mid2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mid2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mid2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa14272 XM_018096931.2
Latest version!
Xenopus tropicalis midline 2 (mid2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa14272 XM_018096932.2
Latest version!
Xenopus tropicalis midline 2 (mid2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OXa14272 XM_018096931.1 Xenopus tropicalis midline 2 (mid2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa14272 XM_018096932.1 Xenopus tropicalis midline 2 (mid2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa14272
    Clone ID Related Accession (Same CDS sequence) XM_018096931.1 , XM_018096932.2 , XM_018096931.2 , XM_018096932.1
    Accession Version XM_018096931.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase MID2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952420.1
    CDS938..3001
    Translation

    Target ORF information:

    RefSeq Version XM_018096931.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis midline 2 (mid2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14272
    Clone ID Related Accession (Same CDS sequence) XM_018096931.1 , XM_018096932.2 , XM_018096931.2 , XM_018096932.1
    Accession Version XM_018096932.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase MID2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952421.1
    CDS252..2315
    Translation

    Target ORF information:

    RefSeq Version XM_018096932.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis midline 2 (mid2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096932.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14272
    Clone ID Related Accession (Same CDS sequence) XM_018096931.1 , XM_018096932.2 , XM_018096931.2 , XM_018096932.1
    Accession Version XM_018096932.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase MID2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096932.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952421.1
    CDS382..2445
    Translation

    Target ORF information:

    RefSeq Version XM_018096932.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis midline 2 (mid2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096932.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14272
    Clone ID Related Accession (Same CDS sequence) XM_018096931.1 , XM_018096932.2 , XM_018096931.2 , XM_018096932.1
    Accession Version XM_018096931.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2064bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product probable E3 ubiquitin-protein ligase MID2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017952420.1
    CDS963..3026
    Translation

    Target ORF information:

    RefSeq Version XM_018096931.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis midline 2 (mid2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018096931.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGGAAACAC TGGAGTCAGA ACTGACCTGT CCAATCTGCC TGGAGTTATT TGAGGACCCG 
    CTGCTTCTCC CTTGTGCCCA TAGCTTGTGC TTCAGCTGTG CCCACCGTAT TCTCACTTCC
    GGATGTGCTT CCAACGAAAT AGCGGAGCCA GTACCAGCTT TTCAGTGTCC GACTTGCAGA
    TATGTTATAT CTTTAAATCA CCGCGGTCTG GAAGGTTTAA AACGCAACGT TACCTTGCAG
    AACATTATTG ATCGCTTTCA GAAGGCTTCT TTAAGTGTTC CCAACTCTCC TAGTGACGAT
    CACAGGGAAA AAATCTATAG GACTGTCCCC ATTATGGCCT CCGAAAGGAT CCCGTGCCAG
    TTCTGTGAGA CAGAGCCACC AAGGGATGCC GTGAAAACAT GTATCACCTG TGAGGTGTCC
    TACTGTGATC GGTGTTTGAG GGCAACTCAC CCCAATAAAA AGCCCTTTAC CAGCCACAGA
    CTGGTGGAAC CAATTCCAGA CGGTCACTTT CGGGGCCTGG CTTGTCTAGA ACATGAAAAT
    GAGAAAGTCA ATATGTACTG TACAGTAGAC GATCAGCTTA TCTGTGCCTT ATGCAAACTG
    GTTGGTAGGC ACAGAGAGCA CCAGGTGGCT TCTTTAAGTG ACAGATATGA AAAACTCAAG
    CAAACCTTGG AATCCAATTT TCCCATCTTG GTCAAGCACA GCAGTGAACT TGAAAGCCAG
    ATGACCAAAC TCATCCAGAT TTGCCAACAG GTTGAGGTAA ACACCAGCAT GCATGAAGCA
    AAACTGAATG AAGAATGTGA CGAGCTAATG GACATCATAC AGAGAAGAAA GCAAATGATA
    GCAGTGAAAA TCAAAGAGAC AAAAGTCATG AAGTTAAGAA AACTTGCTCA ACAAATTGCC
    AATTGTCGTC AACGTCTGGA ACGATCAACA GTACTAATCA ACCAAACAGA TCATATACTC
    AAGGAGAGCG ATCATGCAAG GTTTCTGCAG GGAGCTAAAA CTGTAGCAGA GAGAATCTCC
    ATGACAGCAA CCTCCTCTCA TGTTCTAATG CCTGATGTCA ATTTGAACGA TGCCTTTGAA
    AATTTTGCTT TGGATTTCTC CAGAGAGAAG AAATTGCTTG AAGGTCTGGA CTATGTAACT
    GCTCCCAACC CTCCATCCAT CAGAGAGGAG CTGTGCACTG CATCCCATGA CACCATAACT
    GTCCACTGGA CATCAGATGA TGAATTCAGT GTGGGCTCCT ATGAGCTCCA GTACACCATT
    TATACTGGGC AAGCCAACTT CATCAGTCTG TACAGTTCCA TGGACAGCTG GATGATTGTA
    CCTAACATCA AGCAGAACCA TTACACTGTG CATGGGCTCC AGAGTGGCAC ACGATACATC
    TTCCTGGTCA AAGCAATTAA CCAGGCTGGA AGCAGGCACA GTGATCCTGC AAGGCTGAAG
    ACTAACAGCC AGCCATTTAA GCTGGATCCT AAATATGCTC ACAAGAAGCT GAAAATATCC
    AATGATGGAA TGCAGATGGA GAAGGAGGAG AGCTCCCTAA AGAAGAGCCA CACACCTGAG
    AGGTTCAGCG GAACTGGATG TTATGGCGCA ACAGGGAATG TCTTTATTGA CAGTGGATGC
    CACTACTGGG AGGTGGCTGT AGGAAATTCC ACTTGGTATG CAATAGGAAT CGCCTATAAA
    TCCGCTCCAA GGAATGAGTG GGTTGGGAAG AATACCTCCT CGTGGGTCTT CACTCGATGC
    AACAATAATT TTCTTGTAAG ACACAATAAC AAAGAAGCTC TTCTGGATGT GAATCCTCAG
    TTGAAACGTT TAGGTGTTCT CCTAGACTAT GATAATAATA CACTTTCCTT CTATGATCCA
    GCCAACTCCC TCCACCTGCA CACCTTCCCC ATCTCCTTTA TTCTCCCTGT GTGCCCAACC
    TTCACTATAT GGAACAAGTC TGTGTTGATA CTCTCAGGAC TCCCTGCTCC GGATTTTATG
    GACTCCTTTA TGTCTCAGCC GCAAGAAACT ATATCCAGAC CTCAGGAGTC TCCCTATGTT
    TCTGGGCTTA AGGGTTGCCA GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.