GRASP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRASP
Entrez Gene ID	160622
Full Name	general receptor for phosphoinositides 1 associated scaffold protein
Synonyms	TAMALIN
General protein information	Preferred Names general receptor for phosphoinositides 1 associated scaffold protein Names general receptor for phosphoinositides 1-associated scaffold protein GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	This gene encodes a protein that functions as a molecular scaffold, linking receptors, including group 1 metabotropic glutamate receptors, to neuronal proteins. The encoded protein contains conserved domains, including a leucine zipper sequence, PDZ domain and a C-terminal PDZ-binding motif. Alternately spliced transcript variants have been observed for this gene.[provided by RefSeq, Dec 2012].
Disorder MIM:	612027

mRNA and Protein(s)

mRNA	Protein	Name
NM_181711.3	NP_859062.1	general receptor for phosphoinositides 1-associated scaffold protein isoform 1
NM_181711.4	NP_859062.1	general receptor for phosphoinositides 1-associated scaffold protein isoform 1
NM_001271856.1	NP_001258785.1	general receptor for phosphoinositides 1-associated scaffold protein isoform 2
NM_001271856.2	NP_001258785.1	general receptor for phosphoinositides 1-associated scaffold protein isoform 2
XM_005268691.3	XP_005268748.1	general receptor for phosphoinositides 1-associated scaffold protein isoform X1
XM_011537996.1	XP_011536298.1	general receptor for phosphoinositides 1-associated scaffold protein isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRASP	XP_001141173.2
Bos taurus (cattle)	GRASP	NP_001179070.1
Rattus norvegicus (Norway rat)	Grasp	NP_620249.1
Danio rerio (zebrafish)	grasp	NP_001035013.1
Homo sapiens (human)	GRASP	NP_859062.1
	GRASP	XP_001085174.2
Mus musculus (house mouse)	Grasp	NP_062391.3
Xenopus tropicalis (tropical clawed frog)	grasp	NP_001120354.1

Gene Ontology
Bibliography

Related articles in PubMed

Metabotropic glutamate receptor 5, and its trafficking molecules Norbin and Tamalin, are increased in the CA1 hippocampal region of subjects with schizophrenia.
Matosin N, Fernandez-Enright F, Lum JS, Andrews JL, Engel M, Huang XF, Newell KA
Schizophrenia research166(1-3)212-8(2015 Aug)

Genome-wide association analysis demonstrates the highly polygenic character of age-related hearing impairment.
Fransen E, Bonneux S, Corneveaux JJ, Schrauwen I, Di Berardino F, White CH, Ohmen JD, Van de Heyning P, Ambrosetti U, Huentelman MJ, Van Camp G, Friedman RA
European journal of human genetics : EJHG23(1)110-5(2015 Jan)

Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering.
Heinrich F, Nanda H, Goh HZ, Bachert C, L?sche M, Linstedt AD
The Journal of biological chemistry289(14)9683-91(2014 Apr)

The scaffolding protein GRASP/Tamalin directly binds to Dock180 as well as to cytohesins facilitating GTPase crosstalk in epithelial cell migration.
Attar MA, Santy LC
BMC cell biology149(2013 Feb)

Grp1-associated scaffold protein (GRASP) is a regulator of the ADP ribosylation factor 6 (Arf6)-dependent membrane trafficking pathway.
Venkataraman A, Nevrivy DJ, Filtz TM, Leid M
Cell biology international36(12)1115-28(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Schizophrenia subjects compared to controls showed a marked increase in CA1 hippocampal Norbin, and Tamalin proteins (47% and 34% respectively), which are endogenous regulators of mGluR5 signalling and trafficking
Title: Metabotropic glutamate receptor 5, and its trafficking molecules Norbin and Tamalin, are increased in the CA1 hippocampal region of subjects with schizophrenia.

myristoylated GRASP promoted tethering and exhibited a unique membrane complex. Thus, myristoylation restricts the membrane orientation of the GRASP domain favoring interactions in trans for membrane tethering.
Title: Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering.

GRASP bridges the guanine nucleotide exchange factors (GEFs) that activate Arf and Rac, thereby promoting Arf-to-Rac signaling By binding to both cytohesin 2/ARNO and Dock180.
Title: The scaffolding protein GRASP/Tamalin directly binds to Dock180 as well as to cytohesins facilitating GTPase crosstalk in epithelial cell migration.

the structural basis of the phosphoinhibition of GRASP-mediated membrane tethering and provide a mechanism for its allosteric regulation.
Title: Allosteric regulation of GRASP protein-dependent Golgi membrane tethering by mitotic phosphorylation.

GRASP regulates the non-clathrin/Arf6-dependent, plasma membrane recycling and signalling pathways.
Title: Grp1-associated scaffold protein (GRASP) is a regulator of the ADP ribosylation factor 6 (Arf6)-dependent membrane trafficking pathway.

The following GRASP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRASP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03973	NM_001271856.2 Latest version!	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu00669	NM_181711.4 Latest version!	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu46358	XM_011537996.1 Latest version!	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu46358	XM_005268691.3 Latest version!	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
View Old Accession Versions >>
OHu00669	NM_181711.3	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
OHu03973	NM_001271856.1	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRASP ( NM_001271856.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258785.1 Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03973
Clone ID Related Accession (Same CDS sequence)	NM_001271856.1 , NM_001271856.2
Accession Version	NM_001271856.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Homo sapiens(human)
Product	general receptor for phosphoinositides 1-associated scaffold protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY035281.1, BC035500.2 and BC052967.1. Transcript Variant: This variant (2) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035500.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTTAC TCCCAAGCAA AGGGGGGGAC ACCATCGCCA GCGTCAATGG CCTGAATGTG 
GAAGGCATCC GGCATCGAGA GATTGTGGAC ATCATTAAGG CGTCAGGCAA TGTTCTCAGA 
CTGGAAACTC TATATGGGAC ATCAATTCGG AAGGCAGAAC TGGAGGCTCG TCTGCAGTAC 
CTGAAGCAAA CCCTGTATGA GAAGTGGGGA GAGTACAGGT CCCTAATGGT GCAGGAGCAG 
CGGCTGGTGC ATGGCCTGGT GGTGAAGGAC CCCAGCATCT ACGACACGCT GGAGTCGGTG 
CGCTCCTGCC TCTACGGCGC GGGCCTGCTC CCGGGCTCGC TGCCCTTCGG GCCTCTGCTC 
GCCGTGCCCG GGCGTCCCCG CGGAGGCGCC CGACGGGCCA GGGGCGACGC CGACGACGCC 
GTCTACCACA CGTGCTTCTT CGGGGACTCC GAGCCGCCGG CGCTGCCGCC CCCGCCGCCC 
CCGGCCCGCG CCTTCGGCCC GGGCCCCGCC GAGACCCCTG CCGTGGGGCC GGGCCCTGGG 
CCGCGGGCCG CGCTGAGCCG CAGCGCCAGT GTGCGGTGCG CGGGCCCTGG CGGGGGCGGA 
GGCGGGGGCG CGCCGGGCGC GCTCTGGACT GAGGCTCGCG AGCAGGCCCT ATGCGGCCCC 
GGCCTGCGCA AAACCAAGTA CCGCAGCTTC CGCCGGCGGC TGCTCAAGTT CATCCCCGGA 
CTCAACCGCT CCCTGGAGGA GGAGGAGAGC CAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258785.1
CDS	271..1029
Translation	MTLLPSKGGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	NM_001271856.1
Organism	Homo sapiens(human)
Definition	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271856.1

ORF Insert Sequence:

ATGACCTTAC TCCCAAGCAA AGGGGGGGAC ACCATCGCCA GCGTCAATGG CCTGAATGTG 
GAAGGCATCC GGCATCGAGA GATTGTGGAC ATCATTAAGG CGTCAGGCAA TGTTCTCAGA 
CTGGAAACTC TATATGGGAC ATCAATTCGG AAGGCAGAAC TGGAGGCTCG TCTGCAGTAC 
CTGAAGCAAA CCCTGTATGA GAAGTGGGGA GAGTACAGGT CCCTAATGGT GCAGGAGCAG 
CGGCTGGTGC ATGGCCTGGT GGTGAAGGAC CCCAGCATCT ACGACACGCT GGAGTCGGTG 
CGCTCCTGCC TCTACGGCGC GGGCCTGCTC CCGGGCTCGC TGCCCTTCGG GCCTCTGCTC 
GCCGTGCCCG GGCGTCCCCG CGGAGGCGCC CGACGGGCCA GGGGCGACGC CGACGACGCC 
GTCTACCACA CGTGCTTCTT CGGGGACTCC GAGCCGCCGG CGCTGCCGCC CCCGCCGCCC 
CCGGCCCGCG CCTTCGGCCC GGGCCCCGCC GAGACCCCTG CCGTGGGGCC GGGCCCTGGG 
CCGCGGGCCG CGCTGAGCCG CAGCGCCAGT GTGCGGTGCG CGGGCCCTGG CGGGGGCGGA 
GGCGGGGGCG CGCCGGGCGC GCTCTGGACT GAGGCTCGCG AGCAGGCCCT ATGCGGCCCC 
GGCCTGCGCA AAACCAAGTA CCGCAGCTTC CGCCGGCGGC TGCTCAAGTT CATCCCCGGA 
CTCAACCGCT CCCTGGAGGA GGAGGAGAGC CAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRASP ( NM_001271856.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001258785.1 Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03973
Clone ID Related Accession (Same CDS sequence)	NM_001271856.1 , NM_001271856.2
Accession Version	NM_001271856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	general receptor for phosphoinositides 1-associated scaffold protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY035281.1, BC035500.2 and BC052967.1. On May 31, 2019 this sequence version replaced NM_001271856.1. Transcript Variant: This variant (2) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon, compared to variant 1. The encoded protein (isoform 2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC035500.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2146236 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCTTAC TCCCAAGCAA AGGGGGGGAC ACCATCGCCA GCGTCAATGG CCTGAATGTG 
GAAGGCATCC GGCATCGAGA GATTGTGGAC ATCATTAAGG CGTCAGGCAA TGTTCTCAGA 
CTGGAAACTC TATATGGGAC ATCAATTCGG AAGGCAGAAC TGGAGGCTCG TCTGCAGTAC 
CTGAAGCAAA CCCTGTATGA GAAGTGGGGA GAGTACAGGT CCCTAATGGT GCAGGAGCAG 
CGGCTGGTGC ATGGCCTGGT GGTGAAGGAC CCCAGCATCT ACGACACGCT GGAGTCGGTG 
CGCTCCTGCC TCTACGGCGC GGGCCTGCTC CCGGGCTCGC TGCCCTTCGG GCCTCTGCTC 
GCCGTGCCCG GGCGTCCCCG CGGAGGCGCC CGACGGGCCA GGGGCGACGC CGACGACGCC 
GTCTACCACA CGTGCTTCTT CGGGGACTCC GAGCCGCCGG CGCTGCCGCC CCCGCCGCCC 
CCGGCCCGCG CCTTCGGCCC GGGCCCCGCC GAGACCCCTG CCGTGGGGCC GGGCCCTGGG 
CCGCGGGCCG CGCTGAGCCG CAGCGCCAGT GTGCGGTGCG CGGGCCCTGG CGGGGGCGGA 
GGCGGGGGCG CGCCGGGCGC GCTCTGGACT GAGGCTCGCG AGCAGGCCCT ATGCGGCCCC 
GGCCTGCGCA AAACCAAGTA CCGCAGCTTC CGCCGGCGGC TGCTCAAGTT CATCCCCGGA 
CTCAACCGCT CCCTGGAGGA GGAGGAGAGC CAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258785.1
CDS	271..1029
Translation	MTLLPSKGGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	NM_001271856.2
Organism	Homo sapiens(human)
Definition	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271856.2

ORF Insert Sequence:

ATGACCTTAC TCCCAAGCAA AGGGGGGGAC ACCATCGCCA GCGTCAATGG CCTGAATGTG 
GAAGGCATCC GGCATCGAGA GATTGTGGAC ATCATTAAGG CGTCAGGCAA TGTTCTCAGA 
CTGGAAACTC TATATGGGAC ATCAATTCGG AAGGCAGAAC TGGAGGCTCG TCTGCAGTAC 
CTGAAGCAAA CCCTGTATGA GAAGTGGGGA GAGTACAGGT CCCTAATGGT GCAGGAGCAG 
CGGCTGGTGC ATGGCCTGGT GGTGAAGGAC CCCAGCATCT ACGACACGCT GGAGTCGGTG 
CGCTCCTGCC TCTACGGCGC GGGCCTGCTC CCGGGCTCGC TGCCCTTCGG GCCTCTGCTC 
GCCGTGCCCG GGCGTCCCCG CGGAGGCGCC CGACGGGCCA GGGGCGACGC CGACGACGCC 
GTCTACCACA CGTGCTTCTT CGGGGACTCC GAGCCGCCGG CGCTGCCGCC CCCGCCGCCC 
CCGGCCCGCG CCTTCGGCCC GGGCCCCGCC GAGACCCCTG CCGTGGGGCC GGGCCCTGGG 
CCGCGGGCCG CGCTGAGCCG CAGCGCCAGT GTGCGGTGCG CGGGCCCTGG CGGGGGCGGA 
GGCGGGGGCG CGCCGGGCGC GCTCTGGACT GAGGCTCGCG AGCAGGCCCT ATGCGGCCCC 
GGCCTGCGCA AAACCAAGTA CCGCAGCTTC CGCCGGCGGC TGCTCAAGTT CATCCCCGGA 
CTCAACCGCT CCCTGGAGGA GGAGGAGAGC CAGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRASP ( NM_181711.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_859062.1 Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00669
Clone ID Related Accession (Same CDS sequence)	NM_181711.3 , NM_181711.4
Accession Version	NM_181711.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	general receptor for phosphoinositides 1-associated scaffold protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY052304.1, BC053666.1 and BC052967.1. This sequence is a reference standard in the RefSeqGene project. On Dec 5, 2012 this sequence version replaced NM_181711.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279868.3230.1, BC053666.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCCTCC GCCGACTCAG GAAGCTGCAG CAGAAGGAGG AGGCGGCGGC CACCCCGGAC 
CCCGCCGCCC GGACTCCCGA CTCGGAAGTC GCGCCCGCCG CTCCGGTCCC GACCCCGGGA 
CCCCCTGCCG CAGCCGCCAC CCCTGGGCCC CCAGCGGACG AGCTGTACGC GGCGCTGGAG 
GACTATCACC CTGCCGAGCT GTACCGCGCG CTCGCCGTGT CCGGGGGCAC CCTGCCCCGC 
CGAAAGGGCT CAGGATTCCG CTGGAAGAAT CTCAGCCAGA GTCCTGAACA GCAGCGGAAA 
GTGCTGACGT TGGAGAAGGA GGATAACCAG ACCTTCGGCT TTGAGATCCA GACTTATGGC 
CTTCACCACC GGGAGGAGCA GCGTGTGGAA ATGGTGACCT TTGTCTGCCG AGTTCATGAG 
TCTAGCCCTG CCCAGCTGGC TGGGCTCACA CCAGGGGACA CCATCGCCAG CGTCAATGGC 
CTGAATGTGG AAGGCATCCG GCATCGAGAG ATTGTGGACA TCATTAAGGC GTCAGGCAAT 
GTTCTCAGAC TGGAAACTCT ATATGGGACA TCAATTCGGA AGGCAGAACT GGAGGCTCGT 
CTGCAGTACC TGAAGCAAAC CCTGTATGAG AAGTGGGGAG AGTACAGGTC CCTAATGGTG 
CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG GTGAAGGACC CCAGCATCTA CGACACGCTG 
GAGTCGGTGC GCTCCTGCCT CTACGGCGCG GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG 
CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC GGAGGCGCCC GACGGGCCAG GGGCGACGCC 
GACGACGCCG TCTACCACAC GTGCTTCTTC GGGGACTCCG AGCCGCCGGC GCTGCCGCCC 
CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG GGCCCCGCCG AGACCCCTGC CGTGGGGCCG 
GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC 
GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG CTCTGGACTG AGGCTCGCGA GCAGGCCCTA 
TGCGGCCCCG GCCTGCGCAA AACCAAGTAC CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC 
ATCCCCGGAC TCAACCGCTC CCTGGAGGAG GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_859062.1
CDS	76..1263
Translation	MTLRRLRKLQQKEEAAATPDPAARTPDSEVAPAAPVPTPGPPAAAATPGPPADELYAALEDYHPAELYRALAVSGGTLPRRKGSGFRWKNLSQSPEQQRKVLTLEKEDNQTFGFEIQTYGLHHREEQRVEMVTFVCRVHESSPAQLAGLTPGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	NM_181711.3
Organism	Homo sapiens(human)
Definition	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181711.3

ORF Insert Sequence:

ATGACCCTCC GCCGACTCAG GAAGCTGCAG CAGAAGGAGG AGGCGGCGGC CACCCCGGAC 
CCCGCCGCCC GGACTCCCGA CTCGGAAGTC GCGCCCGCCG CTCCGGTCCC GACCCCGGGA 
CCCCCTGCCG CAGCCGCCAC CCCTGGGCCC CCAGCGGACG AGCTGTACGC GGCGCTGGAG 
GACTATCACC CTGCCGAGCT GTACCGCGCG CTCGCCGTGT CCGGGGGCAC CCTGCCCCGC 
CGAAAGGGCT CAGGATTCCG CTGGAAGAAT CTCAGCCAGA GTCCTGAACA GCAGCGGAAA 
GTGCTGACGT TGGAGAAGGA GGATAACCAG ACCTTCGGCT TTGAGATCCA GACTTATGGC 
CTTCACCACC GGGAGGAGCA GCGTGTGGAA ATGGTGACCT TTGTCTGCCG AGTTCATGAG 
TCTAGCCCTG CCCAGCTGGC TGGGCTCACA CCAGGGGACA CCATCGCCAG CGTCAATGGC 
CTGAATGTGG AAGGCATCCG GCATCGAGAG ATTGTGGACA TCATTAAGGC GTCAGGCAAT 
GTTCTCAGAC TGGAAACTCT ATATGGGACA TCAATTCGGA AGGCAGAACT GGAGGCTCGT 
CTGCAGTACC TGAAGCAAAC CCTGTATGAG AAGTGGGGAG AGTACAGGTC CCTAATGGTG 
CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG GTGAAGGACC CCAGCATCTA CGACACGCTG 
GAGTCGGTGC GCTCCTGCCT CTACGGCGCG GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG 
CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC GGAGGCGCCC GACGGGCCAG GGGCGACGCC 
GACGACGCCG TCTACCACAC GTGCTTCTTC GGGGACTCCG AGCCGCCGGC GCTGCCGCCC 
CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG GGCCCCGCCG AGACCCCTGC CGTGGGGCCG 
GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC 
GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG CTCTGGACTG AGGCTCGCGA GCAGGCCCTA 
TGCGGCCCCG GCCTGCGCAA AACCAAGTAC CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC 
ATCCCCGGAC TCAACCGCTC CCTGGAGGAG GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRASP ( NM_181711.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_859062.1 Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu00669
Clone ID Related Accession (Same CDS sequence)	NM_181711.3 , NM_181711.4
Accession Version	NM_181711.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	general receptor for phosphoinositides 1-associated scaffold protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY052304.1, BC053666.1 and BC052967.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_181711.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279868.3230.1, BC053666.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293662.9/ ENSP00000293662.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACCCTCC GCCGACTCAG GAAGCTGCAG CAGAAGGAGG AGGCGGCGGC CACCCCGGAC 
CCCGCCGCCC GGACTCCCGA CTCGGAAGTC GCGCCCGCCG CTCCGGTCCC GACCCCGGGA 
CCCCCTGCCG CAGCCGCCAC CCCTGGGCCC CCAGCGGACG AGCTGTACGC GGCGCTGGAG 
GACTATCACC CTGCCGAGCT GTACCGCGCG CTCGCCGTGT CCGGGGGCAC CCTGCCCCGC 
CGAAAGGGCT CAGGATTCCG CTGGAAGAAT CTCAGCCAGA GTCCTGAACA GCAGCGGAAA 
GTGCTGACGT TGGAGAAGGA GGATAACCAG ACCTTCGGCT TTGAGATCCA GACTTATGGC 
CTTCACCACC GGGAGGAGCA GCGTGTGGAA ATGGTGACCT TTGTCTGCCG AGTTCATGAG 
TCTAGCCCTG CCCAGCTGGC TGGGCTCACA CCAGGGGACA CCATCGCCAG CGTCAATGGC 
CTGAATGTGG AAGGCATCCG GCATCGAGAG ATTGTGGACA TCATTAAGGC GTCAGGCAAT 
GTTCTCAGAC TGGAAACTCT ATATGGGACA TCAATTCGGA AGGCAGAACT GGAGGCTCGT 
CTGCAGTACC TGAAGCAAAC CCTGTATGAG AAGTGGGGAG AGTACAGGTC CCTAATGGTG 
CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG GTGAAGGACC CCAGCATCTA CGACACGCTG 
GAGTCGGTGC GCTCCTGCCT CTACGGCGCG GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG 
CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC GGAGGCGCCC GACGGGCCAG GGGCGACGCC 
GACGACGCCG TCTACCACAC GTGCTTCTTC GGGGACTCCG AGCCGCCGGC GCTGCCGCCC 
CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG GGCCCCGCCG AGACCCCTGC CGTGGGGCCG 
GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC 
GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG CTCTGGACTG AGGCTCGCGA GCAGGCCCTA 
TGCGGCCCCG GCCTGCGCAA AACCAAGTAC CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC 
ATCCCCGGAC TCAACCGCTC CCTGGAGGAG GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_859062.1
CDS	75..1262
Translation	MTLRRLRKLQQKEEAAATPDPAARTPDSEVAPAAPVPTPGPPAAAATPGPPADELYAALEDYHPAELYRALAVSGGTLPRRKGSGFRWKNLSQSPEQQRKVLTLEKEDNQTFGFEIQTYGLHHREEQRVEMVTFVCRVHESSPAQLAGLTPGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	NM_181711.4
Organism	Homo sapiens(human)
Definition	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181711.4

ORF Insert Sequence:

ATGACCCTCC GCCGACTCAG GAAGCTGCAG CAGAAGGAGG AGGCGGCGGC CACCCCGGAC 
CCCGCCGCCC GGACTCCCGA CTCGGAAGTC GCGCCCGCCG CTCCGGTCCC GACCCCGGGA 
CCCCCTGCCG CAGCCGCCAC CCCTGGGCCC CCAGCGGACG AGCTGTACGC GGCGCTGGAG 
GACTATCACC CTGCCGAGCT GTACCGCGCG CTCGCCGTGT CCGGGGGCAC CCTGCCCCGC 
CGAAAGGGCT CAGGATTCCG CTGGAAGAAT CTCAGCCAGA GTCCTGAACA GCAGCGGAAA 
GTGCTGACGT TGGAGAAGGA GGATAACCAG ACCTTCGGCT TTGAGATCCA GACTTATGGC 
CTTCACCACC GGGAGGAGCA GCGTGTGGAA ATGGTGACCT TTGTCTGCCG AGTTCATGAG 
TCTAGCCCTG CCCAGCTGGC TGGGCTCACA CCAGGGGACA CCATCGCCAG CGTCAATGGC 
CTGAATGTGG AAGGCATCCG GCATCGAGAG ATTGTGGACA TCATTAAGGC GTCAGGCAAT 
GTTCTCAGAC TGGAAACTCT ATATGGGACA TCAATTCGGA AGGCAGAACT GGAGGCTCGT 
CTGCAGTACC TGAAGCAAAC CCTGTATGAG AAGTGGGGAG AGTACAGGTC CCTAATGGTG 
CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG GTGAAGGACC CCAGCATCTA CGACACGCTG 
GAGTCGGTGC GCTCCTGCCT CTACGGCGCG GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG 
CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC GGAGGCGCCC GACGGGCCAG GGGCGACGCC 
GACGACGCCG TCTACCACAC GTGCTTCTTC GGGGACTCCG AGCCGCCGGC GCTGCCGCCC 
CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG GGCCCCGCCG AGACCCCTGC CGTGGGGCCG 
GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC 
GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG CTCTGGACTG AGGCTCGCGA GCAGGCCCTA 
TGCGGCCCCG GCCTGCGCAA AACCAAGTAC CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC 
ATCCCCGGAC TCAACCGCTC CCTGGAGGAG GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRASP ( XM_005268691.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268748.1 Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant X1, mRNA.

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CloneID	OHu46358
Clone ID Related Accession (Same CDS sequence)	XM_005268691.3 , XM_011537996.1
Accession Version	XM_005268691.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	general receptor for phosphoinositides 1-associated scaffold protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005268691.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACCT TTGTCTGCCG AGTTCATGAG TCTAGCCCTG CCCAGCTGGC TGGGCTCACA 
CCAGGGGACA CCATCGCCAG CGTCAATGGC CTGAATGTGG AAGGCATCCG GCATCGAGAG 
ATTGTGGACA TCATTAAGGC GTCAGGCAAT GTTCTCAGAC TGGAAACTCT ATATGGGACA 
TCAATTCGGA AGGCAGAACT GGAGGCTCGT CTGCAGTACC TGAAGCAAAC CCTGTATGAG 
AAGTGGGGAG AGTACAGGTC CCTAATGGTG CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG 
GTGAAGGACC CCAGCATCTA CGACACGCTG GAGTCGGTGC GCTCCTGCCT CTACGGCGCG 
GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC 
GGAGGCGCCC GACGGGCCAG GGGCGACGCC GACGACGCCG TCTACCACAC GTGCTTCTTC 
GGGGACTCCG AGCCGCCGGC GCTGCCGCCC CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG 
GGCCCCGCCG AGACCCCTGC CGTGGGGCCG GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC 
AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG 
CTCTGGACTG AGGCTCGCGA GCAGGCCCTA TGCGGCCCCG GCCTGCGCAA AACCAAGTAC 
CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC ATCCCCGGAC TCAACCGCTC CCTGGAGGAG 
GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268748.1
CDS	1634..2431
Translation	MVTFVCRVHESSPAQLAGLTPGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	XM_005268691.3
Organism	Homo sapiens(human)
Definition	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268691.3

ORF Insert Sequence:

ATGGTGACCT TTGTCTGCCG AGTTCATGAG TCTAGCCCTG CCCAGCTGGC TGGGCTCACA 
CCAGGGGACA CCATCGCCAG CGTCAATGGC CTGAATGTGG AAGGCATCCG GCATCGAGAG 
ATTGTGGACA TCATTAAGGC GTCAGGCAAT GTTCTCAGAC TGGAAACTCT ATATGGGACA 
TCAATTCGGA AGGCAGAACT GGAGGCTCGT CTGCAGTACC TGAAGCAAAC CCTGTATGAG 
AAGTGGGGAG AGTACAGGTC CCTAATGGTG CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG 
GTGAAGGACC CCAGCATCTA CGACACGCTG GAGTCGGTGC GCTCCTGCCT CTACGGCGCG 
GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC 
GGAGGCGCCC GACGGGCCAG GGGCGACGCC GACGACGCCG TCTACCACAC GTGCTTCTTC 
GGGGACTCCG AGCCGCCGGC GCTGCCGCCC CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG 
GGCCCCGCCG AGACCCCTGC CGTGGGGCCG GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC 
AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG 
CTCTGGACTG AGGCTCGCGA GCAGGCCCTA TGCGGCCCCG GCCTGCGCAA AACCAAGTAC 
CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC ATCCCCGGAC TCAACCGCTC CCTGGAGGAG 
GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRASP ( XM_011537996.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011536298.1 Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu46358
Clone ID Related Accession (Same CDS sequence)	XM_005268691.3 , XM_011537996.1
Accession Version	XM_011537996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 798bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	general receptor for phosphoinositides 1-associated scaffold protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACCT TTGTCTGCCG AGTTCATGAG TCTAGCCCTG CCCAGCTGGC TGGGCTCACA 
CCAGGGGACA CCATCGCCAG CGTCAATGGC CTGAATGTGG AAGGCATCCG GCATCGAGAG 
ATTGTGGACA TCATTAAGGC GTCAGGCAAT GTTCTCAGAC TGGAAACTCT ATATGGGACA 
TCAATTCGGA AGGCAGAACT GGAGGCTCGT CTGCAGTACC TGAAGCAAAC CCTGTATGAG 
AAGTGGGGAG AGTACAGGTC CCTAATGGTG CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG 
GTGAAGGACC CCAGCATCTA CGACACGCTG GAGTCGGTGC GCTCCTGCCT CTACGGCGCG 
GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC 
GGAGGCGCCC GACGGGCCAG GGGCGACGCC GACGACGCCG TCTACCACAC GTGCTTCTTC 
GGGGACTCCG AGCCGCCGGC GCTGCCGCCC CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG 
GGCCCCGCCG AGACCCCTGC CGTGGGGCCG GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC 
AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG 
CTCTGGACTG AGGCTCGCGA GCAGGCCCTA TGCGGCCCCG GCCTGCGCAA AACCAAGTAC 
CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC ATCCCCGGAC TCAACCGCTC CCTGGAGGAG 
GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011536298.1
CDS	1412..2209
Translation	MVTFVCRVHESSPAQLAGLTPGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAVPGRPRGGARRARGDADDAVYHTCFFGDSEPPALPPPPPPARAFGPGPAETPAVGPGPGPRAALSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGPGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	XM_011537996.1
Organism	Homo sapiens(human)
Definition	Homo sapiens general receptor for phosphoinositides 1 associated scaffold protein (GRASP), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537996.1

ORF Insert Sequence:

ATGGTGACCT TTGTCTGCCG AGTTCATGAG TCTAGCCCTG CCCAGCTGGC TGGGCTCACA 
CCAGGGGACA CCATCGCCAG CGTCAATGGC CTGAATGTGG AAGGCATCCG GCATCGAGAG 
ATTGTGGACA TCATTAAGGC GTCAGGCAAT GTTCTCAGAC TGGAAACTCT ATATGGGACA 
TCAATTCGGA AGGCAGAACT GGAGGCTCGT CTGCAGTACC TGAAGCAAAC CCTGTATGAG 
AAGTGGGGAG AGTACAGGTC CCTAATGGTG CAGGAGCAGC GGCTGGTGCA TGGCCTGGTG 
GTGAAGGACC CCAGCATCTA CGACACGCTG GAGTCGGTGC GCTCCTGCCT CTACGGCGCG 
GGCCTGCTCC CGGGCTCGCT GCCCTTCGGG CCTCTGCTCG CCGTGCCCGG GCGTCCCCGC 
GGAGGCGCCC GACGGGCCAG GGGCGACGCC GACGACGCCG TCTACCACAC GTGCTTCTTC 
GGGGACTCCG AGCCGCCGGC GCTGCCGCCC CCGCCGCCCC CGGCCCGCGC CTTCGGCCCG 
GGCCCCGCCG AGACCCCTGC CGTGGGGCCG GGCCCTGGGC CGCGGGCCGC GCTGAGCCGC 
AGCGCCAGTG TGCGGTGCGC GGGCCCTGGC GGGGGCGGAG GCGGGGGCGC GCCGGGCGCG 
CTCTGGACTG AGGCTCGCGA GCAGGCCCTA TGCGGCCCCG GCCTGCGCAA AACCAAGTAC 
CGCAGCTTCC GCCGGCGGCT GCTCAAGTTC ATCCCCGGAC TCAACCGCTC CCTGGAGGAG 
GAGGAGAGCC AGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Metabotropic glutamate receptor 5, and its trafficking molecules Norbin and Tamalin, are increased in the CA1 hippocampal region of subjects with schizophrenia.
Schizophrenia research166(1-3)212-8(2015 Aug)
Matosin N,Fernandez-Enright F,Lum JS,Andrews JL,Engel M,Huang XF,Newell KA

Genome-wide association analysis demonstrates the highly polygenic character of age-related hearing impairment.
European journal of human genetics : EJHG23(1)110-5(2015 Jan)
Fransen E,Bonneux S,Corneveaux JJ,Schrauwen I,Di Berardino F,White CH,Ohmen JD,Van de Heyning P,Ambrosetti U,Huentelman MJ,Van Camp G,Friedman RA

Myristoylation restricts orientation of the GRASP domain on membranes and promotes membrane tethering.
The Journal of biological chemistry289(14)9683-91(2014 Apr)
Heinrich F,Nanda H,Goh HZ,Bachert C,L?sche M,Linstedt AD

The scaffolding protein GRASP/Tamalin directly binds to Dock180 as well as to cytohesins facilitating GTPase crosstalk in epithelial cell migration.
BMC cell biology149(2013 Feb)
Attar MA,Santy LC

Grp1-associated scaffold protein (GRASP) is a regulator of the ADP ribosylation factor 6 (Arf6)-dependent membrane trafficking pathway.
Cell biology international36(12)1115-28(2012)
Venkataraman A,Nevrivy DJ,Filtz TM,Leid M

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GRASP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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