acanb cDNA ORF clone, Danio rerio(zebrafish)

The following acanb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acanb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa141351 XM_021470542.1
Latest version!
Danio rerio aggrecan b (acanb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa146385 XM_021473853.1
Latest version!
Danio rerio aggrecan b (acanb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa29379 XM_682950.6
Latest version!
Danio rerio aggrecan b (acanb), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa141351
    Clone ID Related Accession (Same CDS sequence) XM_021470542.1
    Accession Version XM_021470542.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4131bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product aggrecan core protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTTGA TTTGGCTCCT GCTGTATATA TGCACCCATG GCATTTCTGG CATGATATTA 
    TTTGGAGACA TGGCTGATCA AGACAGCACC CTCAGTGTGA GCATCCCTGT GGAACAGCCA
    TTGCGTCCCC TACTTGGGGG GAGCATGGTT ATCCCCTGCT ACTTTCAGGA CATCACCCTC
    CAGGACCCTG GCACCCTGAC CTCCCCTACC AATGCCCCAT TGTCCCAGCG CATTAAATGG
    AGTCATATCC ACAAGGGCAA AATATCCCTT ATACTGGTTG CCACTGAGGG TCAGGTGCAG
    GTTGAGACAG AGTACTTGGA CAGGGTTCAT CTGGTAAACT ACCCCATGGT GCCAAGTGAT
    GCTACCATTG AAATCTCAGA ACTCCGTTCC CATGATTCTG GCACCTACCG ATGTGAGGTC
    ATGCAGGGGA TTGACGATAA CTATGATTCG GTGGAAATGC AAATACAAGG CATTGTATTT
    CACTACCGAG CAATTTCTAC CCGATACACA TTAACCTTTG AAATGGCAAA AGCAGCCTGC
    ATTCAGAACA GTGCTGTTAT CGCAACTCCA GCACAGTTGC AAGCTGCCTA TGATGATGGA
    TTCCATCAGT GTGATGCTGG CTGGCTTTCA GACCAAACCG TGAGATATCC TATCCATGAA
    CCAAGGGAGC CTTGTTATGG AGACAAAGAT AATTTCCCAG GTGTTCGAAC ATATGGAGTC
    CGAGACGTCA ATGAAACCTA TGATGTGTAC TGTTTTGCAG AGAAAATGTC AGGCAGAGTG
    TTCTACTCTA TGTCTGTGGA AAAGTTCACC TTTGCAGATG CTGAAGACCA ATGTGCAAAG
    TTAGGAGCCC AATTAGCTAC CACAGGACAG CTGTACCTAG CATGGAAGAC TGGCATGGAT
    GTGTGCAACG CTGGCTGGCT TGCAGACAGG AGTGTGCGGT ACCCTATAAA TATAGCTAGA
    CCCCAATGTG GTGGGGGGCT TTTGGGAGTG CGAACAGTTT ACCTGTTTCC AAATCAGACT
    GGTTATCCAT ACCCAGACTC CCGCTATGAT GCAATCTGTT ATGAAGATAA AGAAGATAAG
    GTCTTAATAA AGACCACGCC ATTTCCAGAA ATATACACTA CCACCGAGTC AGTGTTAACA
    GTGGCTACAG TTACATCAAG CTCTTCAGAT TATACAGAAC AAGTGACCAC TGAAGGAGAG
    GTTCGTGGCC AAATATTGAC AGATGAGCCT TTTAACACAA CAAGCACAGA AAGTCCCCTA
    TCTCCACCTC TCAACATAAC TGATGTTGAG GAAGACATCA TCAATGTGGC CACAGCTCCA
    CCTTCCCTGG AACATGCAAT TCCCAGTATC AATGTCACTG CTAAAATCAA GGGAGTGGTA
    TTCCACTATC GTGCTGAATC TGGGCGCTAT GCTTACACTT ATGAGGAAGC ACAAGATGCT
    TGTCAGAAAC TGGGAGCAGT CATTGCCACT CCTGAGCTCC TTCAGGCTGC TTATGAAGCT
    GGCCTTCATC AATGCAGTGC AGGTTGGCTA CAAGATCAAA CTGTCAGATA TCCAATTGTG
    CATCCTCGAA AGAAATGCTC TGGAGATAGT GAGGATGTCC CTGGTGTACG CTCTTACAAT
    GTTAGGCCTG CACATGAACA ATATGATGTG TACTGTTACA TGGATCAAAT AAAGGGAGAG
    ATCTTCCATG TGAGCTCACT AGCTGGCTTT ACCTACTATG AAGCTGTTGC CCATTGCAGA
    AAACTGAGTT CTACTCTTGC CTCAACTGGA GAACTCTATG CAGCGTGGAA TCAGGGTTTC
    CACAAGTGCA GCCCTGGATG GCTGGCTGAC CGCAGTGTCC GCTACCCTGT ACGTAGCCCA
    GACTTTGATT GTGGTGGAAA CAAAACTGGA GTCCAGACAA TTTATGCCCA ACCAAACCAA
    ACAGGCTCCC CACATCCATA CTCAAGATAT GATGCATACT GTTTTAAAGC AAATCTGTCT
    TTGGGGTTTG ATGAAGATAT GCTGAATGCT ACAGACATAA CAATGCTGCA GAGACCTGGT
    ATCAAATTTC GTCCAATTGT CCCTCCCTTG TTTGTGGACA TGTCTGGATC TGGATCTGGA
    TATGCATCTG CATCTACATC TGGATCAGGC TCAGACTCAG ATTCAGCCTC AGGCTCGGCA
    TCAGGTGTTC CTGATTCTAG TTCCGGTTAC ATATCAGGAA GCGGTATAGC CAGTGGTAGT
    GCATCCGGTT CTGGCCAAAG TGGTGATGGA TCAGGTTTCA TTGTGAGGTA CCAAGAAGGT
    ACAGATGCAC TCATGTGGTC TTCTGCCTCA GGTAGTGCTT CAGAGGCTAA AGATGGCAGT
    GGTAGCGGTA TTATCATTAC TTCGGGAATA CAATCAGGGG ACCAAGAATC AGGAGAGGAG
    TCTGGACACT TTGAAGTGGC TAGTGGTCTT CCATCTGGAC TTGGCAGTGG AATTAGTGGT
    TCAGGAACAA GTGGGTTCTT CAGTGGTGAA GAATCAAGTG GATCAGGCTT CTTCAGTGTG
    TCATTTGTAG ACACAAAAAT GATTGACCTT ACTCCAAGAG CCTCTGGAGA GCAGGAATTG
    TCTGGTTTTT ACACTGCTGA AAGTGGGTTT CCATCTGGTT CTGGATCTGG TTCTGGCTTT
    GAAACTGGTT CTGCCATTGG ATTTGAATCA AACTCTGCCC TTGGATCTGG TTCTGGCTTT
    GAAACTGGCT CTGCCATTGG ATTTGAATCA AACTCTGCCT CTGCTTTTGG ATCTGGCTCT
    TCCTCTGGAT TTGAATCTAG CTCTGCCTTT GGATTTGGAT CTGGCTCTGC CTCTGGATTT
    GGATCTGGCA TTGCCTCTGG AATTGAATCT AGCCCTGCCT CTGCATTTGG ATCAAGCTCT
    GCTTCTGGAT TTGGATCTAG CTCTGCCATT GGGTTAGAAT CAAGCTCAGC CTCTGTCTTT
    AAATCCGTCT CTGTCACTGG AACATCAGGA TATCCTAATC ATCCTTCAGG CGAGGTGTCT
    GAAAACATTC AGAGACAAGA TGGAGAGATG ATTATTTTTA CAGATGATGA GGTCACTGAG
    TTGACGTTAA GGCCCACAGC TGGAACAGAA CAGGGTCGTG GATCGGTAGA GATCAGTGGT
    GAGGGCAGTA CTCCAGAACC TTACACAGAA GATTCCATAT CAGTTTCAAA TAGTACTTCA
    CATGAATCAA ATGACCTCCT ACCAGAAAGA GATGAGAAAA TTAGTGTCAC CATGGAGGCT
    TTTGATGTGA CTGACAAGTC ACCTCTGATT ACCACCCCAA CTAACATCCC TGTACTCACC
    ACTCCATCTT CAGCTTCACT TCAAATGCCA AAATCGACAA TGAATCCATC TGTTAATGAA
    GCTCCATCTG ACCCCTGTGA TCCAAACCCC TGTGGTCAAG GTCTATGCTC TTTACAAGAT
    GGAGTTGCTT TATGTCAGTG TCATTCAGGA TTCAGTGGAG AAAACTGCTC AGTGTTGGTA
    CAGGGATGTG CTGAAGGGTG GATGGAGTTC ATGGGCAGCT GTTACATTCA TTTTGATGAG
    CGTGAGACTT GGACCAGTGC TGAGCAACAC TGTCAAGAGC TAAACTCTCA CCTAGTTAGC
    ATATCCTCCC AACAAGAACA AGAGTTTGTA AAAACTCAAG CTCAAGATTA TCAGTGGATA
    GGGCTAAATG ATAAGGATGT GCAAAACGAG TTCCGTTGGA CAGATGGAAG TCCTTTGGAA
    TATGAAAACT GGAGACCCAA TCAGCCAGAT AATTACTTTA GCACAGGAGA AGATTGTGTT
    GTAATGATAT GGCATGAGAA TGGCCAGTGG AATGATGTAC CTTGCAACTA CCATCTTCCT
    TTCACTTGCA AGAGTGAACC TGTCACATGT TCAACACCTC CTGAGGTGCA GAATGCACGG
    ATGCTTGGGA ACAGCAAAGA TCGCTACCCA GTTAATTCTT TCATTCGATA CCAGTGTAAC
    AGTGGGTTTA CACAGCGCCA CCTTTCTGTG GTGCGCTGTT TACCAGATGG TCAGTGGGAA
    AAGCCTCGAG TTGAATGCAT AAGGGGCATA GAAAACAACA GGCTAAGGAA AAGATCCCTG
    TATATACGTC CCAAGGCAGT CAACAGTCGG ACGTGGAGGA AGGTCCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326217.1
    CDS187..4317
    Translation

    Target ORF information:

    RefSeq Version XM_021470542.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio aggrecan b (acanb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTTGA TTTGGCTCCT GCTGTATATA TGCACCCATG GCATTTCTGG CATGATATTA 
    TTTGGAGACA TGGCTGATCA AGACAGCACC CTCAGTGTGA GCATCCCTGT GGAACAGCCA
    TTGCGTCCCC TACTTGGGGG GAGCATGGTT ATCCCCTGCT ACTTTCAGGA CATCACCCTC
    CAGGACCCTG GCACCCTGAC CTCCCCTACC AATGCCCCAT TGTCCCAGCG CATTAAATGG
    AGTCATATCC ACAAGGGCAA AATATCCCTT ATACTGGTTG CCACTGAGGG TCAGGTGCAG
    GTTGAGACAG AGTACTTGGA CAGGGTTCAT CTGGTAAACT ACCCCATGGT GCCAAGTGAT
    GCTACCATTG AAATCTCAGA ACTCCGTTCC CATGATTCTG GCACCTACCG ATGTGAGGTC
    ATGCAGGGGA TTGACGATAA CTATGATTCG GTGGAAATGC AAATACAAGG CATTGTATTT
    CACTACCGAG CAATTTCTAC CCGATACACA TTAACCTTTG AAATGGCAAA AGCAGCCTGC
    ATTCAGAACA GTGCTGTTAT CGCAACTCCA GCACAGTTGC AAGCTGCCTA TGATGATGGA
    TTCCATCAGT GTGATGCTGG CTGGCTTTCA GACCAAACCG TGAGATATCC TATCCATGAA
    CCAAGGGAGC CTTGTTATGG AGACAAAGAT AATTTCCCAG GTGTTCGAAC ATATGGAGTC
    CGAGACGTCA ATGAAACCTA TGATGTGTAC TGTTTTGCAG AGAAAATGTC AGGCAGAGTG
    TTCTACTCTA TGTCTGTGGA AAAGTTCACC TTTGCAGATG CTGAAGACCA ATGTGCAAAG
    TTAGGAGCCC AATTAGCTAC CACAGGACAG CTGTACCTAG CATGGAAGAC TGGCATGGAT
    GTGTGCAACG CTGGCTGGCT TGCAGACAGG AGTGTGCGGT ACCCTATAAA TATAGCTAGA
    CCCCAATGTG GTGGGGGGCT TTTGGGAGTG CGAACAGTTT ACCTGTTTCC AAATCAGACT
    GGTTATCCAT ACCCAGACTC CCGCTATGAT GCAATCTGTT ATGAAGATAA AGAAGATAAG
    GTCTTAATAA AGACCACGCC ATTTCCAGAA ATATACACTA CCACCGAGTC AGTGTTAACA
    GTGGCTACAG TTACATCAAG CTCTTCAGAT TATACAGAAC AAGTGACCAC TGAAGGAGAG
    GTTCGTGGCC AAATATTGAC AGATGAGCCT TTTAACACAA CAAGCACAGA AAGTCCCCTA
    TCTCCACCTC TCAACATAAC TGATGTTGAG GAAGACATCA TCAATGTGGC CACAGCTCCA
    CCTTCCCTGG AACATGCAAT TCCCAGTATC AATGTCACTG CTAAAATCAA GGGAGTGGTA
    TTCCACTATC GTGCTGAATC TGGGCGCTAT GCTTACACTT ATGAGGAAGC ACAAGATGCT
    TGTCAGAAAC TGGGAGCAGT CATTGCCACT CCTGAGCTCC TTCAGGCTGC TTATGAAGCT
    GGCCTTCATC AATGCAGTGC AGGTTGGCTA CAAGATCAAA CTGTCAGATA TCCAATTGTG
    CATCCTCGAA AGAAATGCTC TGGAGATAGT GAGGATGTCC CTGGTGTACG CTCTTACAAT
    GTTAGGCCTG CACATGAACA ATATGATGTG TACTGTTACA TGGATCAAAT AAAGGGAGAG
    ATCTTCCATG TGAGCTCACT AGCTGGCTTT ACCTACTATG AAGCTGTTGC CCATTGCAGA
    AAACTGAGTT CTACTCTTGC CTCAACTGGA GAACTCTATG CAGCGTGGAA TCAGGGTTTC
    CACAAGTGCA GCCCTGGATG GCTGGCTGAC CGCAGTGTCC GCTACCCTGT ACGTAGCCCA
    GACTTTGATT GTGGTGGAAA CAAAACTGGA GTCCAGACAA TTTATGCCCA ACCAAACCAA
    ACAGGCTCCC CACATCCATA CTCAAGATAT GATGCATACT GTTTTAAAGC AAATCTGTCT
    TTGGGGTTTG ATGAAGATAT GCTGAATGCT ACAGACATAA CAATGCTGCA GAGACCTGGT
    ATCAAATTTC GTCCAATTGT CCCTCCCTTG TTTGTGGACA TGTCTGGATC TGGATCTGGA
    TATGCATCTG CATCTACATC TGGATCAGGC TCAGACTCAG ATTCAGCCTC AGGCTCGGCA
    TCAGGTGTTC CTGATTCTAG TTCCGGTTAC ATATCAGGAA GCGGTATAGC CAGTGGTAGT
    GCATCCGGTT CTGGCCAAAG TGGTGATGGA TCAGGTTTCA TTGTGAGGTA CCAAGAAGGT
    ACAGATGCAC TCATGTGGTC TTCTGCCTCA GGTAGTGCTT CAGAGGCTAA AGATGGCAGT
    GGTAGCGGTA TTATCATTAC TTCGGGAATA CAATCAGGGG ACCAAGAATC AGGAGAGGAG
    TCTGGACACT TTGAAGTGGC TAGTGGTCTT CCATCTGGAC TTGGCAGTGG AATTAGTGGT
    TCAGGAACAA GTGGGTTCTT CAGTGGTGAA GAATCAAGTG GATCAGGCTT CTTCAGTGTG
    TCATTTGTAG ACACAAAAAT GATTGACCTT ACTCCAAGAG CCTCTGGAGA GCAGGAATTG
    TCTGGTTTTT ACACTGCTGA AAGTGGGTTT CCATCTGGTT CTGGATCTGG TTCTGGCTTT
    GAAACTGGTT CTGCCATTGG ATTTGAATCA AACTCTGCCC TTGGATCTGG TTCTGGCTTT
    GAAACTGGCT CTGCCATTGG ATTTGAATCA AACTCTGCCT CTGCTTTTGG ATCTGGCTCT
    TCCTCTGGAT TTGAATCTAG CTCTGCCTTT GGATTTGGAT CTGGCTCTGC CTCTGGATTT
    GGATCTGGCA TTGCCTCTGG AATTGAATCT AGCCCTGCCT CTGCATTTGG ATCAAGCTCT
    GCTTCTGGAT TTGGATCTAG CTCTGCCATT GGGTTAGAAT CAAGCTCAGC CTCTGTCTTT
    AAATCCGTCT CTGTCACTGG AACATCAGGA TATCCTAATC ATCCTTCAGG CGAGGTGTCT
    GAAAACATTC AGAGACAAGA TGGAGAGATG ATTATTTTTA CAGATGATGA GGTCACTGAG
    TTGACGTTAA GGCCCACAGC TGGAACAGAA CAGGGTCGTG GATCGGTAGA GATCAGTGGT
    GAGGGCAGTA CTCCAGAACC TTACACAGAA GATTCCATAT CAGTTTCAAA TAGTACTTCA
    CATGAATCAA ATGACCTCCT ACCAGAAAGA GATGAGAAAA TTAGTGTCAC CATGGAGGCT
    TTTGATGTGA CTGACAAGTC ACCTCTGATT ACCACCCCAA CTAACATCCC TGTACTCACC
    ACTCCATCTT CAGCTTCACT TCAAATGCCA AAATCGACAA TGAATCCATC TGTTAATGAA
    GCTCCATCTG ACCCCTGTGA TCCAAACCCC TGTGGTCAAG GTCTATGCTC TTTACAAGAT
    GGAGTTGCTT TATGTCAGTG TCATTCAGGA TTCAGTGGAG AAAACTGCTC AGTGTTGGTA
    CAGGGATGTG CTGAAGGGTG GATGGAGTTC ATGGGCAGCT GTTACATTCA TTTTGATGAG
    CGTGAGACTT GGACCAGTGC TGAGCAACAC TGTCAAGAGC TAAACTCTCA CCTAGTTAGC
    ATATCCTCCC AACAAGAACA AGAGTTTGTA AAAACTCAAG CTCAAGATTA TCAGTGGATA
    GGGCTAAATG ATAAGGATGT GCAAAACGAG TTCCGTTGGA CAGATGGAAG TCCTTTGGAA
    TATGAAAACT GGAGACCCAA TCAGCCAGAT AATTACTTTA GCACAGGAGA AGATTGTGTT
    GTAATGATAT GGCATGAGAA TGGCCAGTGG AATGATGTAC CTTGCAACTA CCATCTTCCT
    TTCACTTGCA AGAGTGAACC TGTCACATGT TCAACACCTC CTGAGGTGCA GAATGCACGG
    ATGCTTGGGA ACAGCAAAGA TCGCTACCCA GTTAATTCTT TCATTCGATA CCAGTGTAAC
    AGTGGGTTTA CACAGCGCCA CCTTTCTGTG GTGCGCTGTT TACCAGATGG TCAGTGGGAA
    AAGCCTCGAG TTGAATGCAT AAGGGGCATA GAAAACAACA GGCTAAGGAA AAGATCCCTG
    TATATACGTC CCAAGGCAGT CAACAGTCGG ACGTGGAGGA AGGTCCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146385
    Clone ID Related Accession (Same CDS sequence) XM_021473853.1
    Accession Version XM_021473853.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product aggrecan core protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395197.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTTGA TTTGGCTCCT GCTGTATATA TGCACCCATG GCATTTCTGG CATGATATTA 
    TTTGGAGACA TGGCTGATCA AGACAGCACC CTCAGTGTGA GCATCCCTGT GGAACAGCCA
    TTGCGTCCCC TACTTGGGGG GAGCATGGTT ATCCCCTGCT ACTTTCAGGA CATCACCCTC
    CAGGACCCTG GCACCCTGAC CTCCCCTACC AATGCCCCAT TGTCCCAGCG TATTAAATGG
    AGTCATATCC ATAAGGGCAA AATATCCCTT ATACTGGTTG CTACTGAGGG TCAGGTGCAG
    GTTGAGACAG AGTACTTGGA CAGGGTTCAT CTGGTAAACT ACCCCATGGT GCCAAGTGAT
    GCTACCATTG AAATCTCAGA ACTCCGTTCC CATGATTCTG GCACCTACCG ATGTGAGGTC
    ATGCAGGGGA TTGACGATAA CTATGATTCG GTGGAAATGC AAATACAAGG CATTGTATTT
    CACTACCGAG CAATTTCTAC CCGATACACA TTAACCTTTG AAATGGCAAA AGCAGCCTGC
    ATTCAGAACA GTGCTGTTAT CGCAACTCCA GCACAGTTGC AAGCTGCCTA TGATGATGGA
    TTCCATCAGT GTGATGCTGG CTGGCTTTCA GACCAAACCG TGAGATATCC TATCCATGAA
    CCAAGGGAGC CTTGTTATGG AGACAAAGAT AATTTCCCAG GTGTTCGAAC ATATGGAGTC
    CGAGACGTCA ATGAAACCTA TGATGTGTAC TGTTTTGCAG AGAAAATGTC AGGCAGAGTG
    TTCTACTCTA TGTCCGTGGA AAAGTTCACC TTTGCAGATG CTGAAGACCA ATGTGCAAAG
    TTAGGAGCCC AATTAGCTAC CACAGGGCAG CTGTACCTAG CATGGAAGAC TGGCATGGAT
    GTGTGCAATG CTGGCTGGCT TGCAGACAGG AGTGTGCGGT ACCCTATAAA TATAGCTAGA
    CCCCAATGTG GTGGGGGGCT TTTGGGAGTG CGAACAGTTT ACCTGTTTCC AAATCAGACT
    GGTTATCCAT ACCCAGACTC CCGCTATGAT GCAATCTGTT ATGAAGATAA AGAAGATAAG
    GTCTTAATAA AGACCACGCC ATTTCCAGAA ATATACACTA CCACCGAGTC AGTGTTAACA
    GTGGCTACAG TTACATCAAG CTCTTCAGAT TATACAGAAC AAGTGACCAC TGAAGGAGAG
    GTCCGTGGCC AAATATTGAC AGATGAGCCT TTTAACACAA CAAGCACAGA AAGTCCCCTA
    TCTCCACCTC TCAACATAAC TGATGTTGAG GAAGACATCA TCAATGTGGC CACAGCTCCA
    CCTTCCCTGG AACATGCAAT TCCCAGTATC AATGTCACTG CTAAAATCAA GGGAGTGGTA
    TTCCACTATC GTGCTGAATC TGGGCGCTAT GCTTACACTT ATGAGGAAGC ACAAGATGCT
    TGTCAGAAAC TGGGAGCAGT CATTGCCACT CCTGAGCTCC TTCAGGCTGC TTATGAAGCT
    GGCCTTCATC AATGCAGTGC AGGTTGGCTA CAAGATCAAA CTGTCAGATA TCCAATTGTG
    CATCCTCGAA AGAAATGCTC TGGAGATAGT GAGGATGTCC CTGGTGTACG CTCTTACAAT
    GTTAGGCCTG CACATGAACA ATATGATGTG TACTGTTACA TGGACCAAAT AAAGGGGGAG
    ATCTTCCATG TGAGCTCACT AGCTGGCTTT ACCTACTATG AAGCTGTTGC CCATTGCAGA
    AAACTGAGTT CTACTCTTGC CTCAACTGGA GAACTCTATG CAGCGTGGAA TCAGGGTTTC
    CACAAGTGCA GCCCAGGATG GCTGGCTGAC CGCAGTGTCC GCTACCCTGT AGGTAGCCCA
    GACTTTGATT GTGGTGGAAA CAAAACTGGA GTCCAGACAA TTTATGCCCA ACCAAACCAA
    ACAGGCTCCC CACATCCATA CTCAAGATAT GATGCATACT GTTTTAAAGC AAATCTGTCT
    TTGGGGTTTG ATGAAGATAT GCTGAATGCT ACAGACATAA CAATGCTGCA GAGACCTGTT
    CATCCAATTG TCCCTCCCTT GTTTGTGGAC ATGTCTGGAT CTGGATCTGG ATCTGGATAT
    GCATCTGCAT CTACATCTGG ATCAGGCTCA GATTCAGCCT CAGGCTCGGC ATCAGGTGTT
    CCTGATTCTA GTTCCGGTTA CATTTCAGGA AGCGGTATAG CCAGTGGTAG TGCATCCGGT
    TCTGGCCAAA GTGGTGATGG ATCAGGTTTC CTTGTGAGGT ACCAAGAAGG TACAGATGCA
    CTCATGTGGT CTTCTGCCTC AGGTAGTGCT TCAGAGGCTA AAGATGGCAG TGGTAGCGGT
    ATTATCATTA CTTCGGGAAT ACAATCAGGG GACCAAGAAT CAGGAGAGGA GTCTGGACAC
    TTTGCAGTGG CTAGTGGTCT TCCATCTGGA CTTGGCAGTG GAATTAGTGG TTCAGGAACA
    AGTGGGTTCT TCAGTGGTGA AGAATCAAGT GGATCAGGCT TCTTCAGTGT GTCATTTGTA
    GACACAAAAA TGATTGACCT TACTGCAAGA GCCTTTGGAG AGCAGGAATT GTCTGGTTTT
    TACACTGCTG AAAGTGGGTT TCCATCTGGT TCTGGATCTT TTTCTGGCTT TGAAACTGGT
    TCTGCCATTG GATTTGAATC AAACTCTGCC TTTGGATCTG GTTCTGGCTT TGAAACTGGC
    TCTGCTATTG GATTTGAATC AAACTCTGCC TCTGCTTTTG GATCTGGCTC TTCCTCTGGA
    TTTGAATCTA GCTCTGCCTT TGGATTTGGA TCTGGCTCTG CCTCTGGATT TGGATCTGGC
    ATTGCCTCTG GAATTGAATC TAGCCCTGCC TCTGCATTTG GATCAGGCTC TGCTTCTGGA
    TTTGGATCTA GCTCTGCCAT TGGGTTAGAA TCAAGCTCAG CCTCTGGCTT TAAATCGGTC
    TCTGTCACTG GAACATCAGG ATATCCTAAT CATCCTTCAG GCGAGGTGTC TGAAAACATT
    CAGAGACAAG ATGGAGAGAT GATTATTTTT ACAGATGATG AGGTCACTGA GTTGACGTTA
    AGGCCCACAG CTGGAACAGA ACAGGGTCGT GGATCGGTAG AGATCAGTGG TGAGGGCAGT
    ACTCCAGAAC CTTACACAGA AGATTCCATA TCAGTTTCAA ATAGTACTTC ACATGAATCA
    AATGACCTCC TACCAGAAAG AGATGAGAAA ATTAGTGTCA CCATGGAGGC TTTTGATGTG
    ACTGACAAGT CACCTCTGAT TACCACCCCA ACTAACATCC CTGTACTCAC CACTCCATCT
    TCAGCTTCAC TTCAAATGCC AAAATCGACA ATGAATCCAT CTGTTAATGA AGCTCCATTT
    GACCCCTGTG ATCCAAACCC CTGTGGTCAA GGTCTATGTT CTTTACAAGA TGGAGTTGCT
    TTATGTCAAT GTCATTCAGG ATTCAGTGGA GAAAACTGCT CAATGTTGGT ACAGGGATGT
    GCTGAAGGGT GGATGGAGTT CATGGGCAGC TGTTACATTC ATTTTGATGA GCGTGAGACT
    TGGACCAGTG CTGAGCAACA CTGTCAAGAG CTAAACTCTC ACCTAGTTAG CATATCCTCC
    CAACAAGAAC AAGAGTTTGT AAAAACTCAA GCTCAAGATT ATCAGTGGAT CGGGCTAAAT
    GATAAGGATG TGCAAAACGA GTTCCGTTGG ACAGATGGAA GTCCTTTGGA ATATGAAAAC
    TGGAGACCCA ATCAGCCAGA TAATTACTTT AGCACAGGAG AAGATTGTGT TGTAATGATA
    TGGCATGAGA ATGGCCAGTG GAATGATGTA CCTTGCAACT ACCATCTTCC TTTCACTTGC
    AAGAGTGGAC CTGTCACATG TACAACGCCT CCTGAGGTGC AGAATGCACG GATGCTTGGG
    AACAGCAAAG ATCGCTACCC AGTTAATTCT TTCATTCGAT ACCAGTGTAA CAGTGGGTTT
    ACACAGCGCC ACCTTTCTGT GGTGCGCTGT TTACCAGATG GTCAGTGGGA AAAGCCTCGA
    GTGGAATGCA TAAGGGGCAT AGAAAACAAC AGGCTAAGGA AAAGATCCCT GTATATACGT
    CCCAAGGCAG TCAACAGTCG GACGTGGAGG AAGGTCCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021329528.1
    CDS185..4306
    Translation

    Target ORF information:

    RefSeq Version XM_021473853.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio aggrecan b (acanb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473853.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTTGA TTTGGCTCCT GCTGTATATA TGCACCCATG GCATTTCTGG CATGATATTA 
    TTTGGAGACA TGGCTGATCA AGACAGCACC CTCAGTGTGA GCATCCCTGT GGAACAGCCA
    TTGCGTCCCC TACTTGGGGG GAGCATGGTT ATCCCCTGCT ACTTTCAGGA CATCACCCTC
    CAGGACCCTG GCACCCTGAC CTCCCCTACC AATGCCCCAT TGTCCCAGCG TATTAAATGG
    AGTCATATCC ATAAGGGCAA AATATCCCTT ATACTGGTTG CTACTGAGGG TCAGGTGCAG
    GTTGAGACAG AGTACTTGGA CAGGGTTCAT CTGGTAAACT ACCCCATGGT GCCAAGTGAT
    GCTACCATTG AAATCTCAGA ACTCCGTTCC CATGATTCTG GCACCTACCG ATGTGAGGTC
    ATGCAGGGGA TTGACGATAA CTATGATTCG GTGGAAATGC AAATACAAGG CATTGTATTT
    CACTACCGAG CAATTTCTAC CCGATACACA TTAACCTTTG AAATGGCAAA AGCAGCCTGC
    ATTCAGAACA GTGCTGTTAT CGCAACTCCA GCACAGTTGC AAGCTGCCTA TGATGATGGA
    TTCCATCAGT GTGATGCTGG CTGGCTTTCA GACCAAACCG TGAGATATCC TATCCATGAA
    CCAAGGGAGC CTTGTTATGG AGACAAAGAT AATTTCCCAG GTGTTCGAAC ATATGGAGTC
    CGAGACGTCA ATGAAACCTA TGATGTGTAC TGTTTTGCAG AGAAAATGTC AGGCAGAGTG
    TTCTACTCTA TGTCCGTGGA AAAGTTCACC TTTGCAGATG CTGAAGACCA ATGTGCAAAG
    TTAGGAGCCC AATTAGCTAC CACAGGGCAG CTGTACCTAG CATGGAAGAC TGGCATGGAT
    GTGTGCAATG CTGGCTGGCT TGCAGACAGG AGTGTGCGGT ACCCTATAAA TATAGCTAGA
    CCCCAATGTG GTGGGGGGCT TTTGGGAGTG CGAACAGTTT ACCTGTTTCC AAATCAGACT
    GGTTATCCAT ACCCAGACTC CCGCTATGAT GCAATCTGTT ATGAAGATAA AGAAGATAAG
    GTCTTAATAA AGACCACGCC ATTTCCAGAA ATATACACTA CCACCGAGTC AGTGTTAACA
    GTGGCTACAG TTACATCAAG CTCTTCAGAT TATACAGAAC AAGTGACCAC TGAAGGAGAG
    GTCCGTGGCC AAATATTGAC AGATGAGCCT TTTAACACAA CAAGCACAGA AAGTCCCCTA
    TCTCCACCTC TCAACATAAC TGATGTTGAG GAAGACATCA TCAATGTGGC CACAGCTCCA
    CCTTCCCTGG AACATGCAAT TCCCAGTATC AATGTCACTG CTAAAATCAA GGGAGTGGTA
    TTCCACTATC GTGCTGAATC TGGGCGCTAT GCTTACACTT ATGAGGAAGC ACAAGATGCT
    TGTCAGAAAC TGGGAGCAGT CATTGCCACT CCTGAGCTCC TTCAGGCTGC TTATGAAGCT
    GGCCTTCATC AATGCAGTGC AGGTTGGCTA CAAGATCAAA CTGTCAGATA TCCAATTGTG
    CATCCTCGAA AGAAATGCTC TGGAGATAGT GAGGATGTCC CTGGTGTACG CTCTTACAAT
    GTTAGGCCTG CACATGAACA ATATGATGTG TACTGTTACA TGGACCAAAT AAAGGGGGAG
    ATCTTCCATG TGAGCTCACT AGCTGGCTTT ACCTACTATG AAGCTGTTGC CCATTGCAGA
    AAACTGAGTT CTACTCTTGC CTCAACTGGA GAACTCTATG CAGCGTGGAA TCAGGGTTTC
    CACAAGTGCA GCCCAGGATG GCTGGCTGAC CGCAGTGTCC GCTACCCTGT AGGTAGCCCA
    GACTTTGATT GTGGTGGAAA CAAAACTGGA GTCCAGACAA TTTATGCCCA ACCAAACCAA
    ACAGGCTCCC CACATCCATA CTCAAGATAT GATGCATACT GTTTTAAAGC AAATCTGTCT
    TTGGGGTTTG ATGAAGATAT GCTGAATGCT ACAGACATAA CAATGCTGCA GAGACCTGTT
    CATCCAATTG TCCCTCCCTT GTTTGTGGAC ATGTCTGGAT CTGGATCTGG ATCTGGATAT
    GCATCTGCAT CTACATCTGG ATCAGGCTCA GATTCAGCCT CAGGCTCGGC ATCAGGTGTT
    CCTGATTCTA GTTCCGGTTA CATTTCAGGA AGCGGTATAG CCAGTGGTAG TGCATCCGGT
    TCTGGCCAAA GTGGTGATGG ATCAGGTTTC CTTGTGAGGT ACCAAGAAGG TACAGATGCA
    CTCATGTGGT CTTCTGCCTC AGGTAGTGCT TCAGAGGCTA AAGATGGCAG TGGTAGCGGT
    ATTATCATTA CTTCGGGAAT ACAATCAGGG GACCAAGAAT CAGGAGAGGA GTCTGGACAC
    TTTGCAGTGG CTAGTGGTCT TCCATCTGGA CTTGGCAGTG GAATTAGTGG TTCAGGAACA
    AGTGGGTTCT TCAGTGGTGA AGAATCAAGT GGATCAGGCT TCTTCAGTGT GTCATTTGTA
    GACACAAAAA TGATTGACCT TACTGCAAGA GCCTTTGGAG AGCAGGAATT GTCTGGTTTT
    TACACTGCTG AAAGTGGGTT TCCATCTGGT TCTGGATCTT TTTCTGGCTT TGAAACTGGT
    TCTGCCATTG GATTTGAATC AAACTCTGCC TTTGGATCTG GTTCTGGCTT TGAAACTGGC
    TCTGCTATTG GATTTGAATC AAACTCTGCC TCTGCTTTTG GATCTGGCTC TTCCTCTGGA
    TTTGAATCTA GCTCTGCCTT TGGATTTGGA TCTGGCTCTG CCTCTGGATT TGGATCTGGC
    ATTGCCTCTG GAATTGAATC TAGCCCTGCC TCTGCATTTG GATCAGGCTC TGCTTCTGGA
    TTTGGATCTA GCTCTGCCAT TGGGTTAGAA TCAAGCTCAG CCTCTGGCTT TAAATCGGTC
    TCTGTCACTG GAACATCAGG ATATCCTAAT CATCCTTCAG GCGAGGTGTC TGAAAACATT
    CAGAGACAAG ATGGAGAGAT GATTATTTTT ACAGATGATG AGGTCACTGA GTTGACGTTA
    AGGCCCACAG CTGGAACAGA ACAGGGTCGT GGATCGGTAG AGATCAGTGG TGAGGGCAGT
    ACTCCAGAAC CTTACACAGA AGATTCCATA TCAGTTTCAA ATAGTACTTC ACATGAATCA
    AATGACCTCC TACCAGAAAG AGATGAGAAA ATTAGTGTCA CCATGGAGGC TTTTGATGTG
    ACTGACAAGT CACCTCTGAT TACCACCCCA ACTAACATCC CTGTACTCAC CACTCCATCT
    TCAGCTTCAC TTCAAATGCC AAAATCGACA ATGAATCCAT CTGTTAATGA AGCTCCATTT
    GACCCCTGTG ATCCAAACCC CTGTGGTCAA GGTCTATGTT CTTTACAAGA TGGAGTTGCT
    TTATGTCAAT GTCATTCAGG ATTCAGTGGA GAAAACTGCT CAATGTTGGT ACAGGGATGT
    GCTGAAGGGT GGATGGAGTT CATGGGCAGC TGTTACATTC ATTTTGATGA GCGTGAGACT
    TGGACCAGTG CTGAGCAACA CTGTCAAGAG CTAAACTCTC ACCTAGTTAG CATATCCTCC
    CAACAAGAAC AAGAGTTTGT AAAAACTCAA GCTCAAGATT ATCAGTGGAT CGGGCTAAAT
    GATAAGGATG TGCAAAACGA GTTCCGTTGG ACAGATGGAA GTCCTTTGGA ATATGAAAAC
    TGGAGACCCA ATCAGCCAGA TAATTACTTT AGCACAGGAG AAGATTGTGT TGTAATGATA
    TGGCATGAGA ATGGCCAGTG GAATGATGTA CCTTGCAACT ACCATCTTCC TTTCACTTGC
    AAGAGTGGAC CTGTCACATG TACAACGCCT CCTGAGGTGC AGAATGCACG GATGCTTGGG
    AACAGCAAAG ATCGCTACCC AGTTAATTCT TTCATTCGAT ACCAGTGTAA CAGTGGGTTT
    ACACAGCGCC ACCTTTCTGT GGTGCGCTGT TTACCAGATG GTCAGTGGGA AAAGCCTCGA
    GTGGAATGCA TAAGGGGCAT AGAAAACAAC AGGCTAAGGA AAAGATCCCT GTATATACGT
    CCCAAGGCAG TCAACAGTCG GACGTGGAGG AAGGTCCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29379
    Clone ID Related Accession (Same CDS sequence) XM_682950.6
    Accession Version XM_682950.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4122bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product aggrecan core protein
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_682950.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTTGA TTTGGCTCCT GCTGTATATA TGCACCCATG GCATTTCTGG CATGATATTA 
    TTTGGAGACA TGGCTGATCA AGACAGCACC CTCAGTGTGA GCATCCCTGT GGAACAGCCA
    TTGCGTCCCC TACTTGGGGG GAGCATGGTT ATCCCCTGCT ACTTTCAGGA CATCACCCTC
    CAGGACCCTG GCACCCTGAC CTCCCCTACC AATGCCCCAT TGTCCCAGCG CATTAAATGG
    AGTCATATCC ACAAGGGCAA AATATCCCTT ATACTGGTTG CCACTGAGGG TCAGGTGCAG
    GTTGAGACAG AGTACTTGGA CAGGGTTCAT CTGGTAAACT ACCCCATGGT GCCAAGTGAT
    GCTACCATTG AAATCTCAGA ACTCCGTTCC CATGATTCTG GCACCTACCG ATGTGAGGTC
    ATGCAGGGGA TTGACGATAA CTATGATTCG GTGGAAATGC AAATACAAGG CATTGTATTT
    CACTACCGAG CAATTTCTAC CCGATACACA TTAACCTTTG AAATGGCAAA AGCAGCCTGC
    ATTCAGAACA GTGCTGTTAT CGCAACTCCA GCACAGTTGC AAGCTGCCTA TGATGATGGA
    TTCCATCAGT GTGATGCTGG CTGGCTTTCA GACCAAACCG TGAGATATCC TATCCATGAA
    CCAAGGGAGC CTTGTTATGG AGACAAAGAT AATTTCCCAG GTGTTCGAAC ATATGGAGTC
    CGAGACGTCA ATGAAACCTA TGATGTGTAC TGTTTTGCAG AGAAAATGTC AGGCAGAGTG
    TTCTACTCTA TGTCTGTGGA AAAGTTCACC TTTGCAGATG CTGAAGACCA ATGTGCAAAG
    TTAGGAGCCC AATTAGCTAC CACAGGACAG CTGTACCTAG CATGGAAGAC TGGCATGGAT
    GTGTGCAACG CTGGCTGGCT TGCAGACAGG AGTGTGCGGT ACCCTATAAA TATAGCTAGA
    CCCCAATGTG GTGGGGGGCT TTTGGGAGTG CGAACAGTTT ACCTGTTTCC AAATCAGACT
    GGTTATCCAT ACCCAGACTC CCGCTATGAT GCAATCTGTT ATGAAGATAA AGAAGATAAG
    GTCTTAATAA AGACCACGCC ATTTCCAGAA ATATACACTA CCACCGAGTC AGTGTTAACA
    GTGGCTACAG TTACATCAAG CTCTTCAGAT TATACAGAAC AAGTGACCAC TGAAGGAGAG
    GTTCGTGGCC AAATATTGAC AGATGAGCCT TTTAACACAA CAAGCACAGA AAGTCCCCTA
    TCTCCACCTC TCAACATAAC TGATGTTGAG GAAGACATCA TCAATGTGGC CACAGCTCCA
    CCTTCCCTGG AACATGCAAT TCCCAGTATC AATGTCACTG CTAAAATCAA GGGAGTGGTA
    TTCCACTATC GTGCTGAATC TGGGCGCTAT GCTTACACTT ATGAGGAAGC ACAAGATGCT
    TGTCAGAAAC TGGGAGCAGT CATTGCCACT CCTGAGCTCC TTCAGGCTGC TTATGAAGCT
    GGCCTTCATC AATGCAGTGC AGGTTGGCTA CAAGATCAAA CTGTCAGATA TCCAATTGTG
    CATCCTCGAA AGAAATGCTC TGGAGATAGT GAGGATGTCC CTGGTGTACG CTCTTACAAT
    GTTAGGCCTG CACATGAACA ATATGATGTG TACTGTTACA TGGATCAAAT AAAGGGAGAG
    ATCTTCCATG TGAGCTCACT AGCTGGCTTT ACCTACTATG AAGCTGTTGC CCATTGCAGA
    AAACTGAGTT CTACTCTTGC CTCAACTGGA GAACTCTATG CAGCGTGGAA TCAGGGTTTC
    CACAAGTGCA GCCCTGGATG GCTGGCTGAC CGCAGTGTCC GCTACCCTGT ACGTAGCCCA
    GACTTTGATT GTGGTGGAAA CAAAACTGGA GTCCAGACAA TTTATGCCCA ACCAAACCAA
    ACAGGCTCCC CACATCCATA CTCAAGATAT GATGCATACT GTTTTAAAGC AAATCTGTCT
    TTGGGGTTTG ATGAAGATAT GCTGAATGCT ACAGACATAA CAATGCTGCA GAGACCTGTT
    CGTCCAATTG TCCCTCCCTT GTTTGTGGAC ATGTCTGGAT CTGGATCTGG ATATGCATCT
    GCATCTACAT CTGGATCAGG CTCAGACTCA GATTCAGCCT CAGGCTCGGC ATCAGGTGTT
    CCTGATTCTA GTTCCGGTTA CATATCAGGA AGCGGTATAG CCAGTGGTAG TGCATCCGGT
    TCTGGCCAAA GTGGTGATGG ATCAGGTTTC ATTGTGAGGT ACCAAGAAGG TACAGATGCA
    CTCATGTGGT CTTCTGCCTC AGGTAGTGCT TCAGAGGCTA AAGATGGCAG TGGTAGCGGT
    ATTATCATTA CTTCGGGAAT ACAATCAGGG GACCAAGAAT CAGGAGAGGA GTCTGGACAC
    TTTGAAGTGG CTAGTGGTCT TCCATCTGGA CTTGGCAGTG GAATTAGTGG TTCAGGAACA
    AGTGGGTTCT TCAGTGGTGA AGAATCAAGT GGATCAGGCT TCTTCAGTGT GTCATTTGTA
    GACACAAAAA TGATTGACCT TACTCCAAGA GCCTCTGGAG AGCAGGAATT GTCTGGTTTT
    TACACTGCTG AAAGTGGGTT TCCATCTGGT TCTGGATCTG GTTCTGGCTT TGAAACTGGT
    TCTGCCATTG GATTTGAATC AAACTCTGCC CTTGGATCTG GTTCTGGCTT TGAAACTGGC
    TCTGCCATTG GATTTGAATC AAACTCTGCC TCTGCTTTTG GATCTGGCTC TTCCTCTGGA
    TTTGAATCTA GCTCTGCCTT TGGATTTGGA TCTGGCTCTG CCTCTGGATT TGGATCTGGC
    ATTGCCTCTG GAATTGAATC TAGCCCTGCC TCTGCATTTG GATCAAGCTC TGCTTCTGGA
    TTTGGATCTA GCTCTGCCAT TGGGTTAGAA TCAAGCTCAG CCTCTGTCTT TAAATCCGTC
    TCTGTCACTG GAACATCAGG ATATCCTAAT CATCCTTCAG GCGAGGTGTC TGAAAACATT
    CAGAGACAAG ATGGAGAGAT GATTATTTTT ACAGATGATG AGGTCACTGA GTTGACGTTA
    AGGCCCACAG CTGGAACAGA ACAGGGTCGT GGATCGGTAG AGATCAGTGG TGAGGGCAGT
    ACTCCAGAAC CTTACACAGA AGATTCCATA TCAGTTTCAA ATAGTACTTC ACATGAATCA
    AATGACCTCC TACCAGAAAG AGATGAGAAA ATTAGTGTCA CCATGGAGGC TTTTGATGTG
    ACTGACAAGT CACCTCTGAT TACCACCCCA ACTAACATCC CTGTACTCAC CACTCCATCT
    TCAGCTTCAC TTCAAATGCC AAAATCGACA ATGAATCCAT CTGTTAATGA AGCTCCATCT
    GACCCCTGTG ATCCAAACCC CTGTGGTCAA GGTCTATGCT CTTTACAAGA TGGAGTTGCT
    TTATGTCAGT GTCATTCAGG ATTCAGTGGA GAAAACTGCT CAGTGTTGGT ACAGGGATGT
    GCTGAAGGGT GGATGGAGTT CATGGGCAGC TGTTACATTC ATTTTGATGA GCGTGAGACT
    TGGACCAGTG CTGAGCAACA CTGTCAAGAG CTAAACTCTC ACCTAGTTAG CATATCCTCC
    CAACAAGAAC AAGAGTTTGT AAAAACTCAA GCTCAAGATT ATCAGTGGAT AGGGCTAAAT
    GATAAGGATG TGCAAAACGA GTTCCGTTGG ACAGATGGAA GTCCTTTGGA ATATGAAAAC
    TGGAGACCCA ATCAGCCAGA TAATTACTTT AGCACAGGAG AAGATTGTGT TGTAATGATA
    TGGCATGAGA ATGGCCAGTG GAATGATGTA CCTTGCAACT ACCATCTTCC TTTCACTTGC
    AAGAGTGAAC CTGTCACATG TTCAACACCT CCTGAGGTGC AGAATGCACG GATGCTTGGG
    AACAGCAAAG ATCGCTACCC AGTTAATTCT TTCATTCGAT ACCAGTGTAA CAGTGGGTTT
    ACACAGCGCC ACCTTTCTGT GGTGCGCTGT TTACCAGATG GTCAGTGGGA AAAGCCTCGA
    GTTGAATGCA TAAGGGGCAT AGAAAACAAC AGGCTAAGGA AAAGATCCCT GTATATACGT
    CCCAAGGCAG TCAACAGTCG GACGTGGAGG AAGGTCCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_688042.4
    CDS137..4258
    Translation

    Target ORF information:

    RefSeq Version XM_682950.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio aggrecan b (acanb), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_682950.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCTTTGA TTTGGCTCCT GCTGTATATA TGCACCCATG GCATTTCTGG CATGATATTA 
    TTTGGAGACA TGGCTGATCA AGACAGCACC CTCAGTGTGA GCATCCCTGT GGAACAGCCA
    TTGCGTCCCC TACTTGGGGG GAGCATGGTT ATCCCCTGCT ACTTTCAGGA CATCACCCTC
    CAGGACCCTG GCACCCTGAC CTCCCCTACC AATGCCCCAT TGTCCCAGCG CATTAAATGG
    AGTCATATCC ACAAGGGCAA AATATCCCTT ATACTGGTTG CCACTGAGGG TCAGGTGCAG
    GTTGAGACAG AGTACTTGGA CAGGGTTCAT CTGGTAAACT ACCCCATGGT GCCAAGTGAT
    GCTACCATTG AAATCTCAGA ACTCCGTTCC CATGATTCTG GCACCTACCG ATGTGAGGTC
    ATGCAGGGGA TTGACGATAA CTATGATTCG GTGGAAATGC AAATACAAGG CATTGTATTT
    CACTACCGAG CAATTTCTAC CCGATACACA TTAACCTTTG AAATGGCAAA AGCAGCCTGC
    ATTCAGAACA GTGCTGTTAT CGCAACTCCA GCACAGTTGC AAGCTGCCTA TGATGATGGA
    TTCCATCAGT GTGATGCTGG CTGGCTTTCA GACCAAACCG TGAGATATCC TATCCATGAA
    CCAAGGGAGC CTTGTTATGG AGACAAAGAT AATTTCCCAG GTGTTCGAAC ATATGGAGTC
    CGAGACGTCA ATGAAACCTA TGATGTGTAC TGTTTTGCAG AGAAAATGTC AGGCAGAGTG
    TTCTACTCTA TGTCTGTGGA AAAGTTCACC TTTGCAGATG CTGAAGACCA ATGTGCAAAG
    TTAGGAGCCC AATTAGCTAC CACAGGACAG CTGTACCTAG CATGGAAGAC TGGCATGGAT
    GTGTGCAACG CTGGCTGGCT TGCAGACAGG AGTGTGCGGT ACCCTATAAA TATAGCTAGA
    CCCCAATGTG GTGGGGGGCT TTTGGGAGTG CGAACAGTTT ACCTGTTTCC AAATCAGACT
    GGTTATCCAT ACCCAGACTC CCGCTATGAT GCAATCTGTT ATGAAGATAA AGAAGATAAG
    GTCTTAATAA AGACCACGCC ATTTCCAGAA ATATACACTA CCACCGAGTC AGTGTTAACA
    GTGGCTACAG TTACATCAAG CTCTTCAGAT TATACAGAAC AAGTGACCAC TGAAGGAGAG
    GTTCGTGGCC AAATATTGAC AGATGAGCCT TTTAACACAA CAAGCACAGA AAGTCCCCTA
    TCTCCACCTC TCAACATAAC TGATGTTGAG GAAGACATCA TCAATGTGGC CACAGCTCCA
    CCTTCCCTGG AACATGCAAT TCCCAGTATC AATGTCACTG CTAAAATCAA GGGAGTGGTA
    TTCCACTATC GTGCTGAATC TGGGCGCTAT GCTTACACTT ATGAGGAAGC ACAAGATGCT
    TGTCAGAAAC TGGGAGCAGT CATTGCCACT CCTGAGCTCC TTCAGGCTGC TTATGAAGCT
    GGCCTTCATC AATGCAGTGC AGGTTGGCTA CAAGATCAAA CTGTCAGATA TCCAATTGTG
    CATCCTCGAA AGAAATGCTC TGGAGATAGT GAGGATGTCC CTGGTGTACG CTCTTACAAT
    GTTAGGCCTG CACATGAACA ATATGATGTG TACTGTTACA TGGATCAAAT AAAGGGAGAG
    ATCTTCCATG TGAGCTCACT AGCTGGCTTT ACCTACTATG AAGCTGTTGC CCATTGCAGA
    AAACTGAGTT CTACTCTTGC CTCAACTGGA GAACTCTATG CAGCGTGGAA TCAGGGTTTC
    CACAAGTGCA GCCCTGGATG GCTGGCTGAC CGCAGTGTCC GCTACCCTGT ACGTAGCCCA
    GACTTTGATT GTGGTGGAAA CAAAACTGGA GTCCAGACAA TTTATGCCCA ACCAAACCAA
    ACAGGCTCCC CACATCCATA CTCAAGATAT GATGCATACT GTTTTAAAGC AAATCTGTCT
    TTGGGGTTTG ATGAAGATAT GCTGAATGCT ACAGACATAA CAATGCTGCA GAGACCTGTT
    CGTCCAATTG TCCCTCCCTT GTTTGTGGAC ATGTCTGGAT CTGGATCTGG ATATGCATCT
    GCATCTACAT CTGGATCAGG CTCAGACTCA GATTCAGCCT CAGGCTCGGC ATCAGGTGTT
    CCTGATTCTA GTTCCGGTTA CATATCAGGA AGCGGTATAG CCAGTGGTAG TGCATCCGGT
    TCTGGCCAAA GTGGTGATGG ATCAGGTTTC ATTGTGAGGT ACCAAGAAGG TACAGATGCA
    CTCATGTGGT CTTCTGCCTC AGGTAGTGCT TCAGAGGCTA AAGATGGCAG TGGTAGCGGT
    ATTATCATTA CTTCGGGAAT ACAATCAGGG GACCAAGAAT CAGGAGAGGA GTCTGGACAC
    TTTGAAGTGG CTAGTGGTCT TCCATCTGGA CTTGGCAGTG GAATTAGTGG TTCAGGAACA
    AGTGGGTTCT TCAGTGGTGA AGAATCAAGT GGATCAGGCT TCTTCAGTGT GTCATTTGTA
    GACACAAAAA TGATTGACCT TACTCCAAGA GCCTCTGGAG AGCAGGAATT GTCTGGTTTT
    TACACTGCTG AAAGTGGGTT TCCATCTGGT TCTGGATCTG GTTCTGGCTT TGAAACTGGT
    TCTGCCATTG GATTTGAATC AAACTCTGCC CTTGGATCTG GTTCTGGCTT TGAAACTGGC
    TCTGCCATTG GATTTGAATC AAACTCTGCC TCTGCTTTTG GATCTGGCTC TTCCTCTGGA
    TTTGAATCTA GCTCTGCCTT TGGATTTGGA TCTGGCTCTG CCTCTGGATT TGGATCTGGC
    ATTGCCTCTG GAATTGAATC TAGCCCTGCC TCTGCATTTG GATCAAGCTC TGCTTCTGGA
    TTTGGATCTA GCTCTGCCAT TGGGTTAGAA TCAAGCTCAG CCTCTGTCTT TAAATCCGTC
    TCTGTCACTG GAACATCAGG ATATCCTAAT CATCCTTCAG GCGAGGTGTC TGAAAACATT
    CAGAGACAAG ATGGAGAGAT GATTATTTTT ACAGATGATG AGGTCACTGA GTTGACGTTA
    AGGCCCACAG CTGGAACAGA ACAGGGTCGT GGATCGGTAG AGATCAGTGG TGAGGGCAGT
    ACTCCAGAAC CTTACACAGA AGATTCCATA TCAGTTTCAA ATAGTACTTC ACATGAATCA
    AATGACCTCC TACCAGAAAG AGATGAGAAA ATTAGTGTCA CCATGGAGGC TTTTGATGTG
    ACTGACAAGT CACCTCTGAT TACCACCCCA ACTAACATCC CTGTACTCAC CACTCCATCT
    TCAGCTTCAC TTCAAATGCC AAAATCGACA ATGAATCCAT CTGTTAATGA AGCTCCATCT
    GACCCCTGTG ATCCAAACCC CTGTGGTCAA GGTCTATGCT CTTTACAAGA TGGAGTTGCT
    TTATGTCAGT GTCATTCAGG ATTCAGTGGA GAAAACTGCT CAGTGTTGGT ACAGGGATGT
    GCTGAAGGGT GGATGGAGTT CATGGGCAGC TGTTACATTC ATTTTGATGA GCGTGAGACT
    TGGACCAGTG CTGAGCAACA CTGTCAAGAG CTAAACTCTC ACCTAGTTAG CATATCCTCC
    CAACAAGAAC AAGAGTTTGT AAAAACTCAA GCTCAAGATT ATCAGTGGAT AGGGCTAAAT
    GATAAGGATG TGCAAAACGA GTTCCGTTGG ACAGATGGAA GTCCTTTGGA ATATGAAAAC
    TGGAGACCCA ATCAGCCAGA TAATTACTTT AGCACAGGAG AAGATTGTGT TGTAATGATA
    TGGCATGAGA ATGGCCAGTG GAATGATGTA CCTTGCAACT ACCATCTTCC TTTCACTTGC
    AAGAGTGAAC CTGTCACATG TTCAACACCT CCTGAGGTGC AGAATGCACG GATGCTTGGG
    AACAGCAAAG ATCGCTACCC AGTTAATTCT TTCATTCGAT ACCAGTGTAA CAGTGGGTTT
    ACACAGCGCC ACCTTTCTGT GGTGCGCTGT TTACCAGATG GTCAGTGGGA AAAGCCTCGA
    GTTGAATGCA TAAGGGGCAT AGAAAACAAC AGGCTAAGGA AAAGATCCCT GTATATACGT
    CCCAAGGCAG TCAACAGTCG GACGTGGAGG AAGGTCCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.