ARHGEF28 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_005248568.4	XP_005248625.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_011543546.1	XP_011541848.1	rho guanine nucleotide exchange factor 28 isoform X1
NM_001244364.2	NP_001231293.1	rho guanine nucleotide exchange factor 28 isoform 3
NM_001080479.2	NP_001073948.2	rho guanine nucleotide exchange factor 28 isoform 1
NM_001244364.1	NP_001231293.1	rho guanine nucleotide exchange factor 28 isoform 3
XM_017009703.1	XP_016865192.1	rho guanine nucleotide exchange factor 28 isoform X5
NM_001177693.1	NP_001171164.1	rho guanine nucleotide exchange factor 28 isoform 2
XM_011543546.2	XP_011541848.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_011543547.2	XP_011541849.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_017009702.1	XP_016865191.1	rho guanine nucleotide exchange factor 28 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu95987	XM_017009703.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu95986	XM_017009702.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu00087	NM_001177693.1 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu11947	NM_001244364.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21533	NM_001080479.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu21533	XM_011543546.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu39668	XM_005248568.4 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu71320	XM_011543547.2 Latest version!	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu11947	NM_001244364.1	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu21533	XM_011543546.1	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

ARHGEF28 ( XM_017009703.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865192.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu95987
Clone ID Related Accession (Same CDS sequence)	XM_017009703.1
Accession Version	XM_017009703.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACCAT CAAGTTCGTG TGCTTCCAAC TTGAATCTTT CTTTTGGTTG GCATGGATTT 
GAAAAGGAAC AAAGTCATCT AAAGAAAAGA AGTTCTAGCC TTGATGCCTT GGACGCCGAC 
AGTGAAGGGG AAGGGCATTC TGAGCCATCC CACATCTGTT ACACTCCAGG GTCTCAGAGC 
TCCTCAAGAA CTGGGATTCC TAGTGGGGAT GAATTGGACT CTTTTGAGAC TAACACTGAA 
CCGGATTTTA ATATCTCCAG GGCTGAATCC CTTCCTCTAT CAAGTAATCT ACAGTCGAAG 
GAATCACTGC TTTCTGGAGT TCGCTCACGT TCTTATTCTT GCTCATCACC CAAAATTTCT 
TTAGGAAAAA CTCGTTTGGT GCGTGAATTA ACAGTATGCA GTTCAAGTGA AGAGCAAAGA 
GCTTACAGCT TATCGGAGCC ACCAAGAGAA AACAGAATTC AGGAAGAAGA ATGGGATAAA 
TACATCATAC CTGCCAAATC AGAGTCTGAA AAATATAAAG TGAGTCGAAC TTTCAGTTTC 
CTCATGAATA GGATGACTAG CCCTCGGAAT AAATCAAAGA CAAAAAGCAA GGATGCCAAA 
GATAAAGAGA AGCTGAATCG ACATCAGTTT GCCCCAGGAA CATTCTCTGG GGTTCTGCAG 
TGTTTGGTTT GTGATAAAAC ACTCCTGGGG AAAGAGTCAC TGCAGTGTTC TAACTGTAAT 
GCAAATGTGC ACAAAGGTTG TAAAGATGCT GCGCCTGCAT GCACCAAGAA ATTCCAAGAG 
AAATATAACA AGAACAAACC ACAGACCATC CTTGGAAATT CTTCATTTAG AGACATCCCA 
CAGCCTGGTC TCTCCTTGCA CCCTTCTTCC TCCGTGCCTG TTGGATTGCC GACTGGAAGG 
AGGGAGACTG TGGGACAGGT CCATCCATTG TCCAGAAGTG TTCCAGGCAC CACCTTGGAA 
AGCTTCAGGA GGTCAGCCAC ATCCTTGGAG TCTGAGAGTG ACCATAACAG CTGCAGAAGC 
AGGTCTCATT CTGATGAGCT GCTACAGTCC ATGGGCTCTT CTCCCTCTAC AGAGTCTTTC 
ATAATGGAAG ATGTTGTGGA TTCTTCTCTG TGGAGTGACC TCAGCAGTGA TGCCCAGGAG 
TTTGAAGCAG AATCTTGGAG TCTTGTGGTG GATCCCTCAT TTTGTAATAG GCAGGAGAAG 
GATGTCATCA AAAGACAGGA TGTCATTTTT GAGCTAATGC AAACAGAGAT GCATCACATC 
CAGACCCTGT TCATCATGTC TGAGATCTTC AGGAAAGGCA TGAAAGAGGA GCTGCAGCTG 
GACCACAGCA CCGTGGATAA AATTTTCCCC TGTTTAGATG AGTTGCTTGA AATCCACAGG 
CATTTCTTCT ACAGTATGAA GGAACGAAGG CAGGAATCCT GTGCTGGCAG CGACAGGAAT 
TTTGTGATCG ACCGAATTGG AGATATTTTG GTACAACAGT TTTCAGAAGA AAATGCAAGT 
AAAATGAAGA AAATATATGG AGAATTCTGT TGCCATCATA AAGAAGCTGT TAACCTCTTT 
AAAGAACTCC AGCAGAATAA AAAGTTTCAG AATTTTATTA AGCTCCGAAA TAGTAATCTT 
TTGGCTCGAC GCCGAGGAAT TCCAGAATGC ATTCTGTTGG TCACTCAGCG TATTACAAAA 
TACCCTGTCT TGGTGGAAAG GATATTGCAG TACACAAAGG AAAGAACTGA GGAACATAAA 
GACTTACGCA AAGCGCTTTG CTTAATTAAA GACATGATTG CAACAGTGGA TTTAAAAGTC 
AATGAATATG AGAAAAACCA AAAATGGCTT GAGATCCTAA ATAAGATTGA AAACAAAACA 
TACACGAAGC TCAAAAATGG ACATGTGTTT AGGAAGCAGG CACTGATGAG TGAAGAAAGG 
ACTCTGTTAT ATGATGGCCT TGTTTACTGG AAAACTGCTA CAGGTCGTTT CAAAGATATC 
CTAGCTCTAC TTCTAACTGA TGTGCTGCTC TTTTTACAAG AAAAAGACCA GAAATACATC 
TTTGCAGCCG TTGATCAGAA GCCATCAGTT ATTTCCCTTC AAAAGCTTAT TGCTAGAGAA 
GTTGCTAATG AGGAGAGAGG AATGTTTCTG ATCAGTGCTT CATCTGCTGG TCCTGAGATG 
TATGAAATTC ACACCAATTC CAAGGAGGAA CGCAATAACT GGATGAGACG GATCCAGCAG 
GCTGTAGAAA GTTGTCCTGA AGAAAAAGGG GGAAGGACAA GTGAATCTGA TGAAGACAAG 
AGGAAAGCTG AAGCCAGAGT GGCCAAAATT CAGCAATGTC AAGAAATACT CACTAACCAA 
GACCAACAAA TTTGTGCGTA TTTGGAGGAG AAGCTGCATA TCTATGCTGA ACTTGGAGAA 
CTGAGCGGAT TTGAGGACGT CCATCTAGAG CCCCACCTCC TTATTAAACC TGACCCAGGC 
GAGCCTCCCC AGGCAGCCTC ATTACTGGCA GCAGCACTGA AAGAAGCTGA GAGCCTACAA 
GTTGCAGTGA AGGCCTCACA GATGGGCGCC GTGAGTCAAT CATGTGAGGA CAGTTGTGGA 
GACTCTGTCT TGGCGGACAC ACTCAGTTCT CATGATGTAC CAGGATCACC GACTGCCTCA 
TTAGTCACAG GAGGGAGAGA AGGAAGAGGC TGTTCGGATG TGGATCCCGG GATCCAGGGT 
GTGGTAACCG ACTTGGCCGT CTCTGATGCA GGGGAGAAGG TGGAATGTAG AAATTTTCCA 
GGTTCTTCAC AATCAGAGAT TATACAAGCC ATACAGAATT TAACCCGTCT CTTATACAGC 
CTTCAGGCCG CCTTGACCAT TCAGGACAGC CACATTGAGA TCCACAGGCT GGTTCTCCAG 
CAGCAGGAGG GCCTGTCTCT CGGCCACTCT ATCCTCCGAG GCGGCCCCTT GCAGGACCAG 
AAGTCTCGCG ACGCGGACAG GCAGCATGAG GAGCTGGCCA ATGTGCACCA GCTTCAGCAC 
CAGCTCCAGC AGGAGCAGCG GCGCTGGCTG CGCAGGTGTG AGCAGCAGCA GCGGGCGCAG 
GCGACCAGGG AGAGCTGGCT GCAGGAGCGG GAGCGGGAGT GCCAGTCGCA GGAGGAGCTG 
CTGCTGCGGA GCCGGGGCGA GCTGGACCTC CAGCTCCAGG AGTACCAGCA CAGCCTGGAG 
CGGCTGAGGG AGGGCCAGCG CCTGGTGGAG AGGGAGCAGG CGAGGATGCG GGCCCAGCAG 
AGCCTGCTGG GCCACTGGAA GCACGGCCGG CAGAGGAGCC TGCCCGCGGT GCTCCTTCCG 
GGTGGCCCCG AGGTAATGGA ACTTAATCGA TCTGAGAGTT TATGTCATGA AAACTCATTC 
TTCATCAATG AAGCTTTAGT ACAAATGTCA TTTAACACTT TCAACAAACT GAATCCATCA 
GTTATCCATC AGGATGCCAC TTACCCTACA ACTCAATCTC ATTCTGACTT GGTGAGGACT 
AGTGAACATC AAGTAGACCT CAAGGTGGAC CCTTCTCAGC CTTCGAATGT CAGTCACAAA 
CTGTGGACAG CCGCTGGTTC CGGCCATCAG ATACTTCCTT TCCATGAAAG CAGCAAGGAT 
TCTTGTAAAA ATGGCTCCAG TATGACAAAG TGCAGTTGTA CGTTGACATC TCCCCCGGGA 
CTGTGGACTG GAACCACATC TACTTTGAAA GATTTGGACA CCTCCCACAC TGAGTCCCCA 
ACCCCCCATG ACTCAAATTC ACACCGCCCT CAACTGCAGG CGTTTATAAC AGAAGCAAAG 
CTAAATCTAC CGACAAGGAC AATGACCAGA CAAGATGGGG AAACTGGAGA TGGAGCCAAA 
GAAAATATTG TTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865192.1
CDS	250..4110
Translation	MSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	XM_017009703.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009703.1

ORF Insert Sequence:

ATGTCACCAT CAAGTTCGTG TGCTTCCAAC TTGAATCTTT CTTTTGGTTG GCATGGATTT 
GAAAAGGAAC AAAGTCATCT AAAGAAAAGA AGTTCTAGCC TTGATGCCTT GGACGCCGAC 
AGTGAAGGGG AAGGGCATTC TGAGCCATCC CACATCTGTT ACACTCCAGG GTCTCAGAGC 
TCCTCAAGAA CTGGGATTCC TAGTGGGGAT GAATTGGACT CTTTTGAGAC TAACACTGAA 
CCGGATTTTA ATATCTCCAG GGCTGAATCC CTTCCTCTAT CAAGTAATCT ACAGTCGAAG 
GAATCACTGC TTTCTGGAGT TCGCTCACGT TCTTATTCTT GCTCATCACC CAAAATTTCT 
TTAGGAAAAA CTCGTTTGGT GCGTGAATTA ACAGTATGCA GTTCAAGTGA AGAGCAAAGA 
GCTTACAGCT TATCGGAGCC ACCAAGAGAA AACAGAATTC AGGAAGAAGA ATGGGATAAA 
TACATCATAC CTGCCAAATC AGAGTCTGAA AAATATAAAG TGAGTCGAAC TTTCAGTTTC 
CTCATGAATA GGATGACTAG CCCTCGGAAT AAATCAAAGA CAAAAAGCAA GGATGCCAAA 
GATAAAGAGA AGCTGAATCG ACATCAGTTT GCCCCAGGAA CATTCTCTGG GGTTCTGCAG 
TGTTTGGTTT GTGATAAAAC ACTCCTGGGG AAAGAGTCAC TGCAGTGTTC TAACTGTAAT 
GCAAATGTGC ACAAAGGTTG TAAAGATGCT GCGCCTGCAT GCACCAAGAA ATTCCAAGAG 
AAATATAACA AGAACAAACC ACAGACCATC CTTGGAAATT CTTCATTTAG AGACATCCCA 
CAGCCTGGTC TCTCCTTGCA CCCTTCTTCC TCCGTGCCTG TTGGATTGCC GACTGGAAGG 
AGGGAGACTG TGGGACAGGT CCATCCATTG TCCAGAAGTG TTCCAGGCAC CACCTTGGAA 
AGCTTCAGGA GGTCAGCCAC ATCCTTGGAG TCTGAGAGTG ACCATAACAG CTGCAGAAGC 
AGGTCTCATT CTGATGAGCT GCTACAGTCC ATGGGCTCTT CTCCCTCTAC AGAGTCTTTC 
ATAATGGAAG ATGTTGTGGA TTCTTCTCTG TGGAGTGACC TCAGCAGTGA TGCCCAGGAG 
TTTGAAGCAG AATCTTGGAG TCTTGTGGTG GATCCCTCAT TTTGTAATAG GCAGGAGAAG 
GATGTCATCA AAAGACAGGA TGTCATTTTT GAGCTAATGC AAACAGAGAT GCATCACATC 
CAGACCCTGT TCATCATGTC TGAGATCTTC AGGAAAGGCA TGAAAGAGGA GCTGCAGCTG 
GACCACAGCA CCGTGGATAA AATTTTCCCC TGTTTAGATG AGTTGCTTGA AATCCACAGG 
CATTTCTTCT ACAGTATGAA GGAACGAAGG CAGGAATCCT GTGCTGGCAG CGACAGGAAT 
TTTGTGATCG ACCGAATTGG AGATATTTTG GTACAACAGT TTTCAGAAGA AAATGCAAGT 
AAAATGAAGA AAATATATGG AGAATTCTGT TGCCATCATA AAGAAGCTGT TAACCTCTTT 
AAAGAACTCC AGCAGAATAA AAAGTTTCAG AATTTTATTA AGCTCCGAAA TAGTAATCTT 
TTGGCTCGAC GCCGAGGAAT TCCAGAATGC ATTCTGTTGG TCACTCAGCG TATTACAAAA 
TACCCTGTCT TGGTGGAAAG GATATTGCAG TACACAAAGG AAAGAACTGA GGAACATAAA 
GACTTACGCA AAGCGCTTTG CTTAATTAAA GACATGATTG CAACAGTGGA TTTAAAAGTC 
AATGAATATG AGAAAAACCA AAAATGGCTT GAGATCCTAA ATAAGATTGA AAACAAAACA 
TACACGAAGC TCAAAAATGG ACATGTGTTT AGGAAGCAGG CACTGATGAG TGAAGAAAGG 
ACTCTGTTAT ATGATGGCCT TGTTTACTGG AAAACTGCTA CAGGTCGTTT CAAAGATATC 
CTAGCTCTAC TTCTAACTGA TGTGCTGCTC TTTTTACAAG AAAAAGACCA GAAATACATC 
TTTGCAGCCG TTGATCAGAA GCCATCAGTT ATTTCCCTTC AAAAGCTTAT TGCTAGAGAA 
GTTGCTAATG AGGAGAGAGG AATGTTTCTG ATCAGTGCTT CATCTGCTGG TCCTGAGATG 
TATGAAATTC ACACCAATTC CAAGGAGGAA CGCAATAACT GGATGAGACG GATCCAGCAG 
GCTGTAGAAA GTTGTCCTGA AGAAAAAGGG GGAAGGACAA GTGAATCTGA TGAAGACAAG 
AGGAAAGCTG AAGCCAGAGT GGCCAAAATT CAGCAATGTC AAGAAATACT CACTAACCAA 
GACCAACAAA TTTGTGCGTA TTTGGAGGAG AAGCTGCATA TCTATGCTGA ACTTGGAGAA 
CTGAGCGGAT TTGAGGACGT CCATCTAGAG CCCCACCTCC TTATTAAACC TGACCCAGGC 
GAGCCTCCCC AGGCAGCCTC ATTACTGGCA GCAGCACTGA AAGAAGCTGA GAGCCTACAA 
GTTGCAGTGA AGGCCTCACA GATGGGCGCC GTGAGTCAAT CATGTGAGGA CAGTTGTGGA 
GACTCTGTCT TGGCGGACAC ACTCAGTTCT CATGATGTAC CAGGATCACC GACTGCCTCA 
TTAGTCACAG GAGGGAGAGA AGGAAGAGGC TGTTCGGATG TGGATCCCGG GATCCAGGGT 
GTGGTAACCG ACTTGGCCGT CTCTGATGCA GGGGAGAAGG TGGAATGTAG AAATTTTCCA 
GGTTCTTCAC AATCAGAGAT TATACAAGCC ATACAGAATT TAACCCGTCT CTTATACAGC 
CTTCAGGCCG CCTTGACCAT TCAGGACAGC CACATTGAGA TCCACAGGCT GGTTCTCCAG 
CAGCAGGAGG GCCTGTCTCT CGGCCACTCT ATCCTCCGAG GCGGCCCCTT GCAGGACCAG 
AAGTCTCGCG ACGCGGACAG GCAGCATGAG GAGCTGGCCA ATGTGCACCA GCTTCAGCAC 
CAGCTCCAGC AGGAGCAGCG GCGCTGGCTG CGCAGGTGTG AGCAGCAGCA GCGGGCGCAG 
GCGACCAGGG AGAGCTGGCT GCAGGAGCGG GAGCGGGAGT GCCAGTCGCA GGAGGAGCTG 
CTGCTGCGGA GCCGGGGCGA GCTGGACCTC CAGCTCCAGG AGTACCAGCA CAGCCTGGAG 
CGGCTGAGGG AGGGCCAGCG CCTGGTGGAG AGGGAGCAGG CGAGGATGCG GGCCCAGCAG 
AGCCTGCTGG GCCACTGGAA GCACGGCCGG CAGAGGAGCC TGCCCGCGGT GCTCCTTCCG 
GGTGGCCCCG AGGTAATGGA ACTTAATCGA TCTGAGAGTT TATGTCATGA AAACTCATTC 
TTCATCAATG AAGCTTTAGT ACAAATGTCA TTTAACACTT TCAACAAACT GAATCCATCA 
GTTATCCATC AGGATGCCAC TTACCCTACA ACTCAATCTC ATTCTGACTT GGTGAGGACT 
AGTGAACATC AAGTAGACCT CAAGGTGGAC CCTTCTCAGC CTTCGAATGT CAGTCACAAA 
CTGTGGACAG CCGCTGGTTC CGGCCATCAG ATACTTCCTT TCCATGAAAG CAGCAAGGAT 
TCTTGTAAAA ATGGCTCCAG TATGACAAAG TGCAGTTGTA CGTTGACATC TCCCCCGGGA 
CTGTGGACTG GAACCACATC TACTTTGAAA GATTTGGACA CCTCCCACAC TGAGTCCCCA 
ACCCCCCATG ACTCAAATTC ACACCGCCCT CAACTGCAGG CGTTTATAAC AGAAGCAAAG 
CTAAATCTAC CGACAAGGAC AATGACCAGA CAAGATGGGG AAACTGGAGA TGGAGCCAAA 
GAAAATATTG TTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_017009702.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865191.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu95986
Clone ID Related Accession (Same CDS sequence)	XM_017009702.1
Accession Version	XM_017009702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GTATCTTCTC ACAGAGAATC TCTTCTACAC CTGGCTATGA GATGGGGCCT GGCTAAACTT 
TCCCAGTTCT TCTTGTGTCT CCCGGGGGGA GTCCAGGCCT TGGCTTTACC CAACGAAGAG 
GGTGCCACAC CATTAGACTT AGCTTTACGT GAAGGACACT CCAAGCTGGT GGAAGACGTC 
ACAAATTTTC AGGGCAGATG GTCCCCAAGC TTCTCCCGAG TGCAGCTCAG TGAAGAAGCC 
TCCTTGCATT ACATTCACTC ATCGGAAACG CTGACCCTGA CCCTGAACCA CACAGCCGAG 
CATTTGTTGG AGGCAGATAT TAAACTCTTC CGGAAATACT TTTGGGATAG AGCCTTTCTT 
GTCAAGGCCT TTGAGCCAGA AGCCAGGCCA GAGGAAAGAA CAGCTATGCC CTCCAGCGGT 
GCAGAAACTG AAGAAGAGAT TAAGAATTCA GTGTCCAGCA GATCAGCAGC TGAAAAGGAA 
GATATAAAGC GTGTCAAAAG CCTGGTGGTT CAACACAATG AACATGAAGA CCAGCACAGC 
CTAGATTTGG ATCGCTCCTT CGATATCCTA AAAAAATCCA AGCCGCCCTC GACATTGCTT 
GCTGCAGGCC GGCTTTCAGA CATGCTGAAT GGAGGTGATG AAGTCTACGC TAACTGTATG 
GTGATTGATC AGGTTGGTGA TTTGGATATC AGCTATATTA ATATAGAGGG AATCACTGCC 
ACTACCAGCC CTGAATCCAG AGGTTGCACT CTGTGGCCTC AGAGCAGCAA ACACACCCTT 
CCTACAGAAA CCAGTCCCAG TGTGTACCCA CTTAGTGAAA ATGTCGAAGG GACAGCACAC 
ACTGAAGCCC AGCAGTCCTT CATGTCACCA TCAAGTTCGT GTGCTTCCAA CTTGAATCTT 
TCTTTTGGTT GGCATGGATT TGAAAAGGAA CAAAGTCATC TAAAGAAAAG AAGTTCTAGC 
CTTGATGCCT TGGACGCCGA CAGTGAAGGG GAAGGGCATT CTGAGCCATC CCACATCTGT 
TACACTCCAG GGTCTCAGAG CTCCTCAAGA ACTGGGATTC CTAGTGGGGA TGAATTGGAC 
TCTTTTGAGA CTAACACTGA ACCGGATTTT AATATCTCCA GGGCTGAATC CCTTCCTCTA 
TCAAGTAATC TACAGTCGAA GGAATCACTG CTTTCTGGAG TTCGCTCACG TTCTTATTCT 
TGCTCATCAC CCAAAATTTC TTTAGGAAAA ACTCGTTTGG TGCGTGAATT AACAGTATGC 
AGTTCAAGTG AAGAGCAAAG AGCTTACAGC TTATCGGAGC CACCAAGAGA AAACAGAATT 
CAGGAAGAAG AATGGGATAA ATACATCATA CCTGCCAAAT CAGAGTCTGA AAAATATAAA 
GTGAGTCGAA CTTTCAGTTT CCTCATGAAT AGGATGACTA GCCCTCGGAA TAAATCAAAG 
ACAAAAAGCA AGGATGCCAA AGATAAAGAG AAGCTGAATC GACATCAGTT TGCCCCAGGA 
ACATTCTCTG GGGTTCTGCA GTGTTTGGTT TGTGATAAAA CACTCCTGGG GAAAGAGTCA 
CTGCAGTGTT CTAACTGTAA TGCAAATGTG CACAAAGGTT GTAAAGATGC TGCGCCTGCA 
TGCACCAAGA AATTCCAAGA GAAATATAAC AAGAACAAAC CACAGACCAT CCTTGGAAAT 
TCTTCATTTA GAGACATCCC ACAGCCTGGT CTCTCCTTGC ACCCTTCTTC CTCCGTGCCT 
GTTGGATTGC CGACTGGAAG GAGGGAGACT GTGGGACAGG TCCATCCATT GTCCAGAAGT 
GTTCCAGGCA CCACCTTGGA AAGCTTCAGG AGGTCAGCCA CATCCTTGGA GTCTGAGAGT 
GACCATAACA GCTGCAGAAG CAGGTCTCAT TCTGATGAGC TGCTACAGTC CATGGGCTCT 
TCTCCCTCTA CAGAGTCTTT CATAATGGAA GATGTTGTGG ATTCTTCTCT GTGGAGTGAC 
CTCAGCAGTG ATGCCCAGGA GTTTGAAGCA GAATCTTGGA GTCTTGTGGT GGATCCCTCA 
TTTTGTAATA GGCAGGAGAA GGATGTCATC AAAAGACAGG ATGTCATTTT TGAGCTAATG 
CAAACAGAGA TGCATCACAT CCAGACCCTG TTCATCATGT CTGAGATCTT CAGGAAAGGC 
ATGAAAGAGG AGCTGCAGCT GGACCACAGC ACCGTGGATA AAATTTTCCC CTGTTTAGAT 
GAGTTGCTTG AAATCCACAG GCATTTCTTC TACAGTATGA AGGAACGAAG GCAGGAATCC 
TGTGCTGGCA GCGACAGGAA TTTTGTGATC GACCGAATTG GAGATATTTT GGTACAACAG 
TTTTCAGAAG AAAATGCAAG TAAAATGAAG AAAATATATG GAGAATTCTG TTGCCATCAT 
AAAGAAGCTG TTAACCTCTT TAAAGAACTC CAGCAGAATA AAAAGTTTCA GAATTTTATT 
AAGCTCCGAA ATAGTAATCT TTTGGCTCGA CGCCGAGGAA TTCCAGAATG CATTCTGTTG 
GTCACTCAGC GTATTACAAA ATACCCTGTC TTGGTGGAAA GGATATTGCA GTACACAAAG 
GAAAGAACTG AGGAACATAA AGACTTACGC AAAGCGCTTT GCTTAATTAA AGACATGATT 
GCAACAGTGG ATTTAAAAGT CAATGAATAT GAGAAAAACC AAAAATGGCT TGAGATCCTA 
AATAAGATTG AAAACAAAAC ATACACGAAG CTCAAAAATG GACATGTGTT TAGGAAGCAG 
GCACTGATGA GTGAAGAAAG GACTCTGTTA TATGATGGCC TTGTTTACTG GAAAACTGCT 
ACAGGTCGTT TCAAAGATAT CCTAGCTCTA CTTCTAACTG ATGTGCTGCT CTTTTTACAA 
GAAAAAGACC AGAAATACAT CTTTGCAGCC GTTGATCAGA AGCCATCAGT TATTTCCCTT 
CAAAAGCTTA TTGCTAGAGA AGTTGCTAAT GAGGAGAGAG GAATGTTTCT GATCAGTGCT 
TCATCTGCTG GTCCTGAGAT GTATGAAATT CACACCAATT CCAAGGAGGA ACGCAATAAC 
TGGATGAGAC GGATCCAGCA GGCTGTAGAA AGTTGTCCTG AAGAAAAAGG GGGAAGGACA 
AGTGAATCTG ATGAAGACAA GAGGAAAGCT GAAGCCAGAG TGGCCAAAAT TCAGCAATGT 
CAAGAAATAC TCACTAACCA AGACCAACAA ATTTGTGCGT ATTTGGAGGA GAAGCTGCAT 
ATCTATGCTG AACTTGGAGA ACTGAGCGGA TTTGAGGACG TCCATCTAGA GCCCCACCTC 
CTTATTAAAC CTGACCCAGG CGAGCCTCCC CAGGCAGCCT CATTACTGGC AGCAGCACTG 
AAAGAAGCTG AGAGCCTACA AGTTGCAGTG AAGGCCTCAC AGATGGGCGC CGTGAGTCAA 
TCATGTGAGG ACAGTTGTGG AGACTCTGTC TTGGCGGACA CACTCAGTTC TCATGATGTA 
CCAGGATCAC CGACTGCCTC ATTAGTCACA GGAGGGAGAG AAGGAAGAGG CTGTTCGGAT 
GTGGATCCCG GGATCCAGGG TGTGGTAACC GACTTGGCCG TCTCTGATGC AGGGGAGAAG 
GTGGAATGTA GAAATTTTCC AGGTTCTTCA CAATCAGAGA TTATACAAGC CATACAGAAT 
TTAACCCGTC TCTTATACAG CCTTCAGGCC GCCTTGACCA TTCAGGACAG CCACATTGAG 
ATCCACAGGC TGGTTCTCCA GCAGCAGGAG GGCCTGTCTC TCGGCCACTC TATCCTCCGA 
GGCGGCCCCT TGCAGGACCA GAAGTCTCGC GACGCGGACA GGCAGCATGA GGAGCTGGCC 
AATGTGCACC AGCTTCAGCA CCAGCTCCAG CAGGAGCAGC GGCGCTGGCT GCGCAGGTGT 
GAGCAGCAGC AGCGGGCGCA GGCGACCAGG GAGAGCTGGC TGCAGGAGCG GGAGCGGGAG 
TGCCAGTCGC AGGAGGAGCT GCTGCTGCGG AGCCGGGGCG AGCTGGACCT CCAGCTCCAG 
GAGTACCAGC ACAGCCTGGA GCGGCTGAGG GAGGGCCAGC GCCTGGTGGA GAGGGAGCAG 
GCGAGGATGC GGGCCCAGCA GAGCCTGCTG GGCCACTGGA AGCACGGCCG GCAGAGGAGC 
CTGCCCGCGG TGCTCCTTCC GGGTGGCCCC GAGGTAATGG AACTTAATCG ATCTGAGAGT 
TTATGTCATG AAAACTCATT CTTCATCAAT GAAGCTTTAG TACAAATGTC ATTTAACACT 
TTCAACAAAC TGAATCCATC AGTTATCCAT CAGGATGCCA CTTACCCTAC AACTCAATCT 
CATTCTGACT TGGTGAGGAC TAGTGAACAT CAAGTAGACC TCAAGGTGGA CCCTTCTCAG 
CCTTCGAATG TCAGTCACAA ACTGTGGACA GCCGCTGGTT CCGGCCATCA GATACTTCCT 
TTCCATGAAA GCAGCAAGGA TTCTTGTAAA AATGGCTCCA GTATGACAAA GTGCAGTTGT 
ACGTTGACAT CTCCCCCGGG ACTGTGGACT GGAACCACAT CTACTTTGAA AGATTTGGAC 
ACCTCCCACA CTGAGTCCCC AACCCCCCAT GACTCAAATT CACACCGCCC TCAACTGCAG 
GCGTTTATAA CAGAAGCAAA GCTAAATCTA CCGACAAGGA CAATGACCAG ACAAGATGGG 
GAAACTGGAG ATGGAGCCAA AGAAAATATT GTTTACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865191.1
CDS	156..5057
Translation	MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	XM_017009702.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009702.1

ORF Insert Sequence:

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GTATCTTCTC ACAGAGAATC TCTTCTACAC CTGGCTATGA GATGGGGCCT GGCTAAACTT 
TCCCAGTTCT TCTTGTGTCT CCCGGGGGGA GTCCAGGCCT TGGCTTTACC CAACGAAGAG 
GGTGCCACAC CATTAGACTT AGCTTTACGT GAAGGACACT CCAAGCTGGT GGAAGACGTC 
ACAAATTTTC AGGGCAGATG GTCCCCAAGC TTCTCCCGAG TGCAGCTCAG TGAAGAAGCC 
TCCTTGCATT ACATTCACTC ATCGGAAACG CTGACCCTGA CCCTGAACCA CACAGCCGAG 
CATTTGTTGG AGGCAGATAT TAAACTCTTC CGGAAATACT TTTGGGATAG AGCCTTTCTT 
GTCAAGGCCT TTGAGCCAGA AGCCAGGCCA GAGGAAAGAA CAGCTATGCC CTCCAGCGGT 
GCAGAAACTG AAGAAGAGAT TAAGAATTCA GTGTCCAGCA GATCAGCAGC TGAAAAGGAA 
GATATAAAGC GTGTCAAAAG CCTGGTGGTT CAACACAATG AACATGAAGA CCAGCACAGC 
CTAGATTTGG ATCGCTCCTT CGATATCCTA AAAAAATCCA AGCCGCCCTC GACATTGCTT 
GCTGCAGGCC GGCTTTCAGA CATGCTGAAT GGAGGTGATG AAGTCTACGC TAACTGTATG 
GTGATTGATC AGGTTGGTGA TTTGGATATC AGCTATATTA ATATAGAGGG AATCACTGCC 
ACTACCAGCC CTGAATCCAG AGGTTGCACT CTGTGGCCTC AGAGCAGCAA ACACACCCTT 
CCTACAGAAA CCAGTCCCAG TGTGTACCCA CTTAGTGAAA ATGTCGAAGG GACAGCACAC 
ACTGAAGCCC AGCAGTCCTT CATGTCACCA TCAAGTTCGT GTGCTTCCAA CTTGAATCTT 
TCTTTTGGTT GGCATGGATT TGAAAAGGAA CAAAGTCATC TAAAGAAAAG AAGTTCTAGC 
CTTGATGCCT TGGACGCCGA CAGTGAAGGG GAAGGGCATT CTGAGCCATC CCACATCTGT 
TACACTCCAG GGTCTCAGAG CTCCTCAAGA ACTGGGATTC CTAGTGGGGA TGAATTGGAC 
TCTTTTGAGA CTAACACTGA ACCGGATTTT AATATCTCCA GGGCTGAATC CCTTCCTCTA 
TCAAGTAATC TACAGTCGAA GGAATCACTG CTTTCTGGAG TTCGCTCACG TTCTTATTCT 
TGCTCATCAC CCAAAATTTC TTTAGGAAAA ACTCGTTTGG TGCGTGAATT AACAGTATGC 
AGTTCAAGTG AAGAGCAAAG AGCTTACAGC TTATCGGAGC CACCAAGAGA AAACAGAATT 
CAGGAAGAAG AATGGGATAA ATACATCATA CCTGCCAAAT CAGAGTCTGA AAAATATAAA 
GTGAGTCGAA CTTTCAGTTT CCTCATGAAT AGGATGACTA GCCCTCGGAA TAAATCAAAG 
ACAAAAAGCA AGGATGCCAA AGATAAAGAG AAGCTGAATC GACATCAGTT TGCCCCAGGA 
ACATTCTCTG GGGTTCTGCA GTGTTTGGTT TGTGATAAAA CACTCCTGGG GAAAGAGTCA 
CTGCAGTGTT CTAACTGTAA TGCAAATGTG CACAAAGGTT GTAAAGATGC TGCGCCTGCA 
TGCACCAAGA AATTCCAAGA GAAATATAAC AAGAACAAAC CACAGACCAT CCTTGGAAAT 
TCTTCATTTA GAGACATCCC ACAGCCTGGT CTCTCCTTGC ACCCTTCTTC CTCCGTGCCT 
GTTGGATTGC CGACTGGAAG GAGGGAGACT GTGGGACAGG TCCATCCATT GTCCAGAAGT 
GTTCCAGGCA CCACCTTGGA AAGCTTCAGG AGGTCAGCCA CATCCTTGGA GTCTGAGAGT 
GACCATAACA GCTGCAGAAG CAGGTCTCAT TCTGATGAGC TGCTACAGTC CATGGGCTCT 
TCTCCCTCTA CAGAGTCTTT CATAATGGAA GATGTTGTGG ATTCTTCTCT GTGGAGTGAC 
CTCAGCAGTG ATGCCCAGGA GTTTGAAGCA GAATCTTGGA GTCTTGTGGT GGATCCCTCA 
TTTTGTAATA GGCAGGAGAA GGATGTCATC AAAAGACAGG ATGTCATTTT TGAGCTAATG 
CAAACAGAGA TGCATCACAT CCAGACCCTG TTCATCATGT CTGAGATCTT CAGGAAAGGC 
ATGAAAGAGG AGCTGCAGCT GGACCACAGC ACCGTGGATA AAATTTTCCC CTGTTTAGAT 
GAGTTGCTTG AAATCCACAG GCATTTCTTC TACAGTATGA AGGAACGAAG GCAGGAATCC 
TGTGCTGGCA GCGACAGGAA TTTTGTGATC GACCGAATTG GAGATATTTT GGTACAACAG 
TTTTCAGAAG AAAATGCAAG TAAAATGAAG AAAATATATG GAGAATTCTG TTGCCATCAT 
AAAGAAGCTG TTAACCTCTT TAAAGAACTC CAGCAGAATA AAAAGTTTCA GAATTTTATT 
AAGCTCCGAA ATAGTAATCT TTTGGCTCGA CGCCGAGGAA TTCCAGAATG CATTCTGTTG 
GTCACTCAGC GTATTACAAA ATACCCTGTC TTGGTGGAAA GGATATTGCA GTACACAAAG 
GAAAGAACTG AGGAACATAA AGACTTACGC AAAGCGCTTT GCTTAATTAA AGACATGATT 
GCAACAGTGG ATTTAAAAGT CAATGAATAT GAGAAAAACC AAAAATGGCT TGAGATCCTA 
AATAAGATTG AAAACAAAAC ATACACGAAG CTCAAAAATG GACATGTGTT TAGGAAGCAG 
GCACTGATGA GTGAAGAAAG GACTCTGTTA TATGATGGCC TTGTTTACTG GAAAACTGCT 
ACAGGTCGTT TCAAAGATAT CCTAGCTCTA CTTCTAACTG ATGTGCTGCT CTTTTTACAA 
GAAAAAGACC AGAAATACAT CTTTGCAGCC GTTGATCAGA AGCCATCAGT TATTTCCCTT 
CAAAAGCTTA TTGCTAGAGA AGTTGCTAAT GAGGAGAGAG GAATGTTTCT GATCAGTGCT 
TCATCTGCTG GTCCTGAGAT GTATGAAATT CACACCAATT CCAAGGAGGA ACGCAATAAC 
TGGATGAGAC GGATCCAGCA GGCTGTAGAA AGTTGTCCTG AAGAAAAAGG GGGAAGGACA 
AGTGAATCTG ATGAAGACAA GAGGAAAGCT GAAGCCAGAG TGGCCAAAAT TCAGCAATGT 
CAAGAAATAC TCACTAACCA AGACCAACAA ATTTGTGCGT ATTTGGAGGA GAAGCTGCAT 
ATCTATGCTG AACTTGGAGA ACTGAGCGGA TTTGAGGACG TCCATCTAGA GCCCCACCTC 
CTTATTAAAC CTGACCCAGG CGAGCCTCCC CAGGCAGCCT CATTACTGGC AGCAGCACTG 
AAAGAAGCTG AGAGCCTACA AGTTGCAGTG AAGGCCTCAC AGATGGGCGC CGTGAGTCAA 
TCATGTGAGG ACAGTTGTGG AGACTCTGTC TTGGCGGACA CACTCAGTTC TCATGATGTA 
CCAGGATCAC CGACTGCCTC ATTAGTCACA GGAGGGAGAG AAGGAAGAGG CTGTTCGGAT 
GTGGATCCCG GGATCCAGGG TGTGGTAACC GACTTGGCCG TCTCTGATGC AGGGGAGAAG 
GTGGAATGTA GAAATTTTCC AGGTTCTTCA CAATCAGAGA TTATACAAGC CATACAGAAT 
TTAACCCGTC TCTTATACAG CCTTCAGGCC GCCTTGACCA TTCAGGACAG CCACATTGAG 
ATCCACAGGC TGGTTCTCCA GCAGCAGGAG GGCCTGTCTC TCGGCCACTC TATCCTCCGA 
GGCGGCCCCT TGCAGGACCA GAAGTCTCGC GACGCGGACA GGCAGCATGA GGAGCTGGCC 
AATGTGCACC AGCTTCAGCA CCAGCTCCAG CAGGAGCAGC GGCGCTGGCT GCGCAGGTGT 
GAGCAGCAGC AGCGGGCGCA GGCGACCAGG GAGAGCTGGC TGCAGGAGCG GGAGCGGGAG 
TGCCAGTCGC AGGAGGAGCT GCTGCTGCGG AGCCGGGGCG AGCTGGACCT CCAGCTCCAG 
GAGTACCAGC ACAGCCTGGA GCGGCTGAGG GAGGGCCAGC GCCTGGTGGA GAGGGAGCAG 
GCGAGGATGC GGGCCCAGCA GAGCCTGCTG GGCCACTGGA AGCACGGCCG GCAGAGGAGC 
CTGCCCGCGG TGCTCCTTCC GGGTGGCCCC GAGGTAATGG AACTTAATCG ATCTGAGAGT 
TTATGTCATG AAAACTCATT CTTCATCAAT GAAGCTTTAG TACAAATGTC ATTTAACACT 
TTCAACAAAC TGAATCCATC AGTTATCCAT CAGGATGCCA CTTACCCTAC AACTCAATCT 
CATTCTGACT TGGTGAGGAC TAGTGAACAT CAAGTAGACC TCAAGGTGGA CCCTTCTCAG 
CCTTCGAATG TCAGTCACAA ACTGTGGACA GCCGCTGGTT CCGGCCATCA GATACTTCCT 
TTCCATGAAA GCAGCAAGGA TTCTTGTAAA AATGGCTCCA GTATGACAAA GTGCAGTTGT 
ACGTTGACAT CTCCCCCGGG ACTGTGGACT GGAACCACAT CTACTTTGAA AGATTTGGAC 
ACCTCCCACA CTGAGTCCCC AACCCCCCAT GACTCAAATT CACACCGCCC TCAACTGCAG 
GCGTTTATAA CAGAAGCAAA GCTAAATCTA CCGACAAGGA CAATGACCAG ACAAGATGGG 
GAAACTGGAG ATGGAGCCAA AGAAAATATT GTTTACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( NM_001177693.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171164.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 2, mRNA.

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CloneID	OHu00087
Clone ID Related Accession (Same CDS sequence)	NM_001177693.1
Accession Version	NM_001177693.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK094713.1, BC171850.1, AC093283.3 and CN478799.1. Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC171850.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGAT TTGGACACCT CCCACACTGA GTCCCCAACC 
CCCCATGACT CAAATTCACA CCGCCCTCAA CTGCAGGCGT TTATAACAGA AGCAAAGCTA 
AATCTACCGA CAAGGACAAT GACCAGACAA GATGGGGAAA CTGGAGATGG AGCCAAAGAA 
AATATTGTTT ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171164.1
CDS	177..5294
Translation	MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	NM_001177693.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001177693.1

ORF Insert Sequence:

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGAT TTGGACACCT CCCACACTGA GTCCCCAACC 
CCCCATGACT CAAATTCACA CCGCCCTCAA CTGCAGGCGT TTATAACAGA AGCAAAGCTA 
AATCTACCGA CAAGGACAAT GACCAGACAA GATGGGGAAA CTGGAGATGG AGCCAAAGAA 
AATATTGTTT ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( NM_001244364.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001231293.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 3, mRNA.

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CloneID	OHu11947
Clone ID Related Accession (Same CDS sequence)	NM_001244364.2 , NM_001244364.1
Accession Version	NM_001244364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-13
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304761.1, AC093283.3, BG696752.1 and AK025470.1. On Jun 2, 2019 this sequence version replaced NM_001244364.1. Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCTG ACTCCGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTTCC CAAAATGAAG 
ATTCGAAGAA GGACGTCCAA ACAAGATCGC TCCTTCGATA TCCTAAAAAA ATCCAAGCCG 
CCCTCGACAT TGCTTGCTGC AGGCCGGCTT TCAGACATGC TGAATGGAGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGATCAGGTT GGTGATTTGG ATATCAGCTA TATTAATATA 
GAGGGAATCA CTGCCACTAC CAGCCCTGAA TCCAGAGGTT GCACTCTGTG GCCTCAGAGC 
AGCAAACACA CCCTTCCTAC AGAAACCAGT CCCAGTGTGT ACCCACTTAG TGAAAATGTC 
GAAGGGACAG CACACACTGA AGCCCAGCAG TCCTTCATGT CACCATCAAG TTCGTGTGCT 
TCCAACTTGA ATCTTTCTTT TGGTTGGCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AAAAGAAGTT CTAGCCTTGA TGCCTTGGAC GCCGACAGTG AAGGGGAAGG GCATTCTGAG 
CCATCCCACA TCTGTTACAC TCCAGGGTCT CAGAGCTCCT CAAGAACTGG GATTCCTAGT 
GGGGATGAAT TGGACTCTTT TGAGACTAAC ACTGAACCGG ATTTTAATAT CTCCAGGGCT 
GAATCCCTTC CTCTATCAAG TAATCTACAG TCGAAGGAAT CACTGCTTTC TGGAGTTCGC 
TCACGTTCTT ATTCTTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG TTTGGTGCGT 
GAATTAACAG TATGCAGTTC AAGTGAAGAG CAAAGAGCTT ACAGCTTATC GGAGCCACCA 
AGAGAAAACA GAATTCAGGA AGAAGAATGG GATAAATACA TCATACCTGC CAAATCAGAG 
TCTGAAAAAT ATAAAGTGAG TCGAACTTTC AGTTTCCTCA TGAATAGGAT GACTAGCCCT 
CGGAATAAAT CAAAGACAAA AAGCAAGGAT GCCAAAGATA AAGAGAAGCT GAATCGACAT 
CAGTTTGCCC CAGGAACATT CTCTGGGGTT CTGCAGTGTT TGGTTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTGCA GTGTTCTAAC TGTAATGCAA ATGTGCACAA AGGTTGTAAA 
GATGCTGCGC CTGCATGCAC CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTTAGAGAC ATCCCACAGC CTGGTCTCTC CTTGCACCCT 
TCTTCCTCCG TGCCTGTTGG ATTGCCGACT GGAAGGAGGG AGACTGTGGG ACAGGTCCAT 
CCATTGTCCA GAAGTGTTCC AGGCACCACC TTGGAAAGCT TCAGGAGGTC AGCCACATCC 
TTGGAGTCTG AGAGTGACCA TAACAGCTGC AGAAGCAGGT CTCATTCTGA TGAGCTGCTA 
CAGTCCATGG GCTCTTCTCC CTCTACAGAG TCTTTCATAA TGGAAGATGT TGTGGATTCT 
TCTCTGTGGA GTGACCTCAG CAGTGATGCC CAGGAGTTTG AAGCAGAATC TTGGAGTCTT 
GTGGTGGATC CCTCATTTTG TAATAGGCAG GAGAAGGATG TCATCAAAAG ACAGGATGTC 
ATTTTTGAGC TAATGCAAAC AGAGATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGCATGAA AGAGGAGCTG CAGCTGGACC ACAGCACCGT GGATAAAATT 
TTCCCCTGTT TAGATGAGTT GCTTGAAATC CACAGGCATT TCTTCTACAG TATGAAGGAA 
CGAAGGCAGG AATCCTGTGC TGGCAGCGAC AGGAATTTTG TGATCGACCG AATTGGAGAT 
ATTTTGGTAC AACAGTTTTC AGAAGAAAAT GCAAGTAAAA TGAAGAAAAT ATATGGAGAA 
TTCTGTTGCC ATCATAAAGA AGCTGTTAAC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTTCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGTTGGTCAC TCAGCGTATT ACAAAATACC CTGTCTTGGT GGAAAGGATA 
TTGCAGTACA CAAAGGAAAG AACTGAGGAA CATAAAGACT TACGCAAAGC GCTTTGCTTA 
ATTAAAGACA TGATTGCAAC AGTGGATTTA AAAGTCAATG AATATGAGAA AAACCAAAAA 
TGGCTTGAGA TCCTAAATAA GATTGAAAAC AAAACATACA CGAAGCTCAA AAATGGACAT 
GTGTTTAGGA AGCAGGCACT GATGAGTGAA GAAAGGACTC TGTTATATGA TGGCCTTGTT 
TACTGGAAAA CTGCTACAGG TCGTTTCAAA GATATCCTAG CTCTACTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCCGTTGA TCAGAAGCCA 
TCAGTTATTT CCCTTCAAAA GCTTATTGCT AGAGAAGTTG CTAATGAGGA GAGAGGAATG 
TTTCTGATCA GTGCTTCATC TGCTGGTCCT GAGATGTATG AAATTCACAC CAATTCCAAG 
GAGGAACGCA ATAACTGGAT GAGACGGATC CAGCAGGCTG TAGAAAGTTG TCCTGAAGAA 
AAAGGGGGAA GGACAAGTGA ATCTGATGAA GACAAGAGGA AAGCTGAAGC CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCACT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATCTA TGCTGAACTT GGAGAACTGA GCGGATTTGA GGACGTCCAT 
CTAGAGCCCC ACCTCCTTAT TAAACCTGAC CCAGGCGAGC CTCCCCAGGC AGCCTCATTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTACAAGTTG CAGTGAAGGC CTCACAGATG 
GGCGCCGTGA GTCAATCATG TGAGGACAGT TGTGGAGACT CTGTCTTGGC GGACACACTC 
AGTTCTCATG ATGTACCAGG ATCACCGACT GCCTCATTAG TCACAGGAGG GAGAGAAGGA 
AGAGGCTGTT CGGATGTGGA TCCCGGGATC CAGGGTGTGG TAACCGACTT GGCCGTCTCT 
GATGCAGGGG AGAAGGTGGA ATGTAGAAAT TTTCCAGGTT CTTCACAATC AGAGATTATA 
CAAGCCATAC AGAATTTAAC CCGTCTCTTA TACAGCCTTC AGGCCGCCTT GACCATTCAG 
GACAGCCACA TTGAGATCCA CAGGCTGGTT CTCCAGCAGC AGGAGGGCCT GTCTCTCGGC 
CACTCTATCC TCCGAGGCGG CCCCTTGCAG GACCAGAAGT CTCGCGACGC GGACAGGCAG 
CATGAGGAGC TGGCCAATGT GCACCAGCTT CAGCACCAGC TCCAGCAGGA GCAGCGGCGC 
TGGCTGCGCA GGTGTGAGCA GCAGCAGCGG GCGCAGGCGA CCAGGGAGAG CTGGCTGCAG 
GAGCGGGAGC GGGAGTGCCA GTCGCAGGAG GAGCTGCTGC TGCGGAGCCG GGGCGAGCTG 
GACCTCCAGC TCCAGGAGTA CCAGCACAGC CTGGAGCGGC TGAGGGAGGG CCAGCGCCTG 
GTGGAGAGGG AGCAGGCGAG GATGCGGGCC CAGCAGAGCC TGCTGGGCCA CTGGAAGCAC 
GGCCGGCAGA GGAGCCTGCC CGCGGTGCTC CTTCCGGGTG GCCCCGAGGT AATGGAACTT 
AATCGATCTG AGAGTTTATG TCATGAAAAC TCATTCTTCA TCAATGAAGC TTTAGTACAA 
ATGTCATTTA ACACTTTCAA CAAACTGAAT CCATCAGTTA TCCATCAGGA TGCCACTTAC 
CCTACAACTC AATCTCATTC TGACTTGGTG AGGACTAGTG AACATCAAGT AGACCTCAAG 
GTGGACCCTT CTCAGCCTTC GAATGTCAGT CACAAACTGT GGACAGCCGC TGGTTCCGGC 
CATCAGATAC TTCCTTTCCA TGAAAGCAGC AAGGATTCTT GTAAAAATGA TTTGGACACC 
TCCCACACTG AGTCCCCAAC CCCCCATGAC TCAAATTCAC ACCGCCCTCA ACTGCAGGCG 
TTTATAACAG AAGCAAAGCT AAATCTACCG ACAAGGACAA TGACCAGACA AGATGGGGAA 
ACTGGAGATG GAGCCAAAGA AAATATTGTT TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231293.1
CDS	44..4222
Translation	MDSDSDSPFNYSWPSFPKMKIRRRTSKQDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	NM_001244364.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244364.2

ORF Insert Sequence:

ATGGATTCTG ACTCCGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTTCC CAAAATGAAG 
ATTCGAAGAA GGACGTCCAA ACAAGATCGC TCCTTCGATA TCCTAAAAAA ATCCAAGCCG 
CCCTCGACAT TGCTTGCTGC AGGCCGGCTT TCAGACATGC TGAATGGAGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGATCAGGTT GGTGATTTGG ATATCAGCTA TATTAATATA 
GAGGGAATCA CTGCCACTAC CAGCCCTGAA TCCAGAGGTT GCACTCTGTG GCCTCAGAGC 
AGCAAACACA CCCTTCCTAC AGAAACCAGT CCCAGTGTGT ACCCACTTAG TGAAAATGTC 
GAAGGGACAG CACACACTGA AGCCCAGCAG TCCTTCATGT CACCATCAAG TTCGTGTGCT 
TCCAACTTGA ATCTTTCTTT TGGTTGGCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AAAAGAAGTT CTAGCCTTGA TGCCTTGGAC GCCGACAGTG AAGGGGAAGG GCATTCTGAG 
CCATCCCACA TCTGTTACAC TCCAGGGTCT CAGAGCTCCT CAAGAACTGG GATTCCTAGT 
GGGGATGAAT TGGACTCTTT TGAGACTAAC ACTGAACCGG ATTTTAATAT CTCCAGGGCT 
GAATCCCTTC CTCTATCAAG TAATCTACAG TCGAAGGAAT CACTGCTTTC TGGAGTTCGC 
TCACGTTCTT ATTCTTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG TTTGGTGCGT 
GAATTAACAG TATGCAGTTC AAGTGAAGAG CAAAGAGCTT ACAGCTTATC GGAGCCACCA 
AGAGAAAACA GAATTCAGGA AGAAGAATGG GATAAATACA TCATACCTGC CAAATCAGAG 
TCTGAAAAAT ATAAAGTGAG TCGAACTTTC AGTTTCCTCA TGAATAGGAT GACTAGCCCT 
CGGAATAAAT CAAAGACAAA AAGCAAGGAT GCCAAAGATA AAGAGAAGCT GAATCGACAT 
CAGTTTGCCC CAGGAACATT CTCTGGGGTT CTGCAGTGTT TGGTTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTGCA GTGTTCTAAC TGTAATGCAA ATGTGCACAA AGGTTGTAAA 
GATGCTGCGC CTGCATGCAC CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTTAGAGAC ATCCCACAGC CTGGTCTCTC CTTGCACCCT 
TCTTCCTCCG TGCCTGTTGG ATTGCCGACT GGAAGGAGGG AGACTGTGGG ACAGGTCCAT 
CCATTGTCCA GAAGTGTTCC AGGCACCACC TTGGAAAGCT TCAGGAGGTC AGCCACATCC 
TTGGAGTCTG AGAGTGACCA TAACAGCTGC AGAAGCAGGT CTCATTCTGA TGAGCTGCTA 
CAGTCCATGG GCTCTTCTCC CTCTACAGAG TCTTTCATAA TGGAAGATGT TGTGGATTCT 
TCTCTGTGGA GTGACCTCAG CAGTGATGCC CAGGAGTTTG AAGCAGAATC TTGGAGTCTT 
GTGGTGGATC CCTCATTTTG TAATAGGCAG GAGAAGGATG TCATCAAAAG ACAGGATGTC 
ATTTTTGAGC TAATGCAAAC AGAGATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGCATGAA AGAGGAGCTG CAGCTGGACC ACAGCACCGT GGATAAAATT 
TTCCCCTGTT TAGATGAGTT GCTTGAAATC CACAGGCATT TCTTCTACAG TATGAAGGAA 
CGAAGGCAGG AATCCTGTGC TGGCAGCGAC AGGAATTTTG TGATCGACCG AATTGGAGAT 
ATTTTGGTAC AACAGTTTTC AGAAGAAAAT GCAAGTAAAA TGAAGAAAAT ATATGGAGAA 
TTCTGTTGCC ATCATAAAGA AGCTGTTAAC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTTCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGTTGGTCAC TCAGCGTATT ACAAAATACC CTGTCTTGGT GGAAAGGATA 
TTGCAGTACA CAAAGGAAAG AACTGAGGAA CATAAAGACT TACGCAAAGC GCTTTGCTTA 
ATTAAAGACA TGATTGCAAC AGTGGATTTA AAAGTCAATG AATATGAGAA AAACCAAAAA 
TGGCTTGAGA TCCTAAATAA GATTGAAAAC AAAACATACA CGAAGCTCAA AAATGGACAT 
GTGTTTAGGA AGCAGGCACT GATGAGTGAA GAAAGGACTC TGTTATATGA TGGCCTTGTT 
TACTGGAAAA CTGCTACAGG TCGTTTCAAA GATATCCTAG CTCTACTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCCGTTGA TCAGAAGCCA 
TCAGTTATTT CCCTTCAAAA GCTTATTGCT AGAGAAGTTG CTAATGAGGA GAGAGGAATG 
TTTCTGATCA GTGCTTCATC TGCTGGTCCT GAGATGTATG AAATTCACAC CAATTCCAAG 
GAGGAACGCA ATAACTGGAT GAGACGGATC CAGCAGGCTG TAGAAAGTTG TCCTGAAGAA 
AAAGGGGGAA GGACAAGTGA ATCTGATGAA GACAAGAGGA AAGCTGAAGC CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCACT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATCTA TGCTGAACTT GGAGAACTGA GCGGATTTGA GGACGTCCAT 
CTAGAGCCCC ACCTCCTTAT TAAACCTGAC CCAGGCGAGC CTCCCCAGGC AGCCTCATTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTACAAGTTG CAGTGAAGGC CTCACAGATG 
GGCGCCGTGA GTCAATCATG TGAGGACAGT TGTGGAGACT CTGTCTTGGC GGACACACTC 
AGTTCTCATG ATGTACCAGG ATCACCGACT GCCTCATTAG TCACAGGAGG GAGAGAAGGA 
AGAGGCTGTT CGGATGTGGA TCCCGGGATC CAGGGTGTGG TAACCGACTT GGCCGTCTCT 
GATGCAGGGG AGAAGGTGGA ATGTAGAAAT TTTCCAGGTT CTTCACAATC AGAGATTATA 
CAAGCCATAC AGAATTTAAC CCGTCTCTTA TACAGCCTTC AGGCCGCCTT GACCATTCAG 
GACAGCCACA TTGAGATCCA CAGGCTGGTT CTCCAGCAGC AGGAGGGCCT GTCTCTCGGC 
CACTCTATCC TCCGAGGCGG CCCCTTGCAG GACCAGAAGT CTCGCGACGC GGACAGGCAG 
CATGAGGAGC TGGCCAATGT GCACCAGCTT CAGCACCAGC TCCAGCAGGA GCAGCGGCGC 
TGGCTGCGCA GGTGTGAGCA GCAGCAGCGG GCGCAGGCGA CCAGGGAGAG CTGGCTGCAG 
GAGCGGGAGC GGGAGTGCCA GTCGCAGGAG GAGCTGCTGC TGCGGAGCCG GGGCGAGCTG 
GACCTCCAGC TCCAGGAGTA CCAGCACAGC CTGGAGCGGC TGAGGGAGGG CCAGCGCCTG 
GTGGAGAGGG AGCAGGCGAG GATGCGGGCC CAGCAGAGCC TGCTGGGCCA CTGGAAGCAC 
GGCCGGCAGA GGAGCCTGCC CGCGGTGCTC CTTCCGGGTG GCCCCGAGGT AATGGAACTT 
AATCGATCTG AGAGTTTATG TCATGAAAAC TCATTCTTCA TCAATGAAGC TTTAGTACAA 
ATGTCATTTA ACACTTTCAA CAAACTGAAT CCATCAGTTA TCCATCAGGA TGCCACTTAC 
CCTACAACTC AATCTCATTC TGACTTGGTG AGGACTAGTG AACATCAAGT AGACCTCAAG 
GTGGACCCTT CTCAGCCTTC GAATGTCAGT CACAAACTGT GGACAGCCGC TGGTTCCGGC 
CATCAGATAC TTCCTTTCCA TGAAAGCAGC AAGGATTCTT GTAAAAATGA TTTGGACACC 
TCCCACACTG AGTCCCCAAC CCCCCATGAC TCAAATTCAC ACCGCCCTCA ACTGCAGGCG 
TTTATAACAG AAGCAAAGCT AAATCTACCG ACAAGGACAA TGACCAGACA AGATGGGGAA 
ACTGGAGATG GAGCCAAAGA AAATATTGTT TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( NM_001244364.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001231293.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 3, mRNA.

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CloneID	OHu11947
Clone ID Related Accession (Same CDS sequence)	NM_001244364.2 , NM_001244364.1
Accession Version	NM_001244364.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK304761.1, AC093283.3, BG696752.1 and AK025470.1. Transcript Variant: This variant (3) has multiple differences, compared to variant 1. These differences result in a distinct 5' UTR and cause translation initiation at an alternate start codon, compared to variant 1. The encoded protein (isoform 3) is shorter and has a distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATTCTG ACTCCGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTTCC CAAAATGAAG 
ATTCGAAGAA GGACGTCCAA ACAAGATCGC TCCTTCGATA TCCTAAAAAA ATCCAAGCCG 
CCCTCGACAT TGCTTGCTGC AGGCCGGCTT TCAGACATGC TGAATGGAGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGATCAGGTT GGTGATTTGG ATATCAGCTA TATTAATATA 
GAGGGAATCA CTGCCACTAC CAGCCCTGAA TCCAGAGGTT GCACTCTGTG GCCTCAGAGC 
AGCAAACACA CCCTTCCTAC AGAAACCAGT CCCAGTGTGT ACCCACTTAG TGAAAATGTC 
GAAGGGACAG CACACACTGA AGCCCAGCAG TCCTTCATGT CACCATCAAG TTCGTGTGCT 
TCCAACTTGA ATCTTTCTTT TGGTTGGCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AAAAGAAGTT CTAGCCTTGA TGCCTTGGAC GCCGACAGTG AAGGGGAAGG GCATTCTGAG 
CCATCCCACA TCTGTTACAC TCCAGGGTCT CAGAGCTCCT CAAGAACTGG GATTCCTAGT 
GGGGATGAAT TGGACTCTTT TGAGACTAAC ACTGAACCGG ATTTTAATAT CTCCAGGGCT 
GAATCCCTTC CTCTATCAAG TAATCTACAG TCGAAGGAAT CACTGCTTTC TGGAGTTCGC 
TCACGTTCTT ATTCTTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG TTTGGTGCGT 
GAATTAACAG TATGCAGTTC AAGTGAAGAG CAAAGAGCTT ACAGCTTATC GGAGCCACCA 
AGAGAAAACA GAATTCAGGA AGAAGAATGG GATAAATACA TCATACCTGC CAAATCAGAG 
TCTGAAAAAT ATAAAGTGAG TCGAACTTTC AGTTTCCTCA TGAATAGGAT GACTAGCCCT 
CGGAATAAAT CAAAGACAAA AAGCAAGGAT GCCAAAGATA AAGAGAAGCT GAATCGACAT 
CAGTTTGCCC CAGGAACATT CTCTGGGGTT CTGCAGTGTT TGGTTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTGCA GTGTTCTAAC TGTAATGCAA ATGTGCACAA AGGTTGTAAA 
GATGCTGCGC CTGCATGCAC CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTTAGAGAC ATCCCACAGC CTGGTCTCTC CTTGCACCCT 
TCTTCCTCCG TGCCTGTTGG ATTGCCGACT GGAAGGAGGG AGACTGTGGG ACAGGTCCAT 
CCATTGTCCA GAAGTGTTCC AGGCACCACC TTGGAAAGCT TCAGGAGGTC AGCCACATCC 
TTGGAGTCTG AGAGTGACCA TAACAGCTGC AGAAGCAGGT CTCATTCTGA TGAGCTGCTA 
CAGTCCATGG GCTCTTCTCC CTCTACAGAG TCTTTCATAA TGGAAGATGT TGTGGATTCT 
TCTCTGTGGA GTGACCTCAG CAGTGATGCC CAGGAGTTTG AAGCAGAATC TTGGAGTCTT 
GTGGTGGATC CCTCATTTTG TAATAGGCAG GAGAAGGATG TCATCAAAAG ACAGGATGTC 
ATTTTTGAGC TAATGCAAAC AGAGATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGCATGAA AGAGGAGCTG CAGCTGGACC ACAGCACCGT GGATAAAATT 
TTCCCCTGTT TAGATGAGTT GCTTGAAATC CACAGGCATT TCTTCTACAG TATGAAGGAA 
CGAAGGCAGG AATCCTGTGC TGGCAGCGAC AGGAATTTTG TGATCGACCG AATTGGAGAT 
ATTTTGGTAC AACAGTTTTC AGAAGAAAAT GCAAGTAAAA TGAAGAAAAT ATATGGAGAA 
TTCTGTTGCC ATCATAAAGA AGCTGTTAAC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTTCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGTTGGTCAC TCAGCGTATT ACAAAATACC CTGTCTTGGT GGAAAGGATA 
TTGCAGTACA CAAAGGAAAG AACTGAGGAA CATAAAGACT TACGCAAAGC GCTTTGCTTA 
ATTAAAGACA TGATTGCAAC AGTGGATTTA AAAGTCAATG AATATGAGAA AAACCAAAAA 
TGGCTTGAGA TCCTAAATAA GATTGAAAAC AAAACATACA CGAAGCTCAA AAATGGACAT 
GTGTTTAGGA AGCAGGCACT GATGAGTGAA GAAAGGACTC TGTTATATGA TGGCCTTGTT 
TACTGGAAAA CTGCTACAGG TCGTTTCAAA GATATCCTAG CTCTACTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCCGTTGA TCAGAAGCCA 
TCAGTTATTT CCCTTCAAAA GCTTATTGCT AGAGAAGTTG CTAATGAGGA GAGAGGAATG 
TTTCTGATCA GTGCTTCATC TGCTGGTCCT GAGATGTATG AAATTCACAC CAATTCCAAG 
GAGGAACGCA ATAACTGGAT GAGACGGATC CAGCAGGCTG TAGAAAGTTG TCCTGAAGAA 
AAAGGGGGAA GGACAAGTGA ATCTGATGAA GACAAGAGGA AAGCTGAAGC CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCACT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATCTA TGCTGAACTT GGAGAACTGA GCGGATTTGA GGACGTCCAT 
CTAGAGCCCC ACCTCCTTAT TAAACCTGAC CCAGGCGAGC CTCCCCAGGC AGCCTCATTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTACAAGTTG CAGTGAAGGC CTCACAGATG 
GGCGCCGTGA GTCAATCATG TGAGGACAGT TGTGGAGACT CTGTCTTGGC GGACACACTC 
AGTTCTCATG ATGTACCAGG ATCACCGACT GCCTCATTAG TCACAGGAGG GAGAGAAGGA 
AGAGGCTGTT CGGATGTGGA TCCCGGGATC CAGGGTGTGG TAACCGACTT GGCCGTCTCT 
GATGCAGGGG AGAAGGTGGA ATGTAGAAAT TTTCCAGGTT CTTCACAATC AGAGATTATA 
CAAGCCATAC AGAATTTAAC CCGTCTCTTA TACAGCCTTC AGGCCGCCTT GACCATTCAG 
GACAGCCACA TTGAGATCCA CAGGCTGGTT CTCCAGCAGC AGGAGGGCCT GTCTCTCGGC 
CACTCTATCC TCCGAGGCGG CCCCTTGCAG GACCAGAAGT CTCGCGACGC GGACAGGCAG 
CATGAGGAGC TGGCCAATGT GCACCAGCTT CAGCACCAGC TCCAGCAGGA GCAGCGGCGC 
TGGCTGCGCA GGTGTGAGCA GCAGCAGCGG GCGCAGGCGA CCAGGGAGAG CTGGCTGCAG 
GAGCGGGAGC GGGAGTGCCA GTCGCAGGAG GAGCTGCTGC TGCGGAGCCG GGGCGAGCTG 
GACCTCCAGC TCCAGGAGTA CCAGCACAGC CTGGAGCGGC TGAGGGAGGG CCAGCGCCTG 
GTGGAGAGGG AGCAGGCGAG GATGCGGGCC CAGCAGAGCC TGCTGGGCCA CTGGAAGCAC 
GGCCGGCAGA GGAGCCTGCC CGCGGTGCTC CTTCCGGGTG GCCCCGAGGT AATGGAACTT 
AATCGATCTG AGAGTTTATG TCATGAAAAC TCATTCTTCA TCAATGAAGC TTTAGTACAA 
ATGTCATTTA ACACTTTCAA CAAACTGAAT CCATCAGTTA TCCATCAGGA TGCCACTTAC 
CCTACAACTC AATCTCATTC TGACTTGGTG AGGACTAGTG AACATCAAGT AGACCTCAAG 
GTGGACCCTT CTCAGCCTTC GAATGTCAGT CACAAACTGT GGACAGCCGC TGGTTCCGGC 
CATCAGATAC TTCCTTTCCA TGAAAGCAGC AAGGATTCTT GTAAAAATGA TTTGGACACC 
TCCCACACTG AGTCCCCAAC CCCCCATGAC TCAAATTCAC ACCGCCCTCA ACTGCAGGCG 
TTTATAACAG AAGCAAAGCT AAATCTACCG ACAAGGACAA TGACCAGACA AGATGGGGAA 
ACTGGAGATG GAGCCAAAGA AAATATTGTT TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001231293.1
CDS	72..4250
Translation	MDSDSDSPFNYSWPSFPKMKIRRRTSKQDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	NM_001244364.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001244364.1

ORF Insert Sequence:

ATGGATTCTG ACTCCGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTTCC CAAAATGAAG 
ATTCGAAGAA GGACGTCCAA ACAAGATCGC TCCTTCGATA TCCTAAAAAA ATCCAAGCCG 
CCCTCGACAT TGCTTGCTGC AGGCCGGCTT TCAGACATGC TGAATGGAGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGATCAGGTT GGTGATTTGG ATATCAGCTA TATTAATATA 
GAGGGAATCA CTGCCACTAC CAGCCCTGAA TCCAGAGGTT GCACTCTGTG GCCTCAGAGC 
AGCAAACACA CCCTTCCTAC AGAAACCAGT CCCAGTGTGT ACCCACTTAG TGAAAATGTC 
GAAGGGACAG CACACACTGA AGCCCAGCAG TCCTTCATGT CACCATCAAG TTCGTGTGCT 
TCCAACTTGA ATCTTTCTTT TGGTTGGCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AAAAGAAGTT CTAGCCTTGA TGCCTTGGAC GCCGACAGTG AAGGGGAAGG GCATTCTGAG 
CCATCCCACA TCTGTTACAC TCCAGGGTCT CAGAGCTCCT CAAGAACTGG GATTCCTAGT 
GGGGATGAAT TGGACTCTTT TGAGACTAAC ACTGAACCGG ATTTTAATAT CTCCAGGGCT 
GAATCCCTTC CTCTATCAAG TAATCTACAG TCGAAGGAAT CACTGCTTTC TGGAGTTCGC 
TCACGTTCTT ATTCTTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG TTTGGTGCGT 
GAATTAACAG TATGCAGTTC AAGTGAAGAG CAAAGAGCTT ACAGCTTATC GGAGCCACCA 
AGAGAAAACA GAATTCAGGA AGAAGAATGG GATAAATACA TCATACCTGC CAAATCAGAG 
TCTGAAAAAT ATAAAGTGAG TCGAACTTTC AGTTTCCTCA TGAATAGGAT GACTAGCCCT 
CGGAATAAAT CAAAGACAAA AAGCAAGGAT GCCAAAGATA AAGAGAAGCT GAATCGACAT 
CAGTTTGCCC CAGGAACATT CTCTGGGGTT CTGCAGTGTT TGGTTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTGCA GTGTTCTAAC TGTAATGCAA ATGTGCACAA AGGTTGTAAA 
GATGCTGCGC CTGCATGCAC CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTTAGAGAC ATCCCACAGC CTGGTCTCTC CTTGCACCCT 
TCTTCCTCCG TGCCTGTTGG ATTGCCGACT GGAAGGAGGG AGACTGTGGG ACAGGTCCAT 
CCATTGTCCA GAAGTGTTCC AGGCACCACC TTGGAAAGCT TCAGGAGGTC AGCCACATCC 
TTGGAGTCTG AGAGTGACCA TAACAGCTGC AGAAGCAGGT CTCATTCTGA TGAGCTGCTA 
CAGTCCATGG GCTCTTCTCC CTCTACAGAG TCTTTCATAA TGGAAGATGT TGTGGATTCT 
TCTCTGTGGA GTGACCTCAG CAGTGATGCC CAGGAGTTTG AAGCAGAATC TTGGAGTCTT 
GTGGTGGATC CCTCATTTTG TAATAGGCAG GAGAAGGATG TCATCAAAAG ACAGGATGTC 
ATTTTTGAGC TAATGCAAAC AGAGATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGCATGAA AGAGGAGCTG CAGCTGGACC ACAGCACCGT GGATAAAATT 
TTCCCCTGTT TAGATGAGTT GCTTGAAATC CACAGGCATT TCTTCTACAG TATGAAGGAA 
CGAAGGCAGG AATCCTGTGC TGGCAGCGAC AGGAATTTTG TGATCGACCG AATTGGAGAT 
ATTTTGGTAC AACAGTTTTC AGAAGAAAAT GCAAGTAAAA TGAAGAAAAT ATATGGAGAA 
TTCTGTTGCC ATCATAAAGA AGCTGTTAAC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTTCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGTTGGTCAC TCAGCGTATT ACAAAATACC CTGTCTTGGT GGAAAGGATA 
TTGCAGTACA CAAAGGAAAG AACTGAGGAA CATAAAGACT TACGCAAAGC GCTTTGCTTA 
ATTAAAGACA TGATTGCAAC AGTGGATTTA AAAGTCAATG AATATGAGAA AAACCAAAAA 
TGGCTTGAGA TCCTAAATAA GATTGAAAAC AAAACATACA CGAAGCTCAA AAATGGACAT 
GTGTTTAGGA AGCAGGCACT GATGAGTGAA GAAAGGACTC TGTTATATGA TGGCCTTGTT 
TACTGGAAAA CTGCTACAGG TCGTTTCAAA GATATCCTAG CTCTACTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCCGTTGA TCAGAAGCCA 
TCAGTTATTT CCCTTCAAAA GCTTATTGCT AGAGAAGTTG CTAATGAGGA GAGAGGAATG 
TTTCTGATCA GTGCTTCATC TGCTGGTCCT GAGATGTATG AAATTCACAC CAATTCCAAG 
GAGGAACGCA ATAACTGGAT GAGACGGATC CAGCAGGCTG TAGAAAGTTG TCCTGAAGAA 
AAAGGGGGAA GGACAAGTGA ATCTGATGAA GACAAGAGGA AAGCTGAAGC CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCACT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATCTA TGCTGAACTT GGAGAACTGA GCGGATTTGA GGACGTCCAT 
CTAGAGCCCC ACCTCCTTAT TAAACCTGAC CCAGGCGAGC CTCCCCAGGC AGCCTCATTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTACAAGTTG CAGTGAAGGC CTCACAGATG 
GGCGCCGTGA GTCAATCATG TGAGGACAGT TGTGGAGACT CTGTCTTGGC GGACACACTC 
AGTTCTCATG ATGTACCAGG ATCACCGACT GCCTCATTAG TCACAGGAGG GAGAGAAGGA 
AGAGGCTGTT CGGATGTGGA TCCCGGGATC CAGGGTGTGG TAACCGACTT GGCCGTCTCT 
GATGCAGGGG AGAAGGTGGA ATGTAGAAAT TTTCCAGGTT CTTCACAATC AGAGATTATA 
CAAGCCATAC AGAATTTAAC CCGTCTCTTA TACAGCCTTC AGGCCGCCTT GACCATTCAG 
GACAGCCACA TTGAGATCCA CAGGCTGGTT CTCCAGCAGC AGGAGGGCCT GTCTCTCGGC 
CACTCTATCC TCCGAGGCGG CCCCTTGCAG GACCAGAAGT CTCGCGACGC GGACAGGCAG 
CATGAGGAGC TGGCCAATGT GCACCAGCTT CAGCACCAGC TCCAGCAGGA GCAGCGGCGC 
TGGCTGCGCA GGTGTGAGCA GCAGCAGCGG GCGCAGGCGA CCAGGGAGAG CTGGCTGCAG 
GAGCGGGAGC GGGAGTGCCA GTCGCAGGAG GAGCTGCTGC TGCGGAGCCG GGGCGAGCTG 
GACCTCCAGC TCCAGGAGTA CCAGCACAGC CTGGAGCGGC TGAGGGAGGG CCAGCGCCTG 
GTGGAGAGGG AGCAGGCGAG GATGCGGGCC CAGCAGAGCC TGCTGGGCCA CTGGAAGCAC 
GGCCGGCAGA GGAGCCTGCC CGCGGTGCTC CTTCCGGGTG GCCCCGAGGT AATGGAACTT 
AATCGATCTG AGAGTTTATG TCATGAAAAC TCATTCTTCA TCAATGAAGC TTTAGTACAA 
ATGTCATTTA ACACTTTCAA CAAACTGAAT CCATCAGTTA TCCATCAGGA TGCCACTTAC 
CCTACAACTC AATCTCATTC TGACTTGGTG AGGACTAGTG AACATCAAGT AGACCTCAAG 
GTGGACCCTT CTCAGCCTTC GAATGTCAGT CACAAACTGT GGACAGCCGC TGGTTCCGGC 
CATCAGATAC TTCCTTTCCA TGAAAGCAGC AAGGATTCTT GTAAAAATGA TTTGGACACC 
TCCCACACTG AGTCCCCAAC CCCCCATGAC TCAAATTCAC ACCGCCCTCA ACTGCAGGCG 
TTTATAACAG AAGCAAAGCT AAATCTACCG ACAAGGACAA TGACCAGACA AGATGGGGAA 
ACTGGAGATG GAGCCAAAGA AAATATTGTT TACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( NM_001080479.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001073948.2 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 1, mRNA.

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CloneID	OHu21533
Clone ID Related Accession (Same CDS sequence)	XM_011543546.1 , NM_001080479.2 , XM_011543546.2
Accession Version	NM_001080479.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK094713.1, BC157846.1, AC091868.3, AC093283.3 and CN478799.1. This sequence is a reference standard in the RefSeqGene project. On Apr 25, 2010 this sequence version replaced NM_001080479.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC157846.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGGC TCCAGTATGA CAAAGTGCAG TTGTACGTTG 
ACATCTCCCC CGGGACTGTG GACTGGAACC ACATCTACTT TGAAAGATTT GGACACCTCC 
CACACTGAGT CCCCAACCCC CCATGACTCA AATTCACACC GCCCTCAACT GCAGGCGTTT 
ATAACAGAAG CAAAGCTAAA TCTACCGACA AGGACAATGA CCAGACAAGA TGGGGAAACT 
GGAGATGGAG CCAAAGAAAA TATTGTTTAC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001073948.2
CDS	177..5372
Translation	MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	NM_001080479.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080479.2

ORF Insert Sequence:

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGGC TCCAGTATGA CAAAGTGCAG TTGTACGTTG 
ACATCTCCCC CGGGACTGTG GACTGGAACC ACATCTACTT TGAAAGATTT GGACACCTCC 
CACACTGAGT CCCCAACCCC CCATGACTCA AATTCACACC GCCCTCAACT GCAGGCGTTT 
ATAACAGAAG CAAAGCTAAA TCTACCGACA AGGACAATGA CCAGACAAGA TGGGGAAACT 
GGAGATGGAG CCAAAGAAAA TATTGTTTAC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_011543546.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541848.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

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CloneID	OHu21533
Clone ID Related Accession (Same CDS sequence)	XM_011543546.1 , NM_001080479.2 , XM_011543546.2
Accession Version	XM_011543546.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGGC TCCAGTATGA CAAAGTGCAG TTGTACGTTG 
ACATCTCCCC CGGGACTGTG GACTGGAACC ACATCTACTT TGAAAGATTT GGACACCTCC 
CACACTGAGT CCCCAACCCC CCATGACTCA AATTCACACC GCCCTCAACT GCAGGCGTTT 
ATAACAGAAG CAAAGCTAAA TCTACCGACA AGGACAATGA CCAGACAAGA TGGGGAAACT 
GGAGATGGAG CCAAAGAAAA TATTGTTTAC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541848.1
CDS	184..5379
Translation	MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	XM_011543546.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543546.1

ORF Insert Sequence:

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGGC TCCAGTATGA CAAAGTGCAG TTGTACGTTG 
ACATCTCCCC CGGGACTGTG GACTGGAACC ACATCTACTT TGAAAGATTT GGACACCTCC 
CACACTGAGT CCCCAACCCC CCATGACTCA AATTCACACC GCCCTCAACT GCAGGCGTTT 
ATAACAGAAG CAAAGCTAAA TCTACCGACA AGGACAATGA CCAGACAAGA TGGGGAAACT 
GGAGATGGAG CCAAAGAAAA TATTGTTTAC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_011543546.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541848.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

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CloneID	OHu21533
Clone ID Related Accession (Same CDS sequence)	XM_011543546.1 , NM_001080479.2 , XM_011543546.2
Accession Version	XM_011543546.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5196bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543546.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGGC TCCAGTATGA CAAAGTGCAG TTGTACGTTG 
ACATCTCCCC CGGGACTGTG GACTGGAACC ACATCTACTT TGAAAGATTT GGACACCTCC 
CACACTGAGT CCCCAACCCC CCATGACTCA AATTCACACC GCCCTCAACT GCAGGCGTTT 
ATAACAGAAG CAAAGCTAAA TCTACCGACA AGGACAATGA CCAGACAAGA TGGGGAAACT 
GGAGATGGAG CCAAAGAAAA TATTGTTTAC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541848.1
CDS	179..5374
Translation	MELSCSEAPLYGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	XM_011543546.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543546.2

ORF Insert Sequence:

ATGGAGTTGA GCTGCAGCGA AGCACCTCTT TACGGGCAGA TGATGATCTA TGCGAAGTTT 
GACAAAAATG TGTATCTTCC TGAAGATGCT GAGTTTTACT TTACTTATGA CGGATCTCAT 
CAGCGACATG TCATGATTGC AGAGCGCATC GAGGATAACG TTCTCCAGTC CAGCGTCCCA 
GGCCATGGGC TTCAGGAGAC GGTGACGGTA TCTGTGTGCC TCTGCTCGGA AGGTTACTCT 
CCGGTGACCA TGGGCTCTGG CTCAGTGACC TACGTGGACA ACATGGCTTG CAGGCTGGCT 
CGTCTGCTGG TGACGCAGGC CAATCGCCTC ACAGCCTGCA GCCACCAGAC CCTGCTGACC 
CCATTTGCCT TGACGGCAGG AGCACTGCCT GCCTTGGATG AGGAGCTCGT GCTGGCTCTG 
ACCCATCTGG AATTGCCTCT AGAGTGGACT GTGTTGGGAA GTTCTTCACT TGAAGTATCT 
TCTCACAGAG AATCTCTTCT ACACCTGGCT ATGAGATGGG GCCTGGCTAA ACTTTCCCAG 
TTCTTCTTGT GTCTCCCGGG GGGAGTCCAG GCCTTGGCTT TACCCAACGA AGAGGGTGCC 
ACACCATTAG ACTTAGCTTT ACGTGAAGGA CACTCCAAGC TGGTGGAAGA CGTCACAAAT 
TTTCAGGGCA GATGGTCCCC AAGCTTCTCC CGAGTGCAGC TCAGTGAAGA AGCCTCCTTG 
CATTACATTC ACTCATCGGA AACGCTGACC CTGACCCTGA ACCACACAGC CGAGCATTTG 
TTGGAGGCAG ATATTAAACT CTTCCGGAAA TACTTTTGGG ATAGAGCCTT TCTTGTCAAG 
GCCTTTGAGC CAGAAGCCAG GCCAGAGGAA AGAACAGCTA TGCCCTCCAG CGGTGCAGAA 
ACTGAAGAAG AGATTAAGAA TTCAGTGTCC AGCAGATCAG CAGCTGAAAA GGAAGATATA 
AAGCGTGTCA AAAGCCTGGT GGTTCAACAC AATGAACATG AAGACCAGCA CAGCCTAGAT 
TTGGATCGCT CCTTCGATAT CCTAAAAAAA TCCAAGCCGC CCTCGACATT GCTTGCTGCA 
GGCCGGCTTT CAGACATGCT GAATGGAGGT GATGAAGTCT ACGCTAACTG TATGGTGATT 
GATCAGGTTG GTGATTTGGA TATCAGCTAT ATTAATATAG AGGGAATCAC TGCCACTACC 
AGCCCTGAAT CCAGAGGTTG CACTCTGTGG CCTCAGAGCA GCAAACACAC CCTTCCTACA 
GAAACCAGTC CCAGTGTGTA CCCACTTAGT GAAAATGTCG AAGGGACAGC ACACACTGAA 
GCCCAGCAGT CCTTCATGTC ACCATCAAGT TCGTGTGCTT CCAACTTGAA TCTTTCTTTT 
GGTTGGCATG GATTTGAAAA GGAACAAAGT CATCTAAAGA AAAGAAGTTC TAGCCTTGAT 
GCCTTGGACG CCGACAGTGA AGGGGAAGGG CATTCTGAGC CATCCCACAT CTGTTACACT 
CCAGGGTCTC AGAGCTCCTC AAGAACTGGG ATTCCTAGTG GGGATGAATT GGACTCTTTT 
GAGACTAACA CTGAACCGGA TTTTAATATC TCCAGGGCTG AATCCCTTCC TCTATCAAGT 
AATCTACAGT CGAAGGAATC ACTGCTTTCT GGAGTTCGCT CACGTTCTTA TTCTTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGT TTGGTGCGTG AATTAACAGT ATGCAGTTCA 
AGTGAAGAGC AAAGAGCTTA CAGCTTATCG GAGCCACCAA GAGAAAACAG AATTCAGGAA 
GAAGAATGGG ATAAATACAT CATACCTGCC AAATCAGAGT CTGAAAAATA TAAAGTGAGT 
CGAACTTTCA GTTTCCTCAT GAATAGGATG ACTAGCCCTC GGAATAAATC AAAGACAAAA 
AGCAAGGATG CCAAAGATAA AGAGAAGCTG AATCGACATC AGTTTGCCCC AGGAACATTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGTGAT AAAACACTCC TGGGGAAAGA GTCACTGCAG 
TGTTCTAACT GTAATGCAAA TGTGCACAAA GGTTGTAAAG ATGCTGCGCC TGCATGCACC 
AAGAAATTCC AAGAGAAATA TAACAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCA 
TTTAGAGACA TCCCACAGCC TGGTCTCTCC TTGCACCCTT CTTCCTCCGT GCCTGTTGGA 
TTGCCGACTG GAAGGAGGGA GACTGTGGGA CAGGTCCATC CATTGTCCAG AAGTGTTCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGTCA GCCACATCCT TGGAGTCTGA GAGTGACCAT 
AACAGCTGCA GAAGCAGGTC TCATTCTGAT GAGCTGCTAC AGTCCATGGG CTCTTCTCCC 
TCTACAGAGT CTTTCATAAT GGAAGATGTT GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
AGTGATGCCC AGGAGTTTGA AGCAGAATCT TGGAGTCTTG TGGTGGATCC CTCATTTTGT 
AATAGGCAGG AGAAGGATGT CATCAAAAGA CAGGATGTCA TTTTTGAGCT AATGCAAACA 
GAGATGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGCATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACCGTG GATAAAATTT TCCCCTGTTT AGATGAGTTG 
CTTGAAATCC ACAGGCATTT CTTCTACAGT ATGAAGGAAC GAAGGCAGGA ATCCTGTGCT 
GGCAGCGACA GGAATTTTGT GATCGACCGA ATTGGAGATA TTTTGGTACA ACAGTTTTCA 
GAAGAAAATG CAAGTAAAAT GAAGAAAATA TATGGAGAAT TCTGTTGCCA TCATAAAGAA 
GCTGTTAACC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ATCTTTTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GTTGGTCACT 
CAGCGTATTA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAAAGA 
ACTGAGGAAC ATAAAGACTT ACGCAAAGCG CTTTGCTTAA TTAAAGACAT GATTGCAACA 
GTGGATTTAA AAGTCAATGA ATATGAGAAA AACCAAAAAT GGCTTGAGAT CCTAAATAAG 
ATTGAAAACA AAACATACAC GAAGCTCAAA AATGGACATG TGTTTAGGAA GCAGGCACTG 
ATGAGTGAAG AAAGGACTCT GTTATATGAT GGCCTTGTTT ACTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCCGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAAAAG 
CTTATTGCTA GAGAAGTTGC TAATGAGGAG AGAGGAATGT TTCTGATCAG TGCTTCATCT 
GCTGGTCCTG AGATGTATGA AATTCACACC AATTCCAAGG AGGAACGCAA TAACTGGATG 
AGACGGATCC AGCAGGCTGT AGAAAGTTGT CCTGAAGAAA AAGGGGGAAG GACAAGTGAA 
TCTGATGAAG ACAAGAGGAA AGCTGAAGCC AGAGTGGCCA AAATTCAGCA ATGTCAAGAA 
ATACTCACTA ACCAAGACCA ACAAATTTGT GCGTATTTGG AGGAGAAGCT GCATATCTAT 
GCTGAACTTG GAGAACTGAG CGGATTTGAG GACGTCCATC TAGAGCCCCA CCTCCTTATT 
AAACCTGACC CAGGCGAGCC TCCCCAGGCA GCCTCATTAC TGGCAGCAGC ACTGAAAGAA 
GCTGAGAGCC TACAAGTTGC AGTGAAGGCC TCACAGATGG GCGCCGTGAG TCAATCATGT 
GAGGACAGTT GTGGAGACTC TGTCTTGGCG GACACACTCA GTTCTCATGA TGTACCAGGA 
TCACCGACTG CCTCATTAGT CACAGGAGGG AGAGAAGGAA GAGGCTGTTC GGATGTGGAT 
CCCGGGATCC AGGGTGTGGT AACCGACTTG GCCGTCTCTG ATGCAGGGGA GAAGGTGGAA 
TGTAGAAATT TTCCAGGTTC TTCACAATCA GAGATTATAC AAGCCATACA GAATTTAACC 
CGTCTCTTAT ACAGCCTTCA GGCCGCCTTG ACCATTCAGG ACAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAGCAGCA GGAGGGCCTG TCTCTCGGCC ACTCTATCCT CCGAGGCGGC 
CCCTTGCAGG ACCAGAAGTC TCGCGACGCG GACAGGCAGC ATGAGGAGCT GGCCAATGTG 
CACCAGCTTC AGCACCAGCT CCAGCAGGAG CAGCGGCGCT GGCTGCGCAG GTGTGAGCAG 
CAGCAGCGGG CGCAGGCGAC CAGGGAGAGC TGGCTGCAGG AGCGGGAGCG GGAGTGCCAG 
TCGCAGGAGG AGCTGCTGCT GCGGAGCCGG GGCGAGCTGG ACCTCCAGCT CCAGGAGTAC 
CAGCACAGCC TGGAGCGGCT GAGGGAGGGC CAGCGCCTGG TGGAGAGGGA GCAGGCGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGGGCCAC TGGAAGCACG GCCGGCAGAG GAGCCTGCCC 
GCGGTGCTCC TTCCGGGTGG CCCCGAGGTA ATGGAACTTA ATCGATCTGA GAGTTTATGT 
CATGAAAACT CATTCTTCAT CAATGAAGCT TTAGTACAAA TGTCATTTAA CACTTTCAAC 
AAACTGAATC CATCAGTTAT CCATCAGGAT GCCACTTACC CTACAACTCA ATCTCATTCT 
GACTTGGTGA GGACTAGTGA ACATCAAGTA GACCTCAAGG TGGACCCTTC TCAGCCTTCG 
AATGTCAGTC ACAAACTGTG GACAGCCGCT GGTTCCGGCC ATCAGATACT TCCTTTCCAT 
GAAAGCAGCA AGGATTCTTG TAAAAATGGC TCCAGTATGA CAAAGTGCAG TTGTACGTTG 
ACATCTCCCC CGGGACTGTG GACTGGAACC ACATCTACTT TGAAAGATTT GGACACCTCC 
CACACTGAGT CCCCAACCCC CCATGACTCA AATTCACACC GCCCTCAACT GCAGGCGTTT 
ATAACAGAAG CAAAGCTAAA TCTACCGACA AGGACAATGA CCAGACAAGA TGGGGAAACT 
GGAGATGGAG CCAAAGAAAA TATTGTTTAC CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_005248568.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005248625.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

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CloneID	OHu39668
Clone ID Related Accession (Same CDS sequence)	XM_005248568.4
Accession Version	XM_005248568.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005248568.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCTG ACTCCGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTTCC CAAAATGAAG 
ATTCGAAGAA GGACGTCCAA ACAAGATCGC TCCTTCGATA TCCTAAAAAA ATCCAAGCCG 
CCCTCGACAT TGCTTGCTGC AGGCCGGCTT TCAGACATGC TGAATGGAGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGATCAGGTT GGTGATTTGG ATATCAGCTA TATTAATATA 
GAGGGAATCA CTGCCACTAC CAGCCCTGAA TCCAGAGGTT GCACTCTGTG GCCTCAGAGC 
AGCAAACACA CCCTTCCTAC AGAAACCAGT CCCAGTGTGT ACCCACTTAG TGAAAATGTC 
GAAGGGACAG CACACACTGA AGCCCAGCAG TCCTTCATGT CACCATCAAG TTCGTGTGCT 
TCCAACTTGA ATCTTTCTTT TGGTTGGCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AAAAGAAGTT CTAGCCTTGA TGCCTTGGAC GCCGACAGTG AAGGGGAAGG GCATTCTGAG 
CCATCCCACA TCTGTTACAC TCCAGGGTCT CAGAGCTCCT CAAGAACTGG GATTCCTAGT 
GGGGATGAAT TGGACTCTTT TGAGACTAAC ACTGAACCGG ATTTTAATAT CTCCAGGGCT 
GAATCCCTTC CTCTATCAAG TAATCTACAG TCGAAGGAAT CACTGCTTTC TGGAGTTCGC 
TCACGTTCTT ATTCTTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG TTTGGTGCGT 
GAATTAACAG TATGCAGTTC AAGTGAAGAG CAAAGAGCTT ACAGCTTATC GGAGCCACCA 
AGAGAAAACA GAATTCAGGA AGAAGAATGG GATAAATACA TCATACCTGC CAAATCAGAG 
TCTGAAAAAT ATAAAGTGAG TCGAACTTTC AGTTTCCTCA TGAATAGGAT GACTAGCCCT 
CGGAATAAAT CAAAGACAAA AAGCAAGGAT GCCAAAGATA AAGAGAAGCT GAATCGACAT 
CAGTTTGCCC CAGGAACATT CTCTGGGGTT CTGCAGTGTT TGGTTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTGCA GTGTTCTAAC TGTAATGCAA ATGTGCACAA AGGTTGTAAA 
GATGCTGCGC CTGCATGCAC CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTTAGAGAC ATCCCACAGC CTGGTCTCTC CTTGCACCCT 
TCTTCCTCCG TGCCTGTTGG ATTGCCGACT GGAAGGAGGG AGACTGTGGG ACAGGTCCAT 
CCATTGTCCA GAAGTGTTCC AGGCACCACC TTGGAAAGCT TCAGGAGGTC AGCCACATCC 
TTGGAGTCTG AGAGTGACCA TAACAGCTGC AGAAGCAGGT CTCATTCTGA TGAGCTGCTA 
CAGTCCATGG GCTCTTCTCC CTCTACAGAG TCTTTCATAA TGGAAGATGT TGTGGATTCT 
TCTCTGTGGA GTGACCTCAG CAGTGATGCC CAGGAGTTTG AAGCAGAATC TTGGAGTCTT 
GTGGTGGATC CCTCATTTTG TAATAGGCAG GAGAAGGATG TCATCAAAAG ACAGGATGTC 
ATTTTTGAGC TAATGCAAAC AGAGATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGCATGAA AGAGGAGCTG CAGCTGGACC ACAGCACCGT GGATAAAATT 
TTCCCCTGTT TAGATGAGTT GCTTGAAATC CACAGGCATT TCTTCTACAG TATGAAGGAA 
CGAAGGCAGG AATCCTGTGC TGGCAGCGAC AGGAATTTTG TGATCGACCG AATTGGAGAT 
ATTTTGGTAC AACAGTTTTC AGAAGAAAAT GCAAGTAAAA TGAAGAAAAT ATATGGAGAA 
TTCTGTTGCC ATCATAAAGA AGCTGTTAAC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTTCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGTTGGTCAC TCAGCGTATT ACAAAATACC CTGTCTTGGT GGAAAGGATA 
TTGCAGTACA CAAAGGAAAG AACTGAGGAA CATAAAGACT TACGCAAAGC GCTTTGCTTA 
ATTAAAGACA TGATTGCAAC AGTGGATTTA AAAGTCAATG AATATGAGAA AAACCAAAAA 
TGGCTTGAGA TCCTAAATAA GATTGAAAAC AAAACATACA CGAAGCTCAA AAATGGACAT 
GTGTTTAGGA AGCAGGCACT GATGAGTGAA GAAAGGACTC TGTTATATGA TGGCCTTGTT 
TACTGGAAAA CTGCTACAGG TCGTTTCAAA GATATCCTAG CTCTACTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCCGTTGA TCAGAAGCCA 
TCAGTTATTT CCCTTCAAAA GCTTATTGCT AGAGAAGTTG CTAATGAGGA GAGAGGAATG 
TTTCTGATCA GTGCTTCATC TGCTGGTCCT GAGATGTATG AAATTCACAC CAATTCCAAG 
GAGGAACGCA ATAACTGGAT GAGACGGATC CAGCAGGCTG TAGAAAGTTG TCCTGAAGAA 
AAAGGGGGAA GGACAAGTGA ATCTGATGAA GACAAGAGGA AAGCTGAAGC CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCACT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATCTA TGCTGAACTT GGAGAACTGA GCGGATTTGA GGACGTCCAT 
CTAGAGCCCC ACCTCCTTAT TAAACCTGAC CCAGGCGAGC CTCCCCAGGC AGCCTCATTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTACAAGTTG CAGTGAAGGC CTCACAGATG 
GGCGCCGTGA GTCAATCATG TGAGGACAGT TGTGGAGACT CTGTCTTGGC GGACACACTC 
AGTTCTCATG ATGTACCAGG ATCACCGACT GCCTCATTAG TCACAGGAGG GAGAGAAGGA 
AGAGGCTGTT CGGATGTGGA TCCCGGGATC CAGGGTGTGG TAACCGACTT GGCCGTCTCT 
GATGCAGGGG AGAAGGTGGA ATGTAGAAAT TTTCCAGGTT CTTCACAATC AGAGATTATA 
CAAGCCATAC AGAATTTAAC CCGTCTCTTA TACAGCCTTC AGGCCGCCTT GACCATTCAG 
GACAGCCACA TTGAGATCCA CAGGCTGGTT CTCCAGCAGC AGGAGGGCCT GTCTCTCGGC 
CACTCTATCC TCCGAGGCGG CCCCTTGCAG GACCAGAAGT CTCGCGACGC GGACAGGCAG 
CATGAGGAGC TGGCCAATGT GCACCAGCTT CAGCACCAGC TCCAGCAGGA GCAGCGGCGC 
TGGCTGCGCA GGTGTGAGCA GCAGCAGCGG GCGCAGGCGA CCAGGGAGAG CTGGCTGCAG 
GAGCGGGAGC GGGAGTGCCA GTCGCAGGAG GAGCTGCTGC TGCGGAGCCG GGGCGAGCTG 
GACCTCCAGC TCCAGGAGTA CCAGCACAGC CTGGAGCGGC TGAGGGAGGG CCAGCGCCTG 
GTGGAGAGGG AGCAGGCGAG GATGCGGGCC CAGCAGAGCC TGCTGGGCCA CTGGAAGCAC 
GGCCGGCAGA GGAGCCTGCC CGCGGTGCTC CTTCCGGGTG GCCCCGAGGT AATGGAACTT 
AATCGATCTG AGAGTTTATG TCATGAAAAC TCATTCTTCA TCAATGAAGC TTTAGTACAA 
ATGTCATTTA ACACTTTCAA CAAACTGAAT CCATCAGTTA TCCATCAGGA TGCCACTTAC 
CCTACAACTC AATCTCATTC TGACTTGGTG AGGACTAGTG AACATCAAGT AGACCTCAAG 
GTGGACCCTT CTCAGCCTTC GAATGTCAGT CACAAACTGT GGACAGCCGC TGGTTCCGGC 
CATCAGATAC TTCCTTTCCA TGAAAGCAGC AAGGATTCTT GTAAAAATGG CTCCAGTATG 
ACAAAGTGCA GTTGTACGTT GACATCTCCC CCGGGACTGT GGACTGGAAC CACATCTACT 
TTGAAAGATT TGGACACCTC CCACACTGAG TCCCCAACCC CCCATGACTC AAATTCACAC 
CGCCCTCAAC TGCAGGCGTT TATAACAGAA GCAAAGCTAA ATCTACCGAC AAGGACAATG 
ACCAGACAAG ATGGGGAAAC TGGAGATGGA GCCAAAGAAA ATATTGTTTA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005248625.1
CDS	1553..5809
Translation	MDSDSDSPFNYSWPSFPKMKIRRRTSKQDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	XM_005248568.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005248568.4

ORF Insert Sequence:

ATGGATTCTG ACTCCGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTTCC CAAAATGAAG 
ATTCGAAGAA GGACGTCCAA ACAAGATCGC TCCTTCGATA TCCTAAAAAA ATCCAAGCCG 
CCCTCGACAT TGCTTGCTGC AGGCCGGCTT TCAGACATGC TGAATGGAGG TGATGAAGTC 
TACGCTAACT GTATGGTGAT TGATCAGGTT GGTGATTTGG ATATCAGCTA TATTAATATA 
GAGGGAATCA CTGCCACTAC CAGCCCTGAA TCCAGAGGTT GCACTCTGTG GCCTCAGAGC 
AGCAAACACA CCCTTCCTAC AGAAACCAGT CCCAGTGTGT ACCCACTTAG TGAAAATGTC 
GAAGGGACAG CACACACTGA AGCCCAGCAG TCCTTCATGT CACCATCAAG TTCGTGTGCT 
TCCAACTTGA ATCTTTCTTT TGGTTGGCAT GGATTTGAAA AGGAACAAAG TCATCTAAAG 
AAAAGAAGTT CTAGCCTTGA TGCCTTGGAC GCCGACAGTG AAGGGGAAGG GCATTCTGAG 
CCATCCCACA TCTGTTACAC TCCAGGGTCT CAGAGCTCCT CAAGAACTGG GATTCCTAGT 
GGGGATGAAT TGGACTCTTT TGAGACTAAC ACTGAACCGG ATTTTAATAT CTCCAGGGCT 
GAATCCCTTC CTCTATCAAG TAATCTACAG TCGAAGGAAT CACTGCTTTC TGGAGTTCGC 
TCACGTTCTT ATTCTTGCTC ATCACCCAAA ATTTCTTTAG GAAAAACTCG TTTGGTGCGT 
GAATTAACAG TATGCAGTTC AAGTGAAGAG CAAAGAGCTT ACAGCTTATC GGAGCCACCA 
AGAGAAAACA GAATTCAGGA AGAAGAATGG GATAAATACA TCATACCTGC CAAATCAGAG 
TCTGAAAAAT ATAAAGTGAG TCGAACTTTC AGTTTCCTCA TGAATAGGAT GACTAGCCCT 
CGGAATAAAT CAAAGACAAA AAGCAAGGAT GCCAAAGATA AAGAGAAGCT GAATCGACAT 
CAGTTTGCCC CAGGAACATT CTCTGGGGTT CTGCAGTGTT TGGTTTGTGA TAAAACACTC 
CTGGGGAAAG AGTCACTGCA GTGTTCTAAC TGTAATGCAA ATGTGCACAA AGGTTGTAAA 
GATGCTGCGC CTGCATGCAC CAAGAAATTC CAAGAGAAAT ATAACAAGAA CAAACCACAG 
ACCATCCTTG GAAATTCTTC ATTTAGAGAC ATCCCACAGC CTGGTCTCTC CTTGCACCCT 
TCTTCCTCCG TGCCTGTTGG ATTGCCGACT GGAAGGAGGG AGACTGTGGG ACAGGTCCAT 
CCATTGTCCA GAAGTGTTCC AGGCACCACC TTGGAAAGCT TCAGGAGGTC AGCCACATCC 
TTGGAGTCTG AGAGTGACCA TAACAGCTGC AGAAGCAGGT CTCATTCTGA TGAGCTGCTA 
CAGTCCATGG GCTCTTCTCC CTCTACAGAG TCTTTCATAA TGGAAGATGT TGTGGATTCT 
TCTCTGTGGA GTGACCTCAG CAGTGATGCC CAGGAGTTTG AAGCAGAATC TTGGAGTCTT 
GTGGTGGATC CCTCATTTTG TAATAGGCAG GAGAAGGATG TCATCAAAAG ACAGGATGTC 
ATTTTTGAGC TAATGCAAAC AGAGATGCAT CACATCCAGA CCCTGTTCAT CATGTCTGAG 
ATCTTCAGGA AAGGCATGAA AGAGGAGCTG CAGCTGGACC ACAGCACCGT GGATAAAATT 
TTCCCCTGTT TAGATGAGTT GCTTGAAATC CACAGGCATT TCTTCTACAG TATGAAGGAA 
CGAAGGCAGG AATCCTGTGC TGGCAGCGAC AGGAATTTTG TGATCGACCG AATTGGAGAT 
ATTTTGGTAC AACAGTTTTC AGAAGAAAAT GCAAGTAAAA TGAAGAAAAT ATATGGAGAA 
TTCTGTTGCC ATCATAAAGA AGCTGTTAAC CTCTTTAAAG AACTCCAGCA GAATAAAAAG 
TTTCAGAATT TTATTAAGCT CCGAAATAGT AATCTTTTGG CTCGACGCCG AGGAATTCCA 
GAATGCATTC TGTTGGTCAC TCAGCGTATT ACAAAATACC CTGTCTTGGT GGAAAGGATA 
TTGCAGTACA CAAAGGAAAG AACTGAGGAA CATAAAGACT TACGCAAAGC GCTTTGCTTA 
ATTAAAGACA TGATTGCAAC AGTGGATTTA AAAGTCAATG AATATGAGAA AAACCAAAAA 
TGGCTTGAGA TCCTAAATAA GATTGAAAAC AAAACATACA CGAAGCTCAA AAATGGACAT 
GTGTTTAGGA AGCAGGCACT GATGAGTGAA GAAAGGACTC TGTTATATGA TGGCCTTGTT 
TACTGGAAAA CTGCTACAGG TCGTTTCAAA GATATCCTAG CTCTACTTCT AACTGATGTG 
CTGCTCTTTT TACAAGAAAA AGACCAGAAA TACATCTTTG CAGCCGTTGA TCAGAAGCCA 
TCAGTTATTT CCCTTCAAAA GCTTATTGCT AGAGAAGTTG CTAATGAGGA GAGAGGAATG 
TTTCTGATCA GTGCTTCATC TGCTGGTCCT GAGATGTATG AAATTCACAC CAATTCCAAG 
GAGGAACGCA ATAACTGGAT GAGACGGATC CAGCAGGCTG TAGAAAGTTG TCCTGAAGAA 
AAAGGGGGAA GGACAAGTGA ATCTGATGAA GACAAGAGGA AAGCTGAAGC CAGAGTGGCC 
AAAATTCAGC AATGTCAAGA AATACTCACT AACCAAGACC AACAAATTTG TGCGTATTTG 
GAGGAGAAGC TGCATATCTA TGCTGAACTT GGAGAACTGA GCGGATTTGA GGACGTCCAT 
CTAGAGCCCC ACCTCCTTAT TAAACCTGAC CCAGGCGAGC CTCCCCAGGC AGCCTCATTA 
CTGGCAGCAG CACTGAAAGA AGCTGAGAGC CTACAAGTTG CAGTGAAGGC CTCACAGATG 
GGCGCCGTGA GTCAATCATG TGAGGACAGT TGTGGAGACT CTGTCTTGGC GGACACACTC 
AGTTCTCATG ATGTACCAGG ATCACCGACT GCCTCATTAG TCACAGGAGG GAGAGAAGGA 
AGAGGCTGTT CGGATGTGGA TCCCGGGATC CAGGGTGTGG TAACCGACTT GGCCGTCTCT 
GATGCAGGGG AGAAGGTGGA ATGTAGAAAT TTTCCAGGTT CTTCACAATC AGAGATTATA 
CAAGCCATAC AGAATTTAAC CCGTCTCTTA TACAGCCTTC AGGCCGCCTT GACCATTCAG 
GACAGCCACA TTGAGATCCA CAGGCTGGTT CTCCAGCAGC AGGAGGGCCT GTCTCTCGGC 
CACTCTATCC TCCGAGGCGG CCCCTTGCAG GACCAGAAGT CTCGCGACGC GGACAGGCAG 
CATGAGGAGC TGGCCAATGT GCACCAGCTT CAGCACCAGC TCCAGCAGGA GCAGCGGCGC 
TGGCTGCGCA GGTGTGAGCA GCAGCAGCGG GCGCAGGCGA CCAGGGAGAG CTGGCTGCAG 
GAGCGGGAGC GGGAGTGCCA GTCGCAGGAG GAGCTGCTGC TGCGGAGCCG GGGCGAGCTG 
GACCTCCAGC TCCAGGAGTA CCAGCACAGC CTGGAGCGGC TGAGGGAGGG CCAGCGCCTG 
GTGGAGAGGG AGCAGGCGAG GATGCGGGCC CAGCAGAGCC TGCTGGGCCA CTGGAAGCAC 
GGCCGGCAGA GGAGCCTGCC CGCGGTGCTC CTTCCGGGTG GCCCCGAGGT AATGGAACTT 
AATCGATCTG AGAGTTTATG TCATGAAAAC TCATTCTTCA TCAATGAAGC TTTAGTACAA 
ATGTCATTTA ACACTTTCAA CAAACTGAAT CCATCAGTTA TCCATCAGGA TGCCACTTAC 
CCTACAACTC AATCTCATTC TGACTTGGTG AGGACTAGTG AACATCAAGT AGACCTCAAG 
GTGGACCCTT CTCAGCCTTC GAATGTCAGT CACAAACTGT GGACAGCCGC TGGTTCCGGC 
CATCAGATAC TTCCTTTCCA TGAAAGCAGC AAGGATTCTT GTAAAAATGG CTCCAGTATG 
ACAAAGTGCA GTTGTACGTT GACATCTCCC CCGGGACTGT GGACTGGAAC CACATCTACT 
TTGAAAGATT TGGACACCTC CCACACTGAG TCCCCAACCC CCCATGACTC AAATTCACAC 
CGCCCTCAAC TGCAGGCGTT TATAACAGAA GCAAAGCTAA ATCTACCGAC AAGGACAATG 
ACCAGACAAG ATGGGGAAAC TGGAGATGGA GCCAAAGAAA ATATTGTTTA CCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_011543547.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541849.1 Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

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CloneID	OHu71320
Clone ID Related Accession (Same CDS sequence)	XM_011543547.2
Accession Version	XM_011543547.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543547.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGAAGTG CAAAAGCCTG GAAAAGGGGG CAGATGATGA TCTATGCGAA GTTTGACAAA 
AATGTGTATC TTCCTGAAGA TGCTGAGTTT TACTTTACTT ATGACGGATC TCATCAGCGA 
CATGTCATGA TTGCAGAGCG CATCGAGGAT AACGTTCTCC AGTCCAGCGT CCCAGGCCAT 
GGGCTTCAGG AGACGGTGAC GGTATCTGTG TGCCTCTGCT CGGAAGGTTA CTCTCCGGTG 
ACCATGGGCT CTGGCTCAGT GACCTACGTG GACAACATGG CTTGCAGGCT GGCTCGTCTG 
CTGGTGACGC AGGCCAATCG CCTCACAGCC TGCAGCCACC AGACCCTGCT GACCCCATTT 
GCCTTGACGG CAGGAGCACT GCCTGCCTTG GATGAGGAGC TCGTGCTGGC TCTGACCCAT 
CTGGAATTGC CTCTAGAGTG GACTGTGTTG GGAAGTTCTT CACTTGAAGT ATCTTCTCAC 
AGAGAATCTC TTCTACACCT GGCTATGAGA TGGGGCCTGG CTAAACTTTC CCAGTTCTTC 
TTGTGTCTCC CGGGGGGAGT CCAGGCCTTG GCTTTACCCA ACGAAGAGGG TGCCACACCA 
TTAGACTTAG CTTTACGTGA AGGACACTCC AAGCTGGTGG AAGACGTCAC AAATTTTCAG 
GGCAGATGGT CCCCAAGCTT CTCCCGAGTG CAGCTCAGTG AAGAAGCCTC CTTGCATTAC 
ATTCACTCAT CGGAAACGCT GACCCTGACC CTGAACCACA CAGCCGAGCA TTTGTTGGAG 
GCAGATATTA AACTCTTCCG GAAATACTTT TGGGATAGAG CCTTTCTTGT CAAGGCCTTT 
GAGCCAGAAG CCAGGCCAGA GGAAAGAACA GCTATGCCCT CCAGCGGTGC AGAAACTGAA 
GAAGAGATTA AGAATTCAGT GTCCAGCAGA TCAGCAGCTG AAAAGGAAGA TATAAAGCGT 
GTCAAAAGCC TGGTGGTTCA ACACAATGAA CATGAAGACC AGCACAGCCT AGATTTGGAT 
CGCTCCTTCG ATATCCTAAA AAAATCCAAG CCGCCCTCGA CATTGCTTGC TGCAGGCCGG 
CTTTCAGACA TGCTGAATGG AGGTGATGAA GTCTACGCTA ACTGTATGGT GATTGATCAG 
GTTGGTGATT TGGATATCAG CTATATTAAT ATAGAGGGAA TCACTGCCAC TACCAGCCCT 
GAATCCAGAG GTTGCACTCT GTGGCCTCAG AGCAGCAAAC ACACCCTTCC TACAGAAACC 
AGTCCCAGTG TGTACCCACT TAGTGAAAAT GTCGAAGGGA CAGCACACAC TGAAGCCCAG 
CAGTCCTTCA TGTCACCATC AAGTTCGTGT GCTTCCAACT TGAATCTTTC TTTTGGTTGG 
CATGGATTTG AAAAGGAACA AAGTCATCTA AAGAAAAGAA GTTCTAGCCT TGATGCCTTG 
GACGCCGACA GTGAAGGGGA AGGGCATTCT GAGCCATCCC ACATCTGTTA CACTCCAGGG 
TCTCAGAGCT CCTCAAGAAC TGGGATTCCT AGTGGGGATG AATTGGACTC TTTTGAGACT 
AACACTGAAC CGGATTTTAA TATCTCCAGG GCTGAATCCC TTCCTCTATC AAGTAATCTA 
CAGTCGAAGG AATCACTGCT TTCTGGAGTT CGCTCACGTT CTTATTCTTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGTTTGGTG CGTGAATTAA CAGTATGCAG TTCAAGTGAA 
GAGCAAAGAG CTTACAGCTT ATCGGAGCCA CCAAGAGAAA ACAGAATTCA GGAAGAAGAA 
TGGGATAAAT ACATCATACC TGCCAAATCA GAGTCTGAAA AATATAAAGT GAGTCGAACT 
TTCAGTTTCC TCATGAATAG GATGACTAGC CCTCGGAATA AATCAAAGAC AAAAAGCAAG 
GATGCCAAAG ATAAAGAGAA GCTGAATCGA CATCAGTTTG CCCCAGGAAC ATTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG TGATAAAACA CTCCTGGGGA AAGAGTCACT GCAGTGTTCT 
AACTGTAATG CAAATGTGCA CAAAGGTTGT AAAGATGCTG CGCCTGCATG CACCAAGAAA 
TTCCAAGAGA AATATAACAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCATTTAGA 
GACATCCCAC AGCCTGGTCT CTCCTTGCAC CCTTCTTCCT CCGTGCCTGT TGGATTGCCG 
ACTGGAAGGA GGGAGACTGT GGGACAGGTC CATCCATTGT CCAGAAGTGT TCCAGGCACC 
ACCTTGGAAA GCTTCAGGAG GTCAGCCACA TCCTTGGAGT CTGAGAGTGA CCATAACAGC 
TGCAGAAGCA GGTCTCATTC TGATGAGCTG CTACAGTCCA TGGGCTCTTC TCCCTCTACA 
GAGTCTTTCA TAATGGAAGA TGTTGTGGAT TCTTCTCTGT GGAGTGACCT CAGCAGTGAT 
GCCCAGGAGT TTGAAGCAGA ATCTTGGAGT CTTGTGGTGG ATCCCTCATT TTGTAATAGG 
CAGGAGAAGG ATGTCATCAA AAGACAGGAT GTCATTTTTG AGCTAATGCA AACAGAGATG 
CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGCAT GAAAGAGGAG 
CTGCAGCTGG ACCACAGCAC CGTGGATAAA ATTTTCCCCT GTTTAGATGA GTTGCTTGAA 
ATCCACAGGC ATTTCTTCTA CAGTATGAAG GAACGAAGGC AGGAATCCTG TGCTGGCAGC 
GACAGGAATT TTGTGATCGA CCGAATTGGA GATATTTTGG TACAACAGTT TTCAGAAGAA 
AATGCAAGTA AAATGAAGAA AATATATGGA GAATTCTGTT GCCATCATAA AGAAGCTGTT 
AACCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT 
AGTAATCTTT TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGTTGGT CACTCAGCGT 
ATTACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA AAGAACTGAG 
GAACATAAAG ACTTACGCAA AGCGCTTTGC TTAATTAAAG ACATGATTGC AACAGTGGAT 
TTAAAAGTCA ATGAATATGA GAAAAACCAA AAATGGCTTG AGATCCTAAA TAAGATTGAA 
AACAAAACAT ACACGAAGCT CAAAAATGGA CATGTGTTTA GGAAGCAGGC ACTGATGAGT 
GAAGAAAGGA CTCTGTTATA TGATGGCCTT GTTTACTGGA AAACTGCTAC AGGTCGTTTC 
AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG 
AAATACATCT TTGCAGCCGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA AAAGCTTATT 
GCTAGAGAAG TTGCTAATGA GGAGAGAGGA ATGTTTCTGA TCAGTGCTTC ATCTGCTGGT 
CCTGAGATGT ATGAAATTCA CACCAATTCC AAGGAGGAAC GCAATAACTG GATGAGACGG 
ATCCAGCAGG CTGTAGAAAG TTGTCCTGAA GAAAAAGGGG GAAGGACAAG TGAATCTGAT 
GAAGACAAGA GGAAAGCTGA AGCCAGAGTG GCCAAAATTC AGCAATGTCA AGAAATACTC 
ACTAACCAAG ACCAACAAAT TTGTGCGTAT TTGGAGGAGA AGCTGCATAT CTATGCTGAA 
CTTGGAGAAC TGAGCGGATT TGAGGACGTC CATCTAGAGC CCCACCTCCT TATTAAACCT 
GACCCAGGCG AGCCTCCCCA GGCAGCCTCA TTACTGGCAG CAGCACTGAA AGAAGCTGAG 
AGCCTACAAG TTGCAGTGAA GGCCTCACAG ATGGGCGCCG TGAGTCAATC ATGTGAGGAC 
AGTTGTGGAG ACTCTGTCTT GGCGGACACA CTCAGTTCTC ATGATGTACC AGGATCACCG 
ACTGCCTCAT TAGTCACAGG AGGGAGAGAA GGAAGAGGCT GTTCGGATGT GGATCCCGGG 
ATCCAGGGTG TGGTAACCGA CTTGGCCGTC TCTGATGCAG GGGAGAAGGT GGAATGTAGA 
AATTTTCCAG GTTCTTCACA ATCAGAGATT ATACAAGCCA TACAGAATTT AACCCGTCTC 
TTATACAGCC TTCAGGCCGC CTTGACCATT CAGGACAGCC ACATTGAGAT CCACAGGCTG 
GTTCTCCAGC AGCAGGAGGG CCTGTCTCTC GGCCACTCTA TCCTCCGAGG CGGCCCCTTG 
CAGGACCAGA AGTCTCGCGA CGCGGACAGG CAGCATGAGG AGCTGGCCAA TGTGCACCAG 
CTTCAGCACC AGCTCCAGCA GGAGCAGCGG CGCTGGCTGC GCAGGTGTGA GCAGCAGCAG 
CGGGCGCAGG CGACCAGGGA GAGCTGGCTG CAGGAGCGGG AGCGGGAGTG CCAGTCGCAG 
GAGGAGCTGC TGCTGCGGAG CCGGGGCGAG CTGGACCTCC AGCTCCAGGA GTACCAGCAC 
AGCCTGGAGC GGCTGAGGGA GGGCCAGCGC CTGGTGGAGA GGGAGCAGGC GAGGATGCGG 
GCCCAGCAGA GCCTGCTGGG CCACTGGAAG CACGGCCGGC AGAGGAGCCT GCCCGCGGTG 
CTCCTTCCGG GTGGCCCCGA GGTAATGGAA CTTAATCGAT CTGAGAGTTT ATGTCATGAA 
AACTCATTCT TCATCAATGA AGCTTTAGTA CAAATGTCAT TTAACACTTT CAACAAACTG 
AATCCATCAG TTATCCATCA GGATGCCACT TACCCTACAA CTCAATCTCA TTCTGACTTG 
GTGAGGACTA GTGAACATCA AGTAGACCTC AAGGTGGACC CTTCTCAGCC TTCGAATGTC 
AGTCACAAAC TGTGGACAGC CGCTGGTTCC GGCCATCAGA TACTTCCTTT CCATGAAAGC 
AGCAAGGATT CTTGTAAAAA TGGCTCCAGT ATGACAAAGT GCAGTTGTAC GTTGACATCT 
CCCCCGGGAC TGTGGACTGG AACCACATCT ACTTTGAAAG ATTTGGACAC CTCCCACACT 
GAGTCCCCAA CCCCCCATGA CTCAAATTCA CACCGCCCTC AACTGCAGGC GTTTATAACA 
GAAGCAAAGC TAAATCTACC GACAAGGACA ATGACCAGAC AAGATGGGGA AACTGGAGAT 
GGAGCCAAAG AAAATATTGT TTACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541849.1
CDS	417..5606
Translation	MRSAKAWKRGQMMIYAKFDKNVYLPEDAEFYFTYDGSHQRHVMIAERIEDNVLQSSVPGHGLQETVTVSVCLCSEGYSPVTMGSGSVTYVDNMACRLARLLVTQANRLTACSHQTLLTPFALTAGALPALDEELVLALTHLELPLEWTVLGSSSLEVSSHRESLLHLAMRWGLAKLSQFFLCLPGGVQALALPNEEGATPLDLALREGHSKLVEDVTNFQGRWSPSFSRVQLSEEASLHYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLVKAFEPEARPEERTAMPSSGAETEEEIKNSVSSRSAAEKEDIKRVKSLVVQHNEHEDQHSLDLDRSFDILKKSKPPSTLLAAGRLSDMLNGGDEVYANCMVIDQVGDLDISYINIEGITATTSPESRGCTLWPQSSKHTLPTETSPSVYPLSENVEGTAHTEAQQSFMSPSSSCASNLNLSFGWHGFEKEQSHLKKRSSSLDALDADSEGEGHSEPSHICYTPGSQSSSRTGIPSGDELDSFETNTEPDFNISRAESLPLSSNLQSKESLLSGVRSRSYSCSSPKISLGKTRLVRELTVCSSSEEQRAYSLSEPPRENRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKSKDAKDKEKLNRHQFAPGTFSGVLQCLVCDKTLLGKESLQCSNCNANVHKGCKDAAPACTKKFQEKYNKNKPQTILGNSSFRDIPQPGLSLHPSSSVPVGLPTGRRETVGQVHPLSRSVPGTTLESFRRSATSLESESDHNSCRSRSHSDELLQSMGSSPSTESFIMEDVVDSSLWSDLSSDAQEFEAESWSLVVDPSFCNRQEKDVIKRQDVIFELMQTEMHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHRHFFYSMKERRQESCAGSDRNFVIDRIGDILVQQFSEENASKMKKIYGEFCCHHKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKERTEEHKDLRKALCLIKDMIATVDLKVNEYEKNQKWLEILNKIENKTYTKLKNGHVFRKQALMSEERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNNWMRRIQQAVESCPEEKGGRTSESDEDKRKAEARVAKIQQCQEILTNQDQQICAYLEEKLHIYAELGELSGFEDVHLEPHLLIKPDPGEPPQAASLLAAALKEAESLQVAVKASQMGAVSQSCEDSCGDSVLADTLSSHDVPGSPTASLVTGGREGRGCSDVDPGIQGVVTDLAVSDAGEKVECRNFPGSSQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQQEGLSLGHSILRGGPLQDQKSRDADRQHEELANVHQLQHQLQQEQRRWLRRCEQQQRAQATRESWLQERERECQSQEELLLRSRGELDLQLQEYQHSLERLREGQRLVEREQARMRAQQSLLGHWKHGRQRSLPAVLLPGGPEVMELNRSESLCHENSFFINEALVQMSFNTFNKLNPSVIHQDATYPTTQSHSDLVRTSEHQVDLKVDPSQPSNVSHKLWTAAGSGHQILPFHESSKDSCKNGSSMTKCSCTLTSPPGLWTGTTSTLKDLDTSHTESPTPHDSNSHRPQLQAFITEAKLNLPTRTMTRQDGETGDGAKENIVYL

Target ORF information:

RefSeq Version	XM_011543547.2
Organism	Homo sapiens(human)
Definition	Homo sapiens Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543547.2

ORF Insert Sequence:

ATGAGAAGTG CAAAAGCCTG GAAAAGGGGG CAGATGATGA TCTATGCGAA GTTTGACAAA 
AATGTGTATC TTCCTGAAGA TGCTGAGTTT TACTTTACTT ATGACGGATC TCATCAGCGA 
CATGTCATGA TTGCAGAGCG CATCGAGGAT AACGTTCTCC AGTCCAGCGT CCCAGGCCAT 
GGGCTTCAGG AGACGGTGAC GGTATCTGTG TGCCTCTGCT CGGAAGGTTA CTCTCCGGTG 
ACCATGGGCT CTGGCTCAGT GACCTACGTG GACAACATGG CTTGCAGGCT GGCTCGTCTG 
CTGGTGACGC AGGCCAATCG CCTCACAGCC TGCAGCCACC AGACCCTGCT GACCCCATTT 
GCCTTGACGG CAGGAGCACT GCCTGCCTTG GATGAGGAGC TCGTGCTGGC TCTGACCCAT 
CTGGAATTGC CTCTAGAGTG GACTGTGTTG GGAAGTTCTT CACTTGAAGT ATCTTCTCAC 
AGAGAATCTC TTCTACACCT GGCTATGAGA TGGGGCCTGG CTAAACTTTC CCAGTTCTTC 
TTGTGTCTCC CGGGGGGAGT CCAGGCCTTG GCTTTACCCA ACGAAGAGGG TGCCACACCA 
TTAGACTTAG CTTTACGTGA AGGACACTCC AAGCTGGTGG AAGACGTCAC AAATTTTCAG 
GGCAGATGGT CCCCAAGCTT CTCCCGAGTG CAGCTCAGTG AAGAAGCCTC CTTGCATTAC 
ATTCACTCAT CGGAAACGCT GACCCTGACC CTGAACCACA CAGCCGAGCA TTTGTTGGAG 
GCAGATATTA AACTCTTCCG GAAATACTTT TGGGATAGAG CCTTTCTTGT CAAGGCCTTT 
GAGCCAGAAG CCAGGCCAGA GGAAAGAACA GCTATGCCCT CCAGCGGTGC AGAAACTGAA 
GAAGAGATTA AGAATTCAGT GTCCAGCAGA TCAGCAGCTG AAAAGGAAGA TATAAAGCGT 
GTCAAAAGCC TGGTGGTTCA ACACAATGAA CATGAAGACC AGCACAGCCT AGATTTGGAT 
CGCTCCTTCG ATATCCTAAA AAAATCCAAG CCGCCCTCGA CATTGCTTGC TGCAGGCCGG 
CTTTCAGACA TGCTGAATGG AGGTGATGAA GTCTACGCTA ACTGTATGGT GATTGATCAG 
GTTGGTGATT TGGATATCAG CTATATTAAT ATAGAGGGAA TCACTGCCAC TACCAGCCCT 
GAATCCAGAG GTTGCACTCT GTGGCCTCAG AGCAGCAAAC ACACCCTTCC TACAGAAACC 
AGTCCCAGTG TGTACCCACT TAGTGAAAAT GTCGAAGGGA CAGCACACAC TGAAGCCCAG 
CAGTCCTTCA TGTCACCATC AAGTTCGTGT GCTTCCAACT TGAATCTTTC TTTTGGTTGG 
CATGGATTTG AAAAGGAACA AAGTCATCTA AAGAAAAGAA GTTCTAGCCT TGATGCCTTG 
GACGCCGACA GTGAAGGGGA AGGGCATTCT GAGCCATCCC ACATCTGTTA CACTCCAGGG 
TCTCAGAGCT CCTCAAGAAC TGGGATTCCT AGTGGGGATG AATTGGACTC TTTTGAGACT 
AACACTGAAC CGGATTTTAA TATCTCCAGG GCTGAATCCC TTCCTCTATC AAGTAATCTA 
CAGTCGAAGG AATCACTGCT TTCTGGAGTT CGCTCACGTT CTTATTCTTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGTTTGGTG CGTGAATTAA CAGTATGCAG TTCAAGTGAA 
GAGCAAAGAG CTTACAGCTT ATCGGAGCCA CCAAGAGAAA ACAGAATTCA GGAAGAAGAA 
TGGGATAAAT ACATCATACC TGCCAAATCA GAGTCTGAAA AATATAAAGT GAGTCGAACT 
TTCAGTTTCC TCATGAATAG GATGACTAGC CCTCGGAATA AATCAAAGAC AAAAAGCAAG 
GATGCCAAAG ATAAAGAGAA GCTGAATCGA CATCAGTTTG CCCCAGGAAC ATTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG TGATAAAACA CTCCTGGGGA AAGAGTCACT GCAGTGTTCT 
AACTGTAATG CAAATGTGCA CAAAGGTTGT AAAGATGCTG CGCCTGCATG CACCAAGAAA 
TTCCAAGAGA AATATAACAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCATTTAGA 
GACATCCCAC AGCCTGGTCT CTCCTTGCAC CCTTCTTCCT CCGTGCCTGT TGGATTGCCG 
ACTGGAAGGA GGGAGACTGT GGGACAGGTC CATCCATTGT CCAGAAGTGT TCCAGGCACC 
ACCTTGGAAA GCTTCAGGAG GTCAGCCACA TCCTTGGAGT CTGAGAGTGA CCATAACAGC 
TGCAGAAGCA GGTCTCATTC TGATGAGCTG CTACAGTCCA TGGGCTCTTC TCCCTCTACA 
GAGTCTTTCA TAATGGAAGA TGTTGTGGAT TCTTCTCTGT GGAGTGACCT CAGCAGTGAT 
GCCCAGGAGT TTGAAGCAGA ATCTTGGAGT CTTGTGGTGG ATCCCTCATT TTGTAATAGG 
CAGGAGAAGG ATGTCATCAA AAGACAGGAT GTCATTTTTG AGCTAATGCA AACAGAGATG 
CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGCAT GAAAGAGGAG 
CTGCAGCTGG ACCACAGCAC CGTGGATAAA ATTTTCCCCT GTTTAGATGA GTTGCTTGAA 
ATCCACAGGC ATTTCTTCTA CAGTATGAAG GAACGAAGGC AGGAATCCTG TGCTGGCAGC 
GACAGGAATT TTGTGATCGA CCGAATTGGA GATATTTTGG TACAACAGTT TTCAGAAGAA 
AATGCAAGTA AAATGAAGAA AATATATGGA GAATTCTGTT GCCATCATAA AGAAGCTGTT 
AACCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT 
AGTAATCTTT TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGTTGGT CACTCAGCGT 
ATTACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA AAGAACTGAG 
GAACATAAAG ACTTACGCAA AGCGCTTTGC TTAATTAAAG ACATGATTGC AACAGTGGAT 
TTAAAAGTCA ATGAATATGA GAAAAACCAA AAATGGCTTG AGATCCTAAA TAAGATTGAA 
AACAAAACAT ACACGAAGCT CAAAAATGGA CATGTGTTTA GGAAGCAGGC ACTGATGAGT 
GAAGAAAGGA CTCTGTTATA TGATGGCCTT GTTTACTGGA AAACTGCTAC AGGTCGTTTC 
AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG 
AAATACATCT TTGCAGCCGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA AAAGCTTATT 
GCTAGAGAAG TTGCTAATGA GGAGAGAGGA ATGTTTCTGA TCAGTGCTTC ATCTGCTGGT 
CCTGAGATGT ATGAAATTCA CACCAATTCC AAGGAGGAAC GCAATAACTG GATGAGACGG 
ATCCAGCAGG CTGTAGAAAG TTGTCCTGAA GAAAAAGGGG GAAGGACAAG TGAATCTGAT 
GAAGACAAGA GGAAAGCTGA AGCCAGAGTG GCCAAAATTC AGCAATGTCA AGAAATACTC 
ACTAACCAAG ACCAACAAAT TTGTGCGTAT TTGGAGGAGA AGCTGCATAT CTATGCTGAA 
CTTGGAGAAC TGAGCGGATT TGAGGACGTC CATCTAGAGC CCCACCTCCT TATTAAACCT 
GACCCAGGCG AGCCTCCCCA GGCAGCCTCA TTACTGGCAG CAGCACTGAA AGAAGCTGAG 
AGCCTACAAG TTGCAGTGAA GGCCTCACAG ATGGGCGCCG TGAGTCAATC ATGTGAGGAC 
AGTTGTGGAG ACTCTGTCTT GGCGGACACA CTCAGTTCTC ATGATGTACC AGGATCACCG 
ACTGCCTCAT TAGTCACAGG AGGGAGAGAA GGAAGAGGCT GTTCGGATGT GGATCCCGGG 
ATCCAGGGTG TGGTAACCGA CTTGGCCGTC TCTGATGCAG GGGAGAAGGT GGAATGTAGA 
AATTTTCCAG GTTCTTCACA ATCAGAGATT ATACAAGCCA TACAGAATTT AACCCGTCTC 
TTATACAGCC TTCAGGCCGC CTTGACCATT CAGGACAGCC ACATTGAGAT CCACAGGCTG 
GTTCTCCAGC AGCAGGAGGG CCTGTCTCTC GGCCACTCTA TCCTCCGAGG CGGCCCCTTG 
CAGGACCAGA AGTCTCGCGA CGCGGACAGG CAGCATGAGG AGCTGGCCAA TGTGCACCAG 
CTTCAGCACC AGCTCCAGCA GGAGCAGCGG CGCTGGCTGC GCAGGTGTGA GCAGCAGCAG 
CGGGCGCAGG CGACCAGGGA GAGCTGGCTG CAGGAGCGGG AGCGGGAGTG CCAGTCGCAG 
GAGGAGCTGC TGCTGCGGAG CCGGGGCGAG CTGGACCTCC AGCTCCAGGA GTACCAGCAC 
AGCCTGGAGC GGCTGAGGGA GGGCCAGCGC CTGGTGGAGA GGGAGCAGGC GAGGATGCGG 
GCCCAGCAGA GCCTGCTGGG CCACTGGAAG CACGGCCGGC AGAGGAGCCT GCCCGCGGTG 
CTCCTTCCGG GTGGCCCCGA GGTAATGGAA CTTAATCGAT CTGAGAGTTT ATGTCATGAA 
AACTCATTCT TCATCAATGA AGCTTTAGTA CAAATGTCAT TTAACACTTT CAACAAACTG 
AATCCATCAG TTATCCATCA GGATGCCACT TACCCTACAA CTCAATCTCA TTCTGACTTG 
GTGAGGACTA GTGAACATCA AGTAGACCTC AAGGTGGACC CTTCTCAGCC TTCGAATGTC 
AGTCACAAAC TGTGGACAGC CGCTGGTTCC GGCCATCAGA TACTTCCTTT CCATGAAAGC 
AGCAAGGATT CTTGTAAAAA TGGCTCCAGT ATGACAAAGT GCAGTTGTAC GTTGACATCT 
CCCCCGGGAC TGTGGACTGG AACCACATCT ACTTTGAAAG ATTTGGACAC CTCCCACACT 
GAGTCCCCAA CCCCCCATGA CTCAAATTCA CACCGCCCTC AACTGCAGGC GTTTATAACA 
GAAGCAAAGC TAAATCTACC GACAAGGACA ATGACCAGAC AAGATGGGGA AACTGGAGAT 
GGAGCCAAAG AAAATATTGT TTACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ARHGEF28
Entrez Gene ID	64283
Full Name	Rho guanine nucleotide exchange factor 28
Synonyms	RGNEF,RIP2,p190RHOGEF
General protein information	Preferred Names Rho guanine nucleotide exchange factor 28 Names rho guanine nucleotide exchange factor 28 190 kDa guanine nucleotide exchange factor Rho guanine nucleotide exchange factor (GEF) 28 Rho interacting protein 2 p190-RhoGEF rho-guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q13.2
Summary	This gene encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010].
Disorder MIM:	612790

Pan troglodytes (chimpanzee)	ARHGEF28	XP_001151239.2
Rattus norvegicus (Norway rat)	Arhgef28	NP_001102012.1
Gallus gallus (chicken)	LOC427201	XP_424789.4
Danio rerio (zebrafish)	arhgef28	XP_688428.4
Homo sapiens (human)	ARHGEF28	NP_001073948.2
Bos taurus (cattle)	RGNEF	NP_001095777.2
	ARHGEF28	XP_001101425.1
Canis lupus familiaris (dog)	ARHGEF28	XP_005617529.1
Mus musculus (house mouse)	Arhgef28	NP_036156.2
Xenopus tropicalis (tropical clawed frog)	arhgef28	XP_002934885.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ARHGEF28 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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