ARHGEF12 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF12
Entrez Gene ID	503575
Full Name	Rho guanine nucleotide exchange factor 12
Synonyms	LARG
General protein information	Preferred Names Rho guanine nucleotide exchange factor 12 Names rho guanine nucleotide exchange factor 12 Rho guanine nucleotide exchange factor (GEF) 12 guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001123033.1	NP_001116505.1	rho guanine nucleotide exchange factor 12

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ARHGEF12	XP_005619719.1
	ARHGEF12	XP_002799913.1
Gallus gallus (chicken)	ARHGEF12	XP_417890.4
Danio rerio (zebrafish)	arhgef12	XP_005172066.1
Pan troglodytes (chimpanzee)	ARHGEF12	XP_001166830.1
Xenopus tropicalis (tropical clawed frog)	arhgef12	NP_001072496.1
Homo sapiens (human)	ARHGEF12	NP_056128.1
Bos taurus (cattle)	ARHGEF12	NP_001116505.1
Mus musculus (house mouse)	Arhgef12	NP_081420.2
Rattus norvegicus (Norway rat)	Arhgef12	NP_001013264.1
Danio rerio (zebrafish)	LOC562455	XP_003200092.2

Gene Ontology
Bibliography

Related articles in PubMed

Influence of simulated microgravity on the activation of the small GTPase Rho involved in cytoskeletal formation--molecular cloning and sequencing of bovine leukemia-associated guanine nucleotide exchange factor.
Higashibata A, Imamizo-Sato M, Seki M, Yamazaki T, Ishioka N
BMC biochemistry719(2006 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cDNA of LARG (leukemia-associated guanine nucleotide exchange factor) was identified, and its gene expression was observed to be downregulated under clinorotation, which subsequently resulted in the repression of RhoA activation
Title: Influence of simulated microgravity on the activation of the small GTPase Rho involved in cytoskeletal formation--molecular cloning and sequencing of bovine leukemia-associated guanine nucleotide exchange factor.

The following ARHGEF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa37427	NM_001123033.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 12 (ARHGEF12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF12 ( NM_001123033.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001116505.1 Bos taurus Rho guanine nucleotide exchange factor 12 (ARHGEF12), mRNA.

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CloneID	OBa37427
Clone ID Related Accession (Same CDS sequence)	NM_001123033.1
Accession Version	NM_001123033.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 12
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB188499.3. On Aug 18, 2008 this sequence version replaced XM_614812.3. ##Evidence-Data-START## CDS exon combination :: AB188499.3 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN05861908 [ECO:0000350] ##Evidence-Data-END##

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCCCA TTCACCTGGT 
GCATCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGG AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGCTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGACTTGACT GTGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC TGATAGTCCC AAGAGTGGCT TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAAAAAAG TGAAGCAATT CAGGACACTG ACACTCAGTC GCTTGTTGGG 
AGTCCCTCTA CCCGTGTGGC ACCTCATATT ATTGGAGCAG AAGACGATGA CTTTGGTAGT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTTCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACTTGGCTGT CTTCTTACAC CATGTAGTTT CCCAGTTTGA CCCTGCAACA 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAACAGACCA ATTCCAAGGA GACCCGTCGT 
GTCTTCCTTG AGTTCCATCA GTTCTTCCTG GATCGATCTG CTCACCTGAA GGTTTCTGTT 
CCTGAGGAAA TATCAGTAGA TCTAGAAAAA AGACGACCAG AGCTTATTCC TGAGGATCTA 
CATCGCCACT ATATCCAGAC TATGCAAGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAGCG CAGCATGGGG CTGACCCTGG CTGAAAGTGA ACTGACTAAA 
CTTGATGTAG AGCGAGACAA GGACCGGTTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTGTTG ATGACTGCCC AGGCTGTAGA AGAAGAAAAG 
AGTTCGACAA TGCAGTATGT TATTCTCATG TACATGAAAC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATAGAGA AGGCGAAGAA AAGGGGAAGC GAAGAGGATT CACCAGCATC 
TTGGGACCCC CTAGGAGACC AAGCCGCCAT GATAACAGCG CAATTGGCAG AGCCATGGAA 
CTGCAGAAGC AGCGCCACCC TAAGCACTTG TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGATTCTG CGAAGCTGCG CCAGAGTGGG TCAGCAAGTG AAGGAGCAGA CACTGGATAC 
CTGCCAGCCA ATTCCACGTC CTCCATGGTT GCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATATGGGATC GAAACAAGCT GGAGAAACGC CAGCATCTGG AGACTCCTTG 
GATGGCACTC CTCGTACTCC CAACACCATC TTTGATTTCC CACCTCCTCC ATTAGACCAG 
GTGCAGGAGG AAGAATGGGA AGTGGAGAGA GTGACTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAGAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CGGACCCACC CAACTGGCAA CAGCTTGTGA GTCGAGAAGT GTTATTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACATTGAA GGTTCTAGAT CAAGTGTTTT ATCAGCGAGT GTCCAGAGAA 
GGAATTTTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTAGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCTATTCGGA AGAGGAATGA GACCTCTGTT 
ATTGATCAGA TTGGGGAAGA TTTGCTGATC TGGTTCAGTG GACCCGGGGA GGAAAAGTTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCCTTTG CCCTGGAAAT GATCAAGTCT 
CGCCAGAAAA AGGATTCTCG ATTCCAGACT TTTGTGCAAG ACGCTGAGAG TAATCCGCTG 
TGTCGTCGTT TGCAGCTAAA GGATATCATT CCCACTCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTCC TGGACAATAT TGCCAAATAC ACAGAGTGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TGAACCAGGC TGTTAAGGAG 
GCAGAGAACA AGCAGCGCTT GGAAGATTAT CAGCGTCGCC TTGATACCTC TAATCTGAAG 
TTGTCAGAAT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCACGAAG GCCCATTGGT TTGGAAGGTG AATAGAGATA AAACAATTGA TCTGTATACA 
TTGCTGCTGG AAGATATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGG 
TGTCACAGTA AGATTCTGGC ATCTACAGCT GACAGCAAAC ACACATTTAG CCCCGTCATC 
AAGCTGAATA CGGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATC 
TCCATGTCTG ATAATGGAGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT ATGTCGGATG GCCGCATCCG TGAAGGAGCA GTCCACAAAG 
CCAATCCCAC TGCCCCAGCC GCCACCGTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAACATCA TGACATTTCA GTCACTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCTTT AATGTCATCA AAACCTCAGT CTCATTCGCT GGGTACCTCT 
GGGAAGTCGG AAGTAGATGA CCTCTTTGTG GCTGAGAGAC AGTTTGCAAA GGAGCAGCAT 
GCAGACGGGA CACTAAAGGA AGTCGGAGTG AATTATCAAG TCACAATCCC AGATCCACAC 
TTGCCTGGCT CAGAAGAACG GTGGGCCCTG GATGCACTAA GGAATTTGGG TTTGTTGAAG 
CAGCTGCTGG TACACCAGCT GGGCTTGACT GAGAAGAGCA CTCAGGAAGA CTGGCCACAT 
TTTCCAAGAT GCAGGACAGC CTCTCTGGGG GTGCAGGCAG ACAGTGGAAC CCAGAACCCT 
GAAAATATTA AGGCCTGTCA TCCTGTTGAA GCACAGATGC CCTTTAGAAC TGGAACTGGT 
GACATTTCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGGGATTCA 
GTAGTACTGG CATTCCAAGA GAGCCAGGCA AGTGACATTT TAATAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC CTCAGAGCCA GAAGGGGGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT 
AAAAAGGAAG AGAAGGATGT TAATTTGCGC ATCTCAGGAA CCTATCTGAT CCTTGATGGC 
TATGACCCGG TGCAGGAGAG CTCCACAGAC GACGAGGTTG CTTCCTCGTT CCCCCTTCAG 
CCCGGGGCAG GCATCCCCTC TCTGGACTCT GGACACCAGC AGCAGCCTTC TCCCCAGAAT 
GCTCATTCTG ATGGGGCTGT TTCACCGTTC AACCCAGAAT TACTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCT CTGCTTGTGC TGATTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAAATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116505.1
CDS	31..4665
Translation	MSGTQSTITDRFPLKKPIRHGSILNRESPTDKKQKVERSTSHDFDPTDSSSKKTKSSSEESRSEIYGLVQRCVIIQKDDNGFGLTVSGDNPVFVQSVKEDGAAMRAGVQTGDRIIKVNGTLVTHSNHLEVVKLIKSGSYVALTVQGRPPGSPQIPLADSEVEPSVTGHMSPIMTSPHSPGASGNMERITSPVLMGEENNVVHNQKVEILRKMLQKEQERLQLLQEDYNRTPAQRLLKEIQEAKEHIPQLQEQLSKATGSAQDGAVVTSSKPLADGLAVGEGEADPGDGLGRLDCGSGDASRPGSDSADSPKSGLKERIYLEENPEKSEAIQDTDTQSLVGSPSTRVAPHIIGAEDDDFGSEHEQINGQCSCFQSIELLKSRPAHLAVFLHHVVSQFDPATLLCYLYSDLYKQTNSKETRRVFLEFHQFFLDRSAHLKVSVPEEISVDLEKRRPELIPEDLHRHYIQTMQERVHPEVQRHLEDFRQKRSMGLTLAESELTKLDVERDKDRLTLEKERACAEQIVAKIEEVLMTAQAVEEEKSSTMQYVILMYMKHLGVKVKEPRNLEHKRGRIGFLPKIKQSMKKDREGEEKGKRRGFTSILGPPRRPSRHDNSAIGRAMELQKQRHPKHLSTPSSVSPEPQDSAKLRQSGSASEGADTGYLPANSTSSMVAGATLSQEGGKENDMGSKQAGETPASGDSLDGTPRTPNTIFDFPPPPLDQVQEEEWEVERVTEHGTPKPFRKFDSIAFGESQSEDEQFENDLETDPPNWQQLVSREVLLGLKPCEIKRQEVINELFYTERAHVRTLKVLDQVFYQRVSREGILSPSELRKIFSNLEDILQLHIGLNEQMKAIRKRNETSVIDQIGEDLLIWFSGPGEEKLKHAAATFCSNQPFALEMIKSRQKKDSRFQTFVQDAESNPLCRRLQLKDIIPTQMQRLTKYPLLLDNIAKYTEWPTEREKVKKAADHCRQILNYVNQAVKEAENKQRLEDYQRRLDTSNLKLSEYPNVEELRNLDLTKRKMIHEGPLVWKVNRDKTIDLYTLLLEDILVLLQKQDDRLVLRCHSKILASTADSKHTFSPVIKLNTVLVRQVATDNKALFVISMSDNGAQIYELVAQTVSEKTVWQDLICRMAASVKEQSTKPIPLPQPPPCEGDNDEEEPPKLKVEHHDISVTGLQSPDRDLGLESPLMSSKPQSHSLGTSGKSEVDDLFVAERQFAKEQHADGTLKEVGVNYQVTIPDPHLPGSEERWALDALRNLGLLKQLLVHQLGLTEKSTQEDWPHFPRCRTASLGVQADSGTQNPENIKACHPVEAQMPFRTGTGDISTCYSPRTSTESSAPRDSVVLAFQESQASDILIMDHMIMTPEMPPSEPEGGLDESGEHFFDAREAHSDDNPSEGDGAVKKEEKDVNLRISGTYLILDGYDPVQESSTDDEVASSFPLQPGAGIPSLDSGHQQQPSPQNAHSDGAVSPFNPELLVQQRWGAMEDSCFEIQSPSACADSQSQIMEYIRKIEADLEHLKKVEESYTILCQRLAGSALTDKHSDKS

Target ORF information:

RefSeq Version	NM_001123033.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 12 (ARHGEF12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123033.1

ORF Insert Sequence:

ATGAGTGGCA CACAGTCTAC TATCACCGAC AGGTTTCCCC TCAAAAAACC TATAAGGCAT 
GGAAGTATTT TGAACCGAGA GTCACCAACA GATAAGAAGC AGAAAGTTGA GCGCAGTACA 
TCACATGATT TTGACCCCAC AGATAGCTCC TCCAAGAAGA CAAAGTCTAG TTCAGAGGAG 
AGTAGATCCG AGATATATGG TCTTGTTCAG CGTTGTGTGA TCATCCAGAA GGATGACAAT 
GGATTTGGGC TGACGGTCAG TGGAGACAAT CCAGTGTTCG TACAGTCTGT CAAAGAAGAT 
GGAGCAGCCA TGCGGGCTGG AGTACAGACA GGTGATCGAA TCATCAAGGT GAATGGAACT 
CTGGTGACTC ATTCAAACCA TTTGGAAGTG GTGAAGCTAA TCAAATCTGG TTCCTATGTA 
GCTCTCACTG TTCAGGGACG CCCCCCTGGG TCGCCCCAGA TTCCACTTGC TGATTCTGAA 
GTAGAGCCAT CGGTCACTGG ACATATGTCT CCCATCATGA CATCCCCCCA TTCACCTGGT 
GCATCTGGGA ATATGGAGAG AATTACTAGT CCTGTGCTCA TGGGGGAAGA AAACAATGTG 
GTTCATAACC AGAAAGTAGA AATTCTGAGA AAAATGTTAC AGAAAGAACA GGAACGGCTT 
CAGCTATTGC AGGAAGACTA CAACCGAACA CCTGCTCAGA GATTGCTAAA AGAGATCCAA 
GAGGCCAAGG AACACATTCC TCAGCTGCAA GAGCAGTTAT CCAAAGCCAC AGGCTCTGCT 
CAGGATGGAG CTGTAGTTAC ATCCTCCAAG CCGCTAGCTG ACGGGCTAGC AGTGGGTGAA 
GGAGAAGCAG ATCCTGGTGA TGGGCTAGGC AGACTTGACT GTGGCAGCGG AGACGCTTCT 
CGGCCCGGTA GTGACAGTGC TGATAGTCCC AAGAGTGGCT TGAAAGAGAG GATTTATCTA 
GAGGAAAACC CAGAAAAAAG TGAAGCAATT CAGGACACTG ACACTCAGTC GCTTGTTGGG 
AGTCCCTCTA CCCGTGTGGC ACCTCATATT ATTGGAGCAG AAGACGATGA CTTTGGTAGT 
GAACATGAAC AGATCAATGG GCAGTGCAGC TGTTTTCAGA GCATTGAACT GCTAAAATCT 
CGCCCTGCTC ACTTGGCTGT CTTCTTACAC CATGTAGTTT CCCAGTTTGA CCCTGCAACA 
TTGCTCTGTT ATCTCTATTC AGACCTATAT AAACAGACCA ATTCCAAGGA GACCCGTCGT 
GTCTTCCTTG AGTTCCATCA GTTCTTCCTG GATCGATCTG CTCACCTGAA GGTTTCTGTT 
CCTGAGGAAA TATCAGTAGA TCTAGAAAAA AGACGACCAG AGCTTATTCC TGAGGATCTA 
CATCGCCACT ATATCCAGAC TATGCAAGAA AGAGTCCACC CAGAAGTTCA GAGGCACTTG 
GAAGATTTTC GGCAGAAGCG CAGCATGGGG CTGACCCTGG CTGAAAGTGA ACTGACTAAA 
CTTGATGTAG AGCGAGACAA GGACCGGTTA ACTTTGGAGA AGGAACGGGC ATGTGCAGAA 
CAGATTGTTG CCAAAATTGA AGAAGTGTTG ATGACTGCCC AGGCTGTAGA AGAAGAAAAG 
AGTTCGACAA TGCAGTATGT TATTCTCATG TACATGAAAC ATTTGGGAGT AAAAGTGAAA 
GAACCTCGAA ATTTGGAGCA CAAGCGAGGT CGGATTGGAT TCCTTCCAAA AATCAAGCAA 
AGTATGAAGA AAGATAGAGA AGGCGAAGAA AAGGGGAAGC GAAGAGGATT CACCAGCATC 
TTGGGACCCC CTAGGAGACC AAGCCGCCAT GATAACAGCG CAATTGGCAG AGCCATGGAA 
CTGCAGAAGC AGCGCCACCC TAAGCACTTG TCCACACCCT CATCTGTGAG TCCTGAACCT 
CAGGATTCTG CGAAGCTGCG CCAGAGTGGG TCAGCAAGTG AAGGAGCAGA CACTGGATAC 
CTGCCAGCCA ATTCCACGTC CTCCATGGTT GCAGGGGCCA CTCTTTCCCA GGAAGGAGGG 
AAAGAGAATG ATATGGGATC GAAACAAGCT GGAGAAACGC CAGCATCTGG AGACTCCTTG 
GATGGCACTC CTCGTACTCC CAACACCATC TTTGATTTCC CACCTCCTCC ATTAGACCAG 
GTGCAGGAGG AAGAATGGGA AGTGGAGAGA GTGACTGAAC ATGGGACACC AAAGCCCTTT 
CGAAAGTTTG ACAGCATAGC TTTTGGAGAA AGTCAGAGTG AGGATGAACA GTTTGAAAAT 
GACTTAGAGA CGGACCCACC CAACTGGCAA CAGCTTGTGA GTCGAGAAGT GTTATTGGGA 
CTAAAGCCTT GTGAGATCAA AAGACAGGAA GTAATTAATG AATTGTTCTA CACTGAAAGA 
GCTCATGTTC GAACATTGAA GGTTCTAGAT CAAGTGTTTT ATCAGCGAGT GTCCAGAGAA 
GGAATTTTGT CACCTTCAGA ACTACGGAAA ATTTTTTCAA ACTTAGAAGA TATTCTTCAA 
CTTCACATTG GATTGAATGA GCAAATGAAG GCTATTCGGA AGAGGAATGA GACCTCTGTT 
ATTGATCAGA TTGGGGAAGA TTTGCTGATC TGGTTCAGTG GACCCGGGGA GGAAAAGTTG 
AAACATGCTG CTGCTACCTT TTGCAGTAAC CAACCCTTTG CCCTGGAAAT GATCAAGTCT 
CGCCAGAAAA AGGATTCTCG ATTCCAGACT TTTGTGCAAG ACGCTGAGAG TAATCCGCTG 
TGTCGTCGTT TGCAGCTAAA GGATATCATT CCCACTCAAA TGCAGAGGCT TACTAAGTAC 
CCTCTTCTCC TGGACAATAT TGCCAAATAC ACAGAGTGGC CAACAGAAAG GGAGAAGGTG 
AAGAAAGCTG CAGATCACTG TCGTCAGATC TTAAATTATG TGAACCAGGC TGTTAAGGAG 
GCAGAGAACA AGCAGCGCTT GGAAGATTAT CAGCGTCGCC TTGATACCTC TAATCTGAAG 
TTGTCAGAAT ACCCAAATGT TGAAGAGCTC AGGAATTTGG ATTTAACAAA AAGGAAGATG 
ATTCACGAAG GCCCATTGGT TTGGAAGGTG AATAGAGATA AAACAATTGA TCTGTATACA 
TTGCTGCTGG AAGATATTCT TGTATTGTTA CAAAAGCAGG ATGATAGACT GGTGTTAAGG 
TGTCACAGTA AGATTCTGGC ATCTACAGCT GACAGCAAAC ACACATTTAG CCCCGTCATC 
AAGCTGAATA CGGTGTTGGT TCGACAAGTG GCAACAGATA ACAAAGCTTT ATTCGTCATC 
TCCATGTCTG ATAATGGAGC CCAAATTTAT GAACTGGTGG CACAGACAGT TTCTGAAAAG 
ACTGTCTGGC AGGACCTAAT ATGTCGGATG GCCGCATCCG TGAAGGAGCA GTCCACAAAG 
CCAATCCCAC TGCCCCAGCC GCCACCGTGC GAAGGAGACA ACGATGAAGA AGAACCTCCA 
AAATTAAAAG TGGAACATCA TGACATTTCA GTCACTGGTC TGCAGAGTCC AGACAGAGAT 
TTGGGATTAG AGTCTCCTTT AATGTCATCA AAACCTCAGT CTCATTCGCT GGGTACCTCT 
GGGAAGTCGG AAGTAGATGA CCTCTTTGTG GCTGAGAGAC AGTTTGCAAA GGAGCAGCAT 
GCAGACGGGA CACTAAAGGA AGTCGGAGTG AATTATCAAG TCACAATCCC AGATCCACAC 
TTGCCTGGCT CAGAAGAACG GTGGGCCCTG GATGCACTAA GGAATTTGGG TTTGTTGAAG 
CAGCTGCTGG TACACCAGCT GGGCTTGACT GAGAAGAGCA CTCAGGAAGA CTGGCCACAT 
TTTCCAAGAT GCAGGACAGC CTCTCTGGGG GTGCAGGCAG ACAGTGGAAC CCAGAACCCT 
GAAAATATTA AGGCCTGTCA TCCTGTTGAA GCACAGATGC CCTTTAGAAC TGGAACTGGT 
GACATTTCAA CTTGTTACAG TCCACGGACT TCAACTGAAT CTTCTGCTCC ACGGGATTCA 
GTAGTACTGG CATTCCAAGA GAGCCAGGCA AGTGACATTT TAATAATGGA CCACATGATT 
ATGACCCCAG AGATGCCTCC CTCAGAGCCA GAAGGGGGTC TTGATGAGAG TGGAGAGCAC 
TTTTTTGATG CCCGTGAAGC ACATAGTGAT GATAATCCAT CAGAAGGTGA TGGAGCAGTT 
AAAAAGGAAG AGAAGGATGT TAATTTGCGC ATCTCAGGAA CCTATCTGAT CCTTGATGGC 
TATGACCCGG TGCAGGAGAG CTCCACAGAC GACGAGGTTG CTTCCTCGTT CCCCCTTCAG 
CCCGGGGCAG GCATCCCCTC TCTGGACTCT GGACACCAGC AGCAGCCTTC TCCCCAGAAT 
GCTCATTCTG ATGGGGCTGT TTCACCGTTC AACCCAGAAT TACTGGTCCA GCAGCGCTGG 
GGAGCTATGG AGGATTCCTG TTTTGAGATC CAGAGTCCCT CTGCTTGTGC TGATTCACAA 
AGCCAGATCA TGGAGTACAT TCGTAAAATA GAGGCTGACC TTGAACACTT AAAGAAGGTG 
GAGGAAAGTT ACACCATTCT TTGCCAAAGG CTGGCTGGAT CAGCCCTCAC AGACAAGCAC 
TCAGATAAAA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Influence of simulated microgravity on the activation of the small GTPase Rho involved in cytoskeletal formation--molecular cloning and sequencing of bovine leukemia-associated guanine nucleotide exchange factor.
BMC biochemistry719(2006 Jun)
Higashibata A,Imamizo-Sato M,Seki M,Yamazaki T,Ishioka N

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ARHGEF12 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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