rffl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	rffl
Entrez Gene ID	405832
Full Name	ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
Synonyms	zgc:77828
General protein information	Preferred Names ring finger and FYVE-like domain containing E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase rififylin rififylin-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009291450.2	XP_009289725.1	E3 ubiquitin-protein ligase rififylin isoform X1
XM_005157394.3	XP_005157451.1	E3 ubiquitin-protein ligase rififylin isoform X2
XM_005157394.4	XP_005157451.1	E3 ubiquitin-protein ligase rififylin isoform X2
NM_212896.1	NP_998061.1	E3 ubiquitin-protein ligase rififylin
XM_009291450.3	XP_009289725.1	E3 ubiquitin-protein ligase rififylin isoform X1
XM_021481368.1	XP_021337043.1	E3 ubiquitin-protein ligase rififylin isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RFFL	XP_005624862.1
Rattus norvegicus (Norway rat)	Rffl	NP_001004068.1
Gallus gallus (chicken)	RFFL	NP_001025882.1
Homo sapiens (human)	RFFL	NP_001017368.1
Pan troglodytes (chimpanzee)	RFFL	XP_001174509.1
Bos taurus (cattle)	RFFL	XP_005220108.1
Xenopus tropicalis (tropical clawed frog)	rffl	NP_001017296.2
Mus musculus (house mouse)	Rffl	NP_001158042.1
Danio rerio (zebrafish)	zgc:77828	NP_998061.1

Gene Ontology
Bibliography

Related articles in PubMed

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Smith LC, Clark JC, Bisesi JH Jr, Ferguson PL, Sabo-Attwood T
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rffl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rffl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31651	NM_212896.1 Latest version!	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa20173	XM_005157394.4 Latest version!	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa40821	XM_009291450.3 Latest version!	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa148936	XM_021481368.1 Latest version!	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
ODa20173	XM_005157394.3	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa40821	XM_009291450.2	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rffl ( NM_212896.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998061.1 Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), mRNA.

Price & Availability↑

CloneID	ODa31651
Clone ID Related Accession (Same CDS sequence)	NM_212896.1
Accession Version	NM_212896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase rififylin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067339.1. ##Evidence-Data-START## Transcript exon combination :: BC067339.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGGGCCA GCTGTTGCGG CTGGCTCTGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACAAAC TCAGGCTTCA CCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAGACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGTTTTCA GACTCAGAGG AAGTTTTGGC TCCTGGCCGA 
CGGGCGTCTC TCTCAGACCT AGGATCAGTG GAGGATATTG AGGATTTAAG TGTCCGGCAG 
TTGAAGGAGA TTCTAGCCCG AAACTTTGTC GACTACAAGG GCTGCTGTGA GAAATGGGAG 
TTGATGGAGA GGGTCACCCG CCTGTACAAC GACCAGAAGG ACCTGCAAAA CATGGTGTCT 
AATGCTACAG AAGGCACAGA CACTGGCTCT GGGACGGCCG TGGAGGAGAA CCTCTGTAAG 
ATCTGTATGG ACTCACCCAT CGACTGTGTG CTTCTGGAGT GCGGACACAT GGTCACGTGC 
ACAAAGTGTG GCAAACGCAT GAGCGAATGT CCCATCTGCC GACAGTACGT GGTGCGGGCA 
GTGCACGTGT TCAGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998061.1
CDS	273..1313
Translation	MWASCCGWLCLDDSSMEDTRGSQQRQAFTNSGFTSYPSPTAPEHTCKSCGGRFDSLTRKHVCMDCKKSYCSQCSTQQELQPWLCRTCQRFRGILLERAELMKLKVKDLRDYLHLHGVPTQMCREKEELVELVLGQQTPTSDTHIPAPETTRLDQQETLDTPVITVSPSGNNNTTELGPQTQAMDNGQSLAEVTAESEMDTELQMEQEQFSDSEEVLAPGRRASLSDLGSVEDIEDLSVRQLKEILARNFVDYKGCCEKWELMERVTRLYNDQKDLQNMVSNATEGTDTGSGTAVEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	NM_212896.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212896.1

ORF Insert Sequence:

ATGTGGGCCA GCTGTTGCGG CTGGCTCTGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACAAAC TCAGGCTTCA CCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAGACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGTTTTCA GACTCAGAGG AAGTTTTGGC TCCTGGCCGA 
CGGGCGTCTC TCTCAGACCT AGGATCAGTG GAGGATATTG AGGATTTAAG TGTCCGGCAG 
TTGAAGGAGA TTCTAGCCCG AAACTTTGTC GACTACAAGG GCTGCTGTGA GAAATGGGAG 
TTGATGGAGA GGGTCACCCG CCTGTACAAC GACCAGAAGG ACCTGCAAAA CATGGTGTCT 
AATGCTACAG AAGGCACAGA CACTGGCTCT GGGACGGCCG TGGAGGAGAA CCTCTGTAAG 
ATCTGTATGG ACTCACCCAT CGACTGTGTG CTTCTGGAGT GCGGACACAT GGTCACGTGC 
ACAAAGTGTG GCAAACGCAT GAGCGAATGT CCCATCTGCC GACAGTACGT GGTGCGGGCA 
GTGCACGTGT TCAGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rffl ( XM_005157394.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157451.1 Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20173
Clone ID Related Accession (Same CDS sequence)	XM_005157394.3 , XM_005157394.4
Accession Version	XM_005157394.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase rififylin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GTTTTCAGAC TCAGAGGAAG TTTTGGCTCC TGGCCGACGG 
GCGTCTCTCT CAGACCTAGG ATCAGTGGAG GATATTGAGG ATTTAAGTGT CCGGCAGTTG 
AAGGAGATTC TAGCCCGAAA CTTTGTCGAC TACAAGGGCT GCTGTGAGAA ATGGGAGTTG 
ATGGAGAGGG TCACCCGCCT GTACAACGAC CAGAAGGACC TGCAAAACAT GGTGTCTAAT 
GCTACAGAAG GCACAGACAC TGGCTCTGGG ACGGCCGTGG AGGAGAACCT CTGTAAGATC 
TGTATGGACT CACCCATCGA CTGTGTGCTT CTGGAGTGCG GACACATGGT CACGTGCACA 
AAGTGTGGCA AACGCATGAG CGAATGTCCC ATCTGCCGAC AGTACGTGGT GCGGGCAGTG 
CACGTGTTCA GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157451.1
CDS	310..1287
Translation	MWASCCGWLCLDDSSMEDTRGSQQRQAFTNSGFSSYPSPTAPEHTCKSCGGRFDSLTRKHVCMDCKKSYCSQCSTQQELQPWLCRTCQRFRGILLERAELMKLKVKDLRDYLHLHGVPTQMCREKEELVELVLGQQTPTSDTHIPAPETTRLDQQETLDTPVITVSPSGNNNTTELGPQTQAMDNGQFSDSEEVLAPGRRASLSDLGSVEDIEDLSVRQLKEILARNFVDYKGCCEKWELMERVTRLYNDQKDLQNMVSNATEGTDTGSGTAVEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	XM_005157394.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157394.3

ORF Insert Sequence:

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GTTTTCAGAC TCAGAGGAAG TTTTGGCTCC TGGCCGACGG 
GCGTCTCTCT CAGACCTAGG ATCAGTGGAG GATATTGAGG ATTTAAGTGT CCGGCAGTTG 
AAGGAGATTC TAGCCCGAAA CTTTGTCGAC TACAAGGGCT GCTGTGAGAA ATGGGAGTTG 
ATGGAGAGGG TCACCCGCCT GTACAACGAC CAGAAGGACC TGCAAAACAT GGTGTCTAAT 
GCTACAGAAG GCACAGACAC TGGCTCTGGG ACGGCCGTGG AGGAGAACCT CTGTAAGATC 
TGTATGGACT CACCCATCGA CTGTGTGCTT CTGGAGTGCG GACACATGGT CACGTGCACA 
AAGTGTGGCA AACGCATGAG CGAATGTCCC ATCTGCCGAC AGTACGTGGT GCGGGCAGTG 
CACGTGTTCA GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rffl ( XM_005157394.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157451.1 Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20173
Clone ID Related Accession (Same CDS sequence)	XM_005157394.3 , XM_005157394.4
Accession Version	XM_005157394.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase rififylin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157394.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GTTTTCAGAC TCAGAGGAAG TTTTGGCTCC TGGCCGACGG 
GCGTCTCTCT CAGACCTAGG ATCAGTGGAG GATATTGAGG ATTTAAGTGT CCGGCAGTTG 
AAGGAGATTC TAGCCCGAAA CTTTGTCGAC TACAAGGGCT GCTGTGAGAA ATGGGAGTTG 
ATGGAGAGGG TCACCCGCCT GTACAACGAC CAGAAGGACC TGCAAAACAT GGTGTCTAAT 
GCTACAGAAG GCACAGACAC TGGCTCTGGG ACGGCCGTGG AGGAGAACCT CTGTAAGATC 
TGTATGGACT CACCCATCGA CTGTGTGCTT CTGGAGTGCG GACACATGGT CACGTGCACA 
AAGTGTGGCA AACGCATGAG CGAATGTCCC ATCTGCCGAC AGTACGTGGT GCGGGCAGTG 
CACGTGTTCA GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157451.1
CDS	315..1292
Translation	MWASCCGWLCLDDSSMEDTRGSQQRQAFTNSGFSSYPSPTAPEHTCKSCGGRFDSLTRKHVCMDCKKSYCSQCSTQQELQPWLCRTCQRFRGILLERAELMKLKVKDLRDYLHLHGVPTQMCREKEELVELVLGQQTPTSDTHIPAPETTRLDQQETLDTPVITVSPSGNNNTTELGPQTQAMDNGQFSDSEEVLAPGRRASLSDLGSVEDIEDLSVRQLKEILARNFVDYKGCCEKWELMERVTRLYNDQKDLQNMVSNATEGTDTGSGTAVEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	XM_005157394.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157394.4

ORF Insert Sequence:

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GTTTTCAGAC TCAGAGGAAG TTTTGGCTCC TGGCCGACGG 
GCGTCTCTCT CAGACCTAGG ATCAGTGGAG GATATTGAGG ATTTAAGTGT CCGGCAGTTG 
AAGGAGATTC TAGCCCGAAA CTTTGTCGAC TACAAGGGCT GCTGTGAGAA ATGGGAGTTG 
ATGGAGAGGG TCACCCGCCT GTACAACGAC CAGAAGGACC TGCAAAACAT GGTGTCTAAT 
GCTACAGAAG GCACAGACAC TGGCTCTGGG ACGGCCGTGG AGGAGAACCT CTGTAAGATC 
TGTATGGACT CACCCATCGA CTGTGTGCTT CTGGAGTGCG GACACATGGT CACGTGCACA 
AAGTGTGGCA AACGCATGAG CGAATGTCCC ATCTGCCGAC AGTACGTGGT GCGGGCAGTG 
CACGTGTTCA GATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rffl ( XM_009291450.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289725.1 Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40821
Clone ID Related Accession (Same CDS sequence)	XM_009291450.2 , XM_009291450.3
Accession Version	XM_009291450.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase rififylin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGTTTTCA GACTCAGAGG AAGTTTTGGC TCCTGGCCGA 
CGGGCGTCTC TCTCAGACCT AGGATCAGTG GAGGATATTG AGGATTTAAG TGTCCGGCAG 
TTGAAGGAGA TTCTAGCCCG AAACTTTGTC GACTACAAGG GCTGCTGTGA GAAATGGGAG 
TTGATGGAGA GGGTCACCCG CCTGTACAAC GACCAGAAGG ACCTGCAAAA CATGGTGTCT 
AATGCTACAG AAGGCACAGA CACTGGCTCT GGGACGGCCG TGGAGGAGAA CCTCTGTAAG 
ATCTGTATGG ACTCACCCAT CGACTGTGTG CTTCTGGAGT GCGGACACAT GGTCACGTGC 
ACAAAGTGTG GCAAACGCAT GAGCGAATGT CCCATCTGCC GACAGTACGT GGTGCGGGCA 
GTGCACGTGT TCAGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289725.1
CDS	308..1348
Translation	MWASCCGWLCLDDSSMEDTRGSQQRQAFTNSGFSSYPSPTAPEHTCKSCGGRFDSLTRKHVCMDCKKSYCSQCSTQQELQPWLCRTCQRFRGILLERAELMKLKVKDLRDYLHLHGVPTQMCREKEELVELVLGQQTPTSDTHIPAPETTRLDQQETLDTPVITVSPSGNNNTTELGPQTQAMDNGQSLAEVTAESEMDTELQMEQEQFSDSEEVLAPGRRASLSDLGSVEDIEDLSVRQLKEILARNFVDYKGCCEKWELMERVTRLYNDQKDLQNMVSNATEGTDTGSGTAVEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	XM_009291450.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291450.2

ORF Insert Sequence:

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGTTTTCA GACTCAGAGG AAGTTTTGGC TCCTGGCCGA 
CGGGCGTCTC TCTCAGACCT AGGATCAGTG GAGGATATTG AGGATTTAAG TGTCCGGCAG 
TTGAAGGAGA TTCTAGCCCG AAACTTTGTC GACTACAAGG GCTGCTGTGA GAAATGGGAG 
TTGATGGAGA GGGTCACCCG CCTGTACAAC GACCAGAAGG ACCTGCAAAA CATGGTGTCT 
AATGCTACAG AAGGCACAGA CACTGGCTCT GGGACGGCCG TGGAGGAGAA CCTCTGTAAG 
ATCTGTATGG ACTCACCCAT CGACTGTGTG CTTCTGGAGT GCGGACACAT GGTCACGTGC 
ACAAAGTGTG GCAAACGCAT GAGCGAATGT CCCATCTGCC GACAGTACGT GGTGCGGGCA 
GTGCACGTGT TCAGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rffl ( XM_009291450.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009289725.1 Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40821
Clone ID Related Accession (Same CDS sequence)	XM_009291450.2 , XM_009291450.3
Accession Version	XM_009291450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase rififylin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGTTTTCA GACTCAGAGG AAGTTTTGGC TCCTGGCCGA 
CGGGCGTCTC TCTCAGACCT AGGATCAGTG GAGGATATTG AGGATTTAAG TGTCCGGCAG 
TTGAAGGAGA TTCTAGCCCG AAACTTTGTC GACTACAAGG GCTGCTGTGA GAAATGGGAG 
TTGATGGAGA GGGTCACCCG CCTGTACAAC GACCAGAAGG ACCTGCAAAA CATGGTGTCT 
AATGCTACAG AAGGCACAGA CACTGGCTCT GGGACGGCCG TGGAGGAGAA CCTCTGTAAG 
ATCTGTATGG ACTCACCCAT CGACTGTGTG CTTCTGGAGT GCGGACACAT GGTCACGTGC 
ACAAAGTGTG GCAAACGCAT GAGCGAATGT CCCATCTGCC GACAGTACGT GGTGCGGGCA 
GTGCACGTGT TCAGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289725.1
CDS	314..1354
Translation	MWASCCGWLCLDDSSMEDTRGSQQRQAFTNSGFSSYPSPTAPEHTCKSCGGRFDSLTRKHVCMDCKKSYCSQCSTQQELQPWLCRTCQRFRGILLERAELMKLKVKDLRDYLHLHGVPTQMCREKEELVELVLGQQTPTSDTHIPAPETTRLDQQETLDTPVITVSPSGNNNTTELGPQTQAMDNGQSLAEVTAESEMDTELQMEQEQFSDSEEVLAPGRRASLSDLGSVEDIEDLSVRQLKEILARNFVDYKGCCEKWELMERVTRLYNDQKDLQNMVSNATEGTDTGSGTAVEENLCKICMDSPIDCVLLECGHMVTCTKCGKRMSECPICRQYVVRAVHVFRS

Target ORF information:

RefSeq Version	XM_009291450.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291450.3

ORF Insert Sequence:

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGTTTTCA GACTCAGAGG AAGTTTTGGC TCCTGGCCGA 
CGGGCGTCTC TCTCAGACCT AGGATCAGTG GAGGATATTG AGGATTTAAG TGTCCGGCAG 
TTGAAGGAGA TTCTAGCCCG AAACTTTGTC GACTACAAGG GCTGCTGTGA GAAATGGGAG 
TTGATGGAGA GGGTCACCCG CCTGTACAAC GACCAGAAGG ACCTGCAAAA CATGGTGTCT 
AATGCTACAG AAGGCACAGA CACTGGCTCT GGGACGGCCG TGGAGGAGAA CCTCTGTAAG 
ATCTGTATGG ACTCACCCAT CGACTGTGTG CTTCTGGAGT GCGGACACAT GGTCACGTGC 
ACAAAGTGTG GCAAACGCAT GAGCGAATGT CCCATCTGCC GACAGTACGT GGTGCGGGCA 
GTGCACGTGT TCAGATCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rffl ( XM_021481368.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337043.1 Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa148936
Clone ID Related Accession (Same CDS sequence)	XM_021481368.1
Accession Version	XM_021481368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase rififylin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGGTAACT CCTGAAACTC CTCTCCTCCT CCTCCTCCTC 
TTCGTCTTTC TGCTGCTCTC TGGCCTGTTC AATGGGGCAG AGGAAAGAAC AGTCAATTCT 
GGCCATCTTT CTGTGAATCA ATATTGCTAT TACTATTATT TATTTACTTA TCTTTTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337043.1
CDS	326..1108
Translation	MWASCCGWLCLDDSSMEDTRGSQQRQAFTNSGFSSYPSPTAPEHTCKSCGGRFDSLTRKHVCMDCKKSYCSQCSTQQELQPWLCRTCQRFRGILLERAELMKLKVKDLRDYLHLHGVPTQMCREKEELVELVLGQQTPTSDTHIPAPETTRLDQQETLDTPVITVSPSGNNNTTELGPQTQAMDNGQSLAEVTAESEMDTELQMEQEQVTPETPLLLLLLFVFLLLSGLFNGAEERTVNSGHLSVNQYCYYYYLFTYLLH

Target ORF information:

RefSeq Version	XM_021481368.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ring finger and FYVE-like domain containing E3 ubiquitin protein ligase (rffl), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481368.1

ORF Insert Sequence:

ATGTGGGCCA GCTGCTGCGG CTGGCTATGT CTGGATGATT CCAGTATGGA AGACACCAGA 
GGAAGCCAAC AGCGCCAGGC CTTCACGAAC TCAGGCTTCA GCAGTTACCC GTCTCCCACT 
GCACCAGAAC ACACATGCAA GTCCTGTGGA GGTCGCTTCG ACAGTTTAAC CAGGAAGCAC 
GTGTGCATGG ACTGCAAGAA GAGCTACTGC AGCCAGTGCT CCACTCAGCA GGAGCTCCAG 
CCGTGGCTGT GCCGCACCTG CCAGCGTTTC CGCGGCATCT TGTTGGAGCG AGCAGAACTC 
ATGAAGCTCA AGGTGAAGGA TCTCAGGGAC TACCTTCACC TGCACGGCGT CCCGACGCAG 
ATGTGCCGTG AGAAGGAGGA GCTGGTCGAG CTGGTTCTCG GCCAGCAGAC GCCCACGTCT 
GACACGCACA TTCCAGCCCC AGAAACCACA CGCTTAGACC AGCAGGAGAC CCTGGACACT 
CCCGTCATTA CAGTGAGCCC GTCAGGGAAC AACAACACAA CAGAACTGGG ACCACAAACA 
CAAGCCATGG ACAATGGACA GAGCTTGGCT GAGGTGACAG CTGAGAGCGA GATGGACACT 
GAGCTGCAGA TGGAGCAGGA GCAGGTAACT CCTGAAACTC CTCTCCTCCT CCTCCTCCTC 
TTCGTCTTTC TGCTGCTCTC TGGCCTGTTC AATGGGGCAG AGGAAAGAAC AGTCAATTCT 
GGCCATCTTT CTGTGAATCA ATATTGCTAT TACTATTATT TATTTACTTA TCTTTTGCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Differential recruitment of co-regulatory proteins to the human estrogen receptor 1 in response to xenoestrogens.
Comparative biochemistry and physiology. Part D, Genomics & proteomics19159-173(2016 Sep)
Smith LC,Clark JC,Bisesi JH Jr,Ferguson PL,Sabo-Attwood T

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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rffl cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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