arhgef28 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_002934839.4	XP_002934885.2	rho guanine nucleotide exchange factor 28 isoform X1
XM_004910823.3	XP_004910880.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_004910823.4	XP_004910880.1	rho guanine nucleotide exchange factor 28 isoform X2
XM_012952887.3	XP_012808341.1	rho guanine nucleotide exchange factor 28 isoform X3
XM_031893732.1	XP_031749592.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_002934839.5	XP_002934885.2	rho guanine nucleotide exchange factor 28 isoform X1
XM_012952887.2	XP_012808341.1	rho guanine nucleotide exchange factor 28 isoform X3
XM_012952889.2	XP_012808343.1	rho guanine nucleotide exchange factor 28 isoform X5
XM_018090771.1	XP_017946260.1	rho guanine nucleotide exchange factor 28 isoform X4
XM_012952889.3	XP_012808343.1	rho guanine nucleotide exchange factor 28 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa35296	XM_012952889.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa42210	XM_018090771.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OXa49100	XM_031893732.1 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa04015	XM_002934839.5 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa04016	XM_004910823.4 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OXa35295	XM_012952887.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
OXa04016	XM_004910823.3	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa04015	XM_002934839.4	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OXa35295	XM_012952887.2	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OXa35296	XM_012952889.2	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

arhgef28 ( XM_012952889.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808343.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa35296
Clone ID Related Accession (Same CDS sequence)	XM_012952889.2 , XM_012952889.3
Accession Version	XM_012952889.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG 
TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT 
GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC 
ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG 
ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA 
AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT 
GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT 
GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA 
ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA 
AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA 
GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT 
TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC 
TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA 
GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC 
CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT 
CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC 
AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG 
AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG 
AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA 
GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG 
AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA 
CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC 
ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT 
GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA 
GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA 
GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA 
TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA 
CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT 
ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG 
GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC 
CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA 
ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC 
TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG 
AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG 
GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG 
GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC 
CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA 
GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG 
AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG 
GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA 
ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC 
CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG 
CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC 
AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT 
CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC 
AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT 
AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT 
GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG 
GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA 
CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT 
TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA 
AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT 
CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA 
GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT 
ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT 
AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC 
CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC 
TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG 
CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT 
GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT 
GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC 
CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA 
GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA 
ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT 
GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC 
TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT 
AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT 
GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA 
AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808343.1
CDS	181..4356
Translation	MESDSDSPFNYSWPSFPKMRIHRRSSKQDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_012952889.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952889.3

ORF Insert Sequence:

ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG 
TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT 
GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC 
ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG 
ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA 
AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT 
GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT 
GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA 
ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA 
AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA 
GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT 
TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC 
TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA 
GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC 
CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT 
CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC 
AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG 
AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG 
AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA 
GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG 
AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA 
CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC 
ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT 
GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA 
GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA 
GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA 
TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA 
CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT 
ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG 
GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC 
CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA 
ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC 
TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG 
AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG 
GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG 
GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC 
CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA 
GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG 
AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG 
GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA 
ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC 
CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG 
CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC 
AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT 
CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC 
AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT 
AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT 
GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG 
GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA 
CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT 
TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA 
AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT 
CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA 
GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT 
ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT 
AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC 
CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC 
TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG 
CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT 
GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT 
GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC 
CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA 
GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA 
ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT 
GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC 
TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT 
AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT 
GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA 
AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_018090771.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017946260.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa42210
Clone ID Related Accession (Same CDS sequence)	XM_018090771.1
Accession Version	XM_018090771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG 
GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA 
ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG 
TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG 
GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT 
TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA 
TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC 
CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG 
AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC 
CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT 
TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC 
CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA 
TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA 
AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG 
AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG 
CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA 
TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT 
TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG 
CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA 
GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT 
TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA 
GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT 
GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG 
GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG 
GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG 
GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG 
GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT 
CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA 
TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG 
AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA 
GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT AAGTAGCCCT 
GTGTTCCTAA CATTTTGTGG CAAGTTAAAG CTGCTTGCTG TGCACTCAGT TAGCCACAAA 
CATATGCTTT ATATTTGTAT GTCTGCATGC ACCGACTCTG CCAGCTCACT TGTCATAAAG 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017946260.1
CDS	1083..5288
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVSSPVFLTFCGKLKLLAVHSVSHKHMLYICMSACTDSASSLVIKI

Target ORF information:

RefSeq Version	XM_018090771.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018090771.1

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG 
GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA 
ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG 
TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG 
GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT 
TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA 
TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC 
CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG 
AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC 
CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT 
TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC 
CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA 
TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA 
AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG 
AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG 
CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA 
TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT 
TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG 
CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA 
GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT 
TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA 
GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT 
GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG 
GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG 
GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG 
GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG 
GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT 
CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA 
TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG 
AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA 
GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT AAGTAGCCCT 
GTGTTCCTAA CATTTTGTGG CAAGTTAAAG CTGCTTGCTG TGCACTCAGT TAGCCACAAA 
CATATGCTTT ATATTTGTAT GTCTGCATGC ACCGACTCTG CCAGCTCACT TGTCATAAAG 
ATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_031893732.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031749592.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.

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CloneID	OXa49100
Clone ID Related Accession (Same CDS sequence)	XM_031893732.1
Accession Version	XM_031893732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG 
TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT 
GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC 
ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG 
ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA 
AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT 
GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT 
GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA 
ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA 
AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA 
GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT 
TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC 
TCCGTCAGTG ACACAAGTGA GGAACAGCGG GCGTATAGCC TCCCTGATCA ACTGAGAGAA 
AAAAGGATTG AGGAAGAGGA ATGGGATAAA TACGCAACTC CTACAAAACC AGAATCTGAA 
AAGAACAAAG TCAGCCGGAC ATTTAGCTTC TTGCGGAACA GGATGACCAG CACCAGGAAC 
AAAAACAAGG TGAAAAATAA GGATATGAAA GAGAAGGTGA ACAGGCATCA GTTTGTGACG 
GGCACGTTCT CTGGGGTGGT TCCATGTTTG GTGTGTGAGA AGGCACTCCT GGGGAAGGAG 
TCTCTGCAGT GCTCCAGTTG TAATGTGAAT GTACACAAAG GCTGCAAGGA ATCTGCTCCC 
CATTGCACCA AGCGATTTCA GGAACGATAT CACCCAAAGA ACAAGCAGTC GGCAGTTATA 
TCAAATTCAT CATATAAAGA TATCCCACAA CAAGTTCAAC CTACAATGCA TACTTCTACA 
TCTGTGCCTC TGGGTCTCTC CTCGGGCAAG AAAGAGACCA TTCACTCCAC TCCCCTGCAT 
TCTAAAACCA TCCCTGTGTT AAGCTCTGAT AGAAGGTCTG ACCCCTCTCT GGAATCAGAT 
GGGGACACCA GTGGCTGCAG ATCGAGATCT CAGTCGGAAG AGCTGCTGCA AACCTTGGGC 
ACACCTCCAT CCATGGACTG CTTCCCAATT GAAGATGTAG TAGATACAAC GTTATGGAGT 
GACTTCAGTA ATGACATGCA GGAATTCGAA GCCGAGTCAT GGAGTCTTGT GGTTGATCCA 
TTATTCTGTG AAAAGCAAAA GAAAGATGTT ATAAAGAGAC AAGATGTCAT TTTTGAACTG 
ATCCAGACAG AACTCCATCA CGTCCAGACA TTGTTGATTA TGTCTGAGAT ATTCAGAAAA 
GGAATGAAAG AGGAGCTTCA GTTGGACCAC AGCACAGTGG ATAAAATATT CCCTTGTCTG 
GATGAGCTGC TGGAGATCCA CAAGCAGTTC TTCTCCAACC TTAAAGAAAG GAAACAGGAA 
TCTAGGGAAG GAAACGACAA AAACTTTATC ATCAACAGAA TTGGAGATAT CCTCGTGCAA 
CAGTTTTCAG CAGACAGTGC CAATAAAATG AAGAAGATCT ATGGAGAGTT TTGCAGTCAC 
CATATCGAAG CACTGAACTT GTTTAAGGAG CTACAGCAGA ACAAGAAATT CCAGAACTTT 
ATTAAACTAA GGAACCGTAA TCTCCTGGCA CGCCGACGGG GTATCCCGGA ATGCATCCTT 
CTTGTAACTC AGCGCATCAC AAAGTATCCA GTGCTTGTGG AAAGGATATT AAAATACACC 
ACCGAAGGGA CAGAGGAGCA CACAGATCTC TGCAAAGCCC TGGTGTTAAT GAAGGAGCTG 
CTGGCAGCAG TGGATTTGCA GGTCAATAAG TTTGAGAAAG GACAAAAGCT GCTAGAGATT 
CTGAATAAAA TTGAAAATAA AACTTGCACC AAATTAAAGA ATGGCCTAGA ATTCAGAAAG 
CAGGACCTGA CGAGCAAAGA GCGGGCGCTG CTGCATGAGG GAGTGGTGTT TTGGAAAACG 
GCTACCGGGC GCTTTAAAGA TATTCAAGCA CTTCTGTTAA CGGACATCCT GCTGTTTCTA 
CAAGAGAAGG ATCAAAAGTA TATTTTTGCT GCAGTGGACC AGAAACCCCC AGTTATTTGC 
CTTCGGAAAC TGATTGTGCG AGAAGTAGCG AATGAGGAGC GCGGGATGTT TTTAATAAGT 
GCCTCATCCG CTGGGCCTGA AATGTATGAG ATCCACACCA ATTCCAAGGA AGAGCGCAAT 
GGCTGGATGA GGCAGATTCA GGAGGCTGTA CAGAGCTGTC CGCAGGAAGA TGAAGGTAAA 
AGCTGTGAAT CAGATGAAGA TAAACGGGTG GCAGAGGCCA GAGCAGTGAA GGCTCAAAAG 
TATCATGATA CGCTGACCCT CCAAGATCAG AATATCTGTA ATACTTTAGA GGAAAAATTA 
CAGATCTACG CAGAACTTGC AGCCTTAAAC ACAAAGAGTG ACATGCACTT GGAACCTCAC 
CTTTTTATCA AAGCCGATTC AGGGGAAATC CCTCAGGCGG CGGCTCTGCT GGCTGCTGCA 
CTCAAGGAAG CTGAAAATCT CCACTCAACC ATTAAATCAC AAATGGAGGA TCCATCTTGG 
TTACCTGAAT ATGAAAGAAG CACTCCGAGA AGATGTCGTT CTGTTGAAAC ATTAGATGAG 
GAACCGGAGA CATCATATAA CTCCCAGAGC CCAGATGCAA GGGAGAGAAG GAAGCTTTCC 
TATGATGCCA GTATTTCTCA TGTGAAGAGT GACAGGGATC TGGATTTCAC AGAAGAAATT 
GAGGAATGTA TGCAAAGTCC AAGCTATAAT CAGGATGAAG TTCTACAAGT GGTGCATAAC 
CTTACTCGCC TGCTGTACAG CATACAGGCT ACTGTTGCTA TGCAAGATAG CCACATAGAA 
CTGCATAAAG CTTTCCTACA GGATTATGAA TTGGGCAGTA GAGGTTCCCG TGGAAGTATA 
CTTAATGAAC ATGAGAAGTA TCGCAACCTA GAAAAGCACC GCTGCGAGAT GGTCAATTTA 
CATAAACTCC AGCAACAGTT GCATCAAGAA CAACAACGCT GGCTGCGCGA GTGCGACCAA 
AAACAGAGGG AAAACGAGGA GAAGGAGAGC ATGCTCCAGC ATAGGGAGCA AGAGTGTCAG 
AGTCAGGAAC ATCTCTTGCA CCAAAAGAGG GAGGAACTTG ACCAACAGCA GCAAGAATTC 
CAGCAAAACG TGGAGCGGCT ACAGGAGGGT CATAGACTTG TGGAGAGGGA GAAGGAGGAT 
CTGGCTGTGA AACTTAAGCT TTGGAGGCAC TTTAACAACC ATCAGCCAAG GAGTCTATTG 
ACTGAGGGTG TCGGAAGCCG TCGCTCTGTC AGTTTACAAG ACGAAGACTC TGTGTATATT 
AACAGTGCCT TGGCTCAGAT ATCACTCAAC ACCATCACAA CATTTCCAGC CCCAGACTAC 
CCGAATGCAC ATTTGTTTCA CAGCCCCAGT TCACTCCTTG CACAGTCTAC TGAGAAAAAC 
ACAACGGACA CTGCTAATGA GCCTTGGAGC TCTGTGGTCT CTCGCCACCA AAGACTTGAA 
GGATCTTCAC TCATTTCCCG GCACAACAAG GATGCATGTA ACAATGATCT GAATGCAGCT 
CATGTTATTT CCTGTGGTAC GCTCCTGTCT GTGCCTAATG CAACGCAGCC TGTGGAACCA 
CAGACCAAAC CCGCAGAAGA AACCTCACAT CATTTGGAAA ATGGCAAAAT TGAAGAGAAC 
ATAGTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031749592.1
CDS	181..4275
Translation	MESDSDSPFNYSWPSFPKMRIHRRSSKQDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEEQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_031893732.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031893732.1

ORF Insert Sequence:

ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG 
TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT 
GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC 
ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG 
ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA 
AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT 
GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT 
GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA 
ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA 
AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA 
GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT 
TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC 
TCCGTCAGTG ACACAAGTGA GGAACAGCGG GCGTATAGCC TCCCTGATCA ACTGAGAGAA 
AAAAGGATTG AGGAAGAGGA ATGGGATAAA TACGCAACTC CTACAAAACC AGAATCTGAA 
AAGAACAAAG TCAGCCGGAC ATTTAGCTTC TTGCGGAACA GGATGACCAG CACCAGGAAC 
AAAAACAAGG TGAAAAATAA GGATATGAAA GAGAAGGTGA ACAGGCATCA GTTTGTGACG 
GGCACGTTCT CTGGGGTGGT TCCATGTTTG GTGTGTGAGA AGGCACTCCT GGGGAAGGAG 
TCTCTGCAGT GCTCCAGTTG TAATGTGAAT GTACACAAAG GCTGCAAGGA ATCTGCTCCC 
CATTGCACCA AGCGATTTCA GGAACGATAT CACCCAAAGA ACAAGCAGTC GGCAGTTATA 
TCAAATTCAT CATATAAAGA TATCCCACAA CAAGTTCAAC CTACAATGCA TACTTCTACA 
TCTGTGCCTC TGGGTCTCTC CTCGGGCAAG AAAGAGACCA TTCACTCCAC TCCCCTGCAT 
TCTAAAACCA TCCCTGTGTT AAGCTCTGAT AGAAGGTCTG ACCCCTCTCT GGAATCAGAT 
GGGGACACCA GTGGCTGCAG ATCGAGATCT CAGTCGGAAG AGCTGCTGCA AACCTTGGGC 
ACACCTCCAT CCATGGACTG CTTCCCAATT GAAGATGTAG TAGATACAAC GTTATGGAGT 
GACTTCAGTA ATGACATGCA GGAATTCGAA GCCGAGTCAT GGAGTCTTGT GGTTGATCCA 
TTATTCTGTG AAAAGCAAAA GAAAGATGTT ATAAAGAGAC AAGATGTCAT TTTTGAACTG 
ATCCAGACAG AACTCCATCA CGTCCAGACA TTGTTGATTA TGTCTGAGAT ATTCAGAAAA 
GGAATGAAAG AGGAGCTTCA GTTGGACCAC AGCACAGTGG ATAAAATATT CCCTTGTCTG 
GATGAGCTGC TGGAGATCCA CAAGCAGTTC TTCTCCAACC TTAAAGAAAG GAAACAGGAA 
TCTAGGGAAG GAAACGACAA AAACTTTATC ATCAACAGAA TTGGAGATAT CCTCGTGCAA 
CAGTTTTCAG CAGACAGTGC CAATAAAATG AAGAAGATCT ATGGAGAGTT TTGCAGTCAC 
CATATCGAAG CACTGAACTT GTTTAAGGAG CTACAGCAGA ACAAGAAATT CCAGAACTTT 
ATTAAACTAA GGAACCGTAA TCTCCTGGCA CGCCGACGGG GTATCCCGGA ATGCATCCTT 
CTTGTAACTC AGCGCATCAC AAAGTATCCA GTGCTTGTGG AAAGGATATT AAAATACACC 
ACCGAAGGGA CAGAGGAGCA CACAGATCTC TGCAAAGCCC TGGTGTTAAT GAAGGAGCTG 
CTGGCAGCAG TGGATTTGCA GGTCAATAAG TTTGAGAAAG GACAAAAGCT GCTAGAGATT 
CTGAATAAAA TTGAAAATAA AACTTGCACC AAATTAAAGA ATGGCCTAGA ATTCAGAAAG 
CAGGACCTGA CGAGCAAAGA GCGGGCGCTG CTGCATGAGG GAGTGGTGTT TTGGAAAACG 
GCTACCGGGC GCTTTAAAGA TATTCAAGCA CTTCTGTTAA CGGACATCCT GCTGTTTCTA 
CAAGAGAAGG ATCAAAAGTA TATTTTTGCT GCAGTGGACC AGAAACCCCC AGTTATTTGC 
CTTCGGAAAC TGATTGTGCG AGAAGTAGCG AATGAGGAGC GCGGGATGTT TTTAATAAGT 
GCCTCATCCG CTGGGCCTGA AATGTATGAG ATCCACACCA ATTCCAAGGA AGAGCGCAAT 
GGCTGGATGA GGCAGATTCA GGAGGCTGTA CAGAGCTGTC CGCAGGAAGA TGAAGGTAAA 
AGCTGTGAAT CAGATGAAGA TAAACGGGTG GCAGAGGCCA GAGCAGTGAA GGCTCAAAAG 
TATCATGATA CGCTGACCCT CCAAGATCAG AATATCTGTA ATACTTTAGA GGAAAAATTA 
CAGATCTACG CAGAACTTGC AGCCTTAAAC ACAAAGAGTG ACATGCACTT GGAACCTCAC 
CTTTTTATCA AAGCCGATTC AGGGGAAATC CCTCAGGCGG CGGCTCTGCT GGCTGCTGCA 
CTCAAGGAAG CTGAAAATCT CCACTCAACC ATTAAATCAC AAATGGAGGA TCCATCTTGG 
TTACCTGAAT ATGAAAGAAG CACTCCGAGA AGATGTCGTT CTGTTGAAAC ATTAGATGAG 
GAACCGGAGA CATCATATAA CTCCCAGAGC CCAGATGCAA GGGAGAGAAG GAAGCTTTCC 
TATGATGCCA GTATTTCTCA TGTGAAGAGT GACAGGGATC TGGATTTCAC AGAAGAAATT 
GAGGAATGTA TGCAAAGTCC AAGCTATAAT CAGGATGAAG TTCTACAAGT GGTGCATAAC 
CTTACTCGCC TGCTGTACAG CATACAGGCT ACTGTTGCTA TGCAAGATAG CCACATAGAA 
CTGCATAAAG CTTTCCTACA GGATTATGAA TTGGGCAGTA GAGGTTCCCG TGGAAGTATA 
CTTAATGAAC ATGAGAAGTA TCGCAACCTA GAAAAGCACC GCTGCGAGAT GGTCAATTTA 
CATAAACTCC AGCAACAGTT GCATCAAGAA CAACAACGCT GGCTGCGCGA GTGCGACCAA 
AAACAGAGGG AAAACGAGGA GAAGGAGAGC ATGCTCCAGC ATAGGGAGCA AGAGTGTCAG 
AGTCAGGAAC ATCTCTTGCA CCAAAAGAGG GAGGAACTTG ACCAACAGCA GCAAGAATTC 
CAGCAAAACG TGGAGCGGCT ACAGGAGGGT CATAGACTTG TGGAGAGGGA GAAGGAGGAT 
CTGGCTGTGA AACTTAAGCT TTGGAGGCAC TTTAACAACC ATCAGCCAAG GAGTCTATTG 
ACTGAGGGTG TCGGAAGCCG TCGCTCTGTC AGTTTACAAG ACGAAGACTC TGTGTATATT 
AACAGTGCCT TGGCTCAGAT ATCACTCAAC ACCATCACAA CATTTCCAGC CCCAGACTAC 
CCGAATGCAC ATTTGTTTCA CAGCCCCAGT TCACTCCTTG CACAGTCTAC TGAGAAAAAC 
ACAACGGACA CTGCTAATGA GCCTTGGAGC TCTGTGGTCT CTCGCCACCA AAGACTTGAA 
GGATCTTCAC TCATTTCCCG GCACAACAAG GATGCATGTA ACAATGATCT GAATGCAGCT 
CATGTTATTT CCTGTGGTAC GCTCCTGTCT GTGCCTAATG CAACGCAGCC TGTGGAACCA 
CAGACCAAAC CCGCAGAAGA AACCTCACAT CATTTGGAAA ATGGCAAAAT TGAAGAGAAC 
ATAGTTTACC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_002934839.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934885.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.

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CloneID	OXa04015
Clone ID Related Accession (Same CDS sequence)	XM_002934839.4 , XM_002934839.5
Accession Version	XM_002934839.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934839.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG 
GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA 
ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG 
TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG 
GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT 
TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA 
TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC 
CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG 
AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC 
CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT 
TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC 
CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA 
TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA 
AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG 
AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG 
CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA 
TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT 
TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG 
CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA 
GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT 
TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA 
GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT 
GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG 
GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG 
GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG 
GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG 
GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT 
CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA 
TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG 
AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA 
GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG 
GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC 
CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT 
GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG 
GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG 
TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA 
GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG 
CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG 
AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG 
AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT 
GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC 
CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT 
GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA 
AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG 
AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT 
GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT 
GAAGAGAACA TAGTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934885.2
CDS	1071..6074
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_002934839.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934839.5

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG 
GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA 
ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG 
TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG 
GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT 
TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA 
TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC 
CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG 
AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC 
CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT 
TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC 
CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA 
TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA 
AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG 
AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG 
CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA 
TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT 
TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG 
CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA 
GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT 
TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA 
GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT 
GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG 
GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG 
GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG 
GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG 
GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT 
CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA 
TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG 
AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA 
GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG 
GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC 
CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT 
GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG 
GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG 
TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA 
GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG 
CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG 
AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG 
AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT 
GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC 
CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT 
GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA 
AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG 
AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT 
GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT 
GAAGAGAACA TAGTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_004910823.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910880.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.

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CloneID	OXa04016
Clone ID Related Accession (Same CDS sequence)	XM_004910823.3 , XM_004910823.4
Accession Version	XM_004910823.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910823.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA 
TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC 
TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA 
TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT 
GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT 
GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC 
AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT 
TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA 
CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC 
GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC 
AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG 
AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC 
ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA 
TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG 
GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA 
GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC 
AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG 
AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG 
TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT 
ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA 
ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG 
CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA 
GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT 
CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA 
AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA 
CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT 
GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA 
TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA 
GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG 
CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA 
GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG 
CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC 
ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA 
AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC 
AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC 
GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG 
TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA 
GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG 
GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT 
CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG 
TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA 
GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG 
AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA 
CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT 
GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG 
GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA 
GAGAACATAG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910880.1
CDS	1072..6072
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_004910823.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910823.4

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA 
TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC 
TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA 
TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT 
GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT 
GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC 
AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT 
TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA 
CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC 
GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC 
AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG 
AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC 
ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA 
TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG 
GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA 
GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC 
AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG 
AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG 
TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT 
ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA 
ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG 
CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA 
GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT 
CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA 
AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA 
CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT 
GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA 
TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA 
GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG 
CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA 
GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG 
CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC 
ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA 
AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC 
AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC 
GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG 
TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA 
GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG 
GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT 
CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG 
TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA 
GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG 
AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA 
CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT 
GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG 
GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA 
GAGAACATAG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_012952887.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808341.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa35295
Clone ID Related Accession (Same CDS sequence)	XM_012952887.3 , XM_012952887.2
Accession Version	XM_012952887.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952887.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG 
GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC 
AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG 
AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT 
GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC 
TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG 
CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA 
TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG 
GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC 
CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT 
GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC 
ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT 
GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA 
AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA 
CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG 
GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG 
GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA 
AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA 
GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA 
CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG 
AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG 
CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA 
GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG 
GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT 
GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG 
AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC 
TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT 
CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG 
ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT 
GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG 
CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA 
GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG 
CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA 
GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA 
GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT 
GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT 
GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA 
TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT 
ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG 
CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG 
CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT 
TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT 
GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG 
CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA 
AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT 
CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG 
GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA 
CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC 
GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG 
GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT 
TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT 
GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC 
ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC 
TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC 
GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808341.1
CDS	1071..5993
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEEQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_012952887.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952887.3

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG 
GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC 
AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG 
AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT 
GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC 
TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG 
CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA 
TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG 
GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC 
CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT 
GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC 
ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT 
GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA 
AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA 
CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG 
GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG 
GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA 
AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA 
GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA 
CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG 
AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG 
CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA 
GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG 
GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT 
GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG 
AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC 
TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT 
CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG 
ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT 
GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG 
CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA 
GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG 
CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA 
GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA 
GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT 
GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT 
GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA 
TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT 
ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG 
CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG 
CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT 
TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT 
GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG 
CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA 
AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT 
CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG 
GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA 
CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC 
GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG 
GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT 
TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT 
GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC 
ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC 
TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC 
GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_002934839.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934885.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.

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CloneID	OXa04015
Clone ID Related Accession (Same CDS sequence)	XM_002934839.4 , XM_002934839.5
Accession Version	XM_002934839.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934839.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG 
GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA 
ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG 
TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG 
GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT 
TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA 
TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC 
CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG 
AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC 
CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT 
TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC 
CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA 
TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA 
AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG 
AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG 
CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA 
TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT 
TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG 
CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA 
GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT 
TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA 
GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT 
GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG 
GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG 
GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG 
GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG 
GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT 
CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA 
TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG 
AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA 
GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG 
GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC 
CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT 
GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG 
GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG 
TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA 
GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG 
CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG 
AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG 
AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT 
GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC 
CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT 
GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA 
AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG 
AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT 
GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT 
GAAGAGAACA TAGTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934885.2
CDS	1081..6084
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_002934839.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934839.4

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GAAGGTCTGA CCCCTCTCTG 
GAATCAGATG GGGACACCAG TGGCTGCAGA TCGAGATCTC AGTCGGAAGA GCTGCTGCAA 
ACCTTGGGCA CACCTCCATC CATGGACTGC TTCCCAATTG AAGATGTAGT AGATACAACG 
TTATGGAGTG ACTTCAGTAA TGACATGCAG GAATTCGAAG CCGAGTCATG GAGTCTTGTG 
GTTGATCCAT TATTCTGTGA AAAGCAAAAG AAAGATGTTA TAAAGAGACA AGATGTCATT 
TTTGAACTGA TCCAGACAGA ACTCCATCAC GTCCAGACAT TGTTGATTAT GTCTGAGATA 
TTCAGAAAAG GAATGAAAGA GGAGCTTCAG TTGGACCACA GCACAGTGGA TAAAATATTC 
CCTTGTCTGG ATGAGCTGCT GGAGATCCAC AAGCAGTTCT TCTCCAACCT TAAAGAAAGG 
AAACAGGAAT CTAGGGAAGG AAACGACAAA AACTTTATCA TCAACAGAAT TGGAGATATC 
CTCGTGCAAC AGTTTTCAGC AGACAGTGCC AATAAAATGA AGAAGATCTA TGGAGAGTTT 
TGCAGTCACC ATATCGAAGC ACTGAACTTG TTTAAGGAGC TACAGCAGAA CAAGAAATTC 
CAGAACTTTA TTAAACTAAG GAACCGTAAT CTCCTGGCAC GCCGACGGGG TATCCCGGAA 
TGCATCCTTC TTGTAACTCA GCGCATCACA AAGTATCCAG TGCTTGTGGA AAGGATATTA 
AAATACACCA CCGAAGGGAC AGAGGAGCAC ACAGATCTCT GCAAAGCCCT GGTGTTAATG 
AAGGAGCTGC TGGCAGCAGT GGATTTGCAG GTCAATAAGT TTGAGAAAGG ACAAAAGCTG 
CTAGAGATTC TGAATAAAAT TGAAAATAAA ACTTGCACCA AATTAAAGAA TGGCCTAGAA 
TTCAGAAAGC AGGACCTGAC GAGCAAAGAG CGGGCGCTGC TGCATGAGGG AGTGGTGTTT 
TGGAAAACGG CTACCGGGCG CTTTAAAGAT ATTCAAGCAC TTCTGTTAAC GGACATCCTG 
CTGTTTCTAC AAGAGAAGGA TCAAAAGTAT ATTTTTGCTG CAGTGGACCA GAAACCCCCA 
GTTATTTGCC TTCGGAAACT GATTGTGCGA GAAGTAGCGA ATGAGGAGCG CGGGATGTTT 
TTAATAAGTG CCTCATCCGC TGGGCCTGAA ATGTATGAGA TCCACACCAA TTCCAAGGAA 
GAGCGCAATG GCTGGATGAG GCAGATTCAG GAGGCTGTAC AGAGCTGTCC GCAGGAAGAT 
GAAGGTAAAA GCTGTGAATC AGATGAAGAT AAACGGGTGG CAGAGGCCAG AGCAGTGAAG 
GCTCAAAAGT ATCATGATAC GCTGACCCTC CAAGATCAGA ATATCTGTAA TACTTTAGAG 
GAAAAATTAC AGATCTACGC AGAACTTGCA GCCTTAAACA CAAAGAGTGA CATGCACTTG 
GAACCTCACC TTTTTATCAA AGCCGATTCA GGGGAAATCC CTCAGGCGGC GGCTCTGCTG 
GCTGCTGCAC TCAAGGAAGC TGAAAATCTC CACTCAACCA TTAAATCACA AATGGAGGAT 
CCATCTTGGT TACCTGAATA TGAAAGAAGC ACTCCGAGAA GATGTCGTTC TGTTGAAACA 
TTAGATGAGG AACCGGAGAC ATCATATAAC TCCCAGAGCC CAGATGCAAG GGAGAGAAGG 
AAGCTTTCCT ATGATGCCAG TATTTCTCAT GTGAAGAGTG ACAGGGATCT GGATTTCACA 
GAAGAAATTG AGGAATGTAT GCAAAGTCCA AGCTATAATC AGGATGAAGT TCTACAAGTG 
GTGCATAACC TTACTCGCCT GCTGTACAGC ATACAGGCTA CTGTTGCTAT GCAAGATAGC 
CACATAGAAC TGCATAAAGC TTTCCTACAG GATTATGAAT TGGGCAGTAG AGGTTCCCGT 
GGAAGTATAC TTAATGAACA TGAGAAGTAT CGCAACCTAG AAAAGCACCG CTGCGAGATG 
GTCAATTTAC ATAAACTCCA GCAACAGTTG CATCAAGAAC AACAACGCTG GCTGCGCGAG 
TGCGACCAAA AACAGAGGGA AAACGAGGAG AAGGAGAGCA TGCTCCAGCA TAGGGAGCAA 
GAGTGTCAGA GTCAGGAACA TCTCTTGCAC CAAAAGAGGG AGGAACTTGA CCAACAGCAG 
CAAGAATTCC AGCAAAACGT GGAGCGGCTA CAGGAGGGTC ATAGACTTGT GGAGAGGGAG 
AAGGAGGATC TGGCTGTGAA ACTTAAGCTT TGGAGGCACT TTAACAACCA TCAGCCAAGG 
AGTCTATTGA CTGAGGGTGT CGGAAGCCGT CGCTCTGTCA GTTTACAAGA CGAAGACTCT 
GTGTATATTA ACAGTGCCTT GGCTCAGATA TCACTCAACA CCATCACAAC ATTTCCAGCC 
CCAGACTACC CGAATGCACA TTTGTTTCAC AGCCCCAGTT CACTCCTTGC ACAGTCTACT 
GAGAAAAACA CAACGGACAC TGCTAATGAG CCTTGGAGCT CTGTGGTCTC TCGCCACCAA 
AGACTTGAAG GATCTTCACT CATTTCCCGG CACAACAAGG ATGCATGTAA CAATGATCTG 
AATGCAGCTC ATGTTATTTC CTGTGGTACG CTCCTGTCTG TGCCTAATGC AACGCAGCCT 
GTGGAACCAC AGACCAAACC CGCAGAAGAA ACCTCACATC ATTTGGAAAA TGGCAAAATT 
GAAGAGAACA TAGTTTACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_004910823.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004910880.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.

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CloneID	OXa04016
Clone ID Related Accession (Same CDS sequence)	XM_004910823.3 , XM_004910823.4
Accession Version	XM_004910823.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5001bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910823.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA 
TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC 
TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA 
TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT 
GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT 
GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC 
AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT 
TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA 
CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC 
GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC 
AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG 
AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC 
ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA 
TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG 
GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA 
GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC 
AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG 
AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG 
TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT 
ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA 
ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG 
CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA 
GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT 
CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA 
AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA 
CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT 
GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA 
TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA 
GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG 
CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA 
GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG 
CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC 
ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA 
AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC 
AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC 
GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG 
TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA 
GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG 
GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT 
CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG 
TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA 
GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG 
AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA 
CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT 
GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG 
GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA 
GAGAACATAG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004910880.1
CDS	1081..6081
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_004910823.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004910823.3

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG ATGGTGTGGT CAGCTTCAGT GGTCGCTCAC TCCTCCAAGC ACTTTCATTG 
TCTAAATCAG TATATCTTCT TCACCCTGGT AAACAGCGGG CGTATAGCCT CCCTGATCAA 
CTGAGAGAAA AAAGGATTGA GGAAGAGGAA TGGGATAAAT ACGCAACTCC TACAAAACCA 
GAATCTGAAA AGAACAAAGT CAGCCGGACA TTTAGCTTCT TGCGGAACAG GATGACCAGC 
ACCAGGAACA AAAACAAGGT GAAAAATAAG GATATGAAAG AGAAGGTGAA CAGGCATCAG 
TTTGTGACGG GCACGTTCTC TGGGGTGGTT CCATGTTTGG TGTGTGAGAA GGCACTCCTG 
GGGAAGGAGT CTCTGCAGTG CTCCAGTTGT AATGTGAATG TACACAAAGG CTGCAAGGAA 
TCTGCTCCCC ATTGCACCAA GCGATTTCAG GAACGATATC ACCCAAAGAA CAAGCAGTCG 
GCAGTTATAT CAAATTCATC ATATAAAGAT ATCCCACAAC AAGTTCAACC TACAATGCAT 
ACTTCTACAT CTGTGCCTCT GGGTCTCTCC TCGGGCAAGA AAGAGACCAT TCACTCCACT 
CCCCTGCATT CTAAAACCAT CCCTGTGTTA AGCTCTGATA GGTCTGACCC CTCTCTGGAA 
TCAGATGGGG ACACCAGTGG CTGCAGATCG AGATCTCAGT CGGAAGAGCT GCTGCAAACC 
TTGGGCACAC CTCCATCCAT GGACTGCTTC CCAATTGAAG ATGTAGTAGA TACAACGTTA 
TGGAGTGACT TCAGTAATGA CATGCAGGAA TTCGAAGCCG AGTCATGGAG TCTTGTGGTT 
GATCCATTAT TCTGTGAAAA GCAAAAGAAA GATGTTATAA AGAGACAAGA TGTCATTTTT 
GAACTGATCC AGACAGAACT CCATCACGTC CAGACATTGT TGATTATGTC TGAGATATTC 
AGAAAAGGAA TGAAAGAGGA GCTTCAGTTG GACCACAGCA CAGTGGATAA AATATTCCCT 
TGTCTGGATG AGCTGCTGGA GATCCACAAG CAGTTCTTCT CCAACCTTAA AGAAAGGAAA 
CAGGAATCTA GGGAAGGAAA CGACAAAAAC TTTATCATCA ACAGAATTGG AGATATCCTC 
GTGCAACAGT TTTCAGCAGA CAGTGCCAAT AAAATGAAGA AGATCTATGG AGAGTTTTGC 
AGTCACCATA TCGAAGCACT GAACTTGTTT AAGGAGCTAC AGCAGAACAA GAAATTCCAG 
AACTTTATTA AACTAAGGAA CCGTAATCTC CTGGCACGCC GACGGGGTAT CCCGGAATGC 
ATCCTTCTTG TAACTCAGCG CATCACAAAG TATCCAGTGC TTGTGGAAAG GATATTAAAA 
TACACCACCG AAGGGACAGA GGAGCACACA GATCTCTGCA AAGCCCTGGT GTTAATGAAG 
GAGCTGCTGG CAGCAGTGGA TTTGCAGGTC AATAAGTTTG AGAAAGGACA AAAGCTGCTA 
GAGATTCTGA ATAAAATTGA AAATAAAACT TGCACCAAAT TAAAGAATGG CCTAGAATTC 
AGAAAGCAGG ACCTGACGAG CAAAGAGCGG GCGCTGCTGC ATGAGGGAGT GGTGTTTTGG 
AAAACGGCTA CCGGGCGCTT TAAAGATATT CAAGCACTTC TGTTAACGGA CATCCTGCTG 
TTTCTACAAG AGAAGGATCA AAAGTATATT TTTGCTGCAG TGGACCAGAA ACCCCCAGTT 
ATTTGCCTTC GGAAACTGAT TGTGCGAGAA GTAGCGAATG AGGAGCGCGG GATGTTTTTA 
ATAAGTGCCT CATCCGCTGG GCCTGAAATG TATGAGATCC ACACCAATTC CAAGGAAGAG 
CGCAATGGCT GGATGAGGCA GATTCAGGAG GCTGTACAGA GCTGTCCGCA GGAAGATGAA 
GGTAAAAGCT GTGAATCAGA TGAAGATAAA CGGGTGGCAG AGGCCAGAGC AGTGAAGGCT 
CAAAAGTATC ATGATACGCT GACCCTCCAA GATCAGAATA TCTGTAATAC TTTAGAGGAA 
AAATTACAGA TCTACGCAGA ACTTGCAGCC TTAAACACAA AGAGTGACAT GCACTTGGAA 
CCTCACCTTT TTATCAAAGC CGATTCAGGG GAAATCCCTC AGGCGGCGGC TCTGCTGGCT 
GCTGCACTCA AGGAAGCTGA AAATCTCCAC TCAACCATTA AATCACAAAT GGAGGATCCA 
TCTTGGTTAC CTGAATATGA AAGAAGCACT CCGAGAAGAT GTCGTTCTGT TGAAACATTA 
GATGAGGAAC CGGAGACATC ATATAACTCC CAGAGCCCAG ATGCAAGGGA GAGAAGGAAG 
CTTTCCTATG ATGCCAGTAT TTCTCATGTG AAGAGTGACA GGGATCTGGA TTTCACAGAA 
GAAATTGAGG AATGTATGCA AAGTCCAAGC TATAATCAGG ATGAAGTTCT ACAAGTGGTG 
CATAACCTTA CTCGCCTGCT GTACAGCATA CAGGCTACTG TTGCTATGCA AGATAGCCAC 
ATAGAACTGC ATAAAGCTTT CCTACAGGAT TATGAATTGG GCAGTAGAGG TTCCCGTGGA 
AGTATACTTA ATGAACATGA GAAGTATCGC AACCTAGAAA AGCACCGCTG CGAGATGGTC 
AATTTACATA AACTCCAGCA ACAGTTGCAT CAAGAACAAC AACGCTGGCT GCGCGAGTGC 
GACCAAAAAC AGAGGGAAAA CGAGGAGAAG GAGAGCATGC TCCAGCATAG GGAGCAAGAG 
TGTCAGAGTC AGGAACATCT CTTGCACCAA AAGAGGGAGG AACTTGACCA ACAGCAGCAA 
GAATTCCAGC AAAACGTGGA GCGGCTACAG GAGGGTCATA GACTTGTGGA GAGGGAGAAG 
GAGGATCTGG CTGTGAAACT TAAGCTTTGG AGGCACTTTA ACAACCATCA GCCAAGGAGT 
CTATTGACTG AGGGTGTCGG AAGCCGTCGC TCTGTCAGTT TACAAGACGA AGACTCTGTG 
TATATTAACA GTGCCTTGGC TCAGATATCA CTCAACACCA TCACAACATT TCCAGCCCCA 
GACTACCCGA ATGCACATTT GTTTCACAGC CCCAGTTCAC TCCTTGCACA GTCTACTGAG 
AAAAACACAA CGGACACTGC TAATGAGCCT TGGAGCTCTG TGGTCTCTCG CCACCAAAGA 
CTTGAAGGAT CTTCACTCAT TTCCCGGCAC AACAAGGATG CATGTAACAA TGATCTGAAT 
GCAGCTCATG TTATTTCCTG TGGTACGCTC CTGTCTGTGC CTAATGCAAC GCAGCCTGTG 
GAACCACAGA CCAAACCCGC AGAAGAAACC TCACATCATT TGGAAAATGG CAAAATTGAA 
GAGAACATAG TTTACCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_012952887.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808341.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.

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CloneID	OXa35295
Clone ID Related Accession (Same CDS sequence)	XM_012952887.3 , XM_012952887.2
Accession Version	XM_012952887.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952887.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG 
GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC 
AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG 
AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT 
GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC 
TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG 
CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA 
TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG 
GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC 
CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT 
GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC 
ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT 
GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA 
AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA 
CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG 
GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG 
GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA 
AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA 
GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA 
CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG 
AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG 
CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA 
GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG 
GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT 
GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG 
AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC 
TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT 
CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG 
ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT 
GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG 
CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA 
GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG 
CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA 
GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA 
GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT 
GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT 
GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA 
TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT 
ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG 
CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG 
CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT 
TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT 
GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG 
CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA 
AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT 
CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG 
GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA 
CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC 
GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG 
GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT 
TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT 
GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC 
ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC 
TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC 
GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808341.1
CDS	1081..6003
Translation	MMELSLSEVPLYGQMIVFATFGQDLHLPEDAEFYFVYNGSSQRHVIFAERLASNVLRSVFPGHNCPESVIVTVCMHTEGYSPVIVACTPVTYIRDTACNISHYLKMHCGSLSPTSHETILTHFGLTVKDLLHMDRNMMLCLASEESKPIWNILGSASESGSFRGETLLHLTARWGLEEVSHFLMYQPGGVCGLHTENEDQETPIDIAARSGHIGLVARLRNFQDAPPLDFFTVVIGEDSFLRFCHSTTVLTLTRLQPQGKSLESDIELFRKCLWDSRFIEKITNPMQNQDHKILNQPDEIHREEDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEEQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_012952887.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952887.2

ORF Insert Sequence:

ATGATGGAAC TCAGCCTATC CGAAGTGCCT CTCTATGGTC AGATGATTGT ATTTGCAACT 
TTTGGGCAAG ATCTTCATCT GCCGGAAGAT GCAGAATTCT ACTTTGTTTA CAATGGATCC 
AGCCAGAGGC ACGTAATATT TGCAGAGCGC CTCGCTTCAA ATGTCCTGCG CTCTGTTTTT 
CCAGGCCACA ATTGCCCAGA ATCTGTAATT GTTACTGTGT GTATGCACAC CGAGGGCTAC 
TCACCTGTGA TCGTGGCTTG CACGCCTGTA ACATACATAA GGGACACAGC ATGCAATATC 
TCACACTACC TGAAAATGCA CTGTGGGAGT CTATCCCCCA CCAGCCATGA GACAATACTC 
ACTCATTTTG GACTGACTGT CAAGGATTTG CTCCATATGG ACAGAAACAT GATGCTGTGC 
CTCGCTAGTG AAGAATCAAA GCCTATCTGG AATATCCTTG GGTCGGCTTC TGAATCAGGC 
AGCTTTCGTG GCGAGACTTT ACTTCACCTG ACAGCAAGAT GGGGATTGGA AGAAGTCTCT 
CATTTCCTTA TGTACCAGCC TGGTGGAGTG TGTGGATTAC ATACAGAAAA TGAAGATCAG 
GAGACCCCAA TCGATATTGC AGCTCGCAGT GGACACATCG GATTGGTTGC ACGCTTACGA 
AATTTTCAGG ATGCGCCACC CCTTGACTTT TTCACAGTAG TCATCGGAGA GGATTCCTTC 
TTACGCTTCT GTCATTCAAC CACTGTGCTG ACACTGACTC GGCTTCAGCC TCAGGGGAAG 
TCGCTGGAGT CTGACATTGA GCTCTTCAGG AAATGTTTGT GGGACTCCCG CTTCATTGAA 
AAGATAACAA ACCCAATGCA AAATCAGGAT CACAAAATAC TGAACCAGCC CGATGAAATA 
CATAGAGAAG AAGATAAAAG GTATTCATCA ATAGATGTGC TCAAGAAGTT AAAACACCCG 
CCTACATTTC TTGCTGCATC CAGACTTTCT GCCATGCTGA ATGGAAATGA TGAGGTGTAT 
GCCAACAGTA TGGTGGTGGA TGAGGTGACT GATTCAGAGA TCAACTACAT TCATGTGGAC 
AATGCCAATA CTGAAGGCTC ACCTACACAC CGTTCAGACT CCACCCTGAC AGATAAAGCA 
AATAATTATA GTGTTTGTGC ATCTCCTGAG AGAAACAGAC CAGAAGTAAA CAATGTGGAT 
CAGGCAGACC TGCCTGCTTT CCTTTCACCT TCCCACCTGT ACTCAGGTGC AGCACGGAGA 
CAAAGCAGAA CCCTGGGCTA TGCTACTGAA AGGAACACAG CTTGTAATGT CATAAAGAGA 
AGAAGCTCAA GTCTTGATGG TCTCGATGCT GACAGCGAAG GAGAAGGAAC ATATCCAAGA 
TATAGTTCTG GGTCATCCAT TAAACAGGAC TCCCCTGGTC TTCTGGGAAA CAGTGGGGAT 
GAACTTGACT CTCCTGAAAG AAATGCTGAG CCTGACTTTC CAATTAGAGG AGTGGGAACA 
TTGCCTCTGC AGGGAAAGGA TCCACTGGGC ACTGGTGTTC GATTGCGTTC ATACTCGTGT 
TCCTCGCCAA AGCTTTCTGT TGGAATACCT CGCATAGCAA GGGACCTCTC CGTCAGTGAC 
ACAAGTGAGG AACAGCGGGC GTATAGCCTC CCTGATCAAC TGAGAGAAAA AAGGATTGAG 
GAAGAGGAAT GGGATAAATA CGCAACTCCT ACAAAACCAG AATCTGAAAA GAACAAAGTC 
AGCCGGACAT TTAGCTTCTT GCGGAACAGG ATGACCAGCA CCAGGAACAA AAACAAGGTG 
AAAAATAAGG ATATGAAAGA GAAGGTGAAC AGGCATCAGT TTGTGACGGG CACGTTCTCT 
GGGGTGGTTC CATGTTTGGT GTGTGAGAAG GCACTCCTGG GGAAGGAGTC TCTGCAGTGC 
TCCAGTTGTA ATGTGAATGT ACACAAAGGC TGCAAGGAAT CTGCTCCCCA TTGCACCAAG 
CGATTTCAGG AACGATATCA CCCAAAGAAC AAGCAGTCGG CAGTTATATC AAATTCATCA 
TATAAAGATA TCCCACAACA AGTTCAACCT ACAATGCATA CTTCTACATC TGTGCCTCTG 
GGTCTCTCCT CGGGCAAGAA AGAGACCATT CACTCCACTC CCCTGCATTC TAAAACCATC 
CCTGTGTTAA GCTCTGATAG AAGGTCTGAC CCCTCTCTGG AATCAGATGG GGACACCAGT 
GGCTGCAGAT CGAGATCTCA GTCGGAAGAG CTGCTGCAAA CCTTGGGCAC ACCTCCATCC 
ATGGACTGCT TCCCAATTGA AGATGTAGTA GATACAACGT TATGGAGTGA CTTCAGTAAT 
GACATGCAGG AATTCGAAGC CGAGTCATGG AGTCTTGTGG TTGATCCATT ATTCTGTGAA 
AAGCAAAAGA AAGATGTTAT AAAGAGACAA GATGTCATTT TTGAACTGAT CCAGACAGAA 
CTCCATCACG TCCAGACATT GTTGATTATG TCTGAGATAT TCAGAAAAGG AATGAAAGAG 
GAGCTTCAGT TGGACCACAG CACAGTGGAT AAAATATTCC CTTGTCTGGA TGAGCTGCTG 
GAGATCCACA AGCAGTTCTT CTCCAACCTT AAAGAAAGGA AACAGGAATC TAGGGAAGGA 
AACGACAAAA ACTTTATCAT CAACAGAATT GGAGATATCC TCGTGCAACA GTTTTCAGCA 
GACAGTGCCA ATAAAATGAA GAAGATCTAT GGAGAGTTTT GCAGTCACCA TATCGAAGCA 
CTGAACTTGT TTAAGGAGCT ACAGCAGAAC AAGAAATTCC AGAACTTTAT TAAACTAAGG 
AACCGTAATC TCCTGGCACG CCGACGGGGT ATCCCGGAAT GCATCCTTCT TGTAACTCAG 
CGCATCACAA AGTATCCAGT GCTTGTGGAA AGGATATTAA AATACACCAC CGAAGGGACA 
GAGGAGCACA CAGATCTCTG CAAAGCCCTG GTGTTAATGA AGGAGCTGCT GGCAGCAGTG 
GATTTGCAGG TCAATAAGTT TGAGAAAGGA CAAAAGCTGC TAGAGATTCT GAATAAAATT 
GAAAATAAAA CTTGCACCAA ATTAAAGAAT GGCCTAGAAT TCAGAAAGCA GGACCTGACG 
AGCAAAGAGC GGGCGCTGCT GCATGAGGGA GTGGTGTTTT GGAAAACGGC TACCGGGCGC 
TTTAAAGATA TTCAAGCACT TCTGTTAACG GACATCCTGC TGTTTCTACA AGAGAAGGAT 
CAAAAGTATA TTTTTGCTGC AGTGGACCAG AAACCCCCAG TTATTTGCCT TCGGAAACTG 
ATTGTGCGAG AAGTAGCGAA TGAGGAGCGC GGGATGTTTT TAATAAGTGC CTCATCCGCT 
GGGCCTGAAA TGTATGAGAT CCACACCAAT TCCAAGGAAG AGCGCAATGG CTGGATGAGG 
CAGATTCAGG AGGCTGTACA GAGCTGTCCG CAGGAAGATG AAGGTAAAAG CTGTGAATCA 
GATGAAGATA AACGGGTGGC AGAGGCCAGA GCAGTGAAGG CTCAAAAGTA TCATGATACG 
CTGACCCTCC AAGATCAGAA TATCTGTAAT ACTTTAGAGG AAAAATTACA GATCTACGCA 
GAACTTGCAG CCTTAAACAC AAAGAGTGAC ATGCACTTGG AACCTCACCT TTTTATCAAA 
GCCGATTCAG GGGAAATCCC TCAGGCGGCG GCTCTGCTGG CTGCTGCACT CAAGGAAGCT 
GAAAATCTCC ACTCAACCAT TAAATCACAA ATGGAGGATC CATCTTGGTT ACCTGAATAT 
GAAAGAAGCA CTCCGAGAAG ATGTCGTTCT GTTGAAACAT TAGATGAGGA ACCGGAGACA 
TCATATAACT CCCAGAGCCC AGATGCAAGG GAGAGAAGGA AGCTTTCCTA TGATGCCAGT 
ATTTCTCATG TGAAGAGTGA CAGGGATCTG GATTTCACAG AAGAAATTGA GGAATGTATG 
CAAAGTCCAA GCTATAATCA GGATGAAGTT CTACAAGTGG TGCATAACCT TACTCGCCTG 
CTGTACAGCA TACAGGCTAC TGTTGCTATG CAAGATAGCC ACATAGAACT GCATAAAGCT 
TTCCTACAGG ATTATGAATT GGGCAGTAGA GGTTCCCGTG GAAGTATACT TAATGAACAT 
GAGAAGTATC GCAACCTAGA AAAGCACCGC TGCGAGATGG TCAATTTACA TAAACTCCAG 
CAACAGTTGC ATCAAGAACA ACAACGCTGG CTGCGCGAGT GCGACCAAAA ACAGAGGGAA 
AACGAGGAGA AGGAGAGCAT GCTCCAGCAT AGGGAGCAAG AGTGTCAGAG TCAGGAACAT 
CTCTTGCACC AAAAGAGGGA GGAACTTGAC CAACAGCAGC AAGAATTCCA GCAAAACGTG 
GAGCGGCTAC AGGAGGGTCA TAGACTTGTG GAGAGGGAGA AGGAGGATCT GGCTGTGAAA 
CTTAAGCTTT GGAGGCACTT TAACAACCAT CAGCCAAGGA GTCTATTGAC TGAGGGTGTC 
GGAAGCCGTC GCTCTGTCAG TTTACAAGAC GAAGACTCTG TGTATATTAA CAGTGCCTTG 
GCTCAGATAT CACTCAACAC CATCACAACA TTTCCAGCCC CAGACTACCC GAATGCACAT 
TTGTTTCACA GCCCCAGTTC ACTCCTTGCA CAGTCTACTG AGAAAAACAC AACGGACACT 
GCTAATGAGC CTTGGAGCTC TGTGGTCTCT CGCCACCAAA GACTTGAAGG ATCTTCACTC 
ATTTCCCGGC ACAACAAGGA TGCATGTAAC AATGATCTGA ATGCAGCTCA TGTTATTTCC 
TGTGGTACGC TCCTGTCTGT GCCTAATGCA ACGCAGCCTG TGGAACCACA GACCAAACCC 
GCAGAAGAAA CCTCACATCA TTTGGAAAAT GGCAAAATTG AAGAGAACAT AGTTTACCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef28 ( XM_012952889.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012808343.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.

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CloneID	OXa35296
Clone ID Related Accession (Same CDS sequence)	XM_012952889.2 , XM_012952889.3
Accession Version	XM_012952889.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 28 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG 
TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT 
GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC 
ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG 
ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA 
AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT 
GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT 
GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA 
ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA 
AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA 
GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT 
TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC 
TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA 
GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC 
CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT 
CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC 
AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG 
AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG 
AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA 
GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG 
AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA 
CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC 
ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT 
GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA 
GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA 
GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA 
TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA 
CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT 
ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG 
GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC 
CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA 
ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC 
TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG 
AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG 
GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG 
GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC 
CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA 
GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG 
AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG 
GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA 
ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC 
CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG 
CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC 
AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT 
CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC 
AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT 
AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT 
GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG 
GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA 
CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT 
TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA 
AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT 
CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA 
GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT 
ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT 
AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC 
CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC 
TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG 
CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT 
GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT 
GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC 
CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA 
GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA 
ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT 
GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC 
TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT 
AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT 
GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA 
AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012808343.1
CDS	289..4464
Translation	MESDSDSPFNYSWPSFPKMRIHRRSSKQDKRYSSIDVLKKLKHPPTFLAASRLSAMLNGNDEVYANSMVVDEVTDSEINYIHVDNANTEGSPTHRSDSTLTDKANNYSVCASPERNRPEVNNVDQADLPAFLSPSHLYSGAARRQSRTLGYATERNTACNVIKRRSSSLDGLDADSEGEGTYPRYSSGSSIKQDSPGLLGNSGDELDSPERNAEPDFPIRGVGTLPLQGKDPLGTGVRLRSYSCSSPKLSVGIPRIARDLSVSDTSEDGVVSFSGRSLLQALSLSKSVYLLHPGKQRAYSLPDQLREKRIEEEEWDKYATPTKPESEKNKVSRTFSFLRNRMTSTRNKNKVKNKDMKEKVNRHQFVTGTFSGVVPCLVCEKALLGKESLQCSSCNVNVHKGCKESAPHCTKRFQERYHPKNKQSAVISNSSYKDIPQQVQPTMHTSTSVPLGLSSGKKETIHSTPLHSKTIPVLSSDRRSDPSLESDGDTSGCRSRSQSEELLQTLGTPPSMDCFPIEDVVDTTLWSDFSNDMQEFEAESWSLVVDPLFCEKQKKDVIKRQDVIFELIQTELHHVQTLLIMSEIFRKGMKEELQLDHSTVDKIFPCLDELLEIHKQFFSNLKERKQESREGNDKNFIINRIGDILVQQFSADSANKMKKIYGEFCSHHIEALNLFKELQQNKKFQNFIKLRNRNLLARRRGIPECILLVTQRITKYPVLVERILKYTTEGTEEHTDLCKALVLMKELLAAVDLQVNKFEKGQKLLEILNKIENKTCTKLKNGLEFRKQDLTSKERALLHEGVVFWKTATGRFKDIQALLLTDILLFLQEKDQKYIFAAVDQKPPVICLRKLIVREVANEERGMFLISASSAGPEMYEIHTNSKEERNGWMRQIQEAVQSCPQEDEGKSCESDEDKRVAEARAVKAQKYHDTLTLQDQNICNTLEEKLQIYAELAALNTKSDMHLEPHLFIKADSGEIPQAAALLAAALKEAENLHSTIKSQMEDPSWLPEYERSTPRRCRSVETLDEEPETSYNSQSPDARERRKLSYDASISHVKSDRDLDFTEEIEECMQSPSYNQDEVLQVVHNLTRLLYSIQATVAMQDSHIELHKAFLQDYELGSRGSRGSILNEHEKYRNLEKHRCEMVNLHKLQQQLHQEQQRWLRECDQKQRENEEKESMLQHREQECQSQEHLLHQKREELDQQQQEFQQNVERLQEGHRLVEREKEDLAVKLKLWRHFNNHQPRSLLTEGVGSRRSVSLQDEDSVYINSALAQISLNTITTFPAPDYPNAHLFHSPSSLLAQSTEKNTTDTANEPWSSVVSRHQRLEGSSLISRHNKDACNNDLNAAHVISCGTLLSVPNATQPVEPQTKPAEETSHHLENGKIEENIVYL

Target ORF information:

RefSeq Version	XM_012952889.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 28 (arhgef28), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012952889.2

ORF Insert Sequence:

ATGGAATCAG ACTCAGACTC ACCTTTCAAT TATTCCTGGC CTTCATTTCC AAAAATGAGG 
ATTCACAGAA GGTCATCTAA ACAAGATAAA AGGTATTCAT CAATAGATGT GCTCAAGAAG 
TTAAAACACC CGCCTACATT TCTTGCTGCA TCCAGACTTT CTGCCATGCT GAATGGAAAT 
GATGAGGTGT ATGCCAACAG TATGGTGGTG GATGAGGTGA CTGATTCAGA GATCAACTAC 
ATTCATGTGG ACAATGCCAA TACTGAAGGC TCACCTACAC ACCGTTCAGA CTCCACCCTG 
ACAGATAAAG CAAATAATTA TAGTGTTTGT GCATCTCCTG AGAGAAACAG ACCAGAAGTA 
AACAATGTGG ATCAGGCAGA CCTGCCTGCT TTCCTTTCAC CTTCCCACCT GTACTCAGGT 
GCAGCACGGA GACAAAGCAG AACCCTGGGC TATGCTACTG AAAGGAACAC AGCTTGTAAT 
GTCATAAAGA GAAGAAGCTC AAGTCTTGAT GGTCTCGATG CTGACAGCGA AGGAGAAGGA 
ACATATCCAA GATATAGTTC TGGGTCATCC ATTAAACAGG ACTCCCCTGG TCTTCTGGGA 
AACAGTGGGG ATGAACTTGA CTCTCCTGAA AGAAATGCTG AGCCTGACTT TCCAATTAGA 
GGAGTGGGAA CATTGCCTCT GCAGGGAAAG GATCCACTGG GCACTGGTGT TCGATTGCGT 
TCATACTCGT GTTCCTCGCC AAAGCTTTCT GTTGGAATAC CTCGCATAGC AAGGGACCTC 
TCCGTCAGTG ACACAAGTGA GGATGGTGTG GTCAGCTTCA GTGGTCGCTC ACTCCTCCAA 
GCACTTTCAT TGTCTAAATC AGTATATCTT CTTCACCCTG GTAAACAGCG GGCGTATAGC 
CTCCCTGATC AACTGAGAGA AAAAAGGATT GAGGAAGAGG AATGGGATAA ATACGCAACT 
CCTACAAAAC CAGAATCTGA AAAGAACAAA GTCAGCCGGA CATTTAGCTT CTTGCGGAAC 
AGGATGACCA GCACCAGGAA CAAAAACAAG GTGAAAAATA AGGATATGAA AGAGAAGGTG 
AACAGGCATC AGTTTGTGAC GGGCACGTTC TCTGGGGTGG TTCCATGTTT GGTGTGTGAG 
AAGGCACTCC TGGGGAAGGA GTCTCTGCAG TGCTCCAGTT GTAATGTGAA TGTACACAAA 
GGCTGCAAGG AATCTGCTCC CCATTGCACC AAGCGATTTC AGGAACGATA TCACCCAAAG 
AACAAGCAGT CGGCAGTTAT ATCAAATTCA TCATATAAAG ATATCCCACA ACAAGTTCAA 
CCTACAATGC ATACTTCTAC ATCTGTGCCT CTGGGTCTCT CCTCGGGCAA GAAAGAGACC 
ATTCACTCCA CTCCCCTGCA TTCTAAAACC ATCCCTGTGT TAAGCTCTGA TAGAAGGTCT 
GACCCCTCTC TGGAATCAGA TGGGGACACC AGTGGCTGCA GATCGAGATC TCAGTCGGAA 
GAGCTGCTGC AAACCTTGGG CACACCTCCA TCCATGGACT GCTTCCCAAT TGAAGATGTA 
GTAGATACAA CGTTATGGAG TGACTTCAGT AATGACATGC AGGAATTCGA AGCCGAGTCA 
TGGAGTCTTG TGGTTGATCC ATTATTCTGT GAAAAGCAAA AGAAAGATGT TATAAAGAGA 
CAAGATGTCA TTTTTGAACT GATCCAGACA GAACTCCATC ACGTCCAGAC ATTGTTGATT 
ATGTCTGAGA TATTCAGAAA AGGAATGAAA GAGGAGCTTC AGTTGGACCA CAGCACAGTG 
GATAAAATAT TCCCTTGTCT GGATGAGCTG CTGGAGATCC ACAAGCAGTT CTTCTCCAAC 
CTTAAAGAAA GGAAACAGGA ATCTAGGGAA GGAAACGACA AAAACTTTAT CATCAACAGA 
ATTGGAGATA TCCTCGTGCA ACAGTTTTCA GCAGACAGTG CCAATAAAAT GAAGAAGATC 
TATGGAGAGT TTTGCAGTCA CCATATCGAA GCACTGAACT TGTTTAAGGA GCTACAGCAG 
AACAAGAAAT TCCAGAACTT TATTAAACTA AGGAACCGTA ATCTCCTGGC ACGCCGACGG 
GGTATCCCGG AATGCATCCT TCTTGTAACT CAGCGCATCA CAAAGTATCC AGTGCTTGTG 
GAAAGGATAT TAAAATACAC CACCGAAGGG ACAGAGGAGC ACACAGATCT CTGCAAAGCC 
CTGGTGTTAA TGAAGGAGCT GCTGGCAGCA GTGGATTTGC AGGTCAATAA GTTTGAGAAA 
GGACAAAAGC TGCTAGAGAT TCTGAATAAA ATTGAAAATA AAACTTGCAC CAAATTAAAG 
AATGGCCTAG AATTCAGAAA GCAGGACCTG ACGAGCAAAG AGCGGGCGCT GCTGCATGAG 
GGAGTGGTGT TTTGGAAAAC GGCTACCGGG CGCTTTAAAG ATATTCAAGC ACTTCTGTTA 
ACGGACATCC TGCTGTTTCT ACAAGAGAAG GATCAAAAGT ATATTTTTGC TGCAGTGGAC 
CAGAAACCCC CAGTTATTTG CCTTCGGAAA CTGATTGTGC GAGAAGTAGC GAATGAGGAG 
CGCGGGATGT TTTTAATAAG TGCCTCATCC GCTGGGCCTG AAATGTATGA GATCCACACC 
AATTCCAAGG AAGAGCGCAA TGGCTGGATG AGGCAGATTC AGGAGGCTGT ACAGAGCTGT 
CCGCAGGAAG ATGAAGGTAA AAGCTGTGAA TCAGATGAAG ATAAACGGGT GGCAGAGGCC 
AGAGCAGTGA AGGCTCAAAA GTATCATGAT ACGCTGACCC TCCAAGATCA GAATATCTGT 
AATACTTTAG AGGAAAAATT ACAGATCTAC GCAGAACTTG CAGCCTTAAA CACAAAGAGT 
GACATGCACT TGGAACCTCA CCTTTTTATC AAAGCCGATT CAGGGGAAAT CCCTCAGGCG 
GCGGCTCTGC TGGCTGCTGC ACTCAAGGAA GCTGAAAATC TCCACTCAAC CATTAAATCA 
CAAATGGAGG ATCCATCTTG GTTACCTGAA TATGAAAGAA GCACTCCGAG AAGATGTCGT 
TCTGTTGAAA CATTAGATGA GGAACCGGAG ACATCATATA ACTCCCAGAG CCCAGATGCA 
AGGGAGAGAA GGAAGCTTTC CTATGATGCC AGTATTTCTC ATGTGAAGAG TGACAGGGAT 
CTGGATTTCA CAGAAGAAAT TGAGGAATGT ATGCAAAGTC CAAGCTATAA TCAGGATGAA 
GTTCTACAAG TGGTGCATAA CCTTACTCGC CTGCTGTACA GCATACAGGC TACTGTTGCT 
ATGCAAGATA GCCACATAGA ACTGCATAAA GCTTTCCTAC AGGATTATGA ATTGGGCAGT 
AGAGGTTCCC GTGGAAGTAT ACTTAATGAA CATGAGAAGT ATCGCAACCT AGAAAAGCAC 
CGCTGCGAGA TGGTCAATTT ACATAAACTC CAGCAACAGT TGCATCAAGA ACAACAACGC 
TGGCTGCGCG AGTGCGACCA AAAACAGAGG GAAAACGAGG AGAAGGAGAG CATGCTCCAG 
CATAGGGAGC AAGAGTGTCA GAGTCAGGAA CATCTCTTGC ACCAAAAGAG GGAGGAACTT 
GACCAACAGC AGCAAGAATT CCAGCAAAAC GTGGAGCGGC TACAGGAGGG TCATAGACTT 
GTGGAGAGGG AGAAGGAGGA TCTGGCTGTG AAACTTAAGC TTTGGAGGCA CTTTAACAAC 
CATCAGCCAA GGAGTCTATT GACTGAGGGT GTCGGAAGCC GTCGCTCTGT CAGTTTACAA 
GACGAAGACT CTGTGTATAT TAACAGTGCC TTGGCTCAGA TATCACTCAA CACCATCACA 
ACATTTCCAG CCCCAGACTA CCCGAATGCA CATTTGTTTC ACAGCCCCAG TTCACTCCTT 
GCACAGTCTA CTGAGAAAAA CACAACGGAC ACTGCTAATG AGCCTTGGAG CTCTGTGGTC 
TCTCGCCACC AAAGACTTGA AGGATCTTCA CTCATTTCCC GGCACAACAA GGATGCATGT 
AACAATGATC TGAATGCAGC TCATGTTATT TCCTGTGGTA CGCTCCTGTC TGTGCCTAAT 
GCAACGCAGC CTGTGGAACC ACAGACCAAA CCCGCAGAAG AAACCTCACA TCATTTGGAA 
AATGGCAAAA TTGAAGAGAA CATAGTTTAC CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	arhgef28
Entrez Gene ID	100492169
Full Name	Rho guanine nucleotide exchange factor 28
General protein information	Preferred Names Rho guanine nucleotide exchange factor 28 Names rho guanine nucleotide exchange factor 28 Rho guanine nucleotide exchange factor (GEF) 28
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	ARHGEF28	XP_001151239.2
Rattus norvegicus (Norway rat)	Arhgef28	NP_001102012.1
Gallus gallus (chicken)	LOC427201	XP_424789.4
Danio rerio (zebrafish)	arhgef28	XP_688428.4
Homo sapiens (human)	ARHGEF28	NP_001073948.2
Bos taurus (cattle)	RGNEF	NP_001095777.2
	ARHGEF28	XP_001101425.1
Canis lupus familiaris (dog)	ARHGEF28	XP_005617529.1
Mus musculus (house mouse)	Arhgef28	NP_036156.2
Xenopus tropicalis (tropical clawed frog)	arhgef28	XP_002934885.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

arhgef28 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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