Grasp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grasp
Entrez Gene ID	192254
Full Name	general receptor for phosphoinositides 1 associated scaffold protein
General protein information	Preferred Names general receptor for phosphoinositides 1 associated scaffold protein Names general receptor for phosphoinositides 1-associated scaffold protein 95 kDa postsynaptic density protein discs-large ZO-1 domain-containing protein GRP1 (general receptor for phosphoinositides 1)-associated scaffold protein GRP1-associated scaffold protein Grp1 (general receptor for phosphoinositides)-associated scaffold protein PSD-95 PDZ domain-containing protein tamalin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q36
Summary	scaffold protein; promotes intracellular trafficking and cell surface expression of group 1 metabotropic glutamate receptors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138894.1	NP_620249.1	general receptor for phosphoinositides 1-associated scaffold protein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	GRASP	XP_001141173.2
Bos taurus (cattle)	GRASP	NP_001179070.1
Rattus norvegicus (Norway rat)	Grasp	NP_620249.1
Danio rerio (zebrafish)	grasp	NP_001035013.1
Homo sapiens (human)	GRASP	NP_859062.1
	GRASP	XP_001085174.2
Mus musculus (house mouse)	Grasp	NP_062391.3
Xenopus tropicalis (tropical clawed frog)	grasp	NP_001120354.1

Gene Ontology
Bibliography

Related articles in PubMed

GRASP1 Regulates Synaptic Plasticity and Learning through Endosomal Recycling of AMPA Receptors.
Chiu SL, Diering GH, Ye B, Takamiya K, Chen CM, Jiang Y, Niranjan T, Schwartz CE, Wang T, Huganir RL
Neuron93(6)1405-1419.e8(2017 Mar)

Spinal SGK1/GRASP-1/Rab4 is involved in complete Freund's adjuvant-induced inflammatory pain via regulating dorsal horn GluR1-containing AMPA receptor trafficking in rats.
Peng HY, Chen GD, Hsieh MC, Lai CY, Huang YP, Lin TB
Pain153(12)2380-92(2012 Dec)

PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron.
Mo J, Choi S, Ahn PG, Sun W, Lee HW, Kim H
Biochemical and biophysical research communications422(2)250-5(2012 Jun)

Crystal structures of autoinhibitory PDZ domain of Tamalin: implications for metabotropic glutamate receptor trafficking regulation.
Sugi T, Oyama T, Muto T, Nakanishi S, Morikawa K, Jingami H
The EMBO journal26(8)2192-205(2007 Apr)

Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin.
Hirose M, Kitano J, Nakajima Y, Moriyoshi K, Yanagi S, Yamamura H, Muto T, Jingami H, Nakanishi S
The Journal of biological chemistry279(31)32308-15(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our results suggest that spinal SGK1 phosphorylation, which subsequently triggers the GRASP-1/Rab4 cascade, plays a pivotal role in CFA-induced inflammatory pain by regulating GluR1-containing AMPAR recycling in the dorsal horn.
Title: Spinal SGK1/GRASP-1/Rab4 is involved in complete Freund's adjuvant-induced inflammatory pain via regulating dorsal horn GluR1-containing AMPA receptor trafficking in rats.

These results suggest that Tamalin is responsible for neuronal dendritic development via regulation of EFA6A/Arf6-mediated cytoskeleton dynamics.
Title: PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron.

tamalin is a novel signaling molecule that possesses a PDZ domain and a PDZ binding motif and mediates Syk signaling in an ITAM-based fashion
Title: Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin.

Tamalin interacts with multiple neuronal proteins and several scaffold proteins in the brain through its distinct evolutionarily-conserved protein-binding domains; and tamalin mRNA is developmentally up-regulated at the postnatal period.
Title: Tamalin is a scaffold protein that interacts with multiple neuronal proteins in distinct modes of protein-protein association.

The following Grasp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grasp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01921	NM_138894.1 Latest version!	Rattus norvegicus general receptor for phosphoinositides 1 associated scaffold protein (Grasp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grasp ( NM_138894.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_620249.1 Rattus norvegicus general receptor for phosphoinositides 1 associated scaffold protein (Grasp), mRNA.

Price & Availability↑

CloneID	ORa01921
Clone ID Related Accession (Same CDS sequence)	NM_138894.1
Accession Version	NM_138894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	general receptor for phosphoinositides 1-associated scaffold protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF374272.1. ##Evidence-Data-START## Transcript exon combination :: AF374272.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGACCCTTC GCCGACTCAG GAAGCTGCAG CAGAAGGAGG AGGCGACGGC CGCGCCGGAC 
CTCGCTGGCC GGGCTCCCGA CTCCGAAGCC GCTCGCGCTG CGCCGACCCC CTCCGGCCCC 
CCGGCTGCCG CCGCTCCGCC AGGAGCCCCC GGGGACGAGC TGTACGCAGC ATTGGAGGAC 
TATCACCCTG CGGAGCTGTA CCGCGCGCTC GCCGTGTCCG GGGGCACCCT GCCCCGCCGA 
AAGGGCTCAG GATTCCGTTG GAAGAATTTT ACTCAGAGTC CTGAACAACA ACGGAAGGTT 
CTGACTTTGG AGAAGGGAGA CAACCAGACC TTTGGCTTCG AGATCCAGAC TTATGGCCTT 
CACCACCGAG AAGAGCAGCG TGTGGAGATG GTGACCTTTG TCTGCCGAGT TCACGAGTCC 
AGCCCTGCCC AGCTGGCTGG GCTCACGCCT GGGGACACCA TCGCCAGTGT CAATGGTCTC 
AACGTGGAAG GCATCCGGCA CCGGGAGATT GTGGATATCA TCAAAGCATC TGGCAACGTT 
CTCAGACTGG AAACCCTGTA TGGGACGTCC ATTCGGAAGG CAGAACTGGA GGCACGACTG 
CAGTACCTTA AGCAAACCCT GTATGAGAAG TGGGGCGAAT ACAGGTCGCT TATGGTACAG 
GAGCAGCGGC TAGTGCACGG CCTGGTGGTG AAGGACCCAA GCATCTATGA CACACTGGAG 
TCCGTGCGCT CCTGCCTCTA CGGCGCAGGC CTGCTTCCCG GCTCGCTGCC CTTTGGGCCT 
CTGCTCGCAG CGCCTGGGGG TGCCCGCGGG GGCTCACGGC GGGCCAAAGG GGACACGGAC 
GACGCCGTGT ATCACACGTG CTTCTTTGGG GGCGCCGAGC CGCAGGCGCT GCCGCCCCCA 
CCGCCCCCCG CGCGCGCGCC AGGCCCGGGC TCCGCCGAGA CTCCGGCGTC CGTGCTGTGT 
CCCGCACCCC GTGCCACGCT CAGCCGCAGC GCCAGTGTGC GGTGCGCGGG CCCAGGCGGG 
GGCGGCGGCG GGGGCGCGCC AGGAGCCCTC TGGACTGAGG CCCGTGAGCA GGCCCTGTGC 
GGCGCCGGTC TACGTAAGAC CAAGTACCGC AGCTTCCGCA GGCGGCTGCT CAAATTCATC 
CCCGGACTCA ACCGCTCCCT GGAGGAAGAG GAGAGCCAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620249.1
CDS	61..1245
Translation	MTLRRLRKLQQKEEATAAPDLAGRAPDSEAARAAPTPSGPPAAAAPPGAPGDELYAALEDYHPAELYRALAVSGGTLPRRKGSGFRWKNFTQSPEQQRKVLTLEKGDNQTFGFEIQTYGLHHREEQRVEMVTFVCRVHESSPAQLAGLTPGDTIASVNGLNVEGIRHREIVDIIKASGNVLRLETLYGTSIRKAELEARLQYLKQTLYEKWGEYRSLMVQEQRLVHGLVVKDPSIYDTLESVRSCLYGAGLLPGSLPFGPLLAAPGGARGGSRRAKGDTDDAVYHTCFFGGAEPQALPPPPPPARAPGPGSAETPASVLCPAPRATLSRSASVRCAGPGGGGGGGAPGALWTEAREQALCGAGLRKTKYRSFRRRLLKFIPGLNRSLEEEESQL

Target ORF information:

RefSeq Version	NM_138894.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus general receptor for phosphoinositides 1 associated scaffold protein (Grasp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138894.1

ORF Insert Sequence:

ATGACCCTTC GCCGACTCAG GAAGCTGCAG CAGAAGGAGG AGGCGACGGC CGCGCCGGAC 
CTCGCTGGCC GGGCTCCCGA CTCCGAAGCC GCTCGCGCTG CGCCGACCCC CTCCGGCCCC 
CCGGCTGCCG CCGCTCCGCC AGGAGCCCCC GGGGACGAGC TGTACGCAGC ATTGGAGGAC 
TATCACCCTG CGGAGCTGTA CCGCGCGCTC GCCGTGTCCG GGGGCACCCT GCCCCGCCGA 
AAGGGCTCAG GATTCCGTTG GAAGAATTTT ACTCAGAGTC CTGAACAACA ACGGAAGGTT 
CTGACTTTGG AGAAGGGAGA CAACCAGACC TTTGGCTTCG AGATCCAGAC TTATGGCCTT 
CACCACCGAG AAGAGCAGCG TGTGGAGATG GTGACCTTTG TCTGCCGAGT TCACGAGTCC 
AGCCCTGCCC AGCTGGCTGG GCTCACGCCT GGGGACACCA TCGCCAGTGT CAATGGTCTC 
AACGTGGAAG GCATCCGGCA CCGGGAGATT GTGGATATCA TCAAAGCATC TGGCAACGTT 
CTCAGACTGG AAACCCTGTA TGGGACGTCC ATTCGGAAGG CAGAACTGGA GGCACGACTG 
CAGTACCTTA AGCAAACCCT GTATGAGAAG TGGGGCGAAT ACAGGTCGCT TATGGTACAG 
GAGCAGCGGC TAGTGCACGG CCTGGTGGTG AAGGACCCAA GCATCTATGA CACACTGGAG 
TCCGTGCGCT CCTGCCTCTA CGGCGCAGGC CTGCTTCCCG GCTCGCTGCC CTTTGGGCCT 
CTGCTCGCAG CGCCTGGGGG TGCCCGCGGG GGCTCACGGC GGGCCAAAGG GGACACGGAC 
GACGCCGTGT ATCACACGTG CTTCTTTGGG GGCGCCGAGC CGCAGGCGCT GCCGCCCCCA 
CCGCCCCCCG CGCGCGCGCC AGGCCCGGGC TCCGCCGAGA CTCCGGCGTC CGTGCTGTGT 
CCCGCACCCC GTGCCACGCT CAGCCGCAGC GCCAGTGTGC GGTGCGCGGG CCCAGGCGGG 
GGCGGCGGCG GGGGCGCGCC AGGAGCCCTC TGGACTGAGG CCCGTGAGCA GGCCCTGTGC 
GGCGCCGGTC TACGTAAGAC CAAGTACCGC AGCTTCCGCA GGCGGCTGCT CAAATTCATC 
CCCGGACTCA ACCGCTCCCT GGAGGAAGAG GAGAGCCAGC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

GRASP1 Regulates Synaptic Plasticity and Learning through Endosomal Recycling of AMPA Receptors.
Neuron93(6)1405-1419.e8(2017 Mar)
Chiu SL,Diering GH,Ye B,Takamiya K,Chen CM,Jiang Y,Niranjan T,Schwartz CE,Wang T,Huganir RL

Spinal SGK1/GRASP-1/Rab4 is involved in complete Freund's adjuvant-induced inflammatory pain via regulating dorsal horn GluR1-containing AMPA receptor trafficking in rats.
Pain153(12)2380-92(2012 Dec)
Peng HY,Chen GD,Hsieh MC,Lai CY,Huang YP,Lin TB

PDZ-scaffold protein, Tamalin promotes dendritic outgrowth and arborization in rat hippocampal neuron.
Biochemical and biophysical research communications422(2)250-5(2012 Jun)
Mo J,Choi S,Ahn PG,Sun W,Lee HW,Kim H

Crystal structures of autoinhibitory PDZ domain of Tamalin: implications for metabotropic glutamate receptor trafficking regulation.
The EMBO journal26(8)2192-205(2007 Apr)
Sugi T,Oyama T,Muto T,Nakanishi S,Morikawa K,Jingami H

Phosphorylation and recruitment of Syk by immunoreceptor tyrosine-based activation motif-based phosphorylation of tamalin.
The Journal of biological chemistry279(31)32308-15(2004 Jul)
Hirose M,Kitano J,Nakajima Y,Moriyoshi K,Yanagi S,Yamamura H,Muto T,Jingami H,Nakanishi S

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Grasp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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