GRK3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK3
Entrez Gene ID	157
Full Name	G protein-coupled receptor kinase 3
Synonyms	ADRBK2,BARK2
General protein information	Preferred Names G protein-coupled receptor kinase 3 Names beta-adrenergic receptor kinase 2 adrenergic, beta, receptor kinase 2 beta-ARK-2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.1
Summary	The beta-adrenergic receptor kinase specifically phosphorylates the agonist-occupied form of the beta-adrenergic and related G protein-coupled receptors. Overall, the beta adrenergic receptor kinase 2 has 85% amino acid similarity with beta adrenergic receptor kinase 1, with the protein kinase catalytic domain having 95% similarity. These data suggest the existence of a family of receptor kinases which may serve broadly to regulate receptor function. [provided by RefSeq, Jul 2008].
Disorder MIM:	109636

mRNA and Protein(s)

mRNA	Protein	Name
XM_011529975.2	XP_011528277.1	beta-adrenergic receptor kinase 2 isoform X2
NM_001362778.2	NP_001349707.1	beta-adrenergic receptor kinase 2 isoform 2
NM_005160.3	NP_005151.2	beta-adrenergic receptor kinase 2
NM_001362778.1	NP_001349707.1	beta-adrenergic receptor kinase 2 isoform 2
NM_005160.4	NP_005151.2	beta-adrenergic receptor kinase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADRBK2	NP_005151.2
Caenorhabditis elegans (roundworm)	grk-2	NP_497235.2
Pan troglodytes (chimpanzee)	ADRBK2	XP_003317197.1
Mus musculus (house mouse)	Adrbk2	NP_796052.2
Canis lupus familiaris (dog)	ADRBK2	XP_543453.3
Drosophila melanogaster (fruit fly)	Gprk1	NP_001163051.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012026	XP_320502.4
Xenopus tropicalis (tropical clawed frog)	adrbk2	XP_002932117.2
	ADRBK2	XP_001104308.1
Bos taurus (cattle)	ADRBK2	NP_776925.1
Rattus norvegicus (Norway rat)	Adrbk2	NP_037029.1
Gallus gallus (chicken)	ADRBK2	XP_415195.4
Danio rerio (zebrafish)	adrbk2	NP_001128197.1

Gene Ontology
Bibliography

Related articles in PubMed

High expression of GRK3 is associated with favorable prognosis in pancreatic ductal adenocarcinoma.
Liu WJ, Zhou L, Liang ZY, Zhou WX, You L, Zhang TP, Zhao YP
Pathology, research and practice(2017 Nov)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells.
Sang M, Hulsurkar M, Zhang X, Song H, Zheng D, Zhang Y, Li M, Xu J, Zhang S, Ittmann M, Li W
Oncotarget7(29)45171-45185(2016 Jul)

G Protein Coupled Receptor Kinase 3 Regulates Breast Cancer Migration, Invasion, and Metastasis.
Billard MJ, Fitzhugh DJ, Parker JS, Brozowski JM, McGinnis MW, Timoshchenko RG, Serafin DS, Lininger R, Klauber-Demore N, Sahagian G, Truong YK, Sassano MF, Serody JS, Tarrant TK
PloS one11(4)e0152856(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

effects of GRK3 modulation appear to be specific to chemokine-mediated migration behaviors without influencing tumor cell proliferation or survival
Title: G Protein Coupled Receptor Kinase 3 Regulates Breast Cancer Migration, Invasion, and Metastasis.

data are consistent with the possibility that oncogenes can induce cellular stiffness via an HDAC6-induced reorganization of the vimentin intermediate filament network
Title: GRK3 is essential for metastatic cells and promotes prostate tumor progression.

Data indicate that CXCL12-induced phosphorylation at CXCR4 S346/347 was mediated by GRK2/3.
Title: Hierarchical organization of multi-site phosphorylation at the CXCR4 C terminus.

In oral squamous cell carcinomas, malignant cells and surrounding tissue overexpress the ADRBK2 gene.
Title: Activity of ?2-adrenergic receptor in oral squamous cell carcinoma is mediated by overexpression of the ADRBK2 gene: a pilot study.

GRK3 is a negative regulator of cell growth whose expression is preferentially reduced in glioblastoma of the classical subtype as a consequence of activity in primary gliomagenic pathways.
Title: Suppression of G-protein-coupled receptor kinase 3 expression is a feature of classical GBM that is required for maximal growth.

The following GRK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23778	NM_005160.4 Latest version!	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
OHu56196	XM_011529975.2 Latest version!	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu53440	NM_001362778.2 Latest version!	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
View Old Accession Versions >>
OHu53440	NM_001362778.1	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$307.30 $439.00
OHu23778	NM_005160.3	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$344.50-$482.30 $689.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK3 ( NM_005160.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005151.2 Homo sapiens G protein-coupled receptor kinase 3 (GRK3), mRNA.

Price & Availability↑

CloneID	OHu23778
Clone ID Related Accession (Same CDS sequence)	NM_005160.3 , NM_005160.4
Accession Version	NM_005160.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC036797.1, BX387339.2, AL022329.9 and BC063545.1. On Jun 2, 2007 this sequence version replaced NM_005160.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and ESTs. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.49367.1, SRR1803611.3519.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACC TGGAGGCTGT GCTGGCCGAT GTCAGTTACC TGATGGCCAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AGGATCGTCC TGCCGGAGCC CAGTATCCGG 
AGTGTGATGC AGAAGTACCT TGCAGAGAGA AATGAAATAA CCTTTGACAA GATTTTCAAT 
CAGAAAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAG GAATATGAAA AACTTGATAA TGAGGAAGAC 
CGCCTTTGCA GAAGTCGACA AATTTATGAT GCCTACATCA TGAAGGAACT TCTTTCCTGT 
TCACATCCTT TCTCAAAGCA AGCTGTAGAA CACGTACAAA GTCATTTATC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGCCT TCGAGGTGAC 
ATTTTTCAAA AATTTATGGA AAGTGACAAG TTCACTAGAT TTTGTCAGTG GAAAAACGTT 
GAATTAAATA TCCATTTGAC CATGAATGAG TTCAGTGTGC ATAGGATTAT TGGACGAGGA 
GGATTCGGGG AAGTTTATGG TTGCAGGAAA GCAGACACTG GAAAAATGTA TGCAATGAAA 
TGCTTAGATA AGAAGAGGAT CAAAATGAAA CAAGGAGAAA CATTAGCCTT AAATGAAAGA 
ATCATGTTGT CTCTTGTCAG CACAGGAGAC TGTCCTTTCA TTGTATGTAT GACCTATGCC 
TTCCATACCC CAGATAAACT CTGCTTCATC CTGGATCTGA TGAACGGGGG CGATTTGCAC 
TACCACCTTT CACAACACGG TGTGTTCTCT GAGAAGGAGA TGCGGTTTTA TGCCACTGAA 
ATCATTCTGG GTCTGGAACA CATGCACAAT CGGTTTGTTG TCTACAGAGA TTTGAAGCCA 
GCAAATATTC TCTTGGATGA ACATGGACAC GCAAGAATAT CAGATCTTGG TCTTGCCTGC 
GATTTTTCCA AAAAGAAGCC TCATGCGAGT GTTGGCACCC ATGGGTACAT GGCTCCCGAG 
GTGCTGCAGA AGGGGACGGC CTATGACAGC AGTGCCGACT GGTTCTCCCT GGGCTGCATG 
CTTTTCAAAC TTCTGAGAGG TCACAGCCCT TTCAGACAAC ATAAAACCAA AGACAAGCAT 
GAAATTGACC GAATGACACT CACCGTGAAT GTGGAACTTC CAGACACCTT CTCTCCTGAA 
CTGAAGTCCC TTTTGGAGGG CTTGCTTCAG CGAGACGTTA GCAAGCGGCT GGGCTGTCAC 
GGAGGCGGCT CACAGGAAGT AAAAGAGCAC AGCTTTTTCA AAGGTGTTGA CTGGCAGCAT 
GTCTACTTAC AAAAGTACCC ACCACCCTTG ATTCCTCCCC GGGGAGAAGT CAATGCTGCT 
GATGCCTTTG ATATTGGCTC ATTTGATGAA GAGGATACCA AAGGGATTAA GCTACTTGAT 
TGCGACCAAG AACTCTACAA GAACTTCCCT TTGGTCATCT CTGAACGCTG GCAGCAAGAA 
GTAACGGAAA CAGTTTATGA AGCAGTAAAT GCAGACACAG ATAAAATCGA GGCCAGGAAG 
AGAGCTAAAA ATAAGCAACT TGGCCACGAA GAAGATTACG CTCTGGGGAA GGACTGTATT 
ATGCACGGGT ACATGCTGAA ACTGGGAAAC CCATTTCTGA CTCAGTGGCA GCGTCGCTAT 
TTTTACCTCT TTCCAAATAG ACTTGAATGG AGAGGAGAGG GAGAGTCCCG GCAAAATTTA 
CTGACAATGG AACAGATTCT CTCTGTGGAA GAAACTCAAA TTAAAGACAA AAAATGCATT 
TTGTTCAGAA TAAAAGGAGG GAAACAATTT GTCTTGCAAT GTGAGAGTGA TCCAGAGTTT 
GTGCAGTGGA AGAAAGAGTT GAACGAAACC TTCAAGGAGG CCCAGCGGCT ATTGCGTCGT 
GCCCCGAAGT TCCTCAACAA ACCTCGGTCA GGTACTGTGG AGCTCCCAAA GCCATCCCTC 
TGTCACAGAA ACAGCAACGG CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005151.2
CDS	148..2214
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Target ORF information:

RefSeq Version	NM_005160.3
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005160.3

ORF Insert Sequence:

ATGGCGGACC TGGAGGCTGT GCTGGCCGAT GTCAGTTACC TGATGGCCAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AGGATCGTCC TGCCGGAGCC CAGTATCCGG 
AGTGTGATGC AGAAGTACCT TGCAGAGAGA AATGAAATAA CCTTTGACAA GATTTTCAAT 
CAGAAAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAG GAATATGAAA AACTTGATAA TGAGGAAGAC 
CGCCTTTGCA GAAGTCGACA AATTTATGAT GCCTACATCA TGAAGGAACT TCTTTCCTGT 
TCACATCCTT TCTCAAAGCA AGCTGTAGAA CACGTACAAA GTCATTTATC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGCCT TCGAGGTGAC 
ATTTTTCAAA AATTTATGGA AAGTGACAAG TTCACTAGAT TTTGTCAGTG GAAAAACGTT 
GAATTAAATA TCCATTTGAC CATGAATGAG TTCAGTGTGC ATAGGATTAT TGGACGAGGA 
GGATTCGGGG AAGTTTATGG TTGCAGGAAA GCAGACACTG GAAAAATGTA TGCAATGAAA 
TGCTTAGATA AGAAGAGGAT CAAAATGAAA CAAGGAGAAA CATTAGCCTT AAATGAAAGA 
ATCATGTTGT CTCTTGTCAG CACAGGAGAC TGTCCTTTCA TTGTATGTAT GACCTATGCC 
TTCCATACCC CAGATAAACT CTGCTTCATC CTGGATCTGA TGAACGGGGG CGATTTGCAC 
TACCACCTTT CACAACACGG TGTGTTCTCT GAGAAGGAGA TGCGGTTTTA TGCCACTGAA 
ATCATTCTGG GTCTGGAACA CATGCACAAT CGGTTTGTTG TCTACAGAGA TTTGAAGCCA 
GCAAATATTC TCTTGGATGA ACATGGACAC GCAAGAATAT CAGATCTTGG TCTTGCCTGC 
GATTTTTCCA AAAAGAAGCC TCATGCGAGT GTTGGCACCC ATGGGTACAT GGCTCCCGAG 
GTGCTGCAGA AGGGGACGGC CTATGACAGC AGTGCCGACT GGTTCTCCCT GGGCTGCATG 
CTTTTCAAAC TTCTGAGAGG TCACAGCCCT TTCAGACAAC ATAAAACCAA AGACAAGCAT 
GAAATTGACC GAATGACACT CACCGTGAAT GTGGAACTTC CAGACACCTT CTCTCCTGAA 
CTGAAGTCCC TTTTGGAGGG CTTGCTTCAG CGAGACGTTA GCAAGCGGCT GGGCTGTCAC 
GGAGGCGGCT CACAGGAAGT AAAAGAGCAC AGCTTTTTCA AAGGTGTTGA CTGGCAGCAT 
GTCTACTTAC AAAAGTACCC ACCACCCTTG ATTCCTCCCC GGGGAGAAGT CAATGCTGCT 
GATGCCTTTG ATATTGGCTC ATTTGATGAA GAGGATACCA AAGGGATTAA GCTACTTGAT 
TGCGACCAAG AACTCTACAA GAACTTCCCT TTGGTCATCT CTGAACGCTG GCAGCAAGAA 
GTAACGGAAA CAGTTTATGA AGCAGTAAAT GCAGACACAG ATAAAATCGA GGCCAGGAAG 
AGAGCTAAAA ATAAGCAACT TGGCCACGAA GAAGATTACG CTCTGGGGAA GGACTGTATT 
ATGCACGGGT ACATGCTGAA ACTGGGAAAC CCATTTCTGA CTCAGTGGCA GCGTCGCTAT 
TTTTACCTCT TTCCAAATAG ACTTGAATGG AGAGGAGAGG GAGAGTCCCG GCAAAATTTA 
CTGACAATGG AACAGATTCT CTCTGTGGAA GAAACTCAAA TTAAAGACAA AAAATGCATT 
TTGTTCAGAA TAAAAGGAGG GAAACAATTT GTCTTGCAAT GTGAGAGTGA TCCAGAGTTT 
GTGCAGTGGA AGAAAGAGTT GAACGAAACC TTCAAGGAGG CCCAGCGGCT ATTGCGTCGT 
GCCCCGAAGT TCCTCAACAA ACCTCGGTCA GGTACTGTGG AGCTCCCAAA GCCATCCCTC 
TGTCACAGAA ACAGCAACGG CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( NM_005160.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005151.2 Homo sapiens G protein-coupled receptor kinase 3 (GRK3), mRNA.

Price & Availability↑

CloneID	OHu23778
Clone ID Related Accession (Same CDS sequence)	NM_005160.3 , NM_005160.4
Accession Version	NM_005160.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022329.9, BC036797.1, BX387339.2 and BC063545.1. On Nov 23, 2018 this sequence version replaced NM_005160.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and ESTs. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.49367.1, SRR5189667.339304.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000324198.11/ ENSP00000317578.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGACC TGGAGGCTGT GCTGGCCGAT GTCAGTTACC TGATGGCCAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AGGATCGTCC TGCCGGAGCC CAGTATCCGG 
AGTGTGATGC AGAAGTACCT TGCAGAGAGA AATGAAATAA CCTTTGACAA GATTTTCAAT 
CAGAAAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAG GAATATGAAA AACTTGATAA TGAGGAAGAC 
CGCCTTTGCA GAAGTCGACA AATTTATGAT GCCTACATCA TGAAGGAACT TCTTTCCTGT 
TCACATCCTT TCTCAAAGCA AGCTGTAGAA CACGTACAAA GTCATTTATC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGCCT TCGAGGTGAC 
ATTTTTCAAA AATTTATGGA AAGTGACAAG TTCACTAGAT TTTGTCAGTG GAAAAACGTT 
GAATTAAATA TCCATTTGAC CATGAATGAG TTCAGTGTGC ATAGGATTAT TGGACGAGGA 
GGATTCGGGG AAGTTTATGG TTGCAGGAAA GCAGACACTG GAAAAATGTA TGCAATGAAA 
TGCTTAGATA AGAAGAGGAT CAAAATGAAA CAAGGAGAAA CATTAGCCTT AAATGAAAGA 
ATCATGTTGT CTCTTGTCAG CACAGGAGAC TGTCCTTTCA TTGTATGTAT GACCTATGCC 
TTCCATACCC CAGATAAACT CTGCTTCATC CTGGATCTGA TGAACGGGGG CGATTTGCAC 
TACCACCTTT CACAACACGG TGTGTTCTCT GAGAAGGAGA TGCGGTTTTA TGCCACTGAA 
ATCATTCTGG GTCTGGAACA CATGCACAAT CGGTTTGTTG TCTACAGAGA TTTGAAGCCA 
GCAAATATTC TCTTGGATGA ACATGGACAC GCAAGAATAT CAGATCTTGG TCTTGCCTGC 
GATTTTTCCA AAAAGAAGCC TCATGCGAGT GTTGGCACCC ATGGGTACAT GGCTCCCGAG 
GTGCTGCAGA AGGGGACGGC CTATGACAGC AGTGCCGACT GGTTCTCCCT GGGCTGCATG 
CTTTTCAAAC TTCTGAGAGG TCACAGCCCT TTCAGACAAC ATAAAACCAA AGACAAGCAT 
GAAATTGACC GAATGACACT CACCGTGAAT GTGGAACTTC CAGACACCTT CTCTCCTGAA 
CTGAAGTCCC TTTTGGAGGG CTTGCTTCAG CGAGACGTTA GCAAGCGGCT GGGCTGTCAC 
GGAGGCGGCT CACAGGAAGT AAAAGAGCAC AGCTTTTTCA AAGGTGTTGA CTGGCAGCAT 
GTCTACTTAC AAAAGTACCC ACCACCCTTG ATTCCTCCCC GGGGAGAAGT CAATGCTGCT 
GATGCCTTTG ATATTGGCTC ATTTGATGAA GAGGATACCA AAGGGATTAA GCTACTTGAT 
TGCGACCAAG AACTCTACAA GAACTTCCCT TTGGTCATCT CTGAACGCTG GCAGCAAGAA 
GTAACGGAAA CAGTTTATGA AGCAGTAAAT GCAGACACAG ATAAAATCGA GGCCAGGAAG 
AGAGCTAAAA ATAAGCAACT TGGCCACGAA GAAGATTACG CTCTGGGGAA GGACTGTATT 
ATGCACGGGT ACATGCTGAA ACTGGGAAAC CCATTTCTGA CTCAGTGGCA GCGTCGCTAT 
TTTTACCTCT TTCCAAATAG ACTTGAATGG AGAGGAGAGG GAGAGTCCCG GCAAAATTTA 
CTGACAATGG AACAGATTCT CTCTGTGGAA GAAACTCAAA TTAAAGACAA AAAATGCATT 
TTGTTCAGAA TAAAAGGAGG GAAACAATTT GTCTTGCAAT GTGAGAGTGA TCCAGAGTTT 
GTGCAGTGGA AGAAAGAGTT GAACGAAACC TTCAAGGAGG CCCAGCGGCT ATTGCGTCGT 
GCCCCGAAGT TCCTCAACAA ACCTCGGTCA GGTACTGTGG AGCTCCCAAA GCCATCCCTC 
TGTCACAGAA ACAGCAACGG CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005151.2
CDS	367..2433
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKRIVLPEPSIRSVMQKYLAERNEITFDKIFNQKIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLDNEEDRLCRSRQIYDAYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Target ORF information:

RefSeq Version	NM_005160.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005160.4

ORF Insert Sequence:

ATGGCGGACC TGGAGGCTGT GCTGGCCGAT GTCAGTTACC TGATGGCCAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AGGATCGTCC TGCCGGAGCC CAGTATCCGG 
AGTGTGATGC AGAAGTACCT TGCAGAGAGA AATGAAATAA CCTTTGACAA GATTTTCAAT 
CAGAAAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAG GAATATGAAA AACTTGATAA TGAGGAAGAC 
CGCCTTTGCA GAAGTCGACA AATTTATGAT GCCTACATCA TGAAGGAACT TCTTTCCTGT 
TCACATCCTT TCTCAAAGCA AGCTGTAGAA CACGTACAAA GTCATTTATC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGCCT TCGAGGTGAC 
ATTTTTCAAA AATTTATGGA AAGTGACAAG TTCACTAGAT TTTGTCAGTG GAAAAACGTT 
GAATTAAATA TCCATTTGAC CATGAATGAG TTCAGTGTGC ATAGGATTAT TGGACGAGGA 
GGATTCGGGG AAGTTTATGG TTGCAGGAAA GCAGACACTG GAAAAATGTA TGCAATGAAA 
TGCTTAGATA AGAAGAGGAT CAAAATGAAA CAAGGAGAAA CATTAGCCTT AAATGAAAGA 
ATCATGTTGT CTCTTGTCAG CACAGGAGAC TGTCCTTTCA TTGTATGTAT GACCTATGCC 
TTCCATACCC CAGATAAACT CTGCTTCATC CTGGATCTGA TGAACGGGGG CGATTTGCAC 
TACCACCTTT CACAACACGG TGTGTTCTCT GAGAAGGAGA TGCGGTTTTA TGCCACTGAA 
ATCATTCTGG GTCTGGAACA CATGCACAAT CGGTTTGTTG TCTACAGAGA TTTGAAGCCA 
GCAAATATTC TCTTGGATGA ACATGGACAC GCAAGAATAT CAGATCTTGG TCTTGCCTGC 
GATTTTTCCA AAAAGAAGCC TCATGCGAGT GTTGGCACCC ATGGGTACAT GGCTCCCGAG 
GTGCTGCAGA AGGGGACGGC CTATGACAGC AGTGCCGACT GGTTCTCCCT GGGCTGCATG 
CTTTTCAAAC TTCTGAGAGG TCACAGCCCT TTCAGACAAC ATAAAACCAA AGACAAGCAT 
GAAATTGACC GAATGACACT CACCGTGAAT GTGGAACTTC CAGACACCTT CTCTCCTGAA 
CTGAAGTCCC TTTTGGAGGG CTTGCTTCAG CGAGACGTTA GCAAGCGGCT GGGCTGTCAC 
GGAGGCGGCT CACAGGAAGT AAAAGAGCAC AGCTTTTTCA AAGGTGTTGA CTGGCAGCAT 
GTCTACTTAC AAAAGTACCC ACCACCCTTG ATTCCTCCCC GGGGAGAAGT CAATGCTGCT 
GATGCCTTTG ATATTGGCTC ATTTGATGAA GAGGATACCA AAGGGATTAA GCTACTTGAT 
TGCGACCAAG AACTCTACAA GAACTTCCCT TTGGTCATCT CTGAACGCTG GCAGCAAGAA 
GTAACGGAAA CAGTTTATGA AGCAGTAAAT GCAGACACAG ATAAAATCGA GGCCAGGAAG 
AGAGCTAAAA ATAAGCAACT TGGCCACGAA GAAGATTACG CTCTGGGGAA GGACTGTATT 
ATGCACGGGT ACATGCTGAA ACTGGGAAAC CCATTTCTGA CTCAGTGGCA GCGTCGCTAT 
TTTTACCTCT TTCCAAATAG ACTTGAATGG AGAGGAGAGG GAGAGTCCCG GCAAAATTTA 
CTGACAATGG AACAGATTCT CTCTGTGGAA GAAACTCAAA TTAAAGACAA AAAATGCATT 
TTGTTCAGAA TAAAAGGAGG GAAACAATTT GTCTTGCAAT GTGAGAGTGA TCCAGAGTTT 
GTGCAGTGGA AGAAAGAGTT GAACGAAACC TTCAAGGAGG CCCAGCGGCT ATTGCGTCGT 
GCCCCGAAGT TCCTCAACAA ACCTCGGTCA GGTACTGTGG AGCTCCCAAA GCCATCCCTC 
TGTCACAGAA ACAGCAACGG CCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( XM_011529975.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011528277.1 Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant X2, mRNA.

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CloneID	OHu56196
Clone ID Related Accession (Same CDS sequence)	XM_011529975.2
Accession Version	XM_011529975.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000022.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGTG ACAAGTTCAC TAGATTTTGT CAGTGGAAAA ACGTTGAATT AAATATCCAT 
TTGACCATGA ATGAGTTCAG TGTGCATAGG ATTATTGGAC GAGGAGGATT CGGGGAAGTT 
TATGGTTGCA GGAAAGCAGA CACTGGAAAA ATGTATGCAA TGAAATGCTT AGATAAGAAG 
AGGATCAAAA TGAAACAAGG AGAAACATTA GCCTTAAATG AAAGAATCAT GTTGTCTCTT 
GTCAGCACAG GAGACTGTCC TTTCATTGTA TGTATGACCT ATGCCTTCCA TACCCCAGAT 
AAACTCTGCT TCATCCTGGA TCTGATGAAC GGGGGCGATT TGCACTACCA CCTTTCACAA 
CACGGTGTGT TCTCTGAGAA GGAGATGCGG TTTTATGCCA CTGAAATCAT TCTGGGTCTG 
GAACACATGC ACAATCGGTT TGTTGTCTAC AGAGATTTGA AGCCAGCAAA TATTCTCTTG 
GATGAACATG GACACGCAAG AATATCAGAT CTTGGTCTTG CCTGCGATTT TTCCAAAAAG 
AAGCCTCATG CGAGTGTTGG CACCCATGGG TACATGGCTC CCGAGGTGCT GCAGAAGGGG 
ACGGCCTATG ACAGCAGTGC CGACTGGTTC TCCCTGGGCT GCATGCTTTT CAAACTTCTG 
AGAGGTCACA GCCCTTTCAG ACAACATAAA ACCAAAGACA AGCATGAAAT TGACCGAATG 
ACACTCACCG TGAATGTGGA ACTTCCAGAC ACCTTCTCTC CTGAACTGAA GTCCCTTTTG 
GAGGGCTTGC TTCAGCGAGA CGTTAGCAAG CGGCTGGGCT GTCACGGAGG CGGCTCACAG 
GAAGTAAAAG AGCACAGCTT TTTCAAAGGT GTTGACTGGC AGCATGTCTA CTTACAAAAG 
TACCCACCAC CCTTGATTCC TCCCCGGGGA GAAGTCAATG CTGCTGATGC CTTTGATATT 
GGCTCATTTG ATGAAGAGGA TACCAAAGGG ATTAAGCTAC TTGATTGCGA CCAAGAACTC 
TACAAGAACT TCCCTTTGGT CATCTCTGAA CGCTGGCAGC AAGAAGTAAC GGAAACAGTT 
TATGAAGCAG TAAATGCAGA CACAGATAAA ATCGAGGCCA GGAAGAGAGC TAAAAATAAG 
CAACTTGGCC ACGAAGAAGA TTACGCTCTG GGGAAGGACT GTATTATGCA CGGGTACATG 
CTGAAACTGG GAAACCCATT TCTGACTCAG TGGCAGCGTC GCTATTTTTA CCTCTTTCCA 
AATAGACTTG AATGGAGAGG AGAGGGAGAG TCCCGGCAAA ATTTACTGAC AATGGAACAG 
ATTCTCTCTG TGGAAGAAAC TCAAATTAAA GACAAAAAAT GCATTTTGTT CAGAATAAAA 
GGAGGGAAAC AATTTGTCTT GCAATGTGAG AGTGATCCAG AGTTTGTGCA GTGGAAGAAA 
GAGTTGAACG AAACCTTCAA GGAGGCCCAG CGGCTATTGC GTCGTGCCCC GAAGTTCCTC 
AACAAACCTC GGTCAGGTAC TGTGGAGCTC CCAAAGCCAT CCCTCTGTCA CAGAAACAGC 
AACGGCCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011528277.1
CDS	353..1924
Translation	MESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Target ORF information:

RefSeq Version	XM_011529975.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529975.2

ORF Insert Sequence:

ATGGAAAGTG ACAAGTTCAC TAGATTTTGT CAGTGGAAAA ACGTTGAATT AAATATCCAT 
TTGACCATGA ATGAGTTCAG TGTGCATAGG ATTATTGGAC GAGGAGGATT CGGGGAAGTT 
TATGGTTGCA GGAAAGCAGA CACTGGAAAA ATGTATGCAA TGAAATGCTT AGATAAGAAG 
AGGATCAAAA TGAAACAAGG AGAAACATTA GCCTTAAATG AAAGAATCAT GTTGTCTCTT 
GTCAGCACAG GAGACTGTCC TTTCATTGTA TGTATGACCT ATGCCTTCCA TACCCCAGAT 
AAACTCTGCT TCATCCTGGA TCTGATGAAC GGGGGCGATT TGCACTACCA CCTTTCACAA 
CACGGTGTGT TCTCTGAGAA GGAGATGCGG TTTTATGCCA CTGAAATCAT TCTGGGTCTG 
GAACACATGC ACAATCGGTT TGTTGTCTAC AGAGATTTGA AGCCAGCAAA TATTCTCTTG 
GATGAACATG GACACGCAAG AATATCAGAT CTTGGTCTTG CCTGCGATTT TTCCAAAAAG 
AAGCCTCATG CGAGTGTTGG CACCCATGGG TACATGGCTC CCGAGGTGCT GCAGAAGGGG 
ACGGCCTATG ACAGCAGTGC CGACTGGTTC TCCCTGGGCT GCATGCTTTT CAAACTTCTG 
AGAGGTCACA GCCCTTTCAG ACAACATAAA ACCAAAGACA AGCATGAAAT TGACCGAATG 
ACACTCACCG TGAATGTGGA ACTTCCAGAC ACCTTCTCTC CTGAACTGAA GTCCCTTTTG 
GAGGGCTTGC TTCAGCGAGA CGTTAGCAAG CGGCTGGGCT GTCACGGAGG CGGCTCACAG 
GAAGTAAAAG AGCACAGCTT TTTCAAAGGT GTTGACTGGC AGCATGTCTA CTTACAAAAG 
TACCCACCAC CCTTGATTCC TCCCCGGGGA GAAGTCAATG CTGCTGATGC CTTTGATATT 
GGCTCATTTG ATGAAGAGGA TACCAAAGGG ATTAAGCTAC TTGATTGCGA CCAAGAACTC 
TACAAGAACT TCCCTTTGGT CATCTCTGAA CGCTGGCAGC AAGAAGTAAC GGAAACAGTT 
TATGAAGCAG TAAATGCAGA CACAGATAAA ATCGAGGCCA GGAAGAGAGC TAAAAATAAG 
CAACTTGGCC ACGAAGAAGA TTACGCTCTG GGGAAGGACT GTATTATGCA CGGGTACATG 
CTGAAACTGG GAAACCCATT TCTGACTCAG TGGCAGCGTC GCTATTTTTA CCTCTTTCCA 
AATAGACTTG AATGGAGAGG AGAGGGAGAG TCCCGGCAAA ATTTACTGAC AATGGAACAG 
ATTCTCTCTG TGGAAGAAAC TCAAATTAAA GACAAAAAAT GCATTTTGTT CAGAATAAAA 
GGAGGGAAAC AATTTGTCTT GCAATGTGAG AGTGATCCAG AGTTTGTGCA GTGGAAGAAA 
GAGTTGAACG AAACCTTCAA GGAGGCCCAG CGGCTATTGC GTCGTGCCCC GAAGTTCCTC 
AACAAACCTC GGTCAGGTAC TGTGGAGCTC CCAAAGCCAT CCCTCTGTCA CAGAAACAGC 
AACGGCCTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( NM_001362778.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349707.1 Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant 2, mRNA.

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CloneID	OHu53440
Clone ID Related Accession (Same CDS sequence)	NM_001362778.2 , NM_001362778.1
Accession Version	NM_001362778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022329.9 and BC029563.1. On Jun 1, 2019 this sequence version replaced NM_001362778.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.105834.1, SRR1803613.134870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAC TTCTTTCCTG TTCACATCCT TTCTCAAAGC AAGCTGTAGA ACACGTACAA 
AGTCATTTAT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGCC TTCGAGGTGA CATTTTTCAA AAATTTATGG AAAGTGACAA GTTCACTAGA 
TTTTGTCAGT GGAAAAACGT TGAATTAAAT ATCCATTTGA CCATGAATGA GTTCAGTGTG 
CATAGGATTA TTGGACGAGG AGGATTCGGG GAAGTTTATG GTTGCAGGAA AGCAGACACT 
GGAAAAATGT ATGCAATGAA ATGCTTAGAT AAGAAGAGGA TCAAAATGAA ACAAGGAGAA 
ACATTAGCCT TAAATGAAAG AATCATGTTG TCTCTTGTCA GCACAGGAGA CTGTCCTTTC 
ATTGTATGTA TGACCTATGC CTTCCATACC CCAGATAAAC TCTGCTTCAT CCTGGATCTG 
ATGAACGGGG GCGATTTGCA CTACCACCTT TCACAACACG GTGTGTTCTC TGAGAAGGAG 
ATGCGGTTTT ATGCCACTGA AATCATTCTG GGTCTGGAAC ACATGCACAA TCGGTTTGTT 
GTCTACAGAG ATTTGAAGCC AGCAAATATT CTCTTGGATG AACATGGACA CGCAAGAATA 
TCAGATCTTG GTCTTGCCTG CGATTTTTCC AAAAAGAAGC CTCATGCGAG TGTTGGCACC 
CATGGGTACA TGGCTCCCGA GGTGCTGCAG AAGGGGACGG CCTATGACAG CAGTGCCGAC 
TGGTTCTCCC TGGGCTGCAT GCTTTTCAAA CTTCTGAGAG GTCACAGCCC TTTCAGACAA 
CATAAAACCA AAGACAAGCA TGAAATTGAC CGAATGACAC TCACCGTGAA TGTGGAACTT 
CCAGACACCT TCTCTCCTGA ACTGAAGTCC CTTTTGGAGG GCTTGCTTCA GCGAGACGTT 
AGCAAGCGGC TGGGCTGTCA CGGAGGCGGC TCACAGGAAG TAAAAGAGCA CAGCTTTTTC 
AAAGGTGTTG ACTGGCAGCA TGTCTACTTA CAAAAGTACC CACCACCCTT GATTCCTCCC 
CGGGGAGAAG TCAATGCTGC TGATGCCTTT GATATTGGCT CATTTGATGA AGAGGATACC 
AAAGGGATTA AGCTACTTGA TTGCGACCAA GAACTCTACA AGAACTTCCC TTTGGTCATC 
TCTGAACGCT GGCAGCAAGA AGTAACGGAA ACAGTTTATG AAGCAGTAAA TGCAGACACA 
GATAAAATCG AGGCCAGGAA GAGAGCTAAA AATAAGCAAC TTGGCCACGA AGAAGATTAC 
GCTCTGGGGA AGGACTGTAT TATGCACGGG TACATGCTGA AACTGGGAAA CCCATTTCTG 
ACTCAGTGGC AGCGTCGCTA TTTTTACCTC TTTCCAAATA GACTTGAATG GAGAGGAGAG 
GGAGAGTCCC GGCAAAATTT ACTGACAATG GAACAGATTC TCTCTGTGGA AGAAACTCAA 
ATTAAAGACA AAAAATGCAT TTTGTTCAGA ATAAAAGGAG GGAAACAATT TGTCTTGCAA 
TGTGAGAGTG ATCCAGAGTT TGTGCAGTGG AAGAAAGAGT TGAACGAAAC CTTCAAGGAG 
GCCCAGCGGC TATTGCGTCG TGCCCCGAAG TTCCTCAACA AACCTCGGTC AGGTACTGTG 
GAGCTCCCAA AGCCATCCCT CTGTCACAGA AACAGCAACG GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349707.1
CDS	632..2359
Translation	MKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Target ORF information:

RefSeq Version	NM_001362778.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362778.2

ORF Insert Sequence:

ATGAAGGAAC TTCTTTCCTG TTCACATCCT TTCTCAAAGC AAGCTGTAGA ACACGTACAA 
AGTCATTTAT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGCC TTCGAGGTGA CATTTTTCAA AAATTTATGG AAAGTGACAA GTTCACTAGA 
TTTTGTCAGT GGAAAAACGT TGAATTAAAT ATCCATTTGA CCATGAATGA GTTCAGTGTG 
CATAGGATTA TTGGACGAGG AGGATTCGGG GAAGTTTATG GTTGCAGGAA AGCAGACACT 
GGAAAAATGT ATGCAATGAA ATGCTTAGAT AAGAAGAGGA TCAAAATGAA ACAAGGAGAA 
ACATTAGCCT TAAATGAAAG AATCATGTTG TCTCTTGTCA GCACAGGAGA CTGTCCTTTC 
ATTGTATGTA TGACCTATGC CTTCCATACC CCAGATAAAC TCTGCTTCAT CCTGGATCTG 
ATGAACGGGG GCGATTTGCA CTACCACCTT TCACAACACG GTGTGTTCTC TGAGAAGGAG 
ATGCGGTTTT ATGCCACTGA AATCATTCTG GGTCTGGAAC ACATGCACAA TCGGTTTGTT 
GTCTACAGAG ATTTGAAGCC AGCAAATATT CTCTTGGATG AACATGGACA CGCAAGAATA 
TCAGATCTTG GTCTTGCCTG CGATTTTTCC AAAAAGAAGC CTCATGCGAG TGTTGGCACC 
CATGGGTACA TGGCTCCCGA GGTGCTGCAG AAGGGGACGG CCTATGACAG CAGTGCCGAC 
TGGTTCTCCC TGGGCTGCAT GCTTTTCAAA CTTCTGAGAG GTCACAGCCC TTTCAGACAA 
CATAAAACCA AAGACAAGCA TGAAATTGAC CGAATGACAC TCACCGTGAA TGTGGAACTT 
CCAGACACCT TCTCTCCTGA ACTGAAGTCC CTTTTGGAGG GCTTGCTTCA GCGAGACGTT 
AGCAAGCGGC TGGGCTGTCA CGGAGGCGGC TCACAGGAAG TAAAAGAGCA CAGCTTTTTC 
AAAGGTGTTG ACTGGCAGCA TGTCTACTTA CAAAAGTACC CACCACCCTT GATTCCTCCC 
CGGGGAGAAG TCAATGCTGC TGATGCCTTT GATATTGGCT CATTTGATGA AGAGGATACC 
AAAGGGATTA AGCTACTTGA TTGCGACCAA GAACTCTACA AGAACTTCCC TTTGGTCATC 
TCTGAACGCT GGCAGCAAGA AGTAACGGAA ACAGTTTATG AAGCAGTAAA TGCAGACACA 
GATAAAATCG AGGCCAGGAA GAGAGCTAAA AATAAGCAAC TTGGCCACGA AGAAGATTAC 
GCTCTGGGGA AGGACTGTAT TATGCACGGG TACATGCTGA AACTGGGAAA CCCATTTCTG 
ACTCAGTGGC AGCGTCGCTA TTTTTACCTC TTTCCAAATA GACTTGAATG GAGAGGAGAG 
GGAGAGTCCC GGCAAAATTT ACTGACAATG GAACAGATTC TCTCTGTGGA AGAAACTCAA 
ATTAAAGACA AAAAATGCAT TTTGTTCAGA ATAAAAGGAG GGAAACAATT TGTCTTGCAA 
TGTGAGAGTG ATCCAGAGTT TGTGCAGTGG AAGAAAGAGT TGAACGAAAC CTTCAAGGAG 
GCCCAGCGGC TATTGCGTCG TGCCCCGAAG TTCCTCAACA AACCTCGGTC AGGTACTGTG 
GAGCTCCCAA AGCCATCCCT CTGTCACAGA AACAGCAACG GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( NM_001362778.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001349707.1 Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu53440
Clone ID Related Accession (Same CDS sequence)	NM_001362778.2 , NM_001362778.1
Accession Version	NM_001362778.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Homo sapiens(human)
Product	beta-adrenergic receptor kinase 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL022329.9 and BC029563.1. On Apr 27, 2018 this sequence version replaced XM_024452165.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.105834.1, SRR1803613.134870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGAAC TTCTTTCCTG TTCACATCCT TTCTCAAAGC AAGCTGTAGA ACACGTACAA 
AGTCATTTAT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGCC TTCGAGGTGA CATTTTTCAA AAATTTATGG AAAGTGACAA GTTCACTAGA 
TTTTGTCAGT GGAAAAACGT TGAATTAAAT ATCCATTTGA CCATGAATGA GTTCAGTGTG 
CATAGGATTA TTGGACGAGG AGGATTCGGG GAAGTTTATG GTTGCAGGAA AGCAGACACT 
GGAAAAATGT ATGCAATGAA ATGCTTAGAT AAGAAGAGGA TCAAAATGAA ACAAGGAGAA 
ACATTAGCCT TAAATGAAAG AATCATGTTG TCTCTTGTCA GCACAGGAGA CTGTCCTTTC 
ATTGTATGTA TGACCTATGC CTTCCATACC CCAGATAAAC TCTGCTTCAT CCTGGATCTG 
ATGAACGGGG GCGATTTGCA CTACCACCTT TCACAACACG GTGTGTTCTC TGAGAAGGAG 
ATGCGGTTTT ATGCCACTGA AATCATTCTG GGTCTGGAAC ACATGCACAA TCGGTTTGTT 
GTCTACAGAG ATTTGAAGCC AGCAAATATT CTCTTGGATG AACATGGACA CGCAAGAATA 
TCAGATCTTG GTCTTGCCTG CGATTTTTCC AAAAAGAAGC CTCATGCGAG TGTTGGCACC 
CATGGGTACA TGGCTCCCGA GGTGCTGCAG AAGGGGACGG CCTATGACAG CAGTGCCGAC 
TGGTTCTCCC TGGGCTGCAT GCTTTTCAAA CTTCTGAGAG GTCACAGCCC TTTCAGACAA 
CATAAAACCA AAGACAAGCA TGAAATTGAC CGAATGACAC TCACCGTGAA TGTGGAACTT 
CCAGACACCT TCTCTCCTGA ACTGAAGTCC CTTTTGGAGG GCTTGCTTCA GCGAGACGTT 
AGCAAGCGGC TGGGCTGTCA CGGAGGCGGC TCACAGGAAG TAAAAGAGCA CAGCTTTTTC 
AAAGGTGTTG ACTGGCAGCA TGTCTACTTA CAAAAGTACC CACCACCCTT GATTCCTCCC 
CGGGGAGAAG TCAATGCTGC TGATGCCTTT GATATTGGCT CATTTGATGA AGAGGATACC 
AAAGGGATTA AGCTACTTGA TTGCGACCAA GAACTCTACA AGAACTTCCC TTTGGTCATC 
TCTGAACGCT GGCAGCAAGA AGTAACGGAA ACAGTTTATG AAGCAGTAAA TGCAGACACA 
GATAAAATCG AGGCCAGGAA GAGAGCTAAA AATAAGCAAC TTGGCCACGA AGAAGATTAC 
GCTCTGGGGA AGGACTGTAT TATGCACGGG TACATGCTGA AACTGGGAAA CCCATTTCTG 
ACTCAGTGGC AGCGTCGCTA TTTTTACCTC TTTCCAAATA GACTTGAATG GAGAGGAGAG 
GGAGAGTCCC GGCAAAATTT ACTGACAATG GAACAGATTC TCTCTGTGGA AGAAACTCAA 
ATTAAAGACA AAAAATGCAT TTTGTTCAGA ATAAAAGGAG GGAAACAATT TGTCTTGCAA 
TGTGAGAGTG ATCCAGAGTT TGTGCAGTGG AAGAAAGAGT TGAACGAAAC CTTCAAGGAG 
GCCCAGCGGC TATTGCGTCG TGCCCCGAAG TTCCTCAACA AACCTCGGTC AGGTACTGTG 
GAGCTCCCAA AGCCATCCCT CTGTCACAGA AACAGCAACG GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001349707.1
CDS	413..2140
Translation	MKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGDIFQKFMESDKFTRFCQWKNVELNIHLTMNEFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHARISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTVNVELPDTFSPELKSLLEGLLQRDVSKRLGCHGGGSQEVKEHSFFKGVDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVTETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGKDCIMHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQNLLTMEQILSVEETQIKDKKCILFRIKGGKQFVLQCESDPEFVQWKKELNETFKEAQRLLRRAPKFLNKPRSGTVELPKPSLCHRNSNGL

Target ORF information:

RefSeq Version	NM_001362778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor kinase 3 (GRK3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001362778.1

ORF Insert Sequence:

ATGAAGGAAC TTCTTTCCTG TTCACATCCT TTCTCAAAGC AAGCTGTAGA ACACGTACAA 
AGTCATTTAT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGCC TTCGAGGTGA CATTTTTCAA AAATTTATGG AAAGTGACAA GTTCACTAGA 
TTTTGTCAGT GGAAAAACGT TGAATTAAAT ATCCATTTGA CCATGAATGA GTTCAGTGTG 
CATAGGATTA TTGGACGAGG AGGATTCGGG GAAGTTTATG GTTGCAGGAA AGCAGACACT 
GGAAAAATGT ATGCAATGAA ATGCTTAGAT AAGAAGAGGA TCAAAATGAA ACAAGGAGAA 
ACATTAGCCT TAAATGAAAG AATCATGTTG TCTCTTGTCA GCACAGGAGA CTGTCCTTTC 
ATTGTATGTA TGACCTATGC CTTCCATACC CCAGATAAAC TCTGCTTCAT CCTGGATCTG 
ATGAACGGGG GCGATTTGCA CTACCACCTT TCACAACACG GTGTGTTCTC TGAGAAGGAG 
ATGCGGTTTT ATGCCACTGA AATCATTCTG GGTCTGGAAC ACATGCACAA TCGGTTTGTT 
GTCTACAGAG ATTTGAAGCC AGCAAATATT CTCTTGGATG AACATGGACA CGCAAGAATA 
TCAGATCTTG GTCTTGCCTG CGATTTTTCC AAAAAGAAGC CTCATGCGAG TGTTGGCACC 
CATGGGTACA TGGCTCCCGA GGTGCTGCAG AAGGGGACGG CCTATGACAG CAGTGCCGAC 
TGGTTCTCCC TGGGCTGCAT GCTTTTCAAA CTTCTGAGAG GTCACAGCCC TTTCAGACAA 
CATAAAACCA AAGACAAGCA TGAAATTGAC CGAATGACAC TCACCGTGAA TGTGGAACTT 
CCAGACACCT TCTCTCCTGA ACTGAAGTCC CTTTTGGAGG GCTTGCTTCA GCGAGACGTT 
AGCAAGCGGC TGGGCTGTCA CGGAGGCGGC TCACAGGAAG TAAAAGAGCA CAGCTTTTTC 
AAAGGTGTTG ACTGGCAGCA TGTCTACTTA CAAAAGTACC CACCACCCTT GATTCCTCCC 
CGGGGAGAAG TCAATGCTGC TGATGCCTTT GATATTGGCT CATTTGATGA AGAGGATACC 
AAAGGGATTA AGCTACTTGA TTGCGACCAA GAACTCTACA AGAACTTCCC TTTGGTCATC 
TCTGAACGCT GGCAGCAAGA AGTAACGGAA ACAGTTTATG AAGCAGTAAA TGCAGACACA 
GATAAAATCG AGGCCAGGAA GAGAGCTAAA AATAAGCAAC TTGGCCACGA AGAAGATTAC 
GCTCTGGGGA AGGACTGTAT TATGCACGGG TACATGCTGA AACTGGGAAA CCCATTTCTG 
ACTCAGTGGC AGCGTCGCTA TTTTTACCTC TTTCCAAATA GACTTGAATG GAGAGGAGAG 
GGAGAGTCCC GGCAAAATTT ACTGACAATG GAACAGATTC TCTCTGTGGA AGAAACTCAA 
ATTAAAGACA AAAAATGCAT TTTGTTCAGA ATAAAAGGAG GGAAACAATT TGTCTTGCAA 
TGTGAGAGTG ATCCAGAGTT TGTGCAGTGG AAGAAAGAGT TGAACGAAAC CTTCAAGGAG 
GCCCAGCGGC TATTGCGTCG TGCCCCGAAG TTCCTCAACA AACCTCGGTC AGGTACTGTG 
GAGCTCCCAA AGCCATCCCT CTGTCACAGA AACAGCAACG GCCTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High expression of GRK3 is associated with favorable prognosis in pancreatic ductal adenocarcinoma.
Pathology, research and practice(2017 Nov)
Liu WJ,Zhou L,Liang ZY,Zhou WX,You L,Zhang TP,Zhao YP

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

GRK3 is a direct target of CREB activation and regulates neuroendocrine differentiation of prostate cancer cells.
Oncotarget7(29)45171-45185(2016 Jul)
Sang M,Hulsurkar M,Zhang X,Song H,Zheng D,Zhang Y,Li M,Xu J,Zhang S,Ittmann M,Li W

G Protein Coupled Receptor Kinase 3 Regulates Breast Cancer Migration, Invasion, and Metastasis.
PloS one11(4)e0152856(2016)
Billard MJ,Fitzhugh DJ,Parker JS,Brozowski JM,McGinnis MW,Timoshchenko RG,Serafin DS,Lininger R,Klauber-Demore N,Sahagian G,Truong YK,Sassano MF,Serody JS,Tarrant TK

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GRK3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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