTGATCAT
    ATTGGGGAAG ATCTGCTCAT CTGGTTTAGT GGACCAGGAG AGGAGAAGCT GAAACATGCT
    GCTGCCACAT TCTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGCCAGAAA
    AAGGATTCTC GATTCCATAC TTTTGTGCAA GACGCTGAGA GTAACCCCCT GTGCCGTCGT
    CTTCAGCTGA AGGACATCAT TCCCACCCAA ATGCAGAGAC TCACTAAGTA CCCGCTTCTT
    TTGGATAACA TTGCCAAGTA CACAGAATGG CCTCCAGAAA GGGAAAAGGT GAAGAAGGCT
    GCAGACCATT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAGGGA GGCAGAAAAC
    AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTTGACACCT CCAATCTCAA GTTGTCCGAG
    TACCCAAATG TTGACGAGCT CAGGAATTTG GATTTAACAA AAAGAAAGAT GATTCATGAA
    GGGCCGTTGG TCTGGAAGGT GAATAGAGAT AAAAGCATCG ATCTTTATAC ACTGCTGCTG
    GAAGACATCC TTGTGCTGCT ACAGAAGCAG GATGACAGGC TGGTGCTAAG GTGCCACAGC
    AAGATTCTGG CATCCACAGC CGACAGCAAA CACACCTTCA GCCCTGTCAT TAAGCTGAGC
    ACGGTGCTGG TTCGGCAGGT GGCGACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCA
    GATAATGGAG CCCAAATTTA TGAATTGGTA GCACAGACGG TTTCTGAAAA GACAGTCTGG
    CAAGACCTCA TCTGTCGAAT GGCTGCGTCA GTGAAGGAGC AGTCTACAAA GCCGATTCCC
    CTCCCACAGC CGCCACCTTG CGAAGGAGAC AATGATGAAG AAGAACCAGC CAAGTTAAAA
    GTGGAGCACC ATGATCTTTC AGTCGCTGGT CTGCAAAGTC CAGACAGAGT TTTGGGATTA
    GAATCTCCCT TAATTTCGTC AAAGCCTCAG TCTCATTCAC TGAATACCCC TGGCAAATCA
    GCGGCAGAAC ATCTCTTTGT GACCGCAACA CAGTTTGCAA AGGAACAGCA TGCAAATGGG
    GCACTCAAGG AAGGTGATGG CGGGTATCCA GTCACAATCC CAGGTCCACA CTTGCCTGTG
    TCAGAGGAGC GGTGGGCATT GGACGCACTG AGAAATTTGG GCTTGTTGAA GCAGTTGCTG
    GTACAGCAGT TAGGTCTCAC CGAGAAGAGT ACTCAGGAAG ACTGGCAGTC CTTCTCACGA
    TACGGGCCAG CTTCTGAAGA GGTACAGGCA GACAGTGGCA TCCGGGACTT GGAAAATGTT
    AAGGCTTGTC ATGCCAGGGA AGGACAGATG TCTTTTAAAA CTGGAACTGG TGATATTGCA
    ACTTGTGACA GTCCACGGAC TTCAACTGAG TCTTGTGCTG CACAGGATTC AGTGATACTG
    GCATCCCAAG ACAGCCAGGC AAGTAACGTT TTAGTAATGG ACCACATGAT CCTGACCCCA
    GAGATGCCTC CTGCAGAGCC AGAAGGGGGT CTGGATGAGA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTGTTTGA TCCTTGATGG CTATGATGCA
    GTTCAGGAGA GCTCCACAGA TGAGGAGGTT GCCTCCTCGT TCCCCCTGCA GCCTGTGACG
    GGCATCCCCG CTGTGGACTC CAGCCACCAG CAGCAACATT CCCCTCAGAA TGTGCATCCC
    GAGGGGCCGG TTTCACCATT CACCCCAGAA TTCCTGGTCC AGCGGCACTG GCGAGCTATG
    GAGGATACCT GTTTTGAGAT CCAGAGTCCC TCATGTACAG ATTCACAAAG CCAAATCCTG
    GAGTACATTC ATAAAATAGA GGCTGACCTG GAACACTTAA AGAAGGTGGA GGAAAGTTAC
    GCTCTTCTTT GCCAAAGGCT GGCTGGCTCA GCCCTCCCCG ACAAGCTCTC AGATAAAAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu90246
    Clone ID Related Accession (Same CDS sequence) XM_030244572.1 , XM_030244573.1
    Accession Version XM_030244573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT GAGCCGCAGT CGCTGGTCGG CAGTCCCTCC
    ACCCGGGGCG CCCCTCACAT CATTGGAGCA GAGGATGATG ATTTTGGTAC TGAGCATGAG
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TGCTGAAGTC TCGCCCTGCT
    CACTTGGCTG TTTTCTTACA CCATGTAGTC TCACAGTTTG ACCCTGCAAC ATTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAGCAGACA AACTCCAAGG AGACTCGGCG TGTCTTCCTG
    GAGTTTCATC AGTTCTTCTT GGACCGGTCT GCACACCTGA AGGTTCCAGT TCCTGAGGAA
    ATCTCCGTGG ATCTAGAAAA GAGAAGACCT GAGCTTATTC CTGAGGATCT GCATCGCCTC
    TACATTCAGA CAATGCAGGA GAGAGTCCAC CCTGAAGTTC AGAGGCACTT GGAGGATTTC
    CGACAGAAAC GCAGCATGGG GCTGACCTTG GCTGAGAGTG AGCTGACTAA ACTTGATGCA
    GAGAGAGACA AGGACCGGGG AACTCTGGAG AAGGAGCGAG CATGTGCGGA ACAGATCGTT
    ACCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAG GAGTTCCACA
    ATGCAGTATG TAATTCTCAT GTATATGAAG TATTTGGGAG TAAAAGTGAA AGAACCTCGA
    AACTTGGAGC ACAAGCGGGG TCGGATCGGC TTCCTTCCAA AGATCAAGCA AAGCATGAAG
    AAAGATAGAG AGGGCGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT TTTGGGACCC
    CCAAGAAGAC CAAGCCGCCA CGACAACAGT GCAATTGGCA GGGCCATGGA GATACAGAAG
    TCACGCCACC CGAAGCACTT ATCCACTCCC TCGTCTGTGA GTCCTGAGCC TCAGGACCCT
    GCCAAGTTGC GCCAGAGTGG AGTAGCCAAT GAAGGAACAG ACACTGGATA CCTGCCCGCC
    AGTTCCATGT CCTCTGCCAC CTCAGGGACT GCTCTTTCCC AAGAGGGAGG GAGAGAGAAT
    GATACAGGAA CAAAACAAGT GGGAGAAGCA TCGGCGCCTG GAGACTGCTT GGACAGCACA
    CCTCGAGTCC CCACTACTGT TTTCGACTTC CCTCCTCCTC TATTAGATCA AGTGCAGGAG
    GAGGAGTGTG AAGTAGAGAG GGTGGCAGAA CATGGGACCC CAAAACCCTT TCGGAAGTTT
    GACAGCATCG CCTTTGGAGA AAGTCAGAGT GAGGATGAGC AGTTTGAAAA TGACCTGGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGCAGAGAAG TGTTACTGGG ACTGAAACCT
    TCTGAGATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAGAG AGCTCATGTC
    CGGACACTGA AGGTTCTTGA CCAAGTATTC TATCAGCGAG TGTCCAGAGA AGGGATTCTA
    TCACCTTCAG AACTACGCAA GATTTTTTCA AACTTGGAAG ATATCCTTCA ACTTCATGTT
    GGGTTGAATG AGCAAATGAA AGCTGTCCGA AAAAGGAATG AAACTTCTGT TATTGATCAT
    ATTGGGGAAG ATCTGCTCAT CTGGTTTAGT GGACCAGGAG AGGAGAAGCT GAAACATGCT
    GCTGCCACAT TCTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGCCAGAAA
    AAGGATTCTC GATTCCATAC TTTTGTGCAA GACGCTGAGA GTAACCCCCT GTGCCGTCGT
    CTTCAGCTGA AGGACATCAT TCCCACCCAA ATGCAGAGAC TCACTAAGTA CCCGCTTCTT
    TTGGATAACA TTGCCAAGTA CACAGAATGG CCTCCAGAAA GGGAAAAGGT GAAGAAGGCT
    GCAGACCATT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAGGGA GGCAGAAAAC
    AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTTGACACCT CCAATCTCAA GTTGTCCGAG
    TACCCAAATG TTGACGAGCT CAGGAATTTG GATTTAACAA AAAGAAAGAT GATTCATGAA
    GGGCCGTTGG TCTGGAAGGT GAATAGAGAT AAAAGCATCG ATCTTTATAC ACTGCTGCTG
    GAAGACATCC TTGTGCTGCT ACAGAAGCAG GATGACAGGC TGGTGCTAAG GTGCCACAGC
    AAGATTCTGG CATCCACAGC CGACAGCAAA CACACCTTCA GCCCTGTCAT TAAGCTGAGC
    ACGGTGCTGG TTCGGCAGGT GGCGACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCA
    GATAATGGAG CCCAAATTTA TGAATTGGTA GCACAGACGG TTTCTGAAAA GACAGTCTGG
    CAAGACCTCA TCTGTCGAAT GGCTGCGTCA GTGAAGGAGC AGTCTACAAA GCCGATTCCC
    CTCCCACAGC CGCCACCTTG CGAAGGAGAC AATGATGAAG AAGAACCAGC CAAGTTAAAA
    GTGGAGCACC ATGATCTTTC AGTCGCTGGT CTGCAAAGTC CAGACAGAGT TTTGGGATTA
    GAATCTCCCT TAATTTCGTC AAAGCCTCAG TCTCATTCAC TGAATACCCC TGGCAAATCA
    GCGGCAGAAC ATCTCTTTGT GACCGCAACA CAGTTTGCAA AGGAACAGCA TGCAAATGGG
    GCACTCAAGG AAGGTGATGG CGGGTATCCA GTCACAATCC CAGGTCCACA CTTGCCTGTG
    TCAGAGGAGC GGTGGGCATT GGACGCACTG AGAAATTTGG GCTTGTTGAA GCAGTTGCTG
    GTACAGCAGT TAGGTCTCAC CGAGAAGAGT ACTCAGGAAG ACTGGCAGTC CTTCTCACGA
    TACGGGCCAG CTTCTGAAGA GGTACAGGCA GACAGTGGCA TCCGGGACTT GGAAAATGTT
    AAGGCTTGTC ATGCCAGGGA AGGACAGATG TCTTTTAAAA CTGGAACTGG TGATATTGCA
    ACTTGTGACA GTCCACGGAC TTCAACTGAG TCTTGTGCTG CACAGGATTC AGTGATACTG
    GCATCCCAAG ACAGCCAGGC AAGTAACGTT TTAGTAATGG ACCACATGAT CCTGACCCCA
    GAGATGCCTC CTGCAGAGCC AGAAGGGGGT CTGGATGAGA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTGTTTGA TCCTTGATGG CTATGATGCA
    GTTCAGGAGA GCTCCACAGA TGAGGAGGTT GCCTCCTCGT TCCCCCTGCA GCCTGTGACG
    GGCATCCCCG CTGTGGACTC CAGCCACCAG CAGCAACATT CCCCTCAGAA TGTGCATCCC
    GAGGGGCCGG TTTCACCATT CACCCCAGAA TTCCTGGTCC AGCGGCACTG GCGAGCTATG
    GAGGATACCT GTTTTGAGAT CCAGAGTCCC TCATGTACAG ATTCACAAAG CCAAATCCTG
    GAGTACATTC ATAAAATAGA GGCTGACCTG GAACACTTAA AGAAGGTGGA GGAAAGTTAC
    GCTCTTCTTT GCCAAAGGCT GGCTGGCTCA GCCCTCCCCG ACAAGCTCTC AGATAAAAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100433.1
    CDS1474..5796
    Translation

    Target ORF information:

    RefSeq Version XM_030244573.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030244573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGCGTGCTG GAGTACAAAC AGGTGATCGA ATCATCAAGG TGAATGGTAC TCTGGTGACT 
    CATTCAAATC ATCTGGAGGT GGTGAAACTA ATCAGATCTG GTTCCTATGT AGCTCTCACT
    GTCCAGGGAC GCCCACCTGG GTCGCCCCAG ATCCCACTTG CTGATTCTGA AGTAGAGCCA
    TCAGTCACTG GACATATGTC TCCCATTATG ACATCCCCTC ATTCCCCTGG AGCAGCCGGG
    AATATGGAGA GAATCACCAG TCCTGTGCTC GTGGGGGAGG AAAACAATGT GGTCCATAAC
    CAGAAAGTAG AGATTCTGAG AAAGATGTTA CAGAAGGAAC AGGAACGGCT GCAGTTGCTG
    CAGGAAGATT ACAACCGAAC GGCTACCCAA AGATTGCTCA AGGAGATCCA GGAGGCCAAG
    AAGCACATCC CGCAGCTGCA GGAGCAGCTG TCCAAAGCCA CGGGCTCTGC TCAGGACGGA
    GCTGTAATTG CACCCTCCAG GCCATTAGGT GATGCCCTAA CACTCAGTGA GGCAGAAGCA
    GATCCTGGAG ATGGCCTGTG CAGGACTGAC TGGAGCAGTG GAGATGCTTC TCGGCCCAGT
    AGTGACAGTG CTGATAGTCC CAAGAGTAGC CTGAGAGAAA GGAGTTACCT GGAGGAAGCC
    CCAGAGAGAA GTGAAGGAGT TCAGGACGCT GAGCCGCAGT CGCTGGTCGG CAGTCCCTCC
    ACCCGGGGCG CCCCTCACAT CATTGGAGCA GAGGATGATG ATTTTGGTAC TGAGCATGAG
    CAGATCAATG GACAGTGCAG CTGTTTCCAG AGCATTGAAT TGCTGAAGTC TCGCCCTGCT
    CACTTGGCTG TTTTCTTACA CCATGTAGTC TCACAGTTTG ACCCTGCAAC ATTGCTCTGT
    TATCTCTATT CAGACCTGTA TAAGCAGACA AACTCCAAGG AGACTCGGCG TGTCTTCCTG
    GAGTTTCATC AGTTCTTCTT GGACCGGTCT GCACACCTGA AGGTTCCAGT TCCTGAGGAA
    ATCTCCGTGG ATCTAGAAAA GAGAAGACCT GAGCTTATTC CTGAGGATCT GCATCGCCTC
    TACATTCAGA CAATGCAGGA GAGAGTCCAC CCTGAAGTTC AGAGGCACTT GGAGGATTTC
    CGACAGAAAC GCAGCATGGG GCTGACCTTG GCTGAGAGTG AGCTGACTAA ACTTGATGCA
    GAGAGAGACA AGGACCGGGG AACTCTGGAG AAGGAGCGAG CATGTGCGGA ACAGATCGTT
    ACCAAAATTG AAGAAGTGTT GATGACTGCT CAGGCTGTAG AAGAAGAAAG GAGTTCCACA
    ATGCAGTATG TAATTCTCAT GTATATGAAG TATTTGGGAG TAAAAGTGAA AGAACCTCGA
    AACTTGGAGC ACAAGCGGGG TCGGATCGGC TTCCTTCCAA AGATCAAGCA AAGCATGAAG
    AAAGATAGAG AGGGCGAAGA AAAAGGGAAG CGAAGAGGAT TCCCCAGCAT TTTGGGACCC
    CCAAGAAGAC CAAGCCGCCA CGACAACAGT GCAATTGGCA GGGCCATGGA GATACAGAAG
    TCACGCCACC CGAAGCACTT ATCCACTCCC TCGTCTGTGA GTCCTGAGCC TCAGGACCCT
    GCCAAGTTGC GCCAGAGTGG AGTAGCCAAT GAAGGAACAG ACACTGGATA CCTGCCCGCC
    AGTTCCATGT CCTCTGCCAC CTCAGGGACT GCTCTTTCCC AAGAGGGAGG GAGAGAGAAT
    GATACAGGAA CAAAACAAGT GGGAGAAGCA TCGGCGCCTG GAGACTGCTT GGACAGCACA
    CCTCGAGTCC CCACTACTGT TTTCGACTTC CCTCCTCCTC TATTAGATCA AGTGCAGGAG
    GAGGAGTGTG AAGTAGAGAG GGTGGCAGAA CATGGGACCC CAAAACCCTT TCGGAAGTTT
    GACAGCATCG CCTTTGGAGA AAGTCAGAGT GAGGATGAGC AGTTTGAAAA TGACCTGGAG
    ACAGATCCAC CCAACTGGCA GCAGCTTGTT AGCAGAGAAG TGTTACTGGG ACTGAAACCT
    TCTGAGATCA AAAGACAGGA AGTGATTAAT GAATTGTTCT ACACTGAGAG AGCTCATGTC
    CGGACACTGA AGGTTCTTGA CCAAGTATTC TATCAGCGAG TGTCCAGAGA AGGGATTCTA
    TCACCTTCAG AACTACGCAA GATTTTTTCA AACTTGGAAG ATATCCTTCA ACTTCATGTT
    GGGTTGAATG AGCAAATGAA AGCTGTCCGA AAAAGGAATG AAACTTCTGT TATTGATCAT
    ATTGGGGAAG ATCTGCTCAT CTGGTTTAGT GGACCAGGAG AGGAGAAGCT GAAACATGCT
    GCTGCCACAT TCTGCAGTAA CCAACCTTTC GCCCTGGAAA TGATCAAGTC TCGCCAGAAA
    AAGGATTCTC GATTCCATAC TTTTGTGCAA GACGCTGAGA GTAACCCCCT GTGCCGTCGT
    CTTCAGCTGA AGGACATCAT TCCCACCCAA ATGCAGAGAC TCACTAAGTA CCCGCTTCTT
    TTGGATAACA TTGCCAAGTA CACAGAATGG CCTCCAGAAA GGGAAAAGGT GAAGAAGGCT
    GCAGACCATT GTCGTCAGAT CTTAAATTAT GTAAATCAGG CTGTCAGGGA GGCAGAAAAC
    AAGCAGCGCT TAGAAGACTA TCAGCGTCGC CTTGACACCT CCAATCTCAA GTTGTCCGAG
    TACCCAAATG TTGACGAGCT CAGGAATTTG GATTTAACAA AAAGAAAGAT GATTCATGAA
    GGGCCGTTGG TCTGGAAGGT GAATAGAGAT AAAAGCATCG ATCTTTATAC ACTGCTGCTG
    GAAGACATCC TTGTGCTGCT ACAGAAGCAG GATGACAGGC TGGTGCTAAG GTGCCACAGC
    AAGATTCTGG CATCCACAGC CGACAGCAAA CACACCTTCA GCCCTGTCAT TAAGCTGAGC
    ACGGTGCTGG TTCGGCAGGT GGCGACAGAT AACAAAGCTT TATTCGTCAT CTCCATGTCA
    GATAATGGAG CCCAAATTTA TGAATTGGTA GCACAGACGG TTTCTGAAAA GACAGTCTGG
    CAAGACCTCA TCTGTCGAAT GGCTGCGTCA GTGAAGGAGC AGTCTACAAA GCCGATTCCC
    CTCCCACAGC CGCCACCTTG CGAAGGAGAC AATGATGAAG AAGAACCAGC CAAGTTAAAA
    GTGGAGCACC ATGATCTTTC AGTCGCTGGT CTGCAAAGTC CAGACAGAGT TTTGGGATTA
    GAATCTCCCT TAATTTCGTC AAAGCCTCAG TCTCATTCAC TGAATACCCC TGGCAAATCA
    GCGGCAGAAC ATCTCTTTGT GACCGCAACA CAGTTTGCAA AGGAACAGCA TGCAAATGGG
    GCACTCAAGG AAGGTGATGG CGGGTATCCA GTCACAATCC CAGGTCCACA CTTGCCTGTG
    TCAGAGGAGC GGTGGGCATT GGACGCACTG AGAAATTTGG GCTTGTTGAA GCAGTTGCTG
    GTACAGCAGT TAGGTCTCAC CGAGAAGAGT ACTCAGGAAG ACTGGCAGTC CTTCTCACGA
    TACGGGCCAG CTTCTGAAGA GGTACAGGCA GACAGTGGCA TCCGGGACTT GGAAAATGTT
    AAGGCTTGTC ATGCCAGGGA AGGACAGATG TCTTTTAAAA CTGGAACTGG TGATATTGCA
    ACTTGTGACA GTCCACGGAC TTCAACTGAG TCTTGTGCTG CACAGGATTC AGTGATACTG
    GCATCCCAAG ACAGCCAGGC AAGTAACGTT TTAGTAATGG ACCACATGAT CCTGACCCCA
    GAGATGCCTC CTGCAGAGCC AGAAGGGGGT CTGGATGAGA GTGGAGAGCA CTTTTTTGAT
    GCCCGTGAAG CACATAGTGA TGATAATCCA TCAGAAGGTG ATGGAGCAGT TAAAAAGGAA
    GAGAAGGATG TTAATTTACG CATCTCAGGA AACTGTTTGA TCCTTGATGG CTATGATGCA
    GTTCAGGAGA GCTCCACAGA TGAGGAGGTT GCCTCCTCGT TCCCCCTGCA GCCTGTGACG
    GGCATCCCCG CTGTGGACTC CAGCCACCAG CAGCAACATT CCCCTCAGAA TGTGCATCCC
    GAGGGGCCGG TTTCACCATT CACCCCAGAA TTCCTGGTCC AGCGGCACTG GCGAGCTATG
    GAGGATACCT GTTTTGAGAT CCAGAGTCCC TCATGTACAG ATTCACAAAG CCAAATCCTG
    GAGTACATTC ATAAAATAGA GGCTGACCTG GAACACTTAA AGAAGGTGGA GGAAAGTTAC
    GCTCTTCTTT GCCAAAGGCT GGCTGGCTCA GCCCTCCCCG ACAAGCTCTC AGATAAAAGT
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu70443
    Clone ID Related Accession (Same CDS sequence) XM_011242597.1 , XM_011242597.2
    Accession Version XM_011242597.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011242597.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTGAGCCGCA GTCGCTGGTC
    GGCAGTCCCT CCACCCGGGG CGCCCCTCAC ATCATTGGAG CAGAGGATGA TGATTTTGGT
    ACTGAGCATG AGCAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTGCTGAAG
    TCTCGCCCTG CTCACTTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCTGCA
    ACATTGCTCT GTTATCTCTA TTCAGACCTG TATAAGCAGA CAAACTCCAA GGAGACTCGG
    CGTGTCTTCC TGGAGTTTCA TCAGTTCTTC TTGGACCGGT CTGCACACCT GAAGGTTCCA
    GTTCCTGAGG AAATCTCCGT GGATCTAGAA AAGAGAAGAC CTGAGCTTAT TCCTGAGGAT
    CTGCATCGCC TCTACATTCA GACAATGCAG GAGAGAGTCC ACCCTGAAGT TCAGAGGCAC
    TTGGAGGATT TCCGACAGAA ACGCAGCATG GGGCTGACCT TGGCTGAGAG TGAGCTGACT
    AAACTTGATG CAGAGAGAGA CAAGGACCGG GGAACTCTGG AGAAGGAGCG AGCATGTGCG
    GAACAGATCG TTACCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA
    AGGAGTTCCA CAATGCAGTA TGTAATTCTC ATGTATATGA AGTATTTGGG AGTAAAAGTG
    AAAGAACCTC GAAACTTGGA GCACAAGCGG GGTCGGATCG GCTTCCTTCC AAAGATCAAG
    CAAAGCATGA AGAAAGATAG AGAGGGCGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATTTTGGGAC CCCCAAGAAG ACCAAGCCGC CACGACAACA GTGCAATTGG CAGGGCCATG
    GAGATACAGA AGTCACGCCA CCCGAAGCAC TTATCCACTC CCTCGTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GGAGTAGCCA ATGAAGGAAC AGACACTGGA
    TACCTGCCCG CCAGTTCCAT GTCCTCTGCC ACCTCAGGGA CTGCTCTTTC CCAAGAGGGA
    GGGAGAGAGA ATGATACAGG AACAAAACAA GTGGGAGAAG CATCGGCGCC TGGAGACTGC
    TTGGACAGCA CACCTCGAGT CCCCACTACT GTTTTCGACT TCCCTCCTCC TCTATTAGAT
    CAAGTGCAGG AGGAGGAGTG TGAAGTAGAG AGGGTGGCAG AACATGGGAC CCCAAAACCC
    TTTCGGAAGT TTGACAGCAT CGCCTTTGGA GAAAGTCAGA GTGAGGATGA GCAGTTTGAA
    AATGACCTGG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGCAGAGA AGTGTTACTG
    GGACTGAAAC CTTCTGAGAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAG
    AGAGCTCATG TCCGGACACT GAAGGTTCTT GACCAAGTAT TCTATCAGCG AGTGTCCAGA
    GAAGGGATTC TATCACCTTC AGAACTACGC AAGATTTTTT CAAACTTGGA AGATATCCTT
    CAACTTCATG TTGGGTTGAA TGAGCAAATG AAAGCTGTCC GAAAAAGGAA TGAAACTTCT
    GTTATTGATC ATATTGGGGA AGATCTGCTC ATCTGGTTTA GTGGACCAGG AGAGGAGAAG
    CTGAAACATG CTGCTGCCAC ATTCTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG
    TCTCGCCAGA AAAAGGATTC TCGATTCCAT ACTTTTGTGC AAGACGCTGA GAGTAACCCC
    CTGTGCCGTC GTCTTCAGCT GAAGGACATC ATTCCCACCC AAATGCAGAG ACTCACTAAG
    TACCCGCTTC TTTTGGATAA CATTGCCAAG TACACAGAAT GGCCTCCAGA AAGGGAAAAG
    GTGAAGAAGG CTGCAGACCA TTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAGG
    GAGGCAGAAA ACAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTTGACAC CTCCAATCTC
    AAGTTGTCCG AGTACCCAAA TGTTGACGAG CTCAGGAATT TGGATTTAAC AAAAAGAAAG
    ATGATTCATG AAGGGCCGTT GGTCTGGAAG GTGAATAGAG ATAAAAGCAT CGATCTTTAT
    ACACTGCTGC TGGAAGACAT CCTTGTGCTG CTACAGAAGC AGGATGACAG GCTGGTGCTA
    AGGTGCCACA GCAAGATTCT GGCATCCACA GCCGACAGCA AACACACCTT CAGCCCTGTC
    ATTAAGCTGA GCACGGTGCT GGTTCGGCAG GTGGCGACAG ATAACAAAGC TTTATTCGTC
    ATCTCCATGT CAGATAATGG AGCCCAAATT TATGAATTGG TAGCACAGAC GGTTTCTGAA
    AAGACAGTCT GGCAAGACCT CATCTGTCGA ATGGCTGCGT CAGTGAAGGA GCAGTCTACA
    AAGCCGATTC CCCTCCCACA GCCGCCACCT TGCGAAGGAG ACAATGATGA AGAAGAACCA
    GCCAAGTTAA AAGTGGAGCA CCATGATCTT TCAGTCGCTG GTCTGCAAAG TCCAGACAGA
    GTTTTGGGAT TAGAATCTCC CTTAATTTCG TCAAAGCCTC AGTCTCATTC ACTGAATACC
    CCTGGCAAAT CAGCGGCAGA ACATCTCTTT GTGACCGCAA CACAGTTTGC AAAGGAACAG
    CATGCAAATG GGGCACTCAA GGAAGGTGAT GGCGGGTATC CAGTCACAAT CCCAGGTCCA
    CACTTGCCTG TGTCAGAGGA GCGGTGGGCA TTGGACGCAC TGAGAAATTT GGGCTTGTTG
    AAGCAGTTGC TGGTACAGCA GTTAGGTCTC ACCGAGAAGA GTACTCAGGA AGACTGGCAG
    TCCTTCTCAC GATACGGGCC AGCTTCTGAA GAGGTACAGG CAGACAGTGG CATCCGGGAC
    TTGGAAAATG TTAAGGCTTG TCATGCCAGG GAAGGACAGA TGTCTTTTAA AACTGGAACT
    GGTGATATTG CAACTTGTGA CAGTCCACGG ACTTCAACTG AGTCTTGTGC TGCACAGGAT
    TCAGTGATAC TGGCATCCCA AGACAGCCAG GCAAGTAACG TTTTAGTAAT GGACCACATG
    ATCCTGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGG GTCTGGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTGTTT GATCCTTGAT
    GGCTATGATG CAGTTCAGGA GAGCTCCACA GATGAGGAGG TTGCCTCCTC GTTCCCCCTG
    CAGCCTGTGA CGGGCATCCC CGCTGTGGAC TCCAGCCACC AGCAGCAACA TTCCCCTCAG
    AATGTGCATC CCGAGGGGCC GGTTTCACCA TTCACCCCAG AATTCCTGGT CCAGCGGCAC
    TGGCGAGCTA TGGAGGATAC CTGTTTTGAG ATCCAGAGTC CCTCATGTAC AGATTCACAA
    AGCCAAATCC TGGAGTACAT TCATAAAATA GAGGCTGACC TGGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACGCTCTTCT TTGCCAAAGG CTGGCTGGCT CAGCCCTCCC CGACAAGCTC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011240899.1
    CDS45..4619
    Translation

    Target ORF information:

    RefSeq Version XM_011242597.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011242597.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTGAGCCGCA GTCGCTGGTC
    GGCAGTCCCT CCACCCGGGG CGCCCCTCAC ATCATTGGAG CAGAGGATGA TGATTTTGGT
    ACTGAGCATG AGCAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTGCTGAAG
    TCTCGCCCTG CTCACTTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCTGCA
    ACATTGCTCT GTTATCTCTA TTCAGACCTG TATAAGCAGA CAAACTCCAA GGAGACTCGG
    CGTGTCTTCC TGGAGTTTCA TCAGTTCTTC TTGGACCGGT CTGCACACCT GAAGGTTCCA
    GTTCCTGAGG AAATCTCCGT GGATCTAGAA AAGAGAAGAC CTGAGCTTAT TCCTGAGGAT
    CTGCATCGCC TCTACATTCA GACAATGCAG GAGAGAGTCC ACCCTGAAGT TCAGAGGCAC
    TTGGAGGATT TCCGACAGAA ACGCAGCATG GGGCTGACCT TGGCTGAGAG TGAGCTGACT
    AAACTTGATG CAGAGAGAGA CAAGGACCGG GGAACTCTGG AGAAGGAGCG AGCATGTGCG
    GAACAGATCG TTACCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA
    AGGAGTTCCA CAATGCAGTA TGTAATTCTC ATGTATATGA AGTATTTGGG AGTAAAAGTG
    AAAGAACCTC GAAACTTGGA GCACAAGCGG GGTCGGATCG GCTTCCTTCC AAAGATCAAG
    CAAAGCATGA AGAAAGATAG AGAGGGCGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATTTTGGGAC CCCCAAGAAG ACCAAGCCGC CACGACAACA GTGCAATTGG CAGGGCCATG
    GAGATACAGA AGTCACGCCA CCCGAAGCAC TTATCCACTC CCTCGTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GGAGTAGCCA ATGAAGGAAC AGACACTGGA
    TACCTGCCCG CCAGTTCCAT GTCCTCTGCC ACCTCAGGGA CTGCTCTTTC CCAAGAGGGA
    GGGAGAGAGA ATGATACAGG AACAAAACAA GTGGGAGAAG CATCGGCGCC TGGAGACTGC
    TTGGACAGCA CACCTCGAGT CCCCACTACT GTTTTCGACT TCCCTCCTCC TCTATTAGAT
    CAAGTGCAGG AGGAGGAGTG TGAAGTAGAG AGGGTGGCAG AACATGGGAC CCCAAAACCC
    TTTCGGAAGT TTGACAGCAT CGCCTTTGGA GAAAGTCAGA GTGAGGATGA GCAGTTTGAA
    AATGACCTGG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGCAGAGA AGTGTTACTG
    GGACTGAAAC CTTCTGAGAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAG
    AGAGCTCATG TCCGGACACT GAAGGTTCTT GACCAAGTAT TCTATCAGCG AGTGTCCAGA
    GAAGGGATTC TATCACCTTC AGAACTACGC AAGATTTTTT CAAACTTGGA AGATATCCTT
    CAACTTCATG TTGGGTTGAA TGAGCAAATG AAAGCTGTCC GAAAAAGGAA TGAAACTTCT
    GTTATTGATC ATATTGGGGA AGATCTGCTC ATCTGGTTTA GTGGACCAGG AGAGGAGAAG
    CTGAAACATG CTGCTGCCAC ATTCTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG
    TCTCGCCAGA AAAAGGATTC TCGATTCCAT ACTTTTGTGC AAGACGCTGA GAGTAACCCC
    CTGTGCCGTC GTCTTCAGCT GAAGGACATC ATTCCCACCC AAATGCAGAG ACTCACTAAG
    TACCCGCTTC TTTTGGATAA CATTGCCAAG TACACAGAAT GGCCTCCAGA AAGGGAAAAG
    GTGAAGAAGG CTGCAGACCA TTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAGG
    GAGGCAGAAA ACAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTTGACAC CTCCAATCTC
    AAGTTGTCCG AGTACCCAAA TGTTGACGAG CTCAGGAATT TGGATTTAAC AAAAAGAAAG
    ATGATTCATG AAGGGCCGTT GGTCTGGAAG GTGAATAGAG ATAAAAGCAT CGATCTTTAT
    ACACTGCTGC TGGAAGACAT CCTTGTGCTG CTACAGAAGC AGGATGACAG GCTGGTGCTA
    AGGTGCCACA GCAAGATTCT GGCATCCACA GCCGACAGCA AACACACCTT CAGCCCTGTC
    ATTAAGCTGA GCACGGTGCT GGTTCGGCAG GTGGCGACAG ATAACAAAGC TTTATTCGTC
    ATCTCCATGT CAGATAATGG AGCCCAAATT TATGAATTGG TAGCACAGAC GGTTTCTGAA
    AAGACAGTCT GGCAAGACCT CATCTGTCGA ATGGCTGCGT CAGTGAAGGA GCAGTCTACA
    AAGCCGATTC CCCTCCCACA GCCGCCACCT TGCGAAGGAG ACAATGATGA AGAAGAACCA
    GCCAAGTTAA AAGTGGAGCA CCATGATCTT TCAGTCGCTG GTCTGCAAAG TCCAGACAGA
    GTTTTGGGAT TAGAATCTCC CTTAATTTCG TCAAAGCCTC AGTCTCATTC ACTGAATACC
    CCTGGCAAAT CAGCGGCAGA ACATCTCTTT GTGACCGCAA CACAGTTTGC AAAGGAACAG
    CATGCAAATG GGGCACTCAA GGAAGGTGAT GGCGGGTATC CAGTCACAAT CCCAGGTCCA
    CACTTGCCTG TGTCAGAGGA GCGGTGGGCA TTGGACGCAC TGAGAAATTT GGGCTTGTTG
    AAGCAGTTGC TGGTACAGCA GTTAGGTCTC ACCGAGAAGA GTACTCAGGA AGACTGGCAG
    TCCTTCTCAC GATACGGGCC AGCTTCTGAA GAGGTACAGG CAGACAGTGG CATCCGGGAC
    TTGGAAAATG TTAAGGCTTG TCATGCCAGG GAAGGACAGA TGTCTTTTAA AACTGGAACT
    GGTGATATTG CAACTTGTGA CAGTCCACGG ACTTCAACTG AGTCTTGTGC TGCACAGGAT
    TCAGTGATAC TGGCATCCCA AGACAGCCAG GCAAGTAACG TTTTAGTAAT GGACCACATG
    ATCCTGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGG GTCTGGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTGTTT GATCCTTGAT
    GGCTATGATG CAGTTCAGGA GAGCTCCACA GATGAGGAGG TTGCCTCCTC GTTCCCCCTG
    CAGCCTGTGA CGGGCATCCC CGCTGTGGAC TCCAGCCACC AGCAGCAACA TTCCCCTCAG
    AATGTGCATC CCGAGGGGCC GGTTTCACCA TTCACCCCAG AATTCCTGGT CCAGCGGCAC
    TGGCGAGCTA TGGAGGATAC CTGTTTTGAG ATCCAGAGTC CCTCATGTAC AGATTCACAA
    AGCCAAATCC TGGAGTACAT TCATAAAATA GAGGCTGACC TGGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACGCTCTTCT TTGCCAAAGG CTGGCTGGCT CAGCCCTCCC CGACAAGCTC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82920
    Clone ID Related Accession (Same CDS sequence) XM_017313565.1 , XM_017313565.2
    Accession Version XM_017313565.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGCATGGAA GTATTTTGAA CCGAGAGTCA 
    CCAACAGATA AGAAACAGAA AGTTGAACGC AGTTCATCGC ATGATTTTGA CCCCACAGAT
    AGCTCCTCCA AGAAGACAAA GTCTAGTTCA GAGGAGAGTA GATCCGAGAT CTATGGTCTT
    GTTCAGCGCT GTGTGATCAT CCAGAAAGAC GACAATGGCT TCGGGCTGAC AGTCAGTGGA
    GACAATCCGG TCTTCGTACA ATCTGTCAAA GAAGATGGAG CAGCCATGCG TGCTGGAGTA
    CAAACAGGTG ATCGAATCAT CAAGGTGAAT GGTACTCTGG TGACTCATTC AAATCATCTG
    GAGGTGGTGA AACTAATCAG ATCTGGTTCC TATGTAGCTC TCACTGTCCA GGGACGCCCA
    CCTGGGTCGC CCCAGATCCC ACTTGCTGAT TCTGAAGTAG AGCCATCAGT CACTGGACAT
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169054.1
    CDS54..4664
    Translation

    Target ORF information:

    RefSeq Version XM_017313565.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGCATGGAA GTATTTTGAA CCGAGAGTCA 
    CCAACAGATA AGAAACAGAA AGTTGAACGC AGTTCATCGC ATGATTTTGA CCCCACAGAT
    AGCTCCTCCA AGAAGACAAA GTCTAGTTCA GAGGAGAGTA GATCCGAGAT CTATGGTCTT
    GTTCAGCGCT GTGTGATCAT CCAGAAAGAC GACAATGGCT TCGGGCTGAC AGTCAGTGGA
    GACAATCCGG TCTTCGTACA ATCTGTCAAA GAAGATGGAG CAGCCATGCG TGCTGGAGTA
    CAAACAGGTG ATCGAATCAT CAAGGTGAAT GGTACTCTGG TGACTCATTC AAATCATCTG
    GAGGTGGTGA AACTAATCAG ATCTGGTTCC TATGTAGCTC TCACTGTCCA GGGACGCCCA
    CCTGGGTCGC CCCAGATCCC ACTTGCTGAT TCTGAAGTAG AGCCATCAGT CACTGGACAT
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82920
    Clone ID Related Accession (Same CDS sequence) XM_017313565.1 , XM_017313565.2
    Accession Version XM_017313565.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313565.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGCATGGAA GTATTTTGAA CCGAGAGTCA 
    CCAACAGATA AGAAACAGAA AGTTGAACGC AGTTCATCGC ATGATTTTGA CCCCACAGAT
    AGCTCCTCCA AGAAGACAAA GTCTAGTTCA GAGGAGAGTA GATCCGAGAT CTATGGTCTT
    GTTCAGCGCT GTGTGATCAT CCAGAAAGAC GACAATGGCT TCGGGCTGAC AGTCAGTGGA
    GACAATCCGG TCTTCGTACA ATCTGTCAAA GAAGATGGAG CAGCCATGCG TGCTGGAGTA
    CAAACAGGTG ATCGAATCAT CAAGGTGAAT GGTACTCTGG TGACTCATTC AAATCATCTG
    GAGGTGGTGA AACTAATCAG ATCTGGTTCC TATGTAGCTC TCACTGTCCA GGGACGCCCA
    CCTGGGTCGC CCCAGATCCC ACTTGCTGAT TCTGAAGTAG AGCCATCAGT CACTGGACAT
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169054.1
    CDS54..4664
    Translation

    Target ORF information:

    RefSeq Version XM_017313565.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313565.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGCATGGAA GTATTTTGAA CCGAGAGTCA 
    CCAACAGATA AGAAACAGAA AGTTGAACGC AGTTCATCGC ATGATTTTGA CCCCACAGAT
    AGCTCCTCCA AGAAGACAAA GTCTAGTTCA GAGGAGAGTA GATCCGAGAT CTATGGTCTT
    GTTCAGCGCT GTGTGATCAT CCAGAAAGAC GACAATGGCT TCGGGCTGAC AGTCAGTGGA
    GACAATCCGG TCTTCGTACA ATCTGTCAAA GAAGATGGAG CAGCCATGCG TGCTGGAGTA
    CAAACAGGTG ATCGAATCAT CAAGGTGAAT GGTACTCTGG TGACTCATTC AAATCATCTG
    GAGGTGGTGA AACTAATCAG ATCTGGTTCC TATGTAGCTC TCACTGTCCA GGGACGCCCA
    CCTGGGTCGC CCCAGATCCC ACTTGCTGAT TCTGAAGTAG AGCCATCAGT CACTGGACAT
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82921
    Clone ID Related Accession (Same CDS sequence) XM_017313566.2 , XM_017313566.1
    Accession Version XM_017313566.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313566.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTCAGGAGCC GCAGTCGCTG
    GTCGGCAGTC CCTCCACCCG GGGCGCCCCT CACATCATTG GAGCAGAGGA TGATGATTTT
    GGTACTGAGC ATGAGCAGAT CAATGGACAG TGCAGCTGTT TCCAGAGCAT TGAATTGCTG
    AAGTCTCGCC CTGCTCACTT GGCTGTTTTC TTACACCATG TAGTCTCACA GTTTGACCCT
    GCAACATTGC TCTGTTATCT CTATTCAGAC CTGTATAAGC AGACAAACTC CAAGGAGACT
    CGGCGTGTCT TCCTGGAGTT TCATCAGTTC TTCTTGGACC GGTCTGCACA CCTGAAGGTT
    CCAGTTCCTG AGGAAATCTC CGTGGATCTA GAAAAGAGAA GACCTGAGCT TATTCCTGAG
    GATCTGCATC GCCTCTACAT TCAGACAATG CAGGAGAGAG TCCACCCTGA AGTTCAGAGG
    CACTTGGAGG ATTTCCGACA GAAACGCAGC ATGGGGCTGA CCTTGGCTGA GAGTGAGCTG
    ACTAAACTTG ATGCAGAGAG AGACAAGGAC CGGGGAACTC TGGAGAAGGA GCGAGCATGT
    GCGGAACAGA TCGTTACCAA AATTGAAGAA GTGTTGATGA CTGCTCAGGC TGTAGAAGAA
    GAAAGGAGTT CCACAATGCA GTATGTAATT CTCATGTATA TGAAGTATTT GGGAGTAAAA
    GTGAAAGAAC CTCGAAACTT GGAGCACAAG CGGGGTCGGA TCGGCTTCCT TCCAAAGATC
    AAGCAAAGCA TGAAGAAAGA TAGAGAGGGC GAAGAAAAAG GGAAGCGAAG AGGATTCCCC
    AGCATTTTGG GACCCCCAAG AAGACCAAGC CGCCACGACA ACAGTGCAAT TGGCAGGGCC
    ATGGAGATAC AGAAGTCACG CCACCCGAAG CACTTATCCA CTCCCTCGTC TGTGAGTCCT
    GAGCCTCAGG ACCCTGCCAA GTTGCGCCAG AGTGGAGTAG CCAATGAAGG AACAGACACT
    GGATACCTGC CCGCCAGTTC CATGTCCTCT GCCACCTCAG GGACTGCTCT TTCCCAAGAG
    GGAGGGAGAG AGAATGATAC AGGAACAAAA CAAGTGGGAG AAGCATCGGC GCCTGGAGAC
    TGCTTGGACA GCACACCTCG AGTCCCCACT ACTGTTTTCG ACTTCCCTCC TCCTCTATTA
    GATCAAGTGC AGGAGGAGGA GTGTGAAGTA GAGAGGGTGG CAGAACATGG GACCCCAAAA
    CCCTTTCGGA AGTTTGACAG CATCGCCTTT GGAGAAAGTC AGAGTGAGGA TGAGCAGTTT
    GAAAATGACC TGGAGACAGA TCCACCCAAC TGGCAGCAGC TTGTTAGCAG AGAAGTGTTA
    CTGGGACTGA AACCTTCTGA GATCAAAAGA CAGGAAGTGA TTAATGAATT GTTCTACACT
    GAGAGAGCTC ATGTCCGGAC ACTGAAGGTT CTTGACCAAG TATTCTATCA GCGAGTGTCC
    AGAGAAGGGA TTCTATCACC TTCAGAACTA CGCAAGATTT TTTCAAACTT GGAAGATATC
    CTTCAACTTC ATGTTGGGTT GAATGAGCAA ATGAAAGCTG TCCGAAAAAG GAATGAAACT
    TCTGTTATTG ATCATATTGG GGAAGATCTG CTCATCTGGT TTAGTGGACC AGGAGAGGAG
    AAGCTGAAAC ATGCTGCTGC CACATTCTGC AGTAACCAAC CTTTCGCCCT GGAAATGATC
    AAGTCTCGCC AGAAAAAGGA TTCTCGATTC CATACTTTTG TGCAAGACGC TGAGAGTAAC
    CCCCTGTGCC GTCGTCTTCA GCTGAAGGAC ATCATTCCCA CCCAAATGCA GAGACTCACT
    AAGTACCCGC TTCTTTTGGA TAACATTGCC AAGTACACAG AATGGCCTCC AGAAAGGGAA
    AAGGTGAAGA AGGCTGCAGA CCATTGTCGT CAGATCTTAA ATTATGTAAA TCAGGCTGTC
    AGGGAGGCAG AAAACAAGCA GCGCTTAGAA GACTATCAGC GTCGCCTTGA CACCTCCAAT
    CTCAAGTTGT CCGAGTACCC AAATGTTGAC GAGCTCAGGA ATTTGGATTT AACAAAAAGA
    AAGATGATTC ATGAAGGGCC GTTGGTCTGG AAGGTGAATA GAGATAAAAG CATCGATCTT
    TATACACTGC TGCTGGAAGA CATCCTTGTG CTGCTACAGA AGCAGGATGA CAGGCTGGTG
    CTAAGGTGCC ACAGCAAGAT TCTGGCATCC ACAGCCGACA GCAAACACAC CTTCAGCCCT
    GTCATTAAGC TGAGCACGGT GCTGGTTCGG CAGGTGGCGA CAGATAACAA AGCTTTATTC
    GTCATCTCCA TGTCAGATAA TGGAGCCCAA ATTTATGAAT TGGTAGCACA GACGGTTTCT
    GAAAAGACAG TCTGGCAAGA CCTCATCTGT CGAATGGCTG CGTCAGTGAA GGAGCAGTCT
    ACAAAGCCGA TTCCCCTCCC ACAGCCGCCA CCTTGCGAAG GAGACAATGA TGAAGAAGAA
    CCAGCCAAGT TAAAAGTGGA GCACCATGAT CTTTCAGTCG CTGGTCTGCA AAGTCCAGAC
    AGAGTTTTGG GATTAGAATC TCCCTTAATT TCGTCAAAGC CTCAGTCTCA TTCACTGAAT
    ACCCCTGGCA AATCAGCGGC AGAACATCTC TTTGTGACCG CAACACAGTT TGCAAAGGAA
    CAGCATGCAA ATGGGGCACT CAAGGAAGGT GATGGCGGGT ATCCAGTCAC AATCCCAGGT
    CCACACTTGC CTGTGTCAGA GGAGCGGTGG GCATTGGACG CACTGAGAAA TTTGGGCTTG
    TTGAAGCAGT TGCTGGTACA GCAGTTAGGT CTCACCGAGA AGAGTACTCA GGAAGACTGG
    CAGTCCTTCT CACGATACGG GCCAGCTTCT GAAGAGGTAC AGGCAGACAG TGGCATCCGG
    GACTTGGAAA ATGTTAAGGC TTGTCATGCC AGGGAAGGAC AGATGTCTTT TAAAACTGGA
    ACTGGTGATA TTGCAACTTG TGACAGTCCA CGGACTTCAA CTGAGTCTTG TGCTGCACAG
    GATTCAGTGA TACTGGCATC CCAAGACAGC CAGGCAAGTA ACGTTTTAGT AATGGACCAC
    ATGATCCTGA CCCCAGAGAT GCCTCCTGCA GAGCCAGAAG GGGGTCTGGA TGAGAGTGGA
    GAGCACTTTT TTGATGCCCG TGAAGCACAT AGTGATGATA ATCCATCAGA AGGTGATGGA
    GCAGTTAAAA AGGAAGAGAA GGATGTTAAT TTACGCATCT CAGGAAACTG TTTGATCCTT
    GATGGCTATG ATGCAGTTCA GGAGAGCTCC ACAGATGAGG AGGTTGCCTC CTCGTTCCCC
    CTGCAGCCTG TGACGGGCAT CCCCGCTGTG GACTCCAGCC ACCAGCAGCA ACATTCCCCT
    CAGAATGTGC ATCCCGAGGG GCCGGTTTCA CCATTCACCC CAGAATTCCT GGTCCAGCGG
    CACTGGCGAG CTATGGAGGA TACCTGTTTT GAGATCCAGA GTCCCTCATG TACAGATTCA
    CAAAGCCAAA TCCTGGAGTA CATTCATAAA ATAGAGGCTG ACCTGGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACGCTCT TCTTTGCCAA AGGCTGGCTG GCTCAGCCCT CCCCGACAAG
    CTCTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169055.1
    CDS45..4622
    Translation

    Target ORF information:

    RefSeq Version XM_017313566.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313566.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTCAGGAGCC GCAGTCGCTG
    GTCGGCAGTC CCTCCACCCG GGGCGCCCCT CACATCATTG GAGCAGAGGA TGATGATTTT
    GGTACTGAGC ATGAGCAGAT CAATGGACAG TGCAGCTGTT TCCAGAGCAT TGAATTGCTG
    AAGTCTCGCC CTGCTCACTT GGCTGTTTTC TTACACCATG TAGTCTCACA GTTTGACCCT
    GCAACATTGC TCTGTTATCT CTATTCAGAC CTGTATAAGC AGACAAACTC CAAGGAGACT
    CGGCGTGTCT TCCTGGAGTT TCATCAGTTC TTCTTGGACC GGTCTGCACA CCTGAAGGTT
    CCAGTTCCTG AGGAAATCTC CGTGGATCTA GAAAAGAGAA GACCTGAGCT TATTCCTGAG
    GATCTGCATC GCCTCTACAT TCAGACAATG CAGGAGAGAG TCCACCCTGA AGTTCAGAGG
    CACTTGGAGG ATTTCCGACA GAAACGCAGC ATGGGGCTGA CCTTGGCTGA GAGTGAGCTG
    ACTAAACTTG ATGCAGAGAG AGACAAGGAC CGGGGAACTC TGGAGAAGGA GCGAGCATGT
    GCGGAACAGA TCGTTACCAA AATTGAAGAA GTGTTGATGA CTGCTCAGGC TGTAGAAGAA
    GAAAGGAGTT CCACAATGCA GTATGTAATT CTCATGTATA TGAAGTATTT GGGAGTAAAA
    GTGAAAGAAC CTCGAAACTT GGAGCACAAG CGGGGTCGGA TCGGCTTCCT TCCAAAGATC
    AAGCAAAGCA TGAAGAAAGA TAGAGAGGGC GAAGAAAAAG GGAAGCGAAG AGGATTCCCC
    AGCATTTTGG GACCCCCAAG AAGACCAAGC CGCCACGACA ACAGTGCAAT TGGCAGGGCC
    ATGGAGATAC AGAAGTCACG CCACCCGAAG CACTTATCCA CTCCCTCGTC TGTGAGTCCT
    GAGCCTCAGG ACCCTGCCAA GTTGCGCCAG AGTGGAGTAG CCAATGAAGG AACAGACACT
    GGATACCTGC CCGCCAGTTC CATGTCCTCT GCCACCTCAG GGACTGCTCT TTCCCAAGAG
    GGAGGGAGAG AGAATGATAC AGGAACAAAA CAAGTGGGAG AAGCATCGGC GCCTGGAGAC
    TGCTTGGACA GCACACCTCG AGTCCCCACT ACTGTTTTCG ACTTCCCTCC TCCTCTATTA
    GATCAAGTGC AGGAGGAGGA GTGTGAAGTA GAGAGGGTGG CAGAACATGG GACCCCAAAA
    CCCTTTCGGA AGTTTGACAG CATCGCCTTT GGAGAAAGTC AGAGTGAGGA TGAGCAGTTT
    GAAAATGACC TGGAGACAGA TCCACCCAAC TGGCAGCAGC TTGTTAGCAG AGAAGTGTTA
    CTGGGACTGA AACCTTCTGA GATCAAAAGA CAGGAAGTGA TTAATGAATT GTTCTACACT
    GAGAGAGCTC ATGTCCGGAC ACTGAAGGTT CTTGACCAAG TATTCTATCA GCGAGTGTCC
    AGAGAAGGGA TTCTATCACC TTCAGAACTA CGCAAGATTT TTTCAAACTT GGAAGATATC
    CTTCAACTTC ATGTTGGGTT GAATGAGCAA ATGAAAGCTG TCCGAAAAAG GAATGAAACT
    TCTGTTATTG ATCATATTGG GGAAGATCTG CTCATCTGGT TTAGTGGACC AGGAGAGGAG
    AAGCTGAAAC ATGCTGCTGC CACATTCTGC AGTAACCAAC CTTTCGCCCT GGAAATGATC
    AAGTCTCGCC AGAAAAAGGA TTCTCGATTC CATACTTTTG TGCAAGACGC TGAGAGTAAC
    CCCCTGTGCC GTCGTCTTCA GCTGAAGGAC ATCATTCCCA CCCAAATGCA GAGACTCACT
    AAGTACCCGC TTCTTTTGGA TAACATTGCC AAGTACACAG AATGGCCTCC AGAAAGGGAA
    AAGGTGAAGA AGGCTGCAGA CCATTGTCGT CAGATCTTAA ATTATGTAAA TCAGGCTGTC
    AGGGAGGCAG AAAACAAGCA GCGCTTAGAA GACTATCAGC GTCGCCTTGA CACCTCCAAT
    CTCAAGTTGT CCGAGTACCC AAATGTTGAC GAGCTCAGGA ATTTGGATTT AACAAAAAGA
    AAGATGATTC ATGAAGGGCC GTTGGTCTGG AAGGTGAATA GAGATAAAAG CATCGATCTT
    TATACACTGC TGCTGGAAGA CATCCTTGTG CTGCTACAGA AGCAGGATGA CAGGCTGGTG
    CTAAGGTGCC ACAGCAAGAT TCTGGCATCC ACAGCCGACA GCAAACACAC CTTCAGCCCT
    GTCATTAAGC TGAGCACGGT GCTGGTTCGG CAGGTGGCGA CAGATAACAA AGCTTTATTC
    GTCATCTCCA TGTCAGATAA TGGAGCCCAA ATTTATGAAT TGGTAGCACA GACGGTTTCT
    GAAAAGACAG TCTGGCAAGA CCTCATCTGT CGAATGGCTG CGTCAGTGAA GGAGCAGTCT
    ACAAAGCCGA TTCCCCTCCC ACAGCCGCCA CCTTGCGAAG GAGACAATGA TGAAGAAGAA
    CCAGCCAAGT TAAAAGTGGA GCACCATGAT CTTTCAGTCG CTGGTCTGCA AAGTCCAGAC
    AGAGTTTTGG GATTAGAATC TCCCTTAATT TCGTCAAAGC CTCAGTCTCA TTCACTGAAT
    ACCCCTGGCA AATCAGCGGC AGAACATCTC TTTGTGACCG CAACACAGTT TGCAAAGGAA
    CAGCATGCAA ATGGGGCACT CAAGGAAGGT GATGGCGGGT ATCCAGTCAC AATCCCAGGT
    CCACACTTGC CTGTGTCAGA GGAGCGGTGG GCATTGGACG CACTGAGAAA TTTGGGCTTG
    TTGAAGCAGT TGCTGGTACA GCAGTTAGGT CTCACCGAGA AGAGTACTCA GGAAGACTGG
    CAGTCCTTCT CACGATACGG GCCAGCTTCT GAAGAGGTAC AGGCAGACAG TGGCATCCGG
    GACTTGGAAA ATGTTAAGGC TTGTCATGCC AGGGAAGGAC AGATGTCTTT TAAAACTGGA
    ACTGGTGATA TTGCAACTTG TGACAGTCCA CGGACTTCAA CTGAGTCTTG TGCTGCACAG
    GATTCAGTGA TACTGGCATC CCAAGACAGC CAGGCAAGTA ACGTTTTAGT AATGGACCAC
    ATGATCCTGA CCCCAGAGAT GCCTCCTGCA GAGCCAGAAG GGGGTCTGGA TGAGAGTGGA
    GAGCACTTTT TTGATGCCCG TGAAGCACAT AGTGATGATA ATCCATCAGA AGGTGATGGA
    GCAGTTAAAA AGGAAGAGAA GGATGTTAAT TTACGCATCT CAGGAAACTG TTTGATCCTT
    GATGGCTATG ATGCAGTTCA GGAGAGCTCC ACAGATGAGG AGGTTGCCTC CTCGTTCCCC
    CTGCAGCCTG TGACGGGCAT CCCCGCTGTG GACTCCAGCC ACCAGCAGCA ACATTCCCCT
    CAGAATGTGC ATCCCGAGGG GCCGGTTTCA CCATTCACCC CAGAATTCCT GGTCCAGCGG
    CACTGGCGAG CTATGGAGGA TACCTGTTTT GAGATCCAGA GTCCCTCATG TACAGATTCA
    CAAAGCCAAA TCCTGGAGTA CATTCATAAA ATAGAGGCTG ACCTGGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACGCTCT TCTTTGCCAA AGGCTGGCTG GCTCAGCCCT CCCCGACAAG
    CTCTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu82921
    Clone ID Related Accession (Same CDS sequence) XM_017313566.2 , XM_017313566.1
    Accession Version XM_017313566.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4578bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTCAGGAGCC GCAGTCGCTG
    GTCGGCAGTC CCTCCACCCG GGGCGCCCCT CACATCATTG GAGCAGAGGA TGATGATTTT
    GGTACTGAGC ATGAGCAGAT CAATGGACAG TGCAGCTGTT TCCAGAGCAT TGAATTGCTG
    AAGTCTCGCC CTGCTCACTT GGCTGTTTTC TTACACCATG TAGTCTCACA GTTTGACCCT
    GCAACATTGC TCTGTTATCT CTATTCAGAC CTGTATAAGC AGACAAACTC CAAGGAGACT
    CGGCGTGTCT TCCTGGAGTT TCATCAGTTC TTCTTGGACC GGTCTGCACA CCTGAAGGTT
    CCAGTTCCTG AGGAAATCTC CGTGGATCTA GAAAAGAGAA GACCTGAGCT TATTCCTGAG
    GATCTGCATC GCCTCTACAT TCAGACAATG CAGGAGAGAG TCCACCCTGA AGTTCAGAGG
    CACTTGGAGG ATTTCCGACA GAAACGCAGC ATGGGGCTGA CCTTGGCTGA GAGTGAGCTG
    ACTAAACTTG ATGCAGAGAG AGACAAGGAC CGGGGAACTC TGGAGAAGGA GCGAGCATGT
    GCGGAACAGA TCGTTACCAA AATTGAAGAA GTGTTGATGA CTGCTCAGGC TGTAGAAGAA
    GAAAGGAGTT CCACAATGCA GTATGTAATT CTCATGTATA TGAAGTATTT GGGAGTAAAA
    GTGAAAGAAC CTCGAAACTT GGAGCACAAG CGGGGTCGGA TCGGCTTCCT TCCAAAGATC
    AAGCAAAGCA TGAAGAAAGA TAGAGAGGGC GAAGAAAAAG GGAAGCGAAG AGGATTCCCC
    AGCATTTTGG GACCCCCAAG AAGACCAAGC CGCCACGACA ACAGTGCAAT TGGCAGGGCC
    ATGGAGATAC AGAAGTCACG CCACCCGAAG CACTTATCCA CTCCCTCGTC TGTGAGTCCT
    GAGCCTCAGG ACCCTGCCAA GTTGCGCCAG AGTGGAGTAG CCAATGAAGG AACAGACACT
    GGATACCTGC CCGCCAGTTC CATGTCCTCT GCCACCTCAG GGACTGCTCT TTCCCAAGAG
    GGAGGGAGAG AGAATGATAC AGGAACAAAA CAAGTGGGAG AAGCATCGGC GCCTGGAGAC
    TGCTTGGACA GCACACCTCG AGTCCCCACT ACTGTTTTCG ACTTCCCTCC TCCTCTATTA
    GATCAAGTGC AGGAGGAGGA GTGTGAAGTA GAGAGGGTGG CAGAACATGG GACCCCAAAA
    CCCTTTCGGA AGTTTGACAG CATCGCCTTT GGAGAAAGTC AGAGTGAGGA TGAGCAGTTT
    GAAAATGACC TGGAGACAGA TCCACCCAAC TGGCAGCAGC TTGTTAGCAG AGAAGTGTTA
    CTGGGACTGA AACCTTCTGA GATCAAAAGA CAGGAAGTGA TTAATGAATT GTTCTACACT
    GAGAGAGCTC ATGTCCGGAC ACTGAAGGTT CTTGACCAAG TATTCTATCA GCGAGTGTCC
    AGAGAAGGGA TTCTATCACC TTCAGAACTA CGCAAGATTT TTTCAAACTT GGAAGATATC
    CTTCAACTTC ATGTTGGGTT GAATGAGCAA ATGAAAGCTG TCCGAAAAAG GAATGAAACT
    TCTGTTATTG ATCATATTGG GGAAGATCTG CTCATCTGGT TTAGTGGACC AGGAGAGGAG
    AAGCTGAAAC ATGCTGCTGC CACATTCTGC AGTAACCAAC CTTTCGCCCT GGAAATGATC
    AAGTCTCGCC AGAAAAAGGA TTCTCGATTC CATACTTTTG TGCAAGACGC TGAGAGTAAC
    CCCCTGTGCC GTCGTCTTCA GCTGAAGGAC ATCATTCCCA CCCAAATGCA GAGACTCACT
    AAGTACCCGC TTCTTTTGGA TAACATTGCC AAGTACACAG AATGGCCTCC AGAAAGGGAA
    AAGGTGAAGA AGGCTGCAGA CCATTGTCGT CAGATCTTAA ATTATGTAAA TCAGGCTGTC
    AGGGAGGCAG AAAACAAGCA GCGCTTAGAA GACTATCAGC GTCGCCTTGA CACCTCCAAT
    CTCAAGTTGT CCGAGTACCC AAATGTTGAC GAGCTCAGGA ATTTGGATTT AACAAAAAGA
    AAGATGATTC ATGAAGGGCC GTTGGTCTGG AAGGTGAATA GAGATAAAAG CATCGATCTT
    TATACACTGC TGCTGGAAGA CATCCTTGTG CTGCTACAGA AGCAGGATGA CAGGCTGGTG
    CTAAGGTGCC ACAGCAAGAT TCTGGCATCC ACAGCCGACA GCAAACACAC CTTCAGCCCT
    GTCATTAAGC TGAGCACGGT GCTGGTTCGG CAGGTGGCGA CAGATAACAA AGCTTTATTC
    GTCATCTCCA TGTCAGATAA TGGAGCCCAA ATTTATGAAT TGGTAGCACA GACGGTTTCT
    GAAAAGACAG TCTGGCAAGA CCTCATCTGT CGAATGGCTG CGTCAGTGAA GGAGCAGTCT
    ACAAAGCCGA TTCCCCTCCC ACAGCCGCCA CCTTGCGAAG GAGACAATGA TGAAGAAGAA
    CCAGCCAAGT TAAAAGTGGA GCACCATGAT CTTTCAGTCG CTGGTCTGCA AAGTCCAGAC
    AGAGTTTTGG GATTAGAATC TCCCTTAATT TCGTCAAAGC CTCAGTCTCA TTCACTGAAT
    ACCCCTGGCA AATCAGCGGC AGAACATCTC TTTGTGACCG CAACACAGTT TGCAAAGGAA
    CAGCATGCAA ATGGGGCACT CAAGGAAGGT GATGGCGGGT ATCCAGTCAC AATCCCAGGT
    CCACACTTGC CTGTGTCAGA GGAGCGGTGG GCATTGGACG CACTGAGAAA TTTGGGCTTG
    TTGAAGCAGT TGCTGGTACA GCAGTTAGGT CTCACCGAGA AGAGTACTCA GGAAGACTGG
    CAGTCCTTCT CACGATACGG GCCAGCTTCT GAAGAGGTAC AGGCAGACAG TGGCATCCGG
    GACTTGGAAA ATGTTAAGGC TTGTCATGCC AGGGAAGGAC AGATGTCTTT TAAAACTGGA
    ACTGGTGATA TTGCAACTTG TGACAGTCCA CGGACTTCAA CTGAGTCTTG TGCTGCACAG
    GATTCAGTGA TACTGGCATC CCAAGACAGC CAGGCAAGTA ACGTTTTAGT AATGGACCAC
    ATGATCCTGA CCCCAGAGAT GCCTCCTGCA GAGCCAGAAG GGGGTCTGGA TGAGAGTGGA
    GAGCACTTTT TTGATGCCCG TGAAGCACAT AGTGATGATA ATCCATCAGA AGGTGATGGA
    GCAGTTAAAA AGGAAGAGAA GGATGTTAAT TTACGCATCT CAGGAAACTG TTTGATCCTT
    GATGGCTATG ATGCAGTTCA GGAGAGCTCC ACAGATGAGG AGGTTGCCTC CTCGTTCCCC
    CTGCAGCCTG TGACGGGCAT CCCCGCTGTG GACTCCAGCC ACCAGCAGCA ACATTCCCCT
    CAGAATGTGC ATCCCGAGGG GCCGGTTTCA CCATTCACCC CAGAATTCCT GGTCCAGCGG
    CACTGGCGAG CTATGGAGGA TACCTGTTTT GAGATCCAGA GTCCCTCATG TACAGATTCA
    CAAAGCCAAA TCCTGGAGTA CATTCATAAA ATAGAGGCTG ACCTGGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACGCTCT TCTTTGCCAA AGGCTGGCTG GCTCAGCCCT CCCCGACAAG
    CTCTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169055.1
    CDS45..4622
    Translation

    Target ORF information:

    RefSeq Version XM_017313566.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTCAGGAGCC GCAGTCGCTG
    GTCGGCAGTC CCTCCACCCG GGGCGCCCCT CACATCATTG GAGCAGAGGA TGATGATTTT
    GGTACTGAGC ATGAGCAGAT CAATGGACAG TGCAGCTGTT TCCAGAGCAT TGAATTGCTG
    AAGTCTCGCC CTGCTCACTT GGCTGTTTTC TTACACCATG TAGTCTCACA GTTTGACCCT
    GCAACATTGC TCTGTTATCT CTATTCAGAC CTGTATAAGC AGACAAACTC CAAGGAGACT
    CGGCGTGTCT TCCTGGAGTT TCATCAGTTC TTCTTGGACC GGTCTGCACA CCTGAAGGTT
    CCAGTTCCTG AGGAAATCTC CGTGGATCTA GAAAAGAGAA GACCTGAGCT TATTCCTGAG
    GATCTGCATC GCCTCTACAT TCAGACAATG CAGGAGAGAG TCCACCCTGA AGTTCAGAGG
    CACTTGGAGG ATTTCCGACA GAAACGCAGC ATGGGGCTGA CCTTGGCTGA GAGTGAGCTG
    ACTAAACTTG ATGCAGAGAG AGACAAGGAC CGGGGAACTC TGGAGAAGGA GCGAGCATGT
    GCGGAACAGA TCGTTACCAA AATTGAAGAA GTGTTGATGA CTGCTCAGGC TGTAGAAGAA
    GAAAGGAGTT CCACAATGCA GTATGTAATT CTCATGTATA TGAAGTATTT GGGAGTAAAA
    GTGAAAGAAC CTCGAAACTT GGAGCACAAG CGGGGTCGGA TCGGCTTCCT TCCAAAGATC
    AAGCAAAGCA TGAAGAAAGA TAGAGAGGGC GAAGAAAAAG GGAAGCGAAG AGGATTCCCC
    AGCATTTTGG GACCCCCAAG AAGACCAAGC CGCCACGACA ACAGTGCAAT TGGCAGGGCC
    ATGGAGATAC AGAAGTCACG CCACCCGAAG CACTTATCCA CTCCCTCGTC TGTGAGTCCT
    GAGCCTCAGG ACCCTGCCAA GTTGCGCCAG AGTGGAGTAG CCAATGAAGG AACAGACACT
    GGATACCTGC CCGCCAGTTC CATGTCCTCT GCCACCTCAG GGACTGCTCT TTCCCAAGAG
    GGAGGGAGAG AGAATGATAC AGGAACAAAA CAAGTGGGAG AAGCATCGGC GCCTGGAGAC
    TGCTTGGACA GCACACCTCG AGTCCCCACT ACTGTTTTCG ACTTCCCTCC TCCTCTATTA
    GATCAAGTGC AGGAGGAGGA GTGTGAAGTA GAGAGGGTGG CAGAACATGG GACCCCAAAA
    CCCTTTCGGA AGTTTGACAG CATCGCCTTT GGAGAAAGTC AGAGTGAGGA TGAGCAGTTT
    GAAAATGACC TGGAGACAGA TCCACCCAAC TGGCAGCAGC TTGTTAGCAG AGAAGTGTTA
    CTGGGACTGA AACCTTCTGA GATCAAAAGA CAGGAAGTGA TTAATGAATT GTTCTACACT
    GAGAGAGCTC ATGTCCGGAC ACTGAAGGTT CTTGACCAAG TATTCTATCA GCGAGTGTCC
    AGAGAAGGGA TTCTATCACC TTCAGAACTA CGCAAGATTT TTTCAAACTT GGAAGATATC
    CTTCAACTTC ATGTTGGGTT GAATGAGCAA ATGAAAGCTG TCCGAAAAAG GAATGAAACT
    TCTGTTATTG ATCATATTGG GGAAGATCTG CTCATCTGGT TTAGTGGACC AGGAGAGGAG
    AAGCTGAAAC ATGCTGCTGC CACATTCTGC AGTAACCAAC CTTTCGCCCT GGAAATGATC
    AAGTCTCGCC AGAAAAAGGA TTCTCGATTC CATACTTTTG TGCAAGACGC TGAGAGTAAC
    CCCCTGTGCC GTCGTCTTCA GCTGAAGGAC ATCATTCCCA CCCAAATGCA GAGACTCACT
    AAGTACCCGC TTCTTTTGGA TAACATTGCC AAGTACACAG AATGGCCTCC AGAAAGGGAA
    AAGGTGAAGA AGGCTGCAGA CCATTGTCGT CAGATCTTAA ATTATGTAAA TCAGGCTGTC
    AGGGAGGCAG AAAACAAGCA GCGCTTAGAA GACTATCAGC GTCGCCTTGA CACCTCCAAT
    CTCAAGTTGT CCGAGTACCC AAATGTTGAC GAGCTCAGGA ATTTGGATTT AACAAAAAGA
    AAGATGATTC ATGAAGGGCC GTTGGTCTGG AAGGTGAATA GAGATAAAAG CATCGATCTT
    TATACACTGC TGCTGGAAGA CATCCTTGTG CTGCTACAGA AGCAGGATGA CAGGCTGGTG
    CTAAGGTGCC ACAGCAAGAT TCTGGCATCC ACAGCCGACA GCAAACACAC CTTCAGCCCT
    GTCATTAAGC TGAGCACGGT GCTGGTTCGG CAGGTGGCGA CAGATAACAA AGCTTTATTC
    GTCATCTCCA TGTCAGATAA TGGAGCCCAA ATTTATGAAT TGGTAGCACA GACGGTTTCT
    GAAAAGACAG TCTGGCAAGA CCTCATCTGT CGAATGGCTG CGTCAGTGAA GGAGCAGTCT
    ACAAAGCCGA TTCCCCTCCC ACAGCCGCCA CCTTGCGAAG GAGACAATGA TGAAGAAGAA
    CCAGCCAAGT TAAAAGTGGA GCACCATGAT CTTTCAGTCG CTGGTCTGCA AAGTCCAGAC
    AGAGTTTTGG GATTAGAATC TCCCTTAATT TCGTCAAAGC CTCAGTCTCA TTCACTGAAT
    ACCCCTGGCA AATCAGCGGC AGAACATCTC TTTGTGACCG CAACACAGTT TGCAAAGGAA
    CAGCATGCAA ATGGGGCACT CAAGGAAGGT GATGGCGGGT ATCCAGTCAC AATCCCAGGT
    CCACACTTGC CTGTGTCAGA GGAGCGGTGG GCATTGGACG CACTGAGAAA TTTGGGCTTG
    TTGAAGCAGT TGCTGGTACA GCAGTTAGGT CTCACCGAGA AGAGTACTCA GGAAGACTGG
    CAGTCCTTCT CACGATACGG GCCAGCTTCT GAAGAGGTAC AGGCAGACAG TGGCATCCGG
    GACTTGGAAA ATGTTAAGGC TTGTCATGCC AGGGAAGGAC AGATGTCTTT TAAAACTGGA
    ACTGGTGATA TTGCAACTTG TGACAGTCCA CGGACTTCAA CTGAGTCTTG TGCTGCACAG
    GATTCAGTGA TACTGGCATC CCAAGACAGC CAGGCAAGTA ACGTTTTAGT AATGGACCAC
    ATGATCCTGA CCCCAGAGAT GCCTCCTGCA GAGCCAGAAG GGGGTCTGGA TGAGAGTGGA
    GAGCACTTTT TTGATGCCCG TGAAGCACAT AGTGATGATA ATCCATCAGA AGGTGATGGA
    GCAGTTAAAA AGGAAGAGAA GGATGTTAAT TTACGCATCT CAGGAAACTG TTTGATCCTT
    GATGGCTATG ATGCAGTTCA GGAGAGCTCC ACAGATGAGG AGGTTGCCTC CTCGTTCCCC
    CTGCAGCCTG TGACGGGCAT CCCCGCTGTG GACTCCAGCC ACCAGCAGCA ACATTCCCCT
    CAGAATGTGC ATCCCGAGGG GCCGGTTTCA CCATTCACCC CAGAATTCCT GGTCCAGCGG
    CACTGGCGAG CTATGGAGGA TACCTGTTTT GAGATCCAGA GTCCCTCATG TACAGATTCA
    CAAAGCCAAA TCCTGGAGTA CATTCATAAA ATAGAGGCTG ACCTGGAACA CTTAAAGAAG
    GTGGAGGAAA GTTACGCTCT TCTTTGCCAA AGGCTGGCTG GCTCAGCCCT CCCCGACAAG
    CTCTCAGATA AAAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21950
    Clone ID Related Accession (Same CDS sequence) NM_027144.2
    Accession Version NM_027144.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-11
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110169.7 and AC160123.2. On Apr 12, 2007 this sequence version replaced NM_027144.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK122267.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTC AGGAGCCGCA GTCGCTGGTC
    GGCAGTCCCT CCACCCGGGG CGCCCCTCAC ATCATTGGAG CAGAGGATGA TGATTTTGGT
    ACTGAGCATG AGCAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTGCTGAAG
    TCTCGCCCTG CTCACTTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCTGCA
    ACATTGCTCT GTTATCTCTA TTCAGACCTG TATAAGCAGA CAAACTCCAA GGAGACTCGG
    CGTGTCTTCC TGGAGTTTCA TCAGTTCTTC TTGGACCGGT CTGCACACCT GAAGGTTCCA
    GTTCCTGAGG AAATCTCCGT GGATCTAGAA AAGAGAAGAC CTGAGCTTAT TCCTGAGGAT
    CTGCATCGCC TCTACATTCA GACAATGCAG GAGAGAGTCC ACCCTGAAGT TCAGAGGCAC
    TTGGAGGATT TCCGACAGAA ACGCAGCATG GGGCTGACCT TGGCTGAGAG TGAGCTGACT
    AAACTTGATG CAGAGAGAGA CAAGGACCGG GGAACTCTGG AGAAGGAGCG AGCATGTGCG
    GAACAGATCG TTACCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA
    AGGAGTTCCA CAATGCAGTA TGTAATTCTC ATGTATATGA AGTATTTGGG AGTAAAAGTG
    AAAGAACCTC GAAACTTGGA GCACAAGCGG GGTCGGATCG GCTTCCTTCC AAAGATCAAG
    CAAAGCATGA AGAAAGATAG AGAGGGCGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATTTTGGGAC CCCCAAGAAG ACCAAGCCGC CACGACAACA GTGCAATTGG CAGGGCCATG
    GAGATACAGA AGTCACGCCA CCCGAAGCAC TTATCCACTC CCTCGTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GGAGTAGCCA ATGAAGGAAC AGACACTGGA
    TACCTGCCCG CCAGTTCCAT GTCCTCTGCC ACCTCAGGGA CTGCTCTTTC CCAAGAGGGA
    GGGAGAGAGA ATGATACAGG AACAAAACAA GTGGGAGAAG CATCGGCGCC TGGAGACTGC
    TTGGACAGCA CACCTCGAGT CCCCACTACT GTTTTCGACT TCCCTCCTCC TCTATTAGAT
    CAAGTGCAGG AGGAGGAGTG TGAAGTAGAG AGGGTGGCAG AACATGGGAC CCCAAAACCC
    TTTCGGAAGT TTGACAGCAT CGCCTTTGGA GAAAGTCAGA GTGAGGATGA GCAGTTTGAA
    AATGACCTGG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGCAGAGA AGTGTTACTG
    GGACTGAAAC CTTCTGAGAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAG
    AGAGCTCATG TCCGGACACT GAAGGTTCTT GACCAAGTAT TCTATCAGCG AGTGTCCAGA
    GAAGGGATTC TATCACCTTC AGAACTACGC AAGATTTTTT CAAACTTGGA AGATATCCTT
    CAACTTCATG TTGGGTTGAA TGAGCAAATG AAAGCTGTCC GAAAAAGGAA TGAAACTTCT
    GTTATTGATC ATATTGGGGA AGATCTGCTC ATCTGGTTTA GTGGACCAGG AGAGGAGAAG
    CTGAAACATG CTGCTGCCAC ATTCTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG
    TCTCGCCAGA AAAAGGATTC TCGATTCCAT ACTTTTGTGC AAGACGCTGA GAGTAACCCC
    CTGTGCCGTC GTCTTCAGCT GAAGGACATC ATTCCCACCC AAATGCAGAG ACTCACTAAG
    TACCCGCTTC TTTTGGATAA CATTGCCAAG TACACAGAAT GGCCTCCAGA AAGGGAAAAG
    GTGAAGAAGG CTGCAGACCA TTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAGG
    GAGGCAGAAA ACAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTTGACAC CTCCAATCTC
    AAGTTGTCCG AGTACCCAAA TGTTGACGAG CTCAGGAATT TGGATTTAAC AAAAAGAAAG
    ATGATTCATG AAGGGCCGTT GGTCTGGAAG GTGAATAGAG ATAAAAGCAT CGATCTTTAT
    ACACTGCTGC TGGAAGACAT CCTTGTGCTG CTACAGAAGC AGGATGACAG GCTGGTGCTA
    AGGTGCCACA GCAAGATTCT GGCATCCACA GCCGACAGCA AACACACCTT CAGCCCTGTC
    ATTAAGCTGA GCACGGTGCT GGTTCGGCAG GTGGCGACAG ATAACAAAGC TTTATTCGTC
    ATCTCCATGT CAGATAATGG AGCCCAAATT TATGAATTGG TAGCACAGAC GGTTTCTGAA
    AAGACAGTCT GGCAAGACCT CATCTGTCGA ATGGCTGCGT CAGTGAAGGA GCAGTCTACA
    AAGCCGATTC CCCTCCCACA GCCGCCACCT TGCGAAGGAG ACAATGATGA AGAAGAACCA
    GCCAAGTTAA AAGTGGAGCA CCATGATCTT TCAGTCGCTG GTCTGCAAAG TCCAGACAGA
    GTTTTGGGAT TAGAATCTCC CTTAATTTCG TCAAAGCCTC AGTCTCATTC ACTGAATACC
    CCTGGCAAAT CAGCGGCAGA ACATCTCTTT GTGACCGCAA CACAGTTTGC AAAGGAACAG
    CATGCAAATG GGGCACTCAA GGAAGGTGAT GGCGGGTATC CAGTCACAAT CCCAGGTCCA
    CACTTGCCTG TGTCAGAGGA GCGGTGGGCA TTGGACGCAC TGAGAAATTT GGGCTTGTTG
    AAGCAGTTGC TGGTACAGCA GTTAGGTCTC ACCGAGAAGA GTACTCAGGA AGACTGGCAG
    TCCTTCTCAC GATACGGGCC AGCTTCTGAA GAGGTACAGG CAGACAGTGG CATCCGGGAC
    TTGGAAAATG TTAAGGCTTG TCATGCCAGG GAAGGACAGA TGTCTTTTAA AACTGGAACT
    GGTGATATTG CAACTTGTGA CAGTCCACGG ACTTCAACTG AGTCTTGTGC TGCACAGGAT
    TCAGTGATAC TGGCATCCCA AGACAGCCAG GCAAGTAACG TTTTAGTAAT GGACCACATG
    ATCCTGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGG GTCTGGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTGTTT GATCCTTGAT
    GGCTATGATG CAGTTCAGGA GAGCTCCACA GATGAGGAGG TTGCCTCCTC GTTCCCCCTG
    CAGCCTGTGA CGGGCATCCC CGCTGTGGAC TCCAGCCACC AGCAGCAACA TTCCCCTCAG
    AATGTGCATC CCGAGGGGCC GGTTTCACCA TTCACCCCAG AATTCCTGGT CCAGCGGCAC
    TGGCGAGCTA TGGAGGATAC CTGTTTTGAG ATCCAGAGTC CCTCATGTAC AGATTCACAA
    AGCCAAATCC TGGAGTACAT TCATAAAATA GAGGCTGACC TGGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACGCTCTTCT TTGCCAAAGG CTGGCTGGCT CAGCCCTCCC CGACAAGCTC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_081420.2
    CDS509..5143
    Translation

    Target ORF information:

    RefSeq Version NM_027144.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_027144.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTC AGGAGCCGCA GTCGCTGGTC
    GGCAGTCCCT CCACCCGGGG CGCCCCTCAC ATCATTGGAG CAGAGGATGA TGATTTTGGT
    ACTGAGCATG AGCAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTGCTGAAG
    TCTCGCCCTG CTCACTTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCTGCA
    ACATTGCTCT GTTATCTCTA TTCAGACCTG TATAAGCAGA CAAACTCCAA GGAGACTCGG
    CGTGTCTTCC TGGAGTTTCA TCAGTTCTTC TTGGACCGGT CTGCACACCT GAAGGTTCCA
    GTTCCTGAGG AAATCTCCGT GGATCTAGAA AAGAGAAGAC CTGAGCTTAT TCCTGAGGAT
    CTGCATCGCC TCTACATTCA GACAATGCAG GAGAGAGTCC ACCCTGAAGT TCAGAGGCAC
    TTGGAGGATT TCCGACAGAA ACGCAGCATG GGGCTGACCT TGGCTGAGAG TGAGCTGACT
    AAACTTGATG CAGAGAGAGA CAAGGACCGG GGAACTCTGG AGAAGGAGCG AGCATGTGCG
    GAACAGATCG TTACCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA
    AGGAGTTCCA CAATGCAGTA TGTAATTCTC ATGTATATGA AGTATTTGGG AGTAAAAGTG
    AAAGAACCTC GAAACTTGGA GCACAAGCGG GGTCGGATCG GCTTCCTTCC AAAGATCAAG
    CAAAGCATGA AGAAAGATAG AGAGGGCGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATTTTGGGAC CCCCAAGAAG ACCAAGCCGC CACGACAACA GTGCAATTGG CAGGGCCATG
    GAGATACAGA AGTCACGCCA CCCGAAGCAC TTATCCACTC CCTCGTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GGAGTAGCCA ATGAAGGAAC AGACACTGGA
    TACCTGCCCG CCAGTTCCAT GTCCTCTGCC ACCTCAGGGA CTGCTCTTTC CCAAGAGGGA
    GGGAGAGAGA ATGATACAGG AACAAAACAA GTGGGAGAAG CATCGGCGCC TGGAGACTGC
    TTGGACAGCA CACCTCGAGT CCCCACTACT GTTTTCGACT TCCCTCCTCC TCTATTAGAT
    CAAGTGCAGG AGGAGGAGTG TGAAGTAGAG AGGGTGGCAG AACATGGGAC CCCAAAACCC
    TTTCGGAAGT TTGACAGCAT CGCCTTTGGA GAAAGTCAGA GTGAGGATGA GCAGTTTGAA
    AATGACCTGG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGCAGAGA AGTGTTACTG
    GGACTGAAAC CTTCTGAGAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAG
    AGAGCTCATG TCCGGACACT GAAGGTTCTT GACCAAGTAT TCTATCAGCG AGTGTCCAGA
    GAAGGGATTC TATCACCTTC AGAACTACGC AAGATTTTTT CAAACTTGGA AGATATCCTT
    CAACTTCATG TTGGGTTGAA TGAGCAAATG AAAGCTGTCC GAAAAAGGAA TGAAACTTCT
    GTTATTGATC ATATTGGGGA AGATCTGCTC ATCTGGTTTA GTGGACCAGG AGAGGAGAAG
    CTGAAACATG CTGCTGCCAC ATTCTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG
    TCTCGCCAGA AAAAGGATTC TCGATTCCAT ACTTTTGTGC AAGACGCTGA GAGTAACCCC
    CTGTGCCGTC GTCTTCAGCT GAAGGACATC ATTCCCACCC AAATGCAGAG ACTCACTAAG
    TACCCGCTTC TTTTGGATAA CATTGCCAAG TACACAGAAT GGCCTCCAGA AAGGGAAAAG
    GTGAAGAAGG CTGCAGACCA TTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAGG
    GAGGCAGAAA ACAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTTGACAC CTCCAATCTC
    AAGTTGTCCG AGTACCCAAA TGTTGACGAG CTCAGGAATT TGGATTTAAC AAAAAGAAAG
    ATGATTCATG AAGGGCCGTT GGTCTGGAAG GTGAATAGAG ATAAAAGCAT CGATCTTTAT
    ACACTGCTGC TGGAAGACAT CCTTGTGCTG CTACAGAAGC AGGATGACAG GCTGGTGCTA
    AGGTGCCACA GCAAGATTCT GGCATCCACA GCCGACAGCA AACACACCTT CAGCCCTGTC
    ATTAAGCTGA GCACGGTGCT GGTTCGGCAG GTGGCGACAG ATAACAAAGC TTTATTCGTC
    ATCTCCATGT CAGATAATGG AGCCCAAATT TATGAATTGG TAGCACAGAC GGTTTCTGAA
    AAGACAGTCT GGCAAGACCT CATCTGTCGA ATGGCTGCGT CAGTGAAGGA GCAGTCTACA
    AAGCCGATTC CCCTCCCACA GCCGCCACCT TGCGAAGGAG ACAATGATGA AGAAGAACCA
    GCCAAGTTAA AAGTGGAGCA CCATGATCTT TCAGTCGCTG GTCTGCAAAG TCCAGACAGA
    GTTTTGGGAT TAGAATCTCC CTTAATTTCG TCAAAGCCTC AGTCTCATTC ACTGAATACC
    CCTGGCAAAT CAGCGGCAGA ACATCTCTTT GTGACCGCAA CACAGTTTGC AAAGGAACAG
    CATGCAAATG GGGCACTCAA GGAAGGTGAT GGCGGGTATC CAGTCACAAT CCCAGGTCCA
    CACTTGCCTG TGTCAGAGGA GCGGTGGGCA TTGGACGCAC TGAGAAATTT GGGCTTGTTG
    AAGCAGTTGC TGGTACAGCA GTTAGGTCTC ACCGAGAAGA GTACTCAGGA AGACTGGCAG
    TCCTTCTCAC GATACGGGCC AGCTTCTGAA GAGGTACAGG CAGACAGTGG CATCCGGGAC
    TTGGAAAATG TTAAGGCTTG TCATGCCAGG GAAGGACAGA TGTCTTTTAA AACTGGAACT
    GGTGATATTG CAACTTGTGA CAGTCCACGG ACTTCAACTG AGTCTTGTGC TGCACAGGAT
    TCAGTGATAC TGGCATCCCA AGACAGCCAG GCAAGTAACG TTTTAGTAAT GGACCACATG
    ATCCTGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGG GTCTGGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTGTTT GATCCTTGAT
    GGCTATGATG CAGTTCAGGA GAGCTCCACA GATGAGGAGG TTGCCTCCTC GTTCCCCCTG
    CAGCCTGTGA CGGGCATCCC CGCTGTGGAC TCCAGCCACC AGCAGCAACA TTCCCCTCAG
    AATGTGCATC CCGAGGGGCC GGTTTCACCA TTCACCCCAG AATTCCTGGT CCAGCGGCAC
    TGGCGAGCTA TGGAGGATAC CTGTTTTGAG ATCCAGAGTC CCTCATGTAC AGATTCACAA
    AGCCAAATCC TGGAGTACAT TCATAAAATA GAGGCTGACC TGGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACGCTCTTCT TTGCCAAAGG CTGGCTGGCT CAGCCCTCCC CGACAAGCTC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu42386
    Clone ID Related Accession (Same CDS sequence) XM_006510586.2 , NM_001359232.1 , XM_006510586.3
    Accession Version NM_001359232.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-11
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110169.7 and AC160123.2. On Dec 29, 2017 this sequence version replaced XM_006510586.3. Transcript Variant: This variant (2) lacks a three-nucleotide segment in the central coding region, compared to variant 1. The encoded protein (isoform 2) is shorter, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF467766.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTG AGCCGCAGTC GCTGGTCGGC
    AGTCCCTCCA CCCGGGGCGC CCCTCACATC ATTGGAGCAG AGGATGATGA TTTTGGTACT
    GAGCATGAGC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT GCTGAAGTCT
    CGCCCTGCTC ACTTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCTGCAACA
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAGCAGACAA ACTCCAAGGA GACTCGGCGT
    GTCTTCCTGG AGTTTCATCA GTTCTTCTTG GACCGGTCTG CACACCTGAA GGTTCCAGTT
    CCTGAGGAAA TCTCCGTGGA TCTAGAAAAG AGAAGACCTG AGCTTATTCC TGAGGATCTG
    CATCGCCTCT ACATTCAGAC AATGCAGGAG AGAGTCCACC CTGAAGTTCA GAGGCACTTG
    GAGGATTTCC GACAGAAACG CAGCATGGGG CTGACCTTGG CTGAGAGTGA GCTGACTAAA
    CTTGATGCAG AGAGAGACAA GGACCGGGGA ACTCTGGAGA AGGAGCGAGC ATGTGCGGAA
    CAGATCGTTA CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAGG
    AGTTCCACAA TGCAGTATGT AATTCTCATG TATATGAAGT ATTTGGGAGT AAAAGTGAAA
    GAACCTCGAA ACTTGGAGCA CAAGCGGGGT CGGATCGGCT TCCTTCCAAA GATCAAGCAA
    AGCATGAAGA AAGATAGAGA GGGCGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATT
    TTGGGACCCC CAAGAAGACC AAGCCGCCAC GACAACAGTG CAATTGGCAG GGCCATGGAG
    ATACAGAAGT CACGCCACCC GAAGCACTTA TCCACTCCCT CGTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGGA GTAGCCAATG AAGGAACAGA CACTGGATAC
    CTGCCCGCCA GTTCCATGTC CTCTGCCACC TCAGGGACTG CTCTTTCCCA AGAGGGAGGG
    AGAGAGAATG ATACAGGAAC AAAACAAGTG GGAGAAGCAT CGGCGCCTGG AGACTGCTTG
    GACAGCACAC CTCGAGTCCC CACTACTGTT TTCGACTTCC CTCCTCCTCT ATTAGATCAA
    GTGCAGGAGG AGGAGTGTGA AGTAGAGAGG GTGGCAGAAC ATGGGACCCC AAAACCCTTT
    CGGAAGTTTG ACAGCATCGC CTTTGGAGAA AGTCAGAGTG AGGATGAGCA GTTTGAAAAT
    GACCTGGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GCAGAGAAGT GTTACTGGGA
    CTGAAACCTT CTGAGATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAGAGA
    GCTCATGTCC GGACACTGAA GGTTCTTGAC CAAGTATTCT ATCAGCGAGT GTCCAGAGAA
    GGGATTCTAT CACCTTCAGA ACTACGCAAG ATTTTTTCAA ACTTGGAAGA TATCCTTCAA
    CTTCATGTTG GGTTGAATGA GCAAATGAAA GCTGTCCGAA AAAGGAATGA AACTTCTGTT
    ATTGATCATA TTGGGGAAGA TCTGCTCATC TGGTTTAGTG GACCAGGAGA GGAGAAGCTG
    AAACATGCTG CTGCCACATT CTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT
    CGCCAGAAAA AGGATTCTCG ATTCCATACT TTTGTGCAAG ACGCTGAGAG TAACCCCCTG
    TGCCGTCGTC TTCAGCTGAA GGACATCATT CCCACCCAAA TGCAGAGACT CACTAAGTAC
    CCGCTTCTTT TGGATAACAT TGCCAAGTAC ACAGAATGGC CTCCAGAAAG GGAAAAGGTG
    AAGAAGGCTG CAGACCATTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAGGGAG
    GCAGAAAACA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TTGACACCTC CAATCTCAAG
    TTGTCCGAGT ACCCAAATGT TGACGAGCTC AGGAATTTGG ATTTAACAAA AAGAAAGATG
    ATTCATGAAG GGCCGTTGGT CTGGAAGGTG AATAGAGATA AAAGCATCGA TCTTTATACA
    CTGCTGCTGG AAGACATCCT TGTGCTGCTA CAGAAGCAGG ATGACAGGCT GGTGCTAAGG
    TGCCACAGCA AGATTCTGGC ATCCACAGCC GACAGCAAAC ACACCTTCAG CCCTGTCATT
    AAGCTGAGCA CGGTGCTGGT TCGGCAGGTG GCGACAGATA ACAAAGCTTT ATTCGTCATC
    TCCATGTCAG ATAATGGAGC CCAAATTTAT GAATTGGTAG CACAGACGGT TTCTGAAAAG
    ACAGTCTGGC AAGACCTCAT CTGTCGAATG GCTGCGTCAG TGAAGGAGCA GTCTACAAAG
    CCGATTCCCC TCCCACAGCC GCCACCTTGC GAAGGAGACA ATGATGAAGA AGAACCAGCC
    AAGTTAAAAG TGGAGCACCA TGATCTTTCA GTCGCTGGTC TGCAAAGTCC AGACAGAGTT
    TTGGGATTAG AATCTCCCTT AATTTCGTCA AAGCCTCAGT CTCATTCACT GAATACCCCT
    GGCAAATCAG CGGCAGAACA TCTCTTTGTG ACCGCAACAC AGTTTGCAAA GGAACAGCAT
    GCAAATGGGG CACTCAAGGA AGGTGATGGC GGGTATCCAG TCACAATCCC AGGTCCACAC
    TTGCCTGTGT CAGAGGAGCG GTGGGCATTG GACGCACTGA GAAATTTGGG CTTGTTGAAG
    CAGTTGCTGG TACAGCAGTT AGGTCTCACC GAGAAGAGTA CTCAGGAAGA CTGGCAGTCC
    TTCTCACGAT ACGGGCCAGC TTCTGAAGAG GTACAGGCAG ACAGTGGCAT CCGGGACTTG
    GAAAATGTTA AGGCTTGTCA TGCCAGGGAA GGACAGATGT CTTTTAAAAC TGGAACTGGT
    GATATTGCAA CTTGTGACAG TCCACGGACT TCAACTGAGT CTTGTGCTGC ACAGGATTCA
    GTGATACTGG CATCCCAAGA CAGCCAGGCA AGTAACGTTT TAGTAATGGA CCACATGATC
    CTGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGGGTC TGGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTGTTTGAT CCTTGATGGC
    TATGATGCAG TTCAGGAGAG CTCCACAGAT GAGGAGGTTG CCTCCTCGTT CCCCCTGCAG
    CCTGTGACGG GCATCCCCGC TGTGGACTCC AGCCACCAGC AGCAACATTC CCCTCAGAAT
    GTGCATCCCG AGGGGCCGGT TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCGGCACTGG
    CGAGCTATGG AGGATACCTG TTTTGAGATC CAGAGTCCCT CATGTACAGA TTCACAAAGC
    CAAATCCTGG AGTACATTCA TAAAATAGAG GCTGACCTGG AACACTTAAA GAAGGTGGAG
    GAAAGTTACG CTCTTCTTTG CCAAAGGCTG GCTGGCTCAG CCCTCCCCGA CAAGCTCTCA
    GATAAAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001346161.1
    CDS509..5140
    Translation

    Target ORF information:

    RefSeq Version NM_001359232.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001359232.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTG AGCCGCAGTC GCTGGTCGGC
    AGTCCCTCCA CCCGGGGCGC CCCTCACATC ATTGGAGCAG AGGATGATGA TTTTGGTACT
    GAGCATGAGC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT GCTGAAGTCT
    CGCCCTGCTC ACTTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCTGCAACA
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAGCAGACAA ACTCCAAGGA GACTCGGCGT
    GTCTTCCTGG AGTTTCATCA GTTCTTCTTG GACCGGTCTG CACACCTGAA GGTTCCAGTT
    CCTGAGGAAA TCTCCGTGGA TCTAGAAAAG AGAAGACCTG AGCTTATTCC TGAGGATCTG
    CATCGCCTCT ACATTCAGAC AATGCAGGAG AGAGTCCACC CTGAAGTTCA GAGGCACTTG
    GAGGATTTCC GACAGAAACG CAGCATGGGG CTGACCTTGG CTGAGAGTGA GCTGACTAAA
    CTTGATGCAG AGAGAGACAA GGACCGGGGA ACTCTGGAGA AGGAGCGAGC ATGTGCGGAA
    CAGATCGTTA CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAGG
    AGTTCCACAA TGCAGTATGT AATTCTCATG TATATGAAGT ATTTGGGAGT AAAAGTGAAA
    GAACCTCGAA ACTTGGAGCA CAAGCGGGGT CGGATCGGCT TCCTTCCAAA GATCAAGCAA
    AGCATGAAGA AAGATAGAGA GGGCGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATT
    TTGGGACCCC CAAGAAGACC AAGCCGCCAC GACAACAGTG CAATTGGCAG GGCCATGGAG
    ATACAGAAGT CACGCCACCC GAAGCACTTA TCCACTCCCT CGTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGGA GTAGCCAATG AAGGAACAGA CACTGGATAC
    CTGCCCGCCA GTTCCATGTC CTCTGCCACC TCAGGGACTG CTCTTTCCCA AGAGGGAGGG
    AGAGAGAATG ATACAGGAAC AAAACAAGTG GGAGAAGCAT CGGCGCCTGG AGACTGCTTG
    GACAGCACAC CTCGAGTCCC CACTACTGTT TTCGACTTCC CTCCTCCTCT ATTAGATCAA
    GTGCAGGAGG AGGAGTGTGA AGTAGAGAGG GTGGCAGAAC ATGGGACCCC AAAACCCTTT
    CGGAAGTTTG ACAGCATCGC CTTTGGAGAA AGTCAGAGTG AGGATGAGCA GTTTGAAAAT
    GACCTGGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GCAGAGAAGT GTTACTGGGA
    CTGAAACCTT CTGAGATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAGAGA
    GCTCATGTCC GGACACTGAA GGTTCTTGAC CAAGTATTCT ATCAGCGAGT GTCCAGAGAA
    GGGATTCTAT CACCTTCAGA ACTACGCAAG ATTTTTTCAA ACTTGGAAGA TATCCTTCAA
    CTTCATGTTG GGTTGAATGA GCAAATGAAA GCTGTCCGAA AAAGGAATGA AACTTCTGTT
    ATTGATCATA TTGGGGAAGA TCTGCTCATC TGGTTTAGTG GACCAGGAGA GGAGAAGCTG
    AAACATGCTG CTGCCACATT CTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT
    CGCCAGAAAA AGGATTCTCG ATTCCATACT TTTGTGCAAG ACGCTGAGAG TAACCCCCTG
    TGCCGTCGTC TTCAGCTGAA GGACATCATT CCCACCCAAA TGCAGAGACT CACTAAGTAC
    CCGCTTCTTT TGGATAACAT TGCCAAGTAC ACAGAATGGC CTCCAGAAAG GGAAAAGGTG
    AAGAAGGCTG CAGACCATTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAGGGAG
    GCAGAAAACA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TTGACACCTC CAATCTCAAG
    TTGTCCGAGT ACCCAAATGT TGACGAGCTC AGGAATTTGG ATTTAACAAA AAGAAAGATG
    ATTCATGAAG GGCCGTTGGT CTGGAAGGTG AATAGAGATA AAAGCATCGA TCTTTATACA
    CTGCTGCTGG AAGACATCCT TGTGCTGCTA CAGAAGCAGG ATGACAGGCT GGTGCTAAGG
    TGCCACAGCA AGATTCTGGC ATCCACAGCC GACAGCAAAC ACACCTTCAG CCCTGTCATT
    AAGCTGAGCA CGGTGCTGGT TCGGCAGGTG GCGACAGATA ACAAAGCTTT ATTCGTCATC
    TCCATGTCAG ATAATGGAGC CCAAATTTAT GAATTGGTAG CACAGACGGT TTCTGAAAAG
    ACAGTCTGGC AAGACCTCAT CTGTCGAATG GCTGCGTCAG TGAAGGAGCA GTCTACAAAG
    CCGATTCCCC TCCCACAGCC GCCACCTTGC GAAGGAGACA ATGATGAAGA AGAACCAGCC
    AAGTTAAAAG TGGAGCACCA TGATCTTTCA GTCGCTGGTC TGCAAAGTCC AGACAGAGTT
    TTGGGATTAG AATCTCCCTT AATTTCGTCA AAGCCTCAGT CTCATTCACT GAATACCCCT
    GGCAAATCAG CGGCAGAACA TCTCTTTGTG ACCGCAACAC AGTTTGCAAA GGAACAGCAT
    GCAAATGGGG CACTCAAGGA AGGTGATGGC GGGTATCCAG TCACAATCCC AGGTCCACAC
    TTGCCTGTGT CAGAGGAGCG GTGGGCATTG GACGCACTGA GAAATTTGGG CTTGTTGAAG
    CAGTTGCTGG TACAGCAGTT AGGTCTCACC GAGAAGAGTA CTCAGGAAGA CTGGCAGTCC
    TTCTCACGAT ACGGGCCAGC TTCTGAAGAG GTACAGGCAG ACAGTGGCAT CCGGGACTTG
    GAAAATGTTA AGGCTTGTCA TGCCAGGGAA GGACAGATGT CTTTTAAAAC TGGAACTGGT
    GATATTGCAA CTTGTGACAG TCCACGGACT TCAACTGAGT CTTGTGCTGC ACAGGATTCA
    GTGATACTGG CATCCCAAGA CAGCCAGGCA AGTAACGTTT TAGTAATGGA CCACATGATC
    CTGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGGGTC TGGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTGTTTGAT CCTTGATGGC
    TATGATGCAG TTCAGGAGAG CTCCACAGAT GAGGAGGTTG CCTCCTCGTT CCCCCTGCAG
    CCTGTGACGG GCATCCCCGC TGTGGACTCC AGCCACCAGC AGCAACATTC CCCTCAGAAT
    GTGCATCCCG AGGGGCCGGT TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCGGCACTGG
    CGAGCTATGG AGGATACCTG TTTTGAGATC CAGAGTCCCT CATGTACAGA TTCACAAAGC
    CAAATCCTGG AGTACATTCA TAAAATAGAG GCTGACCTGG AACACTTAAA GAAGGTGGAG
    GAAAGTTACG CTCTTCTTTG CCAAAGGCTG GCTGGCTCAG CCCTCCCCGA CAAGCTCTCA
    GATAAAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu42386
    Clone ID Related Accession (Same CDS sequence) XM_006510586.2 , NM_001359232.1 , XM_006510586.3
    Accession Version XM_006510586.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-02-08
    Organism Mus musculus(House mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTG AGCCGCAGTC GCTGGTCGGC
    AGTCCCTCCA CCCGGGGCGC CCCTCACATC ATTGGAGCAG AGGATGATGA TTTTGGTACT
    GAGCATGAGC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT GCTGAAGTCT
    CGCCCTGCTC ACTTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCTGCAACA
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAGCAGACAA ACTCCAAGGA GACTCGGCGT
    GTCTTCCTGG AGTTTCATCA GTTCTTCTTG GACCGGTCTG CACACCTGAA GGTTCCAGTT
    CCTGAGGAAA TCTCCGTGGA TCTAGAAAAG AGAAGACCTG AGCTTATTCC TGAGGATCTG
    CATCGCCTCT ACATTCAGAC AATGCAGGAG AGAGTCCACC CTGAAGTTCA GAGGCACTTG
    GAGGATTTCC GACAGAAACG CAGCATGGGG CTGACCTTGG CTGAGAGTGA GCTGACTAAA
    CTTGATGCAG AGAGAGACAA GGACCGGGGA ACTCTGGAGA AGGAGCGAGC ATGTGCGGAA
    CAGATCGTTA CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAGG
    AGTTCCACAA TGCAGTATGT AATTCTCATG TATATGAAGT ATTTGGGAGT AAAAGTGAAA
    GAACCTCGAA ACTTGGAGCA CAAGCGGGGT CGGATCGGCT TCCTTCCAAA GATCAAGCAA
    AGCATGAAGA AAGATAGAGA GGGCGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATT
    TTGGGACCCC CAAGAAGACC AAGCCGCCAC GACAACAGTG CAATTGGCAG GGCCATGGAG
    ATACAGAAGT CACGCCACCC GAAGCACTTA TCCACTCCCT CGTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGGA GTAGCCAATG AAGGAACAGA CACTGGATAC
    CTGCCCGCCA GTTCCATGTC CTCTGCCACC TCAGGGACTG CTCTTTCCCA AGAGGGAGGG
    AGAGAGAATG ATACAGGAAC AAAACAAGTG GGAGAAGCAT CGGCGCCTGG AGACTGCTTG
    GACAGCACAC CTCGAGTCCC CACTACTGTT TTCGACTTCC CTCCTCCTCT ATTAGATCAA
    GTGCAGGAGG AGGAGTGTGA AGTAGAGAGG GTGGCAGAAC ATGGGACCCC AAAACCCTTT
    CGGAAGTTTG ACAGCATCGC CTTTGGAGAA AGTCAGAGTG AGGATGAGCA GTTTGAAAAT
    GACCTGGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GCAGAGAAGT GTTACTGGGA
    CTGAAACCTT CTGAGATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAGAGA
    GCTCATGTCC GGACACTGAA GGTTCTTGAC CAAGTATTCT ATCAGCGAGT GTCCAGAGAA
    GGGATTCTAT CACCTTCAGA ACTACGCAAG ATTTTTTCAA ACTTGGAAGA TATCCTTCAA
    CTTCATGTTG GGTTGAATGA GCAAATGAAA GCTGTCCGAA AAAGGAATGA AACTTCTGTT
    ATTGATCATA TTGGGGAAGA TCTGCTCATC TGGTTTAGTG GACCAGGAGA GGAGAAGCTG
    AAACATGCTG CTGCCACATT CTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT
    CGCCAGAAAA AGGATTCTCG ATTCCATACT TTTGTGCAAG ACGCTGAGAG TAACCCCCTG
    TGCCGTCGTC TTCAGCTGAA GGACATCATT CCCACCCAAA TGCAGAGACT CACTAAGTAC
    CCGCTTCTTT TGGATAACAT TGCCAAGTAC ACAGAATGGC CTCCAGAAAG GGAAAAGGTG
    AAGAAGGCTG CAGACCATTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAGGGAG
    GCAGAAAACA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TTGACACCTC CAATCTCAAG
    TTGTCCGAGT ACCCAAATGT TGACGAGCTC AGGAATTTGG ATTTAACAAA AAGAAAGATG
    ATTCATGAAG GGCCGTTGGT CTGGAAGGTG AATAGAGATA AAAGCATCGA TCTTTATACA
    CTGCTGCTGG AAGACATCCT TGTGCTGCTA CAGAAGCAGG ATGACAGGCT GGTGCTAAGG
    TGCCACAGCA AGATTCTGGC ATCCACAGCC GACAGCAAAC ACACCTTCAG CCCTGTCATT
    AAGCTGAGCA CGGTGCTGGT TCGGCAGGTG GCGACAGATA ACAAAGCTTT ATTCGTCATC
    TCCATGTCAG ATAATGGAGC CCAAATTTAT GAATTGGTAG CACAGACGGT TTCTGAAAAG
    ACAGTCTGGC AAGACCTCAT CTGTCGAATG GCTGCGTCAG TGAAGGAGCA GTCTACAAAG
    CCGATTCCCC TCCCACAGCC GCCACCTTGC GAAGGAGACA ATGATGAAGA AGAACCAGCC
    AAGTTAAAAG TGGAGCACCA TGATCTTTCA GTCGCTGGTC TGCAAAGTCC AGACAGAGTT
    TTGGGATTAG AATCTCCCTT AATTTCGTCA AAGCCTCAGT CTCATTCACT GAATACCCCT
    GGCAAATCAG CGGCAGAACA TCTCTTTGTG ACCGCAACAC AGTTTGCAAA GGAACAGCAT
    GCAAATGGGG CACTCAAGGA AGGTGATGGC GGGTATCCAG TCACAATCCC AGGTCCACAC
    TTGCCTGTGT CAGAGGAGCG GTGGGCATTG GACGCACTGA GAAATTTGGG CTTGTTGAAG
    CAGTTGCTGG TACAGCAGTT AGGTCTCACC GAGAAGAGTA CTCAGGAAGA CTGGCAGTCC
    TTCTCACGAT ACGGGCCAGC TTCTGAAGAG GTACAGGCAG ACAGTGGCAT CCGGGACTTG
    GAAAATGTTA AGGCTTGTCA TGCCAGGGAA GGACAGATGT CTTTTAAAAC TGGAACTGGT
    GATATTGCAA CTTGTGACAG TCCACGGACT TCAACTGAGT CTTGTGCTGC ACAGGATTCA
    GTGATACTGG CATCCCAAGA CAGCCAGGCA AGTAACGTTT TAGTAATGGA CCACATGATC
    CTGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGGGTC TGGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTGTTTGAT CCTTGATGGC
    TATGATGCAG TTCAGGAGAG CTCCACAGAT GAGGAGGTTG CCTCCTCGTT CCCCCTGCAG
    CCTGTGACGG GCATCCCCGC TGTGGACTCC AGCCACCAGC AGCAACATTC CCCTCAGAAT
    GTGCATCCCG AGGGGCCGGT TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCGGCACTGG
    CGAGCTATGG AGGATACCTG TTTTGAGATC CAGAGTCCCT CATGTACAGA TTCACAAAGC
    CAAATCCTGG AGTACATTCA TAAAATAGAG GCTGACCTGG AACACTTAAA GAAGGTGGAG
    GAAAGTTACG CTCTTCTTTG CCAAAGGCTG GCTGGCTCAG CCCTCCCCGA CAAGCTCTCA
    GATAAAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006510649.1
    Translation

    Target ORF information:

    RefSeq Version XM_006510586.2
    Organism Mus musculus(House mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006510586.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTG AGCCGCAGTC GCTGGTCGGC
    AGTCCCTCCA CCCGGGGCGC CCCTCACATC ATTGGAGCAG AGGATGATGA TTTTGGTACT
    GAGCATGAGC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT GCTGAAGTCT
    CGCCCTGCTC ACTTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCTGCAACA
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAGCAGACAA ACTCCAAGGA GACTCGGCGT
    GTCTTCCTGG AGTTTCATCA GTTCTTCTTG GACCGGTCTG CACACCTGAA GGTTCCAGTT
    CCTGAGGAAA TCTCCGTGGA TCTAGAAAAG AGAAGACCTG AGCTTATTCC TGAGGATCTG
    CATCGCCTCT ACATTCAGAC AATGCAGGAG AGAGTCCACC CTGAAGTTCA GAGGCACTTG
    GAGGATTTCC GACAGAAACG CAGCATGGGG CTGACCTTGG CTGAGAGTGA GCTGACTAAA
    CTTGATGCAG AGAGAGACAA GGACCGGGGA ACTCTGGAGA AGGAGCGAGC ATGTGCGGAA
    CAGATCGTTA CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAGG
    AGTTCCACAA TGCAGTATGT AATTCTCATG TATATGAAGT ATTTGGGAGT AAAAGTGAAA
    GAACCTCGAA ACTTGGAGCA CAAGCGGGGT CGGATCGGCT TCCTTCCAAA GATCAAGCAA
    AGCATGAAGA AAGATAGAGA GGGCGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATT
    TTGGGACCCC CAAGAAGACC AAGCCGCCAC GACAACAGTG CAATTGGCAG GGCCATGGAG
    ATACAGAAGT CACGCCACCC GAAGCACTTA TCCACTCCCT CGTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGGA GTAGCCAATG AAGGAACAGA CACTGGATAC
    CTGCCCGCCA GTTCCATGTC CTCTGCCACC TCAGGGACTG CTCTTTCCCA AGAGGGAGGG
    AGAGAGAATG ATACAGGAAC AAAACAAGTG GGAGAAGCAT CGGCGCCTGG AGACTGCTTG
    GACAGCACAC CTCGAGTCCC CACTACTGTT TTCGACTTCC CTCCTCCTCT ATTAGATCAA
    GTGCAGGAGG AGGAGTGTGA AGTAGAGAGG GTGGCAGAAC ATGGGACCCC AAAACCCTTT
    CGGAAGTTTG ACAGCATCGC CTTTGGAGAA AGTCAGAGTG AGGATGAGCA GTTTGAAAAT
    GACCTGGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GCAGAGAAGT GTTACTGGGA
    CTGAAACCTT CTGAGATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAGAGA
    GCTCATGTCC GGACACTGAA GGTTCTTGAC CAAGTATTCT ATCAGCGAGT GTCCAGAGAA
    GGGATTCTAT CACCTTCAGA ACTACGCAAG ATTTTTTCAA ACTTGGAAGA TATCCTTCAA
    CTTCATGTTG GGTTGAATGA GCAAATGAAA GCTGTCCGAA AAAGGAATGA AACTTCTGTT
    ATTGATCATA TTGGGGAAGA TCTGCTCATC TGGTTTAGTG GACCAGGAGA GGAGAAGCTG
    AAACATGCTG CTGCCACATT CTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT
    CGCCAGAAAA AGGATTCTCG ATTCCATACT TTTGTGCAAG ACGCTGAGAG TAACCCCCTG
    TGCCGTCGTC TTCAGCTGAA GGACATCATT CCCACCCAAA TGCAGAGACT CACTAAGTAC
    CCGCTTCTTT TGGATAACAT TGCCAAGTAC ACAGAATGGC CTCCAGAAAG GGAAAAGGTG
    AAGAAGGCTG CAGACCATTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAGGGAG
    GCAGAAAACA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TTGACACCTC CAATCTCAAG
    TTGTCCGAGT ACCCAAATGT TGACGAGCTC AGGAATTTGG ATTTAACAAA AAGAAAGATG
    ATTCATGAAG GGCCGTTGGT CTGGAAGGTG AATAGAGATA AAAGCATCGA TCTTTATACA
    CTGCTGCTGG AAGACATCCT TGTGCTGCTA CAGAAGCAGG ATGACAGGCT GGTGCTAAGG
    TGCCACAGCA AGATTCTGGC ATCCACAGCC GACAGCAAAC ACACCTTCAG CCCTGTCATT
    AAGCTGAGCA CGGTGCTGGT TCGGCAGGTG GCGACAGATA ACAAAGCTTT ATTCGTCATC
    TCCATGTCAG ATAATGGAGC CCAAATTTAT GAATTGGTAG CACAGACGGT TTCTGAAAAG
    ACAGTCTGGC AAGACCTCAT CTGTCGAATG GCTGCGTCAG TGAAGGAGCA GTCTACAAAG
    CCGATTCCCC TCCCACAGCC GCCACCTTGC GAAGGAGACA ATGATGAAGA AGAACCAGCC
    AAGTTAAAAG TGGAGCACCA TGATCTTTCA GTCGCTGGTC TGCAAAGTCC AGACAGAGTT
    TTGGGATTAG AATCTCCCTT AATTTCGTCA AAGCCTCAGT CTCATTCACT GAATACCCCT
    GGCAAATCAG CGGCAGAACA TCTCTTTGTG ACCGCAACAC AGTTTGCAAA GGAACAGCAT
    GCAAATGGGG CACTCAAGGA AGGTGATGGC GGGTATCCAG TCACAATCCC AGGTCCACAC
    TTGCCTGTGT CAGAGGAGCG GTGGGCATTG GACGCACTGA GAAATTTGGG CTTGTTGAAG
    CAGTTGCTGG TACAGCAGTT AGGTCTCACC GAGAAGAGTA CTCAGGAAGA CTGGCAGTCC
    TTCTCACGAT ACGGGCCAGC TTCTGAAGAG GTACAGGCAG ACAGTGGCAT CCGGGACTTG
    GAAAATGTTA AGGCTTGTCA TGCCAGGGAA GGACAGATGT CTTTTAAAAC TGGAACTGGT
    GATATTGCAA CTTGTGACAG TCCACGGACT TCAACTGAGT CTTGTGCTGC ACAGGATTCA
    GTGATACTGG CATCCCAAGA CAGCCAGGCA AGTAACGTTT TAGTAATGGA CCACATGATC
    CTGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGGGTC TGGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTGTTTGAT CCTTGATGGC
    TATGATGCAG TTCAGGAGAG CTCCACAGAT GAGGAGGTTG CCTCCTCGTT CCCCCTGCAG
    CCTGTGACGG GCATCCCCGC TGTGGACTCC AGCCACCAGC AGCAACATTC CCCTCAGAAT
    GTGCATCCCG AGGGGCCGGT TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCGGCACTGG
    CGAGCTATGG AGGATACCTG TTTTGAGATC CAGAGTCCCT CATGTACAGA TTCACAAAGC
    CAAATCCTGG AGTACATTCA TAAAATAGAG GCTGACCTGG AACACTTAAA GAAGGTGGAG
    GAAAGTTACG CTCTTCTTTG CCAAAGGCTG GCTGGCTCAG CCCTCCCCGA CAAGCTCTCA
    GATAAAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu42386
    Clone ID Related Accession (Same CDS sequence) XM_006510586.2 , NM_001359232.1 , XM_006510586.3
    Accession Version XM_006510586.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(House mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006510586.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTG AGCCGCAGTC GCTGGTCGGC
    AGTCCCTCCA CCCGGGGCGC CCCTCACATC ATTGGAGCAG AGGATGATGA TTTTGGTACT
    GAGCATGAGC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT GCTGAAGTCT
    CGCCCTGCTC ACTTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCTGCAACA
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAGCAGACAA ACTCCAAGGA GACTCGGCGT
    GTCTTCCTGG AGTTTCATCA GTTCTTCTTG GACCGGTCTG CACACCTGAA GGTTCCAGTT
    CCTGAGGAAA TCTCCGTGGA TCTAGAAAAG AGAAGACCTG AGCTTATTCC TGAGGATCTG
    CATCGCCTCT ACATTCAGAC AATGCAGGAG AGAGTCCACC CTGAAGTTCA GAGGCACTTG
    GAGGATTTCC GACAGAAACG CAGCATGGGG CTGACCTTGG CTGAGAGTGA GCTGACTAAA
    CTTGATGCAG AGAGAGACAA GGACCGGGGA ACTCTGGAGA AGGAGCGAGC ATGTGCGGAA
    CAGATCGTTA CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAGG
    AGTTCCACAA TGCAGTATGT AATTCTCATG TATATGAAGT ATTTGGGAGT AAAAGTGAAA
    GAACCTCGAA ACTTGGAGCA CAAGCGGGGT CGGATCGGCT TCCTTCCAAA GATCAAGCAA
    AGCATGAAGA AAGATAGAGA GGGCGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATT
    TTGGGACCCC CAAGAAGACC AAGCCGCCAC GACAACAGTG CAATTGGCAG GGCCATGGAG
    ATACAGAAGT CACGCCACCC GAAGCACTTA TCCACTCCCT CGTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGGA GTAGCCAATG AAGGAACAGA CACTGGATAC
    CTGCCCGCCA GTTCCATGTC CTCTGCCACC TCAGGGACTG CTCTTTCCCA AGAGGGAGGG
    AGAGAGAATG ATACAGGAAC AAAACAAGTG GGAGAAGCAT CGGCGCCTGG AGACTGCTTG
    GACAGCACAC CTCGAGTCCC CACTACTGTT TTCGACTTCC CTCCTCCTCT ATTAGATCAA
    GTGCAGGAGG AGGAGTGTGA AGTAGAGAGG GTGGCAGAAC ATGGGACCCC AAAACCCTTT
    CGGAAGTTTG ACAGCATCGC CTTTGGAGAA AGTCAGAGTG AGGATGAGCA GTTTGAAAAT
    GACCTGGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GCAGAGAAGT GTTACTGGGA
    CTGAAACCTT CTGAGATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAGAGA
    GCTCATGTCC GGACACTGAA GGTTCTTGAC CAAGTATTCT ATCAGCGAGT GTCCAGAGAA
    GGGATTCTAT CACCTTCAGA ACTACGCAAG ATTTTTTCAA ACTTGGAAGA TATCCTTCAA
    CTTCATGTTG GGTTGAATGA GCAAATGAAA GCTGTCCGAA AAAGGAATGA AACTTCTGTT
    ATTGATCATA TTGGGGAAGA TCTGCTCATC TGGTTTAGTG GACCAGGAGA GGAGAAGCTG
    AAACATGCTG CTGCCACATT CTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT
    CGCCAGAAAA AGGATTCTCG ATTCCATACT TTTGTGCAAG ACGCTGAGAG TAACCCCCTG
    TGCCGTCGTC TTCAGCTGAA GGACATCATT CCCACCCAAA TGCAGAGACT CACTAAGTAC
    CCGCTTCTTT TGGATAACAT TGCCAAGTAC ACAGAATGGC CTCCAGAAAG GGAAAAGGTG
    AAGAAGGCTG CAGACCATTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAGGGAG
    GCAGAAAACA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TTGACACCTC CAATCTCAAG
    TTGTCCGAGT ACCCAAATGT TGACGAGCTC AGGAATTTGG ATTTAACAAA AAGAAAGATG
    ATTCATGAAG GGCCGTTGGT CTGGAAGGTG AATAGAGATA AAAGCATCGA TCTTTATACA
    CTGCTGCTGG AAGACATCCT TGTGCTGCTA CAGAAGCAGG ATGACAGGCT GGTGCTAAGG
    TGCCACAGCA AGATTCTGGC ATCCACAGCC GACAGCAAAC ACACCTTCAG CCCTGTCATT
    AAGCTGAGCA CGGTGCTGGT TCGGCAGGTG GCGACAGATA ACAAAGCTTT ATTCGTCATC
    TCCATGTCAG ATAATGGAGC CCAAATTTAT GAATTGGTAG CACAGACGGT TTCTGAAAAG
    ACAGTCTGGC AAGACCTCAT CTGTCGAATG GCTGCGTCAG TGAAGGAGCA GTCTACAAAG
    CCGATTCCCC TCCCACAGCC GCCACCTTGC GAAGGAGACA ATGATGAAGA AGAACCAGCC
    AAGTTAAAAG TGGAGCACCA TGATCTTTCA GTCGCTGGTC TGCAAAGTCC AGACAGAGTT
    TTGGGATTAG AATCTCCCTT AATTTCGTCA AAGCCTCAGT CTCATTCACT GAATACCCCT
    GGCAAATCAG CGGCAGAACA TCTCTTTGTG ACCGCAACAC AGTTTGCAAA GGAACAGCAT
    GCAAATGGGG CACTCAAGGA AGGTGATGGC GGGTATCCAG TCACAATCCC AGGTCCACAC
    TTGCCTGTGT CAGAGGAGCG GTGGGCATTG GACGCACTGA GAAATTTGGG CTTGTTGAAG
    CAGTTGCTGG TACAGCAGTT AGGTCTCACC GAGAAGAGTA CTCAGGAAGA CTGGCAGTCC
    TTCTCACGAT ACGGGCCAGC TTCTGAAGAG GTACAGGCAG ACAGTGGCAT CCGGGACTTG
    GAAAATGTTA AGGCTTGTCA TGCCAGGGAA GGACAGATGT CTTTTAAAAC TGGAACTGGT
    GATATTGCAA CTTGTGACAG TCCACGGACT TCAACTGAGT CTTGTGCTGC ACAGGATTCA
    GTGATACTGG CATCCCAAGA CAGCCAGGCA AGTAACGTTT TAGTAATGGA CCACATGATC
    CTGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGGGTC TGGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTGTTTGAT CCTTGATGGC
    TATGATGCAG TTCAGGAGAG CTCCACAGAT GAGGAGGTTG CCTCCTCGTT CCCCCTGCAG
    CCTGTGACGG GCATCCCCGC TGTGGACTCC AGCCACCAGC AGCAACATTC CCCTCAGAAT
    GTGCATCCCG AGGGGCCGGT TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCGGCACTGG
    CGAGCTATGG AGGATACCTG TTTTGAGATC CAGAGTCCCT CATGTACAGA TTCACAAAGC
    CAAATCCTGG AGTACATTCA TAAAATAGAG GCTGACCTGG AACACTTAAA GAAGGTGGAG
    GAAAGTTACG CTCTTCTTTG CCAAAGGCTG GCTGGCTCAG CCCTCCCCGA CAAGCTCTCA
    GATAAAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006510649.1
    CDS277..4908
    Translation

    Target ORF information:

    RefSeq Version XM_006510586.3
    Organism Mus musculus(House mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006510586.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATCTATGG TCTTGTTCAG CGCTGTGTGA TCATCCAGAA AGACGACAAT
    GGCTTCGGGC TGACAGTCAG TGGAGACAAT CCGGTCTTCG TACAATCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGTGCTGG AGTACAAACA GGTGATCGAA TCATCAAGGT GAATGGTACT
    CTGGTGACTC ATTCAAATCA TCTGGAGGTG GTGAAACTAA TCAGATCTGG TTCCTATGTA
    GCTCTCACTG TCCAGGGACG CCCACCTGGG TCGCCCCAGA TCCCACTTGC TGATTCTGAA
    GTAGAGCCAT CAGTCACTGG ACATATGTCT CCCATTATGA CATCCCCTCA TTCCCCTGGA
    GCAGCCGGGA ATATGGAGAG AATCACCAGT CCTGTGCTCG TGGGGGAGGA AAACAATGTG
    GTCCATAACC AGAAAGTAGA GATTCTGAGA AAGATGTTAC AGAAGGAACA GGAACGGCTG
    CAGTTGCTGC AGGAAGATTA CAACCGAACG GCTACCCAAA GATTGCTCAA GGAGATCCAG
    GAGGCCAAGA AGCACATCCC GCAGCTGCAG GAGCAGCTGT CCAAAGCCAC GGGCTCTGCT
    CAGGACGGAG CTGTAATTGC ACCCTCCAGG CCATTAGGTG ATGCCCTAAC ACTCAGTGAG
    GCAGAAGCAG ATCCTGGAGA TGGCCTGTGC AGGACTGACT GGAGCAGTGG AGATGCTTCT
    CGGCCCAGTA GTGACAGTGC TGATAGTCCC AAGAGTAGCC TGAGAGAAAG GAGTTACCTG
    GAGGAAGCCC CAGAGAGAAG TGAAGGAGTT CAGGACGCTG AGCCGCAGTC GCTGGTCGGC
    AGTCCCTCCA CCCGGGGCGC CCCTCACATC ATTGGAGCAG AGGATGATGA TTTTGGTACT
    GAGCATGAGC AGATCAATGG ACAGTGCAGC TGTTTCCAGA GCATTGAATT GCTGAAGTCT
    CGCCCTGCTC ACTTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCTGCAACA
    TTGCTCTGTT ATCTCTATTC AGACCTGTAT AAGCAGACAA ACTCCAAGGA GACTCGGCGT
    GTCTTCCTGG AGTTTCATCA GTTCTTCTTG GACCGGTCTG CACACCTGAA GGTTCCAGTT
    CCTGAGGAAA TCTCCGTGGA TCTAGAAAAG AGAAGACCTG AGCTTATTCC TGAGGATCTG
    CATCGCCTCT ACATTCAGAC AATGCAGGAG AGAGTCCACC CTGAAGTTCA GAGGCACTTG
    GAGGATTTCC GACAGAAACG CAGCATGGGG CTGACCTTGG CTGAGAGTGA GCTGACTAAA
    CTTGATGCAG AGAGAGACAA GGACCGGGGA ACTCTGGAGA AGGAGCGAGC ATGTGCGGAA
    CAGATCGTTA CCAAAATTGA AGAAGTGTTG ATGACTGCTC AGGCTGTAGA AGAAGAAAGG
    AGTTCCACAA TGCAGTATGT AATTCTCATG TATATGAAGT ATTTGGGAGT AAAAGTGAAA
    GAACCTCGAA ACTTGGAGCA CAAGCGGGGT CGGATCGGCT TCCTTCCAAA GATCAAGCAA
    AGCATGAAGA AAGATAGAGA GGGCGAAGAA AAAGGGAAGC GAAGAGGATT CCCCAGCATT
    TTGGGACCCC CAAGAAGACC AAGCCGCCAC GACAACAGTG CAATTGGCAG GGCCATGGAG
    ATACAGAAGT CACGCCACCC GAAGCACTTA TCCACTCCCT CGTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGGA GTAGCCAATG AAGGAACAGA CACTGGATAC
    CTGCCCGCCA GTTCCATGTC CTCTGCCACC TCAGGGACTG CTCTTTCCCA AGAGGGAGGG
    AGAGAGAATG ATACAGGAAC AAAACAAGTG GGAGAAGCAT CGGCGCCTGG AGACTGCTTG
    GACAGCACAC CTCGAGTCCC CACTACTGTT TTCGACTTCC CTCCTCCTCT ATTAGATCAA
    GTGCAGGAGG AGGAGTGTGA AGTAGAGAGG GTGGCAGAAC ATGGGACCCC AAAACCCTTT
    CGGAAGTTTG ACAGCATCGC CTTTGGAGAA AGTCAGAGTG AGGATGAGCA GTTTGAAAAT
    GACCTGGAGA CAGATCCACC CAACTGGCAG CAGCTTGTTA GCAGAGAAGT GTTACTGGGA
    CTGAAACCTT CTGAGATCAA AAGACAGGAA GTGATTAATG AATTGTTCTA CACTGAGAGA
    GCTCATGTCC GGACACTGAA GGTTCTTGAC CAAGTATTCT ATCAGCGAGT GTCCAGAGAA
    GGGATTCTAT CACCTTCAGA ACTACGCAAG ATTTTTTCAA ACTTGGAAGA TATCCTTCAA
    CTTCATGTTG GGTTGAATGA GCAAATGAAA GCTGTCCGAA AAAGGAATGA AACTTCTGTT
    ATTGATCATA TTGGGGAAGA TCTGCTCATC TGGTTTAGTG GACCAGGAGA GGAGAAGCTG
    AAACATGCTG CTGCCACATT CTGCAGTAAC CAACCTTTCG CCCTGGAAAT GATCAAGTCT
    CGCCAGAAAA AGGATTCTCG ATTCCATACT TTTGTGCAAG ACGCTGAGAG TAACCCCCTG
    TGCCGTCGTC TTCAGCTGAA GGACATCATT CCCACCCAAA TGCAGAGACT CACTAAGTAC
    CCGCTTCTTT TGGATAACAT TGCCAAGTAC ACAGAATGGC CTCCAGAAAG GGAAAAGGTG
    AAGAAGGCTG CAGACCATTG TCGTCAGATC TTAAATTATG TAAATCAGGC TGTCAGGGAG
    GCAGAAAACA AGCAGCGCTT AGAAGACTAT CAGCGTCGCC TTGACACCTC CAATCTCAAG
    TTGTCCGAGT ACCCAAATGT TGACGAGCTC AGGAATTTGG ATTTAACAAA AAGAAAGATG
    ATTCATGAAG GGCCGTTGGT CTGGAAGGTG AATAGAGATA AAAGCATCGA TCTTTATACA
    CTGCTGCTGG AAGACATCCT TGTGCTGCTA CAGAAGCAGG ATGACAGGCT GGTGCTAAGG
    TGCCACAGCA AGATTCTGGC ATCCACAGCC GACAGCAAAC ACACCTTCAG CCCTGTCATT
    AAGCTGAGCA CGGTGCTGGT TCGGCAGGTG GCGACAGATA ACAAAGCTTT ATTCGTCATC
    TCCATGTCAG ATAATGGAGC CCAAATTTAT GAATTGGTAG CACAGACGGT TTCTGAAAAG
    ACAGTCTGGC AAGACCTCAT CTGTCGAATG GCTGCGTCAG TGAAGGAGCA GTCTACAAAG
    CCGATTCCCC TCCCACAGCC GCCACCTTGC GAAGGAGACA ATGATGAAGA AGAACCAGCC
    AAGTTAAAAG TGGAGCACCA TGATCTTTCA GTCGCTGGTC TGCAAAGTCC AGACAGAGTT
    TTGGGATTAG AATCTCCCTT AATTTCGTCA AAGCCTCAGT CTCATTCACT GAATACCCCT
    GGCAAATCAG CGGCAGAACA TCTCTTTGTG ACCGCAACAC AGTTTGCAAA GGAACAGCAT
    GCAAATGGGG CACTCAAGGA AGGTGATGGC GGGTATCCAG TCACAATCCC AGGTCCACAC
    TTGCCTGTGT CAGAGGAGCG GTGGGCATTG GACGCACTGA GAAATTTGGG CTTGTTGAAG
    CAGTTGCTGG TACAGCAGTT AGGTCTCACC GAGAAGAGTA CTCAGGAAGA CTGGCAGTCC
    TTCTCACGAT ACGGGCCAGC TTCTGAAGAG GTACAGGCAG ACAGTGGCAT CCGGGACTTG
    GAAAATGTTA AGGCTTGTCA TGCCAGGGAA GGACAGATGT CTTTTAAAAC TGGAACTGGT
    GATATTGCAA CTTGTGACAG TCCACGGACT TCAACTGAGT CTTGTGCTGC ACAGGATTCA
    GTGATACTGG CATCCCAAGA CAGCCAGGCA AGTAACGTTT TAGTAATGGA CCACATGATC
    CTGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGGGTC TGGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAAAAGGAAG AGAAGGATGT TAATTTACGC ATCTCAGGAA ACTGTTTGAT CCTTGATGGC
    TATGATGCAG TTCAGGAGAG CTCCACAGAT GAGGAGGTTG CCTCCTCGTT CCCCCTGCAG
    CCTGTGACGG GCATCCCCGC TGTGGACTCC AGCCACCAGC AGCAACATTC CCCTCAGAAT
    GTGCATCCCG AGGGGCCGGT TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCGGCACTGG
    CGAGCTATGG AGGATACCTG TTTTGAGATC CAGAGTCCCT CATGTACAGA TTCACAAAGC
    CAAATCCTGG AGTACATTCA TAAAATAGAG GCTGACCTGG AACACTTAAA GAAGGTGGAG
    GAAAGTTACG CTCTTCTTTG CCAAAGGCTG GCTGGCTCAG CCCTCCCCGA CAAGCTCTCA
    GATAAAAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu70443
    Clone ID Related Accession (Same CDS sequence) XM_011242597.1 , XM_011242597.2
    Accession Version XM_011242597.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTGAGCCGCA GTCGCTGGTC
    GGCAGTCCCT CCACCCGGGG CGCCCCTCAC ATCATTGGAG CAGAGGATGA TGATTTTGGT
    ACTGAGCATG AGCAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTGCTGAAG
    TCTCGCCCTG CTCACTTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCTGCA
    ACATTGCTCT GTTATCTCTA TTCAGACCTG TATAAGCAGA CAAACTCCAA GGAGACTCGG
    CGTGTCTTCC TGGAGTTTCA TCAGTTCTTC TTGGACCGGT CTGCACACCT GAAGGTTCCA
    GTTCCTGAGG AAATCTCCGT GGATCTAGAA AAGAGAAGAC CTGAGCTTAT TCCTGAGGAT
    CTGCATCGCC TCTACATTCA GACAATGCAG GAGAGAGTCC ACCCTGAAGT TCAGAGGCAC
    TTGGAGGATT TCCGACAGAA ACGCAGCATG GGGCTGACCT TGGCTGAGAG TGAGCTGACT
    AAACTTGATG CAGAGAGAGA CAAGGACCGG GGAACTCTGG AGAAGGAGCG AGCATGTGCG
    GAACAGATCG TTACCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA
    AGGAGTTCCA CAATGCAGTA TGTAATTCTC ATGTATATGA AGTATTTGGG AGTAAAAGTG
    AAAGAACCTC GAAACTTGGA GCACAAGCGG GGTCGGATCG GCTTCCTTCC AAAGATCAAG
    CAAAGCATGA AGAAAGATAG AGAGGGCGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATTTTGGGAC CCCCAAGAAG ACCAAGCCGC CACGACAACA GTGCAATTGG CAGGGCCATG
    GAGATACAGA AGTCACGCCA CCCGAAGCAC TTATCCACTC CCTCGTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GGAGTAGCCA ATGAAGGAAC AGACACTGGA
    TACCTGCCCG CCAGTTCCAT GTCCTCTGCC ACCTCAGGGA CTGCTCTTTC CCAAGAGGGA
    GGGAGAGAGA ATGATACAGG AACAAAACAA GTGGGAGAAG CATCGGCGCC TGGAGACTGC
    TTGGACAGCA CACCTCGAGT CCCCACTACT GTTTTCGACT TCCCTCCTCC TCTATTAGAT
    CAAGTGCAGG AGGAGGAGTG TGAAGTAGAG AGGGTGGCAG AACATGGGAC CCCAAAACCC
    TTTCGGAAGT TTGACAGCAT CGCCTTTGGA GAAAGTCAGA GTGAGGATGA GCAGTTTGAA
    AATGACCTGG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGCAGAGA AGTGTTACTG
    GGACTGAAAC CTTCTGAGAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAG
    AGAGCTCATG TCCGGACACT GAAGGTTCTT GACCAAGTAT TCTATCAGCG AGTGTCCAGA
    GAAGGGATTC TATCACCTTC AGAACTACGC AAGATTTTTT CAAACTTGGA AGATATCCTT
    CAACTTCATG TTGGGTTGAA TGAGCAAATG AAAGCTGTCC GAAAAAGGAA TGAAACTTCT
    GTTATTGATC ATATTGGGGA AGATCTGCTC ATCTGGTTTA GTGGACCAGG AGAGGAGAAG
    CTGAAACATG CTGCTGCCAC ATTCTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG
    TCTCGCCAGA AAAAGGATTC TCGATTCCAT ACTTTTGTGC AAGACGCTGA GAGTAACCCC
    CTGTGCCGTC GTCTTCAGCT GAAGGACATC ATTCCCACCC AAATGCAGAG ACTCACTAAG
    TACCCGCTTC TTTTGGATAA CATTGCCAAG TACACAGAAT GGCCTCCAGA AAGGGAAAAG
    GTGAAGAAGG CTGCAGACCA TTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAGG
    GAGGCAGAAA ACAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTTGACAC CTCCAATCTC
    AAGTTGTCCG AGTACCCAAA TGTTGACGAG CTCAGGAATT TGGATTTAAC AAAAAGAAAG
    ATGATTCATG AAGGGCCGTT GGTCTGGAAG GTGAATAGAG ATAAAAGCAT CGATCTTTAT
    ACACTGCTGC TGGAAGACAT CCTTGTGCTG CTACAGAAGC AGGATGACAG GCTGGTGCTA
    AGGTGCCACA GCAAGATTCT GGCATCCACA GCCGACAGCA AACACACCTT CAGCCCTGTC
    ATTAAGCTGA GCACGGTGCT GGTTCGGCAG GTGGCGACAG ATAACAAAGC TTTATTCGTC
    ATCTCCATGT CAGATAATGG AGCCCAAATT TATGAATTGG TAGCACAGAC GGTTTCTGAA
    AAGACAGTCT GGCAAGACCT CATCTGTCGA ATGGCTGCGT CAGTGAAGGA GCAGTCTACA
    AAGCCGATTC CCCTCCCACA GCCGCCACCT TGCGAAGGAG ACAATGATGA AGAAGAACCA
    GCCAAGTTAA AAGTGGAGCA CCATGATCTT TCAGTCGCTG GTCTGCAAAG TCCAGACAGA
    GTTTTGGGAT TAGAATCTCC CTTAATTTCG TCAAAGCCTC AGTCTCATTC ACTGAATACC
    CCTGGCAAAT CAGCGGCAGA ACATCTCTTT GTGACCGCAA CACAGTTTGC AAAGGAACAG
    CATGCAAATG GGGCACTCAA GGAAGGTGAT GGCGGGTATC CAGTCACAAT CCCAGGTCCA
    CACTTGCCTG TGTCAGAGGA GCGGTGGGCA TTGGACGCAC TGAGAAATTT GGGCTTGTTG
    AAGCAGTTGC TGGTACAGCA GTTAGGTCTC ACCGAGAAGA GTACTCAGGA AGACTGGCAG
    TCCTTCTCAC GATACGGGCC AGCTTCTGAA GAGGTACAGG CAGACAGTGG CATCCGGGAC
    TTGGAAAATG TTAAGGCTTG TCATGCCAGG GAAGGACAGA TGTCTTTTAA AACTGGAACT
    GGTGATATTG CAACTTGTGA CAGTCCACGG ACTTCAACTG AGTCTTGTGC TGCACAGGAT
    TCAGTGATAC TGGCATCCCA AGACAGCCAG GCAAGTAACG TTTTAGTAAT GGACCACATG
    ATCCTGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGG GTCTGGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTGTTT GATCCTTGAT
    GGCTATGATG CAGTTCAGGA GAGCTCCACA GATGAGGAGG TTGCCTCCTC GTTCCCCCTG
    CAGCCTGTGA CGGGCATCCC CGCTGTGGAC TCCAGCCACC AGCAGCAACA TTCCCCTCAG
    AATGTGCATC CCGAGGGGCC GGTTTCACCA TTCACCCCAG AATTCCTGGT CCAGCGGCAC
    TGGCGAGCTA TGGAGGATAC CTGTTTTGAG ATCCAGAGTC CCTCATGTAC AGATTCACAA
    AGCCAAATCC TGGAGTACAT TCATAAAATA GAGGCTGACC TGGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACGCTCTTCT TTGCCAAAGG CTGGCTGGCT CAGCCCTCCC CGACAAGCTC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011240899.1
    CDS45..4619
    Translation

    Target ORF information:

    RefSeq Version XM_011242597.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011242597.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGTGGCA CACAGTCTAC TATCACGGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAAC AGAAAGTTGA ACGCAGTTCA
    TCGCATGATT TTGACCCCAC AGGTCTTGTT CAGCGCTGTG TGATCATCCA GAAAGACGAC
    AATGGCTTCG GGCTGACAGT CAGTGGAGAC AATCCGGTCT TCGTACAATC TGTCAAAGAA
    GATGGAGCAG CCATGCGTGC TGGAGTACAA ACAGGTGATC GAATCATCAA GGTGAATGGT
    ACTCTGGTGA CTCATTCAAA TCATCTGGAG GTGGTGAAAC TAATCAGATC TGGTTCCTAT
    GTAGCTCTCA CTGTCCAGGG ACGCCCACCT GGGTCGCCCC AGATCCCACT TGCTGATTCT
    GAAGTAGAGC CATCAGTCAC TGGACATATG TCTCCCATTA TGACATCCCC TCATTCCCCT
    GGAGCAGCCG GGAATATGGA GAGAATCACC AGTCCTGTGC TCGTGGGGGA GGAAAACAAT
    GTGGTCCATA ACCAGAAAGT AGAGATTCTG AGAAAGATGT TACAGAAGGA ACAGGAACGG
    CTGCAGTTGC TGCAGGAAGA TTACAACCGA ACGGCTACCC AAAGATTGCT CAAGGAGATC
    CAGGAGGCCA AGAAGCACAT CCCGCAGCTG CAGGAGCAGC TGTCCAAAGC CACGGGCTCT
    GCTCAGGACG GAGCTGTAAT TGCACCCTCC AGGCCATTAG GTGATGCCCT AACACTCAGT
    GAGGCAGAAG CAGATCCTGG AGATGGCCTG TGCAGGACTG ACTGGAGCAG TGGAGATGCT
    TCTCGGCCCA GTAGTGACAG TGCTGATAGT CCCAAGAGTA GCCTGAGAGA AAGGAGTTAC
    CTGGAGGAAG CCCCAGAGAG AAGTGAAGGA GTTCAGGACG CTGAGCCGCA GTCGCTGGTC
    GGCAGTCCCT CCACCCGGGG CGCCCCTCAC ATCATTGGAG CAGAGGATGA TGATTTTGGT
    ACTGAGCATG AGCAGATCAA TGGACAGTGC AGCTGTTTCC AGAGCATTGA ATTGCTGAAG
    TCTCGCCCTG CTCACTTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCTGCA
    ACATTGCTCT GTTATCTCTA TTCAGACCTG TATAAGCAGA CAAACTCCAA GGAGACTCGG
    CGTGTCTTCC TGGAGTTTCA TCAGTTCTTC TTGGACCGGT CTGCACACCT GAAGGTTCCA
    GTTCCTGAGG AAATCTCCGT GGATCTAGAA AAGAGAAGAC CTGAGCTTAT TCCTGAGGAT
    CTGCATCGCC TCTACATTCA GACAATGCAG GAGAGAGTCC ACCCTGAAGT TCAGAGGCAC
    TTGGAGGATT TCCGACAGAA ACGCAGCATG GGGCTGACCT TGGCTGAGAG TGAGCTGACT
    AAACTTGATG CAGAGAGAGA CAAGGACCGG GGAACTCTGG AGAAGGAGCG AGCATGTGCG
    GAACAGATCG TTACCAAAAT TGAAGAAGTG TTGATGACTG CTCAGGCTGT AGAAGAAGAA
    AGGAGTTCCA CAATGCAGTA TGTAATTCTC ATGTATATGA AGTATTTGGG AGTAAAAGTG
    AAAGAACCTC GAAACTTGGA GCACAAGCGG GGTCGGATCG GCTTCCTTCC AAAGATCAAG
    CAAAGCATGA AGAAAGATAG AGAGGGCGAA GAAAAAGGGA AGCGAAGAGG ATTCCCCAGC
    ATTTTGGGAC CCCCAAGAAG ACCAAGCCGC CACGACAACA GTGCAATTGG CAGGGCCATG
    GAGATACAGA AGTCACGCCA CCCGAAGCAC TTATCCACTC CCTCGTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GGAGTAGCCA ATGAAGGAAC AGACACTGGA
    TACCTGCCCG CCAGTTCCAT GTCCTCTGCC ACCTCAGGGA CTGCTCTTTC CCAAGAGGGA
    GGGAGAGAGA ATGATACAGG AACAAAACAA GTGGGAGAAG CATCGGCGCC TGGAGACTGC
    TTGGACAGCA CACCTCGAGT CCCCACTACT GTTTTCGACT TCCCTCCTCC TCTATTAGAT
    CAAGTGCAGG AGGAGGAGTG TGAAGTAGAG AGGGTGGCAG AACATGGGAC CCCAAAACCC
    TTTCGGAAGT TTGACAGCAT CGCCTTTGGA GAAAGTCAGA GTGAGGATGA GCAGTTTGAA
    AATGACCTGG AGACAGATCC ACCCAACTGG CAGCAGCTTG TTAGCAGAGA AGTGTTACTG
    GGACTGAAAC CTTCTGAGAT CAAAAGACAG GAAGTGATTA ATGAATTGTT CTACACTGAG
    AGAGCTCATG TCCGGACACT GAAGGTTCTT GACCAAGTAT TCTATCAGCG AGTGTCCAGA
    GAAGGGATTC TATCACCTTC AGAACTACGC AAGATTTTTT CAAACTTGGA AGATATCCTT
    CAACTTCATG TTGGGTTGAA TGAGCAAATG AAAGCTGTCC GAAAAAGGAA TGAAACTTCT
    GTTATTGATC ATATTGGGGA AGATCTGCTC ATCTGGTTTA GTGGACCAGG AGAGGAGAAG
    CTGAAACATG CTGCTGCCAC ATTCTGCAGT AACCAACCTT TCGCCCTGGA AATGATCAAG
    TCTCGCCAGA AAAAGGATTC TCGATTCCAT ACTTTTGTGC AAGACGCTGA GAGTAACCCC
    CTGTGCCGTC GTCTTCAGCT GAAGGACATC ATTCCCACCC AAATGCAGAG ACTCACTAAG
    TACCCGCTTC TTTTGGATAA CATTGCCAAG TACACAGAAT GGCCTCCAGA AAGGGAAAAG
    GTGAAGAAGG CTGCAGACCA TTGTCGTCAG ATCTTAAATT ATGTAAATCA GGCTGTCAGG
    GAGGCAGAAA ACAAGCAGCG CTTAGAAGAC TATCAGCGTC GCCTTGACAC CTCCAATCTC
    AAGTTGTCCG AGTACCCAAA TGTTGACGAG CTCAGGAATT TGGATTTAAC AAAAAGAAAG
    ATGATTCATG AAGGGCCGTT GGTCTGGAAG GTGAATAGAG ATAAAAGCAT CGATCTTTAT
    ACACTGCTGC TGGAAGACAT CCTTGTGCTG CTACAGAAGC AGGATGACAG GCTGGTGCTA
    AGGTGCCACA GCAAGATTCT GGCATCCACA GCCGACAGCA AACACACCTT CAGCCCTGTC
    ATTAAGCTGA GCACGGTGCT GGTTCGGCAG GTGGCGACAG ATAACAAAGC TTTATTCGTC
    ATCTCCATGT CAGATAATGG AGCCCAAATT TATGAATTGG TAGCACAGAC GGTTTCTGAA
    AAGACAGTCT GGCAAGACCT CATCTGTCGA ATGGCTGCGT CAGTGAAGGA GCAGTCTACA
    AAGCCGATTC CCCTCCCACA GCCGCCACCT TGCGAAGGAG ACAATGATGA AGAAGAACCA
    GCCAAGTTAA AAGTGGAGCA CCATGATCTT TCAGTCGCTG GTCTGCAAAG TCCAGACAGA
    GTTTTGGGAT TAGAATCTCC CTTAATTTCG TCAAAGCCTC AGTCTCATTC ACTGAATACC
    CCTGGCAAAT CAGCGGCAGA ACATCTCTTT GTGACCGCAA CACAGTTTGC AAAGGAACAG
    CATGCAAATG GGGCACTCAA GGAAGGTGAT GGCGGGTATC CAGTCACAAT CCCAGGTCCA
    CACTTGCCTG TGTCAGAGGA GCGGTGGGCA TTGGACGCAC TGAGAAATTT GGGCTTGTTG
    AAGCAGTTGC TGGTACAGCA GTTAGGTCTC ACCGAGAAGA GTACTCAGGA AGACTGGCAG
    TCCTTCTCAC GATACGGGCC AGCTTCTGAA GAGGTACAGG CAGACAGTGG CATCCGGGAC
    TTGGAAAATG TTAAGGCTTG TCATGCCAGG GAAGGACAGA TGTCTTTTAA AACTGGAACT
    GGTGATATTG CAACTTGTGA CAGTCCACGG ACTTCAACTG AGTCTTGTGC TGCACAGGAT
    TCAGTGATAC TGGCATCCCA AGACAGCCAG GCAAGTAACG TTTTAGTAAT GGACCACATG
    ATCCTGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGG GTCTGGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAAAAGG AAGAGAAGGA TGTTAATTTA CGCATCTCAG GAAACTGTTT GATCCTTGAT
    GGCTATGATG CAGTTCAGGA GAGCTCCACA GATGAGGAGG TTGCCTCCTC GTTCCCCCTG
    CAGCCTGTGA CGGGCATCCC CGCTGTGGAC TCCAGCCACC AGCAGCAACA TTCCCCTCAG
    AATGTGCATC CCGAGGGGCC GGTTTCACCA TTCACCCCAG AATTCCTGGT CCAGCGGCAC
    TGGCGAGCTA TGGAGGATAC CTGTTTTGAG ATCCAGAGTC CCTCATGTAC AGATTCACAA
    AGCCAAATCC TGGAGTACAT TCATAAAATA GAGGCTGACC TGGAACACTT AAAGAAGGTG
    GAGGAAAGTT ACGCTCTTCT TTGCCAAAGG CTGGCTGGCT CAGCCCTCCC CGACAAGCTC
    TCAGATAAAA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu70444
    Clone ID Related Accession (Same CDS sequence) XM_011242598.2 , XM_011242598.3
    Accession Version XM_011242598.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product rho guanine nucleotide exchange factor 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011242598.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC 
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011240900.1
    CDS352..4482
    Translation

    Target ORF information:

    RefSeq Version XM_011242598.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus Rho guanine nucleotide exchange factor (GEF) 12 (Arhgef12), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011242598.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTCCCA TTATGACATC CCCTCATTCC CCTGGAGCAG CCGGGAATAT GGAGAGAATC 
    ACCAGTCCTG TGCTCGTGGG GGAGGAAAAC AATGTGGTCC ATAACCAGAA AGTAGAGATT
    CTGAGAAAGA TGTTACAGAA GGAACAGGAA CGGCTGCAGT TGCTGCAGGA AGATTACAAC
    CGAACGGCTA CCCAAAGATT GCTCAAGGAG ATCCAGGAGG CCAAGAAGCA CATCCCGCAG
    CTGCAGGAGC AGCTGTCCAA AGCCACGGGC TCTGCTCAGG ACGGAGCTGT AATTGCACCC
    TCCAGGCCAT TAGGTGATGC CCTAACACTC AGTGAGGCAG AAGCAGATCC TGGAGATGGC
    CTGTGCAGGA CTGACTGGAG CAGTGGAGAT GCTTCTCGGC CCAGTAGTGA CAGTGCTGAT
    AGTCCCAAGA GTAGCCTGAG AGAAAGGAGT TACCTGGAGG AAGCCCCAGA GAGAAGTGAA
    GGAGTTCAGG ACGCTCAGGA GCCGCAGTCG CTGGTCGGCA GTCCCTCCAC CCGGGGCGCC
    CCTCACATCA TTGGAGCAGA GGATGATGAT TTTGGTACTG AGCATGAGCA GATCAATGGA
    CAGTGCAGCT GTTTCCAGAG CATTGAATTG CTGAAGTCTC GCCCTGCTCA CTTGGCTGTT
    TTCTTACACC ATGTAGTCTC ACAGTTTGAC CCTGCAACAT TGCTCTGTTA TCTCTATTCA
    GACCTGTATA AGCAGACAAA CTCCAAGGAG ACTCGGCGTG TCTTCCTGGA GTTTCATCAG
    TTCTTCTTGG ACCGGTCTGC ACACCTGAAG GTTCCAGTTC CTGAGGAAAT CTCCGTGGAT
    CTAGAAAAGA GAAGACCTGA GCTTATTCCT GAGGATCTGC ATCGCCTCTA CATTCAGACA
    ATGCAGGAGA GAGTCCACCC TGAAGTTCAG AGGCACTTGG AGGATTTCCG ACAGAAACGC
    AGCATGGGGC TGACCTTGGC TGAGAGTGAG CTGACTAAAC TTGATGCAGA GAGAGACAAG
    GACCGGGGAA CTCTGGAGAA GGAGCGAGCA TGTGCGGAAC AGATCGTTAC CAAAATTGAA
    GAAGTGTTGA TGACTGCTCA GGCTGTAGAA GAAGAAAGGA GTTCCACAAT GCAGTATGTA
    ATTCTCATGT ATATGAAGTA TTTGGGAGTA AAAGTGAAAG AACCTCGAAA CTTGGAGCAC
    AAGCGGGGTC GGATCGGCTT CCTTCCAAAG ATCAAGCAAA GCATGAAGAA AGATAGAGAG
    GGCGAAGAAA AAGGGAAGCG AAGAGGATTC CCCAGCATTT TGGGACCCCC AAGAAGACCA
    AGCCGCCACG ACAACAGTGC AATTGGCAGG GCCATGGAGA TACAGAAGTC ACGCCACCCG
    AAGCACTTAT CCACTCCCTC GTCTGTGAGT CCTGAGCCTC AGGACCCTGC CAAGTTGCGC
    CAGAGTGGAG TAGCCAATGA AGGAACAGAC ACTGGATACC TGCCCGCCAG TTCCATGTCC
    TCTGCCACCT CAGGGACTGC TCTTTCCCAA GAGGGAGGGA GAGAGAATGA TACAGGAACA
    AAACAAGTGG GAGAAGCATC GGCGCCTGGA GACTGCTTGG ACAGCACACC TCGAGTCCCC
    ACTACTGTTT TCGACTTCCC TCCTCCTCTA TTAGATCAAG TGCAGGAGGA GGAGTGTGAA
    GTAGAGAGGG TGGCAGAACA TGGGACCCCA AAACCCTTTC GGAAGTTTGA CAGCATCGCC
    TTTGGAGAAA GTCAGAGTGA GGATGAGCAG TTTGAAAATG ACCTGGAGAC AGATCCACCC
    AACTGGCAGC AGCTTGTTAG CAGAGAAGTG TTACTGGGAC TGAAACCTTC TGAGATCAAA
    AGACAGGAAG TGATTAATGA ATTGTTCTAC ACTGAGAGAG CTCATGTCCG GACACTGAAG
    GTTCTTGACC AAGTATTCTA TCAGCGAGTG TCCAGAGAAG GGATTCTATC ACCTTCAGAA
    CTACGCAAGA TTTTTTCAAA CTTGGAAGAT ATCCTTCAAC TTCATGTTGG GTTGAATGAG
    CAAATGAAAG CTGTCCGAAA AAGGAATGAA ACTTCTGTTA TTGATCATAT TGGGGAAGAT
    CTGCTCATCT GGTTTAGTGG ACCAGGAGAG GAGAAGCTGA AACATGCTGC TGCCACATTC
    TGCAGTAACC AACCTTTCGC CCTGGAAATG ATCAAGTCTC GCCAGAAAAA GGATTCTCGA
    TTCCATACTT TTGTGCAAGA CGCTGAGAGT AACCCCCTGT GCCGTCGTCT TCAGCTGAAG
    GACATCATTC CCACCCAAAT GCAGAGACTC ACTAAGTACC CGCTTCTTTT GGATAACATT
    GCCAAGTACA CAGAATGGCC TCCAGAAAGG GAAAAGGTGA AGAAGGCTGC AGACCATTGT
    CGTCAGATCT TAAATTATGT AAATCAGGCT GTCAGGGAGG CAGAAAACAA GCAGCGCTTA
    GAAGACTATC AGCGTCGCCT TGACACCTCC AATCTCAAGT TGTCCGAGTA CCCAAATGTT
    GACGAGCTCA GGAATTTGGA TTTAACAAAA AGAAAGATGA TTCATGAAGG GCCGTTGGTC
    TGGAAGGTGA ATAGAGATAA AAGCATCGAT CTTTATACAC TGCTGCTGGA AGACATCCTT
    GTGCTGCTAC AGAAGCAGGA TGACAGGCTG GTGCTAAGGT GCCACAGCAA GATTCTGGCA
    TCCACAGCCG ACAGCAAACA CACCTTCAGC CCTGTCATTA AGCTGAGCAC GGTGCTGGTT
    CGGCAGGTGG CGACAGATAA CAAAGCTTTA TTCGTCATCT CCATGTCAGA TAATGGAGCC
    CAAATTTATG AATTGGTAGC ACAGACGGTT TCTGAAAAGA CAGTCTGGCA AGACCTCATC
    TGTCGAATGG CTGCGTCAGT GAAGGAGCAG TCTACAAAGC CGATTCCCCT CCCACAGCCG
    CCACCTTGCG AAGGAGACAA TGATGAAGAA GAACCAGCCA AGTTAAAAGT GGAGCACCAT
    GATCTTTCAG TCGCTGGTCT GCAAAGTCCA GACAGAGTTT TGGGATTAGA ATCTCCCTTA
    ATTTCGTCAA AGCCTCAGTC TCATTCACTG AATACCCCTG GCAAATCAGC GGCAGAACAT
    CTCTTTGTGA CCGCAACACA GTTTGCAAAG GAACAGCATG CAAATGGGGC ACTCAAGGAA
    GGTGATGGCG GGTATCCAGT CACAATCCCA GGTCCACACT TGCCTGTGTC AGAGGAGCGG
    TGGGCATTGG ACGCACTGAG AAATTTGGGC TTGTTGAAGC AGTTGCTGGT ACAGCAGTTA
    GGTCTCACCG AGAAGAGTAC TCAGGAAGAC TGGCAGTCCT TCTCACGATA CGGGCCAGCT
    TCTGAAGAGG TACAGGCAGA CAGTGGCATC CGGGACTTGG AAAATGTTAA GGCTTGTCAT
    GCCAGGGAAG GACAGATGTC TTTTAAAACT GGAACTGGTG ATATTGCAAC TTGTGACAGT
    CCACGGACTT CAACTGAGTC TTGTGCTGCA CAGGATTCAG TGATACTGGC ATCCCAAGAC
    AGCCAGGCAA GTAACGTTTT AGTAATGGAC CACATGATCC TGACCCCAGA GATGCCTCCT
    GCAGAGCCAG AAGGGGGTCT GGATGAGAGT GGAGAGCACT TTTTTGATGC CCGTGAAGCA
    CATAGTGATG ATAATCCATC AGAAGGTGAT GGAGCAGTTA AAAAGGAAGA GAAGGATGTT
    AATTTACGCA TCTCAGGAAA CTGTTTGATC CTTGATGGCT ATGATGCAGT TCAGGAGAGC
    TCCACAGATG AGGAGGTTGC CTCCTCGTTC CCCCTGCAGC CTGTGACGGG CATCCCCGCT
    GTGGACTCCA GCCACCAGCA GCAACATTCC CCTCAGAATG TGCATCCCGA GGGGCCGGTT
    TCACCATTCA CCCCAGAATT CCTGGTCCAG CGGCACTGGC GAGCTATGGA GGATACCTGT
    TTTGAGATCC AGAGTCCCTC ATGTACAGAT TCACAAAGCC AAATCCTGGA GTACATTCAT
    AAAATAGAGG CTGACCTGGA ACACTTAAAG AAGGTGGAGG AAAGTTACGC TCTTCTTTGC
    CAAAGGCTGG CTGGCTCAGC CCTCCCCGAC AAGCTCTCAG ATAAAAGTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.