LOC427201 gene cDNA ORF clone, Gallus gallus(chicken)

mRNA	Protein	Name
XM_015277659.1	XP_015133145.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_025144888.1	XP_025000656.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_015277657.2	XP_015133143.1	rho guanine nucleotide exchange factor 28 isoform X3
XM_015277657.1	XP_015133143.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_015277655.1	XP_015133141.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_025144889.1	XP_025000657.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_015277658.2	XP_015133144.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_015277659.2	XP_015133145.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_015277658.1	XP_015133144.1	rho guanine nucleotide exchange factor 28 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa47914	XM_015277658.2 Latest version!	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa47915	XM_015277659.2 Latest version!	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa153053	XM_025144889.1 Latest version!	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OGa47913	XM_015277657.2 Latest version!	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa21189	XM_015277655.1 Latest version!	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OGa21189	XM_025144888.1 Latest version!	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
OGa47913	XM_015277657.1	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OGa47915	XM_015277659.1	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OGa47914	XM_015277658.1	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
Hide Old Accession Versions >>

LOC427201 ( XM_015277658.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133144.1 Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa47914
Clone ID Related Accession (Same CDS sequence)	XM_015277658.2 , XM_015277658.1
Accession Version	XM_015277658.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277658.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC 
CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA 
TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT 
ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG 
ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC 
AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT 
ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG 
ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG 
TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA 
CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG 
AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA 
AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC 
AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG 
GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG 
GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC 
GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC 
CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC 
AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC 
TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT 
GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA 
ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA 
GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG 
GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC 
CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC 
CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT 
GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT 
ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA 
CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA 
GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT 
GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA 
GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT 
GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA 
GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA 
CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG 
GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT 
ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA 
GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC 
GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG 
GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT 
GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA 
AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT 
ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG 
GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT 
GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC 
CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC 
CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA 
AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG 
AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG 
CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC 
CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG 
ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA 
GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA 
GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG 
GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC 
ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT 
GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT 
CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG 
GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133144.1
CDS	220..4461
Translation	MDSDSDSPFNYSWPSLPKMRIRRRASKQAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277658.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277658.2

ORF Insert Sequence:

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC 
CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA 
TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT 
ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG 
ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC 
AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT 
ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG 
ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG 
TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA 
CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG 
AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA 
AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC 
AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG 
GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG 
GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC 
GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC 
CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC 
AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC 
TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT 
GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA 
ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA 
GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG 
GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC 
CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC 
CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT 
GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT 
ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA 
CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA 
GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT 
GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA 
GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT 
GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA 
GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA 
CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG 
GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT 
ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA 
GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC 
GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG 
GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT 
GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA 
AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT 
ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG 
GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT 
GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC 
CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC 
CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA 
AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG 
AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG 
CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC 
CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG 
ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA 
GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA 
GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG 
GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC 
ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT 
GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT 
CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG 
GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_015277658.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133144.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa47914
Clone ID Related Accession (Same CDS sequence)	XM_015277658.2 , XM_015277658.1
Accession Version	XM_015277658.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC 
CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA 
TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT 
ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG 
ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC 
AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT 
ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG 
ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG 
TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA 
CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG 
AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA 
AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC 
AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG 
GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG 
GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC 
GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC 
CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC 
AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC 
TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT 
GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA 
ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA 
GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG 
GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC 
CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC 
CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT 
GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT 
ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA 
CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA 
GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT 
GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA 
GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT 
GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA 
GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA 
CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG 
GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT 
ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA 
GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC 
GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG 
GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT 
GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA 
AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT 
ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG 
GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT 
GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC 
CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC 
CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA 
AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG 
AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG 
CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC 
CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG 
ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA 
GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA 
GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG 
GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC 
ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT 
GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT 
CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG 
GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133144.1
CDS	155..4396
Translation	MDSDSDSPFNYSWPSLPKMRIRRRASKQAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277658.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277658.1

ORF Insert Sequence:

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GATGGTGTTC CCATGAACAG TGGCCGATCC 
CTCCTACAGG CTCTCTCACT CTCTAAGTCA GTGTCCCTGC TCCACCCCTG TAAACGAGCA 
TACAGCTTGC CTGAGCACTC CCGAGAGAAA AGGATTCAGG AGGAAGAATG GGATAAGTAT 
ATTGTACCTT CAAAAACAGA GTCTGAAAAA TACAAAGTGA GTCGGACTTT CAGCTTTCTG 
ATGAATAGAA TGACTAGCAC ACGGAATAAG TACAAGACAA AAAATAAAGA TACCAAAGAC 
AAAGAGAAGC TGAACAGACA TAATTTTATA AATGGAACCT TCTCTGGAAT TATGCCATGT 
ATGGCCTGTG AAAAGGCTCT CCTAGGAAAA GAGTCACTGC AGTGCTCTTA TTGCAATGTG 
ATTGTGCATA AGAACTGTAA GGAGTGTGTT CCTGTGTGCA CTAAGAAATC CCAGGAAAGG 
TACCATAGCA AAAACAAACC TCAAGTTGGC ATAGGAAATT TATCGTTTGG AGACATTTCA 
CAGCCTGGGA TTTCCTCAGT GCATCCTTCG TCCTCTGTAC CCATTGTATC TGCTGGGAGG 
AGGGAAGCCT TGCAGCAGTC CCATTCCTTG TCCAAAAGTG TCCCTGGTGC CAGTTTTGAA 
AGAAGATCTA TGCCGTTTCC TGAGCAGGAC TCTGATGCTA GCACATGGAG GACCAAGTCC 
AGATCTGAGG AGATGTTACA GGCGTTAGGG ACATATTCTT CAATGGACTC TTTTGTGATG 
GAAGTTGAGG TGGATTCGTC TCAGTGGACT GACATCAGTT CAGATGCACA CGAGTTTGAG 
GCGGAGTCAT GGAGTCTTGT TGTGGATCCG ATGTTCTGTA ACAAGCAAGA AAAGGATGTC 
GCCAAGAGGC AGGATGTCAT TTTTGAGCTG ATGCAAACAG AAGTTCATCA CATCCACACC 
CTGTTCATTA TGTCTGAAAT ATTCAGGAAG GGAATGAAAG AAGAACTGCA GCTGGACCAC 
AGCACGGTGG ACAGAATATT CCCCTGCTTA GATGAGCTAC TGGAGATCCA TAGGCAGTTC 
TTTTGTAGAA TGAGGGAGAG ACGGCAGGAA TCGTGTGAGG AGGGGAATGA GCGTAATTTT 
GTAATCAGCA GAATCGGAGA TATCCTTGTG CAACAGTTTT CAGAGGAAAA TGCAACTAAA 
ATGAAGAAAA TATATGGAGA ATTTTGCAGC CATCAGAAAG AAGCTGTTAA TCTCTTCAAA 
GAGTTGCAGC AAAACAAGAA GTTCCAGAAT TTTATTAAAC TGAGGAACAG CAATTTGTTG 
GCACGCCGCA GAGGAATCCC CGAATGCATT CTGCTTGTCA CTCAGCGAAT CACCAAATAC 
CCTGTTCTAG TTGAAAGGAT ACTGCAGTAC ACAAAAGAAG GAACAGAAGA ACATAAAGAC 
CTATGCAAAG CCCTTCGTTT AATTAAAGAC ATGATTGCAG CAGTAGATTT GAAAGTTAAT 
GAATACGAGA AAAGGCAAAA ACTGATGGAA ATTCTCAACC GAACTGAAAA CAAAACATAT 
ACAAAACTGA AAAGTGGACA CATTTTTAGG AAGCAAGACT TGATGAGGAA AGAGAGGATA 
CTTCTTCATG AAGGTTTAGT GTACTGGAAA ACAGCTACTG GTCGTTTCAA AGACACCCTA 
GCTCTGCTTC TAACTGATAT GCTGCTGTTC TTGCAAGAAA AAGATCAGAA GTACGTTTTT 
GCAGCTGTTG ACCAGAAGCC TTCAGTAATC TCTCTTCAGA AGCTCATTGT AAGAGAAGTA 
GCCAATGAAG AAAGGGGGAT GTTTCTGATT AGTGCTTCAT CTGCAGGACC TGAGATGTAT 
GAAGTTCATA CGAACTCCAG AGAAGAGCGT AACAGCTGGA TGAGGCATAT TCAAGATGCA 
GTGGAAAGTT GTCCAGAGGA ACAGGAAGAA GGAAAAATAG GTGAATTTGA TGAGGACAGA 
CGTATCGCTG AGGCTAAAGC ATGCAAAATT CAGAAATGTC AAGAATCACT GAGTAACCAG 
GACCAGCAAA TCTGCAGTTG CTTGGAAGAC AAATTGCATA TCTATGCAGA ACTAGTAGAT 
ATGAGTGGAT TTGAAGATGT TCATGTAGAA CCTCACCTCC TTATCAAACC TGATTATGGA 
GAAACTCCAC AGGCTTCTTC GTTGCTGGTA GCAGCCCTCA GAGAAGTTGA AAGTCTCCAC 
GTTGCTCTCA CGTCATCACA AGTGAATGAA ACGGACAGAT CATCAGAGGA AAGTACAGAG 
GAATTAACTT TGAAACACAG ACTTAGTGCC AGTGAAATCT TGGAATCTTT CCCTGGTGAT 
GCAGAAACAA AGGAAGTTGA AGAATCACCT GAAGTTGATC TGAGTGCTTG CTCTATTGAA 
AAGGAAATTT CTGATGCCAG TGTAGAGTAT AAGGGAGAAA GTACGAGTTT CCCAGGATCT 
ACAGGGACAG AGATTGTACA AGCAATCCAG AATCTAACTC GTCTCTTGTA CAGCATACAG 
GCAGCGTTAG CTATCCAGGA CAGCCACAGA GAGCTCCATA GGTTACTCCT GCAAGAGAAT 
GAGAAGACCA GTCGTGGCCA TAGTTCTCGG ATACCCCTCT TCCTGGAACC AGAAAAATAC 
CGTAATTCAG AAAAACAAAG GGAGGAGCTG GCAAACATCA ACAAACTCCA GCAGCAGTTC 
CAGCAAGAGC AGCAGCGCTG GCTGCGTGAA TGCGAGCAGC GGCAGCAAGA GCTGGAGGTA 
AGGGCAGGCC TGCTGGAGCA GAGGGAGCAG GCCTGCAGCT GCCAGGAGGA GCTGCTGGAG 
AAGAGCCGCC AGGGGCTTGC ACTGCAGTCG CAGGAGTTCC AGCAGAGCCT GGAGAGGCTG 
CGGGAAGGGC AGAAGTTGTT GGAGAAAAAA AGGGACAGTA TCAAAATGCA GAAGAAGCTC 
CTCTGGCACT GGAAGCATGG TCTCCAAAGC AGCCTGCTGC TGCCCACAGG GAGCCACGAG 
ATAATGGGAC ACGATCAGTT GGATAGCTTA CATGGAGAAA ATGCGTTCTA TTTAAATGAA 
GCTGTAGTAC GCGTGTCTCT AAGCGGCCTC AACAGATCAG ATTCTTCTCT TGTTTATGAA 
GATGGTGTGT ATGGGCTGGG CATTTCAGAC TTGGATATGG CAAGGACTTG TGAAAATCAG 
GTGAACCTCA GGGTGGATAC TCCTTGCCAA CCAGCATTGA CCAATGAACT GTGGAAATCC 
ACTGGCCCTT ACCAGCAGAT GTCTTTCTCC TGCAAAAGCA ACAAGGATGC TTTTAATAAT 
GATCTAAATG CATCACAGAC CCAGGGTCCC CAGGCAGGCA GTCCAAACCA TGGATCCATT 
CAGCCACCAC AAGTGGAACC GCTGATGCTG AACCACCAGC CTGAGAGCCT GCAGGATGCG 
GAAAGTGGAG ACGGAGGGGA GGAGAATATT GTTTACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_015277659.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133145.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X4, mRNA.

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CloneID	OGa47915
Clone ID Related Accession (Same CDS sequence)	XM_015277659.1 , XM_015277659.2
Accession Version	XM_015277659.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC 
CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG 
TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA 
CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT 
AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC 
CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG 
GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT 
CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG 
CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC 
CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT 
GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG 
GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT 
CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT 
GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT 
TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA 
TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC 
CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA 
CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT 
ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA 
TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG 
TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC 
GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA 
CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA 
ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA 
CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC 
ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG 
TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG 
CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT 
TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG 
TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA 
GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA 
CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA 
TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC 
TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT 
CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG 
TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA 
GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA 
CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA 
GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT 
GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA 
GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC 
AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT 
AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG 
GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG 
CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG 
AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA 
CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG 
GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT 
CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG 
GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA 
AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC 
ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT 
CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG 
TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC 
CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG 
CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG 
GAGAATATTG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133145.1
CDS	152..4312
Translation	MDSDSDSPFNYSWPSLPKMRIRRRASKQAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEERAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277659.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277659.1

ORF Insert Sequence:

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC 
CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG 
TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA 
CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT 
AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC 
CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG 
GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT 
CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG 
CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC 
CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT 
GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG 
GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT 
CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT 
GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT 
TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA 
TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC 
CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA 
CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT 
ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA 
TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG 
TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC 
GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA 
CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA 
ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA 
CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC 
ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG 
TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG 
CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT 
TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG 
TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA 
GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA 
CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA 
TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC 
TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT 
CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG 
TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA 
GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA 
CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA 
GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT 
GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA 
GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC 
AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT 
AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG 
GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG 
CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG 
AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA 
CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG 
GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT 
CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG 
GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA 
AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC 
ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT 
CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG 
TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC 
CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG 
CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG 
GAGAATATTG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_015277659.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133145.1 Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

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CloneID	OGa47915
Clone ID Related Accession (Same CDS sequence)	XM_015277659.1 , XM_015277659.2
Accession Version	XM_015277659.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277659.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC 
CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG 
TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA 
CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT 
AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC 
CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG 
GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT 
CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG 
CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC 
CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT 
GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG 
GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT 
CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT 
GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT 
TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA 
TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC 
CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA 
CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT 
ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA 
TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG 
TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC 
GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA 
CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA 
ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA 
CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC 
ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG 
TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG 
CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT 
TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG 
TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA 
GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA 
CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA 
TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC 
TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT 
CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG 
TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA 
GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA 
CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA 
GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT 
GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA 
GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC 
AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT 
AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG 
GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG 
CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG 
AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA 
CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG 
GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT 
CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG 
GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA 
AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC 
ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT 
CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG 
TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC 
CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG 
CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG 
GAGAATATTG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133145.1
CDS	219..4379
Translation	MDSDSDSPFNYSWPSLPKMRIRRRASKQAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEERAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277659.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277659.2

ORF Insert Sequence:

ATGGACTCAG ACTCTGATTC ACCATTTAAT TACTCTTGGC CTTCCCTCCC TAAAATGCGG 
ATTCGAAGAA GGGCATCTAA ACAAGCACAG AGACATTCTA CCCTTGATAT TCTTAAGAAG 
CTGAAAACTC CACCTACCTT CTTTGCTGCA GCCAGACTTT CTACTATGCT GAATGGAAGT 
GATGAAGTCT ATGCGAACTG CATGCTAGTA GACCAGGCTG AAGATTTAAA AATGAACTAC 
ATTCATGGAG ATAGCATTAG CAGTGAAACG TGTCTGAATC CCAGCAGCTC AGCCCCAGTA 
GCGAAGAGCT CTTCGTCTCC TTCCCTTGAG GAGAATGACC AGTATAGATG CGATCCAAGT 
GAAGGAAATC AGTCAGTGAA AAATGAAGGA AATACAGGCT TAAGACCTAC TGTTTCCCCA 
TCTGATCTGT ATGAAGCAGA TTCCTACCTT CCATTCAAGA CAAATGAGTT TATTAAGGAA 
CAGGGCCAAC TTCATGCTGT TATGAAAAAA AAAAGTTCAA GTCTCGATGA CCTTGAAGTA 
AACAGCAGAA GTGAAGGTGT CTTGGGCAGA TCCAATATAC CCTACCCTCT TCTTGAACAT 
CCAGAGGTGA AGGCTTGTAG CAGAGATGGA TTAGATTCTT CTGAGATGAA CATCAGCCGG 
ACAGAATCAC TGCCCTTATC TATCAGTCCA CAGACCAAGG AATGTTCACC CTCTGGAATT 
CGGTCACGCT CGTATTCCTG TTCCTCACCC AAAGACTCAT TGGGAAGACC TCGCATTCTT 
CAGGACTTCA CAGTTGGTGA TTCAGGTGAA GAACGAGCAT ACAGCTTGCC TGAGCACTCC 
CGAGAGAAAA GGATTCAGGA GGAAGAATGG GATAAGTATA TTGTACCTTC AAAAACAGAG 
TCTGAAAAAT ACAAAGTGAG TCGGACTTTC AGCTTTCTGA TGAATAGAAT GACTAGCACA 
CGGAATAAGT ACAAGACAAA AAATAAAGAT ACCAAAGACA AAGAGAAGCT GAACAGACAT 
AATTTTATAA ATGGAACCTT CTCTGGAATT ATGCCATGTA TGGCCTGTGA AAAGGCTCTC 
CTAGGAAAAG AGTCACTGCA GTGCTCTTAT TGCAATGTGA TTGTGCATAA GAACTGTAAG 
GAGTGTGTTC CTGTGTGCAC TAAGAAATCC CAGGAAAGGT ACCATAGCAA AAACAAACCT 
CAAGTTGGCA TAGGAAATTT ATCGTTTGGA GACATTTCAC AGCCTGGGAT TTCCTCAGTG 
CATCCTTCGT CCTCTGTACC CATTGTATCT GCTGGGAGGA GGGAAGCCTT GCAGCAGTCC 
CATTCCTTGT CCAAAAGTGT CCCTGGTGCC AGTTTTGAAA GAAGATCTAT GCCGTTTCCT 
GAGCAGGACT CTGATGCTAG CACATGGAGG ACCAAGTCCA GATCTGAGGA GATGTTACAG 
GCGTTAGGGA CATATTCTTC AATGGACTCT TTTGTGATGG AAGTTGAGGT GGATTCGTCT 
CAGTGGACTG ACATCAGTTC AGATGCACAC GAGTTTGAGG CGGAGTCATG GAGTCTTGTT 
GTGGATCCGA TGTTCTGTAA CAAGCAAGAA AAGGATGTCG CCAAGAGGCA GGATGTCATT 
TTTGAGCTGA TGCAAACAGA AGTTCATCAC ATCCACACCC TGTTCATTAT GTCTGAAATA 
TTCAGGAAGG GAATGAAAGA AGAACTGCAG CTGGACCACA GCACGGTGGA CAGAATATTC 
CCCTGCTTAG ATGAGCTACT GGAGATCCAT AGGCAGTTCT TTTGTAGAAT GAGGGAGAGA 
CGGCAGGAAT CGTGTGAGGA GGGGAATGAG CGTAATTTTG TAATCAGCAG AATCGGAGAT 
ATCCTTGTGC AACAGTTTTC AGAGGAAAAT GCAACTAAAA TGAAGAAAAT ATATGGAGAA 
TTTTGCAGCC ATCAGAAAGA AGCTGTTAAT CTCTTCAAAG AGTTGCAGCA AAACAAGAAG 
TTCCAGAATT TTATTAAACT GAGGAACAGC AATTTGTTGG CACGCCGCAG AGGAATCCCC 
GAATGCATTC TGCTTGTCAC TCAGCGAATC ACCAAATACC CTGTTCTAGT TGAAAGGATA 
CTGCAGTACA CAAAAGAAGG AACAGAAGAA CATAAAGACC TATGCAAAGC CCTTCGTTTA 
ATTAAAGACA TGATTGCAGC AGTAGATTTG AAAGTTAATG AATACGAGAA AAGGCAAAAA 
CTGATGGAAA TTCTCAACCG AACTGAAAAC AAAACATATA CAAAACTGAA AAGTGGACAC 
ATTTTTAGGA AGCAAGACTT GATGAGGAAA GAGAGGATAC TTCTTCATGA AGGTTTAGTG 
TACTGGAAAA CAGCTACTGG TCGTTTCAAA GACACCCTAG CTCTGCTTCT AACTGATATG 
CTGCTGTTCT TGCAAGAAAA AGATCAGAAG TACGTTTTTG CAGCTGTTGA CCAGAAGCCT 
TCAGTAATCT CTCTTCAGAA GCTCATTGTA AGAGAAGTAG CCAATGAAGA AAGGGGGATG 
TTTCTGATTA GTGCTTCATC TGCAGGACCT GAGATGTATG AAGTTCATAC GAACTCCAGA 
GAAGAGCGTA ACAGCTGGAT GAGGCATATT CAAGATGCAG TGGAAAGTTG TCCAGAGGAA 
CAGGAAGAAG GAAAAATAGG TGAATTTGAT GAGGACAGAC GTATCGCTGA GGCTAAAGCA 
TGCAAAATTC AGAAATGTCA AGAATCACTG AGTAACCAGG ACCAGCAAAT CTGCAGTTGC 
TTGGAAGACA AATTGCATAT CTATGCAGAA CTAGTAGATA TGAGTGGATT TGAAGATGTT 
CATGTAGAAC CTCACCTCCT TATCAAACCT GATTATGGAG AAACTCCACA GGCTTCTTCG 
TTGCTGGTAG CAGCCCTCAG AGAAGTTGAA AGTCTCCACG TTGCTCTCAC GTCATCACAA 
GTGAATGAAA CGGACAGATC ATCAGAGGAA AGTACAGAGG AATTAACTTT GAAACACAGA 
CTTAGTGCCA GTGAAATCTT GGAATCTTTC CCTGGTGATG CAGAAACAAA GGAAGTTGAA 
GAATCACCTG AAGTTGATCT GAGTGCTTGC TCTATTGAAA AGGAAATTTC TGATGCCAGT 
GTAGAGTATA AGGGAGAAAG TACGAGTTTC CCAGGATCTA CAGGGACAGA GATTGTACAA 
GCAATCCAGA ATCTAACTCG TCTCTTGTAC AGCATACAGG CAGCGTTAGC TATCCAGGAC 
AGCCACAGAG AGCTCCATAG GTTACTCCTG CAAGAGAATG AGAAGACCAG TCGTGGCCAT 
AGTTCTCGGA TACCCCTCTT CCTGGAACCA GAAAAATACC GTAATTCAGA AAAACAAAGG 
GAGGAGCTGG CAAACATCAA CAAACTCCAG CAGCAGTTCC AGCAAGAGCA GCAGCGCTGG 
CTGCGTGAAT GCGAGCAGCG GCAGCAAGAG CTGGAGGTAA GGGCAGGCCT GCTGGAGCAG 
AGGGAGCAGG CCTGCAGCTG CCAGGAGGAG CTGCTGGAGA AGAGCCGCCA GGGGCTTGCA 
CTGCAGTCGC AGGAGTTCCA GCAGAGCCTG GAGAGGCTGC GGGAAGGGCA GAAGTTGTTG 
GAGAAAAAAA GGGACAGTAT CAAAATGCAG AAGAAGCTCC TCTGGCACTG GAAGCATGGT 
CTCCAAAGCA GCCTGCTGCT GCCCACAGGG AGCCACGAGA TAATGGGACA CGATCAGTTG 
GATAGCTTAC ATGGAGAAAA TGCGTTCTAT TTAAATGAAG CTGTAGTACG CGTGTCTCTA 
AGCGGCCTCA ACAGATCAGA TTCTTCTCTT GTTTATGAAG ATGGTGTGTA TGGGCTGGGC 
ATTTCAGACT TGGATATGGC AAGGACTTGT GAAAATCAGG TGAACCTCAG GGTGGATACT 
CCTTGCCAAC CAGCATTGAC CAATGAACTG TGGAAATCCA CTGGCCCTTA CCAGCAGATG 
TCTTTCTCCT GCAAAAGCAA CAAGGATGCT TTTAATAATG ATCTAAATGC ATCACAGACC 
CAGGGTCCCC AGGCAGGCAG TCCAAACCAT GGATCCATTC AGCCACCACA AGTGGAACCG 
CTGATGCTGA ACCACCAGCC TGAGAGCCTG CAGGATGCGG AAAGTGGAGA CGGAGGGGAG 
GAGAATATTG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_025144889.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025000657.1 Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa153053
Clone ID Related Accession (Same CDS sequence)	XM_025144889.1
Accession Version	XM_025144889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTG CTCACGAAGA ATTGCCATCA ACGTGGAGCA TCCTCGGAAG CTGCTATGAA 
GGAGAGCCTA CACACAAAGA AACTCTTCTC CACCTTGCAA TGAAATTGGG CTTGGTTAAA 
CTTTCAGAGT TCTTAGTGGG CCAGCCTGGA GGGACATTGG CATTAGCGCT ACCTAATGAG 
GATGGAGCAA CACCATTAGA CTTGGCTGAG CAAAACGGGC ACTCCAAACT AGTTGAAGTC 
TTCTCAAATT TCCAGGGCAG CCGCTCGCCT GACGTTGCCA GAGTGGAGAT CAGCAAGGGG 
GCCTGCATGA GCTTTGTGCG CTCGTCAGCA GCTCTGACGC TGACGCTGGA CCACACGGCC 
AAGCACTTAC TGGAGTCAGA TGTGAGGCTC TTCAGGAAGT ACTTCTGGAA CAGGCCGCTG 
CTCCATGAGT GTATCAATTC AAACCAGCTT GAGCCAGTGG AAGATCCAGT AATGCGCTGC 
AGCACAACAA ACTCTGTAGA AGGTCCTGTG AGCTTGGCAC CTGATGACTT GTCCCAGGAT 
GGTGAGAAGC CTGTCCATGG TGCTGTGGTT CCTTCTAAAG AAGTTGAAGA TAATGTCCTG 
CTAGACATAG CACAGAGACA TTCTACCCTT GATATTCTTA AGAAGCTGAA AACTCCACCT 
ACCTTCTTTG CTGCAGCCAG ACTTTCTACT ATGCTGAATG GAAGTGATGA AGTCTATGCG 
AACTGCATGC TAGTAGACCA GGCTGAAGAT TTAAAAATGA ACTACATTCA TGGAGATAGC 
ATTAGCAGTG AAACGTGTCT GAATCCCAGC AGCTCAGCCC CAGTAGCGAA GAGCTCTTCG 
TCTCCTTCCC TTGAGGAGAA TGACCAGTAT AGATGCGATC CAAGTGAAGG AAATCAGTCA 
GTGAAAAATG AAGGAAATAC AGGCTTAAGA CCTACTGTTT CCCCATCTGA TCTGTATGAA 
GCAGATTCCT ACCTTCCATT CAAGACAAAT GAGTTTATTA AGGAACAGGG CCAACTTCAT 
GCTGTTATGA AAAAAAAAAG TTCAAGTCTC GATGACCTTG AAGTAAACAG CAGAAGTGAA 
GGTGTCTTGG GCAGATCCAA TATACCCTAC CCTCTTCTTG AACATCCAGA GGTGAAGGCT 
TGTAGCAGAG ATGGATTAGA TTCTTCTGAG ATGAACATCA GCCGGACAGA ATCACTGCCC 
TTATCTATCA GTCCACAGAC CAAGGAATGT TCACCCTCTG GAATTCGGTC ACGCTCGTAT 
TCCTGTTCCT CACCCAAAGA CTCATTGGGA AGACCTCGCA TTCTTCAGGA CTTCACAGTT 
GGTGATTCAG GTGAAGATGG TGTTCCCATG AACAGTGGCC GATCCCTCCT ACAGGCTCTC 
TCACTCTCTA AGTCAGTGTC CCTGCTCCAC CCCTGTAAAC GAGCATACAG CTTGCCTGAG 
CACTCCCGAG AGAAAAGGAT TCAGGAGGAA GAATGGGATA AGTATATTGT ACCTTCAAAA 
ACAGAGTCTG AAAAATACAA AGTGAGTCGG ACTTTCAGCT TTCTGATGAA TAGAATGACT 
AGCACACGGA ATAAGTACAA GACAAAAAAT AAAGATACCA AAGACAAAGA GAAGCTGAAC 
AGACATAATT TTATAAATGG AACCTTCTCT GGAATTATGC CATGTATGGC CTGTGAAAAG 
GCTCTCCTAG GAAAAGAGTC ACTGCAGTGC TCTTATTGCA ATGTGATTGT GCATAAGAAC 
TGTAAGGAGT GTGTTCCTGT GTGCACTAAG AAATCCCAGG AAAGGTACCA TAGCAAAAAC 
AAACCTCAAG TTGGCATAGG AAATTTATCG TTTGGAGACA TTTCACAGCC TGGGATTTCC 
TCAGTGCATC CTTCGTCCTC TGTACCCATT GTATCTGCTG GGAGGAGGGA AGCCTTGCAG 
CAGTCCCATT CCTTGTCCAA AAGTGTCCCT GGTGCCAGTT TTGAAAGAAG ATCTATGCCG 
TTTCCTGAGC AGGACTCTGA TGCTAGCACA TGGAGGACCA AGTCCAGATC TGAGGAGATG 
TTACAGGCGT TAGGGACATA TTCTTCAATG GACTCTTTTG TGATGGAAGT TGAGGTGGAT 
TCGTCTCAGT GGACTGACAT CAGTTCAGAT GCACACGAGT TTGAGGCGGA GTCATGGAGT 
CTTGTTGTGG ATCCGATGTT CTGTAACAAG CAAGAAAAGG ATGTCGCCAA GAGGCAGGAT 
GTCATTTTTG AGCTGATGCA AACAGAAGTT CATCACATCC ACACCCTGTT CATTATGTCT 
GAAATATTCA GGAAGGGAAT GAAAGAAGAA CTGCAGCTGG ACCACAGCAC GGTGGACAGA 
ATATTCCCCT GCTTAGATGA GCTACTGGAG ATCCATAGGC AGTTCTTTTG TAGAATGAGG 
GAGAGACGGC AGGAATCGTG TGAGGAGGGG AATGAGCGTA ATTTTGTAAT CAGCAGAATC 
GGAGATATCC TTGTGCAACA GTTTTCAGAG GAAAATGCAA CTAAAATGAA GAAAATATAT 
GGAGAATTTT GCAGCCATCA GAAAGAAGCT GTTAATCTCT TCAAAGAGTT GCAGCAAAAC 
AAGAAGTTCC AGAATTTTAT TAAACTGAGG AACAGCAATT TGTTGGCACG CCGCAGAGGA 
ATCCCCGAAT GCATTCTGCT TGTCACTCAG CGAATCACCA AATACCCTGT TCTAGTTGAA 
AGGATACTGC AGTACACAAA AGAAGGAACA GAAGAACATA AAGACCTATG CAAAGCCCTT 
CGTTTAATTA AAGACATGAT TGCAGCAGTA GATTTGAAAG TTAATGAATA CGAGAAAAGG 
CAAAAACTGA TGGAAATTCT CAACCGAACT GAAAACAAAA CATATACAAA ACTGAAAAGT 
GGACACATTT TTAGGAAGCA AGACTTGATG AGGAAAGAGA GGATACTTCT TCATGAAGGT 
TTAGTGTACT GGAAAACAGC TACTGGTCGT TTCAAAGACA CCCTAGCTCT GCTTCTAACT 
GATATGCTGC TGTTCTTGCA AGAAAAAGAT CAGAAGTACG TTTTTGCAGC TGTTGACCAG 
AAGCCTTCAG TAATCTCTCT TCAGAAGCTC ATTGTAAGAG AAGTAGCCAA TGAAGAAAGG 
GGGATGTTTC TGATTAGTGC TTCATCTGCA GGACCTGAGA TGTATGAAGT TCATACGAAC 
TCCAGAGAAG AGCGTAACAG CTGGATGAGG CATATTCAAG ATGCAGTGGA AAGTTGTCCA 
GAGGAACAGG AAGAAGGAAA AATAGGTGAA TTTGATGAGG ACAGACGTAT CGCTGAGGCT 
AAAGCATGCA AAATTCAGAA ATGTCAAGAA TCACTGAGTA ACCAGGACCA GCAAATCTGC 
AGTTGCTTGG AAGACAAATT GCATATCTAT GCAGAACTAG TAGATATGAG TGGATTTGAA 
GATGTTCATG TAGAACCTCA CCTCCTTATC AAACCTGATT ATGGAGAAAC TCCACAGGCT 
TCTTCGTTGC TGGTAGCAGC CCTCAGAGAA GTTGAAAGTC TCCACGTTGC TCTCACGTCA 
TCACAAGTGA ATGAAACGGA CAGATCATCA GAGGAAAGTA CAGAGGAATT AACTTTGAAA 
CACAGACTTA GTGCCAGTGA AATCTTGGAA TCTTTCCCTG GTGATGCAGA AACAAAGGAA 
GTTGAAGAAT CACCTGAAGT TGATCTGAGT GCTTGCTCTA TTGAAAAGGA AATTTCTGAT 
GCCAGTGTAG AGTATAAGGG AGAAAGTACG AGTTTCCCAG GATCTACAGG GACAGAGATT 
GTACAAGCAA TCCAGAATCT AACTCGTCTC TTGTACAGCA TACAGGCAGC GTTAGCTATC 
CAGGACAGCC ACAGAGAGCT CCATAGGTTA CTCCTGCAAG AGAATGAGAA GACCAGTCGT 
GGCCATAGTT CTCGGATACC CCTCTTCCTG GAACCAGAAA AATACCGTAA TTCAGAAAAA 
CAAAGGGAGG AGCTGGCAAA CATCAACAAA CTCCAGCAGC AGTTCCAGCA AGAGCAGCAG 
CGCTGGCTGC GTGAATGCGA GCAGCGGCAG CAAGAGCTGG AGGTAAGGGC AGGCCTGCTG 
GAGCAGAGGG AGCAGGCCTG CAGCTGCCAG GAGGAGCTGC TGGAGAAGAG CCGCCAGGGG 
CTTGCACTGC AGTCGCAGGA GTTCCAGCAG AGCCTGGAGA GGCTGCGGGA AGGGCAGAAG 
TTGTTGGAGA AAAAAAGGGA CAGTATCAAA ATGCAGAAGA AGCTCCTCTG GCACTGGAAG 
CATGGTCTCC AAAGCAGCCT GCTGCTGCCC ACAGGGAGCC ACGAGATAAT GGGACACGAT 
CAGTTGGATA GCTTACATGG AGAAAATGCG TTCTATTTAA ATGAAGCTGT AGTACGCGTG 
TCTCTAAGCG GCCTCAACAG ATCAGATTCT TCTCTTGTTT ATGAAGATGG TGTGTATGGG 
CTGGGCATTT CAGACTTGGA TATGGCAAGG ACTTGTGAAA ATCAGGTGAA CCTCAGGGTG 
GATACTCCTT GCCAACCAGC ATTGACCAAT GAACTGTGGA AATCCACTGG CCCTTACCAG 
CAGATGTCTT TCTCCTGCAA AAGCAACAAG GATGCTTTTA ATAATGATCT AAATGCATCA 
CAGACCCAGG GTCCCCAGGC AGGCAGTCCA AACCATGGAT CCATTCAGCC ACCACAAGTG 
GAACCGCTGA TGCTGAACCA CCAGCCTGAG AGCCTGCAGG ATGCGGAAAG TGGAGACGGA 
GGGGAGGAGA ATATTGTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025000657.1
CDS	228..4994
Translation	MAIAHEELPSTWSILGSCYEGEPTHKETLLHLAMKLGLVKLSEFLVGQPGGTLALALPNEDGATPLDLAEQNGHSKLVEVFSNFQGSRSPDVARVEISKGACMSFVRSSAALTLTLDHTAKHLLESDVRLFRKYFWNRPLLHECINSNQLEPVEDPVMRCSTTNSVEGPVSLAPDDLSQDGEKPVHGAVVPSKEVEDNVLLDIAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_025144889.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025144889.1

ORF Insert Sequence:

ATGGCCATTG CTCACGAAGA ATTGCCATCA ACGTGGAGCA TCCTCGGAAG CTGCTATGAA 
GGAGAGCCTA CACACAAAGA AACTCTTCTC CACCTTGCAA TGAAATTGGG CTTGGTTAAA 
CTTTCAGAGT TCTTAGTGGG CCAGCCTGGA GGGACATTGG CATTAGCGCT ACCTAATGAG 
GATGGAGCAA CACCATTAGA CTTGGCTGAG CAAAACGGGC ACTCCAAACT AGTTGAAGTC 
TTCTCAAATT TCCAGGGCAG CCGCTCGCCT GACGTTGCCA GAGTGGAGAT CAGCAAGGGG 
GCCTGCATGA GCTTTGTGCG CTCGTCAGCA GCTCTGACGC TGACGCTGGA CCACACGGCC 
AAGCACTTAC TGGAGTCAGA TGTGAGGCTC TTCAGGAAGT ACTTCTGGAA CAGGCCGCTG 
CTCCATGAGT GTATCAATTC AAACCAGCTT GAGCCAGTGG AAGATCCAGT AATGCGCTGC 
AGCACAACAA ACTCTGTAGA AGGTCCTGTG AGCTTGGCAC CTGATGACTT GTCCCAGGAT 
GGTGAGAAGC CTGTCCATGG TGCTGTGGTT CCTTCTAAAG AAGTTGAAGA TAATGTCCTG 
CTAGACATAG CACAGAGACA TTCTACCCTT GATATTCTTA AGAAGCTGAA AACTCCACCT 
ACCTTCTTTG CTGCAGCCAG ACTTTCTACT ATGCTGAATG GAAGTGATGA AGTCTATGCG 
AACTGCATGC TAGTAGACCA GGCTGAAGAT TTAAAAATGA ACTACATTCA TGGAGATAGC 
ATTAGCAGTG AAACGTGTCT GAATCCCAGC AGCTCAGCCC CAGTAGCGAA GAGCTCTTCG 
TCTCCTTCCC TTGAGGAGAA TGACCAGTAT AGATGCGATC CAAGTGAAGG AAATCAGTCA 
GTGAAAAATG AAGGAAATAC AGGCTTAAGA CCTACTGTTT CCCCATCTGA TCTGTATGAA 
GCAGATTCCT ACCTTCCATT CAAGACAAAT GAGTTTATTA AGGAACAGGG CCAACTTCAT 
GCTGTTATGA AAAAAAAAAG TTCAAGTCTC GATGACCTTG AAGTAAACAG CAGAAGTGAA 
GGTGTCTTGG GCAGATCCAA TATACCCTAC CCTCTTCTTG AACATCCAGA GGTGAAGGCT 
TGTAGCAGAG ATGGATTAGA TTCTTCTGAG ATGAACATCA GCCGGACAGA ATCACTGCCC 
TTATCTATCA GTCCACAGAC CAAGGAATGT TCACCCTCTG GAATTCGGTC ACGCTCGTAT 
TCCTGTTCCT CACCCAAAGA CTCATTGGGA AGACCTCGCA TTCTTCAGGA CTTCACAGTT 
GGTGATTCAG GTGAAGATGG TGTTCCCATG AACAGTGGCC GATCCCTCCT ACAGGCTCTC 
TCACTCTCTA AGTCAGTGTC CCTGCTCCAC CCCTGTAAAC GAGCATACAG CTTGCCTGAG 
CACTCCCGAG AGAAAAGGAT TCAGGAGGAA GAATGGGATA AGTATATTGT ACCTTCAAAA 
ACAGAGTCTG AAAAATACAA AGTGAGTCGG ACTTTCAGCT TTCTGATGAA TAGAATGACT 
AGCACACGGA ATAAGTACAA GACAAAAAAT AAAGATACCA AAGACAAAGA GAAGCTGAAC 
AGACATAATT TTATAAATGG AACCTTCTCT GGAATTATGC CATGTATGGC CTGTGAAAAG 
GCTCTCCTAG GAAAAGAGTC ACTGCAGTGC TCTTATTGCA ATGTGATTGT GCATAAGAAC 
TGTAAGGAGT GTGTTCCTGT GTGCACTAAG AAATCCCAGG AAAGGTACCA TAGCAAAAAC 
AAACCTCAAG TTGGCATAGG AAATTTATCG TTTGGAGACA TTTCACAGCC TGGGATTTCC 
TCAGTGCATC CTTCGTCCTC TGTACCCATT GTATCTGCTG GGAGGAGGGA AGCCTTGCAG 
CAGTCCCATT CCTTGTCCAA AAGTGTCCCT GGTGCCAGTT TTGAAAGAAG ATCTATGCCG 
TTTCCTGAGC AGGACTCTGA TGCTAGCACA TGGAGGACCA AGTCCAGATC TGAGGAGATG 
TTACAGGCGT TAGGGACATA TTCTTCAATG GACTCTTTTG TGATGGAAGT TGAGGTGGAT 
TCGTCTCAGT GGACTGACAT CAGTTCAGAT GCACACGAGT TTGAGGCGGA GTCATGGAGT 
CTTGTTGTGG ATCCGATGTT CTGTAACAAG CAAGAAAAGG ATGTCGCCAA GAGGCAGGAT 
GTCATTTTTG AGCTGATGCA AACAGAAGTT CATCACATCC ACACCCTGTT CATTATGTCT 
GAAATATTCA GGAAGGGAAT GAAAGAAGAA CTGCAGCTGG ACCACAGCAC GGTGGACAGA 
ATATTCCCCT GCTTAGATGA GCTACTGGAG ATCCATAGGC AGTTCTTTTG TAGAATGAGG 
GAGAGACGGC AGGAATCGTG TGAGGAGGGG AATGAGCGTA ATTTTGTAAT CAGCAGAATC 
GGAGATATCC TTGTGCAACA GTTTTCAGAG GAAAATGCAA CTAAAATGAA GAAAATATAT 
GGAGAATTTT GCAGCCATCA GAAAGAAGCT GTTAATCTCT TCAAAGAGTT GCAGCAAAAC 
AAGAAGTTCC AGAATTTTAT TAAACTGAGG AACAGCAATT TGTTGGCACG CCGCAGAGGA 
ATCCCCGAAT GCATTCTGCT TGTCACTCAG CGAATCACCA AATACCCTGT TCTAGTTGAA 
AGGATACTGC AGTACACAAA AGAAGGAACA GAAGAACATA AAGACCTATG CAAAGCCCTT 
CGTTTAATTA AAGACATGAT TGCAGCAGTA GATTTGAAAG TTAATGAATA CGAGAAAAGG 
CAAAAACTGA TGGAAATTCT CAACCGAACT GAAAACAAAA CATATACAAA ACTGAAAAGT 
GGACACATTT TTAGGAAGCA AGACTTGATG AGGAAAGAGA GGATACTTCT TCATGAAGGT 
TTAGTGTACT GGAAAACAGC TACTGGTCGT TTCAAAGACA CCCTAGCTCT GCTTCTAACT 
GATATGCTGC TGTTCTTGCA AGAAAAAGAT CAGAAGTACG TTTTTGCAGC TGTTGACCAG 
AAGCCTTCAG TAATCTCTCT TCAGAAGCTC ATTGTAAGAG AAGTAGCCAA TGAAGAAAGG 
GGGATGTTTC TGATTAGTGC TTCATCTGCA GGACCTGAGA TGTATGAAGT TCATACGAAC 
TCCAGAGAAG AGCGTAACAG CTGGATGAGG CATATTCAAG ATGCAGTGGA AAGTTGTCCA 
GAGGAACAGG AAGAAGGAAA AATAGGTGAA TTTGATGAGG ACAGACGTAT CGCTGAGGCT 
AAAGCATGCA AAATTCAGAA ATGTCAAGAA TCACTGAGTA ACCAGGACCA GCAAATCTGC 
AGTTGCTTGG AAGACAAATT GCATATCTAT GCAGAACTAG TAGATATGAG TGGATTTGAA 
GATGTTCATG TAGAACCTCA CCTCCTTATC AAACCTGATT ATGGAGAAAC TCCACAGGCT 
TCTTCGTTGC TGGTAGCAGC CCTCAGAGAA GTTGAAAGTC TCCACGTTGC TCTCACGTCA 
TCACAAGTGA ATGAAACGGA CAGATCATCA GAGGAAAGTA CAGAGGAATT AACTTTGAAA 
CACAGACTTA GTGCCAGTGA AATCTTGGAA TCTTTCCCTG GTGATGCAGA AACAAAGGAA 
GTTGAAGAAT CACCTGAAGT TGATCTGAGT GCTTGCTCTA TTGAAAAGGA AATTTCTGAT 
GCCAGTGTAG AGTATAAGGG AGAAAGTACG AGTTTCCCAG GATCTACAGG GACAGAGATT 
GTACAAGCAA TCCAGAATCT AACTCGTCTC TTGTACAGCA TACAGGCAGC GTTAGCTATC 
CAGGACAGCC ACAGAGAGCT CCATAGGTTA CTCCTGCAAG AGAATGAGAA GACCAGTCGT 
GGCCATAGTT CTCGGATACC CCTCTTCCTG GAACCAGAAA AATACCGTAA TTCAGAAAAA 
CAAAGGGAGG AGCTGGCAAA CATCAACAAA CTCCAGCAGC AGTTCCAGCA AGAGCAGCAG 
CGCTGGCTGC GTGAATGCGA GCAGCGGCAG CAAGAGCTGG AGGTAAGGGC AGGCCTGCTG 
GAGCAGAGGG AGCAGGCCTG CAGCTGCCAG GAGGAGCTGC TGGAGAAGAG CCGCCAGGGG 
CTTGCACTGC AGTCGCAGGA GTTCCAGCAG AGCCTGGAGA GGCTGCGGGA AGGGCAGAAG 
TTGTTGGAGA AAAAAAGGGA CAGTATCAAA ATGCAGAAGA AGCTCCTCTG GCACTGGAAG 
CATGGTCTCC AAAGCAGCCT GCTGCTGCCC ACAGGGAGCC ACGAGATAAT GGGACACGAT 
CAGTTGGATA GCTTACATGG AGAAAATGCG TTCTATTTAA ATGAAGCTGT AGTACGCGTG 
TCTCTAAGCG GCCTCAACAG ATCAGATTCT TCTCTTGTTT ATGAAGATGG TGTGTATGGG 
CTGGGCATTT CAGACTTGGA TATGGCAAGG ACTTGTGAAA ATCAGGTGAA CCTCAGGGTG 
GATACTCCTT GCCAACCAGC ATTGACCAAT GAACTGTGGA AATCCACTGG CCCTTACCAG 
CAGATGTCTT TCTCCTGCAA AAGCAACAAG GATGCTTTTA ATAATGATCT AAATGCATCA 
CAGACCCAGG GTCCCCAGGC AGGCAGTCCA AACCATGGAT CCATTCAGCC ACCACAAGTG 
GAACCGCTGA TGCTGAACCA CCAGCCTGAG AGCCTGCAGG ATGCGGAAAG TGGAGACGGA 
GGGGAGGAGA ATATTGTTTA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_015277657.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133143.1 Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

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CloneID	OGa47913
Clone ID Related Accession (Same CDS sequence)	XM_015277657.2 , XM_015277657.1
Accession Version	XM_015277657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015277657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG 
CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA 
GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT 
TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA 
GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT 
GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA 
CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC 
AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC 
CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT 
CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG 
AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT 
ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA 
TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA 
AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC 
AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA 
GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG 
ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA 
GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT 
GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG 
TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG 
ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC 
AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC 
AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT 
GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG 
TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT 
GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA 
GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC 
TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC 
AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT 
GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA 
TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC 
ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG 
TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG 
CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA 
ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT 
GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG 
TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA 
CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT 
CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT 
ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG 
CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA 
AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG 
ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT 
CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG 
CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA 
GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG 
CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT 
CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT 
GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC 
AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA 
AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG 
AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA 
TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT 
CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA 
GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG 
GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT 
GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA 
GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG 
GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT 
CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG 
CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA 
CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA 
AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC 
GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC 
TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG 
GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG 
GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC 
CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT 
GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC 
AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG 
GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA 
GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC 
AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG 
GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC 
CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT 
TACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133143.1
CDS	1568..6136
Translation	MASVTGRSTITNSNEESFQGSRSPDVARVEISKGACMSFVRSSAALTLTLDHTAKHLLESDVRLFRKYFWNRPLLHECINSNQLEPVEDPVMRCSTTNSVEGPVSLAPDDLSQDGEKPVHGAVVPSKEVEDNVLLDIAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277657.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277657.2

ORF Insert Sequence:

ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG 
CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA 
GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT 
TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA 
GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT 
GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA 
CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC 
AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC 
CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT 
CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG 
AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT 
ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA 
TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA 
AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC 
AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA 
GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG 
ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA 
GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT 
GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG 
TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG 
ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC 
AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC 
AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT 
GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG 
TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT 
GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA 
GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC 
TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC 
AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT 
GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA 
TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC 
ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG 
TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG 
CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA 
ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT 
GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG 
TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA 
CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT 
CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT 
ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG 
CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA 
AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG 
ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT 
CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG 
CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA 
GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG 
CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT 
CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT 
GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC 
AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA 
AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG 
AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA 
TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT 
CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA 
GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG 
GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT 
GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA 
GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG 
GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT 
CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG 
CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA 
CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA 
AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC 
GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC 
TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG 
GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG 
GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC 
CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT 
GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC 
AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG 
GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA 
GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC 
AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG 
GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC 
CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT 
TACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_015277657.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133143.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X2, mRNA.

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CloneID	OGa47913
Clone ID Related Accession (Same CDS sequence)	XM_015277657.2 , XM_015277657.1
Accession Version	XM_015277657.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG 
CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA 
GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT 
TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA 
GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT 
GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA 
CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC 
AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC 
CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT 
CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG 
AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT 
ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA 
TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA 
AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC 
AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA 
GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG 
ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA 
GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT 
GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG 
TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG 
ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC 
AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC 
AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT 
GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG 
TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT 
GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA 
GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC 
TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC 
AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT 
GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA 
TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC 
ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG 
TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG 
CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA 
ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT 
GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG 
TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA 
CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT 
CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT 
ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG 
CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA 
AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG 
ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT 
CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG 
CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA 
GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG 
CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT 
CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT 
GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC 
AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA 
AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG 
AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA 
TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT 
CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA 
GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG 
GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT 
GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA 
GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG 
GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT 
CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG 
CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA 
CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA 
AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC 
GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC 
TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG 
GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG 
GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC 
CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT 
GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC 
AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG 
GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA 
GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC 
AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG 
GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC 
CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT 
TACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133143.1
CDS	36..4604
Translation	MASVTGRSTITNSNEESFQGSRSPDVARVEISKGACMSFVRSSAALTLTLDHTAKHLLESDVRLFRKYFWNRPLLHECINSNQLEPVEDPVMRCSTTNSVEGPVSLAPDDLSQDGEKPVHGAVVPSKEVEDNVLLDIAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277657.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277657.1

ORF Insert Sequence:

ATGGCATCAG TAACAGGAAG AAGTACTATT ACTAACAGCA ATGAGGAGAG TTTCCAGGGC 
AGCCGCTCGC CTGACGTTGC CAGAGTGGAG ATCAGCAAGG GGGCCTGCAT GAGCTTTGTG 
CGCTCGTCAG CAGCTCTGAC GCTGACGCTG GACCACACGG CCAAGCACTT ACTGGAGTCA 
GATGTGAGGC TCTTCAGGAA GTACTTCTGG AACAGGCCGC TGCTCCATGA GTGTATCAAT 
TCAAACCAGC TTGAGCCAGT GGAAGATCCA GTAATGCGCT GCAGCACAAC AAACTCTGTA 
GAAGGTCCTG TGAGCTTGGC ACCTGATGAC TTGTCCCAGG ATGGTGAGAA GCCTGTCCAT 
GGTGCTGTGG TTCCTTCTAA AGAAGTTGAA GATAATGTCC TGCTAGACAT AGCACAGAGA 
CATTCTACCC TTGATATTCT TAAGAAGCTG AAAACTCCAC CTACCTTCTT TGCTGCAGCC 
AGACTTTCTA CTATGCTGAA TGGAAGTGAT GAAGTCTATG CGAACTGCAT GCTAGTAGAC 
CAGGCTGAAG ATTTAAAAAT GAACTACATT CATGGAGATA GCATTAGCAG TGAAACGTGT 
CTGAATCCCA GCAGCTCAGC CCCAGTAGCG AAGAGCTCTT CGTCTCCTTC CCTTGAGGAG 
AATGACCAGT ATAGATGCGA TCCAAGTGAA GGAAATCAGT CAGTGAAAAA TGAAGGAAAT 
ACAGGCTTAA GACCTACTGT TTCCCCATCT GATCTGTATG AAGCAGATTC CTACCTTCCA 
TTCAAGACAA ATGAGTTTAT TAAGGAACAG GGCCAACTTC ATGCTGTTAT GAAAAAAAAA 
AGTTCAAGTC TCGATGACCT TGAAGTAAAC AGCAGAAGTG AAGGTGTCTT GGGCAGATCC 
AATATACCCT ACCCTCTTCT TGAACATCCA GAGGTGAAGG CTTGTAGCAG AGATGGATTA 
GATTCTTCTG AGATGAACAT CAGCCGGACA GAATCACTGC CCTTATCTAT CAGTCCACAG 
ACCAAGGAAT GTTCACCCTC TGGAATTCGG TCACGCTCGT ATTCCTGTTC CTCACCCAAA 
GACTCATTGG GAAGACCTCG CATTCTTCAG GACTTCACAG TTGGTGATTC AGGTGAAGAT 
GGTGTTCCCA TGAACAGTGG CCGATCCCTC CTACAGGCTC TCTCACTCTC TAAGTCAGTG 
TCCCTGCTCC ACCCCTGTAA ACGAGCATAC AGCTTGCCTG AGCACTCCCG AGAGAAAAGG 
ATTCAGGAGG AAGAATGGGA TAAGTATATT GTACCTTCAA AAACAGAGTC TGAAAAATAC 
AAAGTGAGTC GGACTTTCAG CTTTCTGATG AATAGAATGA CTAGCACACG GAATAAGTAC 
AAGACAAAAA ATAAAGATAC CAAAGACAAA GAGAAGCTGA ACAGACATAA TTTTATAAAT 
GGAACCTTCT CTGGAATTAT GCCATGTATG GCCTGTGAAA AGGCTCTCCT AGGAAAAGAG 
TCACTGCAGT GCTCTTATTG CAATGTGATT GTGCATAAGA ACTGTAAGGA GTGTGTTCCT 
GTGTGCACTA AGAAATCCCA GGAAAGGTAC CATAGCAAAA ACAAACCTCA AGTTGGCATA 
GGAAATTTAT CGTTTGGAGA CATTTCACAG CCTGGGATTT CCTCAGTGCA TCCTTCGTCC 
TCTGTACCCA TTGTATCTGC TGGGAGGAGG GAAGCCTTGC AGCAGTCCCA TTCCTTGTCC 
AAAAGTGTCC CTGGTGCCAG TTTTGAAAGA AGATCTATGC CGTTTCCTGA GCAGGACTCT 
GATGCTAGCA CATGGAGGAC CAAGTCCAGA TCTGAGGAGA TGTTACAGGC GTTAGGGACA 
TATTCTTCAA TGGACTCTTT TGTGATGGAA GTTGAGGTGG ATTCGTCTCA GTGGACTGAC 
ATCAGTTCAG ATGCACACGA GTTTGAGGCG GAGTCATGGA GTCTTGTTGT GGATCCGATG 
TTCTGTAACA AGCAAGAAAA GGATGTCGCC AAGAGGCAGG ATGTCATTTT TGAGCTGATG 
CAAACAGAAG TTCATCACAT CCACACCCTG TTCATTATGT CTGAAATATT CAGGAAGGGA 
ATGAAAGAAG AACTGCAGCT GGACCACAGC ACGGTGGACA GAATATTCCC CTGCTTAGAT 
GAGCTACTGG AGATCCATAG GCAGTTCTTT TGTAGAATGA GGGAGAGACG GCAGGAATCG 
TGTGAGGAGG GGAATGAGCG TAATTTTGTA ATCAGCAGAA TCGGAGATAT CCTTGTGCAA 
CAGTTTTCAG AGGAAAATGC AACTAAAATG AAGAAAATAT ATGGAGAATT TTGCAGCCAT 
CAGAAAGAAG CTGTTAATCT CTTCAAAGAG TTGCAGCAAA ACAAGAAGTT CCAGAATTTT 
ATTAAACTGA GGAACAGCAA TTTGTTGGCA CGCCGCAGAG GAATCCCCGA ATGCATTCTG 
CTTGTCACTC AGCGAATCAC CAAATACCCT GTTCTAGTTG AAAGGATACT GCAGTACACA 
AAAGAAGGAA CAGAAGAACA TAAAGACCTA TGCAAAGCCC TTCGTTTAAT TAAAGACATG 
ATTGCAGCAG TAGATTTGAA AGTTAATGAA TACGAGAAAA GGCAAAAACT GATGGAAATT 
CTCAACCGAA CTGAAAACAA AACATATACA AAACTGAAAA GTGGACACAT TTTTAGGAAG 
CAAGACTTGA TGAGGAAAGA GAGGATACTT CTTCATGAAG GTTTAGTGTA CTGGAAAACA 
GCTACTGGTC GTTTCAAAGA CACCCTAGCT CTGCTTCTAA CTGATATGCT GCTGTTCTTG 
CAAGAAAAAG ATCAGAAGTA CGTTTTTGCA GCTGTTGACC AGAAGCCTTC AGTAATCTCT 
CTTCAGAAGC TCATTGTAAG AGAAGTAGCC AATGAAGAAA GGGGGATGTT TCTGATTAGT 
GCTTCATCTG CAGGACCTGA GATGTATGAA GTTCATACGA ACTCCAGAGA AGAGCGTAAC 
AGCTGGATGA GGCATATTCA AGATGCAGTG GAAAGTTGTC CAGAGGAACA GGAAGAAGGA 
AAAATAGGTG AATTTGATGA GGACAGACGT ATCGCTGAGG CTAAAGCATG CAAAATTCAG 
AAATGTCAAG AATCACTGAG TAACCAGGAC CAGCAAATCT GCAGTTGCTT GGAAGACAAA 
TTGCATATCT ATGCAGAACT AGTAGATATG AGTGGATTTG AAGATGTTCA TGTAGAACCT 
CACCTCCTTA TCAAACCTGA TTATGGAGAA ACTCCACAGG CTTCTTCGTT GCTGGTAGCA 
GCCCTCAGAG AAGTTGAAAG TCTCCACGTT GCTCTCACGT CATCACAAGT GAATGAAACG 
GACAGATCAT CAGAGGAAAG TACAGAGGAA TTAACTTTGA AACACAGACT TAGTGCCAGT 
GAAATCTTGG AATCTTTCCC TGGTGATGCA GAAACAAAGG AAGTTGAAGA ATCACCTGAA 
GTTGATCTGA GTGCTTGCTC TATTGAAAAG GAAATTTCTG ATGCCAGTGT AGAGTATAAG 
GGAGAAAGTA CGAGTTTCCC AGGATCTACA GGGACAGAGA TTGTACAAGC AATCCAGAAT 
CTAACTCGTC TCTTGTACAG CATACAGGCA GCGTTAGCTA TCCAGGACAG CCACAGAGAG 
CTCCATAGGT TACTCCTGCA AGAGAATGAG AAGACCAGTC GTGGCCATAG TTCTCGGATA 
CCCCTCTTCC TGGAACCAGA AAAATACCGT AATTCAGAAA AACAAAGGGA GGAGCTGGCA 
AACATCAACA AACTCCAGCA GCAGTTCCAG CAAGAGCAGC AGCGCTGGCT GCGTGAATGC 
GAGCAGCGGC AGCAAGAGCT GGAGGTAAGG GCAGGCCTGC TGGAGCAGAG GGAGCAGGCC 
TGCAGCTGCC AGGAGGAGCT GCTGGAGAAG AGCCGCCAGG GGCTTGCACT GCAGTCGCAG 
GAGTTCCAGC AGAGCCTGGA GAGGCTGCGG GAAGGGCAGA AGTTGTTGGA GAAAAAAAGG 
GACAGTATCA AAATGCAGAA GAAGCTCCTC TGGCACTGGA AGCATGGTCT CCAAAGCAGC 
CTGCTGCTGC CCACAGGGAG CCACGAGATA ATGGGACACG ATCAGTTGGA TAGCTTACAT 
GGAGAAAATG CGTTCTATTT AAATGAAGCT GTAGTACGCG TGTCTCTAAG CGGCCTCAAC 
AGATCAGATT CTTCTCTTGT TTATGAAGAT GGTGTGTATG GGCTGGGCAT TTCAGACTTG 
GATATGGCAA GGACTTGTGA AAATCAGGTG AACCTCAGGG TGGATACTCC TTGCCAACCA 
GCATTGACCA ATGAACTGTG GAAATCCACT GGCCCTTACC AGCAGATGTC TTTCTCCTGC 
AAAAGCAACA AGGATGCTTT TAATAATGAT CTAAATGCAT CACAGACCCA GGGTCCCCAG 
GCAGGCAGTC CAAACCATGG ATCCATTCAG CCACCACAAG TGGAACCGCT GATGCTGAAC 
CACCAGCCTG AGAGCCTGCA GGATGCGGAA AGTGGAGACG GAGGGGAGGA GAATATTGTT 
TACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_025144888.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025000656.1 Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

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CloneID	OGa21189
Clone ID Related Accession (Same CDS sequence)	XM_025144888.1 , XM_015277655.1
Accession Version	XM_025144888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006127.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG 
AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA 
GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA 
CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT 
CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA 
AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT 
GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT 
ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG 
TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA 
ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT 
TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG 
AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA 
CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG 
TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA 
AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG 
CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA 
GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT 
GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG 
CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT 
GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC 
CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT 
GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC 
TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG 
AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG 
GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA 
GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC 
AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC 
TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA 
GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT 
AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA 
GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT 
GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG 
AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT 
TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA 
GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG 
TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA 
GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT 
CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT 
TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG 
CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG 
TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG 
TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG 
GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT 
GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC 
AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC 
TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG 
CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC 
CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT 
TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC 
CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA 
TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG 
CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT 
AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG 
ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT 
TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC 
TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG 
CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA 
GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT 
CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA 
GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG 
GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC 
AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG 
GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT 
GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG 
CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG 
AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT 
AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA 
TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA 
GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA 
ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC 
CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT 
TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG 
GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG 
CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG 
GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG 
CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG 
AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC 
CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT 
AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC 
GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT 
TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT 
TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT 
TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG 
GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG 
ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG 
AATATTGTTT ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025000656.1
CDS	153..5330
Translation	MELSCTEVPLYGQVTISAKFDKGIYLPEDAEFYFVYDGSMKRHIMFAERIGDNALQSLVPGHGCQEAVSVSVLMYSRGSSPVALGSCPVTYRENLSCKLSRFLISHTECISTTSHKVLLEKFGLTVKDLQSLDTDLTMAIAHEELPSTWSILGSCYEGEPTHKETLLHLAMKLGLVKLSEFLVGQPGGTLALALPNEDGATPLDLAEQNGHSKLVEVFSNFQGSRSPDVARVEISKGACMSFVRSSAALTLTLDHTAKHLLESDVRLFRKYFWNRPLLHECINSNQLEPVEDPVMRCSTTNSVEGPVSLAPDDLSQDGEKPVHGAVVPSKEVEDNVLLDIAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_025144888.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025144888.1

ORF Insert Sequence:

ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG 
AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA 
GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA 
CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT 
CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA 
AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT 
GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT 
ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG 
TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA 
ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT 
TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG 
AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA 
CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG 
TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA 
AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG 
CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA 
GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT 
GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG 
CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT 
GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC 
CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT 
GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC 
TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG 
AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG 
GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA 
GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC 
AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC 
TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA 
GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT 
AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA 
GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT 
GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG 
AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT 
TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA 
GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG 
TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA 
GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT 
CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT 
TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG 
CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG 
TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG 
TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG 
GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT 
GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC 
AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC 
TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG 
CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC 
CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT 
TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC 
CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA 
TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG 
CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT 
AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG 
ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT 
TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC 
TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG 
CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA 
GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT 
CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA 
GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG 
GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC 
AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG 
GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT 
GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG 
CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG 
AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT 
AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA 
TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA 
GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA 
ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC 
CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT 
TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG 
GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG 
CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG 
GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG 
CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG 
AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC 
CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT 
AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC 
GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT 
TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT 
TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT 
TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG 
GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG 
ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG 
AATATTGTTT ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC427201 ( XM_015277655.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015133141.1 Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X1, mRNA.

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CloneID	OGa21189
Clone ID Related Accession (Same CDS sequence)	XM_025144888.1 , XM_015277655.1
Accession Version	XM_015277655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_001488830.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG 
AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA 
GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA 
CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT 
CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA 
AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT 
GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT 
ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG 
TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA 
ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT 
TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG 
AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA 
CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG 
TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA 
AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG 
CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA 
GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT 
GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG 
CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT 
GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC 
CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT 
GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC 
TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG 
AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG 
GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA 
GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC 
AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC 
TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA 
GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT 
AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA 
GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT 
GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG 
AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT 
TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA 
GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG 
TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA 
GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT 
CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT 
TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG 
CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG 
TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG 
TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG 
GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT 
GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC 
AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC 
TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG 
CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC 
CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT 
TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC 
CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA 
TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG 
CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT 
AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG 
ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT 
TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC 
TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG 
CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA 
GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT 
CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA 
GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG 
GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC 
AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG 
GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT 
GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG 
CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG 
AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT 
AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA 
TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA 
GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA 
ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC 
CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT 
TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG 
GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG 
CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG 
GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG 
CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG 
AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC 
CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT 
AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC 
GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT 
TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT 
TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT 
TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG 
GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG 
ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG 
AATATTGTTT ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015133141.1
CDS	364..5541
Translation	MELSCTEVPLYGQVTISAKFDKGIYLPEDAEFYFVYDGSMKRHIMFAERIGDNALQSLVPGHGCQEAVSVSVLMYSRGSSPVALGSCPVTYRENLSCKLSRFLISHTECISTTSHKVLLEKFGLTVKDLQSLDTDLTMAIAHEELPSTWSILGSCYEGEPTHKETLLHLAMKLGLVKLSEFLVGQPGGTLALALPNEDGATPLDLAEQNGHSKLVEVFSNFQGSRSPDVARVEISKGACMSFVRSSAALTLTLDHTAKHLLESDVRLFRKYFWNRPLLHECINSNQLEPVEDPVMRCSTTNSVEGPVSLAPDDLSQDGEKPVHGAVVPSKEVEDNVLLDIAQRHSTLDILKKLKTPPTFFAAARLSTMLNGSDEVYANCMLVDQAEDLKMNYIHGDSISSETCLNPSSSAPVAKSSSSPSLEENDQYRCDPSEGNQSVKNEGNTGLRPTVSPSDLYEADSYLPFKTNEFIKEQGQLHAVMKKKSSSLDDLEVNSRSEGVLGRSNIPYPLLEHPEVKACSRDGLDSSEMNISRTESLPLSISPQTKECSPSGIRSRSYSCSSPKDSLGRPRILQDFTVGDSGEDGVPMNSGRSLLQALSLSKSVSLLHPCKRAYSLPEHSREKRIQEEEWDKYIVPSKTESEKYKVSRTFSFLMNRMTSTRNKYKTKNKDTKDKEKLNRHNFINGTFSGIMPCMACEKALLGKESLQCSYCNVIVHKNCKECVPVCTKKSQERYHSKNKPQVGIGNLSFGDISQPGISSVHPSSSVPIVSAGRREALQQSHSLSKSVPGASFERRSMPFPEQDSDASTWRTKSRSEEMLQALGTYSSMDSFVMEVEVDSSQWTDISSDAHEFEAESWSLVVDPMFCNKQEKDVAKRQDVIFELMQTEVHHIHTLFIMSEIFRKGMKEELQLDHSTVDRIFPCLDELLEIHRQFFCRMRERRQESCEEGNERNFVISRIGDILVQQFSEENATKMKKIYGEFCSHQKEAVNLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKEGTEEHKDLCKALRLIKDMIAAVDLKVNEYEKRQKLMEILNRTENKTYTKLKSGHIFRKQDLMRKERILLHEGLVYWKTATGRFKDTLALLLTDMLLFLQEKDQKYVFAAVDQKPSVISLQKLIVREVANEERGMFLISASSAGPEMYEVHTNSREERNSWMRHIQDAVESCPEEQEEGKIGEFDEDRRIAEAKACKIQKCQESLSNQDQQICSCLEDKLHIYAELVDMSGFEDVHVEPHLLIKPDYGETPQASSLLVAALREVESLHVALTSSQVNETDRSSEESTEELTLKHRLSASEILESFPGDAETKEVEESPEVDLSACSIEKEISDASVEYKGESTSFPGSTGTEIVQAIQNLTRLLYSIQAALAIQDSHRELHRLLLQENEKTSRGHSSRIPLFLEPEKYRNSEKQREELANINKLQQQFQQEQQRWLRECEQRQQELEVRAGLLEQREQACSCQEELLEKSRQGLALQSQEFQQSLERLREGQKLLEKKRDSIKMQKKLLWHWKHGLQSSLLLPTGSHEIMGHDQLDSLHGENAFYLNEAVVRVSLSGLNRSDSSLVYEDGVYGLGISDLDMARTCENQVNLRVDTPCQPALTNELWKSTGPYQQMSFSCKSNKDAFNNDLNASQTQGPQAGSPNHGSIQPPQVEPLMLNHQPESLQDAESGDGGEENIVYL

Target ORF information:

RefSeq Version	XM_015277655.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus rho-guanine nucleotide exchange factor-like (LOC427201), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015277655.1

ORF Insert Sequence:

ATGGAGCTAA GCTGCACGGA AGTTCCTCTT TATGGACAGG TGACTATAAG TGCAAAATTT 
GACAAAGGCA TTTACCTCCC GGAAGATGCT GAGTTCTACT TTGTTTATGA TGGATCCATG 
AAGAGGCACA TAATGTTTGC TGAGCGTATT GGTGACAATG CTCTTCAGTC CCTTGTTCCA 
GGCCATGGAT GCCAGGAAGC AGTCTCTGTC TCTGTGTTAA TGTATTCAAG GGGATCCTCA 
CCTGTGGCCC TTGGCAGCTG TCCTGTCACT TACAGGGAGA ACTTGTCTTG CAAGCTGTCT 
CGTTTTCTGA TCAGTCACAC AGAGTGCATC AGTACCACAA GTCACAAGGT GTTACTGGAA 
AAGTTTGGAC TAACAGTAAA AGATCTTCAG TCCCTGGATA CTGATTTGAC AATGGCCATT 
GCTCACGAAG AATTGCCATC AACGTGGAGC ATCCTCGGAA GCTGCTATGA AGGAGAGCCT 
ACACACAAAG AAACTCTTCT CCACCTTGCA ATGAAATTGG GCTTGGTTAA ACTTTCAGAG 
TTCTTAGTGG GCCAGCCTGG AGGGACATTG GCATTAGCGC TACCTAATGA GGATGGAGCA 
ACACCATTAG ACTTGGCTGA GCAAAACGGG CACTCCAAAC TAGTTGAAGT CTTCTCAAAT 
TTCCAGGGCA GCCGCTCGCC TGACGTTGCC AGAGTGGAGA TCAGCAAGGG GGCCTGCATG 
AGCTTTGTGC GCTCGTCAGC AGCTCTGACG CTGACGCTGG ACCACACGGC CAAGCACTTA 
CTGGAGTCAG ATGTGAGGCT CTTCAGGAAG TACTTCTGGA ACAGGCCGCT GCTCCATGAG 
TGTATCAATT CAAACCAGCT TGAGCCAGTG GAAGATCCAG TAATGCGCTG CAGCACAACA 
AACTCTGTAG AAGGTCCTGT GAGCTTGGCA CCTGATGACT TGTCCCAGGA TGGTGAGAAG 
CCTGTCCATG GTGCTGTGGT TCCTTCTAAA GAAGTTGAAG ATAATGTCCT GCTAGACATA 
GCACAGAGAC ATTCTACCCT TGATATTCTT AAGAAGCTGA AAACTCCACC TACCTTCTTT 
GCTGCAGCCA GACTTTCTAC TATGCTGAAT GGAAGTGATG AAGTCTATGC GAACTGCATG 
CTAGTAGACC AGGCTGAAGA TTTAAAAATG AACTACATTC ATGGAGATAG CATTAGCAGT 
GAAACGTGTC TGAATCCCAG CAGCTCAGCC CCAGTAGCGA AGAGCTCTTC GTCTCCTTCC 
CTTGAGGAGA ATGACCAGTA TAGATGCGAT CCAAGTGAAG GAAATCAGTC AGTGAAAAAT 
GAAGGAAATA CAGGCTTAAG ACCTACTGTT TCCCCATCTG ATCTGTATGA AGCAGATTCC 
TACCTTCCAT TCAAGACAAA TGAGTTTATT AAGGAACAGG GCCAACTTCA TGCTGTTATG 
AAAAAAAAAA GTTCAAGTCT CGATGACCTT GAAGTAAACA GCAGAAGTGA AGGTGTCTTG 
GGCAGATCCA ATATACCCTA CCCTCTTCTT GAACATCCAG AGGTGAAGGC TTGTAGCAGA 
GATGGATTAG ATTCTTCTGA GATGAACATC AGCCGGACAG AATCACTGCC CTTATCTATC 
AGTCCACAGA CCAAGGAATG TTCACCCTCT GGAATTCGGT CACGCTCGTA TTCCTGTTCC 
TCACCCAAAG ACTCATTGGG AAGACCTCGC ATTCTTCAGG ACTTCACAGT TGGTGATTCA 
GGTGAAGATG GTGTTCCCAT GAACAGTGGC CGATCCCTCC TACAGGCTCT CTCACTCTCT 
AAGTCAGTGT CCCTGCTCCA CCCCTGTAAA CGAGCATACA GCTTGCCTGA GCACTCCCGA 
GAGAAAAGGA TTCAGGAGGA AGAATGGGAT AAGTATATTG TACCTTCAAA AACAGAGTCT 
GAAAAATACA AAGTGAGTCG GACTTTCAGC TTTCTGATGA ATAGAATGAC TAGCACACGG 
AATAAGTACA AGACAAAAAA TAAAGATACC AAAGACAAAG AGAAGCTGAA CAGACATAAT 
TTTATAAATG GAACCTTCTC TGGAATTATG CCATGTATGG CCTGTGAAAA GGCTCTCCTA 
GGAAAAGAGT CACTGCAGTG CTCTTATTGC AATGTGATTG TGCATAAGAA CTGTAAGGAG 
TGTGTTCCTG TGTGCACTAA GAAATCCCAG GAAAGGTACC ATAGCAAAAA CAAACCTCAA 
GTTGGCATAG GAAATTTATC GTTTGGAGAC ATTTCACAGC CTGGGATTTC CTCAGTGCAT 
CCTTCGTCCT CTGTACCCAT TGTATCTGCT GGGAGGAGGG AAGCCTTGCA GCAGTCCCAT 
TCCTTGTCCA AAAGTGTCCC TGGTGCCAGT TTTGAAAGAA GATCTATGCC GTTTCCTGAG 
CAGGACTCTG ATGCTAGCAC ATGGAGGACC AAGTCCAGAT CTGAGGAGAT GTTACAGGCG 
TTAGGGACAT ATTCTTCAAT GGACTCTTTT GTGATGGAAG TTGAGGTGGA TTCGTCTCAG 
TGGACTGACA TCAGTTCAGA TGCACACGAG TTTGAGGCGG AGTCATGGAG TCTTGTTGTG 
GATCCGATGT TCTGTAACAA GCAAGAAAAG GATGTCGCCA AGAGGCAGGA TGTCATTTTT 
GAGCTGATGC AAACAGAAGT TCATCACATC CACACCCTGT TCATTATGTC TGAAATATTC 
AGGAAGGGAA TGAAAGAAGA ACTGCAGCTG GACCACAGCA CGGTGGACAG AATATTCCCC 
TGCTTAGATG AGCTACTGGA GATCCATAGG CAGTTCTTTT GTAGAATGAG GGAGAGACGG 
CAGGAATCGT GTGAGGAGGG GAATGAGCGT AATTTTGTAA TCAGCAGAAT CGGAGATATC 
CTTGTGCAAC AGTTTTCAGA GGAAAATGCA ACTAAAATGA AGAAAATATA TGGAGAATTT 
TGCAGCCATC AGAAAGAAGC TGTTAATCTC TTCAAAGAGT TGCAGCAAAA CAAGAAGTTC 
CAGAATTTTA TTAAACTGAG GAACAGCAAT TTGTTGGCAC GCCGCAGAGG AATCCCCGAA 
TGCATTCTGC TTGTCACTCA GCGAATCACC AAATACCCTG TTCTAGTTGA AAGGATACTG 
CAGTACACAA AAGAAGGAAC AGAAGAACAT AAAGACCTAT GCAAAGCCCT TCGTTTAATT 
AAAGACATGA TTGCAGCAGT AGATTTGAAA GTTAATGAAT ACGAGAAAAG GCAAAAACTG 
ATGGAAATTC TCAACCGAAC TGAAAACAAA ACATATACAA AACTGAAAAG TGGACACATT 
TTTAGGAAGC AAGACTTGAT GAGGAAAGAG AGGATACTTC TTCATGAAGG TTTAGTGTAC 
TGGAAAACAG CTACTGGTCG TTTCAAAGAC ACCCTAGCTC TGCTTCTAAC TGATATGCTG 
CTGTTCTTGC AAGAAAAAGA TCAGAAGTAC GTTTTTGCAG CTGTTGACCA GAAGCCTTCA 
GTAATCTCTC TTCAGAAGCT CATTGTAAGA GAAGTAGCCA ATGAAGAAAG GGGGATGTTT 
CTGATTAGTG CTTCATCTGC AGGACCTGAG ATGTATGAAG TTCATACGAA CTCCAGAGAA 
GAGCGTAACA GCTGGATGAG GCATATTCAA GATGCAGTGG AAAGTTGTCC AGAGGAACAG 
GAAGAAGGAA AAATAGGTGA ATTTGATGAG GACAGACGTA TCGCTGAGGC TAAAGCATGC 
AAAATTCAGA AATGTCAAGA ATCACTGAGT AACCAGGACC AGCAAATCTG CAGTTGCTTG 
GAAGACAAAT TGCATATCTA TGCAGAACTA GTAGATATGA GTGGATTTGA AGATGTTCAT 
GTAGAACCTC ACCTCCTTAT CAAACCTGAT TATGGAGAAA CTCCACAGGC TTCTTCGTTG 
CTGGTAGCAG CCCTCAGAGA AGTTGAAAGT CTCCACGTTG CTCTCACGTC ATCACAAGTG 
AATGAAACGG ACAGATCATC AGAGGAAAGT ACAGAGGAAT TAACTTTGAA ACACAGACTT 
AGTGCCAGTG AAATCTTGGA ATCTTTCCCT GGTGATGCAG AAACAAAGGA AGTTGAAGAA 
TCACCTGAAG TTGATCTGAG TGCTTGCTCT ATTGAAAAGG AAATTTCTGA TGCCAGTGTA 
GAGTATAAGG GAGAAAGTAC GAGTTTCCCA GGATCTACAG GGACAGAGAT TGTACAAGCA 
ATCCAGAATC TAACTCGTCT CTTGTACAGC ATACAGGCAG CGTTAGCTAT CCAGGACAGC 
CACAGAGAGC TCCATAGGTT ACTCCTGCAA GAGAATGAGA AGACCAGTCG TGGCCATAGT 
TCTCGGATAC CCCTCTTCCT GGAACCAGAA AAATACCGTA ATTCAGAAAA ACAAAGGGAG 
GAGCTGGCAA ACATCAACAA ACTCCAGCAG CAGTTCCAGC AAGAGCAGCA GCGCTGGCTG 
CGTGAATGCG AGCAGCGGCA GCAAGAGCTG GAGGTAAGGG CAGGCCTGCT GGAGCAGAGG 
GAGCAGGCCT GCAGCTGCCA GGAGGAGCTG CTGGAGAAGA GCCGCCAGGG GCTTGCACTG 
CAGTCGCAGG AGTTCCAGCA GAGCCTGGAG AGGCTGCGGG AAGGGCAGAA GTTGTTGGAG 
AAAAAAAGGG ACAGTATCAA AATGCAGAAG AAGCTCCTCT GGCACTGGAA GCATGGTCTC 
CAAAGCAGCC TGCTGCTGCC CACAGGGAGC CACGAGATAA TGGGACACGA TCAGTTGGAT 
AGCTTACATG GAGAAAATGC GTTCTATTTA AATGAAGCTG TAGTACGCGT GTCTCTAAGC 
GGCCTCAACA GATCAGATTC TTCTCTTGTT TATGAAGATG GTGTGTATGG GCTGGGCATT 
TCAGACTTGG ATATGGCAAG GACTTGTGAA AATCAGGTGA ACCTCAGGGT GGATACTCCT 
TGCCAACCAG CATTGACCAA TGAACTGTGG AAATCCACTG GCCCTTACCA GCAGATGTCT 
TTCTCCTGCA AAAGCAACAA GGATGCTTTT AATAATGATC TAAATGCATC ACAGACCCAG 
GGTCCCCAGG CAGGCAGTCC AAACCATGGA TCCATTCAGC CACCACAAGT GGAACCGCTG 
ATGCTGAACC ACCAGCCTGA GAGCCTGCAG GATGCGGAAA GTGGAGACGG AGGGGAGGAG 
AATATTGTTT ACCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	LOC427201
Entrez Gene ID	427201
Full Name	Rho guanine nucleotide exchange factor 28
General protein information	Preferred Names Rho guanine nucleotide exchange factor 28 Names rho guanine nucleotide exchange factor 28
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

Pan troglodytes (chimpanzee)	ARHGEF28	XP_001151239.2
Rattus norvegicus (Norway rat)	Arhgef28	NP_001102012.1
Gallus gallus (chicken)	LOC427201	XP_424789.4
Danio rerio (zebrafish)	arhgef28	XP_688428.4
Homo sapiens (human)	ARHGEF28	NP_001073948.2
Bos taurus (cattle)	RGNEF	NP_001095777.2
	ARHGEF28	XP_001101425.1
Canis lupus familiaris (dog)	ARHGEF28	XP_005617529.1
Mus musculus (house mouse)	Arhgef28	NP_036156.2
Xenopus tropicalis (tropical clawed frog)	arhgef28	XP_002934885.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC427201 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

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