LOC427201 cDNA ORF clone, Gallus gallus(chicken)

The following LOC427201 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC427201 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa47914 XM_015277658.2
Latest version!
Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa47915 XM_015277659.2
Latest version!
Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa153053 XM_025144889.1
Latest version!
Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa47913 XM_015277657.2
Latest version!
Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa21189 XM_015277655.1
Latest version!
Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OGa21189 XM_025144888.1
Latest version!
Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
OGa47913 XM_015277657.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa47915 XM_015277659.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OGa47914 XM_015277658.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa47914
    Clone ID Related Accession (Same CDS sequence) XM_015277658.2 , XM_015277658.1
    Accession Version XM_015277658.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC
    CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA
    TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT
    ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG
    ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC
    AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT
    ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG
    ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG
    TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA
    CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG
    AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA
    AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC
    AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG
    GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG
    GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC
    GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC
    CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC
    AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC
    TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT
    GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA
    ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA
    GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG
    GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC
    CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC
    CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT
    GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT
    ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA
    CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA
    GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT
    GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA
    GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT
    GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA
    GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA
    CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG
    GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT
    ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA
    GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC
    GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG
    GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT
    GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA
    AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT
    ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG
    GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT
    GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC
    CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC
    CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA
    AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG
    AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG
    CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC
    CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG
    ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA
    GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA
    GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG
    GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC
    ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT
    GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT
    CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG
    GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133144.1
    CDS220..4461
    Translation

    Target ORF information:

    RefSeq Version XM_015277658.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277658.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC
    CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA
    TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT
    ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG
    ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC
    AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT
    ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG
    ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG
    TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA
    CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG
    AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA
    AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC
    AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG
    GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG
    GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC
    GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC
    CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC
    AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC
    TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT
    GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA
    ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA
    GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG
    GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC
    CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC
    CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT
    GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT
    ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA
    CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA
    GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT
    GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA
    GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT
    GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA
    GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA
    CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG
    GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT
    ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA
    GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC
    GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG
    GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT
    GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA
    AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT
    ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG
    GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT
    GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC
    CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC
    CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA
    AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG
    AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG
    CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC
    CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG
    ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA
    GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA
    GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG
    GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC
    ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT
    GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT
    CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG
    GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47914
    Clone ID Related Accession (Same CDS sequence) XM_015277658.2 , XM_015277658.1
    Accession Version XM_015277658.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC
    CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA
    TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT
    ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG
    ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC
    AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT
    ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG
    ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG
    TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA
    CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG
    AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA
    AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC
    AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG
    GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG
    GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC
    GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC
    CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC
    AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC
    TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT
    GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA
    ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA
    GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG
    GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC
    CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC
    CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT
    GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT
    ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA
    CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA
    GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT
    GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA
    GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT
    GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA
    GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA
    CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG
    GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT
    ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA
    GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC
    GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG
    GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT
    GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA
    AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT
    ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG
    GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT
    GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC
    CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC
    CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA
    AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG
    AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG
    CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC
    CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG
    ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA
    GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA
    GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG
    GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC
    ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT
    GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT
    CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG
    GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133144.1
    CDS155..4396
    Translation

    Target ORF information:

    RefSeq Version XM_015277658.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277658.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC
    CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA
    TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT
    ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG
    ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC
    AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT
    ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG
    ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG
    TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA
    CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG
    AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA
    AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC
    AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG
    GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG
    GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC
    GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC
    CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC
    AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC
    TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT
    GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA
    ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA
    GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG
    GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC
    CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC
    CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT
    GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT
    ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA
    CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA
    GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT
    GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA
    GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT
    GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA
    GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA
    CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG
    GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT
    ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA
    GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC
    GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG
    GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT
    GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA
    AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT
    ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG
    GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT
    GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC
    CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC
    CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA
    AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG
    AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG
    CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC
    CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG
    ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA
    GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA
    GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG
    GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC
    ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT
    GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT
    CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG
    GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47915
    Clone ID Related Accession (Same CDS sequence) XM_015277659.1 , XM_015277659.2
    Accession Version XM_015277659.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC
    CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG
    TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA
    CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT
    AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC
    CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG
    GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT
    CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG
    CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC
    CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT
    GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG
    GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT
    CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT
    GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT
    TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA
    TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC
    CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA
    CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT
    ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA
    TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG
    TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC
    GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA
    CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA
    ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA
    CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC
    ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG
    TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG
    CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT
    TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG
    TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA
    GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA
    CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA
    TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC
    TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT
    CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG
    TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA
    GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA
    CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA
    GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT
    GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA
    GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC
    AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT
    AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG
    GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG
    CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG
    AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA
    CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG
    GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT
    CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG
    GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA
    AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC
    ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT
    CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG
    TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC
    CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG
    CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG
    GAGAATATTG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133145.1
    CDS152..4312
    Translation

    Target ORF information:

    RefSeq Version XM_015277659.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277659.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC
    CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG
    TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA
    CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT
    AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC
    CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG
    GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT
    CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG
    CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC
    CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT
    GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG
    GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT
    CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT
    GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT
    TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA
    TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC
    CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA
    CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT
    ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA
    TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG
    TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC
    GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA
    CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA
    ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA
    CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC
    ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG
    TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG
    CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT
    TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG
    TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA
    GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA
    CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA
    TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC
    TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT
    CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG
    TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA
    GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA
    CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA
    GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT
    GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA
    GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC
    AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT
    AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG
    GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG
    CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG
    AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA
    CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG
    GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT
    CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG
    GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA
    AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC
    ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT
    CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG
    TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC
    CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG
    CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG
    GAGAATATTG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47915
    Clone ID Related Accession (Same CDS sequence) XM_015277659.1 , XM_015277659.2
    Accession Version XM_015277659.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4161bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC
    CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG
    TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA
    CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT
    AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC
    CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG
    GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT
    CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG
    CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC
    CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT
    GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG
    GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT
    CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT
    GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT
    TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA
    TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC
    CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA
    CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT
    ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA
    TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG
    TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC
    GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA
    CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA
    ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA
    CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC
    ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG
    TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG
    CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT
    TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG
    TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA
    GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA
    CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA
    TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC
    TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT
    CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG
    TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA
    GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA
    CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA
    GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT
    GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA
    GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC
    AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT
    AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG
    GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG
    CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG
    AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA
    CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG
    GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT
    CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG
    GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA
    AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC
    ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT
    CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG
    TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC
    CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG
    CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG
    GAGAATATTG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133145.1
    CDS219..4379
    Translation

    Target ORF information:

    RefSeq Version XM_015277659.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
    ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG
    CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT
    GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC
    ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA
    GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT
    GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA
    TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA
    CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA
    AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT
    CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG
    ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT
    CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT
    CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC
    CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG
    TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA
    CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT
    AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC
    CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG
    GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT
    CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG
    CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC
    CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT
    GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG
    GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT
    CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT
    GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT
    TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA
    TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC
    CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA
    CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT
    ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA
    TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG
    TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC
    GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA
    CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA
    ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA
    CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC
    ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG
    TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG
    CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT
    TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG
    TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA
    GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA
    CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA
    TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC
    TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT
    CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG
    TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA
    GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA
    CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA
    GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT
    GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA
    GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC
    AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT
    AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG
    GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG
    CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG
    AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA
    CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG
    GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT
    CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG
    GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA
    AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC
    ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT
    CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG
    TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC
    CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG
    CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG
    GAGAATATTG TTTACCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa153053
    Clone ID Related Accession (Same CDS sequence) XM_025144889.1
    Accession Version XM_025144889.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCATTG CTCACGAAGA ATTGCCATCA ACGTGGAGCA TCCTCGGAAG CTGCTATGAA 
    GGAGAGCCTA CACACAAAGA AACTCTTCTC CACCTTGCAA TGAAATTGGG CTTGGTTAAA
    CTTTCAGAGT TCTTAGTGGG CCAGCCTGGA GGGACATTGG CATTAGCGCT ACCTAATGAG
    GATGGAGCAA CACCATTAGA CTTGGCTGAG CAAAACGGGC ACTCCAAACT AGTTGAAGTC
    TTCTCAAATT TCCAGGGCAG CCGCTCGCCT GACGTTGCCA GAGTGGAGAT CAGCAAGGGG
    GCCTGCATGA GCTTTGTGCG CTCGTCAGCA GCTCTGACGC TGACGCTGGA CCACACGGCC
    AAGCACTTAC TGGAGTCAGA TGTGAGGCTC TTCAGGAAGT ACTTCTGGAA CAGGCCGCTG
    CTCCATGAGT GTATCAATTC AAACCAGCTT GAGCCAGTGG AAGATCCAGT AATGCGCTGC
    AGCACAACAA ACTCTGTAGA AGGTCCTGTG AGCTTGGCAC CTGATGACTT GTCCCAGGAT
    GGTGAGAAGC CTGTCCATGG TGCTGTGGTT CCTTCTAAAG AAGTTGAAGA TAATGTCCTG
    CTAGACATAG CACAGAGACA TTCTACCCTT GATATTCTTA AGAAGCTGAA AACTCCACCT
    ACCTTCTTTG CTGCAGCCAG ACTTTCTACT ATGCTGAATG GAAGTGATGA AGTCTATGCG
    AACTGCATGC TAGTAGACCA GGCTGAAGAT TTAAAAATGA ACTACATTCA TGGAGATAGC
    ATTAGCAGTG AAACGTGTCT GAATCCCAGC AGCTCAGCCC CAGTAGCGAA GAGCTCTTCG
    TCTCCTTCCC TTGAGGAGAA TGACCAGTAT AGATGCGATC CAAGTGAAGG AAATCAGTCA
    GTGAAAAATG AAGGAAATAC AGGCTTAAGA CCTACTGTTT CCCCATCTGA TCTGTATGAA
    GCAGATTCCT ACCTTCCATT CAAGACAAAT GAGTTTATTA AGGAACAGGG CCAACTTCAT
    GCTGTTATGA AAAAAAAAAG TTCAAGTCTC GATGACCTTG AAGTAAACAG CAGAAGTGAA
    GGTGTCTTGG GCAGATCCAA TATACCCTAC CCTCTTCTTG AACATCCAGA GGTGAAGGCT
    TGTAGCAGAG ATGGATTAGA TTCTTCTGAG ATGAACATCA GCCGGACAGA ATCACTGCCC
    TTATCTATCA GTCCACAGAC CAAGGAATGT TCACCCTCTG GAATTCGGTC ACGCTCGTAT
    TCCTGTTCCT CACCCAAAGA CTCATTGGGA AGACCTCGCA TTCTTCAGGA CTTCACAGTT
    GGTGATTCAG GTGAAGATGG TGTTCCCATG AACAGTGGCC GATCCCTCCT ACAGGCTCTC
    TCACTCTCTA AGTCAGTGTC CCTGCTCCAC CCCTGTAAAC GAGCATACAG CTTGCCTGAG
    CACTCCCGAG AGAAAAGGAT TCAGGAGGAA GAATGGGATA AGTATATTGT ACCTTCAAAA
    ACAGAGTCTG AAAAATACAA AGTGAGTCGG ACTTTCAGCT TTCTGATGAA TAGAATGACT
    AGCACACGGA ATAAGTACAA GACAAAAAAT AAAGATACCA AAGACAAAGA GAAGCTGAAC
    AGACATAATT TTATAAATGG AACCTTCTCT GGAATTATGC CATGTATGGC CTGTGAAAAG
    GCTCTCCTAG GAAAAGAGTC ACTGCAGTGC TCTTATTGCA ATGTGATTGT GCATAAGAAC
    TGTAAGGAGT GTGTTCCTGT GTGCACTAAG AAATCCCAGG AAAGGTACCA TAGCAAAAAC
    AAACCTCAAG TTGGCATAGG AAATTTATCG TTTGGAGACA TTTCACAGCC TGGGATTTCC
    TCAGTGCATC CTTCGTCCTC TGTACCCATT GTATCTGCTG GGAGGAGGGA AGCCTTGCAG
    CAGTCCCATT CCTTGTCCAA AAGTGTCCCT GGTGCCAGTT TTGAAAGAAG ATCTATGCCG
    TTTCCTGAGC AGGACTCTGA TGCTAGCACA TGGAGGACCA AGTCCAGATC TGAGGAGATG
    TTACAGGCGT TAGGGACATA TTCTTCAATG GACTCTTTTG TGATGGAAGT TGAGGTGGAT
    TCGTCTCAGT GGACTGACAT CAGTTCAGAT GCACACGAGT TTGAGGCGGA GTCATGGAGT
    CTTGTTGTGG ATCCGATGTT CTGTAACAAG CAAGAAAAGG ATGTCGCCAA GAGGCAGGAT
    GTCATTTTTG AGCTGATGCA AACAGAAGTT CATCACATCC ACACCCTGTT CATTATGTCT
    GAAATATTCA GGAAGGGAAT GAAAGAAGAA CTGCAGCTGG ACCACAGCAC GGTGGACAGA
    ATATTCCCCT GCTTAGATGA GCTACTGGAG ATCCATAGGC AGTTCTTTTG TAGAATGAGG
    GAGAGACGGC AGGAATCGTG TGAGGAGGGG AATGAGCGTA ATTTTGTAAT CAGCAGAATC
    GGAGATATCC TTGTGCAACA GTTTTCAGAG GAAAATGCAA CTAAAATGAA GAAAATATAT
    GGAGAATTTT GCAGCCATCA GAAAGAAGCT GTTAATCTCT TCAAAGAGTT GCAGCAAAAC
    AAGAAGTTCC AGAATTTTAT TAAACTGAGG AACAGCAATT TGTTGGCACG CCGCAGAGGA
    ATCCCCGAAT GCATTCTGCT TGTCACTCAG CGAATCACCA AATACCCTGT TCTAGTTGAA
    AGGATACTGC AGTACACAAA AGAAGGAACA GAAGAACATA AAGACCTATG CAAAGCCCTT
    CGTTTAATTA AAGACATGAT TGCAGCAGTA GATTTGAAAG TTAATGAATA CGAGAAAAGG
    CAAAAACTGA TGGAAATTCT CAACCGAACT GAAAACAAAA CATATACAAA ACTGAAAAGT
    GGACACATTT TTAGGAAGCA AGACTTGATG AGGAAAGAGA GGATACTTCT TCATGAAGGT
    TTAGTGTACT GGAAAACAGC TACTGGTCGT TTCAAAGACA CCCTAGCTCT GCTTCTAACT
    GATATGCTGC TGTTCTTGCA AGAAAAAGAT CAGAAGTACG TTTTTGCAGC TGTTGACCAG
    AAGCCTTCAG TAATCTCTCT TCAGAAGCTC ATTGTAAGAG AAGTAGCCAA TGAAGAAAGG
    GGGATGTTTC TGATTAGTGC TTCATCTGCA GGACCTGAGA TGTATGAAGT TCATACGAAC
    TCCAGAGAAG AGCGTAACAG CTGGATGAGG CATATTCAAG ATGCAGTGGA AAGTTGTCCA
    GAGGAACAGG AAGAAGGAAA AATAGGTGAA TTTGATGAGG ACAGACGTAT CGCTGAGGCT
    AAAGCATGCA AAATTCAGAA ATGTCAAGAA TCACTGAGTA ACCAGGACCA GCAAATCTGC
    AGTTGCTTGG AAGACAAATT GCATATCTAT GCAGAACTAG TAGATATGAG TGGATTTGAA
    GATGTTCATG TAGAACCTCA CCTCCTTATC AAACCTGATT ATGGAGAAAC TCCACAGGCT
    TCTTCGTTGC TGGTAGCAGC CCTCAGAGAA GTTGAAAGTC TCCACGTTGC TCTCACGTCA
    TCACAAGTGA ATGAAACGGA CAGATCATCA GAGGAAAGTA CAGAGGAATT AACTTTGAAA
    CACAGACTTA GTGCCAGTGA AATCTTGGAA TCTTTCCCTG GTGATGCAGA AACAAAGGAA
    GTTGAAGAAT CACCTGAAGT TGATCTGAGT GCTTGCTCTA TTGAAAAGGA AATTTCTGAT
    GCCAGTGTAG AGTATAAGGG AGAAAGTACG AGTTTCCCAG GATCTACAGG GACAGAGATT
    GTACAAGCAA TCCAGAATCT AACTCGTCTC TTGTACAGCA TACAGGCAGC GTTAGCTATC
    CAGGACAGCC ACAGAGAGCT CCATAGGTTA CTCCTGCAAG AGAATGAGAA GACCAGTCGT
    GGCCATAGTT CTCGGATACC CCTCTTCCTG GAACCAGAAA AATACCGTAA TTCAGAAAAA
    CAAAGGGAGG AGCTGGCAAA CATCAACAAA CTCCAGCAGC AGTTCCAGCA AGAGCAGCAG
    CGCTGGCTGC GTGAATGCGA GCAGCGGCAG CAAGAGCTGG AGGTAAGGGC AGGCCTGCTG
    GAGCAGAGGG AGCAGGCCTG CAGCTGCCAG GAGGAGCTGC TGGAGAAGAG CCGCCAGGGG
    CTTGCACTGC AGTCGCAGGA GTTCCAGCAG AGCCTGGAGA GGCTGCGGGA AGGGCAGAAG
    TTGTTGGAGA AAAAAAGGGA CAGTATCAAA ATGCAGAAGA AGCTCCTCTG GCACTGGAAG
    CATGGTCTCC AAAGCAGCCT GCTGCTGCCC ACAGGGAGCC ACGAGATAAT GGGACACGAT
    CAGTTGGATA GCTTACATGG AGAAAATGCG TTCTATTTAA ATGAAGCTGT AGTACGCGTG
    TCTCTAAGCG GCCTCAACAG ATCAGATTCT TCTCTTGTTT ATGAAGATGG TGTGTATGGG
    CTGGGCATTT CAGACTTGGA TATGGCAAGG ACTTGTGAAA ATCAGGTGAA CCTCAGGGTG
    GATACTCCTT GCCAACCAGC ATTGACCAAT GAACTGTGGA AATCCACTGG CCCTTACCAG
    CAGATGTCTT TCTCCTGCAA AAGCAACAAG GATGCTTTTA ATAATGATCT AAATGCATCA
    CAGACCCAGG GTCCCCAGGC AGGCAGTCCA AACCATGGAT CCATTCAGCC ACCACAAGTG
    GAACCGCTGA TGCTGAACCA CCAGCCTGAG AGCCTGCAGG ATGCGGAAAG TGGAGACGGA
    GGGGAGGAGA ATATTGTTTA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000657.1
    CDS228..4994
    Translation

    Target ORF information:

    RefSeq Version XM_025144889.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144889.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCCATTG CTCACGAAGA ATTGCCATCA ACGTGGAGCA TCCTCGGAAG CTGCTATGAA 
    GGAGAGCCTA CACACAAAGA AACTCTTCTC CACCTTGCAA TGAAATTGGG CTTGGTTAAA
    CTTTCAGAGT TCTTAGTGGG CCAGCCTGGA GGGACATTGG CATTAGCGCT ACCTAATGAG
    GATGGAGCAA CACCATTAGA CTTGGCTGAG CAAAACGGGC ACTCCAAACT AGTTGAAGTC
    TTCTCAAATT TCCAGGGCAG CCGCTCGCCT GACGTTGCCA GAGTGGAGAT CAGCAAGGGG
    GCCTGCATGA GCTTTGTGCG CTCGTCAGCA GCTCTGACGC TGACGCTGGA CCACACGGCC
    AAGCACTTAC TGGAGTCAGA TGTGAGGCTC TTCAGGAAGT ACTTCTGGAA CAGGCCGCTG
    CTCCATGAGT GTATCAATTC AAACCAGCTT GAGCCAGTGG AAGATCCAGT AATGCGCTGC
    AGCACAACAA ACTCTGTAGA AGGTCCTGTG AGCTTGGCAC CTGATGACTT GTCCCAGGAT
    GGTGAGAAGC CTGTCCATGG TGCTGTGGTT CCTTCTAAAG AAGTTGAAGA TAATGTCCTG
    CTAGACATAG CACAGAGACA TTCTACCCTT GATATTCTTA AGAAGCTGAA AACTCCACCT
    ACCTTCTTTG CTGCAGCCAG ACTTTCTACT ATGCTGAATG GAAGTGATGA AGTCTATGCG
    AACTGCATGC TAGTAGACCA GGCTGAAGAT TTAAAAATGA ACTACATTCA TGGAGATAGC
    ATTAGCAGTG AAACGTGTCT GAATCCCAGC AGCTCAGCCC CAGTAGCGAA GAGCTCTTCG
    TCTCCTTCCC TTGAGGAGAA TGACCAGTAT AGATGCGATC CAAGTGAAGG AAATCAGTCA
    GTGAAAAATG AAGGAAATAC AGGCTTAAGA CCTACTGTTT CCCCATCTGA TCTGTATGAA
    GCAGATTCCT ACCTTCCATT CAAGACAAAT GAGTTTATTA AGGAACAGGG CCAACTTCAT
    GCTGTTATGA AAAAAAAAAG TTCAAGTCTC GATGACCTTG AAGTAAACAG CAGAAGTGAA
    GGTGTCTTGG GCAGATCCAA TATACCCTAC CCTCTTCTTG AACATCCAGA GGTGAAGGCT
    TGTAGCAGAG ATGGATTAGA TTCTTCTGAG ATGAACATCA GCCGGACAGA ATCACTGCCC
    TTATCTATCA GTCCACAGAC CAAGGAATGT TCACCCTCTG GAATTCGGTC ACGCTCGTAT
    TCCTGTTCCT CACCCAAAGA CTCATTGGGA AGACCTCGCA TTCTTCAGGA CTTCACAGTT
    GGTGATTCAG GTGAAGATGG TGTTCCCATG AACAGTGGCC GATCCCTCCT ACAGGCTCTC
    TCACTCTCTA AGTCAGTGTC CCTGCTCCAC CCCTGTAAAC GAGCATACAG CTTGCCTGAG
    CACTCCCGAG AGAAAAGGAT TCAGGAGGAA GAATGGGATA AGTATATTGT ACCTTCAAAA
    ACAGAGTCTG AAAAATACAA AGTGAGTCGG ACTTTCAGCT TTCTGATGAA TAGAATGACT
    AGCACACGGA ATAAGTACAA GACAAAAAAT AAAGATACCA AAGACAAAGA GAAGCTGAAC
    AGACATAATT TTATAAATGG AACCTTCTCT GGAATTATGC CATGTATGGC CTGTGAAAAG
    GCTCTCCTAG GAAAAGAGTC ACTGCAGTGC TCTTATTGCA ATGTGATTGT GCATAAGAAC
    TGTAAGGAGT GTGTTCCTGT GTGCACTAAG AAATCCCAGG AAAGGTACCA TAGCAAAAAC
    AAACCTCAAG TTGGCATAGG AAATTTATCG TTTGGAGACA TTTCACAGCC TGGGATTTCC
    TCAGTGCATC CTTCGTCCTC TGTACCCATT GTATCTGCTG GGAGGAGGGA AGCCTTGCAG
    CAGTCCCATT CCTTGTCCAA AAGTGTCCCT GGTGCCAGTT TTGAAAGAAG ATCTATGCCG
    TTTCCTGAGC AGGACTCTGA TGCTAGCACA TGGAGGACCA AGTCCAGATC TGAGGAGATG
    TTACAGGCGT TAGGGACATA TTCTTCAATG GACTCTTTTG TGATGGAAGT TGAGGTGGAT
    TCGTCTCAGT GGACTGACAT CAGTTCAGAT GCACACGAGT TTGAGGCGGA GTCATGGAGT
    CTTGTTGTGG ATCCGATGTT CTGTAACAAG CAAGAAAAGG ATGTCGCCAA GAGGCAGGAT
    GTCATTTTTG AGCTGATGCA AACAGAAGTT CATCACATCC ACACCCTGTT CATTATGTCT
    GAAATATTCA GGAAGGGAAT GAAAGAAGAA CTGCAGCTGG ACCACAGCAC GGTGGACAGA
    ATATTCCCCT GCTTAGATGA GCTACTGGAG ATCCATAGGC AGTTCTTTTG TAGAATGAGG
    GAGAGACGGC AGGAATCGTG TGAGGAGGGG AATGAGCGTA ATTTTGTAAT CAGCAGAATC
    GGAGATATCC TTGTGCAACA GTTTTCAGAG GAAAATGCAA CTAAAATGAA GAAAATATAT
    GGAGAATTTT GCAGCCATCA GAAAGAAGCT GTTAATCTCT TCAAAGAGTT GCAGCAAAAC
    AAGAAGTTCC AGAATTTTAT TAAACTGAGG AACAGCAATT TGTTGGCACG CCGCAGAGGA
    ATCCCCGAAT GCATTCTGCT TGTCACTCAG CGAATCACCA AATACCCTGT TCTAGTTGAA
    AGGATACTGC AGTACACAAA AGAAGGAACA GAAGAACATA AAGACCTATG CAAAGCCCTT
    CGTTTAATTA AAGACATGAT TGCAGCAGTA GATTTGAAAG TTAATGAATA CGAGAAAAGG
    CAAAAACTGA TGGAAATTCT CAACCGAACT GAAAACAAAA CATATACAAA ACTGAAAAGT
    GGACACATTT TTAGGAAGCA AGACTTGATG AGGAAAGAGA GGATACTTCT TCATGAAGGT
    TTAGTGTACT GGAAAACAGC TACTGGTCGT TTCAAAGACA CCCTAGCTCT GCTTCTAACT
    GATATGCTGC TGTTCTTGCA AGAAAAAGAT CAGAAGTACG TTTTTGCAGC TGTTGACCAG
    AAGCCTTCAG TAATCTCTCT TCAGAAGCTC ATTGTAAGAG AAGTAGCCAA TGAAGAAAGG
    GGGATGTTTC TGATTAGTGC TTCATCTGCA GGACCTGAGA TGTATGAAGT TCATACGAAC
    TCCAGAGAAG AGCGTAACAG CTGGATGAGG CATATTCAAG ATGCAGTGGA AAGTTGTCCA
    GAGGAACAGG AAGAAGGAAA AATAGGTGAA TTTGATGAGG ACAGACGTAT CGCTGAGGCT
    AAAGCATGCA AAATTCAGAA ATGTCAAGAA TCACTGAGTA ACCAGGACCA GCAAATCTGC
    AGTTGCTTGG AAGACAAATT GCATATCTAT GCAGAACTAG TAGATATGAG TGGATTTGAA
    GATGTTCATG TAGAACCTCA CCTCCTTATC AAACCTGATT ATGGAGAAAC TCCACAGGCT
    TCTTCGTTGC TGGTAGCAGC CCTCAGAGAA GTTGAAAGTC TCCACGTTGC TCTCACGTCA
    TCACAAGTGA ATGAAACGGA CAGATCATCA GAGGAAAGTA CAGAGGAATT AACTTTGAAA
    CACAGACTTA GTGCCAGTGA AATCTTGGAA TCTTTCCCTG GTGATGCAGA AACAAAGGAA
    GTTGAAGAAT CACCTGAAGT TGATCTGAGT GCTTGCTCTA TTGAAAAGGA AATTTCTGAT
    GCCAGTGTAG AGTATAAGGG AGAAAGTACG AGTTTCCCAG GATCTACAGG GACAGAGATT
    GTACAAGCAA TCCAGAATCT AACTCGTCTC TTGTACAGCA TACAGGCAGC GTTAGCTATC
    CAGGACAGCC ACAGAGAGCT CCATAGGTTA CTCCTGCAAG AGAATGAGAA GACCAGTCGT
    GGCCATAGTT CTCGGATACC CCTCTTCCTG GAACCAGAAA AATACCGTAA TTCAGAAAAA
    CAAAGGGAGG AGCTGGCAAA CATCAACAAA CTCCAGCAGC AGTTCCAGCA AGAGCAGCAG
    CGCTGGCTGC GTGAATGCGA GCAGCGGCAG CAAGAGCTGG AGGTAAGGGC AGGCCTGCTG
    GAGCAGAGGG AGCAGGCCTG CAGCTGCCAG GAGGAGCTGC TGGAGAAGAG CCGCCAGGGG
    CTTGCACTGC AGTCGCAGGA GTTCCAGCAG AGCCTGGAGA GGCTGCGGGA AGGGCAGAAG
    TTGTTGGAGA AAAAAAGGGA CAGTATCAAA ATGCAGAAGA AGCTCCTCTG GCACTGGAAG
    CATGGTCTCC AAAGCAGCCT GCTGCTGCCC ACAGGGAGCC ACGAGATAAT GGGACACGAT
    CAGTTGGATA GCTTACATGG AGAAAATGCG TTCTATTTAA ATGAAGCTGT AGTACGCGTG
    TCTCTAAGCG GCCTCAACAG ATCAGATTCT TCTCTTGTTT ATGAAGATGG TGTGTATGGG
    CTGGGCATTT CAGACTTGGA TATGGCAAGG ACTTGTGAAA ATCAGGTGAA CCTCAGGGTG
    GATACTCCTT GCCAACCAGC ATTGACCAAT GAACTGTGGA AATCCACTGG CCCTTACCAG
    CAGATGTCTT TCTCCTGCAA AAGCAACAAG GATGCTTTTA ATAATGATCT AAATGCATCA
    CAGACCCAGG GTCCCCAGGC AGGCAGTCCA AACCATGGAT CCATTCAGCC ACCACAAGTG
    GAACCGCTGA TGCTGAACCA CCAGCCTGAG AGCCTGCAGG ATGCGGAAAG TGGAGACGGA
    GGGGAGGAGA ATATTGTTTA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47913
    Clone ID Related Accession (Same CDS sequence) XM_015277657.2 , XM_015277657.1
    Accession Version XM_015277657.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
    AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG
    CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA
    GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT
    TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA
    GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT
    GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA
    CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC
    AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC
    CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT
    CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG
    AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT
    ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA
    TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA
    AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC
    AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA
    GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG
    ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA
    GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT
    GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG
    TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG
    ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC
    AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC
    AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT
    GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG
    TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT
    GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA
    GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC
    TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC
    AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT
    GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA
    TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC
    ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG
    TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG
    CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA
    ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT
    GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG
    TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA
    CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT
    CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT
    ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG
    CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA
    AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG
    ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT
    CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG
    CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA
    GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG
    CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT
    CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT
    GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC
    AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA
    AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG
    AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA
    TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT
    CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA
    GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG
    GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT
    GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA
    GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG
    GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT
    CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG
    CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA
    CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA
    AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC
    GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC
    TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG
    GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG
    GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC
    CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT
    GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC
    AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG
    GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA
    GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC
    AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG
    GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC
    CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT
    TACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133143.1
    CDS1568..6136
    Translation

    Target ORF information:

    RefSeq Version XM_015277657.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277657.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
    AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG
    CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA
    GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT
    TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA
    GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT
    GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA
    CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC
    AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC
    CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT
    CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG
    AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT
    ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA
    TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA
    AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC
    AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA
    GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG
    ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA
    GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT
    GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG
    TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG
    ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC
    AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC
    AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT
    GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG
    TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT
    GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA
    GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC
    TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC
    AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT
    GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA
    TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC
    ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG
    TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG
    CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA
    ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT
    GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG
    TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA
    CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT
    CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT
    ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG
    CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA
    AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG
    ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT
    CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG
    CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA
    GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG
    CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT
    CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT
    GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC
    AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA
    AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG
    AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA
    TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT
    CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA
    GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG
    GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT
    GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA
    GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG
    GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT
    CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG
    CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA
    CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA
    AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC
    GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC
    TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG
    GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG
    GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC
    CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT
    GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC
    AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG
    GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA
    GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC
    AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG
    GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC
    CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT
    TACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa47913
    Clone ID Related Accession (Same CDS sequence) XM_015277657.2 , XM_015277657.1
    Accession Version XM_015277657.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
    AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG
    CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA
    GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT
    TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA
    GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT
    GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA
    CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC
    AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC
    CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT
    CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG
    AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT
    ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA
    TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA
    AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC
    AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA
    GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG
    ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA
    GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT
    GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG
    TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG
    ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC
    AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC
    AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT
    GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG
    TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT
    GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA
    GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC
    TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC
    AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT
    GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA
    TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC
    ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG
    TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG
    CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA
    ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT
    GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG
    TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA
    CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT
    CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT
    ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG
    CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA
    AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG
    ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT
    CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG
    CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA
    GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG
    CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT
    CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT
    GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC
    AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA
    AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG
    AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA
    TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT
    CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA
    GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG
    GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT
    GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA
    GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG
    GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT
    CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG
    CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA
    CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA
    AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC
    GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC
    TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG
    GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG
    GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC
    CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT
    GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC
    AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG
    GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA
    GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC
    AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG
    GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC
    CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT
    TACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133143.1
    CDS36..4604
    Translation

    Target ORF information:

    RefSeq Version XM_015277657.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277657.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
    AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG
    CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA
    GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT
    TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA
    GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT
    GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA
    CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC
    AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC
    CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT
    CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG
    AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT
    ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA
    TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA
    AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC
    AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA
    GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG
    ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA
    GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT
    GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG
    TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG
    ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC
    AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC
    AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT
    GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG
    TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT
    GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA
    GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC
    TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC
    AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT
    GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA
    TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC
    ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG
    TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG
    CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA
    ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT
    GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG
    TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA
    CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT
    CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT
    ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG
    CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA
    AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG
    ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT
    CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG
    CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA
    GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG
    CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT
    CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT
    GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC
    AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA
    AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG
    AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA
    TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT
    CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA
    GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG
    GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT
    GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA
    GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG
    GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT
    CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG
    CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA
    CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA
    AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC
    GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC
    TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG
    GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG
    GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC
    CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT
    GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC
    AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG
    GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA
    GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC
    AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG
    GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC
    CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT
    TACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa21189
    Clone ID Related Accession (Same CDS sequence) XM_025144888.1 , XM_015277655.1
    Accession Version XM_025144888.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
    GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG
    AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA
    GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA
    CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT
    CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA
    AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT
    GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT
    ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG
    TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA
    ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT
    TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG
    AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA
    CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG
    TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA
    AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG
    CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA
    GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT
    GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG
    CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT
    GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC
    CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT
    GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC
    TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG
    AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG
    GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA
    GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC
    AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC
    TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA
    GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT
    AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA
    GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT
    GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG
    AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT
    TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA
    GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG
    TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA
    GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT
    CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT
    TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG
    CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG
    TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG
    TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG
    GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT
    GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC
    AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC
    TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG
    CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC
    CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT
    TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC
    CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA
    TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG
    CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT
    AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG
    ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT
    TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC
    TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG
    CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA
    GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT
    CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA
    GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG
    GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC
    AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG
    GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT
    GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG
    CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG
    AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT
    AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA
    TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA
    GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA
    ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC
    CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT
    TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG
    GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG
    CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG
    GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG
    CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG
    AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC
    CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT
    AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC
    GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT
    TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT
    TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT
    TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG
    GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG
    ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG
    AATATTGTTT ACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000656.1
    CDS153..5330
    Translation

    Target ORF information:

    RefSeq Version XM_025144888.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144888.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
    GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG
    AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA
    GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA
    CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT
    CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA
    AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT
    GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT
    ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG
    TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA
    ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT
    TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG
    AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA
    CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG
    TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA
    AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG
    CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA
    GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT
    GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG
    CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT
    GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC
    CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT
    GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC
    TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG
    AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG
    GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA
    GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC
    AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC
    TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA
    GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT
    AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA
    GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT
    GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG
    AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT
    TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA
    GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG
    TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA
    GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT
    CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT
    TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG
    CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG
    TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG
    TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG
    GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT
    GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC
    AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC
    TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG
    CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC
    CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT
    TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC
    CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA
    TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG
    CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT
    AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG
    ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT
    TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC
    TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG
    CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA
    GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT
    CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA
    GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG
    GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC
    AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG
    GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT
    GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG
    CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG
    AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT
    AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA
    TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA
    GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA
    ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC
    CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT
    TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG
    GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG
    CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG
    GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG
    CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG
    AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC
    CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT
    AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC
    GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT
    TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT
    TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT
    TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG
    GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG
    ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG
    AATATTGTTT ACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa21189
    Clone ID Related Accession (Same CDS sequence) XM_025144888.1 , XM_015277655.1
    Accession Version XM_015277655.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product rho guanine nucleotide exchange factor 28 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
    GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG
    AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA
    GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA
    CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT
    CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA
    AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT
    GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT
    ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG
    TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA
    ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT
    TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG
    AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA
    CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG
    TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA
    AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG
    CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA
    GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT
    GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG
    CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT
    GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC
    CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT
    GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC
    TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG
    AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG
    GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA
    GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC
    AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC
    TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA
    GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT
    AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA
    GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT
    GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG
    AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT
    TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA
    GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG
    TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA
    GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT
    CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT
    TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG
    CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG
    TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG
    TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG
    GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT
    GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC
    AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC
    TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG
    CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC
    CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT
    TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC
    CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA
    TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG
    CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT
    AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG
    ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT
    TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC
    TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG
    CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA
    GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT
    CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA
    GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG
    GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC
    AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG
    GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT
    GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG
    CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG
    AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT
    AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA
    TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA
    GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA
    ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC
    CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT
    TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG
    GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG
    CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG
    GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG
    CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG
    AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC
    CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT
    AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC
    GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT
    TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT
    TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT
    TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG
    GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG
    ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG
    AATATTGTTT ACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015133141.1
    CDS364..5541
    Translation

    Target ORF information:

    RefSeq Version XM_015277655.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015277655.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
    GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG
    AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA
    GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA
    CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT
    CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA
    AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT
    GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT
    ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG
    TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA
    ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT
    TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG
    AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA
    CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG
    TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA
    AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG
    CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA
    GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT
    GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG
    CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT
    GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC
    CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT
    GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC
    TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG
    AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG
    GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA
    GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC
    AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC
    TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA
    GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT
    AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA
    GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT
    GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG
    AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT
    TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA
    GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG
    TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA
    GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT
    CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT
    TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG
    CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG
    TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG
    TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG
    GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT
    GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC
    AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC
    TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG
    CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC
    CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT
    TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC
    CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA
    TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG
    CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT
    AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG
    ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT
    TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC
    TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG
    CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA
    GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT
    CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA
    GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG
    GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC
    AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG
    GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT
    GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG
    CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG
    AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT
    AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA
    TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA
    GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA
    ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC
    CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT
    TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG
    GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG
    CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG
    GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG
    CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG
    AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC
    CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT
    AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC
    GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT
    TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT
    TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT
    TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG
    GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG
    ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG
    AATATTGTTT ACCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.