Mid2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Mid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa07935 NM_001191889.1
Latest version!
Rattus norvegicus midline 2 (Mid2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa25887 XM_006234247.3
Latest version!
Rattus norvegicus midline 2 (Mid2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
ORa25888 XM_006234248.3
Latest version!
Rattus norvegicus midline 2 (Mid2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa07935
    Clone ID Related Accession (Same CDS sequence) NM_001191889.1
    Accession Version NM_001191889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Rattus norvegicus(Norway rat)
    Product probable E3 ubiquitin-protein ligase MID2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AABR07040845.1, AABR07040846.1 and AABR07040847.1. On Jul 17, 2010 this sequence version replaced XM_343825.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA
    AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC
    CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA
    AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT
    GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA
    AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA
    GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG
    GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG
    TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC
    AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA
    ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001178818.1
    CDS165..2372
    Translation

    Target ORF information:

    RefSeq Version NM_001191889.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus midline 2 (Mid2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001191889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA
    AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC
    CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA
    AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT
    GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA
    AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA
    GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG
    GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG
    TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC
    AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA
    ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa25887
    Clone ID Related Accession (Same CDS sequence) XM_006234247.3
    Accession Version XM_006234247.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA
    AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC
    CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA
    AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT
    GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA
    AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA
    GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG
    GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGACAGCTG GATGATTGTG
    CCCAACATTA AGCAGAACCA TTACACAGTC CATGGACTCC AGAGTGGGAC ACGCTACATC
    TTTATTGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GTGAACCCAC CAGGCTAAAA
    ACAAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGACGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG
    AGATTTAGTG GCACGGGGTG TTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CAATCGGTAT TGCCTACAAA
    TCAGCTCCAA AGAACGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC
    AACAGTCACT TCGTGGTGCG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG
    TTGAAGCGTC TAGGCGTCCT CTTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA
    TTCACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCGTA TGTGTCGGGG
    ATGAAAGCTT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006234309.1
    CDS588..2705
    Translation

    Target ORF information:

    RefSeq Version XM_006234247.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus midline 2 (Mid2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006234247.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAGA GCCCAGCCTC TGCGGTTCTT AATGCCTCAG CAGGATTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA
    AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC
    CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA
    AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT
    GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA
    AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA
    GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG
    GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TACAATTCAG TGGACAGCTG GATGATTGTG
    CCCAACATTA AGCAGAACCA TTACACAGTC CATGGACTCC AGAGTGGGAC ACGCTACATC
    TTTATTGTGA AAGCCATAAA TCAAGCAGGC AGCCGGAACA GTGAACCCAC CAGGCTAAAA
    ACAAACAGCC AACCATTTAA ACTAGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGACGAG AGCTCTCTGA AGAAGAGCCA CACTCCGGAG
    AGATTTAGTG GCACGGGGTG TTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTACTGGG AGGTGGTCAT GGGTTCTTCA ACATGGTATG CAATCGGTAT TGCCTACAAA
    TCAGCTCCAA AGAACGAGTG GATTGGCAAG AATGCCTCTT CCTGGGTCTT CTCTCGATGC
    AACAGTCACT TCGTGGTGCG ACATAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAG
    TTGAAGCGTC TAGGCGTCCT CTTGGATTAT GACAACAACA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTTACCTTCA TTCTTCCAGT TTGTCCAACA
    TTCACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTATCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCGTA TGTGTCGGGG
    ATGAAAGCTT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa25888
    Clone ID Related Accession (Same CDS sequence) XM_006234248.3
    Accession Version XM_006234248.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234248.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA
    AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC
    CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA
    AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT
    GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA
    AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA
    GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG
    GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG
    TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC
    AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA
    ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006234310.1
    CDS256..2403
    Translation

    Target ORF information:

    RefSeq Version XM_006234248.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus midline 2 (Mid2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006234248.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TCGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTATGT TTTAGCTGTG CTCACCGCAT CCTGGTCTCA
    AGCTGCAGCT CCGGTGAATC CATTGAACCC ATTACTGCTT TTCAGTGTCC TACATGCAGG
    TATGTTATCT CGCTGAATCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACCCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCTCC AAGTGAGAGC
    CGTCGGGAGA GGACTTACAG GCCTGGTTCC GCCATGTCGA GTGAGCGAAT TGCATGTCAG
    TTCTGTGAGC AGGACCCTCC GAGAGATGCT GTAAAGACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GGTGCCTTCG GGCCACACAC CCCAACAAGA AACCTTTCAC GAGCCATCGC
    CTGGTGGAAC CAGTTTCAGA CACACATCTT CGAGGGATTA CCTGTCTGGA CCATGAGAAT
    GAGAAGGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCT TCTCTGAATG ATCGATTTGA GAAACTAAAG
    CAAACTCTCG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAA GTTGAGGTGA ATACTGCTAT GCACGAGGCA
    AAACTTATGG AAGAGTGTGA CGAGCTGGTA GAGATCATCC AGCAGAGGAA ACAAATGATT
    GCCGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCCCA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAGGCTGA GCATATCCTA
    AAGGAAAACG ACCAAGCTCG CTTTCTGCAG TCTGCAAAAA ATATTGCCGA GAGGGTCGCT
    ATGGCAACTG CATCTTCGCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC TAGAGAAAAG AAACTATTGG AGGGGCTAGA CTATTTAACA
    GCTCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CTTCCCATGA CACCATTACG
    GTCCACTGGA TCTCAGACGA CGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGACTGG CCGGTGCGCC ACGAGGCCTG
    TACAATTCAG TGGACAGCTG GATGATTGTG CCCAACATTA AGCAGAACCA TTACACAGTC
    CATGGACTCC AGAGTGGGAC ACGCTACATC TTTATTGTGA AAGCCATAAA TCAAGCAGGC
    AGCCGGAACA GTGAACCCAC CAGGCTAAAA ACAAACAGCC AACCATTTAA ACTAGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGACGAG
    AGCTCTCTGA AGAAGAGCCA CACTCCGGAG AGATTTAGTG GCACGGGGTG TTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTACTGGG AGGTGGTCAT GGGTTCTTCA
    ACATGGTATG CAATCGGTAT TGCCTACAAA TCAGCTCCAA AGAACGAGTG GATTGGCAAG
    AATGCCTCTT CCTGGGTCTT CTCTCGATGC AACAGTCACT TCGTGGTGCG ACATAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAG TTGAAGCGTC TAGGCGTCCT CTTGGATTAT
    GACAACAACA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTTACCTTCA TTCTTCCAGT TTGTCCAACA TTCACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTATCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCGTA TGTGTCGGGG ATGAAAGCTT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.