acana cDNA ORF clone, Danio rerio(zebrafish)

The following acana gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the acana cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa13397 XM_005166485.4
Latest version!
Danio rerio aggrecan a (acana), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa13398 XM_681090.9
Latest version!
Danio rerio aggrecan a (acana), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa143513 XM_021477951.1
Latest version!
Danio rerio aggrecan a (acana), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
ODa13398 XM_681090.8 Danio rerio aggrecan a (acana), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa13397 XM_005166485.3 Danio rerio aggrecan a (acana), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa13397
    Clone ID Related Accession (Same CDS sequence) XM_005166485.3 , XM_005166485.4
    Accession Version XM_005166485.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product aggrecan core protein isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT
    TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA
    TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC
    TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT
    GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG
    CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA
    GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA
    AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA
    AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT
    CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA
    GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC
    CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC
    ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA
    ACAGCATTGG AGAAACAACT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166542.1
    CDS157..4800
    Translation

    Target ORF information:

    RefSeq Version XM_005166485.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio aggrecan a (acana), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166485.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT
    TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA
    TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC
    TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT
    GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG
    CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA
    GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA
    AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA
    AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT
    CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA
    GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC
    CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC
    ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA
    ACAGCATTGG AGAAACAACT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13397
    Clone ID Related Accession (Same CDS sequence) XM_005166485.3 , XM_005166485.4
    Accession Version XM_005166485.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product aggrecan core protein isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166485.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT
    TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA
    TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC
    TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT
    GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG
    CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA
    GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA
    AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA
    AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT
    CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA
    GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC
    CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC
    ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA
    ACAGCATTGG AGAAACAACT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166542.1
    CDS167..4810
    Translation

    Target ORF information:

    RefSeq Version XM_005166485.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio aggrecan a (acana), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166485.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGAGGTTGA TGTATGCCAT
    TCAAACCCTT GCGCCAATGG AGCCACCTGT GTGGAGGTAG AGGACACTTT CAAATGCTTA
    TGTCTGCCCA GCTACGAAGG AGACCGGTGT GAGACTGATT CACACAGCTG TGAGAAGGGC
    TGGACTAAGT TTCAGGGCAA CTGTTATTTA CATTTCTCGA AGAGAGAGAC CTGGCTGGAT
    GCTGAGCAAC GCTGTCGAGA CTTAAATGCT CACCTGGTGA GCATTAACAC TCCAGAGGAG
    CAAGCCTTCG TCAACTCAAA TGCACAAGAC TATCAGTGGA TTGGACTAAA TGATAAAACA
    GTGGAAAATG ACTTCCGCTG GTCAGATGGA ACCCAGCTGC AATTTGAGAA CTGGAGACCA
    AACCAGCCTG ACAATTATTT CAACTCTGAA GAGGATTGCG TTGTGATGAT CTGGCATGAA
    AATGGCCAGT GGAATGACGT GCCCTGCAAC TACCATCTGC CTTTTACATG CAAATCAGGT
    CCAGTGACAT GTGATAAACC TCCGAAAGTG GAGAATGCCA AAATGTTTGG AAACAAGAAA
    GAGCGTTACC AGGTTAATTC TATTATCAGA TACCAGTGCA GTGAAAACTT TACACAGCGC
    CACCCACCTG TGATCCGCTG CATGGCAGAC GGACAGTGGG AGAAGCCAAA AGTTCAATGC
    ATACCCAAGG TCAAATTTGG ACTTCACGAC AAATCTGTTC ATCACTCTCA TAAAGCTAGA
    ACAGCATTGG AGAAACAACT CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13398
    Clone ID Related Accession (Same CDS sequence) XM_681090.9 , XM_681090.8
    Accession Version XM_681090.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product aggrecan core protein isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_681090.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG
    AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG
    CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA
    GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT
    AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG
    AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG
    CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA
    TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC
    AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA
    CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT
    CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA
    GCTAGAACAG CATTGGAGAA ACAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_686182.6
    CDS168..4697
    Translation

    Target ORF information:

    RefSeq Version XM_681090.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio aggrecan a (acana), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_681090.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG
    AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG
    CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA
    GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT
    AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG
    AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG
    CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA
    TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC
    AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA
    CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT
    CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA
    GCTAGAACAG CATTGGAGAA ACAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13398
    Clone ID Related Accession (Same CDS sequence) XM_681090.9 , XM_681090.8
    Accession Version XM_681090.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product aggrecan core protein isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_681090.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG
    AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG
    CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA
    GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT
    AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG
    AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG
    CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA
    TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC
    AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA
    CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT
    CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA
    GCTAGAACAG CATTGGAGAA ACAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_686182.6
    CDS157..4686
    Translation

    Target ORF information:

    RefSeq Version XM_681090.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio aggrecan a (acana), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_681090.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGAAGGTA CCCCTATCAT TGCTCCCAGT
    CCTACAGAAC CTTCTGGAAC TGAGGAATCT GTGGCCAGCA GTTCTGGCGA TCTGTTGAGC
    AGTGGCAGTG GGGATCAGCC AAGTGGGTCA TCCGTTTCAA GTGGTGACTC CTCCGGGACA
    AGTAGCACTG ATGATCTTTT GACAAGTGGG AGCGGGGATC AGAGTGGAAG TGGCTCATCT
    GTTACAAGTG GAGACTATTC TGGAGCCAGT GGCACAGACA GTCTGTTCAG TAGTGGCAGT
    GGCGATCGGT CGAGTGGGTC ATTCAGTTCC AGTGGAGACT TCTCTGGAGC CAGTGGGACT
    GATGATTTGT TGGGCAGTGG GAGTGGGGAA CAGCCAAGTG GGTCATTTGT GTCAAGCGGA
    GACTTCTCTG GGGCCAGTGG AAGTGGGGAT ATGTCAAGTG GTTCTGGAAG CTCTTCAGGA
    TCTGGTAGTG GTTTAGATAT CTTAGTGACT TTTTCAGGGA TTGACACAGT TTTTTCAGGA
    GTTGGATCAA CTTCAGGGCA ACTTGAGGAG GCTGGGGAGG GAAGCACTGA GATTCTTACA
    TTCCAATTAG ATCAAATCTC TGGATCTGGA TCGGGATTAG GCAGTGGGTC AGGGTACAGC
    TCTGAAGAAA GTGGATCTAC CTCAGACCTT TCCAGCAGCT CAGGAGAGAG TGGTGAGAGT
    GGAGATCTTT CTGGCATCTC CTCAGGATCA GGGTTCAGTG AGGAGTCCTC AGGATTCAGT
    GGTTTTGCAT CAGGTATTTT TCCCTCTGGT GGATCCAGTG ACATTTCATT CATTGATGGC
    AGTGGAATCA TCATGGTGGA CAGGCCGTGG ATGAAGATAT CTTCATTTTT AACAGAACAA
    GAGCTGGGTA GTGGTCAAGT CGATTTCAGT GGATCAGGAC TTTTCTCAGG GTCTGGGGTG
    AGTGGTGATA TCTCTGGCTT AAGTGGTGAA ATCTCTGGAT CGAGTGGTGA TAGTTCAGGT
    ATAAGTAGTG ACATTTCAGG AATGAGTGGT GATATCTCAG GAATGAGTGG TGATATCTCA
    GGAATAAGTG GTGATATTAC AGGAATGAGT GGTGATCTCT CTGGTATGAG TAGCAGTGAC
    CACAGTGGCT CAGGGAGTGG TTTAGCATTT TCTGGGGTGA CATTTTTGGG GTCAGGCTTC
    ACTGATCTCA CAGAACAATC TCGTGAACAG GAAGCTTCTG GACTTGTGTT CTTTGGGTCT
    GGTGAGGGAA GTGGATTTAG ATCTGGAGGC TATGCAATTG AATCAGGGGA ACGATCAGGG
    GACTCGGCTA GTAGTGATGT CATCTTCTTC AGCAATAATG ACATGGTGGA AATGTCAAAC
    AAGCCACTTC AAGGAATGGA ATTAGGAAGA GGGGAAGTGG AATATAGTGG CACAGTCAGA
    CTGTCCTCAG GGAGTGGAGA TGACCAAAGT GTCTTTATAA GCGGGCATGA ATGGATCCCA
    GTCACTGCTA ATACACCATC TGTAAAATCC ACTGATCCTC CCCCAGCTGA ATTATTTGAT
    CAAGGTCCAT CAGACCTGTA CAGTGATACT GCAGGACACC TAGGTCCTTA TTTAAAACCA
    GAAGAGATCA TCAACAATCT TGACGCTGTT CCTTTGGCAG CTTTGTCTTC TACTACAGAA
    ACTGCCTCTT TACAGAGGCC TGCTGTCGTA GAACGACCTG CTGTGAATGA TTATCTTAAC
    CCATGTGAAC CAAACCCTTG TGGAGCTGGT GTGTGTTCAG TGAAGGATGG AGTGGGACTT
    TGTCACTGCC CACCTGGACT GCATGGAGAA GAGTGCCAGT TTGATTCACA CAGCTGTGAG
    AAGGGCTGGA CTAAGTTTCA GGGCAACTGT TATTTACATT TCTCGAAGAG AGAGACCTGG
    CTGGATGCTG AGCAACGCTG TCGAGACTTA AATGCTCACC TGGTGAGCAT TAACACTCCA
    GAGGAGCAAG CCTTCGTCAA CTCAAATGCA CAAGACTATC AGTGGATTGG ACTAAATGAT
    AAAACAGTGG AAAATGACTT CCGCTGGTCA GATGGAACCC AGCTGCAATT TGAGAACTGG
    AGACCAAACC AGCCTGACAA TTATTTCAAC TCTGAAGAGG ATTGCGTTGT GATGATCTGG
    CATGAAAATG GCCAGTGGAA TGACGTGCCC TGCAACTACC ATCTGCCTTT TACATGCAAA
    TCAGGTCCAG TGACATGTGA TAAACCTCCG AAAGTGGAGA ATGCCAAAAT GTTTGGAAAC
    AAGAAAGAGC GTTACCAGGT TAATTCTATT ATCAGATACC AGTGCAGTGA AAACTTTACA
    CAGCGCCACC CACCTGTGAT CCGCTGCATG GCAGACGGAC AGTGGGAGAA GCCAAAAGTT
    CAATGCATAC CCAAGGTCAA ATTTGGACTT CACGACAAAT CTGTTCATCA CTCTCATAAA
    GCTAGAACAG CATTGGAGAA ACAACTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143513
    Clone ID Related Accession (Same CDS sequence) XM_021477951.1
    Accession Version XM_021477951.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product aggrecan core protein isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGGTACCC CTATCATTGC TCCCAGTCCT
    ACAGAACCTT CTGGAACTGA GGAATCTGTG GCCAGCAGTT CTGGCGATCT GTTGAGCAGT
    GGCAGTGGGG ATCAGCCAAG TGGGTCATCC GTTTCAAGTG GTGACTCCTC CGGGACAAGT
    AGCACTGATG ATCTTTTGAC AAGTGGGAGC GGGGATCAGA GTGGAAGTGG CTCATCTGTT
    ACAAGTGGAG ACTATTCTGG AGCCAGTGGC ACAGACAGTC TGTTCAGTAG TGGCAGTGGC
    GATCGGTCGA GTGGGTCATT CAGTTCCAGT GGAGACTTCT CTGGAGCCAG TGGGACTGAT
    GATTTGTTGG GCAGTGGGAG TGGGGAACAG CCAAGTGGGT CATTTGTGTC AAGCGGAGAC
    TTCTCTGGGG CCAGTGGAAG TGGGGATATG TCAAGTGGTT CTGGAAGCTC TTCAGGATCT
    GGTAGTGGTT TAGATATCTT AGTGACTTTT TCAGGGATTG ACACAGTTTT TTCAGGAGTT
    GGATCAACTT CAGGGCAACT TGAGGAGGCT GGGGAGGGAA GCACTGAGAT TCTTACATTC
    CAATTAGATC AAATCTCTGG ATCTGGATCG GGATTAGGCA GTGGGTCAGG GTACAGCTCT
    GAAGAAAGTG GATCTACCTC AGACCTTTCC AGCAGCTCAG GAGAGAGTGG TGAGAGTGGA
    GATCTTTCTG GCATCTCCTC AGGATCAGGG TTCAGTGAGG AGTCCTCAGG ATTCAGTGGT
    TTTGCATCAG GTATTTTTCC CTCTGGTGGA TCCAGTGACA TTTCATTCAT TGATGGCAGT
    GGAATCATCA TGGTGGACAG GCCGTGGATG AAGATATCTT CATTTTTAAC AGAACAAGAG
    CTGGGTAGTG GTCAAGTCGA TTTCAGTGGA TCAGGACTTT TCTCAGGGTC TGGGGTGAGT
    GGTGATATCT CTGGCTTAAG TGGTGAAATC TCTGGATCGA GTGGTGATAG TTCAGGTATA
    AGTAGTGACA TTTCAGGAAT GAGTGGTGAT ATCTCAGGAA TGAGTGGTGA TATCTCAGGA
    ATAAGTGGTG ATATTACAGG AATGAGTGGT GATCTCTCTG GTATGAGTAG CAGTGACCAC
    AGTGGCTCAG GGAGTGGTTT AGCATTTTCT GGGGTGACAT TTTTGGGGTC AGGCTTCACT
    GATCTCACAG AACAATCTCG TGAACAGGAA GCTTCTGGAC TTGTGTTCTT TGGGTCTGGT
    GAGGGAAGTG GATTTAGATC TGGAGGCTAT GCAATTGAAT CAGGGGAACG ATCAGGGGAC
    TCGGCTAGTA GTGATGTCAT CTTCTTCAGC AATAATGACA TGGTGGAAAT GTCAAACAAG
    CCACTTCAAG GAATGGAATT AGGAAGAGGG GAAGTGGAAT ATAGTGGCAC AGTCAGACTG
    TCCTCAGGGA GTGGAGATGA CCAAAGTGTC TTTATAAGCG GGCATGAATG GATCCCAGTC
    ACTGCTAATA CACCATCTGT AAAATCCACT GATCCTCCCC CAGCTGAATT ATTTGATCAA
    GGTCCATCAG ACCTGTACAG TGATACTGCA GGACACCTAG GTCCTTATTT AAAACCAGAA
    GAGATCATCA ACAATCTTGA CGCTGTTCCT TTGGCAGCTT TGTCTTCTAC TACAGAAACT
    GCCTCTTTAC AGAGGCCTGC TGTCGTAGAA CGACCTGCTG TGAATGATTA TCTTAACCCA
    TGTGAACCAA ACCCTTGTGG AGCTGGTGTG TGTTCAGTGA AGGATGGAGT GGGACTTTGT
    CACTGCCCAC CTGGACTGCA TGGAGAAGAG TGCCAGTTTG AGGTTGATGT ATGCCATTCA
    AACCCTTGCG CCAATGGAGC CACCTGTGTG GAGGTAGAGG ACACTTTCAA ATGCTTATGT
    CTGCCCAGCT ACGAAGGAGA CCGGTGTGAG ACTGATTCAC ACAGCTGTGA GAAGGGCTGG
    ACTAAGTTTC AGGGCAACTG TTATTTACAT TTCTCGAAGA GAGAGACCTG GCTGGATGCT
    GAGCAACGCT GTCGAGACTT AAATGCTCAC CTGGTGAGCA TTAACACTCC AGAGGAGCAA
    GCCTTCGTCA ACTCAAATGC ACAAGACTAT CAGTGGATTG GACTAAATGA TAAAACAGTG
    GAAAATGACT TCCGCTGGTC AGATGGAACC CAGCTGCAAT TTGAGAACTG GAGACCAAAC
    CAGCCTGACA ATTATTTCAA CTCTGAAGAG GATTGCGTTG TGATGATCTG GCATGAAAAT
    GGCCAGTGGA ATGACGTGCC CTGCAACTAC CATCTGCCTT TTACATGCAA ATCAGGTCCA
    GTGACATGTG ATAAACCTCC GAAAGTGGAG AATGCCAAAA TGTTTGGAAA CAAGAAAGAG
    CGTTACCAGG TTAATTCTAT TATCAGATAC CAGTGCAGTG AAAACTTTAC ACAGCGCCAC
    CCACCTGTGA TCCGCTGCAT GGCAGACGGA CAGTGGGAGA AGCCAAAAGT TCAATGCATA
    CCCAAGGTCA AATTTGGACT TCACGACAAA TCTGTTCATC ACTCTCATAA AGCTAGAACA
    GCATTGGAGA AACAACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333626.1
    CDS167..4807
    Translation

    Target ORF information:

    RefSeq Version XM_021477951.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio aggrecan a (acana), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477951.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGCTGTCCT TGCTTTTGCT GAGCATGTGT TTGTGGGTTT TCTCAGCCAC GGCCTCTTTC 
    GATTACATTG ACACTGAGCC TGAAAGCCCA CTGAGAGTCA GTATACCCAA TGAGCTTCCC
    CTCCGCCCTC TGATGGGTGA CACATTAGTT CTGCCCTGCT ACTTCCAGGA CAACACAGTC
    AACGATCCTG GCGCCCCCAC AATCGCCCCC TTGTCCCACC GGATCAAGTG GAGTCTGGTC
    ACTAAAGAGA AGACCACAAA CATTTTAGTG GCCTCCGAAG GGATAGTAAG CATTGAGAAG
    AGGTATATGG ATAGAATCAC ACTGGTAGGA TATCCCATGA CCCCCACCGA CGCCTCTATT
    AAGATCACTG AGCTCCATTC CAATGACTCT GGAGTTTACC GCTGTGAGGT CATGCAGGGG
    ATTGAGGACA GTCATGATAT CGTTGATGTC CAAGTACAAG GTATTGTTTT CCACTATCGT
    GCAATCACTA CACGATATAC TCTTAATTTT GAAAAGGCCA AGGCAGCGTG TATTCAGAAC
    AGTGCAGTGA TTGCTACACC AGAACAACTC CAAGCGGCGT ATGATGAAGG GTTTCATCAG
    TGTGATGCTG GCTGGCTTGC AGATCAGACT GTGAGGTACC CTATTCATGA TCCTCGAGAG
    GCTTGCTATG GAGACAAATA TGAATTTCCA GGAGTAAGGA CATACGGAGT GAGAGACGTG
    AATGAGACAT ATGACGTGTA CTGCTTTGCT GAGAGAATGA CAGGAAAAGT GTTTCACACA
    ACTTCCCCAA ACAAATTTAC CTTTGAGGAG GCTGAAGCTC AGTGTTCCAA GCTGGGTGCC
    AAGTTGGCCA CCACAGGACA GCTGTACCTG GCATGGAAAG GTGGTATGGA TGTTTGCAAC
    GCAGGCTGGC TGGCAGACCG AAGTGTCCGC TACCCCATCA ACATCGCCCG ACCGCAGTGT
    GGAGGAGGCC TGCTGGGAGT GCGCACCGTC TATCTGTTTC CCAATCAGAC CGGTTACCCC
    CATCCAGATT CTCGCTACGA TGCTTTCTGC TACTCTGAAA CAGGCGATGA AGGTTCAGGC
    TTTTTCCCAC TGGATCTAGA GCTGACCACA GATGGTGACA ATGGTGTCTT GACTGTGGAC
    ACAATCACAG AAAGCCATAC TGTCATTTCT AAGCCTGCCT CAACAGAAAG TGAGGTACAG
    GGAGAAGTTG TAACATATAA GCCTTCGGTG ACCACCAGTG TTGAGAACCC ATACACTGCA
    CTGGATATTT CCCACGTCCT CCCTTCGCCA CCAACCAGCT CTGTGGAAAA GGACCCTGAC
    TTTGGATCCG GTTTATCTCA ATCCAATAAG ACCGAAGATA CCATGTCTGC CACAGGTGTG
    GTCTTCCATT ACCGTGGTGG AAACTGTAGT CGTTACCCTT TTGGCTTTGT GGAGGCTCAG
    CTCGCGTGTA AGAGTATCGG CGCAGTCATT GCCAGTGCTC AGCAGCTGCA GGCGGCCTAT
    GAAGCTGGTT ATCATCAGTG TGATGCCGGC TGGCTCTCGG ATCAGACTGT CCGGTATCCC
    ATCGTGTCTC CTCGTCACCA ATGCTCTGGT GATATGGAGC AAGTTCCTGG TGTGAGGTCT
    TATGGACTGC GCCCTGCCGA TGAACGCTAT GATGTGTACT GCTATGTGGA CAAACTAAGG
    GGTCAAGTTT TCCATGCAAG TTCTTTCGAG GGATTCACGT ACGGTGAGGC AGTGGCTCAC
    TGCCAGGGCC AAAATGCTTC TCTGGCCTCC ACAGCACACC TGTATGCAGC TTGGAAGCAA
    GGCTTTGATA AGTGTCGAGC TGGCTGGCTT CTTGACCGCA GTGTGCGTTA CTCCATCAAT
    ATCCCCCGGC AGCAGTGTGG TGGAGGGAGG ACTGGAGTTC ACACAGTTTA CTTGTTTCCC
    AACCAAACCG GCTCTCCTGA CCTGCACTCC AAATTCGATG CATATTGCTT CAAGGAGGAT
    CTGGATCTTC CCCTGAATGA AAGTGAATCG CAAGAGACAG TGACGGAGGA AAAGATAGTG
    AGCATTTCAT CCCATCCTGC TCTTCACAGA CCTGGTACCC CTATCATTGC TCCCAGTCCT
    ACAGAACCTT CTGGAACTGA GGAATCTGTG GCCAGCAGTT CTGGCGATCT GTTGAGCAGT
    GGCAGTGGGG ATCAGCCAAG TGGGTCATCC GTTTCAAGTG GTGACTCCTC CGGGACAAGT
    AGCACTGATG ATCTTTTGAC AAGTGGGAGC GGGGATCAGA GTGGAAGTGG CTCATCTGTT
    ACAAGTGGAG ACTATTCTGG AGCCAGTGGC ACAGACAGTC TGTTCAGTAG TGGCAGTGGC
    GATCGGTCGA GTGGGTCATT CAGTTCCAGT GGAGACTTCT CTGGAGCCAG TGGGACTGAT
    GATTTGTTGG GCAGTGGGAG TGGGGAACAG CCAAGTGGGT CATTTGTGTC AAGCGGAGAC
    TTCTCTGGGG CCAGTGGAAG TGGGGATATG TCAAGTGGTT CTGGAAGCTC TTCAGGATCT
    GGTAGTGGTT TAGATATCTT AGTGACTTTT TCAGGGATTG ACACAGTTTT TTCAGGAGTT
    GGATCAACTT CAGGGCAACT TGAGGAGGCT GGGGAGGGAA GCACTGAGAT TCTTACATTC
    CAATTAGATC AAATCTCTGG ATCTGGATCG GGATTAGGCA GTGGGTCAGG GTACAGCTCT
    GAAGAAAGTG GATCTACCTC AGACCTTTCC AGCAGCTCAG GAGAGAGTGG TGAGAGTGGA
    GATCTTTCTG GCATCTCCTC AGGATCAGGG TTCAGTGAGG AGTCCTCAGG ATTCAGTGGT
    TTTGCATCAG GTATTTTTCC CTCTGGTGGA TCCAGTGACA TTTCATTCAT TGATGGCAGT
    GGAATCATCA TGGTGGACAG GCCGTGGATG AAGATATCTT CATTTTTAAC AGAACAAGAG
    CTGGGTAGTG GTCAAGTCGA TTTCAGTGGA TCAGGACTTT TCTCAGGGTC TGGGGTGAGT
    GGTGATATCT CTGGCTTAAG TGGTGAAATC TCTGGATCGA GTGGTGATAG TTCAGGTATA
    AGTAGTGACA TTTCAGGAAT GAGTGGTGAT ATCTCAGGAA TGAGTGGTGA TATCTCAGGA
    ATAAGTGGTG ATATTACAGG AATGAGTGGT GATCTCTCTG GTATGAGTAG CAGTGACCAC
    AGTGGCTCAG GGAGTGGTTT AGCATTTTCT GGGGTGACAT TTTTGGGGTC AGGCTTCACT
    GATCTCACAG AACAATCTCG TGAACAGGAA GCTTCTGGAC TTGTGTTCTT TGGGTCTGGT
    GAGGGAAGTG GATTTAGATC TGGAGGCTAT GCAATTGAAT CAGGGGAACG ATCAGGGGAC
    TCGGCTAGTA GTGATGTCAT CTTCTTCAGC AATAATGACA TGGTGGAAAT GTCAAACAAG
    CCACTTCAAG GAATGGAATT AGGAAGAGGG GAAGTGGAAT ATAGTGGCAC AGTCAGACTG
    TCCTCAGGGA GTGGAGATGA CCAAAGTGTC TTTATAAGCG GGCATGAATG GATCCCAGTC
    ACTGCTAATA CACCATCTGT AAAATCCACT GATCCTCCCC CAGCTGAATT ATTTGATCAA
    GGTCCATCAG ACCTGTACAG TGATACTGCA GGACACCTAG GTCCTTATTT AAAACCAGAA
    GAGATCATCA ACAATCTTGA CGCTGTTCCT TTGGCAGCTT TGTCTTCTAC TACAGAAACT
    GCCTCTTTAC AGAGGCCTGC TGTCGTAGAA CGACCTGCTG TGAATGATTA TCTTAACCCA
    TGTGAACCAA ACCCTTGTGG AGCTGGTGTG TGTTCAGTGA AGGATGGAGT GGGACTTTGT
    CACTGCCCAC CTGGACTGCA TGGAGAAGAG TGCCAGTTTG AGGTTGATGT ATGCCATTCA
    AACCCTTGCG CCAATGGAGC CACCTGTGTG GAGGTAGAGG ACACTTTCAA ATGCTTATGT
    CTGCCCAGCT ACGAAGGAGA CCGGTGTGAG ACTGATTCAC ACAGCTGTGA GAAGGGCTGG
    ACTAAGTTTC AGGGCAACTG TTATTTACAT TTCTCGAAGA GAGAGACCTG GCTGGATGCT
    GAGCAACGCT GTCGAGACTT AAATGCTCAC CTGGTGAGCA TTAACACTCC AGAGGAGCAA
    GCCTTCGTCA ACTCAAATGC ACAAGACTAT CAGTGGATTG GACTAAATGA TAAAACAGTG
    GAAAATGACT TCCGCTGGTC AGATGGAACC CAGCTGCAAT TTGAGAACTG GAGACCAAAC
    CAGCCTGACA ATTATTTCAA CTCTGAAGAG GATTGCGTTG TGATGATCTG GCATGAAAAT
    GGCCAGTGGA ATGACGTGCC CTGCAACTAC CATCTGCCTT TTACATGCAA ATCAGGTCCA
    GTGACATGTG ATAAACCTCC GAAAGTGGAG AATGCCAAAA TGTTTGGAAA CAAGAAAGAG
    CGTTACCAGG TTAATTCTAT TATCAGATAC CAGTGCAGTG AAAACTTTAC ACAGCGCCAC
    CCACCTGTGA TCCGCTGCAT GGCAGACGGA CAGTGGGAGA AGCCAAAAGT TCAATGCATA
    CCCAAGGTCA AATTTGGACT TCACGACAAA TCTGTTCATC ACTCTCATAA AGCTAGAACA
    GCATTGGAGA AACAACTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.