rffl cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following rffl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rffl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa22722 NM_001017296.3
Latest version!
Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa28174 XM_012957057.2
Latest version!
Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $174.30
$249.00
OXa42565 XM_018091282.1
Latest version!
Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $174.30
$249.00
OXa51393 XM_012957057.3
Latest version!
Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa22722
    Clone ID Related Accession (Same CDS sequence) NM_001017296.3
    Accession Version NM_001017296.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase rififylin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC090590.1. On Nov 18, 2005 this sequence version replaced NM_001017296.2. ##Evidence-Data-START## Transcript exon combination :: BC090590.1, CR855622.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00028290, SAMD00028291 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTGGGCTG CCTGCTGTAA CTGGCTGTGT TTTGATACAG AACCACATGA AATTTCACAG 
    AGTGACAGAA CACAAGCTTT TTCCAATTCA AACTTCAGGA CCTCTCCTCC TGTTTCAGAA
    ATCTCCTGCA AAGCTTGTGG TATCCGTTTT GCGGCATGTG CTAGAAAGAA CCCCTGCATG
    GATTGCAAGA ATTACTACTG TGCAGCATGT CTAATCCAGA CAAACAATGG CCATTCCCTT
    TGTCAAGTAT GCAGAAGCAT CCGCTCCACA GCCTTTGACC GAGATGAGTT GATGAAGCTA
    AGAGTAAAAG ACTTGCGGGA TTACCTAACT TTAAAAGACA TTCCCATAGA CCTTTGTCGA
    GAAAAGGAAG ATCTTGTACA GCTTATTTTG TTTCATAAAC CCCCAGTTTT TAGTGCCGAT
    GGAACCAATG TGCCTACTGC TAGTTCTAAC CAAAGCACCA CAAATCAAAG AACAGATTCC
    AGCTACACAC CCGGGCACCC CCCTTACAAT ACTTCAGCAG CAAACAGAAC TGGTTCATCC
    AATGCCCAGG GCACAGCTGG AGAACATTTG GTGGATTCTG AATCATCTGA CCCAGAGGAG
    AATCTGGCAC CTGGGAGGAG GGCATCCCTC TCTGATCTCA CCTGTGTGGA TGACATTGAA
    GCCCTTTCCG TGCGTCAGCT AAAAGAGATC TTGGCCAGAA ATTTTGTTGA CTATAAAGGC
    TGCTGTGAGA AGTGGGAACT CATGGAACGT GTTACCCGGT TATACAATGA ACAAAAGGGT
    CTTAAGGAGA TGGCTGCTGC TGAAGCAGAA GGCATTAATG GTACAACAAG CGAAGAGAAT
    ATCTGCAAGG TCTGCATGGA CTCCCCTATC GACTGTGTTC TCTTAGAGTG TGGCCATATG
    GTGACTTGCA CCAAATGTGG CAAGCGCATG AGTGAATGCC CTATCTGTCG ACAGTATGTA
    GTGAGAGCAG TGCATGTATT CCGTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001017296.2
    CDS103..1092
    Translation

    Target ORF information:

    RefSeq Version NM_001017296.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001017296.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTGGGCTG CCTGCTGTAA CTGGCTGTGT TTTGATACAG AACCACATGA AATTTCACAG 
    AGTGACAGAA CACAAGCTTT TTCCAATTCA AACTTCAGGA CCTCTCCTCC TGTTTCAGAA
    ATCTCCTGCA AAGCTTGTGG TATCCGTTTT GCGGCATGTG CTAGAAAGAA CCCCTGCATG
    GATTGCAAGA ATTACTACTG TGCAGCATGT CTAATCCAGA CAAACAATGG CCATTCCCTT
    TGTCAAGTAT GCAGAAGCAT CCGCTCCACA GCCTTTGACC GAGATGAGTT GATGAAGCTA
    AGAGTAAAAG ACTTGCGGGA TTACCTAACT TTAAAAGACA TTCCCATAGA CCTTTGTCGA
    GAAAAGGAAG ATCTTGTACA GCTTATTTTG TTTCATAAAC CCCCAGTTTT TAGTGCCGAT
    GGAACCAATG TGCCTACTGC TAGTTCTAAC CAAAGCACCA CAAATCAAAG AACAGATTCC
    AGCTACACAC CCGGGCACCC CCCTTACAAT ACTTCAGCAG CAAACAGAAC TGGTTCATCC
    AATGCCCAGG GCACAGCTGG AGAACATTTG GTGGATTCTG AATCATCTGA CCCAGAGGAG
    AATCTGGCAC CTGGGAGGAG GGCATCCCTC TCTGATCTCA CCTGTGTGGA TGACATTGAA
    GCCCTTTCCG TGCGTCAGCT AAAAGAGATC TTGGCCAGAA ATTTTGTTGA CTATAAAGGC
    TGCTGTGAGA AGTGGGAACT CATGGAACGT GTTACCCGGT TATACAATGA ACAAAAGGGT
    CTTAAGGAGA TGGCTGCTGC TGAAGCAGAA GGCATTAATG GTACAACAAG CGAAGAGAAT
    ATCTGCAAGG TCTGCATGGA CTCCCCTATC GACTGTGTTC TCTTAGAGTG TGGCCATATG
    GTGACTTGCA CCAAATGTGG CAAGCGCATG AGTGAATGCC CTATCTGTCG ACAGTATGTA
    GTGAGAGCAG TGCATGTATT CCGTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa28174
    Clone ID Related Accession (Same CDS sequence) XM_012957057.2
    Accession Version XM_012957057.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase rififylin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012957057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTGCA AGAATTACTA CTGTGCAGCA TGTCTAATCC AGACAAACAA TGGCCATTCC 
    CTTTGTCAAG TATGCAGAAG CATCCGCTCC ACAGCCTTTG ACCGAGATGA GTTGATGAAG
    CTAAGAGTAA AAGACTTGCG GGATTACCTA ACTTTAAAAG ACATTCCCAT AGACCTTTGT
    CGAGAAAAGG AAGATCTTGT ACAGCTTATT TTGTTTCATA AACCCCCAGT TTTTAGTGCC
    GATGGAACCA ATGTGCCTAC TGCTAGTTCT AACCAAAGCA CCACAAATCA AAGAACAGAT
    TCCAGCTACA CACCCGGGCA CCCCCCTTAC AATACTTCAG CAGCAAACAG AACTGGTTCA
    TCCAATGCCC AGGGCACAGC TGGAGAACAT TTGGTGGATT CTGAATCATC TGACCCAGAG
    GAGAATCTGG CACCTGGGAG GAGGGCATCC CTCTCTGATC TCACCTGTGT GGATGACATT
    GAAGCCCTTT CCGTGCGTCA GCTAAAAGAG ATCTTGGCCA GAAATTTTGT TGACTATAAA
    GGCTGCTGTG AGAAGTGGGA ACTCATGGAA CGTGTTACCC GGTTATACAA TGAACAAAAG
    GGTCTTCAGG AGATGGCTGC TGCTGAAGCA GAAGGCATTA ATGGTACAAC AAGCGAAGAG
    AATATCTGCA AGGTCTGCAT GGACTCCCCT ATCGACTGTG TTCTCTTAGA GTGTGGCCAT
    ATGGTGACTT GCACCAAATG TGGCAAGCGC ATGAGTGAAT GCCCTATCTG TCGACAGTAT
    GTAGTGAGAG CAGTGCATGT ATTCCGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812511.1
    CDS301..1113
    Translation

    Target ORF information:

    RefSeq Version XM_012957057.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTGCA AGAATTACTA CTGTGCAGCA TGTCTAATCC AGACAAACAA TGGCCATTCC 
    CTTTGTCAAG TATGCAGAAG CATCCGCTCC ACAGCCTTTG ACCGAGATGA GTTGATGAAG
    CTAAGAGTAA AAGACTTGCG GGATTACCTA ACTTTAAAAG ACATTCCCAT AGACCTTTGT
    CGAGAAAAGG AAGATCTTGT ACAGCTTATT TTGTTTCATA AACCCCCAGT TTTTAGTGCC
    GATGGAACCA ATGTGCCTAC TGCTAGTTCT AACCAAAGCA CCACAAATCA AAGAACAGAT
    TCCAGCTACA CACCCGGGCA CCCCCCTTAC AATACTTCAG CAGCAAACAG AACTGGTTCA
    TCCAATGCCC AGGGCACAGC TGGAGAACAT TTGGTGGATT CTGAATCATC TGACCCAGAG
    GAGAATCTGG CACCTGGGAG GAGGGCATCC CTCTCTGATC TCACCTGTGT GGATGACATT
    GAAGCCCTTT CCGTGCGTCA GCTAAAAGAG ATCTTGGCCA GAAATTTTGT TGACTATAAA
    GGCTGCTGTG AGAAGTGGGA ACTCATGGAA CGTGTTACCC GGTTATACAA TGAACAAAAG
    GGTCTTCAGG AGATGGCTGC TGCTGAAGCA GAAGGCATTA ATGGTACAAC AAGCGAAGAG
    AATATCTGCA AGGTCTGCAT GGACTCCCCT ATCGACTGTG TTCTCTTAGA GTGTGGCCAT
    ATGGTGACTT GCACCAAATG TGGCAAGCGC ATGAGTGAAT GCCCTATCTG TCGACAGTAT
    GTAGTGAGAG CAGTGCATGT ATTCCGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42565
    Clone ID Related Accession (Same CDS sequence) XM_018091282.1
    Accession Version XM_018091282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTGGGCTG CCTGCTGTAA CTGGCTGTGT TTTGATACAG AACCACATGA AATTTCACAG 
    AGTGACAGAA CACAAGCTTT TTCCAATTCA AACTTCAGGA CCTCTCCTCC TGTTTCAGAA
    ATCTCCTGCA AAGCTTGTGG TATCCGTTTT GCGGCATGTG CTAGAAAGAA CCCCTGCATG
    GATTGCAAGA ATTACTACTG TGCAGCATGT CTAATCCAGA CAAACAATGG CCATTCCCTT
    TGTCAAGTAT GCAGAAGCAT CCGCTCCACA GCCTTTGACC GAGATGAGTT GATGAAGCTA
    AGAGTAAAAG ACTTGCGGGA TTACCTAACT TTAAAAGACA TTCCCATAGA CCTTTGTCGA
    GAAAAGGAAG ATCTTGTACA GCTTATTTTG TTTCATAAAC CCCCAGTTTT TAGTGCCGAT
    GGAACCAATG TGCCTACTGC TAGTTCTAAC CAAAGCACCA CAAATCAAAG AACAGATTCC
    AGCTACACAC CCGGGCACCC CCCTTACAAT ACTTCAGCAG CAAACAGAAC TGGTTCATCC
    AATGCCCAGG GCACAGCTGG AGAACATTTG GTGGATTCTG AATCATCTGA CCCAGAGGAG
    AATCTGGCAC CTGGGAGGAG GGCATCCCTC TCTGATCTCA CCTGTGTGGA TGACATTGAA
    GCCCTTTCCG TGCGTCAGCT AAAAGAGATC TTGGCCAGAA ATTTTGTTGA CTATAAAGGC
    TGCTGTGAGA AGTGGGAACT CATGGAACGT GTTACCCGGT TATACAATGA ACAAAAGGGT
    CTTCAGGAGA TGGCTGCTGC TGAAGCAGAA GGCATTAATG GTACAACAAG CGAAGAGAAT
    ATCTGCAAGG TCTGCATGGA CTCCCCTATC GACTGTGTTC TCTTAGAGTG TGGCCATATG
    GTGACTTGCA CCAAATGTGG CAAGCGCATG AGTGAATGCC CTATCTGTCG ACAGTATGTA
    GTGAGAGCAG TGCATGTATT CCGTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017946771.1
    CDS228..1217
    Translation

    Target ORF information:

    RefSeq Version XM_018091282.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018091282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTGGGCTG CCTGCTGTAA CTGGCTGTGT TTTGATACAG AACCACATGA AATTTCACAG 
    AGTGACAGAA CACAAGCTTT TTCCAATTCA AACTTCAGGA CCTCTCCTCC TGTTTCAGAA
    ATCTCCTGCA AAGCTTGTGG TATCCGTTTT GCGGCATGTG CTAGAAAGAA CCCCTGCATG
    GATTGCAAGA ATTACTACTG TGCAGCATGT CTAATCCAGA CAAACAATGG CCATTCCCTT
    TGTCAAGTAT GCAGAAGCAT CCGCTCCACA GCCTTTGACC GAGATGAGTT GATGAAGCTA
    AGAGTAAAAG ACTTGCGGGA TTACCTAACT TTAAAAGACA TTCCCATAGA CCTTTGTCGA
    GAAAAGGAAG ATCTTGTACA GCTTATTTTG TTTCATAAAC CCCCAGTTTT TAGTGCCGAT
    GGAACCAATG TGCCTACTGC TAGTTCTAAC CAAAGCACCA CAAATCAAAG AACAGATTCC
    AGCTACACAC CCGGGCACCC CCCTTACAAT ACTTCAGCAG CAAACAGAAC TGGTTCATCC
    AATGCCCAGG GCACAGCTGG AGAACATTTG GTGGATTCTG AATCATCTGA CCCAGAGGAG
    AATCTGGCAC CTGGGAGGAG GGCATCCCTC TCTGATCTCA CCTGTGTGGA TGACATTGAA
    GCCCTTTCCG TGCGTCAGCT AAAAGAGATC TTGGCCAGAA ATTTTGTTGA CTATAAAGGC
    TGCTGTGAGA AGTGGGAACT CATGGAACGT GTTACCCGGT TATACAATGA ACAAAAGGGT
    CTTCAGGAGA TGGCTGCTGC TGAAGCAGAA GGCATTAATG GTACAACAAG CGAAGAGAAT
    ATCTGCAAGG TCTGCATGGA CTCCCCTATC GACTGTGTTC TCTTAGAGTG TGGCCATATG
    GTGACTTGCA CCAAATGTGG CAAGCGCATG AGTGAATGCC CTATCTGTCG ACAGTATGTA
    GTGAGAGCAG TGCATGTATT CCGTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa51393
    Clone ID Related Accession (Same CDS sequence) XM_012957057.3
    Accession Version XM_012957057.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase rififylin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012957057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTGCA AGAATTACTA CTGTGCAGCA TGTCTAATCC AGACAAACAA TGGCCATTCC 
    CTTTGTCAAG TATGCAGAAG CATCCGCTCC ACAGCCTTTG ACCGAGATGA GTTGATGAAG
    CTAAGAGTAA AAGACTTGCG GGATTACCTA ACTTTAAAAG ACATTCCCAT AGACCTTTGT
    CGAGAAAAGG AAGATCTTGT ACAGCTTATT TTGTTTCATA AACCCCCAGT TTTTAGTGCC
    GATGGAACCA ATGTGCCTAC TGCTAGTTCT AACCAAAGCA CCACAAATCA AAGAACAGAT
    TCCAGCTACA CACCCGGGCA CCCCCCTTAC AATACTTCAG CAGCAAACAG AACTGGTTCA
    TCCAATGCCC AGGGCACAGC TGGAGAACAT TTGGTGGATT CTGAATCATC TGACCCAGAG
    GAGAATCTGG CACCTGGGAG GAGGGCATCC CTCTCTGATC TCACCTGTGT GGATGACATT
    GAAGCCCTTT CCGTGCGTCA GCTAAAAGAG ATCTTGGCCA GAAATTTTGT TGACTATAAA
    GGCTGCTGTG AGAAGTGGGA ACTCATGGAA CGTGTTACCC GGTTATACAA TGAACAAAAG
    GGTCTTAAGG AGATGGCTGC TGCTGAAGCA GAAGGCATTA ATGGTACAAC AAGCGAAGAG
    AATATCTGCA AGGTCTGCAT GGACTCCCCT ATCGACTGTG TTCTCTTAGA GTGTGGCCAT
    ATGGTGACTT GCACCAAGTG TGGCAAGCGC ATGAGTGAAT GCCCTATCTG TCGACAGTAT
    GTAGTGAGAG CAGTGCATGT ATTCCGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012812511.2
    CDS300..1112
    Translation

    Target ORF information:

    RefSeq Version XM_012957057.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012957057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGATTGCA AGAATTACTA CTGTGCAGCA TGTCTAATCC AGACAAACAA TGGCCATTCC 
    CTTTGTCAAG TATGCAGAAG CATCCGCTCC ACAGCCTTTG ACCGAGATGA GTTGATGAAG
    CTAAGAGTAA AAGACTTGCG GGATTACCTA ACTTTAAAAG ACATTCCCAT AGACCTTTGT
    CGAGAAAAGG AAGATCTTGT ACAGCTTATT TTGTTTCATA AACCCCCAGT TTTTAGTGCC
    GATGGAACCA ATGTGCCTAC TGCTAGTTCT AACCAAAGCA CCACAAATCA AAGAACAGAT
    TCCAGCTACA CACCCGGGCA CCCCCCTTAC AATACTTCAG CAGCAAACAG AACTGGTTCA
    TCCAATGCCC AGGGCACAGC TGGAGAACAT TTGGTGGATT CTGAATCATC TGACCCAGAG
    GAGAATCTGG CACCTGGGAG GAGGGCATCC CTCTCTGATC TCACCTGTGT GGATGACATT
    GAAGCCCTTT CCGTGCGTCA GCTAAAAGAG ATCTTGGCCA GAAATTTTGT TGACTATAAA
    GGCTGCTGTG AGAAGTGGGA ACTCATGGAA CGTGTTACCC GGTTATACAA TGAACAAAAG
    GGTCTTAAGG AGATGGCTGC TGCTGAAGCA GAAGGCATTA ATGGTACAAC AAGCGAAGAG
    AATATCTGCA AGGTCTGCAT GGACTCCCCT ATCGACTGTG TTCTCTTAGA GTGTGGCCAT
    ATGGTGACTT GCACCAAGTG TGGCAAGCGC ATGAGTGAAT GCCCTATCTG TCGACAGTAT
    GTAGTGAGAG CAGTGCATGT ATTCCGTTCC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.