PARP1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PARP1
Entrez Gene ID	286764
Full Name	poly(ADP-ribose) polymerase 1
Synonyms	ADPRT,ARTD1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005216796.3	XP_005216853.1	poly [ADP-ribose] polymerase 1 isoform X1
XM_005216796.4	XP_005216853.1	poly [ADP-ribose] polymerase 1 isoform X1
NM_174751.2	NP_777176.1	poly [ADP-ribose] polymerase 1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Parp	NP_001104452.1
Arabidopsis thaliana (thale cress)	PARP1	NP_850165.1
Oryza sativa (rice)	Os07g0413700	NP_001059453.1
Homo sapiens (human)	PARP1	NP_001609.2
Pan troglodytes (chimpanzee)	PARP1	XP_003949754.1
Canis lupus familiaris (dog)	PARP1	XP_547506.3
Bos taurus (cattle)	PARP1	NP_777176.1
	PARP1	XP_001090984.1
Gallus gallus (chicken)	PARP1	NP_990594.1
Xenopus tropicalis (tropical clawed frog)	parp1	XP_002941174.2
Danio rerio (zebrafish)	parp1	NP_001038407.1
Caenorhabditis elegans (roundworm)	pme-1	NP_491072.1
Mus musculus (house mouse)	Parp1	NP_031441.2
Rattus norvegicus (Norway rat)	Parp1	NP_037195.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003230	XP_312938.4

Gene Ontology
Bibliography

Related articles in PubMed

Assessment of calpain and caspase systems activities during ageing of two bovine muscles by degradation patterns of ?II spectrin and PARP-1.
Sacc? E, Pizzutti N, Corazzin M, Lippe G, Piasentier E
Animal science journal = Nihon chikusan Gakkaiho87(3)462-6(2016 Mar)

Lack of an association between two BER gene polymorphisms and breast cancer risk: a meta-analysis.
Wu B, Liu HL, Zhang S, Dong XR, Wu G
PloS one7(12)e50857(2012)

Poly(ADP-ribose) polymerase 1 interaction with apurinic/apyrimidinic sites.
Khodyreva SN, Ilina ES, Kutuzov MM, Sukhanova MV, Lavrik OI
Doklady. Biochemistry and biophysics43169-72(2010 Mar-Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.
Caldini R, Barletta E, Del Rosso M, Giovannelli L, Chevanne M
Journal of cellular physiology202(1)125-34(2005 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

no difference was found in the level of SBDP145 between muscles, while SBDP120 and PARP-1 cleavage products were not detected
Title: Assessment of calpain and caspase systems activities during ageing of two bovine muscles by degradation patterns of ?II spectrin and PARP-1.

ADPRT Val762Ala and APE1 Asp148Glu polymorphisms are not associated with increased breast cancer risk
Title: Lack of an association between two BER gene polymorphisms and breast cancer risk: a meta-analysis.

analysis of poly(ADP-ribose) polymerase 1 interaction with apurinic/apyrimidinic sites
Title: Poly(ADP-ribose) polymerase 1 interaction with apurinic/apyrimidinic sites.

FGF2 stimulates poly(ADP-ribose) polymerase activity by a DNA strand breaks-independent manner which involves a mitogen-activated protein kinases (MAPK)-dependent pathway
Title: FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.

The following PARP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PARP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41307	NM_174751.2 Latest version!	Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa27066	XM_005216796.4 Latest version!	Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa27066	XM_005216796.3	Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PARP1 ( NM_174751.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777176.1 Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), mRNA.

Price & Availability↑

CloneID	OBa41307
Clone ID Related Accession (Same CDS sequence)	NM_174751.2
Accession Version	NM_174751.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3051bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from D90073.1. On Jun 3, 2003 this sequence version replaced NM_174751.1. ##Evidence-Data-START## Transcript exon combination :: D90073.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGAGT CTTCAGACAA GCTCTACCGG GTCGAGTACG CCAAGAGCGG GCGCGCGTCT 
TGCAAGAAAT GCAAAGAGAG CATCCCCAAG GACTCGATCC GGATGGCCTT CATGGTGGAG 
TCGCCCATGT TCGATGGGAA AATCCCGCAC TGGTACCACC TCTCCTGCTT CTGGAAGGTC 
GGCTTCTCCA TCTGGCACCC TGATGTCGAG GTGGAGGGGT TCTCTGAGCT CCGCTGGGAT 
GACCAGCAGA CGATCAAGAA GATGGCCGAG ACTGGCGGAC GGACAGATGT TTCAGGCAAA 
GGCCAAGATG GAGTTGGCAG CAAGACCGAG AAGACGTTGA TTGACTTCGG GGCAGGGTAC 
GCCAAGTCCA ACAGAAGCAC GTGCAAGAGC TGCATGGAGA AGATAGACAA GGGCCAGGTG 
CGCCTGTCTA AGAAGGTGGT GTACCCCGAT AAGCCCCAGC TGGGCATGGT TGACTGCTGG 
TACCACCCAA AGTGTTTTGT TCAGAAACGG GAGGAGCTGG GCTTCCGTCC CGAGTTCAGC 
GCAACGCACC TCATGGGCTT CAGCGTCCTC ACCGCAGAGG ACCAAGAAAC CCTCAAGAAG 
CAACTCCCGG CCATCAAGGG TGAAAGAAAG AGAAAAGGTG ATGAGGTGGA TGGAATAGAT 
GAAGTGACCA AGAAGAAGTC TAAAAAAGAA AAAGACAAGG AGATTAAACT TGAAAAGGCC 
CTTAAGGCCC AGAACGACCT GATCTGGAAT GTCAAGGACG AGCTAAAGAA AGCGTGCTCT 
ACGAACGACC TGAAAGAGTT GCTCATCTTC AACAAGCAGG AAGTGCCTTC CGGGGAGTCG 
GCGATCTTGG ACCGCGTGGC CGACGGTATG GTGTTTGGCG CCCTCCTTCC CTGCGAGGAA 
TGCTCGGGCC AGCTGGTCTT CAAGGGCGAC GCCTATTACT GTACCGGGGA TGTGACTGCC 
TGGACCAAGT GTATGGTCAA GACACAGACG CCCAACCGGA AGGAGTGGGT GACCCCAAAG 
GAATTCCGAG AAATCTCTTA CTTCAAGAAA CTGAAGATCA AAAAGCAGGA CCGTATATTC 
CCCCCAGAGA GCAGCACCCC AGTGGGGGCA GCAGCCCCAC CCTCCGCAGC TTCAGCGCCT 
GCCGCTGTGC ACTCTGGCCC CCCAGACAAG CCATTATCCA ACATGAAGAT CCTGACTCTC 
GGGAAACTCT CCCAGAACAA GGATGAAGTG AAGGCCACGA TTGAGAAACT CGGGGGGAAA 
TTGACAGGGA CGGCCAACAA GGCCTCCCTG TGTATCAGCA CCAAAAAGGA GGTGGACAAG 
TTGAATAAAA AGATGGAGGA AGTAAAAGAA GCCAACATCC GTGTCGTGTC TGAGGACTTC 
CTCCAAGACA TCTCCGCCTC CACCAAGAGC CTTCAGGAGT TGCTCTCCAC CCACCTCTTG 
TCCCCCTGGG GAGCCGAGGT GAAGGTGGAG CCTGTTGAAG CAGTGGGCCC AAAGGGGAAG 
TCGGGGGCCG CGCCCTCCAA GAAGAGCAAG GGTCCCGTCA AGGAGGAAGG TACCAACAAA 
TCTGAAAAGA GGATGAAATT AACTCTTAAA GGAGGAGCAG CTGTCGACCC TGATTCAGGT 
CTGGAACACA ATGCACACGT CCTCGAGAAA GGCGGGAAGG TCTTCAGCGC CACCCTCGGG 
CTCGTGGACA TCGTCAAAGG GACCAACTCC TATTACAAGC TGCAGCTCCT GGAGGATGAC 
AAAGAGAGCA GGTACTGGAT ATTCAGGTCC TGGGGCCGTG TGGGCACGGT GATTGGTAGT 
AACAAACTGG AGCAGATGCC ATCCAAGGAG GATGCCATTG AGCATTTTAT GAAATTATAT 
GAAGAGAAAA CCGGAAACGC CTGGCACTCT AAAAACTTTA CGAAGCATCC CAAAAAGTTC 
TACCCTCTGG AGATTGACTA CGGCCAGGAT GAAGAGGCGG TGAAGAAGTT GACAGTAAAC 
CCTGGCACCA AGTCCAAGCT CCCCAAGCCA GTGCAGAACC TCATTAAGAT GATCTTTGAT 
GTAGAGAGTA TGAAGAAAGC CATGGTGGAG TATGAGATTG ACCTTCAGAA GATGCCCTTG 
GGGAAGCTGA GCAAAAGGCA GATCCAGGCT GCATACTCCA TCCTCAGTGA GGTCCAGCAG 
GCACTGTCCC AGGGCAGCAG TGACTCTCAC ATCCTGGATC TCTCCAACCG CTTCTACACC 
CTGATCCCCC ACGACTTCGG GATGAAGAAG CCCCCGCTGC TGAACAACGC AAACAGCGTG 
CAGGCCAAGG TGGAAATGCT AGACAACCTG CTGGATATTG AGGTGGCCTA CAGTCTACTT 
AGGGGTGGTT CTGATGACAG CAGCAAGGAC CCCATTGATG TCAACTATGA GAAGCTCAAA 
ACTGACATTA AGGTTGTGGA CAAAGATTCC GAAGAAGCCG AGATCATTAG GAAGTATGTG 
AAGAACACTC ACGCGACCAC ACACAACGCG TACGACTTAG AAGTCGTCGA CATCTTCAAG 
ATAGAGCGCG AAGGGGAAAG CCAGCGTTAC AAGCCGTTTA AGCAGCTGCA TAACCGGAGG 
CTGCTGTGGC ACGGGTCCAG GACCACCAAC TTCGCGGGCA TCCTGTCCCA GGGTCTCCGG 
ATAGCCCCAC CTGAAGCACC TGTGACGGGC TACATGTTTG GTAAGGGAAT CTATTTCGCG 
GACATGGTCT CCAAGAGTGC CAACTACTGC CACACGTCCC AGGGAGACCC AATAGGCTTG 
ATCCTGTTGG GAGAAGCTGC CCTTGGAAAC ATGTATGAAT TGAAACATGC TCGACATATC 
AGCAAGTTAC CCAAGGGCAA GCACAGTGTC AAAGGTTTAG GCAAAACTAC CCCTGACCCA 
TCGGCTAGTA TTACTGTGGA TGGTGTGGAG GTGCCTCTCG GGACAGGGAT TTCATCTGGT 
GTTAATGACA CCTGTCTGTT GTATAACGAG TACATCGTCT ACGACATTGC TCAGGTCCAC 
CTGAAGTACC TGCTGAAGCT GAAGTTCAAC TTTAAGACAT CCCTGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777176.1
CDS	77..3127
Translation	MAESSDKLYRVEYAKSGRASCKKCKESIPKDSIRMAFMVESPMFDGKIPHWYHLSCFWKVGFSIWHPDVEVEGFSELRWDDQQTIKKMAETGGRTDVSGKGQDGVGSKTEKTLIDFGAGYAKSNRSTCKSCMEKIDKGQVRLSKKVVYPDKPQLGMVDCWYHPKCFVQKREELGFRPEFSATHLMGFSVLTAEDQETLKKQLPAIKGERKRKGDEVDGIDEVTKKKSKKEKDKEIKLEKALKAQNDLIWNVKDELKKACSTNDLKELLIFNKQEVPSGESAILDRVADGMVFGALLPCEECSGQLVFKGDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYFKKLKIKKQDRIFPPESSTPVGAAAPPSAASAPAAVHSGPPDKPLSNMKILTLGKLSQNKDEVKATIEKLGGKLTGTANKASLCISTKKEVDKLNKKMEEVKEANIRVVSEDFLQDISASTKSLQELLSTHLLSPWGAEVKVEPVEAVGPKGKSGAAPSKKSKGPVKEEGTNKSEKRMKLTLKGGAAVDPDSGLEHNAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKESRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKHPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQNLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQALSQGSSDSHILDLSNRFYTLIPHDFGMKKPPLLNNANSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDKDSEEAEIIRKYVKNTHATTHNAYDLEVVDIFKIEREGESQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEAALGNMYELKHARHISKLPKGKHSVKGLGKTTPDPSASITVDGVEVPLGTGISSGVNDTCLLYNEYIVYDIAQVHLKYLLKLKFNFKTSLW

Target ORF information:

RefSeq Version	NM_174751.2
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174751.2

ORF Insert Sequence:

ATGGCGGAGT CTTCAGACAA GCTCTACCGG GTCGAGTACG CCAAGAGCGG GCGCGCGTCT 
TGCAAGAAAT GCAAAGAGAG CATCCCCAAG GACTCGATCC GGATGGCCTT CATGGTGGAG 
TCGCCCATGT TCGATGGGAA AATCCCGCAC TGGTACCACC TCTCCTGCTT CTGGAAGGTC 
GGCTTCTCCA TCTGGCACCC TGATGTCGAG GTGGAGGGGT TCTCTGAGCT CCGCTGGGAT 
GACCAGCAGA CGATCAAGAA GATGGCCGAG ACTGGCGGAC GGACAGATGT TTCAGGCAAA 
GGCCAAGATG GAGTTGGCAG CAAGACCGAG AAGACGTTGA TTGACTTCGG GGCAGGGTAC 
GCCAAGTCCA ACAGAAGCAC GTGCAAGAGC TGCATGGAGA AGATAGACAA GGGCCAGGTG 
CGCCTGTCTA AGAAGGTGGT GTACCCCGAT AAGCCCCAGC TGGGCATGGT TGACTGCTGG 
TACCACCCAA AGTGTTTTGT TCAGAAACGG GAGGAGCTGG GCTTCCGTCC CGAGTTCAGC 
GCAACGCACC TCATGGGCTT CAGCGTCCTC ACCGCAGAGG ACCAAGAAAC CCTCAAGAAG 
CAACTCCCGG CCATCAAGGG TGAAAGAAAG AGAAAAGGTG ATGAGGTGGA TGGAATAGAT 
GAAGTGACCA AGAAGAAGTC TAAAAAAGAA AAAGACAAGG AGATTAAACT TGAAAAGGCC 
CTTAAGGCCC AGAACGACCT GATCTGGAAT GTCAAGGACG AGCTAAAGAA AGCGTGCTCT 
ACGAACGACC TGAAAGAGTT GCTCATCTTC AACAAGCAGG AAGTGCCTTC CGGGGAGTCG 
GCGATCTTGG ACCGCGTGGC CGACGGTATG GTGTTTGGCG CCCTCCTTCC CTGCGAGGAA 
TGCTCGGGCC AGCTGGTCTT CAAGGGCGAC GCCTATTACT GTACCGGGGA TGTGACTGCC 
TGGACCAAGT GTATGGTCAA GACACAGACG CCCAACCGGA AGGAGTGGGT GACCCCAAAG 
GAATTCCGAG AAATCTCTTA CTTCAAGAAA CTGAAGATCA AAAAGCAGGA CCGTATATTC 
CCCCCAGAGA GCAGCACCCC AGTGGGGGCA GCAGCCCCAC CCTCCGCAGC TTCAGCGCCT 
GCCGCTGTGC ACTCTGGCCC CCCAGACAAG CCATTATCCA ACATGAAGAT CCTGACTCTC 
GGGAAACTCT CCCAGAACAA GGATGAAGTG AAGGCCACGA TTGAGAAACT CGGGGGGAAA 
TTGACAGGGA CGGCCAACAA GGCCTCCCTG TGTATCAGCA CCAAAAAGGA GGTGGACAAG 
TTGAATAAAA AGATGGAGGA AGTAAAAGAA GCCAACATCC GTGTCGTGTC TGAGGACTTC 
CTCCAAGACA TCTCCGCCTC CACCAAGAGC CTTCAGGAGT TGCTCTCCAC CCACCTCTTG 
TCCCCCTGGG GAGCCGAGGT GAAGGTGGAG CCTGTTGAAG CAGTGGGCCC AAAGGGGAAG 
TCGGGGGCCG CGCCCTCCAA GAAGAGCAAG GGTCCCGTCA AGGAGGAAGG TACCAACAAA 
TCTGAAAAGA GGATGAAATT AACTCTTAAA GGAGGAGCAG CTGTCGACCC TGATTCAGGT 
CTGGAACACA ATGCACACGT CCTCGAGAAA GGCGGGAAGG TCTTCAGCGC CACCCTCGGG 
CTCGTGGACA TCGTCAAAGG GACCAACTCC TATTACAAGC TGCAGCTCCT GGAGGATGAC 
AAAGAGAGCA GGTACTGGAT ATTCAGGTCC TGGGGCCGTG TGGGCACGGT GATTGGTAGT 
AACAAACTGG AGCAGATGCC ATCCAAGGAG GATGCCATTG AGCATTTTAT GAAATTATAT 
GAAGAGAAAA CCGGAAACGC CTGGCACTCT AAAAACTTTA CGAAGCATCC CAAAAAGTTC 
TACCCTCTGG AGATTGACTA CGGCCAGGAT GAAGAGGCGG TGAAGAAGTT GACAGTAAAC 
CCTGGCACCA AGTCCAAGCT CCCCAAGCCA GTGCAGAACC TCATTAAGAT GATCTTTGAT 
GTAGAGAGTA TGAAGAAAGC CATGGTGGAG TATGAGATTG ACCTTCAGAA GATGCCCTTG 
GGGAAGCTGA GCAAAAGGCA GATCCAGGCT GCATACTCCA TCCTCAGTGA GGTCCAGCAG 
GCACTGTCCC AGGGCAGCAG TGACTCTCAC ATCCTGGATC TCTCCAACCG CTTCTACACC 
CTGATCCCCC ACGACTTCGG GATGAAGAAG CCCCCGCTGC TGAACAACGC AAACAGCGTG 
CAGGCCAAGG TGGAAATGCT AGACAACCTG CTGGATATTG AGGTGGCCTA CAGTCTACTT 
AGGGGTGGTT CTGATGACAG CAGCAAGGAC CCCATTGATG TCAACTATGA GAAGCTCAAA 
ACTGACATTA AGGTTGTGGA CAAAGATTCC GAAGAAGCCG AGATCATTAG GAAGTATGTG 
AAGAACACTC ACGCGACCAC ACACAACGCG TACGACTTAG AAGTCGTCGA CATCTTCAAG 
ATAGAGCGCG AAGGGGAAAG CCAGCGTTAC AAGCCGTTTA AGCAGCTGCA TAACCGGAGG 
CTGCTGTGGC ACGGGTCCAG GACCACCAAC TTCGCGGGCA TCCTGTCCCA GGGTCTCCGG 
ATAGCCCCAC CTGAAGCACC TGTGACGGGC TACATGTTTG GTAAGGGAAT CTATTTCGCG 
GACATGGTCT CCAAGAGTGC CAACTACTGC CACACGTCCC AGGGAGACCC AATAGGCTTG 
ATCCTGTTGG GAGAAGCTGC CCTTGGAAAC ATGTATGAAT TGAAACATGC TCGACATATC 
AGCAAGTTAC CCAAGGGCAA GCACAGTGTC AAAGGTTTAG GCAAAACTAC CCCTGACCCA 
TCGGCTAGTA TTACTGTGGA TGGTGTGGAG GTGCCTCTCG GGACAGGGAT TTCATCTGGT 
GTTAATGACA CCTGTCTGTT GTATAACGAG TACATCGTCT ACGACATTGC TCAGGTCCAC 
CTGAAGTACC TGCTGAAGCT GAAGTTCAAC TTTAAGACAT CCCTGTGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP1 ( XM_005216796.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216853.1 Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa27066
Clone ID Related Accession (Same CDS sequence)	XM_005216796.3 , XM_005216796.4
Accession Version	XM_005216796.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104436.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216796.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGT CTTCAGACAA GCTCTACCGG GTCGAGTACG CCAAGAGCGG GCGCGCGTCT 
TGCAAGAAAT GCAAAGAGAG CATCCCCAAG GACTCGATCC GGATGGCCTT CATGGTGCAG 
TCGCCCATGT TCGATGGGAA AATCCCGCAC TGGTACCACC TCTCCTGCTT CTGGAAGGTC 
GGCTTCTCCA TCTGGCACCC TGATGTCGAG GTGGAGGGGT TCTCTGAGCT CCGCTGGGAT 
GACCAGCAGA CGATCAAGAA GATGGCCGAG ACTGGCGGAG CGACAGGCAA AGGCCAAGAT 
GGAGTTGGCA GCAAGACCGA GAAGACGCTG ATTGACTTCG GGGCAGGGTA CGCCAAGTCC 
AACAGAAGCA CGTGCAAGAG CTGCATGGAG AAGATAGACA AGGGCCAGGT GCGCCTGTCT 
AAGAAGGTGG TGTACCCCGA TAAGCCCCAG CTGGGCATGG TTGACTGCTG GTACCACCCA 
AAGTGTTTTG TTCAGAAACG GGAGGAGCTG GGCTTCCGTC CCGAGTTCAG CGCAAGCCAG 
CTCATGGGCT TCAGCGTCCT CACCGCAGAG GACCAAGAAA CCCTCAAGAA GCAGCTCCCG 
GCCATCAAGG GTGAAAGAAA GAGAAAAGGT GATGAGGTGG ATGGAATAGA TGAAGTGACC 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GAGATTAAAC TTGAAAAGGC CCTTAAGGCC 
CAGAACGACC TGATCTGGAA TGTCAAGGAC GAGCTAAAGA AAGCGTGCTC TACGAACGAC 
CTGAAAGAAC TGCTCATCTT CAACAAGCAG GAAGTGCCCT CCGGGGAGTC GGCGATCTTG 
GACCGCGTGG CCGACGGTAT GGTGTTTGGC GCCCTCCTTC CCTGCGAGGA ATGCTCGGGC 
CAGCTGGTCT TCAAGGGCGA CGCCTATTAC TGTACCGGGG ATGTGACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC GCCCAACCGG AAGGAGTGGG TGACCCCAAA GGAATTCCGA 
GAAATCTCTT ACTTCAAGAA ACTGAAGATC AAAAAGCAGG ACCGTATATT CCCCCCAGAG 
AGCAGCACCC CGGTGGGGGC AGCAGCCCCA CCCTCTGCAG CTTCAGCGCC TGCCGCTGTG 
CACTCTGGCC CCCCAGACAA GCCATTATCC AACATGAAGA TCCTGACTCT CGGGAAACTC 
TCCCAGAACA AGGATGAAGT GAAGGCCACG ATTGAGAAAC TCGGGGGGAA ATTGACAGGG 
ACGGCCAACA AGGCCTCCCT GTGTATCAGC ACCAAAAAGG AGGTGGACAA GATGAATAAA 
AAGATGGAGG AAGTAAAAGA AGCCAACATC CGTGTCGTGT CTGAGGACTT CCTCCAAGAC 
ATCTCCGCCT CCACCAAGAG CCTTCAGGAG TTGCTCTCCA CCCACCTCTT GTCCCCCTGG 
GGAGCCGAGG TGAAGGTGGA GCCTGTTGAA GCAGTGGCCC CAAAGGGGAA GTCGGGGGCC 
GCGCCCTCCA AGAAGAGCAA GGGTCCCGTC AAGGAGGAAG GTACCAACAA ATCTGAAAAG 
AGGATGAAAT TAACTCTTAA AGGAGGAGCA GCTGTCGACC CTGATTCAGG TCTGGAACAC 
AATGCACACG TCCTCGAGAA AGGCGGGAAG GTCTTCAGCG CCACCCTCGG GCTTGTGGAC 
ATCGTCAAAG GGACCAACTC CTATTACAAG CTGCAGCTCC TGGAGGATGA CAAAGAGAGC 
AGGTACTGGA TATTCAGGTC CTGGGGCCGT GTGGGCACGG TGATTGGTAG TAACAAACTG 
GAGCAGATGC CATCCAAGGA GGATGCCATT GAGCATTTTA TGAAATTATA TGAAGAGAAA 
ACCGGAAACG CCTGGCACTC TAAAAACTTT ACGAAGCATC CCAAAAAGTT CTACCCTCTG 
GAGATTGACT ATGGCCAGGA TGAAGAGGCG GTGAAGAAGT TGACAGTAAA CCCTGGCACC 
AAGTCCAAGC TCCCCAAGCC AGTGCAGAAC CTCATTAAGA TGATCTTTGA TGTAGAGAGT 
ATGAAGAAAG CCATGGTGGA GTATGAGATT GACCTTCAGA AGATGCCCTT GGGGAAGCTG 
AGCAAAAGGC AGATCCAGGC TGCATACTCC ATCCTCAGTG AGGTCCAGCA GGCACTGTCC 
CAGGGCAGCA GTGACTCTCA CATCCTGGAT CTCTCCAACC GCTTCTACAC CCTGATCCCC 
CACGACTTCG GGATGAAGAA GCCCCCGCTG CTGAACAACG CAAACAGCGT GCAGGCCAAG 
GTGGAAATGC TAGACAACCT GCTGGATATT GAGGTGGCCT ACAGTCTACT CAGGGGTGGT 
TCTGATGACA GCAGCAAGGA CCCCATTGAT GTCAACTATG AGAAGCTCAA AACTGACATT 
AAGGTTGTGG ACAAAGATTC CGAAGAAGCC GAGATCATTA GGAAGTATGT GAAGAACACT 
CACGCGACCA CACACAACGC GTACGACTTA GAAGTCGTCG ACATCTTCAA GATAGAGCGC 
GAAGGGGAAA GCCAGCGTTA CAAGCCGTTT AAGCAGCTGC ATAACCGGAG GCTGCTGTGG 
CACGGGTCCA GGACCACCAA CTTCGCGGGC ATCCTGTCCC AGGGTCTCCG GATAGCCCCA 
CCTGAAGCAC CTGTGACGGG CTACATGTTT GGTAAGGGAA TCTATTTCGC CGACATGGTC 
TCCAAGAGTG CCAACTACTG CCACACGTCC CAGGGAGACC CAATAGGCTT GATCCTGTTG 
GGAGAAGTTG CCCTTGGAAA CATGTATGAA CTGAAACATG CTTCGCATAT CAGCAAGTTA 
CCCAAGGGCA AGCACAGTGT CAAAGGTTTA GGCAAAACTA CCCCTGACCC ATCGGCTAGT 
ATTACTGTGG ATGGTGTGGA GGTGCCTCTC GGGACAGGGA TTTCATCTGG TGTTAATGAC 
ACCTGTCTGC TGTATAACGA GTACATCGTC TACGACATTG CTCAGGTCCA CCTGAAGTAC 
CTGCTGAAGC TGAAGTTCAA CTTTAAGACA TCCCTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216853.1
CDS	1..3042
Translation	MAESSDKLYRVEYAKSGRASCKKCKESIPKDSIRMAFMVQSPMFDGKIPHWYHLSCFWKVGFSIWHPDVEVEGFSELRWDDQQTIKKMAETGGATGKGQDGVGSKTEKTLIDFGAGYAKSNRSTCKSCMEKIDKGQVRLSKKVVYPDKPQLGMVDCWYHPKCFVQKREELGFRPEFSASQLMGFSVLTAEDQETLKKQLPAIKGERKRKGDEVDGIDEVTKKKSKKEKDKEIKLEKALKAQNDLIWNVKDELKKACSTNDLKELLIFNKQEVPSGESAILDRVADGMVFGALLPCEECSGQLVFKGDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYFKKLKIKKQDRIFPPESSTPVGAAAPPSAASAPAAVHSGPPDKPLSNMKILTLGKLSQNKDEVKATIEKLGGKLTGTANKASLCISTKKEVDKMNKKMEEVKEANIRVVSEDFLQDISASTKSLQELLSTHLLSPWGAEVKVEPVEAVAPKGKSGAAPSKKSKGPVKEEGTNKSEKRMKLTLKGGAAVDPDSGLEHNAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKESRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKHPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQNLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQALSQGSSDSHILDLSNRFYTLIPHDFGMKKPPLLNNANSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDKDSEEAEIIRKYVKNTHATTHNAYDLEVVDIFKIEREGESQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASITVDGVEVPLGTGISSGVNDTCLLYNEYIVYDIAQVHLKYLLKLKFNFKTSLW

Target ORF information:

RefSeq Version	XM_005216796.3
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216796.3

ORF Insert Sequence:

ATGGCGGAGT CTTCAGACAA GCTCTACCGG GTCGAGTACG CCAAGAGCGG GCGCGCGTCT 
TGCAAGAAAT GCAAAGAGAG CATCCCCAAG GACTCGATCC GGATGGCCTT CATGGTGCAG 
TCGCCCATGT TCGATGGGAA AATCCCGCAC TGGTACCACC TCTCCTGCTT CTGGAAGGTC 
GGCTTCTCCA TCTGGCACCC TGATGTCGAG GTGGAGGGGT TCTCTGAGCT CCGCTGGGAT 
GACCAGCAGA CGATCAAGAA GATGGCCGAG ACTGGCGGAG CGACAGGCAA AGGCCAAGAT 
GGAGTTGGCA GCAAGACCGA GAAGACGCTG ATTGACTTCG GGGCAGGGTA CGCCAAGTCC 
AACAGAAGCA CGTGCAAGAG CTGCATGGAG AAGATAGACA AGGGCCAGGT GCGCCTGTCT 
AAGAAGGTGG TGTACCCCGA TAAGCCCCAG CTGGGCATGG TTGACTGCTG GTACCACCCA 
AAGTGTTTTG TTCAGAAACG GGAGGAGCTG GGCTTCCGTC CCGAGTTCAG CGCAAGCCAG 
CTCATGGGCT TCAGCGTCCT CACCGCAGAG GACCAAGAAA CCCTCAAGAA GCAGCTCCCG 
GCCATCAAGG GTGAAAGAAA GAGAAAAGGT GATGAGGTGG ATGGAATAGA TGAAGTGACC 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GAGATTAAAC TTGAAAAGGC CCTTAAGGCC 
CAGAACGACC TGATCTGGAA TGTCAAGGAC GAGCTAAAGA AAGCGTGCTC TACGAACGAC 
CTGAAAGAAC TGCTCATCTT CAACAAGCAG GAAGTGCCCT CCGGGGAGTC GGCGATCTTG 
GACCGCGTGG CCGACGGTAT GGTGTTTGGC GCCCTCCTTC CCTGCGAGGA ATGCTCGGGC 
CAGCTGGTCT TCAAGGGCGA CGCCTATTAC TGTACCGGGG ATGTGACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC GCCCAACCGG AAGGAGTGGG TGACCCCAAA GGAATTCCGA 
GAAATCTCTT ACTTCAAGAA ACTGAAGATC AAAAAGCAGG ACCGTATATT CCCCCCAGAG 
AGCAGCACCC CGGTGGGGGC AGCAGCCCCA CCCTCTGCAG CTTCAGCGCC TGCCGCTGTG 
CACTCTGGCC CCCCAGACAA GCCATTATCC AACATGAAGA TCCTGACTCT CGGGAAACTC 
TCCCAGAACA AGGATGAAGT GAAGGCCACG ATTGAGAAAC TCGGGGGGAA ATTGACAGGG 
ACGGCCAACA AGGCCTCCCT GTGTATCAGC ACCAAAAAGG AGGTGGACAA GATGAATAAA 
AAGATGGAGG AAGTAAAAGA AGCCAACATC CGTGTCGTGT CTGAGGACTT CCTCCAAGAC 
ATCTCCGCCT CCACCAAGAG CCTTCAGGAG TTGCTCTCCA CCCACCTCTT GTCCCCCTGG 
GGAGCCGAGG TGAAGGTGGA GCCTGTTGAA GCAGTGGCCC CAAAGGGGAA GTCGGGGGCC 
GCGCCCTCCA AGAAGAGCAA GGGTCCCGTC AAGGAGGAAG GTACCAACAA ATCTGAAAAG 
AGGATGAAAT TAACTCTTAA AGGAGGAGCA GCTGTCGACC CTGATTCAGG TCTGGAACAC 
AATGCACACG TCCTCGAGAA AGGCGGGAAG GTCTTCAGCG CCACCCTCGG GCTTGTGGAC 
ATCGTCAAAG GGACCAACTC CTATTACAAG CTGCAGCTCC TGGAGGATGA CAAAGAGAGC 
AGGTACTGGA TATTCAGGTC CTGGGGCCGT GTGGGCACGG TGATTGGTAG TAACAAACTG 
GAGCAGATGC CATCCAAGGA GGATGCCATT GAGCATTTTA TGAAATTATA TGAAGAGAAA 
ACCGGAAACG CCTGGCACTC TAAAAACTTT ACGAAGCATC CCAAAAAGTT CTACCCTCTG 
GAGATTGACT ATGGCCAGGA TGAAGAGGCG GTGAAGAAGT TGACAGTAAA CCCTGGCACC 
AAGTCCAAGC TCCCCAAGCC AGTGCAGAAC CTCATTAAGA TGATCTTTGA TGTAGAGAGT 
ATGAAGAAAG CCATGGTGGA GTATGAGATT GACCTTCAGA AGATGCCCTT GGGGAAGCTG 
AGCAAAAGGC AGATCCAGGC TGCATACTCC ATCCTCAGTG AGGTCCAGCA GGCACTGTCC 
CAGGGCAGCA GTGACTCTCA CATCCTGGAT CTCTCCAACC GCTTCTACAC CCTGATCCCC 
CACGACTTCG GGATGAAGAA GCCCCCGCTG CTGAACAACG CAAACAGCGT GCAGGCCAAG 
GTGGAAATGC TAGACAACCT GCTGGATATT GAGGTGGCCT ACAGTCTACT CAGGGGTGGT 
TCTGATGACA GCAGCAAGGA CCCCATTGAT GTCAACTATG AGAAGCTCAA AACTGACATT 
AAGGTTGTGG ACAAAGATTC CGAAGAAGCC GAGATCATTA GGAAGTATGT GAAGAACACT 
CACGCGACCA CACACAACGC GTACGACTTA GAAGTCGTCG ACATCTTCAA GATAGAGCGC 
GAAGGGGAAA GCCAGCGTTA CAAGCCGTTT AAGCAGCTGC ATAACCGGAG GCTGCTGTGG 
CACGGGTCCA GGACCACCAA CTTCGCGGGC ATCCTGTCCC AGGGTCTCCG GATAGCCCCA 
CCTGAAGCAC CTGTGACGGG CTACATGTTT GGTAAGGGAA TCTATTTCGC CGACATGGTC 
TCCAAGAGTG CCAACTACTG CCACACGTCC CAGGGAGACC CAATAGGCTT GATCCTGTTG 
GGAGAAGTTG CCCTTGGAAA CATGTATGAA CTGAAACATG CTTCGCATAT CAGCAAGTTA 
CCCAAGGGCA AGCACAGTGT CAAAGGTTTA GGCAAAACTA CCCCTGACCC ATCGGCTAGT 
ATTACTGTGG ATGGTGTGGA GGTGCCTCTC GGGACAGGGA TTTCATCTGG TGTTAATGAC 
ACCTGTCTGC TGTATAACGA GTACATCGTC TACGACATTG CTCAGGTCCA CCTGAAGTAC 
CTGCTGAAGC TGAAGTTCAA CTTTAAGACA TCCCTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP1 ( XM_005216796.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216853.1 Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), transcript variant X1, mRNA.

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CloneID	OBa27066
Clone ID Related Accession (Same CDS sequence)	XM_005216796.3 , XM_005216796.4
Accession Version	XM_005216796.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216796.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGT CTTCAGACAA GCTCTACCGG GTCGAGTACG CCAAGAGCGG GCGCGCGTCT 
TGCAAGAAAT GCAAAGAGAG CATCCCCAAG GACTCGATCC GGATGGCCTT CATGGTGCAG 
TCGCCCATGT TCGATGGGAA AATCCCGCAC TGGTACCACC TCTCCTGCTT CTGGAAGGTC 
GGCTTCTCCA TCTGGCACCC TGATGTCGAG GTGGAGGGGT TCTCTGAGCT CCGCTGGGAT 
GACCAGCAGA CGATCAAGAA GATGGCCGAG ACTGGCGGAG CGACAGGCAA AGGCCAAGAT 
GGAGTTGGCA GCAAGACCGA GAAGACGCTG ATTGACTTCG GGGCAGGGTA CGCCAAGTCC 
AACAGAAGCA CGTGCAAGAG CTGCATGGAG AAGATAGACA AGGGCCAGGT GCGCCTGTCT 
AAGAAGGTGG TGTACCCCGA TAAGCCCCAG CTGGGCATGG TTGACTGCTG GTACCACCCA 
AAGTGTTTTG TTCAGAAACG GGAGGAGCTG GGCTTCCGTC CCGAGTTCAG CGCAAGCCAG 
CTCATGGGCT TCAGCGTCCT CACCGCAGAG GACCAAGAAA CCCTCAAGAA GCAGCTCCCG 
GCCATCAAGG GTGAAAGAAA GAGAAAAGGT GATGAGGTGG ATGGAATAGA TGAAGTGACC 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GAGATTAAAC TTGAAAAGGC CCTTAAGGCC 
CAGAACGACC TGATCTGGAA TGTCAAGGAC GAGCTAAAGA AAGCGTGCTC TACGAACGAC 
CTGAAAGAAC TGCTCATCTT CAACAAGCAG GAAGTGCCCT CCGGGGAGTC GGCGATCTTG 
GACCGCGTGG CCGACGGTAT GGTGTTTGGC GCCCTCCTTC CCTGCGAGGA ATGCTCGGGC 
CAGCTGGTCT TCAAGGGCGA CGCCTATTAC TGTACCGGGG ATGTGACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC GCCCAACCGG AAGGAGTGGG TGACCCCAAA GGAATTCCGA 
GAAATCTCTT ACTTCAAGAA ACTGAAGATC AAAAAGCAGG ACCGTATATT CCCCCCAGAG 
AGCAGCACCC CGGTGGGGGC AGCAGCCCCA CCCTCTGCAG CTTCAGCGCC TGCCGCTGTG 
CACTCTGGCC CCCCAGACAA GCCATTATCC AACATGAAGA TCCTGACTCT CGGGAAACTC 
TCCCAGAACA AGGATGAAGT GAAGGCCACG ATTGAGAAAC TCGGGGGGAA ATTGACAGGG 
ACGGCCAACA AGGCCTCCCT GTGTATCAGC ACCAAAAAGG AGGTGGACAA GATGAATAAA 
AAGATGGAGG AAGTAAAAGA AGCCAACATC CGTGTCGTGT CTGAGGACTT CCTCCAAGAC 
ATCTCCGCCT CCACCAAGAG CCTTCAGGAG TTGCTCTCCA CCCACCTCTT GTCCCCCTGG 
GGAGCCGAGG TGAAGGTGGA GCCTGTTGAA GCAGTGGCCC CAAAGGGGAA GTCGGGGGCC 
GCGCCCTCCA AGAAGAGCAA GGGTCCCGTC AAGGAGGAAG GTACCAACAA ATCTGAAAAG 
AGGATGAAAT TAACTCTTAA AGGAGGAGCA GCTGTCGACC CTGATTCAGG TCTGGAACAC 
AATGCACACG TCCTCGAGAA AGGCGGGAAG GTCTTCAGCG CCACCCTCGG GCTTGTGGAC 
ATCGTCAAAG GGACCAACTC CTATTACAAG CTGCAGCTCC TGGAGGATGA CAAAGAGAGC 
AGGTACTGGA TATTCAGGTC CTGGGGCCGT GTGGGCACGG TGATTGGTAG TAACAAACTG 
GAGCAGATGC CATCCAAGGA GGATGCCATT GAGCATTTTA TGAAATTATA TGAAGAGAAA 
ACCGGAAACG CCTGGCACTC TAAAAACTTT ACGAAGCATC CCAAAAAGTT CTACCCTCTG 
GAGATTGACT ATGGCCAGGA TGAAGAGGCG GTGAAGAAGT TGACAGTAAA CCCTGGCACC 
AAGTCCAAGC TCCCCAAGCC AGTGCAGAAC CTCATTAAGA TGATCTTTGA TGTAGAGAGT 
ATGAAGAAAG CCATGGTGGA GTATGAGATT GACCTTCAGA AGATGCCCTT GGGGAAGCTG 
AGCAAAAGGC AGATCCAGGC TGCATACTCC ATCCTCAGTG AGGTCCAGCA GGCACTGTCC 
CAGGGCAGCA GTGACTCTCA CATCCTGGAT CTCTCCAACC GCTTCTACAC CCTGATCCCC 
CACGACTTCG GGATGAAGAA GCCCCCGCTG CTGAACAACG CAAACAGCGT GCAGGCCAAG 
GTGGAAATGC TAGACAACCT GCTGGATATT GAGGTGGCCT ACAGTCTACT CAGGGGTGGT 
TCTGATGACA GCAGCAAGGA CCCCATTGAT GTCAACTATG AGAAGCTCAA AACTGACATT 
AAGGTTGTGG ACAAAGATTC CGAAGAAGCC GAGATCATTA GGAAGTATGT GAAGAACACT 
CACGCGACCA CACACAACGC GTACGACTTA GAAGTCGTCG ACATCTTCAA GATAGAGCGC 
GAAGGGGAAA GCCAGCGTTA CAAGCCGTTT AAGCAGCTGC ATAACCGGAG GCTGCTGTGG 
CACGGGTCCA GGACCACCAA CTTCGCGGGC ATCCTGTCCC AGGGTCTCCG GATAGCCCCA 
CCTGAAGCAC CTGTGACGGG CTACATGTTT GGTAAGGGAA TCTATTTCGC CGACATGGTC 
TCCAAGAGTG CCAACTACTG CCACACGTCC CAGGGAGACC CAATAGGCTT GATCCTGTTG 
GGAGAAGTTG CCCTTGGAAA CATGTATGAA CTGAAACATG CTTCGCATAT CAGCAAGTTA 
CCCAAGGGCA AGCACAGTGT CAAAGGTTTA GGCAAAACTA CCCCTGACCC ATCGGCTAGT 
ATTACTGTGG ATGGTGTGGA GGTGCCTCTC GGGACAGGGA TTTCATCTGG TGTTAATGAC 
ACCTGTCTGC TGTATAACGA GTACATCGTC TACGACATTG CTCAGGTCCA CCTGAAGTAC 
CTGCTGAAGC TGAAGTTCAA CTTTAAGACA TCCCTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216853.1
CDS	176..3217
Translation	MAESSDKLYRVEYAKSGRASCKKCKESIPKDSIRMAFMVQSPMFDGKIPHWYHLSCFWKVGFSIWHPDVEVEGFSELRWDDQQTIKKMAETGGATGKGQDGVGSKTEKTLIDFGAGYAKSNRSTCKSCMEKIDKGQVRLSKKVVYPDKPQLGMVDCWYHPKCFVQKREELGFRPEFSASQLMGFSVLTAEDQETLKKQLPAIKGERKRKGDEVDGIDEVTKKKSKKEKDKEIKLEKALKAQNDLIWNVKDELKKACSTNDLKELLIFNKQEVPSGESAILDRVADGMVFGALLPCEECSGQLVFKGDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYFKKLKIKKQDRIFPPESSTPVGAAAPPSAASAPAAVHSGPPDKPLSNMKILTLGKLSQNKDEVKATIEKLGGKLTGTANKASLCISTKKEVDKMNKKMEEVKEANIRVVSEDFLQDISASTKSLQELLSTHLLSPWGAEVKVEPVEAVAPKGKSGAAPSKKSKGPVKEEGTNKSEKRMKLTLKGGAAVDPDSGLEHNAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKESRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKHPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQNLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQALSQGSSDSHILDLSNRFYTLIPHDFGMKKPPLLNNANSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDKDSEEAEIIRKYVKNTHATTHNAYDLEVVDIFKIEREGESQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASITVDGVEVPLGTGISSGVNDTCLLYNEYIVYDIAQVHLKYLLKLKFNFKTSLW

Target ORF information:

RefSeq Version	XM_005216796.4
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase 1 (PARP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216796.4

ORF Insert Sequence:

ATGGCGGAGT CTTCAGACAA GCTCTACCGG GTCGAGTACG CCAAGAGCGG GCGCGCGTCT 
TGCAAGAAAT GCAAAGAGAG CATCCCCAAG GACTCGATCC GGATGGCCTT CATGGTGCAG 
TCGCCCATGT TCGATGGGAA AATCCCGCAC TGGTACCACC TCTCCTGCTT CTGGAAGGTC 
GGCTTCTCCA TCTGGCACCC TGATGTCGAG GTGGAGGGGT TCTCTGAGCT CCGCTGGGAT 
GACCAGCAGA CGATCAAGAA GATGGCCGAG ACTGGCGGAG CGACAGGCAA AGGCCAAGAT 
GGAGTTGGCA GCAAGACCGA GAAGACGCTG ATTGACTTCG GGGCAGGGTA CGCCAAGTCC 
AACAGAAGCA CGTGCAAGAG CTGCATGGAG AAGATAGACA AGGGCCAGGT GCGCCTGTCT 
AAGAAGGTGG TGTACCCCGA TAAGCCCCAG CTGGGCATGG TTGACTGCTG GTACCACCCA 
AAGTGTTTTG TTCAGAAACG GGAGGAGCTG GGCTTCCGTC CCGAGTTCAG CGCAAGCCAG 
CTCATGGGCT TCAGCGTCCT CACCGCAGAG GACCAAGAAA CCCTCAAGAA GCAGCTCCCG 
GCCATCAAGG GTGAAAGAAA GAGAAAAGGT GATGAGGTGG ATGGAATAGA TGAAGTGACC 
AAGAAGAAAT CTAAAAAAGA AAAAGACAAG GAGATTAAAC TTGAAAAGGC CCTTAAGGCC 
CAGAACGACC TGATCTGGAA TGTCAAGGAC GAGCTAAAGA AAGCGTGCTC TACGAACGAC 
CTGAAAGAAC TGCTCATCTT CAACAAGCAG GAAGTGCCCT CCGGGGAGTC GGCGATCTTG 
GACCGCGTGG CCGACGGTAT GGTGTTTGGC GCCCTCCTTC CCTGCGAGGA ATGCTCGGGC 
CAGCTGGTCT TCAAGGGCGA CGCCTATTAC TGTACCGGGG ATGTGACTGC CTGGACCAAG 
TGTATGGTCA AGACACAGAC GCCCAACCGG AAGGAGTGGG TGACCCCAAA GGAATTCCGA 
GAAATCTCTT ACTTCAAGAA ACTGAAGATC AAAAAGCAGG ACCGTATATT CCCCCCAGAG 
AGCAGCACCC CGGTGGGGGC AGCAGCCCCA CCCTCTGCAG CTTCAGCGCC TGCCGCTGTG 
CACTCTGGCC CCCCAGACAA GCCATTATCC AACATGAAGA TCCTGACTCT CGGGAAACTC 
TCCCAGAACA AGGATGAAGT GAAGGCCACG ATTGAGAAAC TCGGGGGGAA ATTGACAGGG 
ACGGCCAACA AGGCCTCCCT GTGTATCAGC ACCAAAAAGG AGGTGGACAA GATGAATAAA 
AAGATGGAGG AAGTAAAAGA AGCCAACATC CGTGTCGTGT CTGAGGACTT CCTCCAAGAC 
ATCTCCGCCT CCACCAAGAG CCTTCAGGAG TTGCTCTCCA CCCACCTCTT GTCCCCCTGG 
GGAGCCGAGG TGAAGGTGGA GCCTGTTGAA GCAGTGGCCC CAAAGGGGAA GTCGGGGGCC 
GCGCCCTCCA AGAAGAGCAA GGGTCCCGTC AAGGAGGAAG GTACCAACAA ATCTGAAAAG 
AGGATGAAAT TAACTCTTAA AGGAGGAGCA GCTGTCGACC CTGATTCAGG TCTGGAACAC 
AATGCACACG TCCTCGAGAA AGGCGGGAAG GTCTTCAGCG CCACCCTCGG GCTTGTGGAC 
ATCGTCAAAG GGACCAACTC CTATTACAAG CTGCAGCTCC TGGAGGATGA CAAAGAGAGC 
AGGTACTGGA TATTCAGGTC CTGGGGCCGT GTGGGCACGG TGATTGGTAG TAACAAACTG 
GAGCAGATGC CATCCAAGGA GGATGCCATT GAGCATTTTA TGAAATTATA TGAAGAGAAA 
ACCGGAAACG CCTGGCACTC TAAAAACTTT ACGAAGCATC CCAAAAAGTT CTACCCTCTG 
GAGATTGACT ATGGCCAGGA TGAAGAGGCG GTGAAGAAGT TGACAGTAAA CCCTGGCACC 
AAGTCCAAGC TCCCCAAGCC AGTGCAGAAC CTCATTAAGA TGATCTTTGA TGTAGAGAGT 
ATGAAGAAAG CCATGGTGGA GTATGAGATT GACCTTCAGA AGATGCCCTT GGGGAAGCTG 
AGCAAAAGGC AGATCCAGGC TGCATACTCC ATCCTCAGTG AGGTCCAGCA GGCACTGTCC 
CAGGGCAGCA GTGACTCTCA CATCCTGGAT CTCTCCAACC GCTTCTACAC CCTGATCCCC 
CACGACTTCG GGATGAAGAA GCCCCCGCTG CTGAACAACG CAAACAGCGT GCAGGCCAAG 
GTGGAAATGC TAGACAACCT GCTGGATATT GAGGTGGCCT ACAGTCTACT CAGGGGTGGT 
TCTGATGACA GCAGCAAGGA CCCCATTGAT GTCAACTATG AGAAGCTCAA AACTGACATT 
AAGGTTGTGG ACAAAGATTC CGAAGAAGCC GAGATCATTA GGAAGTATGT GAAGAACACT 
CACGCGACCA CACACAACGC GTACGACTTA GAAGTCGTCG ACATCTTCAA GATAGAGCGC 
GAAGGGGAAA GCCAGCGTTA CAAGCCGTTT AAGCAGCTGC ATAACCGGAG GCTGCTGTGG 
CACGGGTCCA GGACCACCAA CTTCGCGGGC ATCCTGTCCC AGGGTCTCCG GATAGCCCCA 
CCTGAAGCAC CTGTGACGGG CTACATGTTT GGTAAGGGAA TCTATTTCGC CGACATGGTC 
TCCAAGAGTG CCAACTACTG CCACACGTCC CAGGGAGACC CAATAGGCTT GATCCTGTTG 
GGAGAAGTTG CCCTTGGAAA CATGTATGAA CTGAAACATG CTTCGCATAT CAGCAAGTTA 
CCCAAGGGCA AGCACAGTGT CAAAGGTTTA GGCAAAACTA CCCCTGACCC ATCGGCTAGT 
ATTACTGTGG ATGGTGTGGA GGTGCCTCTC GGGACAGGGA TTTCATCTGG TGTTAATGAC 
ACCTGTCTGC TGTATAACGA GTACATCGTC TACGACATTG CTCAGGTCCA CCTGAAGTAC 
CTGCTGAAGC TGAAGTTCAA CTTTAAGACA TCCCTGTGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assessment of calpain and caspase systems activities during ageing of two bovine muscles by degradation patterns of ?II spectrin and PARP-1.
Animal science journal = Nihon chikusan Gakkaiho87(3)462-6(2016 Mar)
Sacc? E,Pizzutti N,Corazzin M,Lippe G,Piasentier E

Lack of an association between two BER gene polymorphisms and breast cancer risk: a meta-analysis.
PloS one7(12)e50857(2012)
Wu B,Liu HL,Zhang S,Dong XR,Wu G

Poly(ADP-ribose) polymerase 1 interaction with apurinic/apyrimidinic sites.
Doklady. Biochemistry and biophysics43169-72(2010 Mar-Apr)
Khodyreva SN,Ilina ES,Kutuzov MM,Sukhanova MV,Lavrik OI

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

FGF2-mediated upregulation of urokinase-type plasminogen activator expression requires a MAP-kinase dependent activation of poly(ADP-ribose) polymerase.
Journal of cellular physiology202(1)125-34(2005 Jan)
Caldini R,Barletta E,Del Rosso M,Giovannelli L,Chevanne M

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PARP1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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