MID2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MID2
Entrez Gene ID	11043
Full Name	midline 2
Synonyms	FXY2,MRX101,RNF60,TRIM1
General protein information	Preferred Names midline 2 Names probable E3 ubiquitin-protein ligase MID2 RING finger protein 60 RING-type E3 ubiquitin transferase MID2 midin 2 midline defect 2 tripartite motif protein 1 tripartite motif-containing protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.3
Summary	The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Feb 2009].
Disorder MIM:	300204
Disorder Html:	?Mental retardation, X-linked 101, 300928 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_012216.4	NP_036348.2	probable E3 ubiquitin-protein ligase MID2 isoform 1
NM_012216.3	NP_036348.2	probable E3 ubiquitin-protein ligase MID2 isoform 1
XM_005262062.5	XP_005262119.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
XM_005262062.4	XP_005262119.1	probable E3 ubiquitin-protein ligase MID2 isoform X1
NM_052817.3	NP_438112.2	probable E3 ubiquitin-protein ligase MID2 isoform 2
NM_052817.2	NP_438112.2	probable E3 ubiquitin-protein ligase MID2 isoform 2
XM_017029239.1	XP_016884728.1	probable E3 ubiquitin-protein ligase MID2 isoform X2
XM_017029239.2	XP_016884728.1	probable E3 ubiquitin-protein ligase MID2 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MID2	XP_521213.3
Gallus gallus (chicken)	MID2	XP_004940642.1
Rattus norvegicus (Norway rat)	Mid2	NP_001178818.1
Homo sapiens (human)	MID2	NP_036348.2
Canis lupus familiaris (dog)	MID2	XP_005641704.1
Mus musculus (house mouse)	Mid2	NP_035975.1
Xenopus tropicalis (tropical clawed frog)	LOC100497646	XP_002936823.1

Gene Ontology
Bibliography

Related articles in PubMed

Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro and in vivo.
Wang L, Wu J, Yuan J, Zhu X, Wu H, Li M
Frontiers of medicine10(1)41-51(2016 Mar)

The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division.
Gholkar AA, Senese S, Lo YC, Vides E, Contreras E, Hodara E, Capri J, Whitelegge JP, Torres JZ
Cell reports14(2)180-8(2016 Jan)

TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.
Kimura T, Jain A, Choi SW, Mandell MA, Schroder K, Johansen T, Deretic V
The Journal of cell biology210(6)973-89(2015 Sep)

Targeted deep resequencing identifies MID2 mutation for X-linked intellectual disability with varied disease severity in a large kindred from India.
Geetha TS, Michealraj KA, Kabra M, Kaur G, Juyal RC, Thelma BK
Human mutation35(1)41-4(2014 Jan)

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
Uchil PD, Hinz A, Siegel S, Coenen-Stass A, Pertel T, Luban J, Mothes W
Journal of virology87(1)257-72(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpressed in advanced breast cancer and high overexpression is prognostic factor for poor overall survival
Title: Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro and in vivo.

Mid2 regulates cell division through the ubiquitination of astrin on K409, which is critical for its degradation and proper cytokinesis.
Title: The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division.

A novel missense mutation (c.1040G>A, p.Arg347Gln) was reported in MID2, which encodes ubiquitin ligase E3, as the likely cause of X-linked mental retardation in a large kindred.
Title: Targeted deep resequencing identifies MID2 mutation for X-linked intellectual disability with varied disease severity in a large kindred from India.

MID2 is a candidate gene for FG syndrome.
Title: An Xq22.3 duplication detected by comparative genomic hybridization microarray (Array-CGH) defines a new locus (FGS5) for FG syndrome.

MID2 coiled-coil motifs mediate both homo- and heterodimerization, a prerequisite for association of the MID-alpha 4 complex with microtubules.
Title: MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders.

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu99133	XM_017029239.2 Latest version!	Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
OHu12889	NM_052817.3 Latest version!	Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu22054	NM_012216.4 Latest version!	Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu54114	XM_005262062.5 Latest version!	Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu22054	NM_012216.3	Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu54114	XM_005262062.4	Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu12889	NM_052817.2	Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu99133	XM_017029239.1	Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	10-12	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MID2 ( XM_017029239.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884728.1 Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99133
Clone ID Related Accession (Same CDS sequence)	XM_017029239.1 , XM_017029239.2
Accession Version	XM_017029239.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884728.1
CDS	4897..6954
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_017029239.1
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029239.1

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_017029239.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016884728.1 Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99133
Clone ID Related Accession (Same CDS sequence)	XM_017029239.1 , XM_017029239.2
Accession Version	XM_017029239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016884728.1
CDS	5405..7462
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_017029239.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029239.2

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( NM_052817.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_438112.2 Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

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CloneID	OHu12889
Clone ID Related Accession (Same CDS sequence)	NM_052817.3 , NM_052817.2
Accession Version	NM_052817.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, AF196481.1 and AL034399.6. On Jun 2, 2019 this sequence version replaced NM_052817.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.50127.1, SRR1660809.206126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_438112.2
CDS	562..2679
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	NM_052817.3
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052817.3

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( NM_052817.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_438112.2 Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

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CloneID	OHu12889
Clone ID Related Accession (Same CDS sequence)	NM_052817.3 , NM_052817.2
Accession Version	NM_052817.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, AF196481.1 and AL034399.6. On Feb 20, 2009 this sequence version replaced NM_052817.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.50127.1, SRR1660805.103034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_438112.2
CDS	574..2691
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	NM_052817.2
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_052817.2

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT 
CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC 
TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA 
ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC 
AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG 
AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC 
CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA 
TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC 
AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC 
CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA 
GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA 
TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT 
GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG 
ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( NM_012216.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_036348.2 Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.

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CloneID	OHu22054
Clone ID Related Accession (Same CDS sequence)	NM_012216.4 , NM_012216.3
Accession Version	NM_012216.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, Y18880.1 and AL034399.6. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_012216.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85252.1, Y18880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036348.2
CDS	562..2769
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	NM_012216.4
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012216.4

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( NM_012216.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_036348.2 Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.

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CloneID	OHu22054
Clone ID Related Accession (Same CDS sequence)	NM_012216.4 , NM_012216.3
Accession Version	NM_012216.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, Y18880.1 and AL034399.6. This sequence is a reference standard in the RefSeqGene project. On Feb 20, 2009 this sequence version replaced NM_012216.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85252.1, Y18880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036348.2
CDS	574..2781
Translation	MGESPASVVLNASGGLFSLKMETLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	NM_012216.3
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012216.3

ORF Insert Sequence:

ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_005262062.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262119.1 Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.

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CloneID	OHu54114
Clone ID Related Accession (Same CDS sequence)	XM_005262062.5 , XM_005262062.4
Accession Version	XM_005262062.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262062.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262119.1
CDS	5405..7552
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_005262062.5
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262062.5

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MID2 ( XM_005262062.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005262119.1 Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.

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CloneID	OHu54114
Clone ID Related Accession (Same CDS sequence)	XM_005262062.5 , XM_005262062.4
Accession Version	XM_005262062.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	probable E3 ubiquitin-protein ligase MID2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005262062.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005262119.1
CDS	4897..7044
Translation	METLESELTCPICLELFEDPLLLPCAHSLCFSCAHRILVSSCSSGESIEPITAFQCPTCRYVISLNHRGLDGLKRNVTLQNIIDRFQKASVSGPNSPSESRRERTYRPTTAMSSERIACQFCEQDPPRDAVKTCITCEVSYCDRCLRATHPNKKPFTSHRLVEPVPDTHLRGITCLDHENEKVNMYCVSDDQLICALCKLVGRHRDHQVASLNDRFEKLKQTLEMNLTNLVKRNSELENQMAKLIQICQQVEVNTAMHEAKLMEECDELVEIIQQRKQMIAVKIKETKVMKLRKLAQQVANCRQCLERSTVLINQAEHILKENDQARFLQSAKNIAERVAMATASSQVLIPDINFNDAFENFALDFSREKKLLEGLDYLTAPNPPSIREELCTASHDTITVHWISDDEFSISSYELQYTIFTGQANFISKSWCSWGLWPEIRKCKEAVSCSRLAGAPRGLYNSVDSWMIVPNIKQNHYTVHGLQSGTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Target ORF information:

RefSeq Version	XM_005262062.4
Organism	Homo sapiens(human)
Definition	Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005262062.4

ORF Insert Sequence:

ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA 
AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG 
TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG 
AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC 
CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA 
TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC 
TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC 
CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT 
GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG 
GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG 
CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA 
ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA 
AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC 
GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT 
AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG 
AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT 
ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA 
AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA 
GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA 
GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA 
TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG 
ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG 
TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG 
CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC 
AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC 
AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA 
AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA 
GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA 
ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG 
AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC 
AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT 
GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT 
GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC 
CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC 
AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Midline2 is overexpressed and a prognostic indicator in human breast cancer and promotes breast cancer cell proliferation in vitro and in vivo.
Frontiers of medicine10(1)41-51(2016 Mar)
Wang L,Wu J,Yuan J,Zhu X,Wu H,Li M

The X-Linked-Intellectual-Disability-Associated Ubiquitin Ligase Mid2 Interacts with Astrin and Regulates Astrin Levels to Promote Cell Division.
Cell reports14(2)180-8(2016 Jan)
Gholkar AA,Senese S,Lo YC,Vides E,Contreras E,Hodara E,Capri J,Whitelegge JP,Torres JZ

TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity.
The Journal of cell biology210(6)973-89(2015 Sep)
Kimura T,Jain A,Choi SW,Mandell MA,Schroder K,Johansen T,Deretic V

Targeted deep resequencing identifies MID2 mutation for X-linked intellectual disability with varied disease severity in a large kindred from India.
Human mutation35(1)41-4(2014 Jan)
Geetha TS,Michealraj KA,Kabra M,Kaur G,Juyal RC,Thelma BK

TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity.
Journal of virology87(1)257-72(2013 Jan)
Uchil PD,Hinz A,Siegel S,Coenen-Stass A,Pertel T,Luban J,Mothes W

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MID2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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