ACAN cDNA ORF clone, Homo sapiens(human)

The following ACAN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACAN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu15353 NM_001135.3
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu22082 NM_013227.3
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu48152 NM_001369268.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu48152 XM_006720419.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu56217 XM_011521313.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu56218 XM_011521314.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu85295 XM_017021985.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu85296 XM_017021986.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu85297 XM_017021987.1
Latest version!
Homo sapiens aggrecan (ACAN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu15353
    Clone ID Related Accession (Same CDS sequence) NM_001135.3
    Accession Version NM_001135.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7296bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103982.10. On Aug 19, 2009 this sequence version replaced NM_001135.2. Transcript Variant: This variant (1) lacks three alternate in-frame exons in the 3' CDS, compared to variant 3. It encodes isoform 1, which lacks two internal segments, and is shorter, compared to isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2161674, SAMEA2467143 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG
    GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC
    GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC
    GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC
    CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT
    GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG
    ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC
    ACGTGTAAAA AGGGCACAGC CACCACCTAC AAACGCAGAC TACAGAAGCG GAGCTCACGG
    CACCCTCGGA GGAGCCGCCC CAGCACAGCC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001126.3
    CDS375..7670
    Translation

    Target ORF information:

    RefSeq Version NM_001135.3
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001135.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG
    GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC
    GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC
    GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC
    CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT
    GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG
    ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC
    ACGTGTAAAA AGGGCACAGC CACCACCTAC AAACGCAGAC TACAGAAGCG GAGCTCACGG
    CACCCTCGGA GGAGCCGCCC CAGCACAGCC CACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22082
    Clone ID Related Accession (Same CDS sequence) NM_013227.3
    Accession Version NM_013227.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103982.10. This sequence is a reference standard in the RefSeqGene project. On Aug 19, 2009 this sequence version replaced NM_013227.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 3' CDS, compared to variant 3. It encodes isoform 2, which lacks an internal segment, and is shorter, compared to isoform 3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2161674 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC
    CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_037359.3
    CDS375..7967
    Translation

    Target ORF information:

    RefSeq Version NM_013227.3
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_013227.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC
    CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48152
    Clone ID Related Accession (Same CDS sequence) XM_006720419.1 , NM_001369268.1
    Accession Version NM_001369268.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC103982.10. On May 7, 2019 this sequence version replaced XM_006720419.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC
    CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC
    TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG
    GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG
    GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG
    GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG
    ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC
    CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC
    GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG
    GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG
    AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG
    CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC
    TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG
    AGGAGCCGCC CCAGCACAGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001356197.1
    CDS382..8088
    Translation

    Target ORF information:

    RefSeq Version NM_001369268.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001369268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC
    CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC
    TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG
    GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG
    GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG
    GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG
    ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC
    CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC
    GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG
    GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG
    AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG
    CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC
    TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG
    AGGAGCCGCC CCAGCACAGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48152
    Clone ID Related Accession (Same CDS sequence) XM_006720419.1 , NM_001369268.1
    Accession Version XM_006720419.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7707bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC
    CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC
    TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG
    GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG
    GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG
    GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG
    ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC
    CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC
    GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG
    GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG
    AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG
    CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC
    TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG
    AGGAGCCGCC CCAGCACAGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720482.1
    CDS376..8082
    Translation

    Target ORF information:

    RefSeq Version XM_006720419.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006720419.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACAT TGATGAGTGC
    CTCTCAAGCC CTTGTCTGAA TGGAGCCACC TGCGTGGATG CCATCGACTC TTTCACATGC
    TTATGCCTTC CCAGCTACGA AGGGGACCTG TGTGAGATTG ACCAGGAGGT ATGTGAGGAG
    GGCTGGAACA AGTACCAGGG CCACTGTTAC CGCCACTTCC CGGACCGCGA GACCTGGGTG
    GATGCTGAGC GCCGGTGTCG GGAGCAGCAG TCACACCTGA GCAGCATCGT CACCCCCGAG
    GAGCAGGAGT TTGTCAACAA CAATGCCCAA GACTACCAGT GGATCGGCCT GAACGACAGG
    ACCATCGAAG GGGACTTCCG CTGGTCAGAT GGACACCCCA TGCAATTTGA GAACTGGCGC
    CCCAACCAGC CTGACAACTT TTTTGCCGCT GGAGAGGACT GTGTGGTGAT GATCTGGCAC
    GAGAAGGGCG AGTGGAATGA TGTTCCCTGC AATTACCACC TCCCCTTCAC GTGTAAAAAG
    GGCACAGTGG CCTGCGGAGA GCCCCCTGTG GTGGAGCATG CCAGGACCTT CGGGCAGAAG
    AAGGACCGGT ATGAGATCAA TTCCCTGGTG CGGTACCAGT GCACAGAGGG GTTTGTCCAG
    CGCCACATGC CCACCATCCG GTGCCAGCCC AGCGGGCACT GGGAGGAGCC TCAGATCACC
    TGCACAGACC CCACCACCTA CAAACGCAGA CTACAGAAGC GGAGCTCACG GCACCCTCGG
    AGGAGCCGCC CCAGCACAGC CCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56217
    Clone ID Related Accession (Same CDS sequence) XM_011521313.1
    Accession Version XM_011521313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7593bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT
    GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC
    ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC
    CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519615.1
    CDS376..7968
    Translation

    Target ORF information:

    RefSeq Version XM_011521313.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT
    GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC
    ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCCCAC CACCTACAAA CGCAGACTAC AGAAGCGGAG CTCACGGCAC
    CCTCGGAGGA GCCGCCCCAG CACAGCCCAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu56218
    Clone ID Related Accession (Same CDS sequence) XM_011521314.1
    Accession Version XM_011521314.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7479bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG
    GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC
    GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC
    GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC
    CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT
    GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG
    ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC
    ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC
    TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG
    GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG
    CCTCAGATCA CCTGCACAGA CCCCACCACC TACAAACGCA GACTACAGAA GCGGAGCTCA
    CGGCACCCTC GGAGGAGCCG CCCCAGCACA GCCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519616.1
    CDS376..7854
    Translation

    Target ORF information:

    RefSeq Version XM_011521314.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521314.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG
    GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC
    GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC
    GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC
    CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT
    GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG
    ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC
    ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC
    TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG
    GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG
    CCTCAGATCA CCTGCACAGA CCCCACCACC TACAAACGCA GACTACAGAA GCGGAGCTCA
    CGGCACCCTC GGAGGAGCCG CCCCAGCACA GCCCACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85295
    Clone ID Related Accession (Same CDS sequence) XM_017021985.1
    Accession Version XM_017021985.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
    3061
    3121
    3181
    3241
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    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
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    5401
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    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
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    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC
    TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG
    AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877474.1
    CDS376..8037
    Translation

    Target ORF information:

    RefSeq Version XM_017021985.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021985.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGCCCCCGCC
    AGGTCCTGTG CAGAGGAGCC CTGTGGAGCT GGGACCTGCA AGGAGACAGA GGGACACGTC
    ATATGCCTGT GCCCCCCTGG CTACACTGGC GAGCACTGTA ACATAGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC
    TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG
    AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85296
    Clone ID Related Accession (Same CDS sequence) XM_017021986.1
    Accession Version XM_017021986.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT
    GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC
    ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC
    TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG
    AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877475.1
    CDS376..8037
    Translation

    Target ORF information:

    RefSeq Version XM_017021986.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021986.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACATTGAT
    GAGTGCCTCT CAAGCCCTTG TCTGAATGGA GCCACCTGCG TGGATGCCAT CGACTCTTTC
    ACATGCTTAT GCCTTCCCAG CTACGAAGGG GACCTGTGTG AGATTGACCA GGAGGTATGT
    GAGGAGGGCT GGAACAAGTA CCAGGGCCAC TGTTACCGCC ACTTCCCGGA CCGCGAGACC
    TGGGTGGATG CTGAGCGCCG GTGTCGGGAG CAGCAGTCAC ACCTGAGCAG CATCGTCACC
    CCCGAGGAGC AGGAGTTTGT CAACAACAAT GCCCAAGACT ACCAGTGGAT CGGCCTGAAC
    GACAGGACCA TCGAAGGGGA CTTCCGCTGG TCAGATGGAC ACCCCATGCA ATTTGAGAAC
    TGGCGCCCCA ACCAGCCTGA CAACTTTTTT GCCGCTGGAG AGGACTGTGT GGTGATGATC
    TGGCACGAGA AGGGCGAGTG GAATGATGTT CCCTGCAATT ACCACCTCCC CTTCACGTGT
    AAAAAGGGCA CAGTGGCCTG CGGAGAGCCC CCTGTGGTGG AGCATGCCAG GACCTTCGGG
    CAGAAGAAGG ACCGGTATGA GATCAATTCC CTGGTGCGGT ACCAGTGCAC AGAGGGGTTT
    GTCCAGCGCC ACATGCCCAC CATCCGGTGC CAGCCCAGCG GGCACTGGGA GGAGCCTCAG
    ATCACCTGCA CAGACCGTGA GCATCACCCC GGCCATCTCG CTGAGCACAG GGTTAGATTC
    TGCCAGCACA GCCTTCCCCC CGTCCCCTCT CCTGGGGACC CTACACCGTC CACAGGGTTG
    AGCAAGGGAA GGGAGGTCGG GGGGCTGCTC AGTCACAAAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85297
    Clone ID Related Accession (Same CDS sequence) XM_017021987.1
    Accession Version XM_017021987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product aggrecan core protein isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG
    GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC
    GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC
    GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC
    CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT
    GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG
    ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC
    ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC
    TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG
    GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG
    CCTCAGATCA CCTGCACAGA CCGTGAGCAT CACCCCGGCC ATCTCGCTGA GCACAGGGTT
    AGATTCTGCC AGCACAGCCT TCCCCCCGTC CCCTCTCCTG GGGACCCTAC ACCGTCCACA
    GGGTTGAGCA AGGGAAGGGA GGTCGGGGGG CTGCTCAGTC ACAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016877476.1
    CDS376..7923
    Translation

    Target ORF information:

    RefSeq Version XM_017021987.1
    Organism Homo sapiens(human)
    Definition Homo sapiens aggrecan (ACAN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017021987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    ATGACCACTT TACTCTGGGT TTTCGTGACT CTGAGGGTCA TCACTGCAGC TGTCACTGTA 
    GAAACTTCAG ACCATGACAA CTCGCTGAGT GTCAGCATCC CCCAACCGTC CCCGCTGAGG
    GTCCTCCTGG GGACCTCCCT CACCATCCCC TGCTATTTCA TCGACCCCAT GCACCCTGTG
    ACCACCGCCC CTTCTACCGC CCCACTGGCC CCAAGAATCA AGTGGAGCCG TGTGTCCAAG
    GAGAAGGAGG TAGTGCTGCT GGTGGCCACT GAAGGGCGCG TGCGGGTCAA CAGTGCCTAT
    CAGGACAAGG TCTCACTGCC CAACTACCCG GCCATCCCCA GTGACGCCAC CTTGGAAGTC
    CAGAGCCTGC GCTCCAATGA CTCTGGGGTC TACCGCTGCG AGGTGATGCA TGGCATCGAG
    GACAGCGAGG CCACCCTGGA AGTCGTGGTG AAAGGCATCG TGTTCCATTA CAGAGCCATC
    TCTACACGCT ACACCCTCGA CTTTGACAGG GCGCAGCGGG CCTGCCTGCA GAACAGTGCC
    ATCATTGCCA CGCCTGAGCA GCTGCAGGCC GCCTACGAAG ACGGCTTCCA CCAGTGTGAC
    GCCGGCTGGC TGGCTGACCA GACTGTCAGA TACCCCATCC ACACTCCCCG GGAAGGCTGC
    TATGGAGACA AGGATGAGTT TCCTGGTGTG AGGACGTATG GCATCCGAGA CACCAACGAG
    ACCTATGATG TGTACTGCTT CGCCGAGGAG ATGGAGGGTG AGGTCTTTTA TGCAACATCT
    CCAGAGAAGT TCACCTTCCA GGAAGCAGCC AATGAGTGCC GGCGGCTGGG TGCCCGGCTG
    GCCACCACGG GCCAGCTCTA CCTGGCCTGG CAGGCTGGCA TGGACATGTG CAGCGCCGGC
    TGGCTGGCCG ACCGCAGCGT GCGCTACCCC ATCTCCAAGG CCCGGCCCAA CTGCGGTGGC
    AACCTCCTGG GCGTGAGGAC CGTCTACGTG CATGCCAACC AGACGGGCTA CCCCGACCCC
    TCATCCCGCT ACGACGCCAT CTGCTACACA GGTGAAGACT TTGTGGACAT CCCAGAAAAC
    TTCTTTGGAG TGGGGGGTGA GGAGGACATC ACCGTCCAGA CAGTGACCTG GCCTGACATG
    GAGCTGCCAC TGCCTCGAAA CATCACTGAG GGTGAAGCCC GAGGCAGCGT GATCCTTACC
    GTAAAGCCCA TCTTCGAGGT CTCCCCCAGT CCCCTGGAAC CCGAGGAGCC CTTCACGTTT
    GCCCCTGAAA TAGGGGCCAC TGCCTTCGCT GAGGTTGAGA ATGAGACTGG AGAGGCCACC
    AGGCCCTGGG GCTTTCCCAC ACCTGGCCTG GGCCCTGCCA CGGCATTCAC CAGTGAGGAC
    CTCGTCGTGC AGGTGACCGC TGTCCCTGGG CAGCCGCATT TGCCAGGGGG GGTCGTCTTC
    CACTACCGCC CGGGACCCAC CCGCTACTCG CTGACCTTTG AGGAGGCACA GCAGGCCTGC
    CTGCGCACGG GGGCGGTCAT TGCCTCGCCG GAGCAGCTCC AGGCCGCCTA CGAAGCAGGC
    TATGAGCAGT GTGACGCCGG CTGGCTGCGG GACCAGACCG TCAGATACCC CATTGTGAGC
    CCCCGGACCC CATGCGTGGG TGACAAGGAC AGCAGCCCAG GGGTCAGGAC CTATGGCGTG
    CGCCCATCAA CAGAGACCTA CGATGTCTAC TGCTTTGTAG ACAGACTTGA GGGGGAGGTG
    TTCTTCGCCA CACGCCTTGA GCAGTTCACC TTCCAGGAAG CACTGGAGTT CTGTGAATCT
    CACAATGCTA CGCTGGCCAC CACGGGCCAG CTCTACGCCG CCTGGAGCCG CGGCCTGGAC
    AAGTGCTATG CCGGCTGGCT GGCCGACGGC AGCCTCCGCT ACCCCATCGT CACCCCAAGG
    CCTGCCTGCG GTGGGGACAA GCCAGGCGTG AGAACGGTCT ACCTCTACCC TAACCAGACG
    GGCCTCCCAG ACCCACTGTC CCGGCACCAT GCCTTCTGCT TCCGAGGCAT TTCAGCGGTT
    CCTTCTCCAG GAGAAGAAGA GGGTGGCACA CCCACATCAC CCTCTGGTGT GGAGGAGTGG
    ATCGTGACCC AAGTGGTTCC TGGTGTGGCT GCTGTCCCCG TAGAAGAGGA GACAACTGCT
    GTACCCTCAG GGGAGACTAC TGCCATCCTA GAGTTCACCA CCGAGCCAGA AAACCAGACA
    GAATGGGAAC CAGCCTATAC CCCAGTGGGC ACATCCCCGC TGCCAGGGAT CCTTCCTACT
    TGGCCTCCCA CTGGCGCAGC AACAGAGGAA AGTACAGAAG GCCCTTCTGC AACTGAAGTG
    CCCTCTGCCT CAGAGGAACC ATCCCCCTCA GAGGTGCCAT TCCCCTCAGA GGAGCCATCC
    CCCTCAGAGG AACCATTCCC CTCAGTGAGG CCATTCCCCT CAGTGGAGCT GTTCCCCTCA
    GAGGAGCCAT TCCCCTCCAA GGAGCCATCC CCCTCAGAGG AACCATCAGC CTCGGAAGAG
    CCGTATACAC CTTCACCCCC CGTGCCCAGC TGGACTGAGC TGCCCAGCTC TGGGGAGGAA
    TCTGGGGCCC CTGATGTCAG TGGTGACTTC ACAGGCAGTG GAGATGTTTC AGGACACCTT
    GACTTCAGTG GGCAGCTGTC AGGGGACAGG GCAAGTGGAC TGCCCTCTGG AGACCTGGAC
    TCCAGTGGTC TTACTTCCAC AGTGGGCTCA GGCCTGCCTG TGGAAAGTGG ACTACCCTCA
    GGGGATGAAG AGAGAATTGA GTGGCCCAGC ACTCCTACGG TTGGTGAACT GCCCTCTGGA
    GCTGAGATCC TAGAGGGCTC TGCCTCTGGA GTTGGGGATC TCAGTGGACT TCCTTCTGGA
    GAAGTTCTAG AGACCTCTGC CTCTGGAGTA GGAGACCTCA GTGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CCACTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACCA CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACCACTG CCCCTGGAGT AGAGGACATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACCACTGCCC CTGGAGTAGA GGACATCAGC GGGCTTCCTT CTGGAGAAGT TCTAGAGACC
    ACTGCCCCTG GAGTAGAGGA CATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACCACT
    GCCCCTGGAG TAGAGGACAT CAGCGGGCTT CCTTCTGGAG AAGTTCTAGA GACCGCTGCC
    CCTGGAGTAG AGGACATCAG CGGGCTTCCT TCTGGAGAAG TTCTAGAGAC CGCTGCCCCT
    GGAGTAGAGG ACATCAGCGG GCTTCCTTCT GGAGAAGTTC TAGAGACCGC TGCCCCTGGA
    GTAGAGGACA TCAGCGGGCT TCCTTCTGGA GAAGTTCTAG AGACCGCTGC CCCTGGAGTA
    GAGGACATCA GCGGGCTTCC TTCTGGAGAA GTTCTAGAGA CCGCTGCCCC TGGAGTAGAG
    GACATCAGCG GGCTTCCTTC TGGAGAAGTT CTAGAGACCG CTGCCCCTGG AGTAGAGGAC
    ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA GAGACCGCTG CCCCTGGAGT AGAGGACATC
    AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG ACTGCTGCCC CTGGAGTAGA GGACATCAGC
    GGGCTTCCTT CTGGAGAAGT TCTAGAGACT GCTGCCCCTG GAGTAGAGGA CATCAGCGGG
    CTTCCTTCTG GAGAAGTTCT AGAGACTGCT GCCCCTGGAG TAGAGGACAT CAGCGGGCTT
    CCTTCTGGAG AAGTTCTAGA GACTGCTGCC CCTGGAGTAG AGGACATCAG CGGGCTTCCT
    TCTGGAGAAG TTCTAGAGAC TGCTGCCCCT GGAGTAGAGG ACATCAGCGG GCTTCCTTCT
    GGAGAAGTTC TAGAGACTGC TGCCCCTGGA GTAGAGGACA TCAGCGGGCT TCCTTCTGGA
    GAAGTTCTAG AGACTGCTGC CCCTGGAGTA GAGGACATCA GCGGGCTTCC TTCTGGAGAA
    GTTCTAGAGA CTGCTGCCCC TGGAGTAGAG GACATCAGCG GGCTTCCTTC TGGAGAAGTT
    CTAGAGACTG CTGCCCCTGG AGTAGAGGAC ATCAGCGGGC TTCCTTCTGG AGAAGTTCTA
    GAGACTACTG CCCCTGGAGT AGAGGAGATC AGCGGGCTTC CTTCTGGAGA AGTTCTAGAG
    ACTACTGCCC CTGGAGTAGA TGAGATCAGT GGGCTTCCTT CTGGAGAAGT TCTAGAGACT
    ACTGCCCCTG GAGTAGAGGA GATCAGCGGG CTTCCTTCTG GAGAAGTTCT AGAGACTTCT
    ACCTCTGCGG TAGGGGACCT CAGTGGACTT CCTTCTGGAG GAGAAGTTCT AGAGATTTCT
    GTCTCTGGAG TAGAGGACAT CAGTGGGCTT CCTTCTGGAG AGGTTGTAGA GACTTCTGCC
    TCTGGAATAG AGGATGTCAG TGAACTTCCT TCAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GGAGTAGAGG ACCTCAGCAG GCTCCCTTCT GGAGAAGAAG TTCTAGAGAT TTCTGCCTCT
    GGATTTGGGG ACCTCAGTGG ACTTCCTTCT GGAGGAGAAG GTCTAGAGAC CTCTGCTTCT
    GAAGTAGGGA CTGACCTCAG TGGGCTTCCT TCTGGAAGGG AGGGTCTAGA GACTTCAGCT
    TCTGGAGCTG AGGACCTCAG TGGGTTGCCT TCTGGAAAAG AAGACTTGGT GGGGTCAGCT
    TCTGGAGACT TGGACTTGGG CAAACTGCCT TCTGGAACTC TAGGAAGTGG GCAAGCTCCA
    GAAACAAGTG GTCTTCCCTC TGGATTTAGT GGTGAGTATT CTGGGGTGGA CCTTGGAAGT
    GGCCCACCCT CTGGCCTGCC TGACTTTAGT GGACTTCCAT CTGGATTCCC AACTGTTTCC
    CTAGTGGATT CTACATTGGT GGAAGTGGTC ACAGCCTCCA CTGCAAGTGA ACTGGAAGGG
    AGGGGAACCA TTGGCATCAG TGGTGCAGGA GAAATATCTG GACTGCCCTC CAGTGAGCTG
    GACATTAGTG GGAGAGCTAG TGGACTCCCT TCAGGAACTG AACTCAGTGG CCAAGCATCT
    GGGTCTCCTG ATGTCAGTGG GGAAATACCT GGACTCTTTG GTGTCAGTGG ACAGCCATCA
    GGGTTTCCTG ACACTAGTGG GGAAACATCT GGAGTGACTG AGCTTAGCGG GCTGTCCTCT
    GGACAACCAG GTATTAGTGG AGAAGCATCT GGAGTTCTTT ATGGCACTAG TCAACCCTTT
    GGCATAACTG ATCTGAGTGG AGAAACATCT GGGGTCCCTG ATCTCAGTGG GCAGCCTTCA
    GGGTTACCAG GGTTCAGTGG GGCAACATCA GGAGTCCCTG ACCTGGTTTC TGGTACCACG
    AGTGGCAGCG GTGAATCTTC TGGGATTACA TTTGTGGACA CCAGTTTGGT TGAAGTGGCC
    CCTACTACAT TTAAAGAAGA AGAAGGCTTA GGGTCTGTGG AACTCAGTGG CCTCCCTTCC
    GGAGAGGCAG ATCTGTCAGG CAAATCTGGG ATGGTGGATG TCAGTGGACA GTTTTCTGGA
    ACAGTCGATT CCAGTGGGTT TACATCCCAG ACTCCGGAAT TCAGTGGCCT ACCAAGTGGC
    ATAGCTGAGG TCAGTGGAGA ATCCTCCAGA GCTGAGATTG GGAGCAGCCT GCCCTCGGGA
    GCATATTATG GCAGTGGAAC TCCATCTAGT TTCCCCACTG TCTCTCTTGT AGACAGAACT
    TTGGTGGAAT CTGTAACCCA GGCTCCAACA GCCCAAGAGG CAGGAGAAGG GCCTTCTGGC
    ATTTTAGAAC TCAGTGGTGC TCATTCTGGA GCACCAGACA TGTCTGGGGA GCATTCTGGA
    TTTCTGGACC TAAGTGGGCT GCAGTCCGGG CTGATAGAGC CCAGCGGAGA GCCACCAGGT
    ACTCCATATT TTAGTGGGGA TTTTGCCAGC ACCACCAATG TAAGTGGAGA ATCCTCTGTA
    GCCATGGGCA CCAGTGGAGA GGCCTCAGGA CTTCCAGAAG TTACTTTAAT CACTTCTGAG
    TTCGTGGAGG GTGTTACTGA ACCAACTATT TCTCAGGAAC TAGGCCAAAG GCCCCCTGTG
    ACACACACAC CCCAGCTTTT TGAGTCCAGT GGAAAAGTCT CCACAGCTGG GGACATTAGT
    GGAGCTACCC CAGTGCTCCC TGGGTCTGGA GTAGAAGTAT CATCAGTCCC AGAATCTAGC
    AGTGAGACGT CCGCCTATCC TGAAGCTGGG TTCGGGGCAT CTGCCGCCCC TGAGGCCAGC
    AGAGAAGATT CTGGGTCCCC TGATCTGAGT GAAACCACCT CTGCATTCCA CGAAGCTAAC
    CTTGAGAGAT CCTCTGGCCT AGGAGTGAGC GGCAGCACTT TGACATTTCA AGAAGGCGAG
    GCGTCCGCTG CCCCAGAAGT GAGTGGAGAA TCCACCACCA CCAGTGATGT GGGGACAGAG
    GCACCAGGCT TGCCTTCAGC CACTCCCACG GCTTCTGGAG ACAGGACTGA AATCAGCGGA
    GACCTGTCTG GTCACACCTC GCAGCTGGGC GTTGTCATCA GCACCAGCAT CCCAGAGTCT
    GAGTGGACCC AGCAGACCCA GCGCCCTGCA GAGACGCATC TAGAAATTGA GTCCTCAAGC
    CTCCTGTACT CAGGAGAAGA GACTCACACA GTCGAAACAG CCACCTCCCC AACAGATGCT
    TCCATCCCAG CTTCTCCGGA ATGGAAACGT GAATCAGAAT CAACTGCTGC AGACCAGGAG
    GTATGTGAGG AGGGCTGGAA CAAGTACCAG GGCCACTGTT ACCGCCACTT CCCGGACCGC
    GAGACCTGGG TGGATGCTGA GCGCCGGTGT CGGGAGCAGC AGTCACACCT GAGCAGCATC
    GTCACCCCCG AGGAGCAGGA GTTTGTCAAC AACAATGCCC AAGACTACCA GTGGATCGGC
    CTGAACGACA GGACCATCGA AGGGGACTTC CGCTGGTCAG ATGGACACCC CATGCAATTT
    GAGAACTGGC GCCCCAACCA GCCTGACAAC TTTTTTGCCG CTGGAGAGGA CTGTGTGGTG
    ATGATCTGGC ACGAGAAGGG CGAGTGGAAT GATGTTCCCT GCAATTACCA CCTCCCCTTC
    ACGTGTAAAA AGGGCACAGT GGCCTGCGGA GAGCCCCCTG TGGTGGAGCA TGCCAGGACC
    TTCGGGCAGA AGAAGGACCG GTATGAGATC AATTCCCTGG TGCGGTACCA GTGCACAGAG
    GGGTTTGTCC AGCGCCACAT GCCCACCATC CGGTGCCAGC CCAGCGGGCA CTGGGAGGAG
    CCTCAGATCA CCTGCACAGA CCGTGAGCAT CACCCCGGCC ATCTCGCTGA GCACAGGGTT
    AGATTCTGCC AGCACAGCCT TCCCCCCGTC CCCTCTCCTG GGGACCCTAC ACCGTCCACA
    GGGTTGAGCA AGGGAAGGGA GGTCGGGGGG CTGCTCAGTC ACAAATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.