CFL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CFL2
Entrez Gene ID	1073
Full Name	cofilin 2
Synonyms	NEM7
General protein information	Preferred Names cofilin 2 Names cofilin-2 cofilin 2 (muscle) cofilin, muscle isoform nemaline myopathy type 7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q13.1
Summary	This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2009].
Disorder MIM:	601443
Disorder Html:	Nemaline myopathy 7, autosomal recessive, 610687 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011536363.2	XP_011534665.1	cofilin-2 isoform X1
XM_011536363.3	XP_011534665.1	cofilin-2 isoform X1
NM_001243645.1	NP_001230574.1	cofilin-2 isoform 2
NM_021914.7	NP_068733.1	cofilin-2 isoform 1
NM_138638.4	NP_619579.1	cofilin-2 isoform 1
XM_011536363.1	XP_011534665.1	cofilin-2 isoform X1
NM_138638.5	NP_619579.1	cofilin-2 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	CFL2	NP_619579.1
	CFL2	XP_001082579.2
Canis lupus familiaris (dog)	CFL2	XP_547771.2
Gallus gallus (chicken)	CFL2	NP_001004406.1
Saccharomyces cerevisiae (baker's yeast)	COF1	NP_013050.1
Magnaporthe oryzae (rice blast fungus)	MGG_03164	XP_003716827.1
Arabidopsis thaliana (thale cress)	ADF6	NP_565719.1
Pan troglodytes (chimpanzee)	CFL2	XP_509898.2
Mus musculus (house mouse)	Cfl2	NP_031714.1
Neurospora crassa	NCU01587	XP_956498.2
Bos taurus (cattle)	CFL2	NP_001069622.1
Rattus norvegicus (Norway rat)	Cfl2	NP_001102452.2
Kluyveromyces lactis	KLLA0E00463g	XP_453967.1
Eremothecium gossypii	AGOS_ADR235W	NP_984331.1
Schizosaccharomyces pombe (fission yeast)	adf1	NP_594741.1
Oryza sativa (rice)	Os03g0780400	NP_001051449.1
Xenopus tropicalis (tropical clawed frog)	cfl2	NP_001011156.1
Danio rerio (zebrafish)	cfl2	NP_991263.1
Oryza sativa (rice)	Os12g0628100	NP_001067327.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-3189-3p Mimics Enhance the Effects of S100A4 siRNA on the Inhibition of Proliferation and Migration of Gastric Cancer Cells by Targeting CFL2.
Bian Y, Guo J, Qiao L, Sun X
International journal of molecular sciences19(1)(2018 Jan)

Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.
Yehl J, Kudryashova E, Reisler E, Kudryashov D, Polenova T
Scientific reports744506(2017 Mar)

Changes in the Level of Circulating hsa-miR-297 and hsa-miR-19b-3p miRNA Are Associated with Generalization of Prostate Cancer.
Osip'yants AI, Knyazev EN, Galatenko AV, Nyushko KM, Galatenko VV, Shkurnikov MY, Alekseev BY
Bulletin of experimental biology and medicine162(3)379-382(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The greatest levels of circulating miR-297 and miR-19b-3p with its common target CFL2 are associated with metastatic prostate cancer.
Title: Changes in the Level of Circulating hsa-miR-297 and hsa-miR-19b-3p miRNA Are Associated with Generalization of Prostate Cancer.

analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover
Title: TIRF microscopy analysis of human Cof1, Cof2, and ADF effects on actin filament severing and turnover.

In primary tumours, both desmin and CFL2 expression predicted improved overall survival in multivariate analyses
Title: Progressive loss of myogenic differentiation in leiomyosarcoma has prognostic value.

Cofilin 2 phosphorylation and genetic overexpression plays a role in the pathogenesis of idiopathic dilated cardiomyopthay.
Title: Cofilin-2 phosphorylation and sequestration in myocardial aggregates: novel pathogenetic mechanisms for idiopathic dilated cardiomyopathy.

The serum lever of Alzheimer's disease were increase and the expression of clf2 strongly correlated with the Mini-Mental State Examination scores of the AD patients
Title: Translational study of Alzheimer's disease (AD) biomarkers from brain tissues in A?PP/PS1 mice and serum of AD patients.

The following CFL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CFL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22052	NM_001243645.1 Latest version!	Homo sapiens cofilin 2 (CFL2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22099	NM_138638.5 Latest version!	Homo sapiens cofilin 2 (CFL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22099	NM_021914.7 Latest version!	Homo sapiens cofilin 2 (CFL2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22052	XM_011536363.3 Latest version!	Homo sapiens cofilin 2 (CFL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22052	XM_011536363.2	Homo sapiens cofilin 2 (CFL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22052	XM_011536363.1	Homo sapiens cofilin 2 (muscle) (CFL2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22099	NM_138638.4	Homo sapiens cofilin 2 (CFL2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CFL2 ( NM_001243645.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230574.1 Homo sapiens cofilin 2 (CFL2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22052
Clone ID Related Accession (Same CDS sequence)	XM_011536363.2 , XM_011536363.3 , NM_001243645.1 , XM_011536363.1
Accession Version	NM_001243645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cofilin-2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL599958.1, DA298739.1 and AL355885.4. On Aug 19, 2011 this sequence version replaced NR_028132.1. Transcript Variant: This variant (5) differs in the 5' UTR and uses a downstream, in-frame start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.107063.1, SRR1803616.2726.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230574.1
CDS	170..619
Translation	MKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	NM_001243645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cofilin 2 (CFL2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243645.1

ORF Insert Sequence:

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFL2 ( NM_021914.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_068733.1 Homo sapiens cofilin 2 (CFL2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22099
Clone ID Related Accession (Same CDS sequence)	NM_021914.7 , NM_138638.4 , NM_138638.5
Accession Version	NM_021914.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	cofilin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF134802.1, AL355885.4 and DB378917.1. On Jul 25, 2009 this sequence version replaced NM_021914.6. Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate translational start codon, compared to variant 2, but the encoded isoform (1) is identical to that encoded by variant 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF134802.1, SRR1660809.110168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGGAAATCTT CTACACAAGA GGAGATCAAA AAGAGAAAGA AAGCAGTTCT CTTCTGTTTA 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCAAAGC AGATCTTGGT GGGTGACATT 
GGTGATACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGCTACCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACATACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCT TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GATGATATTA AGGACCGTTC GACACTTGGA GAGAAATTGG GAGGCAATGT AGTAGTTTCA 
CTTGAAGGAA AACCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_068733.1
CDS	142..642
Translation	MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	NM_021914.7
Organism	Homo sapiens(human)
Definition	Homo sapiens cofilin 2 (CFL2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021914.7

ORF Insert Sequence:

ATGGCTTCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGGAAATCTT CTACACAAGA GGAGATCAAA AAGAGAAAGA AAGCAGTTCT CTTCTGTTTA 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCAAAGC AGATCTTGGT GGGTGACATT 
GGTGATACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGCTACCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACATACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCT TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GATGATATTA AGGACCGTTC GACACTTGGA GAGAAATTGG GAGGCAATGT AGTAGTTTCA 
CTTGAAGGAA AACCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFL2 ( NM_138638.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_619579.1 Homo sapiens cofilin 2 (CFL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22099
Clone ID Related Accession (Same CDS sequence)	NM_021914.7 , NM_138638.4 , NM_138638.5
Accession Version	NM_138638.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	cofilin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC025683.1, AL355885.4, BC022364.1 and DB378917.1. This sequence is a reference standard in the RefSeqGene project. On Jul 25, 2009 this sequence version replaced NM_138638.3. Transcript Variant: This variant (2) encodes the functional protein. Both variants 1 and 2 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.65122.1, SRR1660807.96079.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGGAAATCTT CTACACAAGA GGAGATCAAA AAGAGAAAGA AAGCAGTTCT CTTCTGTTTA 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCAAAGC AGATCTTGGT GGGTGACATT 
GGTGATACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGCTACCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACATACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCT TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GATGATATTA AGGACCGTTC GACACTTGGA GAGAAATTGG GAGGCAATGT AGTAGTTTCA 
CTTGAAGGAA AACCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619579.1
CDS	284..784
Translation	MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	NM_138638.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cofilin 2 (CFL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138638.4

ORF Insert Sequence:

ATGGCTTCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGGAAATCTT CTACACAAGA GGAGATCAAA AAGAGAAAGA AAGCAGTTCT CTTCTGTTTA 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCAAAGC AGATCTTGGT GGGTGACATT 
GGTGATACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGCTACCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACATACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCT TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GATGATATTA AGGACCGTTC GACACTTGGA GAGAAATTGG GAGGCAATGT AGTAGTTTCA 
CTTGAAGGAA AACCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFL2 ( NM_138638.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_619579.1 Homo sapiens cofilin 2 (CFL2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22099
Clone ID Related Accession (Same CDS sequence)	NM_021914.7 , NM_138638.4 , NM_138638.5
Accession Version	NM_138638.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	cofilin-2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC022364.1, AL355885.4 and DB378917.1. On May 16, 2019 this sequence version replaced NM_138638.4. Transcript Variant: This variant (2) encodes the longer isoform (1). Both variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.187536.1, SRR1660805.220230.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000298159.11/ ENSP00000298159.6 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTTCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGGAAATCTT CTACACAAGA GGAGATCAAA AAGAGAAAGA AAGCAGTTCT CTTCTGTTTA 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCAAAGC AGATCTTGGT GGGTGACATT 
GGTGATACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGCTACCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACATACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCT TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GATGATATTA AGGACCGTTC GACACTTGGA GAGAAATTGG GAGGCAATGT AGTAGTTTCA 
CTTGAAGGAA AACCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_619579.1
CDS	54..554
Translation	MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	NM_138638.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cofilin 2 (CFL2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138638.5

ORF Insert Sequence:

ATGGCTTCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGGAAATCTT CTACACAAGA GGAGATCAAA AAGAGAAAGA AAGCAGTTCT CTTCTGTTTA 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCAAAGC AGATCTTGGT GGGTGACATT 
GGTGATACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGCTACCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACATACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCT TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GATGATATTA AGGACCGTTC GACACTTGGA GAGAAATTGG GAGGCAATGT AGTAGTTTCA 
CTTGAAGGAA AACCATTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFL2 ( XM_011536363.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534665.1 Homo sapiens cofilin 2 (CFL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22052
Clone ID Related Accession (Same CDS sequence)	XM_011536363.2 , XM_011536363.3 , NM_001243645.1 , XM_011536363.1
Accession Version	XM_011536363.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	cofilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011536363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534665.1
CDS	131..580
Translation	MKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	XM_011536363.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cofilin 2 (CFL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536363.2

ORF Insert Sequence:

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFL2 ( XM_011536363.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534665.1 Homo sapiens cofilin 2 (CFL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22052
Clone ID Related Accession (Same CDS sequence)	XM_011536363.2 , XM_011536363.3 , NM_001243645.1 , XM_011536363.1
Accession Version	XM_011536363.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	cofilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000014.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011536363.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534665.1
CDS	90..539
Translation	MKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	XM_011536363.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cofilin 2 (CFL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536363.3

ORF Insert Sequence:

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CFL2 ( XM_011536363.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534665.1 Homo sapiens cofilin 2 (muscle) (CFL2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu22052
Clone ID Related Accession (Same CDS sequence)	XM_011536363.2 , XM_011536363.3 , NM_001243645.1 , XM_011536363.1
Accession Version	XM_011536363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	cofilin-2 isoform X1
Comment

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534665.1
Translation	MKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Target ORF information:

RefSeq Version	XM_011536363.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cofilin 2 (muscle) (CFL2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011536363.1

ORF Insert Sequence:

ATGAAAGTAA GGAAATCTTC TACACAAGAG GAGATCAAAA AGAGAAAGAA AGCAGTTCTC 
TTCTGTTTAA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCAAAGCA GATCTTGGTG 
GGTGACATTG GTGATACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GCTACCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACATACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCTT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ATGATATTAA GGACCGTTCG ACACTTGGAG AGAAATTGGG AGGCAATGTA 
GTAGTTTCAC TTGAAGGAAA ACCATTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-3189-3p Mimics Enhance the Effects of S100A4 siRNA on the Inhibition of Proliferation and Migration of Gastric Cancer Cells by Targeting CFL2.
International journal of molecular sciences19(1)(2018 Jan)
Bian Y,Guo J,Qiao L,Sun X

Structural Analysis of Human Cofilin 2/Filamentous Actin Assemblies: Atomic-Resolution Insights from Magic Angle Spinning NMR Spectroscopy.
Scientific reports744506(2017 Mar)
Yehl J,Kudryashova E,Reisler E,Kudryashov D,Polenova T

Changes in the Level of Circulating hsa-miR-297 and hsa-miR-19b-3p miRNA Are Associated with Generalization of Prostate Cancer.
Bulletin of experimental biology and medicine162(3)379-382(2017 Jan)
Osip'yants AI,Knyazev EN,Galatenko AV,Nyushko KM,Galatenko VV,Shkurnikov MY,Alekseev BY

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CFL2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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