MID2 cDNA ORF clone, Homo sapiens(human)

The following MID2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MID2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu99133 XM_017029239.2
Latest version!
Homo sapiens midline 2 (MID2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
OHu12889 NM_052817.3
Latest version!
Homo sapiens midline 2 (MID2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu22054 NM_012216.4
Latest version!
Homo sapiens midline 2 (MID2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu54114 XM_005262062.5
Latest version!
Homo sapiens midline 2 (MID2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
View Old Accession Versions >>
OHu22054 NM_012216.3 Homo sapiens midline 2 (MID2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu54114 XM_005262062.4 Homo sapiens midline 2 (MID2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu12889 NM_052817.2 Homo sapiens midline 2 (MID2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OHu99133 XM_017029239.1 Homo sapiens midline 2 (MID2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu99133
    Clone ID Related Accession (Same CDS sequence) XM_017029239.1 , XM_017029239.2
    Accession Version XM_017029239.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884728.1
    CDS4897..6954
    Translation

    Target ORF information:

    RefSeq Version XM_017029239.1
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu99133
    Clone ID Related Accession (Same CDS sequence) XM_017029239.1 , XM_017029239.2
    Accession Version XM_017029239.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017029239.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016884728.1
    CDS5405..7462
    Translation

    Target ORF information:

    RefSeq Version XM_017029239.2
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017029239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12889
    Clone ID Related Accession (Same CDS sequence) NM_052817.3 , NM_052817.2
    Accession Version NM_052817.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, AF196481.1 and AL034399.6. On Jun 2, 2019 this sequence version replaced NM_052817.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.50127.1, SRR1660809.206126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_438112.2
    CDS562..2679
    Translation

    Target ORF information:

    RefSeq Version NM_052817.3
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_052817.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu12889
    Clone ID Related Accession (Same CDS sequence) NM_052817.3 , NM_052817.2
    Accession Version NM_052817.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-19
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, AF196481.1 and AL034399.6. On Feb 20, 2009 this sequence version replaced NM_052817.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. It encodes isoform 2 which is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.50127.1, SRR1660805.103034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_438112.2
    CDS574..2691
    Translation

    Target ORF information:

    RefSeq Version NM_052817.2
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_052817.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGCCTG TATAATTCAG TAGACAGCTG GATGATTGTT
    CCCAACATTA AACAGAACCA TTACACAGTG CATGGACTCC AGAGCGGGAC TCGCTACATC
    TTCATCGTTA AAGCCATAAA CCAAGCCGGC AGCCGGAACA GTGAACCTAC CCGACTAAAA
    ACAAACAGCC AACCCTTTAA ATTGGATCCC AAAATGACTC ACAAGAAGTT GAAGATCTCC
    AATGATGGAT TGCAGATGGA GAAGGATGAA AGCTCTCTAA AGAAGAGCCA CACCCCAGAG
    AGGTTTAGTG GCACAGGGTG CTATGGGGCA GCAGGAAATA TATTCATTGA CAGTGGCTGC
    CACTATTGGG AGGTGGTCAT GGGTTCCTCA ACATGGTATG CAATTGGCAT TGCCTACAAA
    TCAGCTCCAA AGAATGAATG GATTGGCAAG AATGCCTCCT CATGGGTCTT CTCTCGCTGC
    AATAGTAACT TCGTGGTGAG ACACAACAAC AAGGAAATGC TGGTGGATGT GCCCCCACAC
    CTGAAGCGTC TGGGTGTCCT CCTGGATTAT GACAACAATA TGCTGTCTTT CTATGACCCA
    GCTAACTCTC TCCATCTTCA TACTTTTGAT GTGACCTTCA TTCTTCCAGT TTGTCCAACA
    TTTACAATCT GGAACAAATC CCTAATGATC CTGTCTGGCT TGCCTGCCCC AGATTTTATT
    GATTACCCTG AGCGGCAGGA ATGCAACTGC AGGCCTCAAG AATCCCCTTA TGTTTCTGGG
    ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22054
    Clone ID Related Accession (Same CDS sequence) NM_012216.4 , NM_012216.3
    Accession Version NM_012216.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, Y18880.1 and AL034399.6. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_012216.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85252.1, Y18880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036348.2
    CDS562..2769
    Translation

    Target ORF information:

    RefSeq Version NM_012216.4
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_012216.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22054
    Clone ID Related Accession (Same CDS sequence) NM_012216.4 , NM_012216.3
    Accession Version NM_012216.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997921.1, Y18880.1 and AL034399.6. This sequence is a reference standard in the RefSeqGene project. On Feb 20, 2009 this sequence version replaced NM_012216.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85252.1, Y18880.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_036348.2
    CDS574..2781
    Translation

    Target ORF information:

    RefSeq Version NM_012216.3
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_012216.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGTGAAA GCCCAGCCTC CGTGGTTCTT AATGCCTCAG GAGGACTATT TTCACTAAAG 
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54114
    Clone ID Related Accession (Same CDS sequence) XM_005262062.5 , XM_005262062.4
    Accession Version XM_005262062.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005262062.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262119.1
    CDS5405..7552
    Translation

    Target ORF information:

    RefSeq Version XM_005262062.5
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262062.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu54114
    Clone ID Related Accession (Same CDS sequence) XM_005262062.5 , XM_005262062.4
    Accession Version XM_005262062.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2148bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product probable E3 ubiquitin-protein ligase MID2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005262062.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005262119.1
    CDS4897..7044
    Translation

    Target ORF information:

    RefSeq Version XM_005262062.4
    Organism Homo sapiens(human)
    Definition Homo sapiens midline 2 (MID2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005262062.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAAACAC TGGAGTCTGA ATTGACCTGT CCAATCTGCC TAGAGTTGTT TGAAGACCCC 
    CTTCTGCTCC CTTGTGCTCA CAGCCTCTGC TTCAGCTGTG CCCATCGCAT TTTGGTATCA
    AGCTGCAGCT CTGGTGAATC CATTGAACCC ATTACTGCTT TCCAGTGTCC TACCTGCAGG
    TATGTTATCT CGCTGAACCA CCGGGGCCTG GATGGCCTCA AGAGGAATGT GACTCTGCAG
    AACATTATTG ATCGCTTCCA GAAGGCTTCA GTCAGTGGGC CCAATTCCCC TAGTGAGAGC
    CGCCGGGAAA GGACTTACAG GCCCACCACT GCCATGTCTA GCGAGCGAAT TGCTTGCCAA
    TTCTGTGAGC AGGACCCGCC AAGGGATGCA GTAAAAACAT GCATCACCTG TGAGGTCTCC
    TACTGTGACC GTTGCCTGCG GGCCACGCAC CCCAACAAGA AACCTTTCAC CAGCCACCGC
    CTGGTGGAAC CAGTGCCAGA CACACATCTT CGAGGGATCA CCTGCCTGGA CCATGAGAAT
    GAGAAAGTGA ACATGTACTG TGTATCTGAT GACCAATTGA TCTGTGCCTT ATGCAAACTG
    GTGGGTCGTC ACCGAGACCA TCAGGTCGCA TCCCTGAATG ATCGATTTGA GAAACTCAAG
    CAAACTCTGG AGATGAACCT CACCAACCTG GTTAAGCGCA ACAGCGAACT AGAAAATCAA
    ATGGCCAAAC TAATACAGAT CTGCCAGCAG GTTGAGGTGA ATACTGCTAT GCATGAGGCA
    AAACTTATGG AAGAATGTGA CGAGTTGGTA GAGATCATCC AGCAGAGGAA GCAAATGATC
    GCTGTCAAAA TCAAAGAGAC AAAGGTTATG AAACTGAGAA AGTTGGCACA GCAGGTTGCT
    AATTGCCGCC AGTGTCTTGA ACGGTCAACA GTCCTCATCA ACCAAGCTGA GCATATCCTG
    AAAGAAAATG ACCAGGCACG GTTTCTACAG TCTGCAAAAA ATATTGCTGA GAGGGTCGCT
    ATGGCAACTG CATCTTCTCA AGTTCTGATT CCAGACATCA ATTTTAATGA TGCCTTTGAA
    AACTTTGCTT TAGATTTTTC CAGAGAAAAG AAACTGCTAG AGGGGTTAGA TTATTTAACA
    GCCCCAAACC CACCATCTAT CCGAGAAGAA CTCTGTACTG CCTCCCATGA CACCATTACA
    GTCCACTGGA TCTCGGATGA TGAGTTCAGC ATCAGCTCCT ATGAGCTTCA GTACACCATA
    TTCACTGGCC AGGCTAACTT CATCAGTAAG TCATGGTGTA GTTGGGGCCT GTGGCCAGAG
    ATAAGGAAAT GTAAGGAAGC AGTAAGCTGC TCAAGATTGG CCGGGGCGCC ACGAGGCCTG
    TATAATTCAG TAGACAGCTG GATGATTGTT CCCAACATTA AACAGAACCA TTACACAGTG
    CATGGACTCC AGAGCGGGAC TCGCTACATC TTCATCGTTA AAGCCATAAA CCAAGCCGGC
    AGCCGGAACA GTGAACCTAC CCGACTAAAA ACAAACAGCC AACCCTTTAA ATTGGATCCC
    AAAATGACTC ACAAGAAGTT GAAGATCTCC AATGATGGAT TGCAGATGGA GAAGGATGAA
    AGCTCTCTAA AGAAGAGCCA CACCCCAGAG AGGTTTAGTG GCACAGGGTG CTATGGGGCA
    GCAGGAAATA TATTCATTGA CAGTGGCTGC CACTATTGGG AGGTGGTCAT GGGTTCCTCA
    ACATGGTATG CAATTGGCAT TGCCTACAAA TCAGCTCCAA AGAATGAATG GATTGGCAAG
    AATGCCTCCT CATGGGTCTT CTCTCGCTGC AATAGTAACT TCGTGGTGAG ACACAACAAC
    AAGGAAATGC TGGTGGATGT GCCCCCACAC CTGAAGCGTC TGGGTGTCCT CCTGGATTAT
    GACAACAATA TGCTGTCTTT CTATGACCCA GCTAACTCTC TCCATCTTCA TACTTTTGAT
    GTGACCTTCA TTCTTCCAGT TTGTCCAACA TTTACAATCT GGAACAAATC CCTAATGATC
    CTGTCTGGCT TGCCTGCCCC AGATTTTATT GATTACCCTG AGCGGCAGGA ATGCAACTGC
    AGGCCTCAAG AATCCCCTTA TGTTTCTGGG ATGAAAACCT GTCATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.