ARHGEF28 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024981396.1	XP_024837164.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_024981394.1	XP_024837162.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_024981402.1	XP_024837170.1	rho guanine nucleotide exchange factor 28 isoform X8
XM_024981393.1	XP_024837161.1	rho guanine nucleotide exchange factor 28 isoform X1
XM_024981395.1	XP_024837163.1	rho guanine nucleotide exchange factor 28 isoform X3
XM_024981397.1	XP_024837165.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_024981398.1	XP_024837166.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_024981401.1	XP_024837169.1	rho guanine nucleotide exchange factor 28 isoform X7
XM_024981403.1	XP_024837171.1	rho guanine nucleotide exchange factor 28 isoform X9
XM_024981399.1	XP_024837167.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_024981400.1	XP_024837168.1	rho guanine nucleotide exchange factor 28 isoform X6
NM_001102307.1	NP_001095777.2	rho guanine nucleotide exchange factor 28

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa228079	XM_024981393.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228080	XM_024981394.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228081	XM_024981395.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228082	XM_024981396.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228083	XM_024981398.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228083	XM_024981397.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228083	XM_024981399.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228084	XM_024981400.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228085	XM_024981401.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228086	XM_024981402.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa228087	XM_024981403.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa39032	NM_001102307.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ARHGEF28 ( XM_024981393.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837161.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228079
Clone ID Related Accession (Same CDS sequence)	XM_024981393.1
Accession Version	XM_024981393.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG 
CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG 
GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC 
CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC 
CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT 
CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA 
GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC 
CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT 
CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG 
GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG 
TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC 
AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG 
CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA 
GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA 
TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT 
TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG 
GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC 
AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA 
TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA 
GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA 
ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC 
ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC 
TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG 
GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT 
GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG 
CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG 
CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG 
CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA 
ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT 
GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA 
AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT 
AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT 
AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC 
ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA 
GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT 
TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA 
AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT 
AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC 
AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG 
AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT 
GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT 
CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG 
ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT 
GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC 
AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA 
CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA 
GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG 
AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA 
GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT 
ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG 
ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA 
AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC 
TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG 
GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG 
GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG 
CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG 
CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG 
GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG 
AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG 
GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG 
TTTTCTCCAG GAGCCCTGGA GGTAATGGAA CTTAATCGAT CTGAGACTTT ACGTCATGAA 
AATTCATTCT TCATCAATGA AGCTTTAGTG CAAATGTCAC TCAACCCTTT GAACAAACCG 
CCGCCACCAG GTATCCATCA GGATGCGGCT TACCCCCCAG GCATACCTCA TTCTGATTTG 
GTAAGGACTA CTGAAAATCA AGTAGACCTC AAGACGGACG TTTCTCAACC CTCAGATATC 
AACCATGAAC TGTGGATAGC GGCTGGGGCC TGTCATCAGA TACCCCCTCT CCACCAAAGC 
AGCAAGGATT CTTGTAAAAA TGATGTGAAC ATCTCTCAGA CTGCATACCC ACCCCTCCAT 
GACTCAAATC CACACAGCCC TCGGCTGCAG ACGTTTATAG CAGAAGAAAA GCTAAATCTA 
CAGAGAGTGA CCAGACAAGA CGGGGAAACT GGAAATGGAG CAGAAGAAAA TATTGTTTAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837161.1
CDS	28..5433
Translation	MQPTLHFLPIFRCPFSCELCEEWIVSWSSLCNAEPIEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981393.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981393.1

ORF Insert Sequence:

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG 
CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG 
GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC 
CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC 
CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT 
CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA 
GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC 
CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT 
CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG 
GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG 
TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC 
AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG 
CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA 
GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA 
TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT 
TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG 
GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC 
AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA 
TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA 
GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA 
ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC 
ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC 
TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG 
GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT 
GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG 
CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG 
CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG 
CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA 
ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT 
GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA 
AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT 
AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT 
AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC 
ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA 
GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT 
TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA 
AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT 
AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC 
AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG 
AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT 
GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT 
CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG 
ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT 
GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC 
AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA 
CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA 
GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG 
AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA 
GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT 
ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG 
ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA 
AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC 
TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG 
GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG 
GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG 
CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG 
CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG 
GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG 
AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG 
GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG 
TTTTCTCCAG GAGCCCTGGA GGTAATGGAA CTTAATCGAT CTGAGACTTT ACGTCATGAA 
AATTCATTCT TCATCAATGA AGCTTTAGTG CAAATGTCAC TCAACCCTTT GAACAAACCG 
CCGCCACCAG GTATCCATCA GGATGCGGCT TACCCCCCAG GCATACCTCA TTCTGATTTG 
GTAAGGACTA CTGAAAATCA AGTAGACCTC AAGACGGACG TTTCTCAACC CTCAGATATC 
AACCATGAAC TGTGGATAGC GGCTGGGGCC TGTCATCAGA TACCCCCTCT CCACCAAAGC 
AGCAAGGATT CTTGTAAAAA TGATGTGAAC ATCTCTCAGA CTGCATACCC ACCCCTCCAT 
GACTCAAATC CACACAGCCC TCGGCTGCAG ACGTTTATAG CAGAAGAAAA GCTAAATCTA 
CAGAGAGTGA CCAGACAAGA CGGGGAAACT GGAAATGGAG CAGAAGAAAA TATTGTTTAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981394.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837162.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

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CloneID	OBa228080
Clone ID Related Accession (Same CDS sequence)	XM_024981394.1
Accession Version	XM_024981394.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGGTCA TTTTTGTTAG AAACACCCAA GATTGTCTTC TGTTTTCTCC TAGGTGGGAA 
GAAGATGCGG AAACCATGGA GCTGAACTGC AGCGAAGTAC CTCTGTATGG GCAGATGACG 
GTCTGCGCGA AGTTTGGCAA AAATGTTTAT CTTCCTAAAG ATGCTGAGTT TTACTTTATT 
TATAATGGAT CTCATCAGAG ACACATCGTG ATTGCAGAGC GCACAGAAGA TAATGTTCTC 
CAGTCCAGCA TTCCAGGCCA CGGGCTTCAG GAGACGGTCA CCGTGTCTGT GTGCCTGTGC 
TCGGAAGGTT ACTCCCCGGT GACCATGGGC TCTGACTCCG TGACCTACGT GGACAACATG 
GCCTGCAGGC TGGCCCGTCT GCTGGTCACC CAGGCCGATC GCCTCACCGC CAGCAGCCAC 
CAGACCTTGC TGACCCCGTT TGCCCTGACG GCGGGGGCAT TGCCTGCTCT GGACGAGGAG 
CTCGTGCTGG CCCTGACTCA TCTGGAGTTG CCTCTGGGGT GGACCGTCTT GGGGAATTCT 
TCACTGGAAG TTTCTCTGCA TAGGGAGTCT CTTCTGCACC TGGCTATGAG ATGGGGCCTT 
GCTAAGCTTT CCCAGCTCCT TCTGTGTCTC CCTGGGGGAG CCCAGGCCCT GACTTTACCC 
AACGAGGAGG GTGCCACGCC ATTAGACTTG GCTTTACATG GTGGACATTC CAAGCTGGTT 
GAAGACATCA CAAACTTCCA GGGCAGGCGT TCCCCAGGTT TCTCCTGCGT GCAGCTCAGC 
GAAGATGCCT CTCTGCGGTA TATTCACTCA TCAGAAACTC TGACCCTGAC GCTTAACCAC 
ACAGCCGAGC ATTTGCTGGA GGCGGATATT AAACTCTTCA GAAAATACTT TTGGGATAGA 
GCCTTTCTCA TCAAGGCTCT TGAAGAGCAA GAAGGCACGT CAGAGGAGAA ACCAGATATG 
CCCTCCAGCG CTGCAGAAAC TGAAGAAGGT ATGCATGCTC CTTCCCCACC TGGGGAACTC 
AGAAAGTGCT CAGCATTCTC TAGTCAATTG CGAGTTGAAG TGTGTGGAGA AGGAGAGCAG 
GAGCCGATGG ACTCGGAGGC GAGAGTAGGT TGCTCTGGGG CCGCAGGGCC CAGAGTTCCT 
TCAGCAGCAC TGACCAAAGA GATTAAGAAT TCGGGGTGCA GCAGATCACC ACCCGGGAAG 
GAGGGTGTAA AGCGTGTGAA AAGCCTGGTG GTTCCACTGA ATGAACATGA GGACCAGGAG 
AGGCTAGATT TGGATCGTTC CTTTGATGCC CTCAAAAAAT CCAAGCACCC CTCAACATTG 
CTTGCTGCTG GCCAGCTCTC TGACATGCTG AATGGTGGTG ATGACGTCTA CGCTAACTGC 
ATGGTGATAG ACCAGGTTGG TGATTTGGAC ATTAACTATA TTAATATAGA GGAAATCCCT 
GCAAATACCT GTCTTGAATC CATGGATTCT TCTCTGGGGC CTGAGAGCAG CAAACCCAGC 
CTTCCTACTG AAGCCAGTGC TGGCACATAC CCACCAAATA AGAGGCCAGA AGTGACGTCA 
CACACGGAAG CCCAATCATC TTTCATATCA TCTAGTTCGT ATGCTTCCAA TTTGAATCTT 
TCTTTTGGTC TTCGTGGATT TGAAAAGGAG CAAAGTCGTC TAAAGAAAAG AAGTTCCAGC 
CTTGATGCCT TGGATGCTGA CAGTGAAGGA GAGGGCCATT CGGAGCAGTC GCACAATTGC 
TACACTCCCG TGTGTCAGAG TTCCTCGAGA ACTGGCATTG CTAGCGGGGA TGAATTGGAC 
TCTTTTGAGA CCAACACTGA ACCAGATTTT AATATCTCCA GGACGGAATC ATTTTCTTTA 
TCAAGTAGCC TGCAGCTGAA GGAATCATTG TTTTCTGGAA TCCGTTCACG TTCGTATTCC 
TGCTCATCAC CCAAAATTTC TTTAGGAAAA ACTCGATTGA TGCGTGATTT TACAGTGTGT 
AATTCAATTG AAGAGCAAAG AGCTTACAGC TTGCCGGAGC CACCGAGAGA ACAAAGAATT 
CAGGAAGAAG AATGGGATAA ATATATCATA CCTGCCAAAT CAGAATCTGA AAAATACAAA 
GTAAGCCGAA CTTTTAGCTT CCTCATGAAT AGGATGACCA GCCCTCGGAA TAAATCAAAG 
ACAAAAGGCA AGGATGCCAA AGACAAAGAG AAACTGACTC GACATCAGTT TGTCCCTGGA 
ACTTTCTCTG GGGTTCTGCA GTGTTTGGTT TGCGATAAAA CGCTCCTGGG GAAAGAGTCA 
TTCCAGTGCT CCAACTGTAA TGGAAACGTA CATAAAGTCT GTAAAGATGC TGCCCCTCCG 
TGTACCAAGA AATTCCAAGA CAAATATAAT AAGAACAAAC CACAGACCAT CCTTGGAAAT 
TCTTCGTTTC GAGACGTCCC ATTGCCTGGG CTCTCTCTGC ACCCTTCCTC CTCCATGCCT 
ATCGGGTTGC CAACTGGGAG GAAGGAGATG GCAGGGCAAG TGCATCCCTT GTCCAGAAGT 
GTCCCAGGCA CCACCTTGGA AAGCTTCAGG AGGCCGGGGG CATCCTTGGA GTCCGAGAAT 
GATGGCAGCA CCTGGAGAAG CAGGTCTCAC TCTGACGAGC TGTTCCAGCC CATGGGCTCG 
TCCCCCTCCA CGGACTCCTT CTTAACGGAA GATGTCGTGG ATTCTTCTCT GTGGAGTGAC 
CTCAGCGGCG ATGCCCAGGA GTTTGAAGCG GAATCTTGGA GTCTGGTGGT GGACCCTTCA 
TTTTGTAGTC GGCAGGAGAA GGATGTCATC CAAAGACAGG ATGTCATTTT TGAGCTAATG 
CAGACAGAAC TGCATCACAT CCAGACCCTG TTCATCATGT CTGAGATCTT CAGGAAAGGG 
ATGAAAGAGG AGCTGCAGCT GGACCACAGC ACTGTGGACA AAATCTTCCC CTGTCTGGAC 
GAGTTACTTG AAATCCACAA GCATTTCTTC TGTAGTATGA AGGAGCGAAG GCAGGAATCC 
TGTGTTGGCA ATGACAGGAA TTTCATCATC GGCCGAATTG GAGATATTCT AGTACAACAG 
TTTTCAGAAG AAAATGCAAA TAAAATGAAG AAAATATATG GGGAATTCTG TAGCCATCAC 
AAAGAGGCTG TTAGCCTCTT TAAAGAACTC CAGCAGAATA AAAAGTTTCA GAATTTTATT 
AAGCTCCGAA ATAGTAACCT TCTGGCTCGA CGCCGAGGAA TTCCAGAATG CATTCTGCTG 
GTCACTCAGC GCATCACAAA ATACCCTGTC TTGGTGGAAA GGATATTGCA GTACACAAAG 
GAGAGCACTG AAGAACAGAG AGACTTATGC AAAGCTCTTT GCTTAATTAA AGACATGATT 
GCAGCTGTGG ATTTAAAAGT CAGCGAATAT GAGAAAAAAC AAAAATGGCT TGAGATCCTA 
AATAAGATCG AAAACAAAAC ATACACCAAG CTCAAAAATG GCCATGTGTT TAGGAAACAG 
GCACTGATGA GTAAAGAGAG GACCCTGTTA TATGATGGCC TTGTCTATTG GAAAACTGCT 
ACAGGTCGTT TCAAAGATAT CCTAGCTCTA CTTCTAACTG ATGTGCTGCT CTTTTTACAA 
GAAAAAGACC AGAAATACAT CTTTGCAGCT GTTGATCAGA AGCCATCAGT TATTTCCCTT 
CAGAAGCTCA TTGCTAGGGA AGTTGCGAAT GAGGAGAGAG GAATGTTTTT GATCAGCGCT 
TCATCTGCTG GTCCCGAGAT GTATGAAATC CATACCAACT CCAAGGAGGA AAGAAATACC 
TGGATGAGAC GGATCCAACA GGCAGTAGAA AGTTGCCCAG AAGAAGAAGG GGGAAGGACA 
AGTGAGTCTG ATGAAGAGAG GAGGAAAGCT GAAGCCAGAG TGGCGAAGAT TCAGCAATGC 
CAAGAAATAC TCAGTAACCA AGATCAACAA ATTTGCACAT ATTTGGAAGA GAAACTGCAT 
ATCTACGCGA AACTTGGAGA ACTAAGTGGA TTTCAGGATG TCCATCTAGA GCCCCACCTC 
CTCATTCAAC CAGACCCCGG AGAGCCTCCC CAGGCAGCCT CATTACTGGC CGCAGCACTG 
AAAGAGGCTG AGAGTCTGCA GGTGGCAGTG AAGGCTTCCC AGATGAGCGA TGCATGCCAG 
TCCCCCGAGG AAGGTTGTGG AGAACCTGCT TTGCCGGACG TGCTCCATTC TCAGGACGCA 
CCAGGATCGT CTACTGCTTC ACTAGTCACA GAAGGGACAG GAGGAAGAGG CTGTTGGGAT 
GTGGATGCCG GGACCCAGGG TGTGGGAACC GACCTGGCGG TTTCTGATGC GGGGGAGAAG 
GTGGAATATA GAAGTTTCCC AGGTTCTTTG CAGTCAGAGA TTATACAAGC GATACAGAAT 
TTAACTCGTC TCTTATATAG CCTTCAGGCC GCCTTGACCA TTCAGGATAG CCACATTGAG 
ATCCACAGGC TGGTTCTCCA ACAGCGGGAG AGCCTGGCCC CGGGCCCCGC GTTCCGAGGC 
AGCCCCTTTC AGGACCAGGA GAAGTCCCGC AGCCTGGAGA AGCAGCGGGA GGAGCTGGCC 
GATACCCACA AGCTGCAGCT CCAGTTCCAG CAAGACCAGC ACCGCTGGCT CCGCAGGTGC 
GACCAGCAGC AGCGCGAGCA GGAGGCCCGG GCCAGCCGGC TGCAGGAGCG CGAGAGGGAG 
TGCCGGGCGC AGGAGGAGCT GCTGCTCAGG AGCCGGGGCG AGCTGGACCA GCAGCTCCAA 
GACTACCAGC AGAGCCTGGA GCGCCTGCGC GAGGGCCAGC GCCTGGTGGA GAGGGAGCGG 
GAGAGGATGC GGGCCCAGCA GAGCCTGCTG CGCCACTGGA AGCACGGGCG GCAGAGCAGC 
CTTCCCGCCG CGTTTTCTCC AGGAGCCCTG GAGGTAATGG AACTTAATCG ATCTGAGACT 
TTACGTCATG AAAATTCATT CTTCATCAAT GAAGCTTTAG TGCAAATGTC ACTCAACCCT 
TTGAACAAAC CGCCGCCACC AGGTATCCAT CAGGATGCGG CTTACCCCCC AGGCATACCT 
CATTCTGATT TGGTAAGGAC TACTGAAAAT CAAGTAGACC TCAAGACGGA CGTTTCTCAA 
CCCTCAGATA TCAACCATGA ACTGTGGATA GCGGCTGGGG CCTGTCATCA GATACCCCCT 
CTCCACCAAA GCAGCAAGGA TTCTTGTAAA AATGATGTGA ACATCTCTCA GACTGCATAC 
CCACCCCTCC ATGACTCAAA TCCACACAGC CCTCGGCTGC AGACGTTTAT AGCAGAAGAA 
AAGCTAAATC TACAGAGAGT GACCAGACAA GACGGGGAAA CTGGAAATGG AGCAGAAGAA 
AATATTGTTT ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837162.1
CDS	102..5459
Translation	MRVIFVRNTQDCLLFSPRWEEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981394.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981394.1

ORF Insert Sequence:

ATGAGGGTCA TTTTTGTTAG AAACACCCAA GATTGTCTTC TGTTTTCTCC TAGGTGGGAA 
GAAGATGCGG AAACCATGGA GCTGAACTGC AGCGAAGTAC CTCTGTATGG GCAGATGACG 
GTCTGCGCGA AGTTTGGCAA AAATGTTTAT CTTCCTAAAG ATGCTGAGTT TTACTTTATT 
TATAATGGAT CTCATCAGAG ACACATCGTG ATTGCAGAGC GCACAGAAGA TAATGTTCTC 
CAGTCCAGCA TTCCAGGCCA CGGGCTTCAG GAGACGGTCA CCGTGTCTGT GTGCCTGTGC 
TCGGAAGGTT ACTCCCCGGT GACCATGGGC TCTGACTCCG TGACCTACGT GGACAACATG 
GCCTGCAGGC TGGCCCGTCT GCTGGTCACC CAGGCCGATC GCCTCACCGC CAGCAGCCAC 
CAGACCTTGC TGACCCCGTT TGCCCTGACG GCGGGGGCAT TGCCTGCTCT GGACGAGGAG 
CTCGTGCTGG CCCTGACTCA TCTGGAGTTG CCTCTGGGGT GGACCGTCTT GGGGAATTCT 
TCACTGGAAG TTTCTCTGCA TAGGGAGTCT CTTCTGCACC TGGCTATGAG ATGGGGCCTT 
GCTAAGCTTT CCCAGCTCCT TCTGTGTCTC CCTGGGGGAG CCCAGGCCCT GACTTTACCC 
AACGAGGAGG GTGCCACGCC ATTAGACTTG GCTTTACATG GTGGACATTC CAAGCTGGTT 
GAAGACATCA CAAACTTCCA GGGCAGGCGT TCCCCAGGTT TCTCCTGCGT GCAGCTCAGC 
GAAGATGCCT CTCTGCGGTA TATTCACTCA TCAGAAACTC TGACCCTGAC GCTTAACCAC 
ACAGCCGAGC ATTTGCTGGA GGCGGATATT AAACTCTTCA GAAAATACTT TTGGGATAGA 
GCCTTTCTCA TCAAGGCTCT TGAAGAGCAA GAAGGCACGT CAGAGGAGAA ACCAGATATG 
CCCTCCAGCG CTGCAGAAAC TGAAGAAGGT ATGCATGCTC CTTCCCCACC TGGGGAACTC 
AGAAAGTGCT CAGCATTCTC TAGTCAATTG CGAGTTGAAG TGTGTGGAGA AGGAGAGCAG 
GAGCCGATGG ACTCGGAGGC GAGAGTAGGT TGCTCTGGGG CCGCAGGGCC CAGAGTTCCT 
TCAGCAGCAC TGACCAAAGA GATTAAGAAT TCGGGGTGCA GCAGATCACC ACCCGGGAAG 
GAGGGTGTAA AGCGTGTGAA AAGCCTGGTG GTTCCACTGA ATGAACATGA GGACCAGGAG 
AGGCTAGATT TGGATCGTTC CTTTGATGCC CTCAAAAAAT CCAAGCACCC CTCAACATTG 
CTTGCTGCTG GCCAGCTCTC TGACATGCTG AATGGTGGTG ATGACGTCTA CGCTAACTGC 
ATGGTGATAG ACCAGGTTGG TGATTTGGAC ATTAACTATA TTAATATAGA GGAAATCCCT 
GCAAATACCT GTCTTGAATC CATGGATTCT TCTCTGGGGC CTGAGAGCAG CAAACCCAGC 
CTTCCTACTG AAGCCAGTGC TGGCACATAC CCACCAAATA AGAGGCCAGA AGTGACGTCA 
CACACGGAAG CCCAATCATC TTTCATATCA TCTAGTTCGT ATGCTTCCAA TTTGAATCTT 
TCTTTTGGTC TTCGTGGATT TGAAAAGGAG CAAAGTCGTC TAAAGAAAAG AAGTTCCAGC 
CTTGATGCCT TGGATGCTGA CAGTGAAGGA GAGGGCCATT CGGAGCAGTC GCACAATTGC 
TACACTCCCG TGTGTCAGAG TTCCTCGAGA ACTGGCATTG CTAGCGGGGA TGAATTGGAC 
TCTTTTGAGA CCAACACTGA ACCAGATTTT AATATCTCCA GGACGGAATC ATTTTCTTTA 
TCAAGTAGCC TGCAGCTGAA GGAATCATTG TTTTCTGGAA TCCGTTCACG TTCGTATTCC 
TGCTCATCAC CCAAAATTTC TTTAGGAAAA ACTCGATTGA TGCGTGATTT TACAGTGTGT 
AATTCAATTG AAGAGCAAAG AGCTTACAGC TTGCCGGAGC CACCGAGAGA ACAAAGAATT 
CAGGAAGAAG AATGGGATAA ATATATCATA CCTGCCAAAT CAGAATCTGA AAAATACAAA 
GTAAGCCGAA CTTTTAGCTT CCTCATGAAT AGGATGACCA GCCCTCGGAA TAAATCAAAG 
ACAAAAGGCA AGGATGCCAA AGACAAAGAG AAACTGACTC GACATCAGTT TGTCCCTGGA 
ACTTTCTCTG GGGTTCTGCA GTGTTTGGTT TGCGATAAAA CGCTCCTGGG GAAAGAGTCA 
TTCCAGTGCT CCAACTGTAA TGGAAACGTA CATAAAGTCT GTAAAGATGC TGCCCCTCCG 
TGTACCAAGA AATTCCAAGA CAAATATAAT AAGAACAAAC CACAGACCAT CCTTGGAAAT 
TCTTCGTTTC GAGACGTCCC ATTGCCTGGG CTCTCTCTGC ACCCTTCCTC CTCCATGCCT 
ATCGGGTTGC CAACTGGGAG GAAGGAGATG GCAGGGCAAG TGCATCCCTT GTCCAGAAGT 
GTCCCAGGCA CCACCTTGGA AAGCTTCAGG AGGCCGGGGG CATCCTTGGA GTCCGAGAAT 
GATGGCAGCA CCTGGAGAAG CAGGTCTCAC TCTGACGAGC TGTTCCAGCC CATGGGCTCG 
TCCCCCTCCA CGGACTCCTT CTTAACGGAA GATGTCGTGG ATTCTTCTCT GTGGAGTGAC 
CTCAGCGGCG ATGCCCAGGA GTTTGAAGCG GAATCTTGGA GTCTGGTGGT GGACCCTTCA 
TTTTGTAGTC GGCAGGAGAA GGATGTCATC CAAAGACAGG ATGTCATTTT TGAGCTAATG 
CAGACAGAAC TGCATCACAT CCAGACCCTG TTCATCATGT CTGAGATCTT CAGGAAAGGG 
ATGAAAGAGG AGCTGCAGCT GGACCACAGC ACTGTGGACA AAATCTTCCC CTGTCTGGAC 
GAGTTACTTG AAATCCACAA GCATTTCTTC TGTAGTATGA AGGAGCGAAG GCAGGAATCC 
TGTGTTGGCA ATGACAGGAA TTTCATCATC GGCCGAATTG GAGATATTCT AGTACAACAG 
TTTTCAGAAG AAAATGCAAA TAAAATGAAG AAAATATATG GGGAATTCTG TAGCCATCAC 
AAAGAGGCTG TTAGCCTCTT TAAAGAACTC CAGCAGAATA AAAAGTTTCA GAATTTTATT 
AAGCTCCGAA ATAGTAACCT TCTGGCTCGA CGCCGAGGAA TTCCAGAATG CATTCTGCTG 
GTCACTCAGC GCATCACAAA ATACCCTGTC TTGGTGGAAA GGATATTGCA GTACACAAAG 
GAGAGCACTG AAGAACAGAG AGACTTATGC AAAGCTCTTT GCTTAATTAA AGACATGATT 
GCAGCTGTGG ATTTAAAAGT CAGCGAATAT GAGAAAAAAC AAAAATGGCT TGAGATCCTA 
AATAAGATCG AAAACAAAAC ATACACCAAG CTCAAAAATG GCCATGTGTT TAGGAAACAG 
GCACTGATGA GTAAAGAGAG GACCCTGTTA TATGATGGCC TTGTCTATTG GAAAACTGCT 
ACAGGTCGTT TCAAAGATAT CCTAGCTCTA CTTCTAACTG ATGTGCTGCT CTTTTTACAA 
GAAAAAGACC AGAAATACAT CTTTGCAGCT GTTGATCAGA AGCCATCAGT TATTTCCCTT 
CAGAAGCTCA TTGCTAGGGA AGTTGCGAAT GAGGAGAGAG GAATGTTTTT GATCAGCGCT 
TCATCTGCTG GTCCCGAGAT GTATGAAATC CATACCAACT CCAAGGAGGA AAGAAATACC 
TGGATGAGAC GGATCCAACA GGCAGTAGAA AGTTGCCCAG AAGAAGAAGG GGGAAGGACA 
AGTGAGTCTG ATGAAGAGAG GAGGAAAGCT GAAGCCAGAG TGGCGAAGAT TCAGCAATGC 
CAAGAAATAC TCAGTAACCA AGATCAACAA ATTTGCACAT ATTTGGAAGA GAAACTGCAT 
ATCTACGCGA AACTTGGAGA ACTAAGTGGA TTTCAGGATG TCCATCTAGA GCCCCACCTC 
CTCATTCAAC CAGACCCCGG AGAGCCTCCC CAGGCAGCCT CATTACTGGC CGCAGCACTG 
AAAGAGGCTG AGAGTCTGCA GGTGGCAGTG AAGGCTTCCC AGATGAGCGA TGCATGCCAG 
TCCCCCGAGG AAGGTTGTGG AGAACCTGCT TTGCCGGACG TGCTCCATTC TCAGGACGCA 
CCAGGATCGT CTACTGCTTC ACTAGTCACA GAAGGGACAG GAGGAAGAGG CTGTTGGGAT 
GTGGATGCCG GGACCCAGGG TGTGGGAACC GACCTGGCGG TTTCTGATGC GGGGGAGAAG 
GTGGAATATA GAAGTTTCCC AGGTTCTTTG CAGTCAGAGA TTATACAAGC GATACAGAAT 
TTAACTCGTC TCTTATATAG CCTTCAGGCC GCCTTGACCA TTCAGGATAG CCACATTGAG 
ATCCACAGGC TGGTTCTCCA ACAGCGGGAG AGCCTGGCCC CGGGCCCCGC GTTCCGAGGC 
AGCCCCTTTC AGGACCAGGA GAAGTCCCGC AGCCTGGAGA AGCAGCGGGA GGAGCTGGCC 
GATACCCACA AGCTGCAGCT CCAGTTCCAG CAAGACCAGC ACCGCTGGCT CCGCAGGTGC 
GACCAGCAGC AGCGCGAGCA GGAGGCCCGG GCCAGCCGGC TGCAGGAGCG CGAGAGGGAG 
TGCCGGGCGC AGGAGGAGCT GCTGCTCAGG AGCCGGGGCG AGCTGGACCA GCAGCTCCAA 
GACTACCAGC AGAGCCTGGA GCGCCTGCGC GAGGGCCAGC GCCTGGTGGA GAGGGAGCGG 
GAGAGGATGC GGGCCCAGCA GAGCCTGCTG CGCCACTGGA AGCACGGGCG GCAGAGCAGC 
CTTCCCGCCG CGTTTTCTCC AGGAGCCCTG GAGGTAATGG AACTTAATCG ATCTGAGACT 
TTACGTCATG AAAATTCATT CTTCATCAAT GAAGCTTTAG TGCAAATGTC ACTCAACCCT 
TTGAACAAAC CGCCGCCACC AGGTATCCAT CAGGATGCGG CTTACCCCCC AGGCATACCT 
CATTCTGATT TGGTAAGGAC TACTGAAAAT CAAGTAGACC TCAAGACGGA CGTTTCTCAA 
CCCTCAGATA TCAACCATGA ACTGTGGATA GCGGCTGGGG CCTGTCATCA GATACCCCCT 
CTCCACCAAA GCAGCAAGGA TTCTTGTAAA AATGATGTGA ACATCTCTCA GACTGCATAC 
CCACCCCTCC ATGACTCAAA TCCACACAGC CCTCGGCTGC AGACGTTTAT AGCAGAAGAA 
AAGCTAAATC TACAGAGAGT GACCAGACAA GACGGGGAAA CTGGAAATGG AGCAGAAGAA 
AATATTGTTT ACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981395.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837163.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa228081
Clone ID Related Accession (Same CDS sequence)	XM_024981395.1
Accession Version	XM_024981395.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5301bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG 
CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG 
GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC 
CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC 
CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT 
CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA 
GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC 
CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT 
CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG 
GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG 
TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC 
AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG 
CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA 
GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA 
TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT 
TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG 
GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC 
AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG 
CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA 
AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG 
AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC 
TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT 
GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT 
TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA 
ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA 
GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG 
GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA 
TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA 
CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT 
CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT 
ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG 
CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA 
AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG 
ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC 
CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA 
CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT 
GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA 
CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC 
CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC 
GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT 
ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG 
ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA 
TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG 
CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC 
CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA 
CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC 
CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC 
GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG 
GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG 
AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG 
AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT 
GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA 
GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG 
GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG 
TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG 
GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC 
CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG 
CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC 
AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG 
ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC 
CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA 
CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT 
CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC 
CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA 
TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA 
GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA 
GAAAATATTG TTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837163.1
CDS	118..5418
Translation	MQPTLHFLPIFRCPFSCELCEEWIVSWSSLCNAEPIEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981395.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981395.1

ORF Insert Sequence:

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG 
CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG 
GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC 
CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC 
CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT 
CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA 
GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC 
CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT 
CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG 
GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG 
TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC 
AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG 
CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA 
GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA 
TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT 
TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG 
GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC 
AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG 
CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA 
AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG 
AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC 
TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT 
GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT 
TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA 
ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA 
GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG 
GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA 
TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA 
CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT 
CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT 
ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG 
CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA 
AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG 
ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC 
CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA 
CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT 
GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA 
CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC 
CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC 
GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT 
ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG 
ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA 
TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG 
CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC 
CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA 
CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC 
CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC 
GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG 
GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG 
AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG 
AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT 
GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA 
GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG 
GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG 
TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG 
GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC 
CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG 
CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC 
AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG 
ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC 
CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA 
CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT 
CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC 
CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA 
TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA 
GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA 
GAAAATATTG TTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981396.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837164.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

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CloneID	OBa228082
Clone ID Related Accession (Same CDS sequence)	XM_024981396.1
Accession Version	XM_024981396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGATC GTTCCTTTGA TGCCCTCAAA AAATCCAAGC ACCCCTCAAC ATTGCTTGCT 
GCTGGCCAGC TCTCTGACAT GCTGAATGGT GGTGATGACG TCTACGCTAA CTGCATGGTG 
ATAGACCAGG TTGGTGATTT GGACATTAAC TATATTAATA TAGAGGAAAT CCCTGCAAAT 
ACCTGTCTTG AATCCATGGA TTCTTCTCTG GGGCCTGAGA GCAGCAAACC CAGCCTTCCT 
ACTGAAGCCA GTGCTGGCAC ATACCCACCA AATAAGAGGC CAGAAGTGAC GTCACACACG 
GAAGCCCAAT CATCTTTCAT ATCATCTAGT TCGTATGCTT CCAATTTGAA TCTTTCTTTT 
GGTCTTCGTG GATTTGAAAA GGAGCAAAGT CGTCTAAAGA AAAGAAGTTC CAGCCTTGAT 
GCCTTGGATG CTGACAGTGA AGGAGAGGGC CATTCGGAGC AGTCGCACAA TTGCTACACT 
CCCGTGTGTC AGAGTTCCTC GAGAACTGGC ATTGCTAGCG GGGATGAATT GGACTCTTTT 
GAGACCAACA CTGAACCAGA TTTTAATATC TCCAGGACGG AATCATTTTC TTTATCAAGT 
AGCCTGCAGC TGAAGGAATC ATTGTTTTCT GGAATCCGTT CACGTTCGTA TTCCTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGA TTGATGCGTG ATTTTACAGT GTGTAATTCA 
ATTGAAGAGC AAAGAGCTTA CAGCTTGCCG GAGCCACCGA GAGAACAAAG AATTCAGGAA 
GAAGAATGGG ATAAATATAT CATACCTGCC AAATCAGAAT CTGAAAAATA CAAAGTAAGC 
CGAACTTTTA GCTTCCTCAT GAATAGGATG ACCAGCCCTC GGAATAAATC AAAGACAAAA 
GGCAAGGATG CCAAAGACAA AGAGAAACTG ACTCGACATC AGTTTGTCCC TGGAACTTTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGCGAT AAAACGCTCC TGGGGAAAGA GTCATTCCAG 
TGCTCCAACT GTAATGGAAA CGTACATAAA GTCTGTAAAG ATGCTGCCCC TCCGTGTACC 
AAGAAATTCC AAGACAAATA TAATAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCG 
TTTCGAGACG TCCCATTGCC TGGGCTCTCT CTGCACCCTT CCTCCTCCAT GCCTATCGGG 
TTGCCAACTG GGAGGAAGGA GATGGCAGGG CAAGTGCATC CCTTGTCCAG AAGTGTCCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGCCG GGGGCATCCT TGGAGTCCGA GAATGATGGC 
AGCACCTGGA GAAGCAGGTC TCACTCTGAC GAGCTGTTCC AGCCCATGGG CTCGTCCCCC 
TCCACGGACT CCTTCTTAAC GGAAGATGTC GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
GGCGATGCCC AGGAGTTTGA AGCGGAATCT TGGAGTCTGG TGGTGGACCC TTCATTTTGT 
AGTCGGCAGG AGAAGGATGT CATCCAAAGA CAGGATGTCA TTTTTGAGCT AATGCAGACA 
GAACTGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGGATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACTGTG GACAAAATCT TCCCCTGTCT GGACGAGTTA 
CTTGAAATCC ACAAGCATTT CTTCTGTAGT ATGAAGGAGC GAAGGCAGGA ATCCTGTGTT 
GGCAATGACA GGAATTTCAT CATCGGCCGA ATTGGAGATA TTCTAGTACA ACAGTTTTCA 
GAAGAAAATG CAAATAAAAT GAAGAAAATA TATGGGGAAT TCTGTAGCCA TCACAAAGAG 
GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ACCTTCTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GCTGGTCACT 
CAGCGCATCA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAGAGC 
ACTGAAGAAC AGAGAGACTT ATGCAAAGCT CTTTGCTTAA TTAAAGACAT GATTGCAGCT 
GTGGATTTAA AAGTCAGCGA ATATGAGAAA AAACAAAAAT GGCTTGAGAT CCTAAATAAG 
ATCGAAAACA AAACATACAC CAAGCTCAAA AATGGCCATG TGTTTAGGAA ACAGGCACTG 
ATGAGTAAAG AGAGGACCCT GTTATATGAT GGCCTTGTCT ATTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCTGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAGAAG 
CTCATTGCTA GGGAAGTTGC GAATGAGGAG AGAGGAATGT TTTTGATCAG CGCTTCATCT 
GCTGGTCCCG AGATGTATGA AATCCATACC AACTCCAAGG AGGAAAGAAA TACCTGGATG 
AGACGGATCC AACAGGCAGT AGAAAGTTGC CCAGAAGAAG AAGGGGGAAG GACAAGTGAG 
TCTGATGAAG AGAGGAGGAA AGCTGAAGCC AGAGTGGCGA AGATTCAGCA ATGCCAAGAA 
ATACTCAGTA ACCAAGATCA ACAAATTTGC ACATATTTGG AAGAGAAACT GCATATCTAC 
GCGAAACTTG GAGAACTAAG TGGATTTCAG GATGTCCATC TAGAGCCCCA CCTCCTCATT 
CAACCAGACC CCGGAGAGCC TCCCCAGGCA GCCTCATTAC TGGCCGCAGC ACTGAAAGAG 
GCTGAGAGTC TGCAGGTGGC AGTGAAGGCT TCCCAGATGA GCGATGCATG CCAGTCCCCC 
GAGGAAGGTT GTGGAGAACC TGCTTTGCCG GACGTGCTCC ATTCTCAGGA CGCACCAGGA 
TCGTCTACTG CTTCACTAGT CACAGAAGGG ACAGGAGGAA GAGGCTGTTG GGATGTGGAT 
GCCGGGACCC AGGGTGTGGG AACCGACCTG GCGGTTTCTG ATGCGGGGGA GAAGGTGGAA 
TATAGAAGTT TCCCAGGTTC TTTGCAGTCA GAGATTATAC AAGCGATACA GAATTTAACT 
CGTCTCTTAT ATAGCCTTCA GGCCGCCTTG ACCATTCAGG ATAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAACAGCG GGAGAGCCTG GCCCCGGGCC CCGCGTTCCG AGGCAGCCCC 
TTTCAGGACC AGGAGAAGTC CCGCAGCCTG GAGAAGCAGC GGGAGGAGCT GGCCGATACC 
CACAAGCTGC AGCTCCAGTT CCAGCAAGAC CAGCACCGCT GGCTCCGCAG GTGCGACCAG 
CAGCAGCGCG AGCAGGAGGC CCGGGCCAGC CGGCTGCAGG AGCGCGAGAG GGAGTGCCGG 
GCGCAGGAGG AGCTGCTGCT CAGGAGCCGG GGCGAGCTGG ACCAGCAGCT CCAAGACTAC 
CAGCAGAGCC TGGAGCGCCT GCGCGAGGGC CAGCGCCTGG TGGAGAGGGA GCGGGAGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGCGCCAC TGGAAGCACG GGCGGCAGAG CAGCCTTCCC 
GCCGCGTTTT CTCCAGGAGC CCTGGAGGTA ATGGAACTTA ATCGATCTGA GACTTTACGT 
CATGAAAATT CATTCTTCAT CAATGAAGCT TTAGTGCAAA TGTCACTCAA CCCTTTGAAC 
AAACCGCCGC CACCAGGTAT CCATCAGGAT GCGGCTTACC CCCCAGGCAT ACCTCATTCT 
GATTTGGTAA GGACTACTGA AAATCAAGTA GACCTCAAGA CGGACGTTTC TCAACCCTCA 
GATATCAACC ATGAACTGTG GATAGCGGCT GGGGCCTGTC ATCAGATACC CCCTCTCCAC 
CAAAGCAGCA AGGATTCTTG TAAAAATGAT GTGAACATCT CTCAGACTGC ATACCCACCC 
CTCCATGACT CAAATCCACA CAGCCCTCGG CTGCAGACGT TTATAGCAGA AGAAAAGCTA 
AATCTACAGA GAGTGACCAG ACAAGACGGG GAAACTGGAA ATGGAGCAGA AGAAAATATT 
GTTTACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837164.1
CDS	19..5310
Translation	MQPTLHFLPIFRCPFSCELCEEWIVSWSSLCNAEPIEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981396.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981396.1

ORF Insert Sequence:

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGATC GTTCCTTTGA TGCCCTCAAA AAATCCAAGC ACCCCTCAAC ATTGCTTGCT 
GCTGGCCAGC TCTCTGACAT GCTGAATGGT GGTGATGACG TCTACGCTAA CTGCATGGTG 
ATAGACCAGG TTGGTGATTT GGACATTAAC TATATTAATA TAGAGGAAAT CCCTGCAAAT 
ACCTGTCTTG AATCCATGGA TTCTTCTCTG GGGCCTGAGA GCAGCAAACC CAGCCTTCCT 
ACTGAAGCCA GTGCTGGCAC ATACCCACCA AATAAGAGGC CAGAAGTGAC GTCACACACG 
GAAGCCCAAT CATCTTTCAT ATCATCTAGT TCGTATGCTT CCAATTTGAA TCTTTCTTTT 
GGTCTTCGTG GATTTGAAAA GGAGCAAAGT CGTCTAAAGA AAAGAAGTTC CAGCCTTGAT 
GCCTTGGATG CTGACAGTGA AGGAGAGGGC CATTCGGAGC AGTCGCACAA TTGCTACACT 
CCCGTGTGTC AGAGTTCCTC GAGAACTGGC ATTGCTAGCG GGGATGAATT GGACTCTTTT 
GAGACCAACA CTGAACCAGA TTTTAATATC TCCAGGACGG AATCATTTTC TTTATCAAGT 
AGCCTGCAGC TGAAGGAATC ATTGTTTTCT GGAATCCGTT CACGTTCGTA TTCCTGCTCA 
TCACCCAAAA TTTCTTTAGG AAAAACTCGA TTGATGCGTG ATTTTACAGT GTGTAATTCA 
ATTGAAGAGC AAAGAGCTTA CAGCTTGCCG GAGCCACCGA GAGAACAAAG AATTCAGGAA 
GAAGAATGGG ATAAATATAT CATACCTGCC AAATCAGAAT CTGAAAAATA CAAAGTAAGC 
CGAACTTTTA GCTTCCTCAT GAATAGGATG ACCAGCCCTC GGAATAAATC AAAGACAAAA 
GGCAAGGATG CCAAAGACAA AGAGAAACTG ACTCGACATC AGTTTGTCCC TGGAACTTTC 
TCTGGGGTTC TGCAGTGTTT GGTTTGCGAT AAAACGCTCC TGGGGAAAGA GTCATTCCAG 
TGCTCCAACT GTAATGGAAA CGTACATAAA GTCTGTAAAG ATGCTGCCCC TCCGTGTACC 
AAGAAATTCC AAGACAAATA TAATAAGAAC AAACCACAGA CCATCCTTGG AAATTCTTCG 
TTTCGAGACG TCCCATTGCC TGGGCTCTCT CTGCACCCTT CCTCCTCCAT GCCTATCGGG 
TTGCCAACTG GGAGGAAGGA GATGGCAGGG CAAGTGCATC CCTTGTCCAG AAGTGTCCCA 
GGCACCACCT TGGAAAGCTT CAGGAGGCCG GGGGCATCCT TGGAGTCCGA GAATGATGGC 
AGCACCTGGA GAAGCAGGTC TCACTCTGAC GAGCTGTTCC AGCCCATGGG CTCGTCCCCC 
TCCACGGACT CCTTCTTAAC GGAAGATGTC GTGGATTCTT CTCTGTGGAG TGACCTCAGC 
GGCGATGCCC AGGAGTTTGA AGCGGAATCT TGGAGTCTGG TGGTGGACCC TTCATTTTGT 
AGTCGGCAGG AGAAGGATGT CATCCAAAGA CAGGATGTCA TTTTTGAGCT AATGCAGACA 
GAACTGCATC ACATCCAGAC CCTGTTCATC ATGTCTGAGA TCTTCAGGAA AGGGATGAAA 
GAGGAGCTGC AGCTGGACCA CAGCACTGTG GACAAAATCT TCCCCTGTCT GGACGAGTTA 
CTTGAAATCC ACAAGCATTT CTTCTGTAGT ATGAAGGAGC GAAGGCAGGA ATCCTGTGTT 
GGCAATGACA GGAATTTCAT CATCGGCCGA ATTGGAGATA TTCTAGTACA ACAGTTTTCA 
GAAGAAAATG CAAATAAAAT GAAGAAAATA TATGGGGAAT TCTGTAGCCA TCACAAAGAG 
GCTGTTAGCC TCTTTAAAGA ACTCCAGCAG AATAAAAAGT TTCAGAATTT TATTAAGCTC 
CGAAATAGTA ACCTTCTGGC TCGACGCCGA GGAATTCCAG AATGCATTCT GCTGGTCACT 
CAGCGCATCA CAAAATACCC TGTCTTGGTG GAAAGGATAT TGCAGTACAC AAAGGAGAGC 
ACTGAAGAAC AGAGAGACTT ATGCAAAGCT CTTTGCTTAA TTAAAGACAT GATTGCAGCT 
GTGGATTTAA AAGTCAGCGA ATATGAGAAA AAACAAAAAT GGCTTGAGAT CCTAAATAAG 
ATCGAAAACA AAACATACAC CAAGCTCAAA AATGGCCATG TGTTTAGGAA ACAGGCACTG 
ATGAGTAAAG AGAGGACCCT GTTATATGAT GGCCTTGTCT ATTGGAAAAC TGCTACAGGT 
CGTTTCAAAG ATATCCTAGC TCTACTTCTA ACTGATGTGC TGCTCTTTTT ACAAGAAAAA 
GACCAGAAAT ACATCTTTGC AGCTGTTGAT CAGAAGCCAT CAGTTATTTC CCTTCAGAAG 
CTCATTGCTA GGGAAGTTGC GAATGAGGAG AGAGGAATGT TTTTGATCAG CGCTTCATCT 
GCTGGTCCCG AGATGTATGA AATCCATACC AACTCCAAGG AGGAAAGAAA TACCTGGATG 
AGACGGATCC AACAGGCAGT AGAAAGTTGC CCAGAAGAAG AAGGGGGAAG GACAAGTGAG 
TCTGATGAAG AGAGGAGGAA AGCTGAAGCC AGAGTGGCGA AGATTCAGCA ATGCCAAGAA 
ATACTCAGTA ACCAAGATCA ACAAATTTGC ACATATTTGG AAGAGAAACT GCATATCTAC 
GCGAAACTTG GAGAACTAAG TGGATTTCAG GATGTCCATC TAGAGCCCCA CCTCCTCATT 
CAACCAGACC CCGGAGAGCC TCCCCAGGCA GCCTCATTAC TGGCCGCAGC ACTGAAAGAG 
GCTGAGAGTC TGCAGGTGGC AGTGAAGGCT TCCCAGATGA GCGATGCATG CCAGTCCCCC 
GAGGAAGGTT GTGGAGAACC TGCTTTGCCG GACGTGCTCC ATTCTCAGGA CGCACCAGGA 
TCGTCTACTG CTTCACTAGT CACAGAAGGG ACAGGAGGAA GAGGCTGTTG GGATGTGGAT 
GCCGGGACCC AGGGTGTGGG AACCGACCTG GCGGTTTCTG ATGCGGGGGA GAAGGTGGAA 
TATAGAAGTT TCCCAGGTTC TTTGCAGTCA GAGATTATAC AAGCGATACA GAATTTAACT 
CGTCTCTTAT ATAGCCTTCA GGCCGCCTTG ACCATTCAGG ATAGCCACAT TGAGATCCAC 
AGGCTGGTTC TCCAACAGCG GGAGAGCCTG GCCCCGGGCC CCGCGTTCCG AGGCAGCCCC 
TTTCAGGACC AGGAGAAGTC CCGCAGCCTG GAGAAGCAGC GGGAGGAGCT GGCCGATACC 
CACAAGCTGC AGCTCCAGTT CCAGCAAGAC CAGCACCGCT GGCTCCGCAG GTGCGACCAG 
CAGCAGCGCG AGCAGGAGGC CCGGGCCAGC CGGCTGCAGG AGCGCGAGAG GGAGTGCCGG 
GCGCAGGAGG AGCTGCTGCT CAGGAGCCGG GGCGAGCTGG ACCAGCAGCT CCAAGACTAC 
CAGCAGAGCC TGGAGCGCCT GCGCGAGGGC CAGCGCCTGG TGGAGAGGGA GCGGGAGAGG 
ATGCGGGCCC AGCAGAGCCT GCTGCGCCAC TGGAAGCACG GGCGGCAGAG CAGCCTTCCC 
GCCGCGTTTT CTCCAGGAGC CCTGGAGGTA ATGGAACTTA ATCGATCTGA GACTTTACGT 
CATGAAAATT CATTCTTCAT CAATGAAGCT TTAGTGCAAA TGTCACTCAA CCCTTTGAAC 
AAACCGCCGC CACCAGGTAT CCATCAGGAT GCGGCTTACC CCCCAGGCAT ACCTCATTCT 
GATTTGGTAA GGACTACTGA AAATCAAGTA GACCTCAAGA CGGACGTTTC TCAACCCTCA 
GATATCAACC ATGAACTGTG GATAGCGGCT GGGGCCTGTC ATCAGATACC CCCTCTCCAC 
CAAAGCAGCA AGGATTCTTG TAAAAATGAT GTGAACATCT CTCAGACTGC ATACCCACCC 
CTCCATGACT CAAATCCACA CAGCCCTCGG CTGCAGACGT TTATAGCAGA AGAAAAGCTA 
AATCTACAGA GAGTGACCAG ACAAGACGGG GAAACTGGAA ATGGAGCAGA AGAAAATATT 
GTTTACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981397.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837165.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

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CloneID	OBa228083
Clone ID Related Accession (Same CDS sequence)	XM_024981397.1 , XM_024981398.1 , XM_024981399.1
Accession Version	XM_024981397.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT 
CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA 
GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC 
CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC 
CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG 
ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT 
CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG 
CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC 
ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC 
TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG 
CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG 
CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA 
GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA 
TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG 
GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC 
AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT 
GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT 
CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG 
CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG 
GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT 
GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC 
AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA 
TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT 
GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT 
GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT 
CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC 
ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG 
CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA 
ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG 
CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG 
GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT 
AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT 
GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT 
CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC 
TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC 
CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC 
GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT 
GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC 
TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG 
AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC 
TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC 
CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG 
GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT 
CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG 
CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC 
CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC 
AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT 
GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC 
CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT 
AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC 
ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA 
CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA 
AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC 
AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA 
GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA 
GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT 
AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC 
GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC 
CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA 
GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT 
AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT 
GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC 
CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT 
CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT 
TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT 
GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC 
CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT 
TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA 
TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT 
CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC 
CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG 
CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC 
GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG 
GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC 
CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC 
CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT 
TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT 
TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG 
CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA 
AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC 
CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC 
TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG 
AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837165.1
CDS	138..5420
Translation	MELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981397.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981397.1

ORF Insert Sequence:

ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT 
CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA 
GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC 
CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC 
CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG 
ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT 
CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG 
CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC 
ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC 
TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG 
CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG 
CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA 
GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA 
TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG 
GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC 
AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT 
GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT 
CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG 
CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG 
GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT 
GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC 
AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA 
TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT 
GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT 
GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT 
CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC 
ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG 
CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA 
ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG 
CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG 
GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT 
AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT 
GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT 
CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC 
TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC 
CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC 
GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT 
GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC 
TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG 
AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC 
TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC 
CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG 
GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT 
CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG 
CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC 
CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC 
AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT 
GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC 
CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT 
AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC 
ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA 
CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA 
AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC 
AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA 
GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA 
GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT 
AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC 
GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC 
CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA 
GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT 
AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT 
GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC 
CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT 
CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT 
TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT 
GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC 
CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT 
TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA 
TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT 
CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC 
CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG 
CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC 
GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG 
GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC 
CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC 
CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT 
TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT 
TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG 
CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA 
AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC 
CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC 
TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG 
AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981398.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837166.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.

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CloneID	OBa228083
Clone ID Related Accession (Same CDS sequence)	XM_024981397.1 , XM_024981398.1 , XM_024981399.1
Accession Version	XM_024981398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT 
CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA 
GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC 
CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC 
CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG 
ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT 
CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG 
CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC 
ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC 
TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG 
CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG 
CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA 
GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA 
TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG 
GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC 
AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT 
GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT 
CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG 
CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG 
GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT 
GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC 
AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA 
TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT 
GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT 
GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT 
CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC 
ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG 
CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA 
ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG 
CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG 
GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT 
AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT 
GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT 
CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC 
TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC 
CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC 
GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT 
GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC 
TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG 
AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC 
TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC 
CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG 
GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT 
CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG 
CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC 
CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC 
AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT 
GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC 
CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT 
AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC 
ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA 
CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA 
AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC 
AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA 
GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA 
GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT 
AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC 
GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC 
CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA 
GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT 
AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT 
GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC 
CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT 
CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT 
TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT 
GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC 
CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT 
TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA 
TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT 
CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC 
CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG 
CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC 
GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG 
GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC 
CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC 
CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT 
TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT 
TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG 
CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA 
AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC 
CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC 
TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG 
AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837166.1
CDS	203..5485
Translation	MELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981398.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981398.1

ORF Insert Sequence:

ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT 
CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA 
GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC 
CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC 
CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG 
ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT 
CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG 
CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC 
ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC 
TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG 
CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG 
CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA 
GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA 
TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG 
GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC 
AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT 
GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT 
CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG 
CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG 
GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT 
GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC 
AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA 
TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT 
GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT 
GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT 
CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC 
ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG 
CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA 
ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG 
CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG 
GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT 
AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT 
GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT 
CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC 
TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC 
CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC 
GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT 
GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC 
TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG 
AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC 
TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC 
CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG 
GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT 
CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG 
CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC 
CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC 
AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT 
GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC 
CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT 
AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC 
ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA 
CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA 
AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC 
AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA 
GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA 
GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT 
AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC 
GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC 
CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA 
GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT 
AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT 
GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC 
CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT 
CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT 
TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT 
GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC 
CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT 
TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA 
TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT 
CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC 
CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG 
CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC 
GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG 
GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC 
CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC 
CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT 
TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT 
TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG 
CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA 
AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC 
CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC 
TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG 
AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981399.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837167.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA.

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CloneID	OBa228083
Clone ID Related Accession (Same CDS sequence)	XM_024981397.1 , XM_024981398.1 , XM_024981399.1
Accession Version	XM_024981399.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT 
CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA 
GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC 
CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC 
CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG 
ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT 
CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG 
CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC 
ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC 
TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG 
CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG 
CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA 
GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA 
TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG 
GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC 
AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT 
GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT 
CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG 
CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG 
GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT 
GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC 
AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA 
TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT 
GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT 
GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT 
CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC 
ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG 
CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA 
ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG 
CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG 
GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT 
AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT 
GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT 
CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC 
TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC 
CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC 
GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT 
GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC 
TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG 
AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC 
TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC 
CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG 
GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT 
CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG 
CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC 
CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC 
AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT 
GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC 
CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT 
AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC 
ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA 
CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA 
AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC 
AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA 
GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA 
GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT 
AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC 
GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC 
CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA 
GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT 
AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT 
GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC 
CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT 
CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT 
TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT 
GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC 
CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT 
TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA 
TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT 
CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC 
CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG 
CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC 
GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG 
GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC 
CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC 
CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT 
TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT 
TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG 
CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA 
AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC 
CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC 
TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG 
AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837167.1
CDS	232..5514
Translation	MELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981399.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981399.1

ORF Insert Sequence:

ATGGAGCTGA ACTGCAGCGA AGTACCTCTG TATGGGCAGA TGACGGTCTG CGCGAAGTTT 
GGCAAAAATG TTTATCTTCC TAAAGATGCT GAGTTTTACT TTATTTATAA TGGATCTCAT 
CAGAGACACA TCGTGATTGC AGAGCGCACA GAAGATAATG TTCTCCAGTC CAGCATTCCA 
GGCCACGGGC TTCAGGAGAC GGTCACCGTG TCTGTGTGCC TGTGCTCGGA AGGTTACTCC 
CCGGTGACCA TGGGCTCTGA CTCCGTGACC TACGTGGACA ACATGGCCTG CAGGCTGGCC 
CGTCTGCTGG TCACCCAGGC CGATCGCCTC ACCGCCAGCA GCCACCAGAC CTTGCTGACC 
CCGTTTGCCC TGACGGCGGG GGCATTGCCT GCTCTGGACG AGGAGCTCGT GCTGGCCCTG 
ACTCATCTGG AGTTGCCTCT GGGGTGGACC GTCTTGGGGA ATTCTTCACT GGAAGTTTCT 
CTGCATAGGG AGTCTCTTCT GCACCTGGCT ATGAGATGGG GCCTTGCTAA GCTTTCCCAG 
CTCCTTCTGT GTCTCCCTGG GGGAGCCCAG GCCCTGACTT TACCCAACGA GGAGGGTGCC 
ACGCCATTAG ACTTGGCTTT ACATGGTGGA CATTCCAAGC TGGTTGAAGA CATCACAAAC 
TTCCAGGGCA GGCGTTCCCC AGGTTTCTCC TGCGTGCAGC TCAGCGAAGA TGCCTCTCTG 
CGGTATATTC ACTCATCAGA AACTCTGACC CTGACGCTTA ACCACACAGC CGAGCATTTG 
CTGGAGGCGG ATATTAAACT CTTCAGAAAA TACTTTTGGG ATAGAGCCTT TCTCATCAAG 
GCTCTTGAAG AGCAAGAAGG CACGTCAGAG GAGAAACCAG ATATGCCCTC CAGCGCTGCA 
GAAACTGAAG AAGGTATGCA TGCTCCTTCC CCACCTGGGG AACTCAGAAA GTGCTCAGCA 
TTCTCTAGTC AATTGCGAGT TGAAGTGTGT GGAGAAGGAG AGCAGGAGCC GATGGACTCG 
GAGGCGAGAG TAGGTTGCTC TGGGGCCGCA GGGCCCAGAG TTCCTTCAGC AGCACTGACC 
AAAGAGATTA AGAATTCGGG GTGCAGCAGA TCACCACCCG GGAAGGAGGG TGTAAAGCGT 
GTGAAAAGCC TGGTGGTTCC ACTGAATGAA CATGAGGACC AGGAGAGGCT AGATTTGGAT 
CGTTCCTTTG ATGCCCTCAA AAAATCCAAG CACCCCTCAA CATTGCTTGC TGCTGGCCAG 
CTCTCTGACA TGCTGAATGG TGGTGATGAC GTCTACGCTA ACTGCATGGT GATAGACCAG 
GTTGGTGATT TGGACATTAA CTATATTAAT ATAGAGGAAA TCCCTGCAAA TACCTGTCTT 
GAATCCATGG ATTCTTCTCT GGGGCCTGAG AGCAGCAAAC CCAGCCTTCC TACTGAAGCC 
AGTGCTGGCA CATACCCACC AAATAAGAGG CCAGAAGTGA CGTCACACAC GGAAGCCCAA 
TCATCTTTCA TATCATCTAG TTCGTATGCT TCCAATTTGA ATCTTTCTTT TGGTCTTCGT 
GGATTTGAAA AGGAGCAAAG TCGTCTAAAG AAAAGAAGTT CCAGCCTTGA TGCCTTGGAT 
GCTGACAGTG AAGGAGAGGG CCATTCGGAG CAGTCGCACA ATTGCTACAC TCCCGTGTGT 
CAGAGTTCCT CGAGAACTGG CATTGCTAGC GGGGATGAAT TGGACTCTTT TGAGACCAAC 
ACTGAACCAG ATTTTAATAT CTCCAGGACG GAATCATTTT CTTTATCAAG TAGCCTGCAG 
CTGAAGGAAT CATTGTTTTC TGGAATCCGT TCACGTTCGT ATTCCTGCTC ATCACCCAAA 
ATTTCTTTAG GAAAAACTCG ATTGATGCGT GATTTTACAG TGTGTAATTC AATTGAAGAG 
CAAAGAGCTT ACAGCTTGCC GGAGCCACCG AGAGAACAAA GAATTCAGGA AGAAGAATGG 
GATAAATATA TCATACCTGC CAAATCAGAA TCTGAAAAAT ACAAAGTAAG CCGAACTTTT 
AGCTTCCTCA TGAATAGGAT GACCAGCCCT CGGAATAAAT CAAAGACAAA AGGCAAGGAT 
GCCAAAGACA AAGAGAAACT GACTCGACAT CAGTTTGTCC CTGGAACTTT CTCTGGGGTT 
CTGCAGTGTT TGGTTTGCGA TAAAACGCTC CTGGGGAAAG AGTCATTCCA GTGCTCCAAC 
TGTAATGGAA ACGTACATAA AGTCTGTAAA GATGCTGCCC CTCCGTGTAC CAAGAAATTC 
CAAGACAAAT ATAATAAGAA CAAACCACAG ACCATCCTTG GAAATTCTTC GTTTCGAGAC 
GTCCCATTGC CTGGGCTCTC TCTGCACCCT TCCTCCTCCA TGCCTATCGG GTTGCCAACT 
GGGAGGAAGG AGATGGCAGG GCAAGTGCAT CCCTTGTCCA GAAGTGTCCC AGGCACCACC 
TTGGAAAGCT TCAGGAGGCC GGGGGCATCC TTGGAGTCCG AGAATGATGG CAGCACCTGG 
AGAAGCAGGT CTCACTCTGA CGAGCTGTTC CAGCCCATGG GCTCGTCCCC CTCCACGGAC 
TCCTTCTTAA CGGAAGATGT CGTGGATTCT TCTCTGTGGA GTGACCTCAG CGGCGATGCC 
CAGGAGTTTG AAGCGGAATC TTGGAGTCTG GTGGTGGACC CTTCATTTTG TAGTCGGCAG 
GAGAAGGATG TCATCCAAAG ACAGGATGTC ATTTTTGAGC TAATGCAGAC AGAACTGCAT 
CACATCCAGA CCCTGTTCAT CATGTCTGAG ATCTTCAGGA AAGGGATGAA AGAGGAGCTG 
CAGCTGGACC ACAGCACTGT GGACAAAATC TTCCCCTGTC TGGACGAGTT ACTTGAAATC 
CACAAGCATT TCTTCTGTAG TATGAAGGAG CGAAGGCAGG AATCCTGTGT TGGCAATGAC 
AGGAATTTCA TCATCGGCCG AATTGGAGAT ATTCTAGTAC AACAGTTTTC AGAAGAAAAT 
GCAAATAAAA TGAAGAAAAT ATATGGGGAA TTCTGTAGCC ATCACAAAGA GGCTGTTAGC 
CTCTTTAAAG AACTCCAGCA GAATAAAAAG TTTCAGAATT TTATTAAGCT CCGAAATAGT 
AACCTTCTGG CTCGACGCCG AGGAATTCCA GAATGCATTC TGCTGGTCAC TCAGCGCATC 
ACAAAATACC CTGTCTTGGT GGAAAGGATA TTGCAGTACA CAAAGGAGAG CACTGAAGAA 
CAGAGAGACT TATGCAAAGC TCTTTGCTTA ATTAAAGACA TGATTGCAGC TGTGGATTTA 
AAAGTCAGCG AATATGAGAA AAAACAAAAA TGGCTTGAGA TCCTAAATAA GATCGAAAAC 
AAAACATACA CCAAGCTCAA AAATGGCCAT GTGTTTAGGA AACAGGCACT GATGAGTAAA 
GAGAGGACCC TGTTATATGA TGGCCTTGTC TATTGGAAAA CTGCTACAGG TCGTTTCAAA 
GATATCCTAG CTCTACTTCT AACTGATGTG CTGCTCTTTT TACAAGAAAA AGACCAGAAA 
TACATCTTTG CAGCTGTTGA TCAGAAGCCA TCAGTTATTT CCCTTCAGAA GCTCATTGCT 
AGGGAAGTTG CGAATGAGGA GAGAGGAATG TTTTTGATCA GCGCTTCATC TGCTGGTCCC 
GAGATGTATG AAATCCATAC CAACTCCAAG GAGGAAAGAA ATACCTGGAT GAGACGGATC 
CAACAGGCAG TAGAAAGTTG CCCAGAAGAA GAAGGGGGAA GGACAAGTGA GTCTGATGAA 
GAGAGGAGGA AAGCTGAAGC CAGAGTGGCG AAGATTCAGC AATGCCAAGA AATACTCAGT 
AACCAAGATC AACAAATTTG CACATATTTG GAAGAGAAAC TGCATATCTA CGCGAAACTT 
GGAGAACTAA GTGGATTTCA GGATGTCCAT CTAGAGCCCC ACCTCCTCAT TCAACCAGAC 
CCCGGAGAGC CTCCCCAGGC AGCCTCATTA CTGGCCGCAG CACTGAAAGA GGCTGAGAGT 
CTGCAGGTGG CAGTGAAGGC TTCCCAGATG AGCGATGCAT GCCAGTCCCC CGAGGAAGGT 
TGTGGAGAAC CTGCTTTGCC GGACGTGCTC CATTCTCAGG ACGCACCAGG ATCGTCTACT 
GCTTCACTAG TCACAGAAGG GACAGGAGGA AGAGGCTGTT GGGATGTGGA TGCCGGGACC 
CAGGGTGTGG GAACCGACCT GGCGGTTTCT GATGCGGGGG AGAAGGTGGA ATATAGAAGT 
TTCCCAGGTT CTTTGCAGTC AGAGATTATA CAAGCGATAC AGAATTTAAC TCGTCTCTTA 
TATAGCCTTC AGGCCGCCTT GACCATTCAG GATAGCCACA TTGAGATCCA CAGGCTGGTT 
CTCCAACAGC GGGAGAGCCT GGCCCCGGGC CCCGCGTTCC GAGGCAGCCC CTTTCAGGAC 
CAGGAGAAGT CCCGCAGCCT GGAGAAGCAG CGGGAGGAGC TGGCCGATAC CCACAAGCTG 
CAGCTCCAGT TCCAGCAAGA CCAGCACCGC TGGCTCCGCA GGTGCGACCA GCAGCAGCGC 
GAGCAGGAGG CCCGGGCCAG CCGGCTGCAG GAGCGCGAGA GGGAGTGCCG GGCGCAGGAG 
GAGCTGCTGC TCAGGAGCCG GGGCGAGCTG GACCAGCAGC TCCAAGACTA CCAGCAGAGC 
CTGGAGCGCC TGCGCGAGGG CCAGCGCCTG GTGGAGAGGG AGCGGGAGAG GATGCGGGCC 
CAGCAGAGCC TGCTGCGCCA CTGGAAGCAC GGGCGGCAGA GCAGCCTTCC CGCCGCGTTT 
TCTCCAGGAG CCCTGGAGGT AATGGAACTT AATCGATCTG AGACTTTACG TCATGAAAAT 
TCATTCTTCA TCAATGAAGC TTTAGTGCAA ATGTCACTCA ACCCTTTGAA CAAACCGCCG 
CCACCAGGTA TCCATCAGGA TGCGGCTTAC CCCCCAGGCA TACCTCATTC TGATTTGGTA 
AGGACTACTG AAAATCAAGT AGACCTCAAG ACGGACGTTT CTCAACCCTC AGATATCAAC 
CATGAACTGT GGATAGCGGC TGGGGCCTGT CATCAGATAC CCCCTCTCCA CCAAAGCAGC 
AAGGATTCTT GTAAAAATGA TGTGAACATC TCTCAGACTG CATACCCACC CCTCCATGAC 
TCAAATCCAC ACAGCCCTCG GCTGCAGACG TTTATAGCAG AAGAAAAGCT AAATCTACAG 
AGAGTGACCA GACAAGACGG GGAAACTGGA AATGGAGCAG AAGAAAATAT TGTTTACCTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981400.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837168.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA.

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CloneID	OBa228084
Clone ID Related Accession (Same CDS sequence)	XM_024981400.1
Accession Version	XM_024981400.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGTCT GCGCGAAGTT TGGCAAAAAT GTTTATCTTC CTAAAGATGC TGAGTTTTAC 
TTTATTTATA ATGGATCTCA TCAGAGACAC ATCGTGATTG CAGAGCGCAC AGAAGATAAT 
GTTCTCCAGT CCAGCATTCC AGGCCACGGG CTTCAGGAGA CGGTCACCGT GTCTGTGTGC 
CTGTGCTCGG AAGGTTACTC CCCGGTGACC ATGGGCTCTG ACTCCGTGAC CTACGTGGAC 
AACATGGCCT GCAGGCTGGC CCGTCTGCTG GTCACCCAGG CCGATCGCCT CACCGCCAGC 
AGCCACCAGA CCTTGCTGAC CCCGTTTGCC CTGACGGCGG GGGCATTGCC TGCTCTGGAC 
GAGGAGCTCG TGCTGGCCCT GACTCATCTG GAGTTGCCTC TGGGGTGGAC CGTCTTGGGG 
AATTCTTCAC TGGAAGTTTC TCTGCATAGG GAGTCTCTTC TGCACCTGGC TATGAGATGG 
GGCCTTGCTA AGCTTTCCCA GCTCCTTCTG TGTCTCCCTG GGGGAGCCCA GGCCCTGACT 
TTACCCAACG AGGAGGGTGC CACGCCATTA GACTTGGCTT TACATGGTGG ACATTCCAAG 
CTGGTTGAAG ACATCACAAA CTTCCAGGGC AGGCGTTCCC CAGGTTTCTC CTGCGTGCAG 
CTCAGCGAAG ATGCCTCTCT GCGGTATATT CACTCATCAG AAACTCTGAC CCTGACGCTT 
AACCACACAG CCGAGCATTT GCTGGAGGCG GATATTAAAC TCTTCAGAAA ATACTTTTGG 
GATAGAGCCT TTCTCATCAA GGCTCTTGAA GAGCAAGAAG GCACGTCAGA GGAGAAACCA 
GATATGCCCT CCAGCGCTGC AGAAACTGAA GAAGGTATGC ATGCTCCTTC CCCACCTGGG 
GAACTCAGAA AGTGCTCAGC ATTCTCTAGT CAATTGCGAG TTGAAGTGTG TGGAGAAGGA 
GAGCAGGAGC CGATGGACTC GGAGGCGAGA GTAGGTTGCT CTGGGGCCGC AGGGCCCAGA 
GTTCCTTCAG CAGCACTGAC CAAAGAGATT AAGAATTCGG GGTGCAGCAG ATCACCACCC 
GGGAAGGAGG GTGTAAAGCG TGTGAAAAGC CTGGTGGTTC CACTGAATGA ACATGAGGAC 
CAGGAGAGGC TAGATTTGGA TCGTTCCTTT GATGCCCTCA AAAAATCCAA GCACCCCTCA 
ACATTGCTTG CTGCTGGCCA GCTCTCTGAC ATGCTGAATG GTGGTGATGA CGTCTACGCT 
AACTGCATGG TGATAGACCA GGTTGGTGAT TTGGACATTA ACTATATTAA TATAGAGGAA 
ATCCCTGCAA ATACCTGTCT TGAATCCATG GATTCTTCTC TGGGGCCTGA GAGCAGCAAA 
CCCAGCCTTC CTACTGAAGC CAGTGCTGGC ACATACCCAC CAAATAAGAG GCCAGAAGTG 
ACGTCACACA CGGAAGCCCA ATCATCTTTC ATATCATCTA GTTCGTATGC TTCCAATTTG 
AATCTTTCTT TTGGTCTTCG TGGATTTGAA AAGGAGCAAA GTCGTCTAAA GAAAAGAAGT 
TCCAGCCTTG ATGCCTTGGA TGCTGACAGT GAAGGAGAGG GCCATTCGGA GCAGTCGCAC 
AATTGCTACA CTCCCGTGTG TCAGAGTTCC TCGAGAACTG GCATTGCTAG CGGGGATGAA 
TTGGACTCTT TTGAGACCAA CACTGAACCA GATTTTAATA TCTCCAGGAC GGAATCATTT 
TCTTTATCAA GTAGCCTGCA GCTGAAGGAA TCATTGTTTT CTGGAATCCG TTCACGTTCG 
TATTCCTGCT CATCACCCAA AATTTCTTTA GGAAAAACTC GATTGATGCG TGATTTTACA 
GTGTGTAATT CAATTGAAGA GCAAAGAGCT TACAGCTTGC CGGAGCCACC GAGAGAACAA 
AGAATTCAGG AAGAAGAATG GGATAAATAT ATCATACCTG CCAAATCAGA ATCTGAAAAA 
TACAAAGTAA GCCGAACTTT TAGCTTCCTC ATGAATAGGA TGACCAGCCC TCGGAATAAA 
TCAAAGACAA AAGGCAAGGA TGCCAAAGAC AAAGAGAAAC TGACTCGACA TCAGTTTGTC 
CCTGGAACTT TCTCTGGGGT TCTGCAGTGT TTGGTTTGCG ATAAAACGCT CCTGGGGAAA 
GAGTCATTCC AGTGCTCCAA CTGTAATGGA AACGTACATA AAGTCTGTAA AGATGCTGCC 
CCTCCGTGTA CCAAGAAATT CCAAGACAAA TATAATAAGA ACAAACCACA GACCATCCTT 
GGAAATTCTT CGTTTCGAGA CGTCCCATTG CCTGGGCTCT CTCTGCACCC TTCCTCCTCC 
ATGCCTATCG GGTTGCCAAC TGGGAGGAAG GAGATGGCAG GGCAAGTGCA TCCCTTGTCC 
AGAAGTGTCC CAGGCACCAC CTTGGAAAGC TTCAGGAGGC CGGGGGCATC CTTGGAGTCC 
GAGAATGATG GCAGCACCTG GAGAAGCAGG TCTCACTCTG ACGAGCTGTT CCAGCCCATG 
GGCTCGTCCC CCTCCACGGA CTCCTTCTTA ACGGAAGATG TCGTGGATTC TTCTCTGTGG 
AGTGACCTCA GCGGCGATGC CCAGGAGTTT GAAGCGGAAT CTTGGAGTCT GGTGGTGGAC 
CCTTCATTTT GTAGTCGGCA GGAGAAGGAT GTCATCCAAA GACAGGATGT CATTTTTGAG 
CTAATGCAGA CAGAACTGCA TCACATCCAG ACCCTGTTCA TCATGTCTGA GATCTTCAGG 
AAAGGGATGA AAGAGGAGCT GCAGCTGGAC CACAGCACTG TGGACAAAAT CTTCCCCTGT 
CTGGACGAGT TACTTGAAAT CCACAAGCAT TTCTTCTGTA GTATGAAGGA GCGAAGGCAG 
GAATCCTGTG TTGGCAATGA CAGGAATTTC ATCATCGGCC GAATTGGAGA TATTCTAGTA 
CAACAGTTTT CAGAAGAAAA TGCAAATAAA ATGAAGAAAA TATATGGGGA ATTCTGTAGC 
CATCACAAAG AGGCTGTTAG CCTCTTTAAA GAACTCCAGC AGAATAAAAA GTTTCAGAAT 
TTTATTAAGC TCCGAAATAG TAACCTTCTG GCTCGACGCC GAGGAATTCC AGAATGCATT 
CTGCTGGTCA CTCAGCGCAT CACAAAATAC CCTGTCTTGG TGGAAAGGAT ATTGCAGTAC 
ACAAAGGAGA GCACTGAAGA ACAGAGAGAC TTATGCAAAG CTCTTTGCTT AATTAAAGAC 
ATGATTGCAG CTGTGGATTT AAAAGTCAGC GAATATGAGA AAAAACAAAA ATGGCTTGAG 
ATCCTAAATA AGATCGAAAA CAAAACATAC ACCAAGCTCA AAAATGGCCA TGTGTTTAGG 
AAACAGGCAC TGATGAGTAA AGAGAGGACC CTGTTATATG ATGGCCTTGT CTATTGGAAA 
ACTGCTACAG GTCGTTTCAA AGATATCCTA GCTCTACTTC TAACTGATGT GCTGCTCTTT 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ATCAGAAGCC ATCAGTTATT 
TCCCTTCAGA AGCTCATTGC TAGGGAAGTT GCGAATGAGG AGAGAGGAAT GTTTTTGATC 
AGCGCTTCAT CTGCTGGTCC CGAGATGTAT GAAATCCATA CCAACTCCAA GGAGGAAAGA 
AATACCTGGA TGAGACGGAT CCAACAGGCA GTAGAAAGTT GCCCAGAAGA AGAAGGGGGA 
AGGACAAGTG AGTCTGATGA AGAGAGGAGG AAAGCTGAAG CCAGAGTGGC GAAGATTCAG 
CAATGCCAAG AAATACTCAG TAACCAAGAT CAACAAATTT GCACATATTT GGAAGAGAAA 
CTGCATATCT ACGCGAAACT TGGAGAACTA AGTGGATTTC AGGATGTCCA TCTAGAGCCC 
CACCTCCTCA TTCAACCAGA CCCCGGAGAG CCTCCCCAGG CAGCCTCATT ACTGGCCGCA 
GCACTGAAAG AGGCTGAGAG TCTGCAGGTG GCAGTGAAGG CTTCCCAGAT GAGCGATGCA 
TGCCAGTCCC CCGAGGAAGG TTGTGGAGAA CCTGCTTTGC CGGACGTGCT CCATTCTCAG 
GACGCACCAG GATCGTCTAC TGCTTCACTA GTCACAGAAG GGACAGGAGG AAGAGGCTGT 
TGGGATGTGG ATGCCGGGAC CCAGGGTGTG GGAACCGACC TGGCGGTTTC TGATGCGGGG 
GAGAAGGTGG AATATAGAAG TTTCCCAGGT TCTTTGCAGT CAGAGATTAT ACAAGCGATA 
CAGAATTTAA CTCGTCTCTT ATATAGCCTT CAGGCCGCCT TGACCATTCA GGATAGCCAC 
ATTGAGATCC ACAGGCTGGT TCTCCAACAG CGGGAGAGCC TGGCCCCGGG CCCCGCGTTC 
CGAGGCAGCC CCTTTCAGGA CCAGGAGAAG TCCCGCAGCC TGGAGAAGCA GCGGGAGGAG 
CTGGCCGATA CCCACAAGCT GCAGCTCCAG TTCCAGCAAG ACCAGCACCG CTGGCTCCGC 
AGGTGCGACC AGCAGCAGCG CGAGCAGGAG GCCCGGGCCA GCCGGCTGCA GGAGCGCGAG 
AGGGAGTGCC GGGCGCAGGA GGAGCTGCTG CTCAGGAGCC GGGGCGAGCT GGACCAGCAG 
CTCCAAGACT ACCAGCAGAG CCTGGAGCGC CTGCGCGAGG GCCAGCGCCT GGTGGAGAGG 
GAGCGGGAGA GGATGCGGGC CCAGCAGAGC CTGCTGCGCC ACTGGAAGCA CGGGCGGCAG 
AGCAGCCTTC CCGCCGCGTT TTCTCCAGGA GCCCTGGAGG TAATGGAACT TAATCGATCT 
GAGACTTTAC GTCATGAAAA TTCATTCTTC ATCAATGAAG CTTTAGTGCA AATGTCACTC 
AACCCTTTGA ACAAACCGCC GCCACCAGGT ATCCATCAGG ATGCGGCTTA CCCCCCAGGC 
ATACCTCATT CTGATTTGGT AAGGACTACT GAAAATCAAG TAGACCTCAA GACGGACGTT 
TCTCAACCCT CAGATATCAA CCATGAACTG TGGATAGCGG CTGGGGCCTG TCATCAGATA 
CCCCCTCTCC ACCAAAGCAG CAAGGATTCT TGTAAAAATG ATGTGAACAT CTCTCAGACT 
GCATACCCAC CCCTCCATGA CTCAAATCCA CACAGCCCTC GGCTGCAGAC GTTTATAGCA 
GAAGAAAAGC TAAATCTACA GAGAGTGACC AGACAAGACG GGGAAACTGG AAATGGAGCA 
GAAGAAAATA TTGTTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837168.1
CDS	1573..6816
Translation	MTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981400.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981400.1

ORF Insert Sequence:

ATGACGGTCT GCGCGAAGTT TGGCAAAAAT GTTTATCTTC CTAAAGATGC TGAGTTTTAC 
TTTATTTATA ATGGATCTCA TCAGAGACAC ATCGTGATTG CAGAGCGCAC AGAAGATAAT 
GTTCTCCAGT CCAGCATTCC AGGCCACGGG CTTCAGGAGA CGGTCACCGT GTCTGTGTGC 
CTGTGCTCGG AAGGTTACTC CCCGGTGACC ATGGGCTCTG ACTCCGTGAC CTACGTGGAC 
AACATGGCCT GCAGGCTGGC CCGTCTGCTG GTCACCCAGG CCGATCGCCT CACCGCCAGC 
AGCCACCAGA CCTTGCTGAC CCCGTTTGCC CTGACGGCGG GGGCATTGCC TGCTCTGGAC 
GAGGAGCTCG TGCTGGCCCT GACTCATCTG GAGTTGCCTC TGGGGTGGAC CGTCTTGGGG 
AATTCTTCAC TGGAAGTTTC TCTGCATAGG GAGTCTCTTC TGCACCTGGC TATGAGATGG 
GGCCTTGCTA AGCTTTCCCA GCTCCTTCTG TGTCTCCCTG GGGGAGCCCA GGCCCTGACT 
TTACCCAACG AGGAGGGTGC CACGCCATTA GACTTGGCTT TACATGGTGG ACATTCCAAG 
CTGGTTGAAG ACATCACAAA CTTCCAGGGC AGGCGTTCCC CAGGTTTCTC CTGCGTGCAG 
CTCAGCGAAG ATGCCTCTCT GCGGTATATT CACTCATCAG AAACTCTGAC CCTGACGCTT 
AACCACACAG CCGAGCATTT GCTGGAGGCG GATATTAAAC TCTTCAGAAA ATACTTTTGG 
GATAGAGCCT TTCTCATCAA GGCTCTTGAA GAGCAAGAAG GCACGTCAGA GGAGAAACCA 
GATATGCCCT CCAGCGCTGC AGAAACTGAA GAAGGTATGC ATGCTCCTTC CCCACCTGGG 
GAACTCAGAA AGTGCTCAGC ATTCTCTAGT CAATTGCGAG TTGAAGTGTG TGGAGAAGGA 
GAGCAGGAGC CGATGGACTC GGAGGCGAGA GTAGGTTGCT CTGGGGCCGC AGGGCCCAGA 
GTTCCTTCAG CAGCACTGAC CAAAGAGATT AAGAATTCGG GGTGCAGCAG ATCACCACCC 
GGGAAGGAGG GTGTAAAGCG TGTGAAAAGC CTGGTGGTTC CACTGAATGA ACATGAGGAC 
CAGGAGAGGC TAGATTTGGA TCGTTCCTTT GATGCCCTCA AAAAATCCAA GCACCCCTCA 
ACATTGCTTG CTGCTGGCCA GCTCTCTGAC ATGCTGAATG GTGGTGATGA CGTCTACGCT 
AACTGCATGG TGATAGACCA GGTTGGTGAT TTGGACATTA ACTATATTAA TATAGAGGAA 
ATCCCTGCAA ATACCTGTCT TGAATCCATG GATTCTTCTC TGGGGCCTGA GAGCAGCAAA 
CCCAGCCTTC CTACTGAAGC CAGTGCTGGC ACATACCCAC CAAATAAGAG GCCAGAAGTG 
ACGTCACACA CGGAAGCCCA ATCATCTTTC ATATCATCTA GTTCGTATGC TTCCAATTTG 
AATCTTTCTT TTGGTCTTCG TGGATTTGAA AAGGAGCAAA GTCGTCTAAA GAAAAGAAGT 
TCCAGCCTTG ATGCCTTGGA TGCTGACAGT GAAGGAGAGG GCCATTCGGA GCAGTCGCAC 
AATTGCTACA CTCCCGTGTG TCAGAGTTCC TCGAGAACTG GCATTGCTAG CGGGGATGAA 
TTGGACTCTT TTGAGACCAA CACTGAACCA GATTTTAATA TCTCCAGGAC GGAATCATTT 
TCTTTATCAA GTAGCCTGCA GCTGAAGGAA TCATTGTTTT CTGGAATCCG TTCACGTTCG 
TATTCCTGCT CATCACCCAA AATTTCTTTA GGAAAAACTC GATTGATGCG TGATTTTACA 
GTGTGTAATT CAATTGAAGA GCAAAGAGCT TACAGCTTGC CGGAGCCACC GAGAGAACAA 
AGAATTCAGG AAGAAGAATG GGATAAATAT ATCATACCTG CCAAATCAGA ATCTGAAAAA 
TACAAAGTAA GCCGAACTTT TAGCTTCCTC ATGAATAGGA TGACCAGCCC TCGGAATAAA 
TCAAAGACAA AAGGCAAGGA TGCCAAAGAC AAAGAGAAAC TGACTCGACA TCAGTTTGTC 
CCTGGAACTT TCTCTGGGGT TCTGCAGTGT TTGGTTTGCG ATAAAACGCT CCTGGGGAAA 
GAGTCATTCC AGTGCTCCAA CTGTAATGGA AACGTACATA AAGTCTGTAA AGATGCTGCC 
CCTCCGTGTA CCAAGAAATT CCAAGACAAA TATAATAAGA ACAAACCACA GACCATCCTT 
GGAAATTCTT CGTTTCGAGA CGTCCCATTG CCTGGGCTCT CTCTGCACCC TTCCTCCTCC 
ATGCCTATCG GGTTGCCAAC TGGGAGGAAG GAGATGGCAG GGCAAGTGCA TCCCTTGTCC 
AGAAGTGTCC CAGGCACCAC CTTGGAAAGC TTCAGGAGGC CGGGGGCATC CTTGGAGTCC 
GAGAATGATG GCAGCACCTG GAGAAGCAGG TCTCACTCTG ACGAGCTGTT CCAGCCCATG 
GGCTCGTCCC CCTCCACGGA CTCCTTCTTA ACGGAAGATG TCGTGGATTC TTCTCTGTGG 
AGTGACCTCA GCGGCGATGC CCAGGAGTTT GAAGCGGAAT CTTGGAGTCT GGTGGTGGAC 
CCTTCATTTT GTAGTCGGCA GGAGAAGGAT GTCATCCAAA GACAGGATGT CATTTTTGAG 
CTAATGCAGA CAGAACTGCA TCACATCCAG ACCCTGTTCA TCATGTCTGA GATCTTCAGG 
AAAGGGATGA AAGAGGAGCT GCAGCTGGAC CACAGCACTG TGGACAAAAT CTTCCCCTGT 
CTGGACGAGT TACTTGAAAT CCACAAGCAT TTCTTCTGTA GTATGAAGGA GCGAAGGCAG 
GAATCCTGTG TTGGCAATGA CAGGAATTTC ATCATCGGCC GAATTGGAGA TATTCTAGTA 
CAACAGTTTT CAGAAGAAAA TGCAAATAAA ATGAAGAAAA TATATGGGGA ATTCTGTAGC 
CATCACAAAG AGGCTGTTAG CCTCTTTAAA GAACTCCAGC AGAATAAAAA GTTTCAGAAT 
TTTATTAAGC TCCGAAATAG TAACCTTCTG GCTCGACGCC GAGGAATTCC AGAATGCATT 
CTGCTGGTCA CTCAGCGCAT CACAAAATAC CCTGTCTTGG TGGAAAGGAT ATTGCAGTAC 
ACAAAGGAGA GCACTGAAGA ACAGAGAGAC TTATGCAAAG CTCTTTGCTT AATTAAAGAC 
ATGATTGCAG CTGTGGATTT AAAAGTCAGC GAATATGAGA AAAAACAAAA ATGGCTTGAG 
ATCCTAAATA AGATCGAAAA CAAAACATAC ACCAAGCTCA AAAATGGCCA TGTGTTTAGG 
AAACAGGCAC TGATGAGTAA AGAGAGGACC CTGTTATATG ATGGCCTTGT CTATTGGAAA 
ACTGCTACAG GTCGTTTCAA AGATATCCTA GCTCTACTTC TAACTGATGT GCTGCTCTTT 
TTACAAGAAA AAGACCAGAA ATACATCTTT GCAGCTGTTG ATCAGAAGCC ATCAGTTATT 
TCCCTTCAGA AGCTCATTGC TAGGGAAGTT GCGAATGAGG AGAGAGGAAT GTTTTTGATC 
AGCGCTTCAT CTGCTGGTCC CGAGATGTAT GAAATCCATA CCAACTCCAA GGAGGAAAGA 
AATACCTGGA TGAGACGGAT CCAACAGGCA GTAGAAAGTT GCCCAGAAGA AGAAGGGGGA 
AGGACAAGTG AGTCTGATGA AGAGAGGAGG AAAGCTGAAG CCAGAGTGGC GAAGATTCAG 
CAATGCCAAG AAATACTCAG TAACCAAGAT CAACAAATTT GCACATATTT GGAAGAGAAA 
CTGCATATCT ACGCGAAACT TGGAGAACTA AGTGGATTTC AGGATGTCCA TCTAGAGCCC 
CACCTCCTCA TTCAACCAGA CCCCGGAGAG CCTCCCCAGG CAGCCTCATT ACTGGCCGCA 
GCACTGAAAG AGGCTGAGAG TCTGCAGGTG GCAGTGAAGG CTTCCCAGAT GAGCGATGCA 
TGCCAGTCCC CCGAGGAAGG TTGTGGAGAA CCTGCTTTGC CGGACGTGCT CCATTCTCAG 
GACGCACCAG GATCGTCTAC TGCTTCACTA GTCACAGAAG GGACAGGAGG AAGAGGCTGT 
TGGGATGTGG ATGCCGGGAC CCAGGGTGTG GGAACCGACC TGGCGGTTTC TGATGCGGGG 
GAGAAGGTGG AATATAGAAG TTTCCCAGGT TCTTTGCAGT CAGAGATTAT ACAAGCGATA 
CAGAATTTAA CTCGTCTCTT ATATAGCCTT CAGGCCGCCT TGACCATTCA GGATAGCCAC 
ATTGAGATCC ACAGGCTGGT TCTCCAACAG CGGGAGAGCC TGGCCCCGGG CCCCGCGTTC 
CGAGGCAGCC CCTTTCAGGA CCAGGAGAAG TCCCGCAGCC TGGAGAAGCA GCGGGAGGAG 
CTGGCCGATA CCCACAAGCT GCAGCTCCAG TTCCAGCAAG ACCAGCACCG CTGGCTCCGC 
AGGTGCGACC AGCAGCAGCG CGAGCAGGAG GCCCGGGCCA GCCGGCTGCA GGAGCGCGAG 
AGGGAGTGCC GGGCGCAGGA GGAGCTGCTG CTCAGGAGCC GGGGCGAGCT GGACCAGCAG 
CTCCAAGACT ACCAGCAGAG CCTGGAGCGC CTGCGCGAGG GCCAGCGCCT GGTGGAGAGG 
GAGCGGGAGA GGATGCGGGC CCAGCAGAGC CTGCTGCGCC ACTGGAAGCA CGGGCGGCAG 
AGCAGCCTTC CCGCCGCGTT TTCTCCAGGA GCCCTGGAGG TAATGGAACT TAATCGATCT 
GAGACTTTAC GTCATGAAAA TTCATTCTTC ATCAATGAAG CTTTAGTGCA AATGTCACTC 
AACCCTTTGA ACAAACCGCC GCCACCAGGT ATCCATCAGG ATGCGGCTTA CCCCCCAGGC 
ATACCTCATT CTGATTTGGT AAGGACTACT GAAAATCAAG TAGACCTCAA GACGGACGTT 
TCTCAACCCT CAGATATCAA CCATGAACTG TGGATAGCGG CTGGGGCCTG TCATCAGATA 
CCCCCTCTCC ACCAAAGCAG CAAGGATTCT TGTAAAAATG ATGTGAACAT CTCTCAGACT 
GCATACCCAC CCCTCCATGA CTCAAATCCA CACAGCCCTC GGCTGCAGAC GTTTATAGCA 
GAAGAAAAGC TAAATCTACA GAGAGTGACC AGACAAGACG GGGAAACTGG AAATGGAGCA 
GAAGAAAATA TTGTTTACCT CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981401.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837169.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA.

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CloneID	OBa228085
Clone ID Related Accession (Same CDS sequence)	XM_024981401.1
Accession Version	XM_024981401.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGAGAT TAAGAATTCG GGGTGCAGCA GATCACCACC CGGGAAGGAG 
GGTGTAAAGC GTGTGAAAAG CCTGGTGGTT CCACTGAATG AACATGAGGA CCAGGAGAGG 
CTAGATTTGG ATCGTTCCTT TGATGCCCTC AAAAAATCCA AGCACCCCTC AACATTGCTT 
GCTGCTGGCC AGCTCTCTGA CATGCTGAAT GGTGGTGATG ACGTCTACGC TAACTGCATG 
GTGATAGACC AGGTTGGTGA TTTGGACATT AACTATATTA ATATAGAGGA AATCCCTGCA 
AATACCTGTC TTGAATCCAT GGATTCTTCT CTGGGGCCTG AGAGCAGCAA ACCCAGCCTT 
CCTACTGAAG CCAGTGCTGG CACATACCCA CCAAATAAGA GGCCAGAAGT GACGTCACAC 
ACGGAAGCCC AATCATCTTT CATATCATCT AGTTCGTATG CTTCCAATTT GAATCTTTCT 
TTTGGTCTTC GTGGATTTGA AAAGGAGCAA AGTCGTCTAA AGAAAAGAAG TTCCAGCCTT 
GATGCCTTGG ATGCTGACAG TGAAGGAGAG GGCCATTCGG AGCAGTCGCA CAATTGCTAC 
ACTCCCGTGT GTCAGAGTTC CTCGAGAACT GGCATTGCTA GCGGGGATGA ATTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CGGAATCATT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCATTGTTT TCTGGAATCC GTTCACGTTC GTATTCCTGC 
TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGATGC GTGATTTTAC AGTGTGTAAT 
TCAATTGAAG AGCAAAGAGC TTACAGCTTG CCGGAGCCAC CGAGAGAACA AAGAATTCAG 
GAAGAAGAAT GGGATAAATA TATCATACCT GCCAAATCAG AATCTGAAAA ATACAAAGTA 
AGCCGAACTT TTAGCTTCCT CATGAATAGG ATGACCAGCC CTCGGAATAA ATCAAAGACA 
AAAGGCAAGG ATGCCAAAGA CAAAGAGAAA CTGACTCGAC ATCAGTTTGT CCCTGGAACT 
TTCTCTGGGG TTCTGCAGTG TTTGGTTTGC GATAAAACGC TCCTGGGGAA AGAGTCATTC 
CAGTGCTCCA ACTGTAATGG AAACGTACAT AAAGTCTGTA AAGATGCTGC CCCTCCGTGT 
ACCAAGAAAT TCCAAGACAA ATATAATAAG AACAAACCAC AGACCATCCT TGGAAATTCT 
TCGTTTCGAG ACGTCCCATT GCCTGGGCTC TCTCTGCACC CTTCCTCCTC CATGCCTATC 
GGGTTGCCAA CTGGGAGGAA GGAGATGGCA GGGCAAGTGC ATCCCTTGTC CAGAAGTGTC 
CCAGGCACCA CCTTGGAAAG CTTCAGGAGG CCGGGGGCAT CCTTGGAGTC CGAGAATGAT 
GGCAGCACCT GGAGAAGCAG GTCTCACTCT GACGAGCTGT TCCAGCCCAT GGGCTCGTCC 
CCCTCCACGG ACTCCTTCTT AACGGAAGAT GTCGTGGATT CTTCTCTGTG GAGTGACCTC 
AGCGGCGATG CCCAGGAGTT TGAAGCGGAA TCTTGGAGTC TGGTGGTGGA CCCTTCATTT 
TGTAGTCGGC AGGAGAAGGA TGTCATCCAA AGACAGGATG TCATTTTTGA GCTAATGCAG 
ACAGAACTGC ATCACATCCA GACCCTGTTC ATCATGTCTG AGATCTTCAG GAAAGGGATG 
AAAGAGGAGC TGCAGCTGGA CCACAGCACT GTGGACAAAA TCTTCCCCTG TCTGGACGAG 
TTACTTGAAA TCCACAAGCA TTTCTTCTGT AGTATGAAGG AGCGAAGGCA GGAATCCTGT 
GTTGGCAATG ACAGGAATTT CATCATCGGC CGAATTGGAG ATATTCTAGT ACAACAGTTT 
TCAGAAGAAA ATGCAAATAA AATGAAGAAA ATATATGGGG AATTCTGTAG CCATCACAAA 
GAGGCTGTTA GCCTCTTTAA AGAACTCCAG CAGAATAAAA AGTTTCAGAA TTTTATTAAG 
CTCCGAAATA GTAACCTTCT GGCTCGACGC CGAGGAATTC CAGAATGCAT TCTGCTGGTC 
ACTCAGCGCA TCACAAAATA CCCTGTCTTG GTGGAAAGGA TATTGCAGTA CACAAAGGAG 
AGCACTGAAG AACAGAGAGA CTTATGCAAA GCTCTTTGCT TAATTAAAGA CATGATTGCA 
GCTGTGGATT TAAAAGTCAG CGAATATGAG AAAAAACAAA AATGGCTTGA GATCCTAAAT 
AAGATCGAAA ACAAAACATA CACCAAGCTC AAAAATGGCC ATGTGTTTAG GAAACAGGCA 
CTGATGAGTA AAGAGAGGAC CCTGTTATAT GATGGCCTTG TCTATTGGAA AACTGCTACA 
GGTCGTTTCA AAGATATCCT AGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCAGTTAT TTCCCTTCAG 
AAGCTCATTG CTAGGGAAGT TGCGAATGAG GAGAGAGGAA TGTTTTTGAT CAGCGCTTCA 
TCTGCTGGTC CCGAGATGTA TGAAATCCAT ACCAACTCCA AGGAGGAAAG AAATACCTGG 
ATGAGACGGA TCCAACAGGC AGTAGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT 
GAGTCTGATG AAGAGAGGAG GAAAGCTGAA GCCAGAGTGG CGAAGATTCA GCAATGCCAA 
GAAATACTCA GTAACCAAGA TCAACAAATT TGCACATATT TGGAAGAGAA ACTGCATATC 
TACGCGAAAC TTGGAGAACT AAGTGGATTT CAGGATGTCC ATCTAGAGCC CCACCTCCTC 
ATTCAACCAG ACCCCGGAGA GCCTCCCCAG GCAGCCTCAT TACTGGCCGC AGCACTGAAA 
GAGGCTGAGA GTCTGCAGGT GGCAGTGAAG GCTTCCCAGA TGAGCGATGC ATGCCAGTCC 
CCCGAGGAAG GTTGTGGAGA ACCTGCTTTG CCGGACGTGC TCCATTCTCA GGACGCACCA 
GGATCGTCTA CTGCTTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG 
GATGCCGGGA CCCAGGGTGT GGGAACCGAC CTGGCGGTTT CTGATGCGGG GGAGAAGGTG 
GAATATAGAA GTTTCCCAGG TTCTTTGCAG TCAGAGATTA TACAAGCGAT ACAGAATTTA 
ACTCGTCTCT TATATAGCCT TCAGGCCGCC TTGACCATTC AGGATAGCCA CATTGAGATC 
CACAGGCTGG TTCTCCAACA GCGGGAGAGC CTGGCCCCGG GCCCCGCGTT CCGAGGCAGC 
CCCTTTCAGG ACCAGGAGAA GTCCCGCAGC CTGGAGAAGC AGCGGGAGGA GCTGGCCGAT 
ACCCACAAGC TGCAGCTCCA GTTCCAGCAA GACCAGCACC GCTGGCTCCG CAGGTGCGAC 
CAGCAGCAGC GCGAGCAGGA GGCCCGGGCC AGCCGGCTGC AGGAGCGCGA GAGGGAGTGC 
CGGGCGCAGG AGGAGCTGCT GCTCAGGAGC CGGGGCGAGC TGGACCAGCA GCTCCAAGAC 
TACCAGCAGA GCCTGGAGCG CCTGCGCGAG GGCCAGCGCC TGGTGGAGAG GGAGCGGGAG 
AGGATGCGGG CCCAGCAGAG CCTGCTGCGC CACTGGAAGC ACGGGCGGCA GAGCAGCCTT 
CCCGCCGCGT TTTCTCCAGG AGCCCTGGAG GTAATGGAAC TTAATCGATC TGAGACTTTA 
CGTCATGAAA ATTCATTCTT CATCAATGAA GCTTTAGTGC AAATGTCACT CAACCCTTTG 
AACAAACCGC CGCCACCAGG TATCCATCAG GATGCGGCTT ACCCCCCAGG CATACCTCAT 
TCTGATTTGG TAAGGACTAC TGAAAATCAA GTAGACCTCA AGACGGACGT TTCTCAACCC 
TCAGATATCA ACCATGAACT GTGGATAGCG GCTGGGGCCT GTCATCAGAT ACCCCCTCTC 
CACCAAAGCA GCAAGGATTC TTGTAAAAAT GATGTGAACA TCTCTCAGAC TGCATACCCA 
CCCCTCCATG ACTCAAATCC ACACAGCCCT CGGCTGCAGA CGTTTATAGC AGAAGAAAAG 
CTAAATCTAC AGAGAGTGAC CAGACAAGAC GGGGAAACTG GAAATGGAGC AGAAGAAAAT 
ATTGTTTACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837169.1
CDS	17..5251
Translation	MQPTLHFLPIFRCPFSCELCEEWIVSWSSLCNAEPIEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981401.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981401.1

ORF Insert Sequence:

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGAGAT TAAGAATTCG GGGTGCAGCA GATCACCACC CGGGAAGGAG 
GGTGTAAAGC GTGTGAAAAG CCTGGTGGTT CCACTGAATG AACATGAGGA CCAGGAGAGG 
CTAGATTTGG ATCGTTCCTT TGATGCCCTC AAAAAATCCA AGCACCCCTC AACATTGCTT 
GCTGCTGGCC AGCTCTCTGA CATGCTGAAT GGTGGTGATG ACGTCTACGC TAACTGCATG 
GTGATAGACC AGGTTGGTGA TTTGGACATT AACTATATTA ATATAGAGGA AATCCCTGCA 
AATACCTGTC TTGAATCCAT GGATTCTTCT CTGGGGCCTG AGAGCAGCAA ACCCAGCCTT 
CCTACTGAAG CCAGTGCTGG CACATACCCA CCAAATAAGA GGCCAGAAGT GACGTCACAC 
ACGGAAGCCC AATCATCTTT CATATCATCT AGTTCGTATG CTTCCAATTT GAATCTTTCT 
TTTGGTCTTC GTGGATTTGA AAAGGAGCAA AGTCGTCTAA AGAAAAGAAG TTCCAGCCTT 
GATGCCTTGG ATGCTGACAG TGAAGGAGAG GGCCATTCGG AGCAGTCGCA CAATTGCTAC 
ACTCCCGTGT GTCAGAGTTC CTCGAGAACT GGCATTGCTA GCGGGGATGA ATTGGACTCT 
TTTGAGACCA ACACTGAACC AGATTTTAAT ATCTCCAGGA CGGAATCATT TTCTTTATCA 
AGTAGCCTGC AGCTGAAGGA ATCATTGTTT TCTGGAATCC GTTCACGTTC GTATTCCTGC 
TCATCACCCA AAATTTCTTT AGGAAAAACT CGATTGATGC GTGATTTTAC AGTGTGTAAT 
TCAATTGAAG AGCAAAGAGC TTACAGCTTG CCGGAGCCAC CGAGAGAACA AAGAATTCAG 
GAAGAAGAAT GGGATAAATA TATCATACCT GCCAAATCAG AATCTGAAAA ATACAAAGTA 
AGCCGAACTT TTAGCTTCCT CATGAATAGG ATGACCAGCC CTCGGAATAA ATCAAAGACA 
AAAGGCAAGG ATGCCAAAGA CAAAGAGAAA CTGACTCGAC ATCAGTTTGT CCCTGGAACT 
TTCTCTGGGG TTCTGCAGTG TTTGGTTTGC GATAAAACGC TCCTGGGGAA AGAGTCATTC 
CAGTGCTCCA ACTGTAATGG AAACGTACAT AAAGTCTGTA AAGATGCTGC CCCTCCGTGT 
ACCAAGAAAT TCCAAGACAA ATATAATAAG AACAAACCAC AGACCATCCT TGGAAATTCT 
TCGTTTCGAG ACGTCCCATT GCCTGGGCTC TCTCTGCACC CTTCCTCCTC CATGCCTATC 
GGGTTGCCAA CTGGGAGGAA GGAGATGGCA GGGCAAGTGC ATCCCTTGTC CAGAAGTGTC 
CCAGGCACCA CCTTGGAAAG CTTCAGGAGG CCGGGGGCAT CCTTGGAGTC CGAGAATGAT 
GGCAGCACCT GGAGAAGCAG GTCTCACTCT GACGAGCTGT TCCAGCCCAT GGGCTCGTCC 
CCCTCCACGG ACTCCTTCTT AACGGAAGAT GTCGTGGATT CTTCTCTGTG GAGTGACCTC 
AGCGGCGATG CCCAGGAGTT TGAAGCGGAA TCTTGGAGTC TGGTGGTGGA CCCTTCATTT 
TGTAGTCGGC AGGAGAAGGA TGTCATCCAA AGACAGGATG TCATTTTTGA GCTAATGCAG 
ACAGAACTGC ATCACATCCA GACCCTGTTC ATCATGTCTG AGATCTTCAG GAAAGGGATG 
AAAGAGGAGC TGCAGCTGGA CCACAGCACT GTGGACAAAA TCTTCCCCTG TCTGGACGAG 
TTACTTGAAA TCCACAAGCA TTTCTTCTGT AGTATGAAGG AGCGAAGGCA GGAATCCTGT 
GTTGGCAATG ACAGGAATTT CATCATCGGC CGAATTGGAG ATATTCTAGT ACAACAGTTT 
TCAGAAGAAA ATGCAAATAA AATGAAGAAA ATATATGGGG AATTCTGTAG CCATCACAAA 
GAGGCTGTTA GCCTCTTTAA AGAACTCCAG CAGAATAAAA AGTTTCAGAA TTTTATTAAG 
CTCCGAAATA GTAACCTTCT GGCTCGACGC CGAGGAATTC CAGAATGCAT TCTGCTGGTC 
ACTCAGCGCA TCACAAAATA CCCTGTCTTG GTGGAAAGGA TATTGCAGTA CACAAAGGAG 
AGCACTGAAG AACAGAGAGA CTTATGCAAA GCTCTTTGCT TAATTAAAGA CATGATTGCA 
GCTGTGGATT TAAAAGTCAG CGAATATGAG AAAAAACAAA AATGGCTTGA GATCCTAAAT 
AAGATCGAAA ACAAAACATA CACCAAGCTC AAAAATGGCC ATGTGTTTAG GAAACAGGCA 
CTGATGAGTA AAGAGAGGAC CCTGTTATAT GATGGCCTTG TCTATTGGAA AACTGCTACA 
GGTCGTTTCA AAGATATCCT AGCTCTACTT CTAACTGATG TGCTGCTCTT TTTACAAGAA 
AAAGACCAGA AATACATCTT TGCAGCTGTT GATCAGAAGC CATCAGTTAT TTCCCTTCAG 
AAGCTCATTG CTAGGGAAGT TGCGAATGAG GAGAGAGGAA TGTTTTTGAT CAGCGCTTCA 
TCTGCTGGTC CCGAGATGTA TGAAATCCAT ACCAACTCCA AGGAGGAAAG AAATACCTGG 
ATGAGACGGA TCCAACAGGC AGTAGAAAGT TGCCCAGAAG AAGAAGGGGG AAGGACAAGT 
GAGTCTGATG AAGAGAGGAG GAAAGCTGAA GCCAGAGTGG CGAAGATTCA GCAATGCCAA 
GAAATACTCA GTAACCAAGA TCAACAAATT TGCACATATT TGGAAGAGAA ACTGCATATC 
TACGCGAAAC TTGGAGAACT AAGTGGATTT CAGGATGTCC ATCTAGAGCC CCACCTCCTC 
ATTCAACCAG ACCCCGGAGA GCCTCCCCAG GCAGCCTCAT TACTGGCCGC AGCACTGAAA 
GAGGCTGAGA GTCTGCAGGT GGCAGTGAAG GCTTCCCAGA TGAGCGATGC ATGCCAGTCC 
CCCGAGGAAG GTTGTGGAGA ACCTGCTTTG CCGGACGTGC TCCATTCTCA GGACGCACCA 
GGATCGTCTA CTGCTTCACT AGTCACAGAA GGGACAGGAG GAAGAGGCTG TTGGGATGTG 
GATGCCGGGA CCCAGGGTGT GGGAACCGAC CTGGCGGTTT CTGATGCGGG GGAGAAGGTG 
GAATATAGAA GTTTCCCAGG TTCTTTGCAG TCAGAGATTA TACAAGCGAT ACAGAATTTA 
ACTCGTCTCT TATATAGCCT TCAGGCCGCC TTGACCATTC AGGATAGCCA CATTGAGATC 
CACAGGCTGG TTCTCCAACA GCGGGAGAGC CTGGCCCCGG GCCCCGCGTT CCGAGGCAGC 
CCCTTTCAGG ACCAGGAGAA GTCCCGCAGC CTGGAGAAGC AGCGGGAGGA GCTGGCCGAT 
ACCCACAAGC TGCAGCTCCA GTTCCAGCAA GACCAGCACC GCTGGCTCCG CAGGTGCGAC 
CAGCAGCAGC GCGAGCAGGA GGCCCGGGCC AGCCGGCTGC AGGAGCGCGA GAGGGAGTGC 
CGGGCGCAGG AGGAGCTGCT GCTCAGGAGC CGGGGCGAGC TGGACCAGCA GCTCCAAGAC 
TACCAGCAGA GCCTGGAGCG CCTGCGCGAG GGCCAGCGCC TGGTGGAGAG GGAGCGGGAG 
AGGATGCGGG CCCAGCAGAG CCTGCTGCGC CACTGGAAGC ACGGGCGGCA GAGCAGCCTT 
CCCGCCGCGT TTTCTCCAGG AGCCCTGGAG GTAATGGAAC TTAATCGATC TGAGACTTTA 
CGTCATGAAA ATTCATTCTT CATCAATGAA GCTTTAGTGC AAATGTCACT CAACCCTTTG 
AACAAACCGC CGCCACCAGG TATCCATCAG GATGCGGCTT ACCCCCCAGG CATACCTCAT 
TCTGATTTGG TAAGGACTAC TGAAAATCAA GTAGACCTCA AGACGGACGT TTCTCAACCC 
TCAGATATCA ACCATGAACT GTGGATAGCG GCTGGGGCCT GTCATCAGAT ACCCCCTCTC 
CACCAAAGCA GCAAGGATTC TTGTAAAAAT GATGTGAACA TCTCTCAGAC TGCATACCCA 
CCCCTCCATG ACTCAAATCC ACACAGCCCT CGGCTGCAGA CGTTTATAGC AGAAGAAAAG 
CTAAATCTAC AGAGAGTGAC CAGACAAGAC GGGGAAACTG GAAATGGAGC AGAAGAAAAT 
ATTGTTTACC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981402.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837170.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA.

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CloneID	OBa228086
Clone ID Related Accession (Same CDS sequence)	XM_024981402.1
Accession Version	XM_024981402.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGATCG TTCCTTTGAT GCCCTCAAAA AATCCAAGCA CCCCTCAACA 
TTGCTTGCTG CTGGCCAGCT CTCTGACATG CTGAATGGTG GTGATGACGT CTACGCTAAC 
TGCATGGTGA TAGACCAGGT TGGTGATTTG GACATTAACT ATATTAATAT AGAGGAAATC 
CCTGCAAATA CCTGTCTTGA ATCCATGGAT TCTTCTCTGG GGCCTGAGAG CAGCAAACCC 
AGCCTTCCTA CTGAAGCCAG TGCTGGCACA TACCCACCAA ATAAGAGGCC AGAAGTGACG 
TCACACACGG AAGCCCAATC ATCTTTCATA TCATCTAGTT CGTATGCTTC CAATTTGAAT 
CTTTCTTTTG GTCTTCGTGG ATTTGAAAAG GAGCAAAGTC GTCTAAAGAA AAGAAGTTCC 
AGCCTTGATG CCTTGGATGC TGACAGTGAA GGAGAGGGCC ATTCGGAGCA GTCGCACAAT 
TGCTACACTC CCGTGTGTCA GAGTTCCTCG AGAACTGGCA TTGCTAGCGG GGATGAATTG 
GACTCTTTTG AGACCAACAC TGAACCAGAT TTTAATATCT CCAGGACGGA ATCATTTTCT 
TTATCAAGTA GCCTGCAGCT GAAGGAATCA TTGTTTTCTG GAATCCGTTC ACGTTCGTAT 
TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGATGCGTGA TTTTACAGTG 
TGTAATTCAA TTGAAGAGCA AAGAGCTTAC AGCTTGCCGG AGCCACCGAG AGAACAAAGA 
ATTCAGGAAG AAGAATGGGA TAAATATATC ATACCTGCCA AATCAGAATC TGAAAAATAC 
AAAGTAAGCC GAACTTTTAG CTTCCTCATG AATAGGATGA CCAGCCCTCG GAATAAATCA 
AAGACAAAAG GCAAGGATGC CAAAGACAAA GAGAAACTGA CTCGACATCA GTTTGTCCCT 
GGAACTTTCT CTGGGGTTCT GCAGTGTTTG GTTTGCGATA AAACGCTCCT GGGGAAAGAG 
TCATTCCAGT GCTCCAACTG TAATGGAAAC GTACATAAAG TCTGTAAAGA TGCTGCCCCT 
CCGTGTACCA AGAAATTCCA AGACAAATAT AATAAGAACA AACCACAGAC CATCCTTGGA 
AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG 
CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA 
AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG 
AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC 
TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT 
GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT 
TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA 
ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA 
GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG 
GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA 
TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA 
CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT 
CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT 
ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG 
CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA 
AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG 
ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC 
CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA 
CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT 
GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA 
CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC 
CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC 
GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT 
ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG 
ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA 
TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG 
CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC 
CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA 
CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC 
CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC 
GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG 
GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG 
AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG 
AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT 
GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA 
GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG 
GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG 
TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG 
GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC 
CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG 
CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC 
AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG 
ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC 
CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA 
CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT 
CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC 
CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA 
TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA 
GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA 
GAAAATATTG TTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837170.1
CDS	16..5136
Translation	MQPTLHFLPIFRCPFSCELCEEWIVSWSSLCNAEPIEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	XM_024981402.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981402.1

ORF Insert Sequence:

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGATCG TTCCTTTGAT GCCCTCAAAA AATCCAAGCA CCCCTCAACA 
TTGCTTGCTG CTGGCCAGCT CTCTGACATG CTGAATGGTG GTGATGACGT CTACGCTAAC 
TGCATGGTGA TAGACCAGGT TGGTGATTTG GACATTAACT ATATTAATAT AGAGGAAATC 
CCTGCAAATA CCTGTCTTGA ATCCATGGAT TCTTCTCTGG GGCCTGAGAG CAGCAAACCC 
AGCCTTCCTA CTGAAGCCAG TGCTGGCACA TACCCACCAA ATAAGAGGCC AGAAGTGACG 
TCACACACGG AAGCCCAATC ATCTTTCATA TCATCTAGTT CGTATGCTTC CAATTTGAAT 
CTTTCTTTTG GTCTTCGTGG ATTTGAAAAG GAGCAAAGTC GTCTAAAGAA AAGAAGTTCC 
AGCCTTGATG CCTTGGATGC TGACAGTGAA GGAGAGGGCC ATTCGGAGCA GTCGCACAAT 
TGCTACACTC CCGTGTGTCA GAGTTCCTCG AGAACTGGCA TTGCTAGCGG GGATGAATTG 
GACTCTTTTG AGACCAACAC TGAACCAGAT TTTAATATCT CCAGGACGGA ATCATTTTCT 
TTATCAAGTA GCCTGCAGCT GAAGGAATCA TTGTTTTCTG GAATCCGTTC ACGTTCGTAT 
TCCTGCTCAT CACCCAAAAT TTCTTTAGGA AAAACTCGAT TGATGCGTGA TTTTACAGTG 
TGTAATTCAA TTGAAGAGCA AAGAGCTTAC AGCTTGCCGG AGCCACCGAG AGAACAAAGA 
ATTCAGGAAG AAGAATGGGA TAAATATATC ATACCTGCCA AATCAGAATC TGAAAAATAC 
AAAGTAAGCC GAACTTTTAG CTTCCTCATG AATAGGATGA CCAGCCCTCG GAATAAATCA 
AAGACAAAAG GCAAGGATGC CAAAGACAAA GAGAAACTGA CTCGACATCA GTTTGTCCCT 
GGAACTTTCT CTGGGGTTCT GCAGTGTTTG GTTTGCGATA AAACGCTCCT GGGGAAAGAG 
TCATTCCAGT GCTCCAACTG TAATGGAAAC GTACATAAAG TCTGTAAAGA TGCTGCCCCT 
CCGTGTACCA AGAAATTCCA AGACAAATAT AATAAGAACA AACCACAGAC CATCCTTGGA 
AATTCTTCGT TTCGAGACGT CCCATTGCCT GGGCTCTCTC TGCACCCTTC CTCCTCCATG 
CCTATCGGGT TGCCAACTGG GAGGAAGGAG ATGGCAGGGC AAGTGCATCC CTTGTCCAGA 
AGTGTCCCAG GCACCACCTT GGAAAGCTTC AGGAGGCCGG GGGCATCCTT GGAGTCCGAG 
AATGATGGCA GCACCTGGAG AAGCAGGTCT CACTCTGACG AGCTGTTCCA GCCCATGGGC 
TCGTCCCCCT CCACGGACTC CTTCTTAACG GAAGATGTCG TGGATTCTTC TCTGTGGAGT 
GACCTCAGCG GCGATGCCCA GGAGTTTGAA GCGGAATCTT GGAGTCTGGT GGTGGACCCT 
TCATTTTGTA GTCGGCAGGA GAAGGATGTC ATCCAAAGAC AGGATGTCAT TTTTGAGCTA 
ATGCAGACAG AACTGCATCA CATCCAGACC CTGTTCATCA TGTCTGAGAT CTTCAGGAAA 
GGGATGAAAG AGGAGCTGCA GCTGGACCAC AGCACTGTGG ACAAAATCTT CCCCTGTCTG 
GACGAGTTAC TTGAAATCCA CAAGCATTTC TTCTGTAGTA TGAAGGAGCG AAGGCAGGAA 
TCCTGTGTTG GCAATGACAG GAATTTCATC ATCGGCCGAA TTGGAGATAT TCTAGTACAA 
CAGTTTTCAG AAGAAAATGC AAATAAAATG AAGAAAATAT ATGGGGAATT CTGTAGCCAT 
CACAAAGAGG CTGTTAGCCT CTTTAAAGAA CTCCAGCAGA ATAAAAAGTT TCAGAATTTT 
ATTAAGCTCC GAAATAGTAA CCTTCTGGCT CGACGCCGAG GAATTCCAGA ATGCATTCTG 
CTGGTCACTC AGCGCATCAC AAAATACCCT GTCTTGGTGG AAAGGATATT GCAGTACACA 
AAGGAGAGCA CTGAAGAACA GAGAGACTTA TGCAAAGCTC TTTGCTTAAT TAAAGACATG 
ATTGCAGCTG TGGATTTAAA AGTCAGCGAA TATGAGAAAA AACAAAAATG GCTTGAGATC 
CTAAATAAGA TCGAAAACAA AACATACACC AAGCTCAAAA ATGGCCATGT GTTTAGGAAA 
CAGGCACTGA TGAGTAAAGA GAGGACCCTG TTATATGATG GCCTTGTCTA TTGGAAAACT 
GCTACAGGTC GTTTCAAAGA TATCCTAGCT CTACTTCTAA CTGATGTGCT GCTCTTTTTA 
CAAGAAAAAG ACCAGAAATA CATCTTTGCA GCTGTTGATC AGAAGCCATC AGTTATTTCC 
CTTCAGAAGC TCATTGCTAG GGAAGTTGCG AATGAGGAGA GAGGAATGTT TTTGATCAGC 
GCTTCATCTG CTGGTCCCGA GATGTATGAA ATCCATACCA ACTCCAAGGA GGAAAGAAAT 
ACCTGGATGA GACGGATCCA ACAGGCAGTA GAAAGTTGCC CAGAAGAAGA AGGGGGAAGG 
ACAAGTGAGT CTGATGAAGA GAGGAGGAAA GCTGAAGCCA GAGTGGCGAA GATTCAGCAA 
TGCCAAGAAA TACTCAGTAA CCAAGATCAA CAAATTTGCA CATATTTGGA AGAGAAACTG 
CATATCTACG CGAAACTTGG AGAACTAAGT GGATTTCAGG ATGTCCATCT AGAGCCCCAC 
CTCCTCATTC AACCAGACCC CGGAGAGCCT CCCCAGGCAG CCTCATTACT GGCCGCAGCA 
CTGAAAGAGG CTGAGAGTCT GCAGGTGGCA GTGAAGGCTT CCCAGATGAG CGATGCATGC 
CAGTCCCCCG AGGAAGGTTG TGGAGAACCT GCTTTGCCGG ACGTGCTCCA TTCTCAGGAC 
GCACCAGGAT CGTCTACTGC TTCACTAGTC ACAGAAGGGA CAGGAGGAAG AGGCTGTTGG 
GATGTGGATG CCGGGACCCA GGGTGTGGGA ACCGACCTGG CGGTTTCTGA TGCGGGGGAG 
AAGGTGGAAT ATAGAAGTTT CCCAGGTTCT TTGCAGTCAG AGATTATACA AGCGATACAG 
AATTTAACTC GTCTCTTATA TAGCCTTCAG GCCGCCTTGA CCATTCAGGA TAGCCACATT 
GAGATCCACA GGCTGGTTCT CCAACAGCGG GAGAGCCTGG CCCCGGGCCC CGCGTTCCGA 
GGCAGCCCCT TTCAGGACCA GGAGAAGTCC CGCAGCCTGG AGAAGCAGCG GGAGGAGCTG 
GCCGATACCC ACAAGCTGCA GCTCCAGTTC CAGCAAGACC AGCACCGCTG GCTCCGCAGG 
TGCGACCAGC AGCAGCGCGA GCAGGAGGCC CGGGCCAGCC GGCTGCAGGA GCGCGAGAGG 
GAGTGCCGGG CGCAGGAGGA GCTGCTGCTC AGGAGCCGGG GCGAGCTGGA CCAGCAGCTC 
CAAGACTACC AGCAGAGCCT GGAGCGCCTG CGCGAGGGCC AGCGCCTGGT GGAGAGGGAG 
CGGGAGAGGA TGCGGGCCCA GCAGAGCCTG CTGCGCCACT GGAAGCACGG GCGGCAGAGC 
AGCCTTCCCG CCGCGTTTTC TCCAGGAGCC CTGGAGGTAA TGGAACTTAA TCGATCTGAG 
ACTTTACGTC ATGAAAATTC ATTCTTCATC AATGAAGCTT TAGTGCAAAT GTCACTCAAC 
CCTTTGAACA AACCGCCGCC ACCAGGTATC CATCAGGATG CGGCTTACCC CCCAGGCATA 
CCTCATTCTG ATTTGGTAAG GACTACTGAA AATCAAGTAG ACCTCAAGAC GGACGTTTCT 
CAACCCTCAG ATATCAACCA TGAACTGTGG ATAGCGGCTG GGGCCTGTCA TCAGATACCC 
CCTCTCCACC AAAGCAGCAA GGATTCTTGT AAAAATGATG TGAACATCTC TCAGACTGCA 
TACCCACCCC TCCATGACTC AAATCCACAC AGCCCTCGGC TGCAGACGTT TATAGCAGAA 
GAAAAGCTAA ATCTACAGAG AGTGACCAGA CAAGACGGGG AAACTGGAAA TGGAGCAGAA 
GAAAATATTG TTTACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( XM_024981403.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837171.1 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA.

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CloneID	OBa228087
Clone ID Related Accession (Same CDS sequence)	XM_024981403.1
Accession Version	XM_024981403.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4977bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG 
CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG 
GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC 
CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC 
CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT 
CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA 
GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC 
CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT 
CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG 
GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG 
TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC 
AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG 
CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA 
GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA 
TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT 
TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG 
GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC 
AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA 
TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA 
GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA 
ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC 
ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC 
TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG 
GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT 
GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG 
CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG 
CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG 
CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA 
ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT 
GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA 
AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT 
AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT 
AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC 
ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA 
GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT 
TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA 
AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT 
AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC 
AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG 
AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT 
GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT 
CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG 
ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT 
GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC 
AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA 
CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA 
GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG 
AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA 
GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT 
ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG 
ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA 
AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC 
TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG 
GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG 
GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG 
CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG 
CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG 
GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG 
AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG 
GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG 
TTTTCTCCAG GAGCCCTGGA GGTACGGGAC TCCTGTGCTC GCAGAAGCAG AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837171.1
CDS	121..5097
Translation	MQPTLHFLPIFRCPFSCELCEEWIVSWSSLCNAEPIEDAETMELNCSEVPLYGQMTVCAKFGKNVYLPKDAEFYFIYNGSHQRHIVIAERTEDNVLQSSIPGHGLQETVTVSVCLCSEGYSPVTMGSDSVTYVDNMACRLARLLVTQADRLTASSHQTLLTPFALTAGALPALDEELVLALTHLELPLGWTVLGNSSLEVSLHRESLLHLAMRWGLAKLSQLLLCLPGGAQALTLPNEEGATPLDLALHGGHSKLVEDITNFQGRRSPGFSCVQLSEDASLRYIHSSETLTLTLNHTAEHLLEADIKLFRKYFWDRAFLIKALEEQEGTSEEKPDMPSSAAETEEGMHAPSPPGELRKCSAFSSQLRVEVCGEGEQEPMDSEARVGCSGAAGPRVPSAALTKEIKNSGCSRSPPGKEGVKRVKSLVVPLNEHEDQERLDLDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVRDSCARRSRE

Target ORF information:

RefSeq Version	XM_024981403.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981403.1

ORF Insert Sequence:

ATGCAGCCCA CTCTTCACTT TCTTCCCATC TTTAGGTGTC CGTTCAGCTG TGAGCTCTGT 
GAGGAGTGGA TCGTTTCCTG GTCATCTTTG TGCAATGCTG AGCCCATTGA AGATGCGGAA 
ACCATGGAGC TGAACTGCAG CGAAGTACCT CTGTATGGGC AGATGACGGT CTGCGCGAAG 
TTTGGCAAAA ATGTTTATCT TCCTAAAGAT GCTGAGTTTT ACTTTATTTA TAATGGATCT 
CATCAGAGAC ACATCGTGAT TGCAGAGCGC ACAGAAGATA ATGTTCTCCA GTCCAGCATT 
CCAGGCCACG GGCTTCAGGA GACGGTCACC GTGTCTGTGT GCCTGTGCTC GGAAGGTTAC 
TCCCCGGTGA CCATGGGCTC TGACTCCGTG ACCTACGTGG ACAACATGGC CTGCAGGCTG 
GCCCGTCTGC TGGTCACCCA GGCCGATCGC CTCACCGCCA GCAGCCACCA GACCTTGCTG 
ACCCCGTTTG CCCTGACGGC GGGGGCATTG CCTGCTCTGG ACGAGGAGCT CGTGCTGGCC 
CTGACTCATC TGGAGTTGCC TCTGGGGTGG ACCGTCTTGG GGAATTCTTC ACTGGAAGTT 
TCTCTGCATA GGGAGTCTCT TCTGCACCTG GCTATGAGAT GGGGCCTTGC TAAGCTTTCC 
CAGCTCCTTC TGTGTCTCCC TGGGGGAGCC CAGGCCCTGA CTTTACCCAA CGAGGAGGGT 
GCCACGCCAT TAGACTTGGC TTTACATGGT GGACATTCCA AGCTGGTTGA AGACATCACA 
AACTTCCAGG GCAGGCGTTC CCCAGGTTTC TCCTGCGTGC AGCTCAGCGA AGATGCCTCT 
CTGCGGTATA TTCACTCATC AGAAACTCTG ACCCTGACGC TTAACCACAC AGCCGAGCAT 
TTGCTGGAGG CGGATATTAA ACTCTTCAGA AAATACTTTT GGGATAGAGC CTTTCTCATC 
AAGGCTCTTG AAGAGCAAGA AGGCACGTCA GAGGAGAAAC CAGATATGCC CTCCAGCGCT 
GCAGAAACTG AAGAAGGTAT GCATGCTCCT TCCCCACCTG GGGAACTCAG AAAGTGCTCA 
GCATTCTCTA GTCAATTGCG AGTTGAAGTG TGTGGAGAAG GAGAGCAGGA GCCGATGGAC 
TCGGAGGCGA GAGTAGGTTG CTCTGGGGCC GCAGGGCCCA GAGTTCCTTC AGCAGCACTG 
ACCAAAGAGA TTAAGAATTC GGGGTGCAGC AGATCACCAC CCGGGAAGGA GGGTGTAAAG 
CGTGTGAAAA GCCTGGTGGT TCCACTGAAT GAACATGAGG ACCAGGAGAG GCTAGATTTG 
GATCGTTCCT TTGATGCCCT CAAAAAATCC AAGCACCCCT CAACATTGCT TGCTGCTGGC 
CAGCTCTCTG ACATGCTGAA TGGTGGTGAT GACGTCTACG CTAACTGCAT GGTGATAGAC 
CAGGTTGGTG ATTTGGACAT TAACTATATT AATATAGAGG AAATCCCTGC AAATACCTGT 
CTTGAATCCA TGGATTCTTC TCTGGGGCCT GAGAGCAGCA AACCCAGCCT TCCTACTGAA 
GCCAGTGCTG GCACATACCC ACCAAATAAG AGGCCAGAAG TGACGTCACA CACGGAAGCC 
CAATCATCTT TCATATCATC TAGTTCGTAT GCTTCCAATT TGAATCTTTC TTTTGGTCTT 
CGTGGATTTG AAAAGGAGCA AAGTCGTCTA AAGAAAAGAA GTTCCAGCCT TGATGCCTTG 
GATGCTGACA GTGAAGGAGA GGGCCATTCG GAGCAGTCGC ACAATTGCTA CACTCCCGTG 
TGTCAGAGTT CCTCGAGAAC TGGCATTGCT AGCGGGGATG AATTGGACTC TTTTGAGACC 
AACACTGAAC CAGATTTTAA TATCTCCAGG ACGGAATCAT TTTCTTTATC AAGTAGCCTG 
CAGCTGAAGG AATCATTGTT TTCTGGAATC CGTTCACGTT CGTATTCCTG CTCATCACCC 
AAAATTTCTT TAGGAAAAAC TCGATTGATG CGTGATTTTA CAGTGTGTAA TTCAATTGAA 
GAGCAAAGAG CTTACAGCTT GCCGGAGCCA CCGAGAGAAC AAAGAATTCA GGAAGAAGAA 
TGGGATAAAT ATATCATACC TGCCAAATCA GAATCTGAAA AATACAAAGT AAGCCGAACT 
TTTAGCTTCC TCATGAATAG GATGACCAGC CCTCGGAATA AATCAAAGAC AAAAGGCAAG 
GATGCCAAAG ACAAAGAGAA ACTGACTCGA CATCAGTTTG TCCCTGGAAC TTTCTCTGGG 
GTTCTGCAGT GTTTGGTTTG CGATAAAACG CTCCTGGGGA AAGAGTCATT CCAGTGCTCC 
AACTGTAATG GAAACGTACA TAAAGTCTGT AAAGATGCTG CCCCTCCGTG TACCAAGAAA 
TTCCAAGACA AATATAATAA GAACAAACCA CAGACCATCC TTGGAAATTC TTCGTTTCGA 
GACGTCCCAT TGCCTGGGCT CTCTCTGCAC CCTTCCTCCT CCATGCCTAT CGGGTTGCCA 
ACTGGGAGGA AGGAGATGGC AGGGCAAGTG CATCCCTTGT CCAGAAGTGT CCCAGGCACC 
ACCTTGGAAA GCTTCAGGAG GCCGGGGGCA TCCTTGGAGT CCGAGAATGA TGGCAGCACC 
TGGAGAAGCA GGTCTCACTC TGACGAGCTG TTCCAGCCCA TGGGCTCGTC CCCCTCCACG 
GACTCCTTCT TAACGGAAGA TGTCGTGGAT TCTTCTCTGT GGAGTGACCT CAGCGGCGAT 
GCCCAGGAGT TTGAAGCGGA ATCTTGGAGT CTGGTGGTGG ACCCTTCATT TTGTAGTCGG 
CAGGAGAAGG ATGTCATCCA AAGACAGGAT GTCATTTTTG AGCTAATGCA GACAGAACTG 
CATCACATCC AGACCCTGTT CATCATGTCT GAGATCTTCA GGAAAGGGAT GAAAGAGGAG 
CTGCAGCTGG ACCACAGCAC TGTGGACAAA ATCTTCCCCT GTCTGGACGA GTTACTTGAA 
ATCCACAAGC ATTTCTTCTG TAGTATGAAG GAGCGAAGGC AGGAATCCTG TGTTGGCAAT 
GACAGGAATT TCATCATCGG CCGAATTGGA GATATTCTAG TACAACAGTT TTCAGAAGAA 
AATGCAAATA AAATGAAGAA AATATATGGG GAATTCTGTA GCCATCACAA AGAGGCTGTT 
AGCCTCTTTA AAGAACTCCA GCAGAATAAA AAGTTTCAGA ATTTTATTAA GCTCCGAAAT 
AGTAACCTTC TGGCTCGACG CCGAGGAATT CCAGAATGCA TTCTGCTGGT CACTCAGCGC 
ATCACAAAAT ACCCTGTCTT GGTGGAAAGG ATATTGCAGT ACACAAAGGA GAGCACTGAA 
GAACAGAGAG ACTTATGCAA AGCTCTTTGC TTAATTAAAG ACATGATTGC AGCTGTGGAT 
TTAAAAGTCA GCGAATATGA GAAAAAACAA AAATGGCTTG AGATCCTAAA TAAGATCGAA 
AACAAAACAT ACACCAAGCT CAAAAATGGC CATGTGTTTA GGAAACAGGC ACTGATGAGT 
AAAGAGAGGA CCCTGTTATA TGATGGCCTT GTCTATTGGA AAACTGCTAC AGGTCGTTTC 
AAAGATATCC TAGCTCTACT TCTAACTGAT GTGCTGCTCT TTTTACAAGA AAAAGACCAG 
AAATACATCT TTGCAGCTGT TGATCAGAAG CCATCAGTTA TTTCCCTTCA GAAGCTCATT 
GCTAGGGAAG TTGCGAATGA GGAGAGAGGA ATGTTTTTGA TCAGCGCTTC ATCTGCTGGT 
CCCGAGATGT ATGAAATCCA TACCAACTCC AAGGAGGAAA GAAATACCTG GATGAGACGG 
ATCCAACAGG CAGTAGAAAG TTGCCCAGAA GAAGAAGGGG GAAGGACAAG TGAGTCTGAT 
GAAGAGAGGA GGAAAGCTGA AGCCAGAGTG GCGAAGATTC AGCAATGCCA AGAAATACTC 
AGTAACCAAG ATCAACAAAT TTGCACATAT TTGGAAGAGA AACTGCATAT CTACGCGAAA 
CTTGGAGAAC TAAGTGGATT TCAGGATGTC CATCTAGAGC CCCACCTCCT CATTCAACCA 
GACCCCGGAG AGCCTCCCCA GGCAGCCTCA TTACTGGCCG CAGCACTGAA AGAGGCTGAG 
AGTCTGCAGG TGGCAGTGAA GGCTTCCCAG ATGAGCGATG CATGCCAGTC CCCCGAGGAA 
GGTTGTGGAG AACCTGCTTT GCCGGACGTG CTCCATTCTC AGGACGCACC AGGATCGTCT 
ACTGCTTCAC TAGTCACAGA AGGGACAGGA GGAAGAGGCT GTTGGGATGT GGATGCCGGG 
ACCCAGGGTG TGGGAACCGA CCTGGCGGTT TCTGATGCGG GGGAGAAGGT GGAATATAGA 
AGTTTCCCAG GTTCTTTGCA GTCAGAGATT ATACAAGCGA TACAGAATTT AACTCGTCTC 
TTATATAGCC TTCAGGCCGC CTTGACCATT CAGGATAGCC ACATTGAGAT CCACAGGCTG 
GTTCTCCAAC AGCGGGAGAG CCTGGCCCCG GGCCCCGCGT TCCGAGGCAG CCCCTTTCAG 
GACCAGGAGA AGTCCCGCAG CCTGGAGAAG CAGCGGGAGG AGCTGGCCGA TACCCACAAG 
CTGCAGCTCC AGTTCCAGCA AGACCAGCAC CGCTGGCTCC GCAGGTGCGA CCAGCAGCAG 
CGCGAGCAGG AGGCCCGGGC CAGCCGGCTG CAGGAGCGCG AGAGGGAGTG CCGGGCGCAG 
GAGGAGCTGC TGCTCAGGAG CCGGGGCGAG CTGGACCAGC AGCTCCAAGA CTACCAGCAG 
AGCCTGGAGC GCCTGCGCGA GGGCCAGCGC CTGGTGGAGA GGGAGCGGGA GAGGATGCGG 
GCCCAGCAGA GCCTGCTGCG CCACTGGAAG CACGGGCGGC AGAGCAGCCT TCCCGCCGCG 
TTTTCTCCAG GAGCCCTGGA GGTACGGGAC TCCTGTGCTC GCAGAAGCAG AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF28 ( NM_001102307.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095777.2 Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), mRNA.

Price & Availability↑

CloneID	OBa39032
Clone ID Related Accession (Same CDS sequence)	NM_001102307.1
Accession Version	NM_001102307.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4170bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 28
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC149175.1. On Aug 21, 2007 this sequence version replaced XR_028065.1. ##Evidence-Data-START## Transcript exon combination :: BC149175.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTCCC CAAAATGAAG 
ATTCGACGGA GGGCGTCCAA ACAAGATCGT TCCTTTGATG CCCTCAAAAA ATCCAAGCAC 
CCCTCAACAT TGCTTGCTGC TGGCCAGCTC TCTGACATGC TGAATGGTGG TGATGACGTC 
TACGCTAACT GCATGGTGAT AGACCAGGTT GGTGATTTGG ACATTAACTA TATTAATATA 
GAGGAAATCC CTGCAAATAC CTGTCTTGAA TCCATGGATT CTTCTCTGGG GCCTGAGAGC 
AGCAAACCCA GCCTTCCTAC TGAAGCCAGT GCTGGCACAT ACCCACCAAA TAAGAGGCCA 
GAAGTGACGT CACACACGGA AGCCCAATCA TCTTTCATAT CATCTAGTTC GTATGCTTCC 
AATTTGAATC TTTCTTTTGG TCTTCGTGGA TTTGAAAAGG AGCAAAGTCG TCTAAAGAAA 
AGAAGTTCCA GCCTTGATGC CTTGGATGCT GACAGTGAAG GAGAGGGCCA TTCGGAGCAG 
TCGCACAATT GCTACACTCC CGTGTGTCAG AGTTCCTCGA GAACTGGCAT TGCTAGCGGG 
GATGAATTGG ACTCTTTTGA GACCAACACT GAACCAGATT TTAATATCTC CAGGACGGAA 
TCATTTTCTT TATCAAGTAG CCTGCAGCTG AAGGAATCAT TGTTTTCTGG AATCCGTTCA 
CGTTCGTATT CCTGCTCATC ACCCAAAATT TCTTTAGGAA AAACTCGATT GATGCGTGAT 
TTTACAGTGT GTAATTCAAT TGAAGAGCAA AGAGCTTACA GCTTGCCGGA GCCACCGAGA 
GAACAAAGAA TTCAGGAAGA AGAATGGGAT AAATATATCA TACCTGCCAA ATCAGAATCT 
GAAAAATACA AAGTAAGCCG AACTTTTAGC TTCCTCATGA ATAGGATGAC CAGCCCTCGG 
AATAAATCAA AGACAAAAGG CAAGGATGCC AAAGACAAAG AGAAACTGAC TCGACATCAG 
TTTGTCCCTG GAACTTTCTC TGGGGTTCTG CAGTGTTTGG TTTGCGATAA AACGCTCCTG 
GGGAAAGAGT CATTCCAGTG CTCCAACTGT AATGGAAACG TACATAAAGT CTGTAAAGAT 
GCTGCCCCTC CGTGTACCAA GAAATTCCAA GACAAATATA ATAAGAACAA ACCACAGACC 
ATCCTTGGAA ATTCTTCGTT TCGAGACGTC CCATTGCCTG GGCTCTCTCT GCACCCTTCC 
TCCTCCATGC CTATCGGGTT GCCAACTGGG AGGAAGGAGA TGGCAGGGCA AGTGCATCCC 
TTGTCCAGAA GTGTCCCAGG CACCACCTTG GAAAGCTTCA GGAGGCCGGG GGCATCCTTG 
GAGTCCGAGA ATGATGGCAG CACCTGGAGA AGCAGGTCTC ACTCTGACGA GCTGTTCCAG 
CCCATGGGCT CGTCCCCCTC CACGGACTCC TTCTTAACGG AAGATGTCGT GGATTCTTCT 
CTGTGGAGTG ACCTCAGCGG CGATGCCCAG GAGTTTGAAG CGGAATCTTG GAGTCTGGTG 
GTGGACCCTT CATTTTGTAG TCGGCAGGAG AAGGATGTCA TCCAAAGACA GGATGTCATT 
TTTGAGCTAA TGCAGACAGA ACTGCATCAC ATCCAGACCC TGTTCATCAT GTCTGAGATC 
TTCAGGAAAG GGATGAAAGA GGAGCTGCAG CTGGACCACA GCACTGTGGA CAAAATCTTC 
CCCTGTCTGG ACGAGTTACT TGAAATCCAC AAGCATTTCT TCTGTAGTAT GAAGGAGCGA 
AGGCAGGAAT CCTGTGTTGG CAATGACAGG AATTTCATCA TCGGCCGAAT TGGAGATATT 
CTAGTACAAC AGTTTTCAGA AGAAAATGCA AATAAAATGA AGAAAATATA TGGGGAATTC 
TGTAGCCATC ACAAAGAGGC TGTTAGCCTC TTTAAAGAAC TCCAGCAGAA TAAAAAGTTT 
CAGAATTTTA TTAAGCTCCG AAATAGTAAC CTTCTGGCTC GACGCCGAGG AATTCCAGAA 
TGCATTCTGC TGGTCACTCA GCGCATCACA AAATACCCTG TCTTGGTGGA AAGGATATTG 
CAGTACACAA AGGAGAGCAC TGAAGAACAG AGAGACTTAT GCAAAGCTCT TTGCTTAATT 
AAAGACATGA TTGCAGCTGT GGATTTAAAA GTCAGCGAAT ATGAGAAAAA ACAAAAATGG 
CTTGAGATCC TAAATAAGAT CGAAAACAAA ACATACACCA AGCTCAAAAA TGGCCATGTG 
TTTAGGAAAC AGGCACTGAT GAGTAAAGAG AGGACCCTGT TATATGATGG CCTTGTCTAT 
TGGAAAACTG CTACAGGTCG TTTCAAAGAT ATCCTAGCTC TACTTCTAAC TGATGTGCTG 
CTCTTTTTAC AAGAAAAAGA CCAGAAATAC ATCTTTGCAG CTGTTGATCA GAAGCCATCA 
GTTATTTCCC TTCAGAAGCT CATTGCTAGG GAAGTTGCGA ATGAGGAGAG AGGAATGTTT 
TTGATCAGCG CTTCATCTGC TGGTCCCGAG ATGTATGAAA TCCATACCAA CTCTAAGGAG 
GAAAGAAATA CCTGGATGAG ACGGATCCAA CAGGCAGTAG AAAGTTGCCC AGAAGAAGAA 
GGGGGAAGGA CAAGTGAGTC TGATGAAGAG AGGAGGAAAG CTGAAGCCAG AGTGGCGAAG 
ATTCAGCAAT GCCAAGAAAT ACTCAGTAAC CAAGATCAAC AAATTTGCAC ATATTTGGAA 
GAGAAACTGC ATATCTACGC GAAACTTGGA GAACTAAGTG GATTTCAGGA TGTCCATCTA 
GAGCCCCACC TCCTCATTCA ACCAGACCCC GGAGAGCCTC CCCAGGCAGC CTCATTACTG 
GCCGCAGCAC TGAAAGAGGC TGAGAGTCTG CAGGTGGCAG TGAAGGCTTC CCAGATGAGC 
GATGCATGCC AGTCCCCCGA GGAAGGTTGT GGAGAACCTG CTTTGCCGGA CGTGCTCCAT 
TCTCAGGACG CACCAGGATC GTCTACTGCT TCACTAGTCA CAGAAGGGAC AGGAGGAAGA 
GGCTGTTGGG ATGTGGATGC CGGGACCCAG GGTGTGGGAA CCGACCTGGC GGTTTCTGAT 
GCGGGGGAGA AGGTGGAATA TAGAAGTTTC CCAGGTTCTT TGCAGTCAGA GATTATACAA 
GCGATACAGA ATTTAACTCG TCTCTTATAT AGCCTTCAGG CCGCCTTGAC CATTCAGGAT 
AGCCACATTG AGATCCACAG GCTGGTTCTC CAACAGCGGG AGAGCCTGGC CCCGGGCCCC 
GCGTTCCGAG GCAGCCCCTT TCAGGACCAG GAGAAGTCCC GCAGCCTGGA GAAGCAGCGG 
GAGGAGCTGG CCGATACCCA CAAGCTGCAG CTCCAGTTCC AGCAAGACCA GCACCGCTGG 
CTCCGCAGGT GCGACCAGCA GCAGCGCGAG CAGGAGGCCC GGGCCAGCCG GCTGCAGGAG 
CGCGAGAGGG AGTGCCGGGC GCAGGAGGAG CTGCTGCTCA GGAGCCGGGG CGAGCTGGAC 
CAGCAGCTCC AAGACTACCA GCAGAGCCTG GAGCGCCTGC GCGAGGGCCA GCGCCTGGTG 
GAGAGGGAGC GGGAGAGGAT GCGGGCCCAG CAGAGCCTGC TGCGCCACTG GAAGCACGGG 
CGGCAGAGCA GCCTTCCCGC CGCGTTTTCT CCAGGAGCCC TGGAGGTAAT GGAACTTAAT 
CGATCTGAGA CTTTACGTCA TGAAAATTCA TTCTTCATCA ATGAAGCTTT AGTGCAAATG 
TCACTCAACC CTTTGAACAA ACCGCCGCCA CCAGGTATCC ATCAGGATGC GGCTTACCCC 
CCAGGCATAC CTCATTCTGA TTTGGTAAGG ACTACTGAAA ATCAAGTAGA CCTCAAGACG 
GACGTTTCTC AACCCTCAGA TATCAACCAT GAACTGTGGA TAGCGGCTGG GGCCTGTCAT 
CAGATACCCC CTCTCCACCA AAGCAGCAAG GATTCTTGTA AAAATGATGT GAACATCTCT 
CAGACTGCAT ACCCACCCCT CCATGACTCA AATCCACACA GCCCTCGGCT GCAGACGTTT 
ATAGCAGAAG AAAAGCTAAA TCTACAGAGA GTGACCAGAC AAGACGGGGA AACTGGAAAT 
GGAGCAGAAG AAAATATTGT TTACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095777.2
CDS	21..4190
Translation	MDSDSDSPFNYSWPSFPKMKIRRRASKQDRSFDALKKSKHPSTLLAAGQLSDMLNGGDDVYANCMVIDQVGDLDINYINIEEIPANTCLESMDSSLGPESSKPSLPTEASAGTYPPNKRPEVTSHTEAQSSFISSSSYASNLNLSFGLRGFEKEQSRLKKRSSSLDALDADSEGEGHSEQSHNCYTPVCQSSSRTGIASGDELDSFETNTEPDFNISRTESFSLSSSLQLKESLFSGIRSRSYSCSSPKISLGKTRLMRDFTVCNSIEEQRAYSLPEPPREQRIQEEEWDKYIIPAKSESEKYKVSRTFSFLMNRMTSPRNKSKTKGKDAKDKEKLTRHQFVPGTFSGVLQCLVCDKTLLGKESFQCSNCNGNVHKVCKDAAPPCTKKFQDKYNKNKPQTILGNSSFRDVPLPGLSLHPSSSMPIGLPTGRKEMAGQVHPLSRSVPGTTLESFRRPGASLESENDGSTWRSRSHSDELFQPMGSSPSTDSFLTEDVVDSSLWSDLSGDAQEFEAESWSLVVDPSFCSRQEKDVIQRQDVIFELMQTELHHIQTLFIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKHFFCSMKERRQESCVGNDRNFIIGRIGDILVQQFSEENANKMKKIYGEFCSHHKEAVSLFKELQQNKKFQNFIKLRNSNLLARRRGIPECILLVTQRITKYPVLVERILQYTKESTEEQRDLCKALCLIKDMIAAVDLKVSEYEKKQKWLEILNKIENKTYTKLKNGHVFRKQALMSKERTLLYDGLVYWKTATGRFKDILALLLTDVLLFLQEKDQKYIFAAVDQKPSVISLQKLIAREVANEERGMFLISASSAGPEMYEIHTNSKEERNTWMRRIQQAVESCPEEEGGRTSESDEERRKAEARVAKIQQCQEILSNQDQQICTYLEEKLHIYAKLGELSGFQDVHLEPHLLIQPDPGEPPQAASLLAAALKEAESLQVAVKASQMSDACQSPEEGCGEPALPDVLHSQDAPGSSTASLVTEGTGGRGCWDVDAGTQGVGTDLAVSDAGEKVEYRSFPGSLQSEIIQAIQNLTRLLYSLQAALTIQDSHIEIHRLVLQQRESLAPGPAFRGSPFQDQEKSRSLEKQREELADTHKLQLQFQQDQHRWLRRCDQQQREQEARASRLQERERECRAQEELLLRSRGELDQQLQDYQQSLERLREGQRLVERERERMRAQQSLLRHWKHGRQSSLPAAFSPGALEVMELNRSETLRHENSFFINEALVQMSLNPLNKPPPPGIHQDAAYPPGIPHSDLVRTTENQVDLKTDVSQPSDINHELWIAAGACHQIPPLHQSSKDSCKNDVNISQTAYPPLHDSNPHSPRLQTFIAEEKLNLQRVTRQDGETGNGAEENIVYL

Target ORF information:

RefSeq Version	NM_001102307.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 28 (ARHGEF28), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102307.1

ORF Insert Sequence:

ATGGATTCTG ACTCGGACTC ACCTTTTAAC TACTCGTGGC CTTCCTTCCC CAAAATGAAG 
ATTCGACGGA GGGCGTCCAA ACAAGATCGT TCCTTTGATG CCCTCAAAAA ATCCAAGCAC 
CCCTCAACAT TGCTTGCTGC TGGCCAGCTC TCTGACATGC TGAATGGTGG TGATGACGTC 
TACGCTAACT GCATGGTGAT AGACCAGGTT GGTGATTTGG ACATTAACTA TATTAATATA 
GAGGAAATCC CTGCAAATAC CTGTCTTGAA TCCATGGATT CTTCTCTGGG GCCTGAGAGC 
AGCAAACCCA GCCTTCCTAC TGAAGCCAGT GCTGGCACAT ACCCACCAAA TAAGAGGCCA 
GAAGTGACGT CACACACGGA AGCCCAATCA TCTTTCATAT CATCTAGTTC GTATGCTTCC 
AATTTGAATC TTTCTTTTGG TCTTCGTGGA TTTGAAAAGG AGCAAAGTCG TCTAAAGAAA 
AGAAGTTCCA GCCTTGATGC CTTGGATGCT GACAGTGAAG GAGAGGGCCA TTCGGAGCAG 
TCGCACAATT GCTACACTCC CGTGTGTCAG AGTTCCTCGA GAACTGGCAT TGCTAGCGGG 
GATGAATTGG ACTCTTTTGA GACCAACACT GAACCAGATT TTAATATCTC CAGGACGGAA 
TCATTTTCTT TATCAAGTAG CCTGCAGCTG AAGGAATCAT TGTTTTCTGG AATCCGTTCA 
CGTTCGTATT CCTGCTCATC ACCCAAAATT TCTTTAGGAA AAACTCGATT GATGCGTGAT 
TTTACAGTGT GTAATTCAAT TGAAGAGCAA AGAGCTTACA GCTTGCCGGA GCCACCGAGA 
GAACAAAGAA TTCAGGAAGA AGAATGGGAT AAATATATCA TACCTGCCAA ATCAGAATCT 
GAAAAATACA AAGTAAGCCG AACTTTTAGC TTCCTCATGA ATAGGATGAC CAGCCCTCGG 
AATAAATCAA AGACAAAAGG CAAGGATGCC AAAGACAAAG AGAAACTGAC TCGACATCAG 
TTTGTCCCTG GAACTTTCTC TGGGGTTCTG CAGTGTTTGG TTTGCGATAA AACGCTCCTG 
GGGAAAGAGT CATTCCAGTG CTCCAACTGT AATGGAAACG TACATAAAGT CTGTAAAGAT 
GCTGCCCCTC CGTGTACCAA GAAATTCCAA GACAAATATA ATAAGAACAA ACCACAGACC 
ATCCTTGGAA ATTCTTCGTT TCGAGACGTC CCATTGCCTG GGCTCTCTCT GCACCCTTCC 
TCCTCCATGC CTATCGGGTT GCCAACTGGG AGGAAGGAGA TGGCAGGGCA AGTGCATCCC 
TTGTCCAGAA GTGTCCCAGG CACCACCTTG GAAAGCTTCA GGAGGCCGGG GGCATCCTTG 
GAGTCCGAGA ATGATGGCAG CACCTGGAGA AGCAGGTCTC ACTCTGACGA GCTGTTCCAG 
CCCATGGGCT CGTCCCCCTC CACGGACTCC TTCTTAACGG AAGATGTCGT GGATTCTTCT 
CTGTGGAGTG ACCTCAGCGG CGATGCCCAG GAGTTTGAAG CGGAATCTTG GAGTCTGGTG 
GTGGACCCTT CATTTTGTAG TCGGCAGGAG AAGGATGTCA TCCAAAGACA GGATGTCATT 
TTTGAGCTAA TGCAGACAGA ACTGCATCAC ATCCAGACCC TGTTCATCAT GTCTGAGATC 
TTCAGGAAAG GGATGAAAGA GGAGCTGCAG CTGGACCACA GCACTGTGGA CAAAATCTTC 
CCCTGTCTGG ACGAGTTACT TGAAATCCAC AAGCATTTCT TCTGTAGTAT GAAGGAGCGA 
AGGCAGGAAT CCTGTGTTGG CAATGACAGG AATTTCATCA TCGGCCGAAT TGGAGATATT 
CTAGTACAAC AGTTTTCAGA AGAAAATGCA AATAAAATGA AGAAAATATA TGGGGAATTC 
TGTAGCCATC ACAAAGAGGC TGTTAGCCTC TTTAAAGAAC TCCAGCAGAA TAAAAAGTTT 
CAGAATTTTA TTAAGCTCCG AAATAGTAAC CTTCTGGCTC GACGCCGAGG AATTCCAGAA 
TGCATTCTGC TGGTCACTCA GCGCATCACA AAATACCCTG TCTTGGTGGA AAGGATATTG 
CAGTACACAA AGGAGAGCAC TGAAGAACAG AGAGACTTAT GCAAAGCTCT TTGCTTAATT 
AAAGACATGA TTGCAGCTGT GGATTTAAAA GTCAGCGAAT ATGAGAAAAA ACAAAAATGG 
CTTGAGATCC TAAATAAGAT CGAAAACAAA ACATACACCA AGCTCAAAAA TGGCCATGTG 
TTTAGGAAAC AGGCACTGAT GAGTAAAGAG AGGACCCTGT TATATGATGG CCTTGTCTAT 
TGGAAAACTG CTACAGGTCG TTTCAAAGAT ATCCTAGCTC TACTTCTAAC TGATGTGCTG 
CTCTTTTTAC AAGAAAAAGA CCAGAAATAC ATCTTTGCAG CTGTTGATCA GAAGCCATCA 
GTTATTTCCC TTCAGAAGCT CATTGCTAGG GAAGTTGCGA ATGAGGAGAG AGGAATGTTT 
TTGATCAGCG CTTCATCTGC TGGTCCCGAG ATGTATGAAA TCCATACCAA CTCTAAGGAG 
GAAAGAAATA CCTGGATGAG ACGGATCCAA CAGGCAGTAG AAAGTTGCCC AGAAGAAGAA 
GGGGGAAGGA CAAGTGAGTC TGATGAAGAG AGGAGGAAAG CTGAAGCCAG AGTGGCGAAG 
ATTCAGCAAT GCCAAGAAAT ACTCAGTAAC CAAGATCAAC AAATTTGCAC ATATTTGGAA 
GAGAAACTGC ATATCTACGC GAAACTTGGA GAACTAAGTG GATTTCAGGA TGTCCATCTA 
GAGCCCCACC TCCTCATTCA ACCAGACCCC GGAGAGCCTC CCCAGGCAGC CTCATTACTG 
GCCGCAGCAC TGAAAGAGGC TGAGAGTCTG CAGGTGGCAG TGAAGGCTTC CCAGATGAGC 
GATGCATGCC AGTCCCCCGA GGAAGGTTGT GGAGAACCTG CTTTGCCGGA CGTGCTCCAT 
TCTCAGGACG CACCAGGATC GTCTACTGCT TCACTAGTCA CAGAAGGGAC AGGAGGAAGA 
GGCTGTTGGG ATGTGGATGC CGGGACCCAG GGTGTGGGAA CCGACCTGGC GGTTTCTGAT 
GCGGGGGAGA AGGTGGAATA TAGAAGTTTC CCAGGTTCTT TGCAGTCAGA GATTATACAA 
GCGATACAGA ATTTAACTCG TCTCTTATAT AGCCTTCAGG CCGCCTTGAC CATTCAGGAT 
AGCCACATTG AGATCCACAG GCTGGTTCTC CAACAGCGGG AGAGCCTGGC CCCGGGCCCC 
GCGTTCCGAG GCAGCCCCTT TCAGGACCAG GAGAAGTCCC GCAGCCTGGA GAAGCAGCGG 
GAGGAGCTGG CCGATACCCA CAAGCTGCAG CTCCAGTTCC AGCAAGACCA GCACCGCTGG 
CTCCGCAGGT GCGACCAGCA GCAGCGCGAG CAGGAGGCCC GGGCCAGCCG GCTGCAGGAG 
CGCGAGAGGG AGTGCCGGGC GCAGGAGGAG CTGCTGCTCA GGAGCCGGGG CGAGCTGGAC 
CAGCAGCTCC AAGACTACCA GCAGAGCCTG GAGCGCCTGC GCGAGGGCCA GCGCCTGGTG 
GAGAGGGAGC GGGAGAGGAT GCGGGCCCAG CAGAGCCTGC TGCGCCACTG GAAGCACGGG 
CGGCAGAGCA GCCTTCCCGC CGCGTTTTCT CCAGGAGCCC TGGAGGTAAT GGAACTTAAT 
CGATCTGAGA CTTTACGTCA TGAAAATTCA TTCTTCATCA ATGAAGCTTT AGTGCAAATG 
TCACTCAACC CTTTGAACAA ACCGCCGCCA CCAGGTATCC ATCAGGATGC GGCTTACCCC 
CCAGGCATAC CTCATTCTGA TTTGGTAAGG ACTACTGAAA ATCAAGTAGA CCTCAAGACG 
GACGTTTCTC AACCCTCAGA TATCAACCAT GAACTGTGGA TAGCGGCTGG GGCCTGTCAT 
CAGATACCCC CTCTCCACCA AAGCAGCAAG GATTCTTGTA AAAATGATGT GAACATCTCT 
CAGACTGCAT ACCCACCCCT CCATGACTCA AATCCACACA GCCCTCGGCT GCAGACGTTT 
ATAGCAGAAG AAAAGCTAAA TCTACAGAGA GTGACCAGAC AAGACGGGGA AACTGGAAAT 
GGAGCAGAAG AAAATATTGT TTACCTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ARHGEF28
Entrez Gene ID	616969
Full Name	Rho guanine nucleotide exchange factor 28
Synonyms	RGNEF
General protein information	Preferred Names Rho guanine nucleotide exchange factor 28 Names rho guanine nucleotide exchange factor 28 190 kDa guanine nucleotide exchange factor Rho guanine nucleotide exchange factor (GEF) 28 Rho-guanine nucleotide exchange factor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	ARHGEF28	XP_001151239.2
Rattus norvegicus (Norway rat)	Arhgef28	NP_001102012.1
Gallus gallus (chicken)	LOC427201	XP_424789.4
Danio rerio (zebrafish)	arhgef28	XP_688428.4
Homo sapiens (human)	ARHGEF28	NP_001073948.2
Bos taurus (cattle)	RGNEF	NP_001095777.2
	ARHGEF28	XP_001101425.1
Canis lupus familiaris (dog)	ARHGEF28	XP_005617529.1
Mus musculus (house mouse)	Arhgef28	NP_036156.2
Xenopus tropicalis (tropical clawed frog)	arhgef28	XP_002934885.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ARHGEF28 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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