ACAN gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_006720419.1	XP_006720482.1	aggrecan core protein isoform X1
XM_017021985.1	XP_016877474.1	aggrecan core protein isoform X2
XM_011521313.1	XP_011519615.1	aggrecan core protein isoform X2
NM_013227.3	NP_037359.3	aggrecan core protein isoform 2 precursor
NM_001135.3	NP_001126.3	aggrecan core protein isoform 1 precursor
NM_001369268.1	NP_001356197.1	aggrecan core protein isoform 3 precursor
XM_017021987.1	XP_016877476.1	aggrecan core protein isoform X5
XM_017021986.1	XP_016877475.1	aggrecan core protein isoform X3
XM_011521314.1	XP_011519616.1	aggrecan core protein isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15353	NM_001135.3 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu22082	NM_013227.3 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu48152	NM_001369268.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu48152	XM_006720419.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu56217	XM_011521313.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu56218	XM_011521314.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu85295	XM_017021985.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu85296	XM_017021986.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu85297	XM_017021987.1 Latest version!	Homo sapiens aggrecan (ACAN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00

ACAN ( NM_001135.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001126.3 Homo sapiens aggrecan (ACAN), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15353
Clone ID Related Accession (Same CDS sequence)	NM_001135.3
Accession Version	NM_001135.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103982.10. On Aug 19, 2009 this sequence version replaced NM_001135.2. Transcript Variant: This variant (1) lacks three alternate in-frame exons in the 3' CDS, compared to variant 3. It encodes isoform 1, which lacks two internal segments, and is shorter, compared to isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2161674, SAMEA2467143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG 
GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC 
GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC 
GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC 
CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT 
GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG 
ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC 
ACGTGTAAAA AGGGCACAGC CACCACCTAC AAACGCAGAC TACAGAAGCG GAGCTCACGG 
CACCCTCGGA GGAGCCGCCC CAGCACAGCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001126.3
CDS	375..7670
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAADQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTATTYKRRLQKRSSRHPRRSRPSTAH

Target ORF information:

RefSeq Version	NM_001135.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135.3

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG 
GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC 
GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC 
GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC 
CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT 
GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG 
ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC 
ACGTGTAAAA AGGGCACAGC CACCACCTAC AAACGCAGAC TACAGAAGCG GAGCTCACGG 
CACCCTCGGA GGAGCCGCCC CAGCACAGCC CACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( NM_013227.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037359.3 Homo sapiens aggrecan (ACAN), transcript variant 2, mRNA.

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CloneID	OHu22082
Clone ID Related Accession (Same CDS sequence)	NM_013227.3
Accession Version	NM_013227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103982.10. This sequence is a reference standard in the RefSeqGene project. On Aug 19, 2009 this sequence version replaced NM_013227.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' CDS, compared to variant 3. It encodes isoform 2, which lacks an internal segment, and is shorter, compared to isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC 
CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037359.3
CDS	375..7967
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAAAPARSCAEEPCGAGTCKETEGHVICLCPPGYTGEHCNIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDPTTYKRRLQKRSSRHPRRSRPSTAH

Target ORF information:

RefSeq Version	NM_013227.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013227.3

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC 
CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( NM_001369268.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356197.1 Homo sapiens aggrecan (ACAN), transcript variant 3, mRNA.

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CloneID	OHu48152
Clone ID Related Accession (Same CDS sequence)	XM_006720419.1 , NM_001369268.1
Accession Version	NM_001369268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103982.10. On May 7, 2019 this sequence version replaced XM_006720419.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC 
CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC 
TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG 
GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG 
GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG 
GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG 
ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC 
CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC 
GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG 
GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG 
AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG 
CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC 
TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG 
AGGAGCCGCC CCAGCACAGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356197.1
CDS	382..8088
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAAAPARSCAEEPCGAGTCKETEGHVICLCPPGYTGEHCNIDIDECLSSPCLNGATCVDAIDSFTCLCLPSYEGDLCEIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDPTTYKRRLQKRSSRHPRRSRPSTAH

Target ORF information:

RefSeq Version	NM_001369268.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369268.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC 
CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC 
TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG 
GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG 
GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG 
GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG 
ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC 
CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC 
GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG 
GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG 
AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG 
CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC 
TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG 
AGGAGCCGCC CCAGCACAGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_006720419.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006720482.1 Homo sapiens aggrecan (ACAN), transcript variant X1, mRNA.

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CloneID	OHu48152
Clone ID Related Accession (Same CDS sequence)	XM_006720419.1 , NM_001369268.1
Accession Version	XM_006720419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC 
CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC 
TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG 
GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG 
GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG 
GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG 
ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC 
CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC 
GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG 
GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG 
AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG 
CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC 
TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG 
AGGAGCCGCC CCAGCACAGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006720482.1
CDS	376..8082
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAAAPARSCAEEPCGAGTCKETEGHVICLCPPGYTGEHCNIDIDECLSSPCLNGATCVDAIDSFTCLCLPSYEGDLCEIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDPTTYKRRLQKRSSRHPRRSRPSTAH

Target ORF information:

RefSeq Version	XM_006720419.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006720419.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC 
CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC 
TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG 
GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG 
GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG 
GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG 
ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC 
CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC 
GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG 
GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG 
AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG 
CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC 
TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG 
AGGAGCCGCC CCAGCACAGC CCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_011521313.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519615.1 Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.

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CloneID	OHu56217
Clone ID Related Accession (Same CDS sequence)	XM_011521313.1
Accession Version	XM_011521313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7593bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT 
GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC 
ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC 
CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519615.1
CDS	376..7968
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAADIDECLSSPCLNGATCVDAIDSFTCLCLPSYEGDLCEIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDPTTYKRRLQKRSSRHPRRSRPSTAH

Target ORF information:

RefSeq Version	XM_011521313.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521313.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT 
GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC 
ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC 
CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_011521314.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519616.1 Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.

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CloneID	OHu56218
Clone ID Related Accession (Same CDS sequence)	XM_011521314.1
Accession Version	XM_011521314.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7479bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG 
GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC 
GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC 
GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC 
CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT 
GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG 
ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC 
ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC 
TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG 
GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG 
CCTCAGATCA CCTGCACAGA CCCCACCACC TACAAACGCA GACTACAGAA GCGGAGCTCA 
CGGCACCCTC GGAGGAGCCG CCCCAGCACA GCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519616.1
CDS	376..7854
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAADQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDPTTYKRRLQKRSSRHPRRSRPSTAH

Target ORF information:

RefSeq Version	XM_011521314.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521314.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG 
GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC 
GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC 
GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC 
CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT 
GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG 
ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC 
ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC 
TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG 
GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG 
CCTCAGATCA CCTGCACAGA CCCCACCACC TACAAACGCA GACTACAGAA GCGGAGCTCA 
CGGCACCCTC GGAGGAGCCG CCCCAGCACA GCCCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_017021985.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877474.1 Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.

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CloneID	OHu85295
Clone ID Related Accession (Same CDS sequence)	XM_017021985.1
Accession Version	XM_017021985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC 
TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG 
AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877474.1
CDS	376..8037
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAAAPARSCAEEPCGAGTCKETEGHVICLCPPGYTGEHCNIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDREHHPGHLAEHRVRFCQHSLPPVPSPGDPTPSTGLSKGREVGGLLSHK

Target ORF information:

RefSeq Version	XM_017021985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021985.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC 
AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC 
ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC 
TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG 
AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_017021986.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877475.1 Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.

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CloneID	OHu85296
Clone ID Related Accession (Same CDS sequence)	XM_017021986.1
Accession Version	XM_017021986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT 
GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC 
ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC 
TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG 
AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877475.1
CDS	376..8037
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAADIDECLSSPCLNGATCVDAIDSFTCLCLPSYEGDLCEIDQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDREHHPGHLAEHRVRFCQHSLPPVPSPGDPTPSTGLSKGREVGGLLSHK

Target ORF information:

RefSeq Version	XM_017021986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021986.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT 
GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC 
ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT 
GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC 
TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC 
CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC 
GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC 
TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC 
TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT 
AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG 
CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT 
GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG 
ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC 
TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG 
AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACAN ( XM_017021987.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877476.1 Homo sapiens aggrecan (ACAN), transcript variant X5, mRNA.

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CloneID	OHu85297
Clone ID Related Accession (Same CDS sequence)	XM_017021987.1
Accession Version	XM_017021987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7548bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	aggrecan core protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG 
GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC 
GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC 
GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC 
CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT 
GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG 
ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC 
ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC 
TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG 
GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG 
CCTCAGATCA CCTGCACAGA CCGTGAGCAT CACCCCGGCC ATCTCGCTGA GCACAGGGTT 
AGATTCTGCC AGCACAGCCT TCCCCCCGTC CCCTCTCCTG GGGACCCTAC ACCGTCCACA 
GGGTTGAGCA AGGGAAGGGA GGTCGGGGGG CTGCTCAGTC ACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877476.1
CDS	376..7923
Translation	MTTLLWVFVTLRVITAAVTVETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFRGISAVPSPGEEEGGTPTSPSGVEEWIVTQVVPGVAAVPVEEETTAVPSGETTAILEFTTEPENQTEWEPAYTPVGTSPLPGILPTWPPTGAATEESTEGPSATEVPSASEEPSPSEVPFPSEEPSPSEEPFPSVRPFPSVELFPSEEPFPSKEPSPSEEPSASEEPYTPSPPVPSWTELPSSGEESGAPDVSGDFTGSGDVSGHLDFSGQLSGDRASGLPSGDLDSSGLTSTVGSGLPVESGLPSGDEERIEWPSTPTVGELPSGAEILEGSASGVGDLSGLPSGEVLETSASGVGDLSGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETTAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETAAPGVEDISGLPSGEVLETTAPGVEEISGLPSGEVLETTAPGVDEISGLPSGEVLETTAPGVEEISGLPSGEVLETSTSAVGDLSGLPSGGEVLEISVSGVEDISGLPSGEVVETSASGIEDVSELPSGEGLETSASGVEDLSRLPSGEEVLEISASGFGDLSGLPSGGEGLETSASEVGTDLSGLPSGREGLETSASGAEDLSGLPSGKEDLVGSASGDLDLGKLPSGTLGSGQAPETSGLPSGFSGEYSGVDLGSGPPSGLPDFSGLPSGFPTVSLVDSTLVEVVTASTASELEGRGTIGISGAGEISGLPSSELDISGRASGLPSGTELSGQASGSPDVSGEIPGLFGVSGQPSGFPDTSGETSGVTELSGLSSGQPGISGEASGVLYGTSQPFGITDLSGETSGVPDLSGQPSGLPGFSGATSGVPDLVSGTTSGSGESSGITFVDTSLVEVAPTTFKEEEGLGSVELSGLPSGEADLSGKSGMVDVSGQFSGTVDSSGFTSQTPEFSGLPSGIAEVSGESSRAEIGSSLPSGAYYGSGTPSSFPTVSLVDRTLVESVTQAPTAQEAGEGPSGILELSGAHSGAPDMSGEHSGFLDLSGLQSGLIEPSGEPPGTPYFSGDFASTTNVSGESSVAMGTSGEASGLPEVTLITSEFVEGVTEPTISQELGQRPPVTHTPQLFESSGKVSTAGDISGATPVLPGSGVEVSSVPESSSETSAYPEAGFGASAAPEASREDSGSPDLSETTSAFHEANLERSSGLGVSGSTLTFQEGEASAAPEVSGESTTTSDVGTEAPGLPSATPTASGDRTEISGDLSGHTSQLGVVISTSIPESEWTQQTQRPAETHLEIESSSLLYSGEETHTVETATSPTDASIPASPEWKRESESTAADQEVCEEGWNKYQGHCYRHFPDRETWVDAERRCREQQSHLSSIVTPEEQEFVNNNAQDYQWIGLNDRTIEGDFRWSDGHPMQFENWRPNQPDNFFAAGEDCVVMIWHEKGEWNDVPCNYHLPFTCKKGTVACGEPPVVEHARTFGQKKDRYEINSLVRYQCTEGFVQRHMPTIRCQPSGHWEEPQITCTDREHHPGHLAEHRVRFCQHSLPPVPSPGDPTPSTGLSKGREVGGLLSHK

Target ORF information:

RefSeq Version	XM_017021987.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aggrecan (ACAN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017021987.1

ORF Insert Sequence:

ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG 
GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG 
ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG 
GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT 
CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC 
CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG 
GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC 
TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC 
ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC 
GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC 
TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG 
ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT 
CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG 
GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC 
TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC 
AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC 
TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC 
TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG 
GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC 
GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT 
GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC 
AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC 
CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC 
CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC 
CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC 
TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC 
CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG 
CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG 
TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT 
CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC 
AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG 
CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG 
GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT 
CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG 
ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT 
GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA 
GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT 
TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG 
CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC 
CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA 
GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG 
CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA 
TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT 
GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC 
TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA 
GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA 
GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA 
GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC 
ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT 
GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC 
CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT 
GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA 
GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA 
GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG 
GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC 
ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC 
AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC 
GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG 
CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT 
CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT 
TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT 
GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA 
GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA 
GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT 
CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA 
GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG 
ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT 
ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT 
ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT 
GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC 
TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT 
GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT 
GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT 
TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT 
TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA 
GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT 
GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC 
CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG 
AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG 
GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT 
GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA 
GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT 
GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT 
GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA 
GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG 
AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC 
CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC 
GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA 
ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC 
ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA 
GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT 
TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC 
ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA 
TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT 
ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA 
GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG 
TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG 
ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT 
GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC 
AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC 
AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC 
CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG 
GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG 
GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA 
GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT 
GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC 
CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT 
TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG 
GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC 
GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC 
GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC 
CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT 
GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG 
ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC 
ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC 
TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG 
GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG 
CCTCAGATCA CCTGCACAGA CCGTGAGCAT CACCCCGGCC ATCTCGCTGA GCACAGGGTT 
AGATTCTGCC AGCACAGCCT TCCCCCCGTC CCCTCTCCTG GGGACCCTAC ACCGTCCACA 
GGGTTGAGCA AGGGAAGGGA GGTCGGGGGG CTGCTCAGTC ACAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ACAN
Entrez Gene ID	176
Full Name	aggrecan
Synonyms	AGC1,AGCAN,CSPG1,CSPGCP,MSK16,SEDK,SSOAOD
General protein information	Preferred Names aggrecan Names aggrecan core protein cartilage-specific proteoglycan core protein chondroitin sulfate proteoglycan core protein 1 large aggregating proteoglycan
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q26.1
Summary	This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	155760
Disorder Html:	?Spondyloepiphyseal dysplasia, Kimberley type, 608361 (3); ?Spondyloepimetaphyseal dysplasia, aggrecan type, 612813 (3); Short stature and advanced bone age, with or without early-onset osteoarthritis and/or osteochondritis dissecans, 165800 (3)

Danio rerio (zebrafish)	acana	XP_005166542.1
Danio rerio (zebrafish)	acanb	XP_688042.4
Homo sapiens (human)	ACAN	NP_001126.3
Xenopus tropicalis (tropical clawed frog)	acan	XP_002932297.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ACAN cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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