PARP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene
Clones
gRNAs

Summary

Gene Symbol	PARP1
Entrez Gene ID	817690
Full Name	poly(ADP-ribose) polymerase 2
Synonyms	ATPARP2,F16D14.16,F16D14_16,POLY(ADP-RIBOSE) POLYMERASE 1,poly(ADP-ribose) polymerase 2
Gene Type	protein-coding
Organism	Arabidopsis thaliana(thale cress)

mRNA and Protein(s)

mRNA	Protein	Name
NM_179834.2	NP_850165.1	poly(ADP-ribose) polymerase 2

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Parp	NP_001104452.1
Arabidopsis thaliana (thale cress)	PARP1	NP_850165.1
Oryza sativa (rice)	Os07g0413700	NP_001059453.1
Homo sapiens (human)	PARP1	NP_001609.2
Pan troglodytes (chimpanzee)	PARP1	XP_003949754.1
Canis lupus familiaris (dog)	PARP1	XP_547506.3
Bos taurus (cattle)	PARP1	NP_777176.1
	PARP1	XP_001090984.1
Gallus gallus (chicken)	PARP1	NP_990594.1
Xenopus tropicalis (tropical clawed frog)	parp1	XP_002941174.2
Danio rerio (zebrafish)	parp1	NP_001038407.1
Caenorhabditis elegans (roundworm)	pme-1	NP_491072.1
Mus musculus (house mouse)	Parp1	NP_031441.2
Rattus norvegicus (Norway rat)	Parp1	NP_037195.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003230	XP_312938.4

Gene Ontology
Bibliography

Related articles in PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Lin X, Kaul S, Rounsley S, Shea TP, Benito MI, Town CD, Fujii CY, Mason T, Bowman CL, Barnstead M, Feldblyum TV, Buell CR, Ketchum KA, Lee J, Ronning CM, Koo HL, Moffat KS, Cronin LA, Shen M, Pai G, Van Aken S, Umayam L, Tallon LJ, Gill JE, Adams MD, Carrera AJ, Creasy TH, Goodman HM, Somerville CR, Copenhaver GP, Preuss D, Nierman WC, White O, Eisen JA, Salzberg SL, Fraser CM, Venter JC
Nature402(6763)761-8(1999 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We found that larger deletions of >20 bp predominated after DSB repair in ku80 and ku80 parp1 parp2 mutants, corroborating with a role of KU in preventing DSB end resection. Deletion lengths did not significantly differ between ku80 and ku80 parp1 parp2 mutants, suggesting that a KU- and PARP-independent b-NHEJ mechanism becomes active in these mutants.
Title: CRISPR/Cas9-Induced Double-Strand Break Repair in Arabidopsis Nonhomologous End-Joining Mutants.

whilst all isoforms of PARP were localized to the nucleus they are also present in non-nuclear locations with parp1 and parp3 also localised in the cytosol, and parp2 also present in the mitochondria
Title: Analysis of knockout mutants reveals non-redundant functions of poly(ADP-ribose)polymerase isoforms in Arabidopsis.

Studies indicate that a massive and rapid accumulation of a massive and rapid accumulation poly(ADP-ribose) polymerases AtPARP1 and AtPARP2 transcripts was observed upon treatment with ionizing radiation and reactive oxigen species (ROS).
Title: Synthesis and degradation of poly(ADP-ribose) in plants.

PARP isoforms play a minor role in telomere length maintenance in Arabidopsis.
Title: Analysis of poly(ADP-Ribose) polymerases in Arabidopsis telomere biology.

Evidence suggests a link between the glutathione pool and PARP expression and activity that is perhaps related to the distribution of intracellular glutathione between the cytoplasm and the nucleus. [PARP1]
Title: Pyridine nucleotide cycling and control of intracellular redox state in relation to poly (ADP-ribose) polymerase activity and nuclear localization of glutathione during exponential growth of Arabidopsis cells in culture.

The following PARP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PARP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAb08444	NM_179834.2 Latest version!	Arabidopsis thaliana poly(ADP-ribose) polymerase 2 (PARP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PARP1 ( NM_179834.2 ) cDNA ORF clone, Arabidopsis thaliana(thale cress) -> NP_850165.1 Arabidopsis thaliana poly(ADP-ribose) polymerase 2 (PARP1), mRNA.

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CloneID	OAb08444
Clone ID Related Accession (Same CDS sequence)	NM_179834.2
Accession Version	NM_179834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-13
Organism	Arabidopsis thaliana(thale cress)
Product	poly(ADP-ribose) polymerase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by TAIR and Araport. This record is derived from an annotated genomic sequence (NC_003071). On Sep 12, 2016 this sequence version replaced NM_179834.1.

ATGGCAAGCC CACATAAGCC GTGGAGGGCG GAGTATGCAA AGTCGTCGAG GTCTTCATGT 
AAAACTTGCA AGTCCGTCAT TAACAAGGAG AACTTTCGTC TTGGAAAGTT GGTTCAATCT 
ACTCACTTCG ATGGCATCAT GCCCATGTGG AACCATGCTT CTTGTATACT AAAGAAGACG 
AAGCAGATAA AATCAGTTGA TGATGTTGAA GGCATAGAAT CACTTCGTTG GGAAGATCAG 
CAAAAGATTA GAAAATATGT CGAATCTGGA GCAGGGAGTA ACACAAGCAC AAGCACAGGC 
ACAAGCACGA GCAGTACCGC TAATAATGCC AAACTAGAAT ATGGGATTGA AGTGTCACAA 
ACTTCTCGTG CCGGTTGCAG AAAGTGTAGC GAAAAGATCT TGAAAGGAGA GGTACGTATA 
TTCTCCAAGC CTGAAGGCCC GGGTAACAAA GGTTTGATGT GGCATCACGC TAAATGTTTC 
CTTGAAATGT CTTCCTCTAC TGAACTGGAA AGTTTGTCTG GATGGAGAAG TATACCAGAC 
TCAGACCAAG AAGCTCTTCT TCCCTTAGTG AAGAAAGCTC TGCCGGCAGC CAAAACTGAG 
ACAGCAGAAG CACGTCAAAC AAATTCAAGA GCAGGCACAA AACGAAAAAA TGATTCTGTT 
GATAACGAGA AGTCGAAACT AGCAAAAAGT AGTTTTGACA TGTCTACAAG TGGTGCTTTA 
CAACCTTGTA GCAAAGAAAA GGAAATGGAG GCCCAAACTA AGGAATTGTG GGACCTGAAG 
GATGATCTGA AAAAATATGT AACATCAGCT GAGTTGCGGG AAATGCTTGA AGTAAATGAA 
CAAAGTACAA GAGGATCTGA ACTTGATCTG CGTGATAAAT GTGCTGATGG CATGATGTTT 
GGCCCACTCG CTCTCTGCCC AATGTGCTCT GGGCATCTTT CTTTCTCCGG AGGACTTTAC 
CGATGCCATG GATACATCTC AGAATGGAGC AAATGTTCTC ATTCCACTTT GGATCCAGAC 
CGCATCAAAG GGAAGTGGAA AATCCCTGAC GAAACAGAAA ATCAATTCCT TCTGAAGTGG 
AATAAGTCTC AAAAGAGTGT GAAGCCAAAA CGTATTCTGC GTCCTGTATT GTCTGGCGAG 
ACATCTCAGG GTCAAGGTTC TAAAGATGCA ACTGACTCCT CAAGGAGTGA AAGGCTAGCA 
GATCTTAAAG TTTCAATTGC TGGAAATACT AAGGAAAGGC AACCATGGAA GAAGAGAATT 
GAGGAAGCTG GTGCAGAGTT TCATGCTAAT GTTAAAAAAG GTACAAGCTG TTTGGTTGTT 
TGTGGACTGA CAGATATCAG AGACGCTGAA ATGAGAAAGG CAAGGAGGAT GAAAGTGGCA 
ATCGTGAGAG AGGATTATTT GGTTGATTGT TTTAAAAAAC AGAGGAAACT TCCATTTGAC 
AAGTACAAAA TTGAAGACAC TAGTGAGAGC CTTGTCACTG TTAAAGTAAA AGGACGAAGC 
GCTGTGCATG AAGCGTCTGG CCTCCAAGAG CACTGTCACA TTCTTGAAGA TGGGAACAGT 
ATCTATAACA CAACTCTGAG CATGTCTGAT CTCTCTACCG GTATCAATAG TTATTACATA 
CTCCAGATAA TCCAAGAAGA TAAAGGTTCA GATTGTTACG TATTTCGTAA ATGGGGCCGA 
GTTGGAAATG AAAAGATTGG TGGTAACAAA GTGGAGGAAA TGTCAAAGTC TGATGCGGTT 
CACGAATTCA AACGTCTATT TCTTGAAAAA ACCGGAAACA CATGGGAATC TTGGGAACAA 
AAAACGAATT TCCAGAAACA ACCTGGAAAA TTTCTCCCGT TGGACATTGA TTATGGAGTT 
AATAAGCAAG TAGCCAAAAA AGAGCCATTT CAGACCAGTA GCAACCTTGC TCCATCATTA 
ATAGAATTGA TGAAGATGCT TTTTGATGTG GAAACTTACA GATCTGCAAT GATGGAGTTC 
GAGATAAATA TGTCAGAGAT GCCACTTGGG AAGCTCAGCA AACATAATAT ACAGAAGGGT 
TTTGAGGCAT TGACGGAGAT ACAGAGGCTA TTGACTGAAA GCGACCCCCA GCCTACTATG 
AAAGAAAGCT TGCTTGTTGA TGCTAGTAAC AGATTTTTTA CCATGATCCC TTCTATTCAT 
CCTCATATTA TCCGAGATGA AGATGACTTT AAGTCAAAGG TGAAAATGCT CGAGGCTCTG 
CAGGATATCG AAATAGCTTC AAGAATAGTT GGCTTTGATG TTGATAGCAC CGAATCTCTT 
GATGATAAGT ATAAGAAGCT GCATTGCGAT ATCTCACCAC TTCCTCATGA TAGCGAAGAT 
TATCGATTAA TCGAGAAGTA TCTTAACACA ACTCATGCCC CAACGCATAC AGAGTGGAGT 
CTTGAGCTAG AGGAAGTTTT TGCTCTTGAA AGAGAAGGAG AGTTTGATAA ATATGCTCCC 
CACAGAGAAA AACTTGGCAA TAAGATGCTC CTATGGCATG GTTCTCGATT AACGAATTTT 
GTTGGAATAT TGAACCAAGG ACTGAGAATT GCACCTCCAG AAGCTCCTGC TACTGGTTAC 
ATGTTTGGAA AAGGGATATA CTTTGCTGAC CTTGTCAGTA AAAGTGCTCA GTACTGCTAC 
ACTTGTAAGA AAAATCCGGT GGGTCTAATG CTTCTGAGTG AAGTTGCATT GGGAGAAATA 
CATGAGCTAA CAAAAGCTAA GTATATGGAT AAACCTCCGA GAGGGAAACA CTCGACCAAA 
GGGCTCGGCA AGAAAGTGCC TCAAGATTCC GAGTTTGCCA AGTGGAGAGG TGATGTGACT 
GTTCCCTGTG GAAAACCTGT TTCATCAAAG GTCAAGGCTT CTGAGCTTAT GTACAATGAG 
TATATCGTCT ACGATACAGC CCAGGTGAAG TTGCAGTTCT TGTTGAAAGT AAGGTTTAAG 
CACAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_850165.1
CDS	173..3124
Translation	MASPHKPWRAEYAKSSRSSCKTCKSVINKENFRLGKLVQSTHFDGIMPMWNHASCILKKTKQIKSVDDVEGIESLRWEDQQKIRKYVESGAGSNTSTSTGTSTSSTANNAKLEYGIEVSQTSRAGCRKCSEKILKGEVRIFSKPEGPGNKGLMWHHAKCFLEMSSSTELESLSGWRSIPDSDQEALLPLVKKALPAAKTETAEARQTNSRAGTKRKNDSVDNEKSKLAKSSFDMSTSGALQPCSKEKEMEAQTKELWDLKDDLKKYVTSAELREMLEVNEQSTRGSELDLRDKCADGMMFGPLALCPMCSGHLSFSGGLYRCHGYISEWSKCSHSTLDPDRIKGKWKIPDETENQFLLKWNKSQKSVKPKRILRPVLSGETSQGQGSKDATDSSRSERLADLKVSIAGNTKERQPWKKRIEEAGAEFHANVKKGTSCLVVCGLTDIRDAEMRKARRMKVAIVREDYLVDCFKKQRKLPFDKYKIEDTSESLVTVKVKGRSAVHEASGLQEHCHILEDGNSIYNTTLSMSDLSTGINSYYILQIIQEDKGSDCYVFRKWGRVGNEKIGGNKVEEMSKSDAVHEFKRLFLEKTGNTWESWEQKTNFQKQPGKFLPLDIDYGVNKQVAKKEPFQTSSNLAPSLIELMKMLFDVETYRSAMMEFEINMSEMPLGKLSKHNIQKGFEALTEIQRLLTESDPQPTMKESLLVDASNRFFTMIPSIHPHIIRDEDDFKSKVKMLEALQDIEIASRIVGFDVDSTESLDDKYKKLHCDISPLPHDSEDYRLIEKYLNTTHAPTHTEWSLELEEVFALEREGEFDKYAPHREKLGNKMLLWHGSRLTNFVGILNQGLRIAPPEAPATGYMFGKGIYFADLVSKSAQYCYTCKKNPVGLMLLSEVALGEIHELTKAKYMDKPPRGKHSTKGLGKKVPQDSEFAKWRGDVTVPCGKPVSSKVKASELMYNEYIVYDTAQVKLQFLLKVRFKHKR

Target ORF information:

RefSeq Version	NM_179834.2
Organism	Arabidopsis thaliana(thale cress)
Definition	Arabidopsis thaliana poly(ADP-ribose) polymerase 2 (PARP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_179834.2

ORF Insert Sequence:

ATGGCAAGCC CACATAAGCC GTGGAGGGCG GAGTATGCAA AGTCGTCGAG GTCTTCATGT 
AAAACTTGCA AGTCCGTCAT TAACAAGGAG AACTTTCGTC TTGGAAAGTT GGTTCAATCT 
ACTCACTTCG ATGGCATCAT GCCCATGTGG AACCATGCTT CTTGTATACT AAAGAAGACG 
AAGCAGATAA AATCAGTTGA TGATGTTGAA GGCATAGAAT CACTTCGTTG GGAAGATCAG 
CAAAAGATTA GAAAATATGT CGAATCTGGA GCAGGGAGTA ACACAAGCAC AAGCACAGGC 
ACAAGCACGA GCAGTACCGC TAATAATGCC AAACTAGAAT ATGGGATTGA AGTGTCACAA 
ACTTCTCGTG CCGGTTGCAG AAAGTGTAGC GAAAAGATCT TGAAAGGAGA GGTACGTATA 
TTCTCCAAGC CTGAAGGCCC GGGTAACAAA GGTTTGATGT GGCATCACGC TAAATGTTTC 
CTTGAAATGT CTTCCTCTAC TGAACTGGAA AGTTTGTCTG GATGGAGAAG TATACCAGAC 
TCAGACCAAG AAGCTCTTCT TCCCTTAGTG AAGAAAGCTC TGCCGGCAGC CAAAACTGAG 
ACAGCAGAAG CACGTCAAAC AAATTCAAGA GCAGGCACAA AACGAAAAAA TGATTCTGTT 
GATAACGAGA AGTCGAAACT AGCAAAAAGT AGTTTTGACA TGTCTACAAG TGGTGCTTTA 
CAACCTTGTA GCAAAGAAAA GGAAATGGAG GCCCAAACTA AGGAATTGTG GGACCTGAAG 
GATGATCTGA AAAAATATGT AACATCAGCT GAGTTGCGGG AAATGCTTGA AGTAAATGAA 
CAAAGTACAA GAGGATCTGA ACTTGATCTG CGTGATAAAT GTGCTGATGG CATGATGTTT 
GGCCCACTCG CTCTCTGCCC AATGTGCTCT GGGCATCTTT CTTTCTCCGG AGGACTTTAC 
CGATGCCATG GATACATCTC AGAATGGAGC AAATGTTCTC ATTCCACTTT GGATCCAGAC 
CGCATCAAAG GGAAGTGGAA AATCCCTGAC GAAACAGAAA ATCAATTCCT TCTGAAGTGG 
AATAAGTCTC AAAAGAGTGT GAAGCCAAAA CGTATTCTGC GTCCTGTATT GTCTGGCGAG 
ACATCTCAGG GTCAAGGTTC TAAAGATGCA ACTGACTCCT CAAGGAGTGA AAGGCTAGCA 
GATCTTAAAG TTTCAATTGC TGGAAATACT AAGGAAAGGC AACCATGGAA GAAGAGAATT 
GAGGAAGCTG GTGCAGAGTT TCATGCTAAT GTTAAAAAAG GTACAAGCTG TTTGGTTGTT 
TGTGGACTGA CAGATATCAG AGACGCTGAA ATGAGAAAGG CAAGGAGGAT GAAAGTGGCA 
ATCGTGAGAG AGGATTATTT GGTTGATTGT TTTAAAAAAC AGAGGAAACT TCCATTTGAC 
AAGTACAAAA TTGAAGACAC TAGTGAGAGC CTTGTCACTG TTAAAGTAAA AGGACGAAGC 
GCTGTGCATG AAGCGTCTGG CCTCCAAGAG CACTGTCACA TTCTTGAAGA TGGGAACAGT 
ATCTATAACA CAACTCTGAG CATGTCTGAT CTCTCTACCG GTATCAATAG TTATTACATA 
CTCCAGATAA TCCAAGAAGA TAAAGGTTCA GATTGTTACG TATTTCGTAA ATGGGGCCGA 
GTTGGAAATG AAAAGATTGG TGGTAACAAA GTGGAGGAAA TGTCAAAGTC TGATGCGGTT 
CACGAATTCA AACGTCTATT TCTTGAAAAA ACCGGAAACA CATGGGAATC TTGGGAACAA 
AAAACGAATT TCCAGAAACA ACCTGGAAAA TTTCTCCCGT TGGACATTGA TTATGGAGTT 
AATAAGCAAG TAGCCAAAAA AGAGCCATTT CAGACCAGTA GCAACCTTGC TCCATCATTA 
ATAGAATTGA TGAAGATGCT TTTTGATGTG GAAACTTACA GATCTGCAAT GATGGAGTTC 
GAGATAAATA TGTCAGAGAT GCCACTTGGG AAGCTCAGCA AACATAATAT ACAGAAGGGT 
TTTGAGGCAT TGACGGAGAT ACAGAGGCTA TTGACTGAAA GCGACCCCCA GCCTACTATG 
AAAGAAAGCT TGCTTGTTGA TGCTAGTAAC AGATTTTTTA CCATGATCCC TTCTATTCAT 
CCTCATATTA TCCGAGATGA AGATGACTTT AAGTCAAAGG TGAAAATGCT CGAGGCTCTG 
CAGGATATCG AAATAGCTTC AAGAATAGTT GGCTTTGATG TTGATAGCAC CGAATCTCTT 
GATGATAAGT ATAAGAAGCT GCATTGCGAT ATCTCACCAC TTCCTCATGA TAGCGAAGAT 
TATCGATTAA TCGAGAAGTA TCTTAACACA ACTCATGCCC CAACGCATAC AGAGTGGAGT 
CTTGAGCTAG AGGAAGTTTT TGCTCTTGAA AGAGAAGGAG AGTTTGATAA ATATGCTCCC 
CACAGAGAAA AACTTGGCAA TAAGATGCTC CTATGGCATG GTTCTCGATT AACGAATTTT 
GTTGGAATAT TGAACCAAGG ACTGAGAATT GCACCTCCAG AAGCTCCTGC TACTGGTTAC 
ATGTTTGGAA AAGGGATATA CTTTGCTGAC CTTGTCAGTA AAAGTGCTCA GTACTGCTAC 
ACTTGTAAGA AAAATCCGGT GGGTCTAATG CTTCTGAGTG AAGTTGCATT GGGAGAAATA 
CATGAGCTAA CAAAAGCTAA GTATATGGAT AAACCTCCGA GAGGGAAACA CTCGACCAAA 
GGGCTCGGCA AGAAAGTGCC TCAAGATTCC GAGTTTGCCA AGTGGAGAGG TGATGTGACT 
GTTCCCTGTG GAAAACCTGT TTCATCAAAG GTCAAGGCTT CTGAGCTTAT GTACAATGAG 
TATATCGTCT ACGATACAGC CCAGGTGAAG TTGCAGTTCT TGTTGAAAGT AAGGTTTAAG 
CACAAGAGAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.
Nature402(6763)761-8(1999 Dec)
Lin X,Kaul S,Rounsley S,Shea TP,Benito MI,Town CD,Fujii CY,Mason T,Bowman CL,Barnstead M,Feldblyum TV,Buell CR,Ketchum KA,Lee J,Ronning CM,Koo HL,Moffat KS,Cronin LA,Shen M,Pai G,Van Aken S,Umayam L,Tallon LJ,Gill JE,Adams MD,Carrera AJ,Creasy TH,Goodman HM,Somerville CR,Copenhaver GP,Preuss D,Nierman WC,White O,Eisen JA,Salzberg SL,Fraser CM,Venter JC

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PARP1 cDNA ORF clone, Arabidopsis thaliana(thale cress)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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