RFFL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFFL
Entrez Gene ID	117584
Full Name	ring finger and FYVE like domain containing E3 ubiquitin protein ligase
Synonyms	CARP-2,CARP2,FRING,RIFIFYLIN,RNF189,RNF34L
General protein information	Preferred Names ring finger and FYVE like domain containing E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase rififylin FYVE-RING finger protein SAKURA RING finger and FYVE-like domain-containing protein 1 RING finger protein 189 RING-type E3 ubiquitin transferase rififylin caspase 8 and 10 associated RING protein-2 caspase regulator CARP2 caspases-8 and -10-associated RING finger protein 2 ring finger and FYVE-like domain containing 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Disorder MIM:	609735

mRNA and Protein(s)

mRNA	Protein	Name
NM_001017368.2	NP_001017368.1	E3 ubiquitin-protein ligase rififylin
NM_001017368.1	NP_001017368.1	E3 ubiquitin-protein ligase rififylin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RFFL	XP_005624862.1
Rattus norvegicus (Norway rat)	Rffl	NP_001004068.1
Gallus gallus (chicken)	RFFL	NP_001025882.1
Homo sapiens (human)	RFFL	NP_001017368.1
Pan troglodytes (chimpanzee)	RFFL	XP_001174509.1
Bos taurus (cattle)	RFFL	XP_005220108.1
Xenopus tropicalis (tropical clawed frog)	rffl	NP_001017296.2
Mus musculus (house mouse)	Rffl	NP_001158042.1
Danio rerio (zebrafish)	zgc:77828	NP_998061.1

Gene Ontology
Bibliography

Related articles in PubMed

Positional cloning of quantitative trait nucleotides for blood pressure and cardiac QT-interval by targeted CRISPR/Cas9 editing of a novel long non-coding RNA.
Cheng X, Waghulde H, Mell B, Morgan EE, Pruett-Miller SM, Joe B
PLoS genetics13(8)e1006961(2017 Aug)

Different Raf protein kinases mediate different signaling pathways to stimulate E3 ligase RFFL gene expression in cell migration regulation.
Gan X, Wang C, Patel M, Kreutz B, Zhou M, Kozasa T, Wu D
The Journal of biological chemistry288(47)33978-84(2013 Nov)

PRR5L degradation promotes mTORC2-mediated PKC-? phosphorylation and cell migration downstream of G?12.
Gan X, Wang J, Wang C, Sommer E, Kozasa T, Srinivasula S, Alessi D, Offermanns S, Simon MI, Wu D
Nature cell biology14(7)686-96(2012 May)

Destabilization of CARP mRNAs by aloe-emodin contributes to caspase-8-mediated p53-independent apoptosis of human carcinoma cells.
Lin ML, Lu YC, Su HL, Lin HT, Lee CC, Kang SE, Lai TC, Chung JG, Chen SS
Journal of cellular biochemistry112(4)1176-91(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

it is interesting to note that a human lncRNA does exist within the 5'-UTR intronic region of the human RFFL gene . Given the rat data, our study may serve as a translational foundation for considering this human lncRNA as a candidate regulator for cardiovascular diseases.
Title: Positional cloning of quantitative trait nucleotides for blood pressure and cardiac QT-interval by targeted CRISPR/Cas9 editing of a novel long non-coding RNA.

EGF, which signals through CRAF, and an activated BRAF mutant also activate PKC and stimulate cell migration through up-regulating RFFL expression.
Title: Different Raf protein kinases mediate different signaling pathways to stimulate E3 ligase RFFL gene expression in cell migration regulation.

data indicate AE induces caspase-8-mediated activation of mitochondrial death pathways by decreasing the stability of CARP mRNAs in a p53-independent manner.
Title: Destabilization of CARP mRNAs by aloe-emodin contributes to caspase-8-mediated p53-independent apoptosis of human carcinoma cells.

Observational study of gene-disease association. (HuGE Navigator)
Title: An approach based on a genome-wide association study reveals candidate loci for narcolepsy.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association between genetic variants in VEGF, ERCC3 and occupational benzene haematotoxicity.

The following RFFL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFFL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04183	NM_001017368.2 Latest version!	Homo sapiens ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
View Old Accession Versions >>
OHu04183	NM_001017368.1	Homo sapiens ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFFL ( NM_001017368.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001017368.1 Homo sapiens ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04183
Clone ID Related Accession (Same CDS sequence)	NM_001017368.2 , NM_001017368.1
Accession Version	NM_001017368.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase rififylin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR933651.1, CK001659.1, BC028424.1 and AC004223.2. On Jun 2, 2019 this sequence version replaced NM_001017368.1. Transcript Variant: This variant (2) represents the shorter transcript but encodes the supported protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR933651.1, SRR1803614.47817.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGTCCCACCA 
CCCCAGGGAG CCAGGATGCA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCCCCA 
ACAGGCTTGG AACCAAGCTG CAAGTCCTGT GGGGCTCACT TTGCAAACAC GGCCAGGAAG 
CAGACCTGCT TGGACTGTAA GAAAAATTTT TGCATGACCT GTTCGAGCCA AGTAGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGG TTTCGAGCTA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACTTGAGG GACTATCTCA GCCTCCATGA CATCTCTACC 
GAAATGTGCC GGGAGAAAGA AGAGCTGGTG CTCTTGGTCC TTGGCCAGCA GCCTGTAATC 
TCCCAGGAGG ACAGGACTCG TGCCTCCACC TTGTCCCCAG ACTTTCCTGA GCAGCAGGCC 
TTCCTGACCC AGCCTCACTC CAGCATGGTT CCACCTACCT CACCCAACCT CCCCTCTTCA 
TCTGCACAAG CCACCTCTGT TCCCCCAGCC CAGGTTCAGG AGAATCAGCA GGCCAATGGC 
CATGTGTCTC AGGATCAAGA GGAACCCGTC TACCTGGAGA GCGTGGCCAG AGTACCTGCT 
GAGGATGAGA CCCAGTCTAT TGACTCAGAG GACAGCTTTG TCCCAGGCCG AAGGGCCTCT 
CTGTCTGACC TGACTGACCT GGAGGACATT GAAGGCCTGA CAGTGCGGCA GCTGAAAGAG 
ATCTTGGCTC GCAACTTTGT CAACTACAAG GGCTGCTGTG AGAAGTGGGA GCTGATGGAG 
AGAGTGACCC GGCTATACAA GGATCAGAAA GGACTCCAGC ACCTGGTCAG TGGTGCCGAA 
GACCAAAACG GGGGAGCAGT ACCATCAGGC TTGGAGGAGA ACCTGTGTAA GATCTGCATG 
GACTCACCCA TTGACTGTGT TCTTCTGGAG TGTGGCCACA TGGTAACCTG TACCAAGTGT 
GGCAAGCGCA TGAATGAATG TCCCATCTGC CGGCAGTATG TAATCCGAGC TGTGCATGTC 
TTCCGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017368.1
CDS	141..1232
Translation	MWATCCNWFCLDGQPEEVPPPQGARMQAYSNPGYSSFPSPTGLEPSCKSCGAHFANTARKQTCLDCKKNFCMTCSSQVGNGPRLCLLCQRFRATAFQREELMKMKVKDLRDYLSLHDISTEMCREKEELVLLVLGQQPVISQEDRTRASTLSPDFPEQQAFLTQPHSSMVPPTSPNLPSSSAQATSVPPAQVQENQQANGHVSQDQEEPVYLESVARVPAEDETQSIDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVSGAEDQNGGAVPSGLEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMNECPICRQYVIRAVHVFRS

Target ORF information:

RefSeq Version	NM_001017368.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017368.2

ORF Insert Sequence:

ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGTCCCACCA 
CCCCAGGGAG CCAGGATGCA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCCCCA 
ACAGGCTTGG AACCAAGCTG CAAGTCCTGT GGGGCTCACT TTGCAAACAC GGCCAGGAAG 
CAGACCTGCT TGGACTGTAA GAAAAATTTT TGCATGACCT GTTCGAGCCA AGTAGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGG TTTCGAGCTA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACTTGAGG GACTATCTCA GCCTCCATGA CATCTCTACC 
GAAATGTGCC GGGAGAAAGA AGAGCTGGTG CTCTTGGTCC TTGGCCAGCA GCCTGTAATC 
TCCCAGGAGG ACAGGACTCG TGCCTCCACC TTGTCCCCAG ACTTTCCTGA GCAGCAGGCC 
TTCCTGACCC AGCCTCACTC CAGCATGGTT CCACCTACCT CACCCAACCT CCCCTCTTCA 
TCTGCACAAG CCACCTCTGT TCCCCCAGCC CAGGTTCAGG AGAATCAGCA GGCCAATGGC 
CATGTGTCTC AGGATCAAGA GGAACCCGTC TACCTGGAGA GCGTGGCCAG AGTACCTGCT 
GAGGATGAGA CCCAGTCTAT TGACTCAGAG GACAGCTTTG TCCCAGGCCG AAGGGCCTCT 
CTGTCTGACC TGACTGACCT GGAGGACATT GAAGGCCTGA CAGTGCGGCA GCTGAAAGAG 
ATCTTGGCTC GCAACTTTGT CAACTACAAG GGCTGCTGTG AGAAGTGGGA GCTGATGGAG 
AGAGTGACCC GGCTATACAA GGATCAGAAA GGACTCCAGC ACCTGGTCAG TGGTGCCGAA 
GACCAAAACG GGGGAGCAGT ACCATCAGGC TTGGAGGAGA ACCTGTGTAA GATCTGCATG 
GACTCACCCA TTGACTGTGT TCTTCTGGAG TGTGGCCACA TGGTAACCTG TACCAAGTGT 
GGCAAGCGCA TGAATGAATG TCCCATCTGC CGGCAGTATG TAATCCGAGC TGTGCATGTC 
TTCCGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFFL ( NM_001017368.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001017368.1 Homo sapiens ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu04183
Clone ID Related Accession (Same CDS sequence)	NM_001017368.2 , NM_001017368.1
Accession Version	NM_001017368.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase rififylin
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR933651.1, CK001659.1 and BC028424.1. Transcript Variant: This variant (2) represents the shorter transcript but encodes the supported protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR933651.1, SRR1803611.198718.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGTCCCACCA 
CCCCAGGGAG CCAGGATGCA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCCCCA 
ACAGGCTTGG AACCAAGCTG CAAGTCCTGT GGGGCTCACT TTGCAAACAC GGCCAGGAAG 
CAGACCTGCT TGGACTGTAA GAAAAATTTT TGCATGACCT GTTCGAGCCA AGTAGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGG TTTCGAGCTA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACTTGAGG GACTATCTCA GCCTCCATGA CATCTCTACC 
GAAATGTGCC GGGAGAAAGA AGAGCTGGTG CTCTTGGTCC TTGGCCAGCA GCCTGTAATC 
TCCCAGGAGG ACAGGACTCG TGCCTCCACC TTGTCCCCAG ACTTTCCTGA GCAGCAGGCC 
TTCCTGACCC AGCCTCACTC CAGCATGGTT CCACCTACCT CACCCAACCT CCCCTCTTCA 
TCTGCACAAG CCACCTCTGT TCCCCCAGCC CAGGTTCAGG AGAATCAGCA GGCCAATGGC 
CATGTGTCTC AGGATCAAGA GGAACCCGTC TACCTGGAGA GCGTGGCCAG AGTACCTGCT 
GAGGATGAGA CCCAGTCTAT TGACTCAGAG GACAGCTTTG TCCCAGGCCG AAGGGCCTCT 
CTGTCTGACC TGACTGACCT GGAGGACATT GAAGGCCTGA CAGTGCGGCA GCTGAAAGAG 
ATCTTGGCTC GCAACTTTGT CAACTACAAG GGCTGCTGTG AGAAGTGGGA GCTGATGGAG 
AGAGTGACCC GGCTATACAA GGATCAGAAA GGACTCCAGC ACCTGGTCAG TGGTGCCGAA 
GACCAAAACG GGGGAGCAGT ACCATCAGGC TTGGAGGAGA ACCTGTGTAA GATCTGCATG 
GACTCACCCA TTGACTGTGT TCTTCTGGAG TGTGGCCACA TGGTAACCTG TACCAAGTGT 
GGCAAGCGCA TGAATGAATG TCCCATCTGC CGGCAGTATG TAATCCGAGC TGTGCATGTC 
TTCCGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017368.1
CDS	174..1265
Translation	MWATCCNWFCLDGQPEEVPPPQGARMQAYSNPGYSSFPSPTGLEPSCKSCGAHFANTARKQTCLDCKKNFCMTCSSQVGNGPRLCLLCQRFRATAFQREELMKMKVKDLRDYLSLHDISTEMCREKEELVLLVLGQQPVISQEDRTRASTLSPDFPEQQAFLTQPHSSMVPPTSPNLPSSSAQATSVPPAQVQENQQANGHVSQDQEEPVYLESVARVPAEDETQSIDSEDSFVPGRRASLSDLTDLEDIEGLTVRQLKEILARNFVNYKGCCEKWELMERVTRLYKDQKGLQHLVSGAEDQNGGAVPSGLEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMNECPICRQYVIRAVHVFRS

Target ORF information:

RefSeq Version	NM_001017368.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ring finger and FYVE like domain containing E3 ubiquitin protein ligase (RFFL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017368.1

ORF Insert Sequence:

ATGTGGGCAA CCTGCTGCAA CTGGTTCTGC CTGGATGGAC AGCCTGAGGA GGTCCCACCA 
CCCCAGGGAG CCAGGATGCA GGCCTATTCC AACCCTGGGT ACAGCTCCTT CCCTTCCCCA 
ACAGGCTTGG AACCAAGCTG CAAGTCCTGT GGGGCTCACT TTGCAAACAC GGCCAGGAAG 
CAGACCTGCT TGGACTGTAA GAAAAATTTT TGCATGACCT GTTCGAGCCA AGTAGGGAAT 
GGGCCCCGCC TCTGCCTTCT CTGCCAACGG TTTCGAGCTA CAGCCTTTCA GCGAGAGGAG 
CTCATGAAGA TGAAGGTGAA GGACTTGAGG GACTATCTCA GCCTCCATGA CATCTCTACC 
GAAATGTGCC GGGAGAAAGA AGAGCTGGTG CTCTTGGTCC TTGGCCAGCA GCCTGTAATC 
TCCCAGGAGG ACAGGACTCG TGCCTCCACC TTGTCCCCAG ACTTTCCTGA GCAGCAGGCC 
TTCCTGACCC AGCCTCACTC CAGCATGGTT CCACCTACCT CACCCAACCT CCCCTCTTCA 
TCTGCACAAG CCACCTCTGT TCCCCCAGCC CAGGTTCAGG AGAATCAGCA GGCCAATGGC 
CATGTGTCTC AGGATCAAGA GGAACCCGTC TACCTGGAGA GCGTGGCCAG AGTACCTGCT 
GAGGATGAGA CCCAGTCTAT TGACTCAGAG GACAGCTTTG TCCCAGGCCG AAGGGCCTCT 
CTGTCTGACC TGACTGACCT GGAGGACATT GAAGGCCTGA CAGTGCGGCA GCTGAAAGAG 
ATCTTGGCTC GCAACTTTGT CAACTACAAG GGCTGCTGTG AGAAGTGGGA GCTGATGGAG 
AGAGTGACCC GGCTATACAA GGATCAGAAA GGACTCCAGC ACCTGGTCAG TGGTGCCGAA 
GACCAAAACG GGGGAGCAGT ACCATCAGGC TTGGAGGAGA ACCTGTGTAA GATCTGCATG 
GACTCACCCA TTGACTGTGT TCTTCTGGAG TGTGGCCACA TGGTAACCTG TACCAAGTGT 
GGCAAGCGCA TGAATGAATG TCCCATCTGC CGGCAGTATG TAATCCGAGC TGTGCATGTC 
TTCCGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Positional cloning of quantitative trait nucleotides for blood pressure and cardiac QT-interval by targeted CRISPR/Cas9 editing of a novel long non-coding RNA.
PLoS genetics13(8)e1006961(2017 Aug)
Cheng X,Waghulde H,Mell B,Morgan EE,Pruett-Miller SM,Joe B

Different Raf protein kinases mediate different signaling pathways to stimulate E3 ligase RFFL gene expression in cell migration regulation.
The Journal of biological chemistry288(47)33978-84(2013 Nov)
Gan X,Wang C,Patel M,Kreutz B,Zhou M,Kozasa T,Wu D

PRR5L degradation promotes mTORC2-mediated PKC-? phosphorylation and cell migration downstream of G?12.
Nature cell biology14(7)686-96(2012 May)
Gan X,Wang J,Wang C,Sommer E,Kozasa T,Srinivasula S,Alessi D,Offermanns S,Simon MI,Wu D

Destabilization of CARP mRNAs by aloe-emodin contributes to caspase-8-mediated p53-independent apoptosis of human carcinoma cells.
Journal of cellular biochemistry112(4)1176-91(2011 Apr)
Lin ML,Lu YC,Su HL,Lin HT,Lee CC,Kang SE,Lai TC,Chung JG,Chen SS

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RFFL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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