Cfl2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cfl2
Entrez Gene ID	12632
Full Name	cofilin 2, muscle
General protein information	Preferred Names cofilin 2, muscle Names cofilin-2 cofilin, muscle isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12\|12 C1

mRNA and Protein(s)

mRNA	Protein	Name
XM_006515452.3	XP_006515515.1	cofilin-2 isoform X1
XM_006515452.2	XP_006515515.1	cofilin-2 isoform X1
NM_007688.2	NP_031714.1	cofilin-2

Pathways from BioSystems

Homologies

Homo sapiens (human)	CFL2	NP_619579.1
	CFL2	XP_001082579.2
Canis lupus familiaris (dog)	CFL2	XP_547771.2
Gallus gallus (chicken)	CFL2	NP_001004406.1
Saccharomyces cerevisiae (baker's yeast)	COF1	NP_013050.1
Magnaporthe oryzae (rice blast fungus)	MGG_03164	XP_003716827.1
Arabidopsis thaliana (thale cress)	ADF6	NP_565719.1
Pan troglodytes (chimpanzee)	CFL2	XP_509898.2
Mus musculus (house mouse)	Cfl2	NP_031714.1
Neurospora crassa	NCU01587	XP_956498.2
Bos taurus (cattle)	CFL2	NP_001069622.1
Rattus norvegicus (Norway rat)	Cfl2	NP_001102452.2
Kluyveromyces lactis	KLLA0E00463g	XP_453967.1
Eremothecium gossypii	AGOS_ADR235W	NP_984331.1
Schizosaccharomyces pombe (fission yeast)	adf1	NP_594741.1
Oryza sativa (rice)	Os03g0780400	NP_001051449.1
Xenopus tropicalis (tropical clawed frog)	cfl2	NP_001011156.1
Danio rerio (zebrafish)	cfl2	NP_991263.1
Oryza sativa (rice)	Os12g0628100	NP_001067327.1

Gene Ontology
Bibliography

Related articles in PubMed

Role of the cofilin 2 gene in regulating the myosin heavy chain genes in mouse myoblast C2C12 cells.
Zhu H, Yang H, Zhao S, Zhang J, Liu D, Tian Y, Shen Z, Su Y
International journal of molecular medicine41(2)1096-1102(2018 Feb)

HSPB7 is indispensable for heart development by modulating actin filament assembly.
Wu T, Mu Y, Bogomolovas J, Fang X, Veevers J, Nowak RB, Pappas CT, Gregorio CC, Evans SM, Fowler VM, Chen J
Proceedings of the National Academy of Sciences of the United States of America114(45)11956-11961(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Vessel wall P2Y12 receptor, which promotes VSMCs migration through cofilin dephosphorylation, plays a critical role in the development of atherosclerotic lesions.
Title: P2Y

miR-301a regulates Cofilin-2 in vitro in NRVCM, and in vivo in Cs1-ko mice. Our findings provide an additional and important layer of Cfl2 regulation, which we believe has an extended role in cardiac signal transduction and dilated cardiomyopathy presumably due to the reported involvement of Cfl2 in these mechanisms.
Title: MicroRNA miR-301a is a novel cardiac regulator of Cofilin-2.

deficiency of cofilin-2 is associated with interruption of the cell cycle at several checkpoints, hindering muscle regeneration
Title: Skeletal muscle microRNA and messenger RNA profiling in cofilin-2 deficient mice reveals cell cycle dysregulation hindering muscle regeneration.

Levels of smooth muscle alpha-actin were increased and remained high in developing muscles, suggesting that cofilin2 plays a crucial role during the exchange of alpha-actin isoforms during the early postnatal remodeling of the sarcomere.
Title: Severe protein aggregate myopathy in a knockout mouse model points to an essential role of cofilin2 in sarcomeric actin exchange and muscle maintenance.

cofilin-2, although not critical for muscle development, is essential for muscle maintenance.
Title: Normal myofibrillar development followed by progressive sarcomeric disruption with actin accumulations in a mouse Cfl2 knockout demonstrates requirement of cofilin-2 for muscle maintenance.

The following Cfl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cfl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00610	NM_007688.2 Latest version!	Mus musculus cofilin 2, muscle (Cfl2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu47565	XM_006515452.3 Latest version!	Mus musculus cofilin 2, muscle (Cfl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu47565	XM_006515452.2	Mus musculus cofilin 2, muscle (Cfl2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cfl2 ( NM_007688.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031714.1 Mus musculus cofilin 2, muscle (Cfl2), mRNA.

Price & Availability↑

CloneID	OMu00610
Clone ID Related Accession (Same CDS sequence)	NM_007688.2
Accession Version	NM_007688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Mus musculus(house mouse)
Product	cofilin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC154732.3. On Mar 2, 2007 this sequence version replaced NM_007688.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L29468.1, AK004659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGGCATCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGAAAATCTT CTACACAGGA GGAGATCAAA AAAAGAAAGA AAGCAGTTCT CTTCTGTTTG 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCCAAGC AGATCTTGGT GGGTGACATT 
GGTGACACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGTTGCCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACGTACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCG TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GACGATATTA AGGACCGCTC GACGCTGGGA GAGAAACTGG GGGGCAGTGT TGTAGTTTCC 
CTTGAAGGAA AGCCACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031714.1
CDS	132..632
Translation	MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGSVVVSLEGKPL

Target ORF information:

RefSeq Version	NM_007688.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cofilin 2, muscle (Cfl2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007688.2

ORF Insert Sequence:

ATGGCATCTG GAGTTACAGT GAATGATGAA GTCATCAAAG TTTTTAATGA TATGAAAGTA 
AGAAAATCTT CTACACAGGA GGAGATCAAA AAAAGAAAGA AAGCAGTTCT CTTCTGTTTG 
AGCGATGACA AAAGACAAAT AATTGTAGAG GAAGCCAAGC AGATCTTGGT GGGTGACATT 
GGTGACACTG TAGAGGACCC CTACACATCT TTTGTGAAGT TGTTGCCTCT GAATGATTGC 
CGATATGCTT TGTACGATGC CACGTACGAA ACAAAAGAGT CTAAGAAAGA AGACCTAGTA 
TTTATATTCT GGGCTCCTGA AAGTGCACCG TTAAAAAGCA AGATGATTTA TGCTAGCTCT 
AAAGATGCCA TTAAAAAGAA ATTTACAGGT ATTAAACATG AGTGGCAAGT AAATGGCTTG 
GACGATATTA AGGACCGCTC GACGCTGGGA GAGAAACTGG GGGGCAGTGT TGTAGTTTCC 
CTTGAAGGAA AGCCACTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfl2 ( XM_006515452.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515515.1 Mus musculus cofilin 2, muscle (Cfl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47565
Clone ID Related Accession (Same CDS sequence)	XM_006515452.3 , XM_006515452.2
Accession Version	XM_006515452.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	cofilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006515452.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAA GAAAATCTTC TACACAGGAG GAGATCAAAA AAAGAAAGAA AGCAGTTCTC 
TTCTGTTTGA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCCAAGCA GATCTTGGTG 
GGTGACATTG GTGACACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GTTGCCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACGTACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCGT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ACGATATTAA GGACCGCTCG ACGCTGGGAG AGAAACTGGG GGGCAGTGTT 
GTAGTTTCCC TTGAAGGAAA GCCACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515515.1
CDS	326..775
Translation	MKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGSVVVSLEGKPL

Target ORF information:

RefSeq Version	XM_006515452.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus cofilin 2, muscle (Cfl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515452.2

ORF Insert Sequence:

ATGAAAGTAA GAAAATCTTC TACACAGGAG GAGATCAAAA AAAGAAAGAA AGCAGTTCTC 
TTCTGTTTGA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCCAAGCA GATCTTGGTG 
GGTGACATTG GTGACACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GTTGCCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACGTACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCGT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ACGATATTAA GGACCGCTCG ACGCTGGGAG AGAAACTGGG GGGCAGTGTT 
GTAGTTTCCC TTGAAGGAAA GCCACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfl2 ( XM_006515452.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006515515.1 Mus musculus cofilin 2, muscle (Cfl2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu47565
Clone ID Related Accession (Same CDS sequence)	XM_006515452.3 , XM_006515452.2
Accession Version	XM_006515452.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cofilin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000078.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006515452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTAA GAAAATCTTC TACACAGGAG GAGATCAAAA AAAGAAAGAA AGCAGTTCTC 
TTCTGTTTGA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCCAAGCA GATCTTGGTG 
GGTGACATTG GTGACACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GTTGCCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACGTACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCGT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ACGATATTAA GGACCGCTCG ACGCTGGGAG AGAAACTGGG GGGCAGTGTT 
GTAGTTTCCC TTGAAGGAAA GCCACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006515515.1
CDS	392..841
Translation	MKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGSVVVSLEGKPL

Target ORF information:

RefSeq Version	XM_006515452.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus cofilin 2, muscle (Cfl2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006515452.3

ORF Insert Sequence:

ATGAAAGTAA GAAAATCTTC TACACAGGAG GAGATCAAAA AAAGAAAGAA AGCAGTTCTC 
TTCTGTTTGA GCGATGACAA AAGACAAATA ATTGTAGAGG AAGCCAAGCA GATCTTGGTG 
GGTGACATTG GTGACACTGT AGAGGACCCC TACACATCTT TTGTGAAGTT GTTGCCTCTG 
AATGATTGCC GATATGCTTT GTACGATGCC ACGTACGAAA CAAAAGAGTC TAAGAAAGAA 
GACCTAGTAT TTATATTCTG GGCTCCTGAA AGTGCACCGT TAAAAAGCAA GATGATTTAT 
GCTAGCTCTA AAGATGCCAT TAAAAAGAAA TTTACAGGTA TTAAACATGA GTGGCAAGTA 
AATGGCTTGG ACGATATTAA GGACCGCTCG ACGCTGGGAG AGAAACTGGG GGGCAGTGTT 
GTAGTTTCCC TTGAAGGAAA GCCACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Role of the cofilin 2 gene in regulating the myosin heavy chain genes in mouse myoblast C2C12 cells.
International journal of molecular medicine41(2)1096-1102(2018 Feb)
Zhu H,Yang H,Zhao S,Zhang J,Liu D,Tian Y,Shen Z,Su Y

HSPB7 is indispensable for heart development by modulating actin filament assembly.
Proceedings of the National Academy of Sciences of the United States of America114(45)11956-11961(2017 Nov)
Wu T,Mu Y,Bogomolovas J,Fang X,Veevers J,Nowak RB,Pappas CT,Gregorio CC,Evans SM,Fowler VM,Chen J

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Cfl2 cDNA ORF clone, Mus musculus(house mouse)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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