ARHGEF12 cDNA ORF clone, Canis lupus familiaris(dog)

The following ARHGEF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe287914 XM_022418191.1
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe36303 XM_014113363.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe36304 XM_014113364.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe05839 XM_005619662.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe05840 XM_005619663.2
Latest version!
Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OCe05840 XM_005619663.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe05839 XM_005619662.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe36304 XM_014113364.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe36303 XM_014113363.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe287914
    Clone ID Related Accession (Same CDS sequence) XM_022418191.1
    Accession Version XM_022418191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4545bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTAGGAG ACAACGATGA AGAAGCAGCT CCAAAGTTAA AAGTGGAACA TCATAGCATT
    TCAGTCCCTG GTTTGCAGAG TCCAGACAGA GATTTGGGAT TAGAGTCTCC CTTAATATCA
    TCAAAACCTC AGTCTCATTC ACTGGGTACC TCTGGGAAAT CCGAGGTAGA TGATCTGTTT
    GTGGCCGAGA GGCAGTTTGC TAAGGAGCAG CATACAGATG GGACTCTGCA GGAAGCTGGA
    GGAAGTTATC AGATCACAAT CCCAGATCCA CACTTGCCTG CCTCCGAAGA ACGGTGGGCA
    CTGGATGCAC TAAGGAATTT GGGCTTGCTG AAGCAGTTGC TCGTCCAACA GCTGGGTTTG
    ACCGAGAAGA GTGCTCAGGA AGACTGGCCA CATTTCCCGA GATACAGGAC GGCGTCTTCA
    GGGGTGCAGA CAGACAGTGG AACCCAGAAC TCTGAAAACA GTAAGGCCTA TCCTCCTGGT
    GAAGGACAGA TGCCCTTTAG AACTGGAACT GGTGACATTT CAACTTGTTA CAGTCCACGG
    ACTTCCACTG AATCTTCTGC TCCACGGGAT TCAGTGGTCC TGGCATTCCA AGAGAGCCAG
    GCAAGTAACA TTTTAGTAAT GGACCACATG ATTATGACCC CAGAGATGCC TCCTGCAGAG
    CCAGAAGGGA GTCTTGATGA GAGTGGAGAG CACTTTTTTG ATGCCCGTGA AGCACATAGT
    GATGATAATC CATCAGAAGG TGATGGAGCA GTTAAGAAGG AAGAGGATGT TAATTTGCGC
    ATCTCAGGAA ACTATTTGAT TCTTGATGGC TATGAAGCGG TGCAGGAGAG CTCCACGGAC
    GAGGAGGTTG CTTCCTCGCT TCCCCCGCCG CCCACGGCAG GCATCCCCGC CATGGACTGT
    GCCCGCCCAC AGCAGCACTC TCCCCAGCAT GCCCATTCCG ATGGGGCGGC TTCACCATTC
    ACCCCAGAAT TCCTGGTCCA GCAGCGCTGG GGAGCTATGG AGGATTCCTG TTTTGAGATC
    CAGAGCCCCC CCTCTTGTGC AGACTCACAA AGCCAGATCA TGGAGTACAT TCGTAAGATC
    GAGGCTGACC TAGAACACCT AAAGAAGGTG GAAGAGAGTT ACAGCATTCT TTGCCAAAGG
    CTGGCTGGAT CAGCCCTCAC AGACAAGCAC TCAGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022273899.1
    CDS76..4620
    Translation

    Target ORF information:

    RefSeq Version XM_022418191.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTAGGAG ACAACGATGA AGAAGCAGCT CCAAAGTTAA AAGTGGAACA TCATAGCATT
    TCAGTCCCTG GTTTGCAGAG TCCAGACAGA GATTTGGGAT TAGAGTCTCC CTTAATATCA
    TCAAAACCTC AGTCTCATTC ACTGGGTACC TCTGGGAAAT CCGAGGTAGA TGATCTGTTT
    GTGGCCGAGA GGCAGTTTGC TAAGGAGCAG CATACAGATG GGACTCTGCA GGAAGCTGGA
    GGAAGTTATC AGATCACAAT CCCAGATCCA CACTTGCCTG CCTCCGAAGA ACGGTGGGCA
    CTGGATGCAC TAAGGAATTT GGGCTTGCTG AAGCAGTTGC TCGTCCAACA GCTGGGTTTG
    ACCGAGAAGA GTGCTCAGGA AGACTGGCCA CATTTCCCGA GATACAGGAC GGCGTCTTCA
    GGGGTGCAGA CAGACAGTGG AACCCAGAAC TCTGAAAACA GTAAGGCCTA TCCTCCTGGT
    GAAGGACAGA TGCCCTTTAG AACTGGAACT GGTGACATTT CAACTTGTTA CAGTCCACGG
    ACTTCCACTG AATCTTCTGC TCCACGGGAT TCAGTGGTCC TGGCATTCCA AGAGAGCCAG
    GCAAGTAACA TTTTAGTAAT GGACCACATG ATTATGACCC CAGAGATGCC TCCTGCAGAG
    CCAGAAGGGA GTCTTGATGA GAGTGGAGAG CACTTTTTTG ATGCCCGTGA AGCACATAGT
    GATGATAATC CATCAGAAGG TGATGGAGCA GTTAAGAAGG AAGAGGATGT TAATTTGCGC
    ATCTCAGGAA ACTATTTGAT TCTTGATGGC TATGAAGCGG TGCAGGAGAG CTCCACGGAC
    GAGGAGGTTG CTTCCTCGCT TCCCCCGCCG CCCACGGCAG GCATCCCCGC CATGGACTGT
    GCCCGCCCAC AGCAGCACTC TCCCCAGCAT GCCCATTCCG ATGGGGCGGC TTCACCATTC
    ACCCCAGAAT TCCTGGTCCA GCAGCGCTGG GGAGCTATGG AGGATTCCTG TTTTGAGATC
    CAGAGCCCCC CCTCTTGTGC AGACTCACAA AGCCAGATCA TGGAGTACAT TCGTAAGATC
    GAGGCTGACC TAGAACACCT AAAGAAGGTG GAAGAGAGTT ACAGCATTCT TTGCCAAAGG
    CTGGCTGGAT CAGCCCTCAC AGACAAGCAC TCAGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36303
    Clone ID Related Accession (Same CDS sequence) XM_014113363.1 , XM_014113363.2
    Accession Version XM_014113363.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGGTCTGGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGGGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT CGCTGATTCT
    GAAGTAGAGC CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT
    GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAGATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACCTCCTCC AAGCCTGTAG GTGAGCCGCT GACAGCCAGT
    GAGGTAGAAG CAGATCCTGG TGAGGGAATA GGCAGACTTG ACTACTGCAC TGGAGATGCT
    TCTCGGCCCG GCAGTGACAG CGCTGACAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTGT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAGTG ATTCAGGACA CTGATACCCA GTCTCTCTTT
    GGGAGCCCCT CTCCCCGTAT AGCAGCTCAT ATTATTGGAG CAGAGGATGA TGATTTCGGT
    ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTGAAA
    TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCCGCC
    ACACTGCTTT GTTATCTGTA TTCAGACCTG TATAAGCAGA CCAATTCCAA GGAGACTCGT
    CGCGTCTTCC TTGAGTTTCA TCAGTTCTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT
    GTGCCTGATG AAATATCAGT AGATCTAGAA AAAAGGAGAC CTGAGCTTAT ACCTGAGGAT
    CTACACCGCC ACTATATCCA AACAATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC
    TTGGAGGATT TTCGGCAGAA GCGTAGCATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTCGACG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCA
    GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGCCTGT GGAAGAAGAT
    AAGAGTTCAA CAATGCAGTA TGTTATCCTA ATGTACATGA AGCATTTGGG GGTAAAAGTG
    AAAGAACCTC GAAATTTGGA GCACAAGCGG GGTCGGATTG GATTCCTTCC AAAAATCAAG
    CAGAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGAA AGCGCAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CACGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGCAGCGCCA CCCGAAGCAC TTATCCACAC CTTCTTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GCATTAGCAG GCGAAGGAAC AGAGGTCGGA
    TACCTGCCAG CCAATCCCAT GTCCTCTGTG GCTGCTGGGG CCACTCTTTC CCAGGAAGGA
    GGGAAGGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CACCAGCATC TGGAGACTGC
    ATAGATAATA CCCCTCGGAC TCCCAATACC ATCTTTGATT TCCCACCTCC TCCCTTAGAC
    CAAGTGCAGG AGGAGGAATG GGAAGTGGAA AGGGTGACTG AGCATGGGAC ACCAAAACCC
    TTTCGAAAGT TTGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG
    GGCCTAAAGC CTTCTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TCCGAACACT AAAGGTTCTT GATCAAGTAT TCTATCAGCG AGTGTCCAGG
    GAAGGAATTC TGTCCCCTTC AGAACTACGG AAGATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCTGTTC GAAAGAGGAA TGAGACCTCA
    GTTATTGATC AGATTGGGGA AGATTTGCTG ACCTGGTTCA GTGGACCAGG GGAGGACAAG
    CTGAAGCACG CCGCTGCTAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG
    TCTCGTCAGA AAAAGGATTC TCGATTCCAG ACCTTTGTGC AAGATGCTGA GAGTAATCCT
    CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AGATGCAGAG GCTTACCAAG
    TACCCTCTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG
    GTGAAGAAAG CCGCAGATCA CTGTCGTCAG ATCTTAAATT ACGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAACAGCG ACTAGAAGAC TACCAGCGTC GCCTGGATAC CTCCAATCTG
    AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC GAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTCTGGAAG GTGAATCGAG ATAAAACTAT TGATCTATAC
    ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTGTTA
    AGATGTCATA GTAAGATTCT GGCATCCACG GCTGACAGCA AACACACATT TAGCCCTGTC
    ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTTGTC
    ATCTCCATGT CAGACAATGG AGCCCAGATT TATGAACTGG TGGCACAGAC TGTTTCTGAA
    AAGACTGTCT GGCAAGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA ACAGTCCGCA
    AAGCCAATCC CATTGCCACA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGCAGCT
    CCAAAGTTAA AAGTGGAACA TCATAGCATT TCAGTCCCTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAGTCTCC CTTAATATCA TCAAAACCTC AGTCTCATTC ACTGGGTACC
    TCTGGGAAAT CCGAGGTAGA TGATCTGTTT GTGGCCGAGA GGCAGTTTGC TAAGGAGCAG
    CATACAGATG GGACTCTGCA GGAAGCTGGA GGAAGTTATC AGATCACAAT CCCAGATCCA
    CACTTGCCTG CCTCCGAAGA ACGGTGGGCA CTGGATGCAC TAAGGAATTT GGGCTTGCTG
    AAGCAGTTGC TCGTCCAACA GCTGGGTTTG ACCGAGAAGA GTGCTCAGGA AGACTGGCCA
    CATTTCCCGA GATACAGGAC GGCGTCTTCA GGGGTGCAGA CAGACAGTGG AACCCAGAAC
    TCTGAAAACA GTAAGGCCTA TCCTCCTGGT GAAGGACAGA TGCCCTTTAG AACTGGAACT
    GGTGACATTT CAACTTGTTA CAGTCCACGG ACTTCCACTG AATCTTCTGC TCCACGGGAT
    TCAGTGGTCC TGGCATTCCA AGAGAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT TCTTGATGGC
    TATGAAGCGG TGCAGGAGAG CTCCACGGAC GAGGAGGTTG CTTCCTCGCT TCCCCCGCCG
    CCCACGGCAG GCATCCCCGC CATGGACTGT GCCCGCCCAC AGCAGCACTC TCCCCAGCAT
    GCCCATTCCG ATGGGGCGGC TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGCCCCC CCTCTTGTGC AGACTCACAA
    AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TAGAACACCT AAAGAAGGTG
    GAAGAGAGTT ACAGCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968838.1
    CDS70..4644
    Translation

    Target ORF information:

    RefSeq Version XM_014113363.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGGTCTGGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGGGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT CGCTGATTCT
    GAAGTAGAGC CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT
    GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAGATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACCTCCTCC AAGCCTGTAG GTGAGCCGCT GACAGCCAGT
    GAGGTAGAAG CAGATCCTGG TGAGGGAATA GGCAGACTTG ACTACTGCAC TGGAGATGCT
    TCTCGGCCCG GCAGTGACAG CGCTGACAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTGT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAGTG ATTCAGGACA CTGATACCCA GTCTCTCTTT
    GGGAGCCCCT CTCCCCGTAT AGCAGCTCAT ATTATTGGAG CAGAGGATGA TGATTTCGGT
    ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTGAAA
    TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCCGCC
    ACACTGCTTT GTTATCTGTA TTCAGACCTG TATAAGCAGA CCAATTCCAA GGAGACTCGT
    CGCGTCTTCC TTGAGTTTCA TCAGTTCTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT
    GTGCCTGATG AAATATCAGT AGATCTAGAA AAAAGGAGAC CTGAGCTTAT ACCTGAGGAT
    CTACACCGCC ACTATATCCA AACAATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC
    TTGGAGGATT TTCGGCAGAA GCGTAGCATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTCGACG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCA
    GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGCCTGT GGAAGAAGAT
    AAGAGTTCAA CAATGCAGTA TGTTATCCTA ATGTACATGA AGCATTTGGG GGTAAAAGTG
    AAAGAACCTC GAAATTTGGA GCACAAGCGG GGTCGGATTG GATTCCTTCC AAAAATCAAG
    CAGAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGAA AGCGCAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CACGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGCAGCGCCA CCCGAAGCAC TTATCCACAC CTTCTTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GCATTAGCAG GCGAAGGAAC AGAGGTCGGA
    TACCTGCCAG CCAATCCCAT GTCCTCTGTG GCTGCTGGGG CCACTCTTTC CCAGGAAGGA
    GGGAAGGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CACCAGCATC TGGAGACTGC
    ATAGATAATA CCCCTCGGAC TCCCAATACC ATCTTTGATT TCCCACCTCC TCCCTTAGAC
    CAAGTGCAGG AGGAGGAATG GGAAGTGGAA AGGGTGACTG AGCATGGGAC ACCAAAACCC
    TTTCGAAAGT TTGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG
    GGCCTAAAGC CTTCTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TCCGAACACT AAAGGTTCTT GATCAAGTAT TCTATCAGCG AGTGTCCAGG
    GAAGGAATTC TGTCCCCTTC AGAACTACGG AAGATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCTGTTC GAAAGAGGAA TGAGACCTCA
    GTTATTGATC AGATTGGGGA AGATTTGCTG ACCTGGTTCA GTGGACCAGG GGAGGACAAG
    CTGAAGCACG CCGCTGCTAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG
    TCTCGTCAGA AAAAGGATTC TCGATTCCAG ACCTTTGTGC AAGATGCTGA GAGTAATCCT
    CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AGATGCAGAG GCTTACCAAG
    TACCCTCTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG
    GTGAAGAAAG CCGCAGATCA CTGTCGTCAG ATCTTAAATT ACGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAACAGCG ACTAGAAGAC TACCAGCGTC GCCTGGATAC CTCCAATCTG
    AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC GAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTCTGGAAG GTGAATCGAG ATAAAACTAT TGATCTATAC
    ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTGTTA
    AGATGTCATA GTAAGATTCT GGCATCCACG GCTGACAGCA AACACACATT TAGCCCTGTC
    ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTTGTC
    ATCTCCATGT CAGACAATGG AGCCCAGATT TATGAACTGG TGGCACAGAC TGTTTCTGAA
    AAGACTGTCT GGCAAGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA ACAGTCCGCA
    AAGCCAATCC CATTGCCACA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGCAGCT
    CCAAAGTTAA AAGTGGAACA TCATAGCATT TCAGTCCCTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAGTCTCC CTTAATATCA TCAAAACCTC AGTCTCATTC ACTGGGTACC
    TCTGGGAAAT CCGAGGTAGA TGATCTGTTT GTGGCCGAGA GGCAGTTTGC TAAGGAGCAG
    CATACAGATG GGACTCTGCA GGAAGCTGGA GGAAGTTATC AGATCACAAT CCCAGATCCA
    CACTTGCCTG CCTCCGAAGA ACGGTGGGCA CTGGATGCAC TAAGGAATTT GGGCTTGCTG
    AAGCAGTTGC TCGTCCAACA GCTGGGTTTG ACCGAGAAGA GTGCTCAGGA AGACTGGCCA
    CATTTCCCGA GATACAGGAC GGCGTCTTCA GGGGTGCAGA CAGACAGTGG AACCCAGAAC
    TCTGAAAACA GTAAGGCCTA TCCTCCTGGT GAAGGACAGA TGCCCTTTAG AACTGGAACT
    GGTGACATTT CAACTTGTTA CAGTCCACGG ACTTCCACTG AATCTTCTGC TCCACGGGAT
    TCAGTGGTCC TGGCATTCCA AGAGAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT TCTTGATGGC
    TATGAAGCGG TGCAGGAGAG CTCCACGGAC GAGGAGGTTG CTTCCTCGCT TCCCCCGCCG
    CCCACGGCAG GCATCCCCGC CATGGACTGT GCCCGCCCAC AGCAGCACTC TCCCCAGCAT
    GCCCATTCCG ATGGGGCGGC TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGCCCCC CCTCTTGTGC AGACTCACAA
    AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TAGAACACCT AAAGAAGGTG
    GAAGAGAGTT ACAGCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36303
    Clone ID Related Accession (Same CDS sequence) XM_014113363.1 , XM_014113363.2
    Accession Version XM_014113363.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 12 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGGTCTGGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGGGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT CGCTGATTCT
    GAAGTAGAGC CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT
    GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAGATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACCTCCTCC AAGCCTGTAG GTGAGCCGCT GACAGCCAGT
    GAGGTAGAAG CAGATCCTGG TGAGGGAATA GGCAGACTTG ACTACTGCAC TGGAGATGCT
    TCTCGGCCCG GCAGTGACAG CGCTGACAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTGT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAGTG ATTCAGGACA CTGATACCCA GTCTCTCTTT
    GGGAGCCCCT CTCCCCGTAT AGCAGCTCAT ATTATTGGAG CAGAGGATGA TGATTTCGGT
    ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTGAAA
    TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCCGCC
    ACACTGCTTT GTTATCTGTA TTCAGACCTG TATAAGCAGA CCAATTCCAA GGAGACTCGT
    CGCGTCTTCC TTGAGTTTCA TCAGTTCTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT
    GTGCCTGATG AAATATCAGT AGATCTAGAA AAAAGGAGAC CTGAGCTTAT ACCTGAGGAT
    CTACACCGCC ACTATATCCA AACAATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC
    TTGGAGGATT TTCGGCAGAA GCGTAGCATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTCGACG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCA
    GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGCCTGT GGAAGAAGAT
    AAGAGTTCAA CAATGCAGTA TGTTATCCTA ATGTACATGA AGCATTTGGG GGTAAAAGTG
    AAAGAACCTC GAAATTTGGA GCACAAGCGG GGTCGGATTG GATTCCTTCC AAAAATCAAG
    CAGAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGAA AGCGCAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CACGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGCAGCGCCA CCCGAAGCAC TTATCCACAC CTTCTTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GCATTAGCAG GCGAAGGAAC AGAGGTCGGA
    TACCTGCCAG CCAATCCCAT GTCCTCTGTG GCTGCTGGGG CCACTCTTTC CCAGGAAGGA
    GGGAAGGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CACCAGCATC TGGAGACTGC
    ATAGATAATA CCCCTCGGAC TCCCAATACC ATCTTTGATT TCCCACCTCC TCCCTTAGAC
    CAAGTGCAGG AGGAGGAATG GGAAGTGGAA AGGGTGACTG AGCATGGGAC ACCAAAACCC
    TTTCGAAAGT TTGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG
    GGCCTAAAGC CTTCTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TCCGAACACT AAAGGTTCTT GATCAAGTAT TCTATCAGCG AGTGTCCAGG
    GAAGGAATTC TGTCCCCTTC AGAACTACGG AAGATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCTGTTC GAAAGAGGAA TGAGACCTCA
    GTTATTGATC AGATTGGGGA AGATTTGCTG ACCTGGTTCA GTGGACCAGG GGAGGACAAG
    CTGAAGCACG CCGCTGCTAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG
    TCTCGTCAGA AAAAGGATTC TCGATTCCAG ACCTTTGTGC AAGATGCTGA GAGTAATCCT
    CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AGATGCAGAG GCTTACCAAG
    TACCCTCTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG
    GTGAAGAAAG CCGCAGATCA CTGTCGTCAG ATCTTAAATT ACGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAACAGCG ACTAGAAGAC TACCAGCGTC GCCTGGATAC CTCCAATCTG
    AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC GAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTCTGGAAG GTGAATCGAG ATAAAACTAT TGATCTATAC
    ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTGTTA
    AGATGTCATA GTAAGATTCT GGCATCCACG GCTGACAGCA AACACACATT TAGCCCTGTC
    ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTTGTC
    ATCTCCATGT CAGACAATGG AGCCCAGATT TATGAACTGG TGGCACAGAC TGTTTCTGAA
    AAGACTGTCT GGCAAGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA ACAGTCCGCA
    AAGCCAATCC CATTGCCACA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGCAGCT
    CCAAAGTTAA AAGTGGAACA TCATAGCATT TCAGTCCCTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAGTCTCC CTTAATATCA TCAAAACCTC AGTCTCATTC ACTGGGTACC
    TCTGGGAAAT CCGAGGTAGA TGATCTGTTT GTGGCCGAGA GGCAGTTTGC TAAGGAGCAG
    CATACAGATG GGACTCTGCA GGAAGCTGGA GGAAGTTATC AGATCACAAT CCCAGATCCA
    CACTTGCCTG CCTCCGAAGA ACGGTGGGCA CTGGATGCAC TAAGGAATTT GGGCTTGCTG
    AAGCAGTTGC TCGTCCAACA GCTGGGTTTG ACCGAGAAGA GTGCTCAGGA AGACTGGCCA
    CATTTCCCGA GATACAGGAC GGCGTCTTCA GGGGTGCAGA CAGACAGTGG AACCCAGAAC
    TCTGAAAACA GTAAGGCCTA TCCTCCTGGT GAAGGACAGA TGCCCTTTAG AACTGGAACT
    GGTGACATTT CAACTTGTTA CAGTCCACGG ACTTCCACTG AATCTTCTGC TCCACGGGAT
    TCAGTGGTCC TGGCATTCCA AGAGAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT TCTTGATGGC
    TATGAAGCGG TGCAGGAGAG CTCCACGGAC GAGGAGGTTG CTTCCTCGCT TCCCCCGCCG
    CCCACGGCAG GCATCCCCGC CATGGACTGT GCCCGCCCAC AGCAGCACTC TCCCCAGCAT
    GCCCATTCCG ATGGGGCGGC TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGCCCCC CCTCTTGTGC AGACTCACAA
    AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TAGAACACCT AAAGAAGGTG
    GAAGAGAGTT ACAGCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968838.1
    CDS76..4650
    Translation

    Target ORF information:

    RefSeq Version XM_014113363.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113363.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGGTCTGGTT CAGCGTTGTG TGATCATCCA GAAGGATGAC
    AATGGATTTG GGCTGACGGT CAGTGGAGAC AATCCAGTCT TCGTACAGTC TGTCAAAGAA
    GATGGAGCAG CCATGCGGGC TGGGGTACAG ACAGGTGATC GAATCATCAA GGTGAATGGA
    ACTCTGGTGA CTCATTCAAA CCATTTGGAA GTGGTGAAGC TAATCAAATC TGGTTCCTAT
    GTAGCTCTCA CTGTTCAGGG ACGCCCACCT GGGTCGCCCC AGATTCCACT CGCTGATTCT
    GAAGTAGAGC CATCAGTCAT TGGACATATG TCTCCCATCA TGACATCCCC TCATTCACCT
    GGAGCATCTG GGAACATGGA GAGAATTACT AGTCCTGTGC TCATGGGGGA GGAAAACAAT
    GTGGTTCATA ACCAGAAAGT AGAAATTCTG AGAAAGATGT TACAGAAAGA ACAGGAACGG
    CTACAGTTAT TGCAGGAAGA TTACAACCGA ACACCTGCCC AAAGATTGCT AAAAGAGATC
    CAAGAGGCCA AGAAACACAT TCCTCAGCTG CAAGAGCAGT TATCCAAAGC CACAGGCTCT
    GCTCAGGATG GAGCTGTAGT TACCTCCTCC AAGCCTGTAG GTGAGCCGCT GACAGCCAGT
    GAGGTAGAAG CAGATCCTGG TGAGGGAATA GGCAGACTTG ACTACTGCAC TGGAGATGCT
    TCTCGGCCCG GCAGTGACAG CGCTGACAGT CCCAAGAGTG CCCTGAAAGA GAGGATTTGT
    CTAGAGGAAA ACCCAGAGAA AAGTGAAGTG ATTCAGGACA CTGATACCCA GTCTCTCTTT
    GGGAGCCCCT CTCCCCGTAT AGCAGCTCAT ATTATTGGAG CAGAGGATGA TGATTTCGGT
    ACTGAACATG AACAGATCAA TGGGCAGTGC AGCTGTTTCC AGAGCATTGA ACTGCTGAAA
    TCTCGCCCTG CTCACCTGGC TGTTTTCTTA CACCATGTAG TCTCACAGTT TGACCCCGCC
    ACACTGCTTT GTTATCTGTA TTCAGACCTG TATAAGCAGA CCAATTCCAA GGAGACTCGT
    CGCGTCTTCC TTGAGTTTCA TCAGTTCTTC CTGGATCGAT CTGCACACCT GAAAGTTTCT
    GTGCCTGATG AAATATCAGT AGATCTAGAA AAAAGGAGAC CTGAGCTTAT ACCTGAGGAT
    CTACACCGCC ACTATATCCA AACAATGCAG GAAAGAGTCC ACCCAGAAGT TCAGAGGCAC
    TTGGAGGATT TTCGGCAGAA GCGTAGCATG GGACTGACCT TGGCTGAAAG CGAGCTGACT
    AAACTCGACG CAGAGCGAGA CAAGGACCGG GTAACTTTGG AGAAGGAACG GGCATGTGCA
    GAACAGATTG TTGCCAAAAT CGAAGAAGTG TTGATGACTG CTCAGCCTGT GGAAGAAGAT
    AAGAGTTCAA CAATGCAGTA TGTTATCCTA ATGTACATGA AGCATTTGGG GGTAAAAGTG
    AAAGAACCTC GAAATTTGGA GCACAAGCGG GGTCGGATTG GATTCCTTCC AAAAATCAAG
    CAGAGTATGA AGAAAGATCG AGAAGGCGAA GAAAAAGGAA AGCGCAGAGG ATTCCCCAGC
    ATCCTGGGAC CCCCAAGGAG ACCAAGCCGT CACGACAACA GTGCAATTGG CAGAGCCATG
    GAACTACAGA AGCAGCGCCA CCCGAAGCAC TTATCCACAC CTTCTTCTGT GAGTCCTGAG
    CCTCAGGACC CTGCCAAGTT GCGCCAGAGT GCATTAGCAG GCGAAGGAAC AGAGGTCGGA
    TACCTGCCAG CCAATCCCAT GTCCTCTGTG GCTGCTGGGG CCACTCTTTC CCAGGAAGGA
    GGGAAGGAGA ATGATACAGG ATCAAAGCAA GTTGGAGAAA CACCAGCATC TGGAGACTGC
    ATAGATAATA CCCCTCGGAC TCCCAATACC ATCTTTGATT TCCCACCTCC TCCCTTAGAC
    CAAGTGCAGG AGGAGGAATG GGAAGTGGAA AGGGTGACTG AGCATGGGAC ACCAAAACCC
    TTTCGAAAGT TTGACAGCAT AGCTTTTGGA GAAAGTCAAA GTGAGGATGA ACAGTTTGAA
    AATGACTTAG AGACAGATCC ACCCAACTGG CAACAGCTCG TTAGTCGAGA AGTGTTACTG
    GGCCTAAAGC CTTCTGAGAT CAAAAGACAG GAAGTAATTA ATGAATTGTT CTACACTGAA
    AGAGCTCATG TCCGAACACT AAAGGTTCTT GATCAAGTAT TCTATCAGCG AGTGTCCAGG
    GAAGGAATTC TGTCCCCTTC AGAACTACGG AAGATTTTTT CAAACTTGGA AGATATTCTT
    CAACTTCACA TTGGATTGAA TGAGCAAATG AAGGCTGTTC GAAAGAGGAA TGAGACCTCA
    GTTATTGATC AGATTGGGGA AGATTTGCTG ACCTGGTTCA GTGGACCAGG GGAGGACAAG
    CTGAAGCACG CCGCTGCTAC CTTTTGCAGT AACCAACCTT TTGCCCTGGA AATGATCAAG
    TCTCGTCAGA AAAAGGATTC TCGATTCCAG ACCTTTGTGC AAGATGCTGA GAGTAATCCT
    CTGTGTCGTC GTCTGCAGCT GAAGGATATC ATTCCTACCC AGATGCAGAG GCTTACCAAG
    TACCCTCTTC TGTTGGATAA TATTGCCAAA TACACAGAAT GGCCAATGGA AAGGGAGAAG
    GTGAAGAAAG CCGCAGATCA CTGTCGTCAG ATCTTAAATT ACGTAAATCA GGCTGTCAAG
    GAGGCAGAAA ACAAACAGCG ACTAGAAGAC TACCAGCGTC GCCTGGATAC CTCCAATCTG
    AAGTTGTCAG AGTACCCAAA TGTGGAAGAG CTCAGGAATT TGGATTTAAC GAAAAGGAAG
    ATGATTCATG AAGGGCCATT GGTCTGGAAG GTGAATCGAG ATAAAACTAT TGATCTATAC
    ACATTGCTGC TGGAAGACAT TCTTGTATTG TTACAAAAGC AGGATGATAG ACTGGTGTTA
    AGATGTCATA GTAAGATTCT GGCATCCACG GCTGACAGCA AACACACATT TAGCCCTGTC
    ATTAAGTTGA ATACAGTGTT GGTTCGACAA GTGGCAACAG ATAACAAAGC TTTATTTGTC
    ATCTCCATGT CAGACAATGG AGCCCAGATT TATGAACTGG TGGCACAGAC TGTTTCTGAA
    AAGACTGTCT GGCAAGACCT AATCTGTCGG ATGGCTGCAT CAGTGAAGGA ACAGTCCGCA
    AAGCCAATCC CATTGCCACA GTCACCACCT TGCGAAGGAG ACAACGATGA AGAAGCAGCT
    CCAAAGTTAA AAGTGGAACA TCATAGCATT TCAGTCCCTG GTTTGCAGAG TCCAGACAGA
    GATTTGGGAT TAGAGTCTCC CTTAATATCA TCAAAACCTC AGTCTCATTC ACTGGGTACC
    TCTGGGAAAT CCGAGGTAGA TGATCTGTTT GTGGCCGAGA GGCAGTTTGC TAAGGAGCAG
    CATACAGATG GGACTCTGCA GGAAGCTGGA GGAAGTTATC AGATCACAAT CCCAGATCCA
    CACTTGCCTG CCTCCGAAGA ACGGTGGGCA CTGGATGCAC TAAGGAATTT GGGCTTGCTG
    AAGCAGTTGC TCGTCCAACA GCTGGGTTTG ACCGAGAAGA GTGCTCAGGA AGACTGGCCA
    CATTTCCCGA GATACAGGAC GGCGTCTTCA GGGGTGCAGA CAGACAGTGG AACCCAGAAC
    TCTGAAAACA GTAAGGCCTA TCCTCCTGGT GAAGGACAGA TGCCCTTTAG AACTGGAACT
    GGTGACATTT CAACTTGTTA CAGTCCACGG ACTTCCACTG AATCTTCTGC TCCACGGGAT
    TCAGTGGTCC TGGCATTCCA AGAGAGCCAG GCAAGTAACA TTTTAGTAAT GGACCACATG
    ATTATGACCC CAGAGATGCC TCCTGCAGAG CCAGAAGGGA GTCTTGATGA GAGTGGAGAG
    CACTTTTTTG ATGCCCGTGA AGCACATAGT GATGATAATC CATCAGAAGG TGATGGAGCA
    GTTAAGAAGG AAGAGGATGT TAATTTGCGC ATCTCAGGAA ACTATTTGAT TCTTGATGGC
    TATGAAGCGG TGCAGGAGAG CTCCACGGAC GAGGAGGTTG CTTCCTCGCT TCCCCCGCCG
    CCCACGGCAG GCATCCCCGC CATGGACTGT GCCCGCCCAC AGCAGCACTC TCCCCAGCAT
    GCCCATTCCG ATGGGGCGGC TTCACCATTC ACCCCAGAAT TCCTGGTCCA GCAGCGCTGG
    GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGCCCCC CCTCTTGTGC AGACTCACAA
    AGCCAGATCA TGGAGTACAT TCGTAAGATC GAGGCTGACC TAGAACACCT AAAGAAGGTG
    GAAGAGAGTT ACAGCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC
    TCAGATAAGA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36304
    Clone ID Related Accession (Same CDS sequence) XM_014113364.2 , XM_014113364.1
    Accession Version XM_014113364.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 12 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113364.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968839.1
    CDS76..4587
    Translation

    Target ORF information:

    RefSeq Version XM_014113364.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113364.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36304
    Clone ID Related Accession (Same CDS sequence) XM_014113364.2 , XM_014113364.1
    Accession Version XM_014113364.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 12 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013968839.1
    CDS70..4581
    Translation

    Target ORF information:

    RefSeq Version XM_014113364.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05839
    Clone ID Related Accession (Same CDS sequence) XM_005619662.2 , XM_005619662.1
    Accession Version XM_005619662.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619719.1
    CDS76..4707
    Translation

    Target ORF information:

    RefSeq Version XM_005619662.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05839
    Clone ID Related Accession (Same CDS sequence) XM_005619662.2 , XM_005619662.1
    Accession Version XM_005619662.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4632bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 12 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619719.1
    CDS70..4701
    Translation

    Target ORF information:

    RefSeq Version XM_005619662.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619662.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAG
    AGTATGAAGA AAGATCGAGA AGGCGAAGAA AAAGGAAAGC GCAGAGGATT CCCCAGCATC
    CTGGGACCCC CAAGGAGACC AAGCCGTCAC GACAACAGTG CAATTGGCAG AGCCATGGAA
    CTACAGAAGC AGCGCCACCC GAAGCACTTA TCCACACCTT CTTCTGTGAG TCCTGAGCCT
    CAGGACCCTG CCAAGTTGCG CCAGAGTGCA TTAGCAGGCG AAGGAACAGA GGTCGGATAC
    CTGCCAGCCA ATCCCATGTC CTCTGTGGCT GCTGGGGCCA CTCTTTCCCA GGAAGGAGGG
    AAGGAGAATG ATACAGGATC AAAGCAAGTT GGAGAAACAC CAGCATCTGG AGACTGCATA
    GATAATACCC CTCGGACTCC CAATACCATC TTTGATTTCC CACCTCCTCC CTTAGACCAA
    GTGCAGGAGG AGGAATGGGA AGTGGAAAGG GTGACTGAGC ATGGGACACC AAAACCCTTT
    CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAAAGTG AGGATGAACA GTTTGAAAAT
    GACTTAGAGA CAGATCCACC CAACTGGCAA CAGCTCGTTA GTCGAGAAGT GTTACTGGGC
    CTAAAGCCTT CTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA
    GCTCATGTCC GAACACTAAA GGTTCTTGAT CAAGTATTCT ATCAGCGAGT GTCCAGGGAA
    GGAATTCTGT CCCCTTCAGA ACTACGGAAG ATTTTTTCAA ACTTGGAAGA TATTCTTCAA
    CTTCACATTG GATTGAATGA GCAAATGAAG GCTGTTCGAA AGAGGAATGA GACCTCAGTT
    ATTGATCAGA TTGGGGAAGA TTTGCTGACC TGGTTCAGTG GACCAGGGGA GGACAAGCTG
    AAGCACGCCG CTGCTACCTT TTGCAGTAAC CAACCTTTTG CCCTGGAAAT GATCAAGTCT
    CGTCAGAAAA AGGATTCTCG ATTCCAGACC TTTGTGCAAG ATGCTGAGAG TAATCCTCTG
    TGTCGTCGTC TGCAGCTGAA GGATATCATT CCTACCCAGA TGCAGAGGCT TACCAAGTAC
    CCTCTTCTGT TGGATAATAT TGCCAAATAC ACAGAATGGC CAATGGAAAG GGAGAAGGTG
    AAGAAAGCCG CAGATCACTG TCGTCAGATC TTAAATTACG TAAATCAGGC TGTCAAGGAG
    GCAGAAAACA AACAGCGACT AGAAGACTAC CAGCGTCGCC TGGATACCTC CAATCTGAAG
    TTGTCAGAGT ACCCAAATGT GGAAGAGCTC AGGAATTTGG ATTTAACGAA AAGGAAGATG
    ATTCATGAAG GGCCATTGGT CTGGAAGGTG AATCGAGATA AAACTATTGA TCTATACACA
    TTGCTGCTGG AAGACATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGA
    TGTCATAGTA AGATTCTGGC ATCCACGGCT GACAGCAAAC ACACATTTAG CCCTGTCATT
    AAGTTGAATA CAGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTTGTCATC
    TCCATGTCAG ACAATGGAGC CCAGATTTAT GAACTGGTGG CACAGACTGT TTCTGAAAAG
    ACTGTCTGGC AAGACCTAAT CTGTCGGATG GCTGCATCAG TGAAGGAACA GTCCGCAAAG
    CCAATCCCAT TGCCACAGTC ACCACCTTGC GAAGGAGACA ACGATGAAGA AGCAGCTCCA
    AAGTTAAAAG TGGAACATCA TAGCATTTCA GTCCCTGGTT TGCAGAGTCC AGACAGAGAT
    TTGGGATTAG AGTCTCCCTT AATATCATCA AAACCTCAGT CTCATTCACT GGGTACCTCT
    GGGAAATCCG AGGTAGATGA TCTGTTTGTG GCCGAGAGGC AGTTTGCTAA GGAGCAGCAT
    ACAGATGGGA CTCTGCAGGA AGCTGGAGGA AGTTATCAGA TCACAATCCC AGATCCACAC
    TTGCCTGCCT CCGAAGAACG GTGGGCACTG GATGCACTAA GGAATTTGGG CTTGCTGAAG
    CAGTTGCTCG TCCAACAGCT GGGTTTGACC GAGAAGAGTG CTCAGGAAGA CTGGCCACAT
    TTCCCGAGAT ACAGGACGGC GTCTTCAGGG GTGCAGACAG ACAGTGGAAC CCAGAACTCT
    GAAAACAGTA AGGCCTATCC TCCTGGTGAA GGACAGATGC CCTTTAGAAC TGGAACTGGT
    GACATTTCAA CTTGTTACAG TCCACGGACT TCCACTGAAT CTTCTGCTCC ACGGGATTCA
    GTGGTCCTGG CATTCCAAGA GAGCCAGGCA AGTAACATTT TAGTAATGGA CCACATGATT
    ATGACCCCAG AGATGCCTCC TGCAGAGCCA GAAGGGAGTC TTGATGAGAG TGGAGAGCAC
    TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT
    AAGAAGGAAG AGGATGTTAA TTTGCGCATC TCAGGAAACT ATTTGATTCT TGATGGCTAT
    GAAGCGGTGC AGGAGAGCTC CACGGACGAG GAGGTTGCTT CCTCGCTTCC CCCGCCGCCC
    ACGGCAGGCA TCCCCGCCAT GGACTGTGCC CGCCCACAGC AGCACTCTCC CCAGCATGCC
    CATTCCGATG GGGCGGCTTC ACCATTCACC CCAGAATTCC TGGTCCAGCA GCGCTGGGGA
    GCTATGGAGG ATTCCTGTTT TGAGATCCAG AGCCCCCCCT CTTGTGCAGA CTCACAAAGC
    CAGATCATGG AGTACATTCG TAAGATCGAG GCTGACCTAG AACACCTAAA GAAGGTGGAA
    GAGAGTTACA GCATTCTTTG CCAAAGGCTG GCTGGATCAG CCCTCACAGA CAAGCACTCA
    GATAAGAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05840
    Clone ID Related Accession (Same CDS sequence) XM_005619663.1 , XM_005619663.2
    Accession Version XM_005619663.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726057.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGAGT
    ATGAAGAAAG ATCGAGAAGG CGAAGAAAAA GGAAAGCGCA GAGGATTCCC CAGCATCCTG
    GGACCCCCAA GGAGACCAAG CCGTCACGAC AACAGTGCAA TTGGCAGAGC CATGGAACTA
    CAGAAGCAGC GCCACCCGAA GCACTTATCC ACACCTTCTT CTGTGAGTCC TGAGCCTCAG
    GACCCTGCCA AGTTGCGCCA GAGTGCATTA GCAGGCGAAG GAACAGAGGT CGGATACCTG
    CCAGCCAATC CCATGTCCTC TGTGGCTGCT GGGGCCACTC TTTCCCAGGA AGGAGGGAAG
    GAGAATGATA CAGGATCAAA GCAAGTTGGA GAAACACCAG CATCTGGAGA CTGCATAGAT
    AATACCCCTC GGACTCCCAA TACCATCTTT GATTTCCCAC CTCCTCCCTT AGACCAAGTG
    CAGGAGGAGG AATGGGAAGT GGAAAGGGTG ACTGAGCATG GGACACCAAA ACCCTTTCGA
    AAGTTTGACA GCATAGCTTT TGGAGAAAGT CAAAGTGAGG ATGAACAGTT TGAAAATGAC
    TTAGAGACAG ATCCACCCAA CTGGCAACAG CTCGTTAGTC GAGAAGTGTT ACTGGGCCTA
    AAGCCTTCTG AGATCAAAAG ACAGGAAGTA ATTAATGAAT TGTTCTACAC TGAAAGAGCT
    CATGTCCGAA CACTAAAGGT TCTTGATCAA GTATTCTATC AGCGAGTGTC CAGGGAAGGA
    ATTCTGTCCC CTTCAGAACT ACGGAAGATT TTTTCAAACT TGGAAGATAT TCTTCAACTT
    CACATTGGAT TGAATGAGCA AATGAAGGCT GTTCGAAAGA GGAATGAGAC CTCAGTTATT
    GATCAGATTG GGGAAGATTT GCTGACCTGG TTCAGTGGAC CAGGGGAGGA CAAGCTGAAG
    CACGCCGCTG CTACCTTTTG CAGTAACCAA CCTTTTGCCC TGGAAATGAT CAAGTCTCGT
    CAGAAAAAGG ATTCTCGATT CCAGACCTTT GTGCAAGATG CTGAGAGTAA TCCTCTGTGT
    CGTCGTCTGC AGCTGAAGGA TATCATTCCT ACCCAGATGC AGAGGCTTAC CAAGTACCCT
    CTTCTGTTGG ATAATATTGC CAAATACACA GAATGGCCAA TGGAAAGGGA GAAGGTGAAG
    AAAGCCGCAG ATCACTGTCG TCAGATCTTA AATTACGTAA ATCAGGCTGT CAAGGAGGCA
    GAAAACAAAC AGCGACTAGA AGACTACCAG CGTCGCCTGG ATACCTCCAA TCTGAAGTTG
    TCAGAGTACC CAAATGTGGA AGAGCTCAGG AATTTGGATT TAACGAAAAG GAAGATGATT
    CATGAAGGGC CATTGGTCTG GAAGGTGAAT CGAGATAAAA CTATTGATCT ATACACATTG
    CTGCTGGAAG ACATTCTTGT ATTGTTACAA AAGCAGGATG ATAGACTGGT GTTAAGATGT
    CATAGTAAGA TTCTGGCATC CACGGCTGAC AGCAAACACA CATTTAGCCC TGTCATTAAG
    TTGAATACAG TGTTGGTTCG ACAAGTGGCA ACAGATAACA AAGCTTTATT TGTCATCTCC
    ATGTCAGACA ATGGAGCCCA GATTTATGAA CTGGTGGCAC AGACTGTTTC TGAAAAGACT
    GTCTGGCAAG ACCTAATCTG TCGGATGGCT GCATCAGTGA AGGAACAGTC CGCAAAGCCA
    ATCCCATTGC CACAGTCACC ACCTTGCGAA GGAGACAACG ATGAAGAAGC AGCTCCAAAG
    TTAAAAGTGG AACATCATAG CATTTCAGTC CCTGGTTTGC AGAGTCCAGA CAGAGATTTG
    GGATTAGAGT CTCCCTTAAT ATCATCAAAA CCTCAGTCTC ATTCACTGGG TACCTCTGGG
    AAATCCGAGG TAGATGATCT GTTTGTGGCC GAGAGGCAGT TTGCTAAGGA GCAGCATACA
    GATGGGACTC TGCAGGAAGC TGGAGGAAGT TATCAGATCA CAATCCCAGA TCCACACTTG
    CCTGCCTCCG AAGAACGGTG GGCACTGGAT GCACTAAGGA ATTTGGGCTT GCTGAAGCAG
    TTGCTCGTCC AACAGCTGGG TTTGACCGAG AAGAGTGCTC AGGAAGACTG GCCACATTTC
    CCGAGATACA GGACGGCGTC TTCAGGGGTG CAGACAGACA GTGGAACCCA GAACTCTGAA
    AACAGTAAGG CCTATCCTCC TGGTGAAGGA CAGATGCCCT TTAGAACTGG AACTGGTGAC
    ATTTCAACTT GTTACAGTCC ACGGACTTCC ACTGAATCTT CTGCTCCACG GGATTCAGTG
    GTCCTGGCAT TCCAAGAGAG CCAGGCAAGT AACATTTTAG TAATGGACCA CATGATTATG
    ACCCCAGAGA TGCCTCCTGC AGAGCCAGAA GGGAGTCTTG ATGAGAGTGG AGAGCACTTT
    TTTGATGCCC GTGAAGCACA TAGTGATGAT AATCCATCAG AAGGTGATGG AGCAGTTAAG
    AAGGAAGAGG ATGTTAATTT GCGCATCTCA GGAAACTATT TGATTCTTGA TGGCTATGAA
    GCGGTGCAGG AGAGCTCCAC GGACGAGGAG GTTGCTTCCT CGCTTCCCCC GCCGCCCACG
    GCAGGCATCC CCGCCATGGA CTGTGCCCGC CCACAGCAGC ACTCTCCCCA GCATGCCCAT
    TCCGATGGGG CGGCTTCACC ATTCACCCCA GAATTCCTGG TCCAGCAGCG CTGGGGAGCT
    ATGGAGGATT CCTGTTTTGA GATCCAGAGC CCCCCCTCTT GTGCAGACTC ACAAAGCCAG
    ATCATGGAGT ACATTCGTAA GATCGAGGCT GACCTAGAAC ACCTAAAGAA GGTGGAAGAG
    AGTTACAGCA TTCTTTGCCA AAGGCTGGCT GGATCAGCCC TCACAGACAA GCACTCAGAT
    AAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619720.1
    CDS70..4698
    Translation

    Target ORF information:

    RefSeq Version XM_005619663.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 12 (ARHGEF12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619663.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGAGT
    ATGAAGAAAG ATCGAGAAGG CGAAGAAAAA GGAAAGCGCA GAGGATTCCC CAGCATCCTG
    GGACCCCCAA GGAGACCAAG CCGTCACGAC AACAGTGCAA TTGGCAGAGC CATGGAACTA
    CAGAAGCAGC GCCACCCGAA GCACTTATCC ACACCTTCTT CTGTGAGTCC TGAGCCTCAG
    GACCCTGCCA AGTTGCGCCA GAGTGCATTA GCAGGCGAAG GAACAGAGGT CGGATACCTG
    CCAGCCAATC CCATGTCCTC TGTGGCTGCT GGGGCCACTC TTTCCCAGGA AGGAGGGAAG
    GAGAATGATA CAGGATCAAA GCAAGTTGGA GAAACACCAG CATCTGGAGA CTGCATAGAT
    AATACCCCTC GGACTCCCAA TACCATCTTT GATTTCCCAC CTCCTCCCTT AGACCAAGTG
    CAGGAGGAGG AATGGGAAGT GGAAAGGGTG ACTGAGCATG GGACACCAAA ACCCTTTCGA
    AAGTTTGACA GCATAGCTTT TGGAGAAAGT CAAAGTGAGG ATGAACAGTT TGAAAATGAC
    TTAGAGACAG ATCCACCCAA CTGGCAACAG CTCGTTAGTC GAGAAGTGTT ACTGGGCCTA
    AAGCCTTCTG AGATCAAAAG ACAGGAAGTA ATTAATGAAT TGTTCTACAC TGAAAGAGCT
    CATGTCCGAA CACTAAAGGT TCTTGATCAA GTATTCTATC AGCGAGTGTC CAGGGAAGGA
    ATTCTGTCCC CTTCAGAACT ACGGAAGATT TTTTCAAACT TGGAAGATAT TCTTCAACTT
    CACATTGGAT TGAATGAGCA AATGAAGGCT GTTCGAAAGA GGAATGAGAC CTCAGTTATT
    GATCAGATTG GGGAAGATTT GCTGACCTGG TTCAGTGGAC CAGGGGAGGA CAAGCTGAAG
    CACGCCGCTG CTACCTTTTG CAGTAACCAA CCTTTTGCCC TGGAAATGAT CAAGTCTCGT
    CAGAAAAAGG ATTCTCGATT CCAGACCTTT GTGCAAGATG CTGAGAGTAA TCCTCTGTGT
    CGTCGTCTGC AGCTGAAGGA TATCATTCCT ACCCAGATGC AGAGGCTTAC CAAGTACCCT
    CTTCTGTTGG ATAATATTGC CAAATACACA GAATGGCCAA TGGAAAGGGA GAAGGTGAAG
    AAAGCCGCAG ATCACTGTCG TCAGATCTTA AATTACGTAA ATCAGGCTGT CAAGGAGGCA
    GAAAACAAAC AGCGACTAGA AGACTACCAG CGTCGCCTGG ATACCTCCAA TCTGAAGTTG
    TCAGAGTACC CAAATGTGGA AGAGCTCAGG AATTTGGATT TAACGAAAAG GAAGATGATT
    CATGAAGGGC CATTGGTCTG GAAGGTGAAT CGAGATAAAA CTATTGATCT ATACACATTG
    CTGCTGGAAG ACATTCTTGT ATTGTTACAA AAGCAGGATG ATAGACTGGT GTTAAGATGT
    CATAGTAAGA TTCTGGCATC CACGGCTGAC AGCAAACACA CATTTAGCCC TGTCATTAAG
    TTGAATACAG TGTTGGTTCG ACAAGTGGCA ACAGATAACA AAGCTTTATT TGTCATCTCC
    ATGTCAGACA ATGGAGCCCA GATTTATGAA CTGGTGGCAC AGACTGTTTC TGAAAAGACT
    GTCTGGCAAG ACCTAATCTG TCGGATGGCT GCATCAGTGA AGGAACAGTC CGCAAAGCCA
    ATCCCATTGC CACAGTCACC ACCTTGCGAA GGAGACAACG ATGAAGAAGC AGCTCCAAAG
    TTAAAAGTGG AACATCATAG CATTTCAGTC CCTGGTTTGC AGAGTCCAGA CAGAGATTTG
    GGATTAGAGT CTCCCTTAAT ATCATCAAAA CCTCAGTCTC ATTCACTGGG TACCTCTGGG
    AAATCCGAGG TAGATGATCT GTTTGTGGCC GAGAGGCAGT TTGCTAAGGA GCAGCATACA
    GATGGGACTC TGCAGGAAGC TGGAGGAAGT TATCAGATCA CAATCCCAGA TCCACACTTG
    CCTGCCTCCG AAGAACGGTG GGCACTGGAT GCACTAAGGA ATTTGGGCTT GCTGAAGCAG
    TTGCTCGTCC AACAGCTGGG TTTGACCGAG AAGAGTGCTC AGGAAGACTG GCCACATTTC
    CCGAGATACA GGACGGCGTC TTCAGGGGTG CAGACAGACA GTGGAACCCA GAACTCTGAA
    AACAGTAAGG CCTATCCTCC TGGTGAAGGA CAGATGCCCT TTAGAACTGG AACTGGTGAC
    ATTTCAACTT GTTACAGTCC ACGGACTTCC ACTGAATCTT CTGCTCCACG GGATTCAGTG
    GTCCTGGCAT TCCAAGAGAG CCAGGCAAGT AACATTTTAG TAATGGACCA CATGATTATG
    ACCCCAGAGA TGCCTCCTGC AGAGCCAGAA GGGAGTCTTG ATGAGAGTGG AGAGCACTTT
    TTTGATGCCC GTGAAGCACA TAGTGATGAT AATCCATCAG AAGGTGATGG AGCAGTTAAG
    AAGGAAGAGG ATGTTAATTT GCGCATCTCA GGAAACTATT TGATTCTTGA TGGCTATGAA
    GCGGTGCAGG AGAGCTCCAC GGACGAGGAG GTTGCTTCCT CGCTTCCCCC GCCGCCCACG
    GCAGGCATCC CCGCCATGGA CTGTGCCCGC CCACAGCAGC ACTCTCCCCA GCATGCCCAT
    TCCGATGGGG CGGCTTCACC ATTCACCCCA GAATTCCTGG TCCAGCAGCG CTGGGGAGCT
    ATGGAGGATT CCTGTTTTGA GATCCAGAGC CCCCCCTCTT GTGCAGACTC ACAAAGCCAG
    ATCATGGAGT ACATTCGTAA GATCGAGGCT GACCTAGAAC ACCTAAAGAA GGTGGAAGAG
    AGTTACAGCA TTCTTTGCCA AAGGCTGGCT GGATCAGCCC TCACAGACAA GCACTCAGAT
    AAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe05840
    Clone ID Related Accession (Same CDS sequence) XM_005619663.1 , XM_005619663.2
    Accession Version XM_005619663.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product rho guanine nucleotide exchange factor 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005619663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGAGT
    ATGAAGAAAG ATCGAGAAGG CGAAGAAAAA GGAAAGCGCA GAGGATTCCC CAGCATCCTG
    GGACCCCCAA GGAGACCAAG CCGTCACGAC AACAGTGCAA TTGGCAGAGC CATGGAACTA
    CAGAAGCAGC GCCACCCGAA GCACTTATCC ACACCTTCTT CTGTGAGTCC TGAGCCTCAG
    GACCCTGCCA AGTTGCGCCA GAGTGCATTA GCAGGCGAAG GAACAGAGGT CGGATACCTG
    CCAGCCAATC CCATGTCCTC TGTGGCTGCT GGGGCCACTC TTTCCCAGGA AGGAGGGAAG
    GAGAATGATA CAGGATCAAA GCAAGTTGGA GAAACACCAG CATCTGGAGA CTGCATAGAT
    AATACCCCTC GGACTCCCAA TACCATCTTT GATTTCCCAC CTCCTCCCTT AGACCAAGTG
    CAGGAGGAGG AATGGGAAGT GGAAAGGGTG ACTGAGCATG GGACACCAAA ACCCTTTCGA
    AAGTTTGACA GCATAGCTTT TGGAGAAAGT CAAAGTGAGG ATGAACAGTT TGAAAATGAC
    TTAGAGACAG ATCCACCCAA CTGGCAACAG CTCGTTAGTC GAGAAGTGTT ACTGGGCCTA
    AAGCCTTCTG AGATCAAAAG ACAGGAAGTA ATTAATGAAT TGTTCTACAC TGAAAGAGCT
    CATGTCCGAA CACTAAAGGT TCTTGATCAA GTATTCTATC AGCGAGTGTC CAGGGAAGGA
    ATTCTGTCCC CTTCAGAACT ACGGAAGATT TTTTCAAACT TGGAAGATAT TCTTCAACTT
    CACATTGGAT TGAATGAGCA AATGAAGGCT GTTCGAAAGA GGAATGAGAC CTCAGTTATT
    GATCAGATTG GGGAAGATTT GCTGACCTGG TTCAGTGGAC CAGGGGAGGA CAAGCTGAAG
    CACGCCGCTG CTACCTTTTG CAGTAACCAA CCTTTTGCCC TGGAAATGAT CAAGTCTCGT
    CAGAAAAAGG ATTCTCGATT CCAGACCTTT GTGCAAGATG CTGAGAGTAA TCCTCTGTGT
    CGTCGTCTGC AGCTGAAGGA TATCATTCCT ACCCAGATGC AGAGGCTTAC CAAGTACCCT
    CTTCTGTTGG ATAATATTGC CAAATACACA GAATGGCCAA TGGAAAGGGA GAAGGTGAAG
    AAAGCCGCAG ATCACTGTCG TCAGATCTTA AATTACGTAA ATCAGGCTGT CAAGGAGGCA
    GAAAACAAAC AGCGACTAGA AGACTACCAG CGTCGCCTGG ATACCTCCAA TCTGAAGTTG
    TCAGAGTACC CAAATGTGGA AGAGCTCAGG AATTTGGATT TAACGAAAAG GAAGATGATT
    CATGAAGGGC CATTGGTCTG GAAGGTGAAT CGAGATAAAA CTATTGATCT ATACACATTG
    CTGCTGGAAG ACATTCTTGT ATTGTTACAA AAGCAGGATG ATAGACTGGT GTTAAGATGT
    CATAGTAAGA TTCTGGCATC CACGGCTGAC AGCAAACACA CATTTAGCCC TGTCATTAAG
    TTGAATACAG TGTTGGTTCG ACAAGTGGCA ACAGATAACA AAGCTTTATT TGTCATCTCC
    ATGTCAGACA ATGGAGCCCA GATTTATGAA CTGGTGGCAC AGACTGTTTC TGAAAAGACT
    GTCTGGCAAG ACCTAATCTG TCGGATGGCT GCATCAGTGA AGGAACAGTC CGCAAAGCCA
    ATCCCATTGC CACAGTCACC ACCTTGCGAA GGAGACAACG ATGAAGAAGC AGCTCCAAAG
    TTAAAAGTGG AACATCATAG CATTTCAGTC CCTGGTTTGC AGAGTCCAGA CAGAGATTTG
    GGATTAGAGT CTCCCTTAAT ATCATCAAAA CCTCAGTCTC ATTCACTGGG TACCTCTGGG
    AAATCCGAGG TAGATGATCT GTTTGTGGCC GAGAGGCAGT TTGCTAAGGA GCAGCATACA
    GATGGGACTC TGCAGGAAGC TGGAGGAAGT TATCAGATCA CAATCCCAGA TCCACACTTG
    CCTGCCTCCG AAGAACGGTG GGCACTGGAT GCACTAAGGA ATTTGGGCTT GCTGAAGCAG
    TTGCTCGTCC AACAGCTGGG TTTGACCGAG AAGAGTGCTC AGGAAGACTG GCCACATTTC
    CCGAGATACA GGACGGCGTC TTCAGGGGTG CAGACAGACA GTGGAACCCA GAACTCTGAA
    AACAGTAAGG CCTATCCTCC TGGTGAAGGA CAGATGCCCT TTAGAACTGG AACTGGTGAC
    ATTTCAACTT GTTACAGTCC ACGGACTTCC ACTGAATCTT CTGCTCCACG GGATTCAGTG
    GTCCTGGCAT TCCAAGAGAG CCAGGCAAGT AACATTTTAG TAATGGACCA CATGATTATG
    ACCCCAGAGA TGCCTCCTGC AGAGCCAGAA GGGAGTCTTG ATGAGAGTGG AGAGCACTTT
    TTTGATGCCC GTGAAGCACA TAGTGATGAT AATCCATCAG AAGGTGATGG AGCAGTTAAG
    AAGGAAGAGG ATGTTAATTT GCGCATCTCA GGAAACTATT TGATTCTTGA TGGCTATGAA
    GCGGTGCAGG AGAGCTCCAC GGACGAGGAG GTTGCTTCCT CGCTTCCCCC GCCGCCCACG
    GCAGGCATCC CCGCCATGGA CTGTGCCCGC CCACAGCAGC ACTCTCCCCA GCATGCCCAT
    TCCGATGGGG CGGCTTCACC ATTCACCCCA GAATTCCTGG TCCAGCAGCG CTGGGGAGCT
    ATGGAGGATT CCTGTTTTGA GATCCAGAGC CCCCCCTCTT GTGCAGACTC ACAAAGCCAG
    ATCATGGAGT ACATTCGTAA GATCGAGGCT GACCTAGAAC ACCTAAAGAA GGTGGAAGAG
    AGTTACAGCA TTCTTTGCCA AAGGCTGGCT GGATCAGCCC TCACAGACAA GCACTCAGAT
    AAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005619720.1
    CDS76..4704
    Translation

    Target ORF information:

    RefSeq Version XM_005619663.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris Rho guanine nucleotide exchange factor 12 (ARHGEF12), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005619663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGCGGCA CACAGCCCAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
    GGGAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTGCA
    TCACATGATT TTGATCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG
    AGTAGATCCG AGATATATGG TCTGGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT
    GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTCTTCG TACAGTCTGT CAAAGAAGAT
    GGAGCAGCCA TGCGGGCTGG GGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT
    CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA
    GCTCTCACTG TTCAGGGACG CCCACCTGGG TCGCCCCAGA TTCCACTCGC TGATTCTGAA
    GTAGAGCCAT CAGTCATTGG ACATATGTCT CCCATCATGA CATCCCCTCA TTCACCTGGA
    GCATCTGGGA ACATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAGGA AAACAATGTG
    GTTCATAACC AGAAAGTAGA AATTCTGAGA AAGATGTTAC AGAAAGAACA GGAACGGCTA
    CAGTTATTGC AGGAAGATTA CAACCGAACA CCTGCCCAAA GATTGCTAAA AGAGATCCAA
    GAGGCCAAGA AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT
    CAGGATGGAG CTGTAGTTAC CTCCTCCAAG CCTGTAGGTG AGCCGCTGAC AGCCAGTGAG
    GTAGAAGCAG ATCCTGGTGA GGGAATAGGC AGACTTGACT ACTGCACTGG AGATGCTTCT
    CGGCCCGGCA GTGACAGCGC TGACAGTCCC AAGAGTGCCC TGAAAGAGAG GATTTGTCTA
    GAGGAAAACC CAGAGAAAAG TGAAGTGATT CAGGACACTG ATACCCAGTC TCTCTTTGGG
    AGCCCCTCTC CCCGTATAGC AGCTCATATT ATTGGAGCAG AGGATGATGA TTTCGGTACT
    GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTCCAGA GCATTGAACT GCTGAAATCT
    CGCCCTGCTC ACCTGGCTGT TTTCTTACAC CATGTAGTCT CACAGTTTGA CCCCGCCACA
    CTGCTTTGTT ATCTGTATTC AGACCTGTAT AAGCAGACCA ATTCCAAGGA GACTCGTCGC
    GTCTTCCTTG AGTTTCATCA GTTCTTCCTG GATCGATCTG CACACCTGAA AGTTTCTGTG
    CCTGATGAAA TATCAGTAGA TCTAGAAAAA AGGAGACCTG AGCTTATACC TGAGGATCTA
    CACCGCCACT ATATCCAAAC AATGCAGGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG
    GAGGATTTTC GGCAGAAGCG TAGCATGGGA CTGACCTTGG CTGAAAGCGA GCTGACTAAA
    CTCGACGCAG AGCGAGACAA GGACCGGGTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA
    CAGATTGTTG CCAAAATCGA AGAAGTGTTG ATGACTGCTC AGCCTGTGGA AGAAGATAAG
    AGTTCAACAA TGCAGTATGT TATCCTAATG TACATGAAGC ATTTGGGGGT AAAAGTGAAA
    GAACCTCGAA ATTTGGAGCA CAAGCGGGGT CGGATTGGAT TCCTTCCAAA AATCAAGAGT
    ATGAAGAAAG ATCGAGAAGG CGAAGAAAAA GGAAAGCGCA GAGGATTCCC CAGCATCCTG
    GGACCCCCAA GGAGACCAAG CCGTCACGAC AACAGTGCAA TTGGCAGAGC CATGGAACTA
    CAGAAGCAGC GCCACCCGAA GCACTTATCC ACACCTTCTT CTGTGAGTCC TGAGCCTCAG
    GACCCTGCCA AGTTGCGCCA GAGTGCATTA GCAGGCGAAG GAACAGAGGT CGGATACCTG
    CCAGCCAATC CCATGTCCTC TGTGGCTGCT GGGGCCACTC TTTCCCAGGA AGGAGGGAAG
    GAGAATGATA CAGGATCAAA GCAAGTTGGA GAAACACCAG CATCTGGAGA CTGCATAGAT
    AATACCCCTC GGACTCCCAA TACCATCTTT GATTTCCCAC CTCCTCCCTT AGACCAAGTG
    CAGGAGGAGG AATGGGAAGT GGAAAGGGTG ACTGAGCATG GGACACCAAA ACCCTTTCGA
    AAGTTTGACA GCATAGCTTT TGGAGAAAGT CAAAGTGAGG ATGAACAGTT TGAAAATGAC
    TTAGAGACAG ATCCACCCAA CTGGCAACAG CTCGTTAGTC GAGAAGTGTT ACTGGGCCTA
    AAGCCTTCTG AGATCAAAAG ACAGGAAGTA ATTAATGAAT TGTTCTACAC TGAAAGAGCT
    CATGTCCGAA CACTAAAGGT TCTTGATCAA GTATTCTATC AGCGAGTGTC CAGGGAAGGA
    ATTCTGTCCC CTTCAGAACT ACGGAAGATT TTTTCAAACT TGGAAGATAT TCTTCAACTT
    CACATTGGAT TGAATGAGCA AATGAAGGCT GTTCGAAAGA GGAATGAGAC CTCAGTTATT
    GATCAGATTG GGGAAGATTT GCTGACCTGG TTCAGTGGAC CAGGGGAGGA CAAGCTGAAG
    CACGCCGCTG CTACCTTTTG CAGTAACCAA CCTTTTGCCC TGGAAATGAT CAAGTCTCGT
    CAGAAAAAGG ATTCTCGATT CCAGACCTTT GTGCAAGATG CTGAGAGTAA TCCTCTGTGT
    CGTCGTCTGC AGCTGAAGGA TATCATTCCT ACCCAGATGC AGAGGCTTAC CAAGTACCCT
    CTTCTGTTGG ATAATATTGC CAAATACACA GAATGGCCAA TGGAAAGGGA GAAGGTGAAG
    AAAGCCGCAG ATCACTGTCG TCAGATCTTA AATTACGTAA ATCAGGCTGT CAAGGAGGCA
    GAAAACAAAC AGCGACTAGA AGACTACCAG CGTCGCCTGG ATACCTCCAA TCTGAAGTTG
    TCAGAGTACC CAAATGTGGA AGAGCTCAGG AATTTGGATT TAACGAAAAG GAAGATGATT
    CATGAAGGGC CATTGGTCTG GAAGGTGAAT CGAGATAAAA CTATTGATCT ATACACATTG
    CTGCTGGAAG ACATTCTTGT ATTGTTACAA AAGCAGGATG ATAGACTGGT GTTAAGATGT
    CATAGTAAGA TTCTGGCATC CACGGCTGAC AGCAAACACA CATTTAGCCC TGTCATTAAG
    TTGAATACAG TGTTGGTTCG ACAAGTGGCA ACAGATAACA AAGCTTTATT TGTCATCTCC
    ATGTCAGACA ATGGAGCCCA GATTTATGAA CTGGTGGCAC AGACTGTTTC TGAAAAGACT
    GTCTGGCAAG ACCTAATCTG TCGGATGGCT GCATCAGTGA AGGAACAGTC CGCAAAGCCA
    ATCCCATTGC CACAGTCACC ACCTTGCGAA GGAGACAACG ATGAAGAAGC AGCTCCAAAG
    TTAAAAGTGG AACATCATAG CATTTCAGTC CCTGGTTTGC AGAGTCCAGA CAGAGATTTG
    GGATTAGAGT CTCCCTTAAT ATCATCAAAA CCTCAGTCTC ATTCACTGGG TACCTCTGGG
    AAATCCGAGG TAGATGATCT GTTTGTGGCC GAGAGGCAGT TTGCTAAGGA GCAGCATACA
    GATGGGACTC TGCAGGAAGC TGGAGGAAGT TATCAGATCA CAATCCCAGA TCCACACTTG
    CCTGCCTCCG AAGAACGGTG GGCACTGGAT GCACTAAGGA ATTTGGGCTT GCTGAAGCAG
    TTGCTCGTCC AACAGCTGGG TTTGACCGAG AAGAGTGCTC AGGAAGACTG GCCACATTTC
    CCGAGATACA GGACGGCGTC TTCAGGGGTG CAGACAGACA GTGGAACCCA GAACTCTGAA
    AACAGTAAGG CCTATCCTCC TGGTGAAGGA CAGATGCCCT TTAGAACTGG AACTGGTGAC
    ATTTCAACTT GTTACAGTCC ACGGACTTCC ACTGAATCTT CTGCTCCACG GGATTCAGTG
    GTCCTGGCAT TCCAAGAGAG CCAGGCAAGT AACATTTTAG TAATGGACCA CATGATTATG
    ACCCCAGAGA TGCCTCCTGC AGAGCCAGAA GGGAGTCTTG ATGAGAGTGG AGAGCACTTT
    TTTGATGCCC GTGAAGCACA TAGTGATGAT AATCCATCAG AAGGTGATGG AGCAGTTAAG
    AAGGAAGAGG ATGTTAATTT GCGCATCTCA GGAAACTATT TGATTCTTGA TGGCTATGAA
    GCGGTGCAGG AGAGCTCCAC GGACGAGGAG GTTGCTTCCT CGCTTCCCCC GCCGCCCACG
    GCAGGCATCC CCGCCATGGA CTGTGCCCGC CCACAGCAGC ACTCTCCCCA GCATGCCCAT
    TCCGATGGGG CGGCTTCACC ATTCACCCCA GAATTCCTGG TCCAGCAGCG CTGGGGAGCT
    ATGGAGGATT CCTGTTTTGA GATCCAGAGC CCCCCCTCTT GTGCAGACTC ACAAAGCCAG
    ATCATGGAGT ACATTCGTAA GATCGAGGCT GACCTAGAAC ACCTAAAGAA GGTGGAAGAG
    AGTTACAGCA TTCTTTGCCA AAGGCTGGCT GGATCAGCCC TCACAGACAA GCACTCAGAT
    AAGAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.