GRK3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GRK3
Entrez Gene ID	282136
Full Name	G protein-coupled receptor kinase 3
Synonyms	ADRBK2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024977014.1	XP_024832782.1	beta-adrenergic receptor kinase 2 isoform X1
XM_024977018.1	XP_024832786.1	beta-adrenergic receptor kinase 2 isoform X3
XM_024977016.1	XP_024832784.1	beta-adrenergic receptor kinase 2 isoform X2
XM_024977017.1	XP_024832785.1	beta-adrenergic receptor kinase 2 isoform X3
NM_174500.2	NP_776925.1	beta-adrenergic receptor kinase 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ADRBK2	NP_005151.2
Caenorhabditis elegans (roundworm)	grk-2	NP_497235.2
Pan troglodytes (chimpanzee)	ADRBK2	XP_003317197.1
Mus musculus (house mouse)	Adrbk2	NP_796052.2
Canis lupus familiaris (dog)	ADRBK2	XP_543453.3
Drosophila melanogaster (fruit fly)	Gprk1	NP_001163051.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012026	XP_320502.4
Xenopus tropicalis (tropical clawed frog)	adrbk2	XP_002932117.2
	ADRBK2	XP_001104308.1
Bos taurus (cattle)	ADRBK2	NP_776925.1
Rattus norvegicus (Norway rat)	Adrbk2	NP_037029.1
Gallus gallus (chicken)	ADRBK2	XP_415195.4
Danio rerio (zebrafish)	adrbk2	NP_001128197.1

Gene Ontology
Bibliography

Related articles in PubMed

The c-terminus of GRK3 indicates rapid dissociation of G protein heterotrimers.
Hollins B, Kuravi S, Digby GJ, Lambert NA
Cellular signalling21(6)1015-21(2009 Jun)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family.
Benovic JL, Onorato JJ, Arriza JL, Stone WC, Lohse M, Jenkins NA, Gilbert DJ, Copeland NG, Caron MG, Lefkowitz RJ
The Journal of biological chemistry266(23)14939-46(1991 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fusion proteins containing the c-terminus of GPCR kinase 3 (GRK3ct) and either the fluorescent protein cerulean or Renilla luciferase bind to venus-labeled Gbetagamma dimers, resulting in Forster or bioluminescence resonance energy transfer.
Title: The c-terminus of GRK3 indicates rapid dissociation of G protein heterotrimers.

The following GRK3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GRK3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41490	NM_174500.2 Latest version!	Bos taurus G protein-coupled receptor kinase 3 (GRK3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa224149	XM_024977014.1 Latest version!	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa224150	XM_024977016.1 Latest version!	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa224151	XM_024977018.1 Latest version!	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa224151	XM_024977017.1 Latest version!	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GRK3 ( NM_174500.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776925.1 Bos taurus G protein-coupled receptor kinase 3 (GRK3), mRNA.

Price & Availability↑

CloneID	OBa41490
Clone ID Related Accession (Same CDS sequence)	NM_174500.2
Accession Version	NM_174500.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M73216.1. On Jun 3, 2003 this sequence version replaced NM_174500.1. ##Evidence-Data-START## Transcript exon combination :: M73216.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGACC TGGAGGCCGT GCTGGCCGAT GTCAGCTACC TGATGGCGAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AAGATCGTCC TGCCCGAGCC CAGTATCCGG 
AGCGTGATGC AGAAGTATCT TGAGGAGAGA CACGAAATCA CCTTTGACAA GATTTTTAAT 
CAGAGAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAA GAATATGAAA AGCTTGAGAA TGAGGAAGAT 
CGCCTTTGTA GAAGTCGACA GATTTATGAC ACTTACATCA TGAAGGAGCT GCTGTCGTGT 
TCACATCCAT TCTCAAAGCA AGCCGTAGAA CACGTACAAA GTCATCTGTC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGTCT CCGAGGCAGC 
ATTTTTCAAA AATTCATGGA AAGTGACAAG TTTACTAGAT TTTGTCAGTG GAAAAACGTG 
GAATTAAATA TCCATTTGAC CATGAATGAT TTCAGCGTGC ATCGGATCAT TGGACGAGGA 
GGATTCGGTG AAGTATACGG TTGCAGGAAA GCAGACACTG GAAAGATGTA TGCAATGAAA 
TGCTTGGATA AGAAGAGAAT CAAGATGAAA CAGGGAGAAA CCTTAGCCTT AAATGAAAGG 
ATCATGTTGT CCCTGGTGAG CACAGGAGAT TGCCCTTTCA TCGTCTGTAT GACCTATGCC 
TTCCACACTC CAGATAAACT GTGCTTCATC TTGGATCTGA TGAACGGGGG TGACCTGCAC 
TATCACCTTT CGCAGCACGG GGTGTTTTCT GAGAAGGAGA TGCGGTTTTA CGCCACAGAA 
ATCATCCTGG GGCTGGAACA CATGCACAAT CGGTTTGTTG TTTACAGAGA CTTGAAGCCC 
GCCAATATCC TCCTGGATGA GCACGGACAT GTGAGGATAT CAGACCTTGG TCTTGCCTGC 
GATTTTTCCA AAAAGAAGCC GCACGCGAGC GTGGGCACCC ACGGGTACAT GGCGCCCGAA 
GTTCTGCAGA AGGGGACCGC CTACGACAGC AGTGCCGACT GGTTCTCCCT GGGCTGTATG 
CTTTTCAAAC TTCTGAGAGG TCACAGCCCT TTCAGACAAC ATAAAACCAA AGATAAGCAT 
GAGATAGACC GAATGACTCT CACCATGAAC GTGGAACTTC CAGACGTCTT CTCCCCTGAG 
CTCAAGTCCC TTCTGGAAGG CCTGCTTCAG CGAGATGTCA GTAAGCGCCT CGGCTGCCAT 
GGAGGCAGCG CACAGGAGCT AAAAACGCAC GACTTCTTCA GAGGCATCGA CTGGCAGCAC 
GTCTACCTGC AGAAGTACCC TCCACCCTTG ATCCCTCCCC GAGGGGAAGT CAATGCAGCC 
GACGCCTTTG ACATCGGCTC ATTTGATGAA GAGGATACCA AAGGCATCAA GCTTCTTGAT 
TGCGACCAAG AACTCTACAA GAACTTCCCT CTGGTGATCT CTGAGCGCTG GCAGCAGGAA 
GTGGCGGAAA CAGTTTATGA AGCAGTAAAT GCAGACACGG ATAAAATCGA GGCCAGGAAG 
AGAGCTAAAA ATAAGCAGCT TGGCCACGAA GAAGATTACG CCCTGGGAAG AGACTGCATC 
GTGCACGGGT ACATGCTGAA GCTGGGGAAC CCTTTCCTGA CCCAGTGGCA GCGCCGCTAT 
TTTTACCTCT TTCCGAACAG ACTTGAGTGG AGAGGAGAAG GCGAGTCGCG ACAAAGTTTA 
CTGACAATGG AACAGATTGT GTCCGTGGAA GAAACTCAGA TTAAAGACAA AAAGTGCATT 
TTGTTGAGAA TAAAAGGAGG GAAGCAGTTC GTTTTGCAGT GTGAGAGTGA CCCAGAGTTT 
GTGCAGTGGA AGAAAGAGCT GACGGAGACA TTCATGGAGG CCCAGCGGCT GCTACGGCGA 
GCCCCCAAGT TCCTCAACAA ATCCCGCTCA GCCGTCGTGG AACTCTCAAA GCCTCCCCTC 
TGCCATAGGA ACAGCAACGG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776925.1
CDS	49..2115
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKIVLPEPSIRSVMQKYLEERHEITFDKIFNQRIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLENEEDRLCRSRQIYDTYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGSIFQKFMESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMNVELPDVFSPELKSLLEGLLQRDVSKRLGCHGGSAQELKTHDFFRGIDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVAETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGRDCIVHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQSLLTMEQIVSVEETQIKDKKCILLRIKGGKQFVLQCESDPEFVQWKKELTETFMEAQRLLRRAPKFLNKSRSAVVELSKPPLCHRNSNGL

Target ORF information:

RefSeq Version	NM_174500.2
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 3 (GRK3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174500.2

ORF Insert Sequence:

ATGGCGGACC TGGAGGCCGT GCTGGCCGAT GTCAGCTACC TGATGGCGAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AAGATCGTCC TGCCCGAGCC CAGTATCCGG 
AGCGTGATGC AGAAGTATCT TGAGGAGAGA CACGAAATCA CCTTTGACAA GATTTTTAAT 
CAGAGAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAA GAATATGAAA AGCTTGAGAA TGAGGAAGAT 
CGCCTTTGTA GAAGTCGACA GATTTATGAC ACTTACATCA TGAAGGAGCT GCTGTCGTGT 
TCACATCCAT TCTCAAAGCA AGCCGTAGAA CACGTACAAA GTCATCTGTC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGTCT CCGAGGCAGC 
ATTTTTCAAA AATTCATGGA AAGTGACAAG TTTACTAGAT TTTGTCAGTG GAAAAACGTG 
GAATTAAATA TCCATTTGAC CATGAATGAT TTCAGCGTGC ATCGGATCAT TGGACGAGGA 
GGATTCGGTG AAGTATACGG TTGCAGGAAA GCAGACACTG GAAAGATGTA TGCAATGAAA 
TGCTTGGATA AGAAGAGAAT CAAGATGAAA CAGGGAGAAA CCTTAGCCTT AAATGAAAGG 
ATCATGTTGT CCCTGGTGAG CACAGGAGAT TGCCCTTTCA TCGTCTGTAT GACCTATGCC 
TTCCACACTC CAGATAAACT GTGCTTCATC TTGGATCTGA TGAACGGGGG TGACCTGCAC 
TATCACCTTT CGCAGCACGG GGTGTTTTCT GAGAAGGAGA TGCGGTTTTA CGCCACAGAA 
ATCATCCTGG GGCTGGAACA CATGCACAAT CGGTTTGTTG TTTACAGAGA CTTGAAGCCC 
GCCAATATCC TCCTGGATGA GCACGGACAT GTGAGGATAT CAGACCTTGG TCTTGCCTGC 
GATTTTTCCA AAAAGAAGCC GCACGCGAGC GTGGGCACCC ACGGGTACAT GGCGCCCGAA 
GTTCTGCAGA AGGGGACCGC CTACGACAGC AGTGCCGACT GGTTCTCCCT GGGCTGTATG 
CTTTTCAAAC TTCTGAGAGG TCACAGCCCT TTCAGACAAC ATAAAACCAA AGATAAGCAT 
GAGATAGACC GAATGACTCT CACCATGAAC GTGGAACTTC CAGACGTCTT CTCCCCTGAG 
CTCAAGTCCC TTCTGGAAGG CCTGCTTCAG CGAGATGTCA GTAAGCGCCT CGGCTGCCAT 
GGAGGCAGCG CACAGGAGCT AAAAACGCAC GACTTCTTCA GAGGCATCGA CTGGCAGCAC 
GTCTACCTGC AGAAGTACCC TCCACCCTTG ATCCCTCCCC GAGGGGAAGT CAATGCAGCC 
GACGCCTTTG ACATCGGCTC ATTTGATGAA GAGGATACCA AAGGCATCAA GCTTCTTGAT 
TGCGACCAAG AACTCTACAA GAACTTCCCT CTGGTGATCT CTGAGCGCTG GCAGCAGGAA 
GTGGCGGAAA CAGTTTATGA AGCAGTAAAT GCAGACACGG ATAAAATCGA GGCCAGGAAG 
AGAGCTAAAA ATAAGCAGCT TGGCCACGAA GAAGATTACG CCCTGGGAAG AGACTGCATC 
GTGCACGGGT ACATGCTGAA GCTGGGGAAC CCTTTCCTGA CCCAGTGGCA GCGCCGCTAT 
TTTTACCTCT TTCCGAACAG ACTTGAGTGG AGAGGAGAAG GCGAGTCGCG ACAAAGTTTA 
CTGACAATGG AACAGATTGT GTCCGTGGAA GAAACTCAGA TTAAAGACAA AAAGTGCATT 
TTGTTGAGAA TAAAAGGAGG GAAGCAGTTC GTTTTGCAGT GTGAGAGTGA CCCAGAGTTT 
GTGCAGTGGA AGAAAGAGCT GACGGAGACA TTCATGGAGG CCCAGCGGCT GCTACGGCGA 
GCCCCCAAGT TCCTCAACAA ATCCCGCTCA GCCGTCGTGG AACTCTCAAA GCCTCCCCTC 
TGCCATAGGA ACAGCAACGG CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( XM_024977014.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832782.1 Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa224149
Clone ID Related Accession (Same CDS sequence)	XM_024977014.1
Accession Version	XM_024977014.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2163bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC TGGAGGCCGT GCTGGCCGAT GTCAGCTACC TGATGGCGAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AAGATCGTCC TGCCCGAGCC CAGTATCCGG 
AGCGTGATGC AGAAGTATCT TGAGGAGAGA CACGAAATCA CCTTTGACAA GATTTTTAAT 
CAGAGAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAA GAATATGAAA AGCTTGAGAA TGAGGAAGAT 
CGCCTTTGTA GAAGTCGACA GATTTATGAC ACTTACATCA TGAAGGAGCT GCTGTCGTGT 
TCACATCCAT TCTCAAAGCA AGCCGTAGAA CACGTACAAA GTCATCTGTC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGTCT CCGAGGCAGC 
ATTTTTCAAA AATTCATGGA AAGTGACAAG TTTACTAGAT TTTGTCAGTG GAAAAACGTG 
GAATTAAATA TCCATGTGCA GTCGTGTAAT AAACATGATG ATATCTCTTA CTGGCCCGCA 
GTTGCATGTT TTTACGGTTT TGAGAGTGAT GAAAGCGGCC TCGAAGGCGG GTTGACCATG 
AATGATTTCA GCGTGCATCG GATCATTGGA CGAGGAGGAT TCGGTGAAGT ATACGGTTGC 
AGGAAAGCAG ACACTGGAAA GATGTATGCA ATGAAATGCT TGGATAAGAA GAGAATCAAG 
ATGAAACAGG GAGAAACCTT AGCCTTAAAT GAAAGGATCA TGTTGTCCCT GGTGAGCACA 
GGAGATTGCC CTTTCATCGT CTGTATGACC TATGCCTTCC ACACTCCAGA TAAACTGTGC 
TTCATCTTGG ATCTGATGAA CGGGGGTGAC CTGCACTATC ACCTTTCGCA GCACGGGGTG 
TTTTCTGAGA AGGAGATGCG GTTTTACGCC ACAGAAATCA TCCTGGGGCT GGAACACATG 
CACAATCGGT TTGTCGTTTA CAGAGACTTG AAGCCCGCCA ATATCCTCCT GGATGAGCAC 
GGACATGTGA GGATATCAGA CCTTGGTCTT GCCTGCGATT TTTCCAAAAA GAAGCCGCAC 
GCGAGCGTGG GCACCCACGG GTACATGGCG CCCGAAGTTC TGCAGAAGGG GACCGCCTAC 
GACAGCAGTG CCGACTGGTT CTCCCTGGGC TGTATGCTTT TCAAACTTCT GAGAGGTCAC 
AGCCCTTTCA GACAACATAA AACCAAAGAT AAGCATGAGA TAGACCGAAT GACTCTCACC 
ATGAACGTGG AACTTCCAGA CGTCTTCTCC CCTGAGCTCA AGTCCCTTCT GGAAGGCCTG 
CTTCAGCGAG ATGTCAGTAA GCGCCTCGGC TGCCATGGAG GCAGCGCACA GGAGCTAAAA 
ACGCACGACT TCTTCAGAGG CATCGACTGG CAGCACGTCT ACCTGCAGAA GTACCCTCCA 
CCCTTGATCC CTCCCCGAGG GGAAGTCAAT GCAGCCGACG CCTTTGACAT CGGCTCATTT 
GATGAAGAGG ATACCAAAGG CATCAAGCTT CTTGATTGCG ACCAAGAACT CTACAAGAAC 
TTCCCTCTGG TGATCTCTGA GCGCTGGCAG CAGGAAGTGG CGGAAACAGT TTATGAAGCA 
GTAAATGCAG ACACGGATAA AATCGAGGCC AGGAAGAGAG CTAAAAATAA GCAGCTTGGC 
CACGAAGAAG ATTACGCCCT GGGAAGAGAC TGCATCGTGC ACGGGTACAT GCTGAAGCTG 
GGGAACCCTT TCCTGACCCA GTGGCAGCGC CGCTATTTTT ACCTCTTTCC GAACAGACTT 
GAGTGGAGAG GAGAAGGCGA GTCGCGACAA AGTTTACTGA CAATGGAACA GATTGTGTCC 
GTGGAAGAAA CTCAGATTAA AGACAAAAAG TGCATTTTGT TGAGAATAAA AGGAGGGAAG 
CAGTTCGTTT TGCAGTGTGA GAGTGACCCA GAGTTTGTGC AGTGGAAGAA AGAGCTGACG 
GAGACATTCA TGGAGGCCCA GCGGCTGCTA CGGCGAGCCC CCAAGTTCCT CAACAAATCC 
CGCTCAGCCG TCGTGGAACT CTCAAAGCCT CCCCTCTGCC ATAGGAACAG CAACGGCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832782.1
CDS	69..2231
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKIVLPEPSIRSVMQKYLEERHEITFDKIFNQRIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLENEEDRLCRSRQIYDTYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGSIFQKFMESDKFTRFCQWKNVELNIHVQSCNKHDDISYWPAVACFYGFESDESGLEGGLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMNVELPDVFSPELKSLLEGLLQRDVSKRLGCHGGSAQELKTHDFFRGIDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVAETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGRDCIVHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQSLLTMEQIVSVEETQIKDKKCILLRIKGGKQFVLQCESDPEFVQWKKELTETFMEAQRLLRRAPKFLNKSRSAVVELSKPPLCHRNSNGL

Target ORF information:

RefSeq Version	XM_024977014.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977014.1

ORF Insert Sequence:

ATGGCGGACC TGGAGGCCGT GCTGGCCGAT GTCAGCTACC TGATGGCGAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AAGATCGTCC TGCCCGAGCC CAGTATCCGG 
AGCGTGATGC AGAAGTATCT TGAGGAGAGA CACGAAATCA CCTTTGACAA GATTTTTAAT 
CAGAGAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAA GAATATGAAA AGCTTGAGAA TGAGGAAGAT 
CGCCTTTGTA GAAGTCGACA GATTTATGAC ACTTACATCA TGAAGGAGCT GCTGTCGTGT 
TCACATCCAT TCTCAAAGCA AGCCGTAGAA CACGTACAAA GTCATCTGTC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GCCATACATA GAAGAAATTT GTGAAAGTCT CCGAGGCAGC 
ATTTTTCAAA AATTCATGGA AAGTGACAAG TTTACTAGAT TTTGTCAGTG GAAAAACGTG 
GAATTAAATA TCCATGTGCA GTCGTGTAAT AAACATGATG ATATCTCTTA CTGGCCCGCA 
GTTGCATGTT TTTACGGTTT TGAGAGTGAT GAAAGCGGCC TCGAAGGCGG GTTGACCATG 
AATGATTTCA GCGTGCATCG GATCATTGGA CGAGGAGGAT TCGGTGAAGT ATACGGTTGC 
AGGAAAGCAG ACACTGGAAA GATGTATGCA ATGAAATGCT TGGATAAGAA GAGAATCAAG 
ATGAAACAGG GAGAAACCTT AGCCTTAAAT GAAAGGATCA TGTTGTCCCT GGTGAGCACA 
GGAGATTGCC CTTTCATCGT CTGTATGACC TATGCCTTCC ACACTCCAGA TAAACTGTGC 
TTCATCTTGG ATCTGATGAA CGGGGGTGAC CTGCACTATC ACCTTTCGCA GCACGGGGTG 
TTTTCTGAGA AGGAGATGCG GTTTTACGCC ACAGAAATCA TCCTGGGGCT GGAACACATG 
CACAATCGGT TTGTCGTTTA CAGAGACTTG AAGCCCGCCA ATATCCTCCT GGATGAGCAC 
GGACATGTGA GGATATCAGA CCTTGGTCTT GCCTGCGATT TTTCCAAAAA GAAGCCGCAC 
GCGAGCGTGG GCACCCACGG GTACATGGCG CCCGAAGTTC TGCAGAAGGG GACCGCCTAC 
GACAGCAGTG CCGACTGGTT CTCCCTGGGC TGTATGCTTT TCAAACTTCT GAGAGGTCAC 
AGCCCTTTCA GACAACATAA AACCAAAGAT AAGCATGAGA TAGACCGAAT GACTCTCACC 
ATGAACGTGG AACTTCCAGA CGTCTTCTCC CCTGAGCTCA AGTCCCTTCT GGAAGGCCTG 
CTTCAGCGAG ATGTCAGTAA GCGCCTCGGC TGCCATGGAG GCAGCGCACA GGAGCTAAAA 
ACGCACGACT TCTTCAGAGG CATCGACTGG CAGCACGTCT ACCTGCAGAA GTACCCTCCA 
CCCTTGATCC CTCCCCGAGG GGAAGTCAAT GCAGCCGACG CCTTTGACAT CGGCTCATTT 
GATGAAGAGG ATACCAAAGG CATCAAGCTT CTTGATTGCG ACCAAGAACT CTACAAGAAC 
TTCCCTCTGG TGATCTCTGA GCGCTGGCAG CAGGAAGTGG CGGAAACAGT TTATGAAGCA 
GTAAATGCAG ACACGGATAA AATCGAGGCC AGGAAGAGAG CTAAAAATAA GCAGCTTGGC 
CACGAAGAAG ATTACGCCCT GGGAAGAGAC TGCATCGTGC ACGGGTACAT GCTGAAGCTG 
GGGAACCCTT TCCTGACCCA GTGGCAGCGC CGCTATTTTT ACCTCTTTCC GAACAGACTT 
GAGTGGAGAG GAGAAGGCGA GTCGCGACAA AGTTTACTGA CAATGGAACA GATTGTGTCC 
GTGGAAGAAA CTCAGATTAA AGACAAAAAG TGCATTTTGT TGAGAATAAA AGGAGGGAAG 
CAGTTCGTTT TGCAGTGTGA GAGTGACCCA GAGTTTGTGC AGTGGAAGAA AGAGCTGACG 
GAGACATTCA TGGAGGCCCA GCGGCTGCTA CGGCGAGCCC CCAAGTTCCT CAACAAATCC 
CGCTCAGCCG TCGTGGAACT CTCAAAGCCT CCCCTCTGCC ATAGGAACAG CAACGGCCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( XM_024977016.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832784.1 Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa224150
Clone ID Related Accession (Same CDS sequence)	XM_024977016.1
Accession Version	XM_024977016.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGACC TGGAGGCCGT GCTGGCCGAT GTCAGCTACC TGATGGCGAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AAGATCGTCC TGCCCGAGCC CAGTATCCGG 
AGCGTGATGC AGAAGTATCT TGAGGAGAGA CACGAAATCA CCTTTGACAA GATTTTTAAT 
CAGAGAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAA GAATATGAAA AGCTTGAGAA TGAGGAAGAT 
CGCCTTTGTA GAAGTCGACA GATTTATGAC ACTTACATCA TGAAGGAGCT GCTGTCGTGT 
TCACATCCAT TCTCAAAGCA AGCCGTAGAA CACGTACAAA GTCATCTGTC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GTTGACCATG AATGATTTCA GCGTGCATCG GATCATTGGA 
CGAGGAGGAT TCGGTGAAGT ATACGGTTGC AGGAAAGCAG ACACTGGAAA GATGTATGCA 
ATGAAATGCT TGGATAAGAA GAGAATCAAG ATGAAACAGG GAGAAACCTT AGCCTTAAAT 
GAAAGGATCA TGTTGTCCCT GGTGAGCACA GGAGATTGCC CTTTCATCGT CTGTATGACC 
TATGCCTTCC ACACTCCAGA TAAACTGTGC TTCATCTTGG ATCTGATGAA CGGGGGTGAC 
CTGCACTATC ACCTTTCGCA GCACGGGGTG TTTTCTGAGA AGGAGATGCG GTTTTACGCC 
ACAGAAATCA TCCTGGGGCT GGAACACATG CACAATCGGT TTGTCGTTTA CAGAGACTTG 
AAGCCCGCCA ATATCCTCCT GGATGAGCAC GGACATGTGA GGATATCAGA CCTTGGTCTT 
GCCTGCGATT TTTCCAAAAA GAAGCCGCAC GCGAGCGTGG GCACCCACGG GTACATGGCG 
CCCGAAGTTC TGCAGAAGGG GACCGCCTAC GACAGCAGTG CCGACTGGTT CTCCCTGGGC 
TGTATGCTTT TCAAACTTCT GAGAGGTCAC AGCCCTTTCA GACAACATAA AACCAAAGAT 
AAGCATGAGA TAGACCGAAT GACTCTCACC ATGAACGTGG AACTTCCAGA CGTCTTCTCC 
CCTGAGCTCA AGTCCCTTCT GGAAGGCCTG CTTCAGCGAG ATGTCAGTAA GCGCCTCGGC 
TGCCATGGAG GCAGCGCACA GGAGCTAAAA ACGCACGACT TCTTCAGAGG CATCGACTGG 
CAGCACGTCT ACCTGCAGAA GTACCCTCCA CCCTTGATCC CTCCCCGAGG GGAAGTCAAT 
GCAGCCGACG CCTTTGACAT CGGCTCATTT GATGAAGAGG ATACCAAAGG CATCAAGCTT 
CTTGATTGCG ACCAAGAACT CTACAAGAAC TTCCCTCTGG TGATCTCTGA GCGCTGGCAG 
CAGGAAGTGG CGGAAACAGT TTATGAAGCA GTAAATGCAG ACACGGATAA AATCGAGGCC 
AGGAAGAGAG CTAAAAATAA GCAGCTTGGC CACGAAGAAG ATTACGCCCT GGGAAGAGAC 
TGCATCGTGC ACGGGTACAT GCTGAAGCTG GGGAACCCTT TCCTGACCCA GTGGCAGCGC 
CGCTATTTTT ACCTCTTTCC GAACAGACTT GAGTGGAGAG GAGAAGGCGA GTCGCGACAA 
AGTTTACTGA CAATGGAACA GATTGTGTCC GTGGAAGAAA CTCAGATTAA AGACAAAAAG 
TGCATTTTGT TGAGAATAAA AGGAGGGAAG CAGTTCGTTT TGCAGTGTGA GAGTGACCCA 
GAGTTTGTGC AGTGGAAGAA AGAGCTGACG GAGACATTCA TGGAGGCCCA GCGGCTGCTA 
CGGCGAGCCC CCAAGTTCCT CAACAAATCC CGCTCAGCCG TCGTGGAACT CTCAAAGCCT 
CCCCTCTGCC ATAGGAACAG CAACGGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832784.1
CDS	69..2021
Translation	MADLEAVLADVSYLMAMEKSKATPAARASKKIVLPEPSIRSVMQKYLEERHEITFDKIFNQRIGFLLFKDFCLNEINEAVPQVKFYEEIKEYEKLENEEDRLCRSRQIYDTYIMKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMNVELPDVFSPELKSLLEGLLQRDVSKRLGCHGGSAQELKTHDFFRGIDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVAETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGRDCIVHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQSLLTMEQIVSVEETQIKDKKCILLRIKGGKQFVLQCESDPEFVQWKKELTETFMEAQRLLRRAPKFLNKSRSAVVELSKPPLCHRNSNGL

Target ORF information:

RefSeq Version	XM_024977016.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977016.1

ORF Insert Sequence:

ATGGCGGACC TGGAGGCCGT GCTGGCCGAT GTCAGCTACC TGATGGCGAT GGAGAAGAGC 
AAGGCGACCC CGGCCGCCCG CGCCAGCAAG AAGATCGTCC TGCCCGAGCC CAGTATCCGG 
AGCGTGATGC AGAAGTATCT TGAGGAGAGA CACGAAATCA CCTTTGACAA GATTTTTAAT 
CAGAGAATTG GTTTCTTGCT ATTTAAAGAT TTTTGTTTGA ATGAAATTAA TGAAGCTGTA 
CCTCAGGTGA AGTTTTATGA AGAGATAAAA GAATATGAAA AGCTTGAGAA TGAGGAAGAT 
CGCCTTTGTA GAAGTCGACA GATTTATGAC ACTTACATCA TGAAGGAGCT GCTGTCGTGT 
TCACATCCAT TCTCAAAGCA AGCCGTAGAA CACGTACAAA GTCATCTGTC CAAGAAACAA 
GTGACATCAA CTCTTTTTCA GTTGACCATG AATGATTTCA GCGTGCATCG GATCATTGGA 
CGAGGAGGAT TCGGTGAAGT ATACGGTTGC AGGAAAGCAG ACACTGGAAA GATGTATGCA 
ATGAAATGCT TGGATAAGAA GAGAATCAAG ATGAAACAGG GAGAAACCTT AGCCTTAAAT 
GAAAGGATCA TGTTGTCCCT GGTGAGCACA GGAGATTGCC CTTTCATCGT CTGTATGACC 
TATGCCTTCC ACACTCCAGA TAAACTGTGC TTCATCTTGG ATCTGATGAA CGGGGGTGAC 
CTGCACTATC ACCTTTCGCA GCACGGGGTG TTTTCTGAGA AGGAGATGCG GTTTTACGCC 
ACAGAAATCA TCCTGGGGCT GGAACACATG CACAATCGGT TTGTCGTTTA CAGAGACTTG 
AAGCCCGCCA ATATCCTCCT GGATGAGCAC GGACATGTGA GGATATCAGA CCTTGGTCTT 
GCCTGCGATT TTTCCAAAAA GAAGCCGCAC GCGAGCGTGG GCACCCACGG GTACATGGCG 
CCCGAAGTTC TGCAGAAGGG GACCGCCTAC GACAGCAGTG CCGACTGGTT CTCCCTGGGC 
TGTATGCTTT TCAAACTTCT GAGAGGTCAC AGCCCTTTCA GACAACATAA AACCAAAGAT 
AAGCATGAGA TAGACCGAAT GACTCTCACC ATGAACGTGG AACTTCCAGA CGTCTTCTCC 
CCTGAGCTCA AGTCCCTTCT GGAAGGCCTG CTTCAGCGAG ATGTCAGTAA GCGCCTCGGC 
TGCCATGGAG GCAGCGCACA GGAGCTAAAA ACGCACGACT TCTTCAGAGG CATCGACTGG 
CAGCACGTCT ACCTGCAGAA GTACCCTCCA CCCTTGATCC CTCCCCGAGG GGAAGTCAAT 
GCAGCCGACG CCTTTGACAT CGGCTCATTT GATGAAGAGG ATACCAAAGG CATCAAGCTT 
CTTGATTGCG ACCAAGAACT CTACAAGAAC TTCCCTCTGG TGATCTCTGA GCGCTGGCAG 
CAGGAAGTGG CGGAAACAGT TTATGAAGCA GTAAATGCAG ACACGGATAA AATCGAGGCC 
AGGAAGAGAG CTAAAAATAA GCAGCTTGGC CACGAAGAAG ATTACGCCCT GGGAAGAGAC 
TGCATCGTGC ACGGGTACAT GCTGAAGCTG GGGAACCCTT TCCTGACCCA GTGGCAGCGC 
CGCTATTTTT ACCTCTTTCC GAACAGACTT GAGTGGAGAG GAGAAGGCGA GTCGCGACAA 
AGTTTACTGA CAATGGAACA GATTGTGTCC GTGGAAGAAA CTCAGATTAA AGACAAAAAG 
TGCATTTTGT TGAGAATAAA AGGAGGGAAG CAGTTCGTTT TGCAGTGTGA GAGTGACCCA 
GAGTTTGTGC AGTGGAAGAA AGAGCTGACG GAGACATTCA TGGAGGCCCA GCGGCTGCTA 
CGGCGAGCCC CCAAGTTCCT CAACAAATCC CGCTCAGCCG TCGTGGAACT CTCAAAGCCT 
CCCCTCTGCC ATAGGAACAG CAACGGCCTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( XM_024977018.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832786.1 Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa224151
Clone ID Related Accession (Same CDS sequence)	XM_024977018.1 , XM_024977017.1
Accession Version	XM_024977018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGC TGCTGTCGTG TTCACATCCA TTCTCAAAGC AAGCCGTAGA ACACGTACAA 
AGTCATCTGT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGTC TCCGAGGCAG CATTTTTCAA AAATTCATGG AAAGTGACAA GTTTACTAGA 
TTTTGTCAGT GGAAAAACGT GGAATTAAAT ATCCATGTGC AGTCGTGTAA TAAACATGAT 
GATATCTCTT ACTGGCCCGC AGTTGCATGT TTTTACGGTT TTGAGAGTGA TGAAAGCGGC 
CTCGAAGGCG GGTTGACCAT GAATGATTTC AGCGTGCATC GGATCATTGG ACGAGGAGGA 
TTCGGTGAAG TATACGGTTG CAGGAAAGCA GACACTGGAA AGATGTATGC AATGAAATGC 
TTGGATAAGA AGAGAATCAA GATGAAACAG GGAGAAACCT TAGCCTTAAA TGAAAGGATC 
ATGTTGTCCC TGGTGAGCAC AGGAGATTGC CCTTTCATCG TCTGTATGAC CTATGCCTTC 
CACACTCCAG ATAAACTGTG CTTCATCTTG GATCTGATGA ACGGGGGTGA CCTGCACTAT 
CACCTTTCGC AGCACGGGGT GTTTTCTGAG AAGGAGATGC GGTTTTACGC CACAGAAATC 
ATCCTGGGGC TGGAACACAT GCACAATCGG TTTGTCGTTT ACAGAGACTT GAAGCCCGCC 
AATATCCTCC TGGATGAGCA CGGACATGTG AGGATATCAG ACCTTGGTCT TGCCTGCGAT 
TTTTCCAAAA AGAAGCCGCA CGCGAGCGTG GGCACCCACG GGTACATGGC GCCCGAAGTT 
CTGCAGAAGG GGACCGCCTA CGACAGCAGT GCCGACTGGT TCTCCCTGGG CTGTATGCTT 
TTCAAACTTC TGAGAGGTCA CAGCCCTTTC AGACAACATA AAACCAAAGA TAAGCATGAG 
ATAGACCGAA TGACTCTCAC CATGAACGTG GAACTTCCAG ACGTCTTCTC CCCTGAGCTC 
AAGTCCCTTC TGGAAGGCCT GCTTCAGCGA GATGTCAGTA AGCGCCTCGG CTGCCATGGA 
GGCAGCGCAC AGGAGCTAAA AACGCACGAC TTCTTCAGAG GCATCGACTG GCAGCACGTC 
TACCTGCAGA AGTACCCTCC ACCCTTGATC CCTCCCCGAG GGGAAGTCAA TGCAGCCGAC 
GCCTTTGACA TCGGCTCATT TGATGAAGAG GATACCAAAG GCATCAAGCT TCTTGATTGC 
GACCAAGAAC TCTACAAGAA CTTCCCTCTG GTGATCTCTG AGCGCTGGCA GCAGGAAGTG 
GCGGAAACAG TTTATGAAGC AGTAAATGCA GACACGGATA AAATCGAGGC CAGGAAGAGA 
GCTAAAAATA AGCAGCTTGG CCACGAAGAA GATTACGCCC TGGGAAGAGA CTGCATCGTG 
CACGGGTACA TGCTGAAGCT GGGGAACCCT TTCCTGACCC AGTGGCAGCG CCGCTATTTT 
TACCTCTTTC CGAACAGACT TGAGTGGAGA GGAGAAGGCG AGTCGCGACA AAGTTTACTG 
ACAATGGAAC AGATTGTGTC CGTGGAAGAA ACTCAGATTA AAGACAAAAA GTGCATTTTG 
TTGAGAATAA AAGGAGGGAA GCAGTTCGTT TTGCAGTGTG AGAGTGACCC AGAGTTTGTG 
CAGTGGAAGA AAGAGCTGAC GGAGACATTC ATGGAGGCCC AGCGGCTGCT ACGGCGAGCC 
CCCAAGTTCC TCAACAAATC CCGCTCAGCC GTCGTGGAAC TCTCAAAGCC TCCCCTCTGC 
CATAGGAACA GCAACGGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832786.1
CDS	153..1976
Translation	MKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGSIFQKFMESDKFTRFCQWKNVELNIHVQSCNKHDDISYWPAVACFYGFESDESGLEGGLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMNVELPDVFSPELKSLLEGLLQRDVSKRLGCHGGSAQELKTHDFFRGIDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVAETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGRDCIVHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQSLLTMEQIVSVEETQIKDKKCILLRIKGGKQFVLQCESDPEFVQWKKELTETFMEAQRLLRRAPKFLNKSRSAVVELSKPPLCHRNSNGL

Target ORF information:

RefSeq Version	XM_024977018.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977018.1

ORF Insert Sequence:

ATGAAGGAGC TGCTGTCGTG TTCACATCCA TTCTCAAAGC AAGCCGTAGA ACACGTACAA 
AGTCATCTGT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGTC TCCGAGGCAG CATTTTTCAA AAATTCATGG AAAGTGACAA GTTTACTAGA 
TTTTGTCAGT GGAAAAACGT GGAATTAAAT ATCCATGTGC AGTCGTGTAA TAAACATGAT 
GATATCTCTT ACTGGCCCGC AGTTGCATGT TTTTACGGTT TTGAGAGTGA TGAAAGCGGC 
CTCGAAGGCG GGTTGACCAT GAATGATTTC AGCGTGCATC GGATCATTGG ACGAGGAGGA 
TTCGGTGAAG TATACGGTTG CAGGAAAGCA GACACTGGAA AGATGTATGC AATGAAATGC 
TTGGATAAGA AGAGAATCAA GATGAAACAG GGAGAAACCT TAGCCTTAAA TGAAAGGATC 
ATGTTGTCCC TGGTGAGCAC AGGAGATTGC CCTTTCATCG TCTGTATGAC CTATGCCTTC 
CACACTCCAG ATAAACTGTG CTTCATCTTG GATCTGATGA ACGGGGGTGA CCTGCACTAT 
CACCTTTCGC AGCACGGGGT GTTTTCTGAG AAGGAGATGC GGTTTTACGC CACAGAAATC 
ATCCTGGGGC TGGAACACAT GCACAATCGG TTTGTCGTTT ACAGAGACTT GAAGCCCGCC 
AATATCCTCC TGGATGAGCA CGGACATGTG AGGATATCAG ACCTTGGTCT TGCCTGCGAT 
TTTTCCAAAA AGAAGCCGCA CGCGAGCGTG GGCACCCACG GGTACATGGC GCCCGAAGTT 
CTGCAGAAGG GGACCGCCTA CGACAGCAGT GCCGACTGGT TCTCCCTGGG CTGTATGCTT 
TTCAAACTTC TGAGAGGTCA CAGCCCTTTC AGACAACATA AAACCAAAGA TAAGCATGAG 
ATAGACCGAA TGACTCTCAC CATGAACGTG GAACTTCCAG ACGTCTTCTC CCCTGAGCTC 
AAGTCCCTTC TGGAAGGCCT GCTTCAGCGA GATGTCAGTA AGCGCCTCGG CTGCCATGGA 
GGCAGCGCAC AGGAGCTAAA AACGCACGAC TTCTTCAGAG GCATCGACTG GCAGCACGTC 
TACCTGCAGA AGTACCCTCC ACCCTTGATC CCTCCCCGAG GGGAAGTCAA TGCAGCCGAC 
GCCTTTGACA TCGGCTCATT TGATGAAGAG GATACCAAAG GCATCAAGCT TCTTGATTGC 
GACCAAGAAC TCTACAAGAA CTTCCCTCTG GTGATCTCTG AGCGCTGGCA GCAGGAAGTG 
GCGGAAACAG TTTATGAAGC AGTAAATGCA GACACGGATA AAATCGAGGC CAGGAAGAGA 
GCTAAAAATA AGCAGCTTGG CCACGAAGAA GATTACGCCC TGGGAAGAGA CTGCATCGTG 
CACGGGTACA TGCTGAAGCT GGGGAACCCT TTCCTGACCC AGTGGCAGCG CCGCTATTTT 
TACCTCTTTC CGAACAGACT TGAGTGGAGA GGAGAAGGCG AGTCGCGACA AAGTTTACTG 
ACAATGGAAC AGATTGTGTC CGTGGAAGAA ACTCAGATTA AAGACAAAAA GTGCATTTTG 
TTGAGAATAA AAGGAGGGAA GCAGTTCGTT TTGCAGTGTG AGAGTGACCC AGAGTTTGTG 
CAGTGGAAGA AAGAGCTGAC GGAGACATTC ATGGAGGCCC AGCGGCTGCT ACGGCGAGCC 
CCCAAGTTCC TCAACAAATC CCGCTCAGCC GTCGTGGAAC TCTCAAAGCC TCCCCTCTGC 
CATAGGAACA GCAACGGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GRK3 ( XM_024977017.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024832785.1 Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa224151
Clone ID Related Accession (Same CDS sequence)	XM_024977018.1 , XM_024977017.1
Accession Version	XM_024977017.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	beta-adrenergic receptor kinase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGAGC TGCTGTCGTG TTCACATCCA TTCTCAAAGC AAGCCGTAGA ACACGTACAA 
AGTCATCTGT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGTC TCCGAGGCAG CATTTTTCAA AAATTCATGG AAAGTGACAA GTTTACTAGA 
TTTTGTCAGT GGAAAAACGT GGAATTAAAT ATCCATGTGC AGTCGTGTAA TAAACATGAT 
GATATCTCTT ACTGGCCCGC AGTTGCATGT TTTTACGGTT TTGAGAGTGA TGAAAGCGGC 
CTCGAAGGCG GGTTGACCAT GAATGATTTC AGCGTGCATC GGATCATTGG ACGAGGAGGA 
TTCGGTGAAG TATACGGTTG CAGGAAAGCA GACACTGGAA AGATGTATGC AATGAAATGC 
TTGGATAAGA AGAGAATCAA GATGAAACAG GGAGAAACCT TAGCCTTAAA TGAAAGGATC 
ATGTTGTCCC TGGTGAGCAC AGGAGATTGC CCTTTCATCG TCTGTATGAC CTATGCCTTC 
CACACTCCAG ATAAACTGTG CTTCATCTTG GATCTGATGA ACGGGGGTGA CCTGCACTAT 
CACCTTTCGC AGCACGGGGT GTTTTCTGAG AAGGAGATGC GGTTTTACGC CACAGAAATC 
ATCCTGGGGC TGGAACACAT GCACAATCGG TTTGTCGTTT ACAGAGACTT GAAGCCCGCC 
AATATCCTCC TGGATGAGCA CGGACATGTG AGGATATCAG ACCTTGGTCT TGCCTGCGAT 
TTTTCCAAAA AGAAGCCGCA CGCGAGCGTG GGCACCCACG GGTACATGGC GCCCGAAGTT 
CTGCAGAAGG GGACCGCCTA CGACAGCAGT GCCGACTGGT TCTCCCTGGG CTGTATGCTT 
TTCAAACTTC TGAGAGGTCA CAGCCCTTTC AGACAACATA AAACCAAAGA TAAGCATGAG 
ATAGACCGAA TGACTCTCAC CATGAACGTG GAACTTCCAG ACGTCTTCTC CCCTGAGCTC 
AAGTCCCTTC TGGAAGGCCT GCTTCAGCGA GATGTCAGTA AGCGCCTCGG CTGCCATGGA 
GGCAGCGCAC AGGAGCTAAA AACGCACGAC TTCTTCAGAG GCATCGACTG GCAGCACGTC 
TACCTGCAGA AGTACCCTCC ACCCTTGATC CCTCCCCGAG GGGAAGTCAA TGCAGCCGAC 
GCCTTTGACA TCGGCTCATT TGATGAAGAG GATACCAAAG GCATCAAGCT TCTTGATTGC 
GACCAAGAAC TCTACAAGAA CTTCCCTCTG GTGATCTCTG AGCGCTGGCA GCAGGAAGTG 
GCGGAAACAG TTTATGAAGC AGTAAATGCA GACACGGATA AAATCGAGGC CAGGAAGAGA 
GCTAAAAATA AGCAGCTTGG CCACGAAGAA GATTACGCCC TGGGAAGAGA CTGCATCGTG 
CACGGGTACA TGCTGAAGCT GGGGAACCCT TTCCTGACCC AGTGGCAGCG CCGCTATTTT 
TACCTCTTTC CGAACAGACT TGAGTGGAGA GGAGAAGGCG AGTCGCGACA AAGTTTACTG 
ACAATGGAAC AGATTGTGTC CGTGGAAGAA ACTCAGATTA AAGACAAAAA GTGCATTTTG 
TTGAGAATAA AAGGAGGGAA GCAGTTCGTT TTGCAGTGTG AGAGTGACCC AGAGTTTGTG 
CAGTGGAAGA AAGAGCTGAC GGAGACATTC ATGGAGGCCC AGCGGCTGCT ACGGCGAGCC 
CCCAAGTTCC TCAACAAATC CCGCTCAGCC GTCGTGGAAC TCTCAAAGCC TCCCCTCTGC 
CATAGGAACA GCAACGGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024832785.1
CDS	229..2052
Translation	MKELLSCSHPFSKQAVEHVQSHLSKKQVTSTLFQPYIEEICESLRGSIFQKFMESDKFTRFCQWKNVELNIHVQSCNKHDDISYWPAVACFYGFESDESGLEGGLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMTYAFHTPDKLCFILDLMNGGDLHYHLSQHGVFSEKEMRFYATEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGTAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMNVELPDVFSPELKSLLEGLLQRDVSKRLGCHGGSAQELKTHDFFRGIDWQHVYLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDCDQELYKNFPLVISERWQQEVAETVYEAVNADTDKIEARKRAKNKQLGHEEDYALGRDCIVHGYMLKLGNPFLTQWQRRYFYLFPNRLEWRGEGESRQSLLTMEQIVSVEETQIKDKKCILLRIKGGKQFVLQCESDPEFVQWKKELTETFMEAQRLLRRAPKFLNKSRSAVVELSKPPLCHRNSNGL

Target ORF information:

RefSeq Version	XM_024977017.1
Organism	Bos taurus(cattle)
Definition	Bos taurus G protein-coupled receptor kinase 3 (GRK3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024977017.1

ORF Insert Sequence:

ATGAAGGAGC TGCTGTCGTG TTCACATCCA TTCTCAAAGC AAGCCGTAGA ACACGTACAA 
AGTCATCTGT CCAAGAAACA AGTGACATCA ACTCTTTTTC AGCCATACAT AGAAGAAATT 
TGTGAAAGTC TCCGAGGCAG CATTTTTCAA AAATTCATGG AAAGTGACAA GTTTACTAGA 
TTTTGTCAGT GGAAAAACGT GGAATTAAAT ATCCATGTGC AGTCGTGTAA TAAACATGAT 
GATATCTCTT ACTGGCCCGC AGTTGCATGT TTTTACGGTT TTGAGAGTGA TGAAAGCGGC 
CTCGAAGGCG GGTTGACCAT GAATGATTTC AGCGTGCATC GGATCATTGG ACGAGGAGGA 
TTCGGTGAAG TATACGGTTG CAGGAAAGCA GACACTGGAA AGATGTATGC AATGAAATGC 
TTGGATAAGA AGAGAATCAA GATGAAACAG GGAGAAACCT TAGCCTTAAA TGAAAGGATC 
ATGTTGTCCC TGGTGAGCAC AGGAGATTGC CCTTTCATCG TCTGTATGAC CTATGCCTTC 
CACACTCCAG ATAAACTGTG CTTCATCTTG GATCTGATGA ACGGGGGTGA CCTGCACTAT 
CACCTTTCGC AGCACGGGGT GTTTTCTGAG AAGGAGATGC GGTTTTACGC CACAGAAATC 
ATCCTGGGGC TGGAACACAT GCACAATCGG TTTGTCGTTT ACAGAGACTT GAAGCCCGCC 
AATATCCTCC TGGATGAGCA CGGACATGTG AGGATATCAG ACCTTGGTCT TGCCTGCGAT 
TTTTCCAAAA AGAAGCCGCA CGCGAGCGTG GGCACCCACG GGTACATGGC GCCCGAAGTT 
CTGCAGAAGG GGACCGCCTA CGACAGCAGT GCCGACTGGT TCTCCCTGGG CTGTATGCTT 
TTCAAACTTC TGAGAGGTCA CAGCCCTTTC AGACAACATA AAACCAAAGA TAAGCATGAG 
ATAGACCGAA TGACTCTCAC CATGAACGTG GAACTTCCAG ACGTCTTCTC CCCTGAGCTC 
AAGTCCCTTC TGGAAGGCCT GCTTCAGCGA GATGTCAGTA AGCGCCTCGG CTGCCATGGA 
GGCAGCGCAC AGGAGCTAAA AACGCACGAC TTCTTCAGAG GCATCGACTG GCAGCACGTC 
TACCTGCAGA AGTACCCTCC ACCCTTGATC CCTCCCCGAG GGGAAGTCAA TGCAGCCGAC 
GCCTTTGACA TCGGCTCATT TGATGAAGAG GATACCAAAG GCATCAAGCT TCTTGATTGC 
GACCAAGAAC TCTACAAGAA CTTCCCTCTG GTGATCTCTG AGCGCTGGCA GCAGGAAGTG 
GCGGAAACAG TTTATGAAGC AGTAAATGCA GACACGGATA AAATCGAGGC CAGGAAGAGA 
GCTAAAAATA AGCAGCTTGG CCACGAAGAA GATTACGCCC TGGGAAGAGA CTGCATCGTG 
CACGGGTACA TGCTGAAGCT GGGGAACCCT TTCCTGACCC AGTGGCAGCG CCGCTATTTT 
TACCTCTTTC CGAACAGACT TGAGTGGAGA GGAGAAGGCG AGTCGCGACA AAGTTTACTG 
ACAATGGAAC AGATTGTGTC CGTGGAAGAA ACTCAGATTA AAGACAAAAA GTGCATTTTG 
TTGAGAATAA AAGGAGGGAA GCAGTTCGTT TTGCAGTGTG AGAGTGACCC AGAGTTTGTG 
CAGTGGAAGA AAGAGCTGAC GGAGACATTC ATGGAGGCCC AGCGGCTGCT ACGGCGAGCC 
CCCAAGTTCC TCAACAAATC CCGCTCAGCC GTCGTGGAAC TCTCAAAGCC TCCCCTCTGC 
CATAGGAACA GCAACGGCCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The c-terminus of GRK3 indicates rapid dissociation of G protein heterotrimers.
Cellular signalling21(6)1015-21(2009 Jun)
Hollins B,Kuravi S,Digby GJ,Lambert NA

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cloning, expression, and chromosomal localization of beta-adrenergic receptor kinase 2. A new member of the receptor kinase family.
The Journal of biological chemistry266(23)14939-46(1991 Aug)
Benovic JL,Onorato JJ,Arriza JL,Stone WC,Lohse M,Jenkins NA,Gilbert DJ,Copeland NG,Caron MG,Lefkowitz RJ

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GRK3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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