PARP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PARP1
Entrez Gene ID	142
Full Name	poly(ADP-ribose) polymerase 1
Synonyms	ADPRT,ADPRT 1,ADPRT1,ARTD1,PARP,PARP-1,PPOL,pADPRT-1
General protein information	Preferred Names poly(ADP-ribose) polymerase 1 Names poly [ADP-ribose] polymerase 1 ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) ADP-ribosyltransferase NAD(+) ADP-ribosyltransferase diphtheria toxin-like 1 NAD(+) ADP-ribosyltransferase 1 poly (ADP-ribose) polymerase family, member 1 poly(ADP-ribose) synthetase poly(ADP-ribosyl)transferase poly[ADP-ribose] synthase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.12
Summary	This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].
Disorder MIM:	173870

mRNA and Protein(s)

mRNA	Protein	Name
NM_001618.3	NP_001609.2	poly [ADP-ribose] polymerase 1
NM_001618.4	NP_001609.2	poly [ADP-ribose] polymerase 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Parp	NP_001104452.1
Arabidopsis thaliana (thale cress)	PARP1	NP_850165.1
Oryza sativa (rice)	Os07g0413700	NP_001059453.1
Homo sapiens (human)	PARP1	NP_001609.2
Pan troglodytes (chimpanzee)	PARP1	XP_003949754.1
Canis lupus familiaris (dog)	PARP1	XP_547506.3
Bos taurus (cattle)	PARP1	NP_777176.1
	PARP1	XP_001090984.1
Gallus gallus (chicken)	PARP1	NP_990594.1
Xenopus tropicalis (tropical clawed frog)	parp1	XP_002941174.2
Danio rerio (zebrafish)	parp1	NP_001038407.1
Caenorhabditis elegans (roundworm)	pme-1	NP_491072.1
Mus musculus (house mouse)	Parp1	NP_031441.2
Rattus norvegicus (Norway rat)	Parp1	NP_037195.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003230	XP_312938.4

Gene Ontology

The following PARP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PARP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25551	NM_001618.4 Latest version!	Homo sapiens poly(ADP-ribose) polymerase 1 (PARP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
View Old Accession Versions >>
OHu25551	NM_001618.3	Homo sapiens poly(ADP-ribose) polymerase 1 (PARP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PARP1 ( NM_001618.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001609.2 Homo sapiens poly(ADP-ribose) polymerase 1 (PARP1), mRNA.

Price & Availability↑

CloneID	OHu25551
Clone ID Related Accession (Same CDS sequence)	NM_001618.3 , NM_001618.4
Accession Version	NM_001618.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-12
Organism	Homo sapiens(human)
Product	poly [ADP-ribose] polymerase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097441.1, BC037545.1, J03473.1 and AK225654.1. On Aug 28, 2007 this sequence version replaced NM_001618.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225654.1, SRR1803615.193403.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCGGAGT CTTCGGATAA GCTCTATCGA GTCGAGTACG CCAAGAGCGG GCGCGCCTCT 
TGCAAGAAAT GCAGCGAGAG CATCCCCAAG GACTCGCTCC GGATGGCCAT CATGGTGCAG 
TCGCCCATGT TTGATGGAAA AGTCCCACAC TGGTACCACT TCTCCTGCTT CTGGAAGGTG 
GGCCACTCCA TCCGGCACCC TGACGTTGAG GTGGATGGGT TCTCTGAGCT TCGGTGGGAT 
GACCAGCAGA AAGTCAAGAA GACAGCGGAA GCTGGAGGAG TGACAGGCAA AGGCCAGGAT 
GGAATTGGTA GCAAGGCAGA GAAGACTCTG GGTGACTTTG CAGCAGAGTA TGCCAAGTCC 
AACAGAAGTA CGTGCAAGGG GTGTATGGAG AAGATAGAAA AGGGCCAGGT GCGCCTGTCC 
AAGAAGATGG TGGACCCGGA GAAGCCACAG CTAGGCATGA TTGACCGCTG GTACCATCCA 
GGCTGCTTTG TCAAGAACAG GGAGGAGCTG GGTTTCCGGC CCGAGTACAG TGCGAGTCAG 
CTCAAGGGCT TCAGCCTCCT TGCTACAGAG GATAAAGAAG CCCTGAAGAA GCAGCTCCCA 
GGAGTCAAGA GTGAAGGAAA GAGAAAAGGC GATGAGGTGG ATGGAGTGGA TGAAGTGGCG 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GATAGTAAGC TTGAAAAAGC CCTAAAGGCT 
CAGAACGACC TGATCTGGAA CATCAAGGAC GAGCTAAAGA AAGTGTGTTC AACTAATGAC 
CTGAAGGAGC TACTCATCTT CAACAAGCAG CAAGTGCCTT CTGGGGAGTC GGCGATCTTG 
GACCGAGTAG CTGATGGCAT GGTGTTCGGT GCCCTCCTTC CCTGCGAGGA ATGCTCGGGT 
CAGCTGGTCT TCAAGAGCGA TGCCTATTAC TGCACTGGGG ACGTCACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC ACCCAACCGG AAGGAGTGGG TAACCCCAAA GGAATTCCGA 
GAAATCTCTT ACCTCAAGAA ATTGAAGGTT AAAAAACAGG ACCGTATATT CCCCCCAGAA 
ACCAGCGCCT CCGTGGCGGC CACGCCTCCG CCCTCCACAG CCTCGGCTCC TGCTGCTGTG 
AACTCCTCTG CTTCAGCAGA TAAGCCATTA TCCAACATGA AGATCCTGAC TCTCGGGAAG 
CTGTCCCGGA ACAAGGATGA AGTGAAGGCC ATGATTGAGA AACTCGGGGG GAAGTTGACG 
GGGACGGCCA ACAAGGCTTC CCTGTGCATC AGCACCAAAA AGGAGGTGGA AAAGATGAAT 
AAGAAGATGG AGGAAGTAAA GGAAGCCAAC ATCCGAGTTG TGTCTGAGGA CTTCCTCCAG 
GACGTCTCCG CCTCCACCAA GAGCCTTCAG GAGTTGTTCT TAGCGCACAT CTTGTCCCCT 
TGGGGGGCAG AGGTGAAGGC AGAGCCTGTT GAAGTTGTGG CCCCAAGAGG GAAGTCAGGG 
GCTGCGCTCT CCAAAAAAAG CAAGGGCCAG GTCAAGGAGG AAGGTATCAA CAAATCTGAA 
AAGAGAATGA AATTAACTCT TAAAGGAGGA GCAGCTGTGG ATCCTGATTC TGGACTGGAA 
CACTCTGCGC ATGTCCTGGA GAAAGGTGGG AAGGTCTTCA GTGCCACCCT TGGCCTGGTG 
GACATCGTTA AAGGAACCAA CTCCTACTAC AAGCTGCAGC TTCTGGAGGA CGACAAGGAA 
AACAGGTATT GGATATTCAG GTCCTGGGGC CGTGTGGGTA CGGTGATCGG TAGCAACAAA 
CTGGAACAGA TGCCGTCCAA GGAGGATGCC ATTGAGCACT TCATGAAATT ATATGAAGAA 
AAAACCGGGA ACGCTTGGCA CTCCAAAAAT TTCACGAAGT ATCCCAAAAA GTTCTACCCC 
CTGGAGATTG ACTATGGCCA GGATGAAGAG GCAGTGAAGA AGCTGACAGT AAATCCTGGC 
ACCAAGTCCA AGCTCCCCAA GCCAGTTCAG GACCTCATCA AGATGATCTT TGATGTGGAA 
AGTATGAAGA AAGCCATGGT GGAGTATGAG ATCGACCTTC AGAAGATGCC CTTGGGGAAG 
CTGAGCAAAA GGCAGATCCA GGCCGCATAC TCCATCCTCA GTGAGGTCCA GCAGGCGGTG 
TCTCAGGGCA GCAGCGACTC TCAGATCCTG GATCTCTCAA ATCGCTTTTA CACCCTGATC 
CCCCACGACT TTGGGATGAA GAAGCCTCCG CTCCTGAACA ATGCAGACAG TGTGCAGGCC 
AAGGTGGAAA TGCTTGACAA CCTGCTGGAC ATCGAGGTGG CCTACAGTCT GCTCAGGGGA 
GGGTCTGATG ATAGCAGCAA GGATCCCATC GATGTCAACT ATGAGAAGCT CAAAACTGAC 
ATTAAGGTGG TTGACAGAGA TTCTGAAGAA GCCGAGATCA TCAGGAAGTA TGTTAAGAAC 
ACTCATGCAA CCACACACAA TGCGTATGAC TTGGAAGTCA TCGATATCTT TAAGATAGAG 
CGTGAAGGCG AATGCCAGCG TTACAAGCCC TTTAAGCAGC TTCATAACCG AAGATTGCTG 
TGGCACGGGT CCAGGACCAC CAACTTTGCT GGGATCCTGT CCCAGGGTCT TCGGATAGCC 
CCGCCTGAAG CGCCCGTGAC AGGCTACATG TTTGGTAAAG GGATCTATTT CGCTGACATG 
GTCTCCAAGA GTGCCAACTA CTGCCATACG TCTCAGGGAG ACCCAATAGG CTTAATCCTG 
TTGGGAGAAG TTGCCCTTGG AAACATGTAT GAACTGAAGC ACGCTTCACA TATCAGCAAG 
TTACCCAAGG GCAAGCACAG TGTCAAAGGT TTGGGCAAAA CTACCCCTGA TCCTTCAGCT 
AACATTAGTC TGGATGGTGT AGACGTTCCT CTTGGGACCG GGATTTCATC TGGTGTGAAT 
GACACCTCTC TACTATATAA CGAGTACATT GTCTATGATA TTGCTCAGGT AAATCTGAAG 
TATCTGCTGA AACTGAAATT CAATTTTAAG ACCTCCCTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001609.2
CDS	172..3216
Translation	MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

Target ORF information:

RefSeq Version	NM_001618.3
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(ADP-ribose) polymerase 1 (PARP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001618.3

ORF Insert Sequence:

ATGGCGGAGT CTTCGGATAA GCTCTATCGA GTCGAGTACG CCAAGAGCGG GCGCGCCTCT 
TGCAAGAAAT GCAGCGAGAG CATCCCCAAG GACTCGCTCC GGATGGCCAT CATGGTGCAG 
TCGCCCATGT TTGATGGAAA AGTCCCACAC TGGTACCACT TCTCCTGCTT CTGGAAGGTG 
GGCCACTCCA TCCGGCACCC TGACGTTGAG GTGGATGGGT TCTCTGAGCT TCGGTGGGAT 
GACCAGCAGA AAGTCAAGAA GACAGCGGAA GCTGGAGGAG TGACAGGCAA AGGCCAGGAT 
GGAATTGGTA GCAAGGCAGA GAAGACTCTG GGTGACTTTG CAGCAGAGTA TGCCAAGTCC 
AACAGAAGTA CGTGCAAGGG GTGTATGGAG AAGATAGAAA AGGGCCAGGT GCGCCTGTCC 
AAGAAGATGG TGGACCCGGA GAAGCCACAG CTAGGCATGA TTGACCGCTG GTACCATCCA 
GGCTGCTTTG TCAAGAACAG GGAGGAGCTG GGTTTCCGGC CCGAGTACAG TGCGAGTCAG 
CTCAAGGGCT TCAGCCTCCT TGCTACAGAG GATAAAGAAG CCCTGAAGAA GCAGCTCCCA 
GGAGTCAAGA GTGAAGGAAA GAGAAAAGGC GATGAGGTGG ATGGAGTGGA TGAAGTGGCG 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GATAGTAAGC TTGAAAAAGC CCTAAAGGCT 
CAGAACGACC TGATCTGGAA CATCAAGGAC GAGCTAAAGA AAGTGTGTTC AACTAATGAC 
CTGAAGGAGC TACTCATCTT CAACAAGCAG CAAGTGCCTT CTGGGGAGTC GGCGATCTTG 
GACCGAGTAG CTGATGGCAT GGTGTTCGGT GCCCTCCTTC CCTGCGAGGA ATGCTCGGGT 
CAGCTGGTCT TCAAGAGCGA TGCCTATTAC TGCACTGGGG ACGTCACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC ACCCAACCGG AAGGAGTGGG TAACCCCAAA GGAATTCCGA 
GAAATCTCTT ACCTCAAGAA ATTGAAGGTT AAAAAACAGG ACCGTATATT CCCCCCAGAA 
ACCAGCGCCT CCGTGGCGGC CACGCCTCCG CCCTCCACAG CCTCGGCTCC TGCTGCTGTG 
AACTCCTCTG CTTCAGCAGA TAAGCCATTA TCCAACATGA AGATCCTGAC TCTCGGGAAG 
CTGTCCCGGA ACAAGGATGA AGTGAAGGCC ATGATTGAGA AACTCGGGGG GAAGTTGACG 
GGGACGGCCA ACAAGGCTTC CCTGTGCATC AGCACCAAAA AGGAGGTGGA AAAGATGAAT 
AAGAAGATGG AGGAAGTAAA GGAAGCCAAC ATCCGAGTTG TGTCTGAGGA CTTCCTCCAG 
GACGTCTCCG CCTCCACCAA GAGCCTTCAG GAGTTGTTCT TAGCGCACAT CTTGTCCCCT 
TGGGGGGCAG AGGTGAAGGC AGAGCCTGTT GAAGTTGTGG CCCCAAGAGG GAAGTCAGGG 
GCTGCGCTCT CCAAAAAAAG CAAGGGCCAG GTCAAGGAGG AAGGTATCAA CAAATCTGAA 
AAGAGAATGA AATTAACTCT TAAAGGAGGA GCAGCTGTGG ATCCTGATTC TGGACTGGAA 
CACTCTGCGC ATGTCCTGGA GAAAGGTGGG AAGGTCTTCA GTGCCACCCT TGGCCTGGTG 
GACATCGTTA AAGGAACCAA CTCCTACTAC AAGCTGCAGC TTCTGGAGGA CGACAAGGAA 
AACAGGTATT GGATATTCAG GTCCTGGGGC CGTGTGGGTA CGGTGATCGG TAGCAACAAA 
CTGGAACAGA TGCCGTCCAA GGAGGATGCC ATTGAGCACT TCATGAAATT ATATGAAGAA 
AAAACCGGGA ACGCTTGGCA CTCCAAAAAT TTCACGAAGT ATCCCAAAAA GTTCTACCCC 
CTGGAGATTG ACTATGGCCA GGATGAAGAG GCAGTGAAGA AGCTGACAGT AAATCCTGGC 
ACCAAGTCCA AGCTCCCCAA GCCAGTTCAG GACCTCATCA AGATGATCTT TGATGTGGAA 
AGTATGAAGA AAGCCATGGT GGAGTATGAG ATCGACCTTC AGAAGATGCC CTTGGGGAAG 
CTGAGCAAAA GGCAGATCCA GGCCGCATAC TCCATCCTCA GTGAGGTCCA GCAGGCGGTG 
TCTCAGGGCA GCAGCGACTC TCAGATCCTG GATCTCTCAA ATCGCTTTTA CACCCTGATC 
CCCCACGACT TTGGGATGAA GAAGCCTCCG CTCCTGAACA ATGCAGACAG TGTGCAGGCC 
AAGGTGGAAA TGCTTGACAA CCTGCTGGAC ATCGAGGTGG CCTACAGTCT GCTCAGGGGA 
GGGTCTGATG ATAGCAGCAA GGATCCCATC GATGTCAACT ATGAGAAGCT CAAAACTGAC 
ATTAAGGTGG TTGACAGAGA TTCTGAAGAA GCCGAGATCA TCAGGAAGTA TGTTAAGAAC 
ACTCATGCAA CCACACACAA TGCGTATGAC TTGGAAGTCA TCGATATCTT TAAGATAGAG 
CGTGAAGGCG AATGCCAGCG TTACAAGCCC TTTAAGCAGC TTCATAACCG AAGATTGCTG 
TGGCACGGGT CCAGGACCAC CAACTTTGCT GGGATCCTGT CCCAGGGTCT TCGGATAGCC 
CCGCCTGAAG CGCCCGTGAC AGGCTACATG TTTGGTAAAG GGATCTATTT CGCTGACATG 
GTCTCCAAGA GTGCCAACTA CTGCCATACG TCTCAGGGAG ACCCAATAGG CTTAATCCTG 
TTGGGAGAAG TTGCCCTTGG AAACATGTAT GAACTGAAGC ACGCTTCACA TATCAGCAAG 
TTACCCAAGG GCAAGCACAG TGTCAAAGGT TTGGGCAAAA CTACCCCTGA TCCTTCAGCT 
AACATTAGTC TGGATGGTGT AGACGTTCCT CTTGGGACCG GGATTTCATC TGGTGTGAAT 
GACACCTCTC TACTATATAA CGAGTACATT GTCTATGATA TTGCTCAGGT AAATCTGAAG 
TATCTGCTGA AACTGAAATT CAATTTTAAG ACCTCCCTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP1 ( NM_001618.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001609.2 Homo sapiens poly(ADP-ribose) polymerase 1 (PARP1), mRNA.

Price & Availability↑

CloneID	OHu25551
Clone ID Related Accession (Same CDS sequence)	NM_001618.3 , NM_001618.4
Accession Version	NM_001618.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	poly [ADP-ribose] polymerase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB097441.1, BC037545.1, J03473.1 and AK225654.1. On Nov 22, 2018 this sequence version replaced NM_001618.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225654.1, SRR1803613.273515.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366794.10/ ENSP00000355759.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGAGT CTTCGGATAA GCTCTATCGA GTCGAGTACG CCAAGAGCGG GCGCGCCTCT 
TGCAAGAAAT GCAGCGAGAG CATCCCCAAG GACTCGCTCC GGATGGCCAT CATGGTGCAG 
TCGCCCATGT TTGATGGAAA AGTCCCACAC TGGTACCACT TCTCCTGCTT CTGGAAGGTG 
GGCCACTCCA TCCGGCACCC TGACGTTGAG GTGGATGGGT TCTCTGAGCT TCGGTGGGAT 
GACCAGCAGA AAGTCAAGAA GACAGCGGAA GCTGGAGGAG TGACAGGCAA AGGCCAGGAT 
GGAATTGGTA GCAAGGCAGA GAAGACTCTG GGTGACTTTG CAGCAGAGTA TGCCAAGTCC 
AACAGAAGTA CGTGCAAGGG GTGTATGGAG AAGATAGAAA AGGGCCAGGT GCGCCTGTCC 
AAGAAGATGG TGGACCCGGA GAAGCCACAG CTAGGCATGA TTGACCGCTG GTACCATCCA 
GGCTGCTTTG TCAAGAACAG GGAGGAGCTG GGTTTCCGGC CCGAGTACAG TGCGAGTCAG 
CTCAAGGGCT TCAGCCTCCT TGCTACAGAG GATAAAGAAG CCCTGAAGAA GCAGCTCCCA 
GGAGTCAAGA GTGAAGGAAA GAGAAAAGGC GATGAGGTGG ATGGAGTGGA TGAAGTGGCG 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GATAGTAAGC TTGAAAAAGC CCTAAAGGCT 
CAGAACGACC TGATCTGGAA CATCAAGGAC GAGCTAAAGA AAGTGTGTTC AACTAATGAC 
CTGAAGGAGC TACTCATCTT CAACAAGCAG CAAGTGCCTT CTGGGGAGTC GGCGATCTTG 
GACCGAGTAG CTGATGGCAT GGTGTTCGGT GCCCTCCTTC CCTGCGAGGA ATGCTCGGGT 
CAGCTGGTCT TCAAGAGCGA TGCCTATTAC TGCACTGGGG ACGTCACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC ACCCAACCGG AAGGAGTGGG TAACCCCAAA GGAATTCCGA 
GAAATCTCTT ACCTCAAGAA ATTGAAGGTT AAAAAACAGG ACCGTATATT CCCCCCAGAA 
ACCAGCGCCT CCGTGGCGGC CACGCCTCCG CCCTCCACAG CCTCGGCTCC TGCTGCTGTG 
AACTCCTCTG CTTCAGCAGA TAAGCCATTA TCCAACATGA AGATCCTGAC TCTCGGGAAG 
CTGTCCCGGA ACAAGGATGA AGTGAAGGCC ATGATTGAGA AACTCGGGGG GAAGTTGACG 
GGGACGGCCA ACAAGGCTTC CCTGTGCATC AGCACCAAAA AGGAGGTGGA AAAGATGAAT 
AAGAAGATGG AGGAAGTAAA GGAAGCCAAC ATCCGAGTTG TGTCTGAGGA CTTCCTCCAG 
GACGTCTCCG CCTCCACCAA GAGCCTTCAG GAGTTGTTCT TAGCGCACAT CTTGTCCCCT 
TGGGGGGCAG AGGTGAAGGC AGAGCCTGTT GAAGTTGTGG CCCCAAGAGG GAAGTCAGGG 
GCTGCGCTCT CCAAAAAAAG CAAGGGCCAG GTCAAGGAGG AAGGTATCAA CAAATCTGAA 
AAGAGAATGA AATTAACTCT TAAAGGAGGA GCAGCTGTGG ATCCTGATTC TGGACTGGAA 
CACTCTGCGC ATGTCCTGGA GAAAGGTGGG AAGGTCTTCA GTGCCACCCT TGGCCTGGTG 
GACATCGTTA AAGGAACCAA CTCCTACTAC AAGCTGCAGC TTCTGGAGGA CGACAAGGAA 
AACAGGTATT GGATATTCAG GTCCTGGGGC CGTGTGGGTA CGGTGATCGG TAGCAACAAA 
CTGGAACAGA TGCCGTCCAA GGAGGATGCC ATTGAGCACT TCATGAAATT ATATGAAGAA 
AAAACCGGGA ACGCTTGGCA CTCCAAAAAT TTCACGAAGT ATCCCAAAAA GTTCTACCCC 
CTGGAGATTG ACTATGGCCA GGATGAAGAG GCAGTGAAGA AGCTGACAGT AAATCCTGGC 
ACCAAGTCCA AGCTCCCCAA GCCAGTTCAG GACCTCATCA AGATGATCTT TGATGTGGAA 
AGTATGAAGA AAGCCATGGT GGAGTATGAG ATCGACCTTC AGAAGATGCC CTTGGGGAAG 
CTGAGCAAAA GGCAGATCCA GGCCGCATAC TCCATCCTCA GTGAGGTCCA GCAGGCGGTG 
TCTCAGGGCA GCAGCGACTC TCAGATCCTG GATCTCTCAA ATCGCTTTTA CACCCTGATC 
CCCCACGACT TTGGGATGAA GAAGCCTCCG CTCCTGAACA ATGCAGACAG TGTGCAGGCC 
AAGGTGGAAA TGCTTGACAA CCTGCTGGAC ATCGAGGTGG CCTACAGTCT GCTCAGGGGA 
GGGTCTGATG ATAGCAGCAA GGATCCCATC GATGTCAACT ATGAGAAGCT CAAAACTGAC 
ATTAAGGTGG TTGACAGAGA TTCTGAAGAA GCCGAGATCA TCAGGAAGTA TGTTAAGAAC 
ACTCATGCAA CCACACACAA TGCGTATGAC TTGGAAGTCA TCGATATCTT TAAGATAGAG 
CGTGAAGGCG AATGCCAGCG TTACAAGCCC TTTAAGCAGC TTCATAACCG AAGATTGCTG 
TGGCACGGGT CCAGGACCAC CAACTTTGCT GGGATCCTGT CCCAGGGTCT TCGGATAGCC 
CCGCCTGAAG CGCCCGTGAC AGGCTACATG TTTGGTAAAG GGATCTATTT CGCTGACATG 
GTCTCCAAGA GTGCCAACTA CTGCCATACG TCTCAGGGAG ACCCAATAGG CTTAATCCTG 
TTGGGAGAAG TTGCCCTTGG AAACATGTAT GAACTGAAGC ACGCTTCACA TATCAGCAAG 
TTACCCAAGG GCAAGCACAG TGTCAAAGGT TTGGGCAAAA CTACCCCTGA TCCTTCAGCT 
AACATTAGTC TGGATGGTGT AGACGTTCCT CTTGGGACCG GGATTTCATC TGGTGTGAAT 
GACACCTCTC TACTATATAA CGAGTACATT GTCTATGATA TTGCTCAGGT AAATCTGAAG 
TATCTGCTGA AACTGAAATT CAATTTTAAG ACCTCCCTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001609.2
CDS	165..3209
Translation	MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW

Target ORF information:

RefSeq Version	NM_001618.4
Organism	Homo sapiens(human)
Definition	Homo sapiens poly(ADP-ribose) polymerase 1 (PARP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001618.4

ORF Insert Sequence:

ATGGCGGAGT CTTCGGATAA GCTCTATCGA GTCGAGTACG CCAAGAGCGG GCGCGCCTCT 
TGCAAGAAAT GCAGCGAGAG CATCCCCAAG GACTCGCTCC GGATGGCCAT CATGGTGCAG 
TCGCCCATGT TTGATGGAAA AGTCCCACAC TGGTACCACT TCTCCTGCTT CTGGAAGGTG 
GGCCACTCCA TCCGGCACCC TGACGTTGAG GTGGATGGGT TCTCTGAGCT TCGGTGGGAT 
GACCAGCAGA AAGTCAAGAA GACAGCGGAA GCTGGAGGAG TGACAGGCAA AGGCCAGGAT 
GGAATTGGTA GCAAGGCAGA GAAGACTCTG GGTGACTTTG CAGCAGAGTA TGCCAAGTCC 
AACAGAAGTA CGTGCAAGGG GTGTATGGAG AAGATAGAAA AGGGCCAGGT GCGCCTGTCC 
AAGAAGATGG TGGACCCGGA GAAGCCACAG CTAGGCATGA TTGACCGCTG GTACCATCCA 
GGCTGCTTTG TCAAGAACAG GGAGGAGCTG GGTTTCCGGC CCGAGTACAG TGCGAGTCAG 
CTCAAGGGCT TCAGCCTCCT TGCTACAGAG GATAAAGAAG CCCTGAAGAA GCAGCTCCCA 
GGAGTCAAGA GTGAAGGAAA GAGAAAAGGC GATGAGGTGG ATGGAGTGGA TGAAGTGGCG 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GATAGTAAGC TTGAAAAAGC CCTAAAGGCT 
CAGAACGACC TGATCTGGAA CATCAAGGAC GAGCTAAAGA AAGTGTGTTC AACTAATGAC 
CTGAAGGAGC TACTCATCTT CAACAAGCAG CAAGTGCCTT CTGGGGAGTC GGCGATCTTG 
GACCGAGTAG CTGATGGCAT GGTGTTCGGT GCCCTCCTTC CCTGCGAGGA ATGCTCGGGT 
CAGCTGGTCT TCAAGAGCGA TGCCTATTAC TGCACTGGGG ACGTCACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC ACCCAACCGG AAGGAGTGGG TAACCCCAAA GGAATTCCGA 
GAAATCTCTT ACCTCAAGAA ATTGAAGGTT AAAAAACAGG ACCGTATATT CCCCCCAGAA 
ACCAGCGCCT CCGTGGCGGC CACGCCTCCG CCCTCCACAG CCTCGGCTCC TGCTGCTGTG 
AACTCCTCTG CTTCAGCAGA TAAGCCATTA TCCAACATGA AGATCCTGAC TCTCGGGAAG 
CTGTCCCGGA ACAAGGATGA AGTGAAGGCC ATGATTGAGA AACTCGGGGG GAAGTTGACG 
GGGACGGCCA ACAAGGCTTC CCTGTGCATC AGCACCAAAA AGGAGGTGGA AAAGATGAAT 
AAGAAGATGG AGGAAGTAAA GGAAGCCAAC ATCCGAGTTG TGTCTGAGGA CTTCCTCCAG 
GACGTCTCCG CCTCCACCAA GAGCCTTCAG GAGTTGTTCT TAGCGCACAT CTTGTCCCCT 
TGGGGGGCAG AGGTGAAGGC AGAGCCTGTT GAAGTTGTGG CCCCAAGAGG GAAGTCAGGG 
GCTGCGCTCT CCAAAAAAAG CAAGGGCCAG GTCAAGGAGG AAGGTATCAA CAAATCTGAA 
AAGAGAATGA AATTAACTCT TAAAGGAGGA GCAGCTGTGG ATCCTGATTC TGGACTGGAA 
CACTCTGCGC ATGTCCTGGA GAAAGGTGGG AAGGTCTTCA GTGCCACCCT TGGCCTGGTG 
GACATCGTTA AAGGAACCAA CTCCTACTAC AAGCTGCAGC TTCTGGAGGA CGACAAGGAA 
AACAGGTATT GGATATTCAG GTCCTGGGGC CGTGTGGGTA CGGTGATCGG TAGCAACAAA 
CTGGAACAGA TGCCGTCCAA GGAGGATGCC ATTGAGCACT TCATGAAATT ATATGAAGAA 
AAAACCGGGA ACGCTTGGCA CTCCAAAAAT TTCACGAAGT ATCCCAAAAA GTTCTACCCC 
CTGGAGATTG ACTATGGCCA GGATGAAGAG GCAGTGAAGA AGCTGACAGT AAATCCTGGC 
ACCAAGTCCA AGCTCCCCAA GCCAGTTCAG GACCTCATCA AGATGATCTT TGATGTGGAA 
AGTATGAAGA AAGCCATGGT GGAGTATGAG ATCGACCTTC AGAAGATGCC CTTGGGGAAG 
CTGAGCAAAA GGCAGATCCA GGCCGCATAC TCCATCCTCA GTGAGGTCCA GCAGGCGGTG 
TCTCAGGGCA GCAGCGACTC TCAGATCCTG GATCTCTCAA ATCGCTTTTA CACCCTGATC 
CCCCACGACT TTGGGATGAA GAAGCCTCCG CTCCTGAACA ATGCAGACAG TGTGCAGGCC 
AAGGTGGAAA TGCTTGACAA CCTGCTGGAC ATCGAGGTGG CCTACAGTCT GCTCAGGGGA 
GGGTCTGATG ATAGCAGCAA GGATCCCATC GATGTCAACT ATGAGAAGCT CAAAACTGAC 
ATTAAGGTGG TTGACAGAGA TTCTGAAGAA GCCGAGATCA TCAGGAAGTA TGTTAAGAAC 
ACTCATGCAA CCACACACAA TGCGTATGAC TTGGAAGTCA TCGATATCTT TAAGATAGAG 
CGTGAAGGCG AATGCCAGCG TTACAAGCCC TTTAAGCAGC TTCATAACCG AAGATTGCTG 
TGGCACGGGT CCAGGACCAC CAACTTTGCT GGGATCCTGT CCCAGGGTCT TCGGATAGCC 
CCGCCTGAAG CGCCCGTGAC AGGCTACATG TTTGGTAAAG GGATCTATTT CGCTGACATG 
GTCTCCAAGA GTGCCAACTA CTGCCATACG TCTCAGGGAG ACCCAATAGG CTTAATCCTG 
TTGGGAGAAG TTGCCCTTGG AAACATGTAT GAACTGAAGC ACGCTTCACA TATCAGCAAG 
TTACCCAAGG GCAAGCACAG TGTCAAAGGT TTGGGCAAAA CTACCCCTGA TCCTTCAGCT 
AACATTAGTC TGGATGGTGT AGACGTTCCT CTTGGGACCG GGATTTCATC TGGTGTGAAT 
GACACCTCTC TACTATATAA CGAGTACATT GTCTATGATA TTGCTCAGGT AAATCTGAAG 
TATCTGCTGA AACTGAAATT CAATTTTAAG ACCTCCCTGT GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PARP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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