MCF2L2 cDNA ORF clone, Gallus gallus(chicken)

The following MCF2L2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCF2L2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa152684 XM_025153435.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa152685 XM_025153436.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa152686 XM_025153438.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OGa39983 XM_015291524.2
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa12709 XM_004943437.3
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12710 XM_004943438.3
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12711 XM_004943439.3
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12713 XM_004943441.3
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12714 XM_025153437.1
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12714 XM_004943442.3
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12715 XM_004943443.3
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12716 XM_422768.6
Latest version!
Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OGa12713 XM_004943441.2 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12709 XM_004943437.2 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12716 XM_422768.5 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12711 XM_004943439.2 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa39983 XM_015291524.1 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OGa12714 XM_004943442.2 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12710 XM_004943438.2 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa12715 XM_004943443.2 Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa152684
    Clone ID Related Accession (Same CDS sequence) XM_025153435.1
    Accession Version XM_025153435.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATCCAAT ACATTGCACT ACTATGTCAG CAAACAGGAG ATGAAATCAT GCAGCAGGAG 
    ATACGACCGC TGGTTGCAGT GGATATAATA GAACAGCTAC ACAGGCAATT TGCTATCTTG
    TCAGGAGGAA GAGGGAAGGA TGGAGCGCCA ATTATTACCT TCCCAGAGTT TGCAGGATTC
    AGTGAAATAC CCGATGAGGA TTTTCTGAAT GTGGTCACGT ACCTAACCAG CATTCCCAGT
    CTGGAGGCTG CCAGCATTGG ATTCATCATC ATCATAGACA GACGACGAGA CAAATGGAGC
    GCAGTCAAGG CATCCCTGAC ACGGATAGCA GGAGCATTTC CAGGAAACCT ACAGCTGGTG
    TTTGTCCTGC GTCCCTCACG CTTTATCCAA AGGGCAATCG CTGACATTGG CATTAAACTC
    TATCGAGATG ACTTTAAAAT GAAGGTACCG ATCATCATGC TAAATTCTGT CTCTGATCTT
    CATGGCTATA TTGATAAATG TCAACTGACC CGGGAGCTGG GAGGAACCCT GGAGTACGGC
    CACAGTCAGT GGATACATCA CCGAACTGCT ATTGAAAACT TTGCAATGAC AGTGAAAACA
    ACAGCACAGA TGCTACAGAC CTTTGGAACG GACTTAGCAG AGACTGAACT TCCCAATGAT
    GTGCAGTGTA CAGAGGAGCT CCTATCTGCA CACACTGACC ACCATAGCAA GCTGAAGGAT
    GAGCTGAAAC TAGCTGTGAA GCAGGGGGCC ACACTACTGA CGTGCATTAG GGAGCCAGTC
    ACCCGAAGTG CCAACAGCAA GCTCAGTCCA GATGAACTGG AAAACGTGGC AACAGTGGAA
    AGGTTGTTGG CTCAGTTGGA TGAAACAGAA AAGGCTTTTG ATCAATTTTG GACCAAGCAT
    CACCTAAAAC TAGAACAATG CTTGCAGCTT CGACACTTTG AACATGATTT TAGAGAAGTA
    AAATTAACAT TGGATAATCT CATGGAAGCA CAAGCTGGTT TTTCTGACGT TGGAGACAGT
    GTGACTCGAG TAGAACACCT CCTAAGGGAA CAGAAGCAAC TGGAAGAAAA AGGGCAGGAA
    CCTTTGGAGA AAGCCCAGAC TCTAGCTCTC CATGGAGAAC AGCTCATCCA AAACAACCAT
    TATGCAGTTG ACTCCATTAG ACCAAAGTGT GTTGAACTCA GGCGTATTTG CGATGACTTT
    ACTAATGAGA CCAAGAAAAA GTATGATATT TTGGGGAAGT CTCTTGAGCT GCATAAGCAA
    TTAGATAAGG CAAGCCAATG GTGTGAAGCT GGAATCTACC TCTTAGCTTC CCAAGCTGTG
    GACAAGTGCC AGTCACAAGA GGGAGCTGAA ACTGCACTCG TTGAAATAGA GAAGTTCCTG
    GCAACAGCAA AGGAGCACCA ACTCTCTAAC CCAAAGGAGT TCTACAATCA GTTTGACACG
    ATTCTCACAC CTGAAATAAA GGCCAACGCC CAAAGGATTT TACAAAAGCT AGAAGATGTA
    CAAGAGATGT TTGACAAGAG GCAAGTGAGT CTGAAGAAGC TAGCAGCCAA ACAGACCCGT
    CCAGTGCAAC CTGTTGCCCC ACATCCTGAA TCTTCCCCAA AACGAGCATC ACCAAAGACT
    ACCAGACCAG CAGGAGTAGC TACCAACAGG AGGTCCGCAG AAATTACTTG TCCTCCAAAG
    TCCATCTCTG AGGCAGAGTT ATCAAAAAAG AAAAGCATTA AGAAGACTAA AGGTGGAATT
    AAGATTGAAG TGATGCATGA AGCGAGCCAA GGGGGATCTA GCAGAGTCTT GGTTATGAGT
    GAAAGCGATG AGAACCTGTC GACGAGGAGG AGCCACATAA TCAATGAGCT GATAGAAACC
    GAACGCGTTT ATGTGGAAGA ACTTCAAAGC ATAATAGAGG GATATGCTTC AGAAATGGAC
    AATCCAAGTT TATTACATCT GATTCCATCA GTGCTCCAGA ACAAAAAGGA TATTCTATTT
    GGGAACCTCC CAGAAATCTA TGAATTCCAC AACAGGATTT TCCTTAAAGA GTTAGAAAAT
    TGCATTGAAA ATCCTGAGCT CCTTGCCCGA TGCTTTTTGA AAAGGAAAGA GGACCTTCAA
    ATATATGAAA AATACTGTCA GAACAAGCCA AGGTCTGAAG CTCTCTGGAG ACAGTGTGGA
    GACAGCATCT TTTTTCAGGA ATGTCAGCGT AAATTGGATC ATAAGCTCTC ACTAGATGCG
    TATCTTTTAA AGCCAGTCCA GAGAATCACC AAGTACCAGC TACTATTGAA GGAAATGCTG
    AAATGTAGCA AGAATTCAGA AGGTACTGCT GAACTGGAAG AGGCCTTGGC CACAGTGCTT
    GATATCATCA AATCCGTAAA TGACTCCATG CACCAGATAG CAATCACAGG ATATGAGGGG
    GACGTAAGTG AGCTTGGTAA GCTGTTGATG CAAGGTTCCT TCAACGTGTG GACTGACCAC
    AAGAAGGGTC ACAACAAGGT GAAGGACTTG GCAAGATTCA AGCCGATGCA GAGACACCTC
    TTCCTCTACA CAAAGATGCT GCTTTTTTGC AAGAAACGAG AGGAAAACAC AGATGGTCAT
    GAGAAGACAG CTTCATACAG CTTTAAAAAT TCATTAAAGA TGAGCACAGT GGGCATCACA
    GAAAATGTAA AAGGAGACAA CAAGAAGTTT GAGATCTGGT ATAATGGCAG AGAAGAAGTC
    TATATCATTC AGGCATCTTC TGTTGAACTG AAAAACACTT GGATTTCCGA GATACGCAAA
    GTTCTAACAG GACAACTGGA AGCATGTAGA GAAGCAAGTC AACTACACCA AAGAATCACC
    GAGAGTGTGT ACCATGCACC AATGAATTCC TCCAATATAA CCAGGTATAG CAGAAAGTCG
    TCAAGCATAT ATGAAAACAA ATCCGAGACA TCAAACGTGG AAGCATCTAA CAATAAAAAC
    AGGAATTCCA GTCCCAGTGC CAGACAAAAG AGAGTTGATG CTTCCACCAG CCTTAACCTT
    GAGCGCACAG CGCTTGCTAG AAAGCGATTC ACATTGCAAG GTCTTTCCAA CCGCAGAGCT
    CCACCCAAAG ATCCAGAATC TAAGGAAAGA GAAGTTGCCC GTCGCTTTTC CCTAGCCTCC
    TCCATCAGCA CTACAGTTAA TGCATCTGCT GTGACCAAAG GTCCCCTTGT TCCTGCAGTT
    AAAGTCAAGA GACATGAAAT AAAGAGTGAC CCAACTCCCC TGGGGTTTGA AGATGCTTTT
    GAGAACACCA TATTGGCCCA GACTAAATGT AGCACTGGTT CCACCACAAG ACCCGTACCT
    AGATCTGCTT CACAGACAGG TTGGCCCAGT AGGCAAATGC CATCTCTAGA CACATTTGAA
    GACTTTGAAG CCATTCCTTC CAGCATGGAT GAACTCTCCA ACTCTTCAGA CATGGAGGAA
    GAAACCAACC CTAACAATGG GACCAATCTT TTTCGAGTAG AAGGCAGTTT TGACAGCTGT
    TTCCCAGATT CTCTTACACT TGAAGATGGA GATCTAGTGC AGTTTGTGCA GGAAGGAGAA
    AATGGGCAAT GGTTAGTGAA GAAATTGGTC TCGGAGAAAA GTGAATGGGT TCCTTCCAGT
    ATATTGCAGC CAGCAGAGGG GGACACTAAC AACATAATTA AGAACAGCAA TGCAGACATG
    TACAATGATT CCTGCAGCAC GGCTTCAGTT GAGTCGGACA CCAAGGAGAG TGAGGTGGCT
    AAAAGCTTCC CAGCAGAACA GAGGGCTGGC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009203.1
    CDS47..3802
    Translation

    Target ORF information:

    RefSeq Version XM_025153435.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153435.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGATCCAAT ACATTGCACT ACTATGTCAG CAAACAGGAG ATGAAATCAT GCAGCAGGAG 
    ATACGACCGC TGGTTGCAGT GGATATAATA GAACAGCTAC ACAGGCAATT TGCTATCTTG
    TCAGGAGGAA GAGGGAAGGA TGGAGCGCCA ATTATTACCT TCCCAGAGTT TGCAGGATTC
    AGTGAAATAC CCGATGAGGA TTTTCTGAAT GTGGTCACGT ACCTAACCAG CATTCCCAGT
    CTGGAGGCTG CCAGCATTGG ATTCATCATC ATCATAGACA GACGACGAGA CAAATGGAGC
    GCAGTCAAGG CATCCCTGAC ACGGATAGCA GGAGCATTTC CAGGAAACCT ACAGCTGGTG
    TTTGTCCTGC GTCCCTCACG CTTTATCCAA AGGGCAATCG CTGACATTGG CATTAAACTC
    TATCGAGATG ACTTTAAAAT GAAGGTACCG ATCATCATGC TAAATTCTGT CTCTGATCTT
    CATGGCTATA TTGATAAATG TCAACTGACC CGGGAGCTGG GAGGAACCCT GGAGTACGGC
    CACAGTCAGT GGATACATCA CCGAACTGCT ATTGAAAACT TTGCAATGAC AGTGAAAACA
    ACAGCACAGA TGCTACAGAC CTTTGGAACG GACTTAGCAG AGACTGAACT TCCCAATGAT
    GTGCAGTGTA CAGAGGAGCT CCTATCTGCA CACACTGACC ACCATAGCAA GCTGAAGGAT
    GAGCTGAAAC TAGCTGTGAA GCAGGGGGCC ACACTACTGA CGTGCATTAG GGAGCCAGTC
    ACCCGAAGTG CCAACAGCAA GCTCAGTCCA GATGAACTGG AAAACGTGGC AACAGTGGAA
    AGGTTGTTGG CTCAGTTGGA TGAAACAGAA AAGGCTTTTG ATCAATTTTG GACCAAGCAT
    CACCTAAAAC TAGAACAATG CTTGCAGCTT CGACACTTTG AACATGATTT TAGAGAAGTA
    AAATTAACAT TGGATAATCT CATGGAAGCA CAAGCTGGTT TTTCTGACGT TGGAGACAGT
    GTGACTCGAG TAGAACACCT CCTAAGGGAA CAGAAGCAAC TGGAAGAAAA AGGGCAGGAA
    CCTTTGGAGA AAGCCCAGAC TCTAGCTCTC CATGGAGAAC AGCTCATCCA AAACAACCAT
    TATGCAGTTG ACTCCATTAG ACCAAAGTGT GTTGAACTCA GGCGTATTTG CGATGACTTT
    ACTAATGAGA CCAAGAAAAA GTATGATATT TTGGGGAAGT CTCTTGAGCT GCATAAGCAA
    TTAGATAAGG CAAGCCAATG GTGTGAAGCT GGAATCTACC TCTTAGCTTC CCAAGCTGTG
    GACAAGTGCC AGTCACAAGA GGGAGCTGAA ACTGCACTCG TTGAAATAGA GAAGTTCCTG
    GCAACAGCAA AGGAGCACCA ACTCTCTAAC CCAAAGGAGT TCTACAATCA GTTTGACACG
    ATTCTCACAC CTGAAATAAA GGCCAACGCC CAAAGGATTT TACAAAAGCT AGAAGATGTA
    CAAGAGATGT TTGACAAGAG GCAAGTGAGT CTGAAGAAGC TAGCAGCCAA ACAGACCCGT
    CCAGTGCAAC CTGTTGCCCC ACATCCTGAA TCTTCCCCAA AACGAGCATC ACCAAAGACT
    ACCAGACCAG CAGGAGTAGC TACCAACAGG AGGTCCGCAG AAATTACTTG TCCTCCAAAG
    TCCATCTCTG AGGCAGAGTT ATCAAAAAAG AAAAGCATTA AGAAGACTAA AGGTGGAATT
    AAGATTGAAG TGATGCATGA AGCGAGCCAA GGGGGATCTA GCAGAGTCTT GGTTATGAGT
    GAAAGCGATG AGAACCTGTC GACGAGGAGG AGCCACATAA TCAATGAGCT GATAGAAACC
    GAACGCGTTT ATGTGGAAGA ACTTCAAAGC ATAATAGAGG GATATGCTTC AGAAATGGAC
    AATCCAAGTT TATTACATCT GATTCCATCA GTGCTCCAGA ACAAAAAGGA TATTCTATTT
    GGGAACCTCC CAGAAATCTA TGAATTCCAC AACAGGATTT TCCTTAAAGA GTTAGAAAAT
    TGCATTGAAA ATCCTGAGCT CCTTGCCCGA TGCTTTTTGA AAAGGAAAGA GGACCTTCAA
    ATATATGAAA AATACTGTCA GAACAAGCCA AGGTCTGAAG CTCTCTGGAG ACAGTGTGGA
    GACAGCATCT TTTTTCAGGA ATGTCAGCGT AAATTGGATC ATAAGCTCTC ACTAGATGCG
    TATCTTTTAA AGCCAGTCCA GAGAATCACC AAGTACCAGC TACTATTGAA GGAAATGCTG
    AAATGTAGCA AGAATTCAGA AGGTACTGCT GAACTGGAAG AGGCCTTGGC CACAGTGCTT
    GATATCATCA AATCCGTAAA TGACTCCATG CACCAGATAG CAATCACAGG ATATGAGGGG
    GACGTAAGTG AGCTTGGTAA GCTGTTGATG CAAGGTTCCT TCAACGTGTG GACTGACCAC
    AAGAAGGGTC ACAACAAGGT GAAGGACTTG GCAAGATTCA AGCCGATGCA GAGACACCTC
    TTCCTCTACA CAAAGATGCT GCTTTTTTGC AAGAAACGAG AGGAAAACAC AGATGGTCAT
    GAGAAGACAG CTTCATACAG CTTTAAAAAT TCATTAAAGA TGAGCACAGT GGGCATCACA
    GAAAATGTAA AAGGAGACAA CAAGAAGTTT GAGATCTGGT ATAATGGCAG AGAAGAAGTC
    TATATCATTC AGGCATCTTC TGTTGAACTG AAAAACACTT GGATTTCCGA GATACGCAAA
    GTTCTAACAG GACAACTGGA AGCATGTAGA GAAGCAAGTC AACTACACCA AAGAATCACC
    GAGAGTGTGT ACCATGCACC AATGAATTCC TCCAATATAA CCAGGTATAG CAGAAAGTCG
    TCAAGCATAT ATGAAAACAA ATCCGAGACA TCAAACGTGG AAGCATCTAA CAATAAAAAC
    AGGAATTCCA GTCCCAGTGC CAGACAAAAG AGAGTTGATG CTTCCACCAG CCTTAACCTT
    GAGCGCACAG CGCTTGCTAG AAAGCGATTC ACATTGCAAG GTCTTTCCAA CCGCAGAGCT
    CCACCCAAAG ATCCAGAATC TAAGGAAAGA GAAGTTGCCC GTCGCTTTTC CCTAGCCTCC
    TCCATCAGCA CTACAGTTAA TGCATCTGCT GTGACCAAAG GTCCCCTTGT TCCTGCAGTT
    AAAGTCAAGA GACATGAAAT AAAGAGTGAC CCAACTCCCC TGGGGTTTGA AGATGCTTTT
    GAGAACACCA TATTGGCCCA GACTAAATGT AGCACTGGTT CCACCACAAG ACCCGTACCT
    AGATCTGCTT CACAGACAGG TTGGCCCAGT AGGCAAATGC CATCTCTAGA CACATTTGAA
    GACTTTGAAG CCATTCCTTC CAGCATGGAT GAACTCTCCA ACTCTTCAGA CATGGAGGAA
    GAAACCAACC CTAACAATGG GACCAATCTT TTTCGAGTAG AAGGCAGTTT TGACAGCTGT
    TTCCCAGATT CTCTTACACT TGAAGATGGA GATCTAGTGC AGTTTGTGCA GGAAGGAGAA
    AATGGGCAAT GGTTAGTGAA GAAATTGGTC TCGGAGAAAA GTGAATGGGT TCCTTCCAGT
    ATATTGCAGC CAGCAGAGGG GGACACTAAC AACATAATTA AGAACAGCAA TGCAGACATG
    TACAATGATT CCTGCAGCAC GGCTTCAGTT GAGTCGGACA CCAAGGAGAG TGAGGTGGCT
    AAAAGCTTCC CAGCAGAACA GAGGGCTGGC AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152685
    Clone ID Related Accession (Same CDS sequence) XM_025153436.1
    Accession Version XM_025153436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3744bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGATCC AGAATCTAAG GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA
    GCCTCCTCCA TCAGCACTAC AGTTAATGCA TCTGCTGTGA CCAAAGTTAA AGTCAAGAGA
    CATGAAATAA AGAGTGACCC AACTCCCCTG GGGTTTGAAG ATGCTTTTGA GAACACCATA
    TTGGCCCAGA CTAAATGTAG CACTGGTTCC ACCACAAGAC CCGTACCTAG ATCTGCTTCA
    CAGACAGGTT GGCCCAGTAG GCAAATGCCA TCTCTAGACA CATTTGAAGA CTTTGAAGCC
    ATTCCTTCCA GCATGGATGA ACTCTCCAAC TCTTCAGACA TGGAGGAAGA AACCAACCCT
    AACAATGGGA CCAATCTTTT TCGAGTAGAA GGCAGTTTTG ACAGCTGTTT CCCAGATTCT
    CTTACACTTG AAGATGGAGA TCTAGTGCAG TTTGTGCAGG AAGGAGAAAA TGGGCAATGG
    TTAGTGAAGA AATTGGTCTC GGAGAAAAGT GAATGGGTTC CTTCCAGTAT ATTGCAGCCA
    GCAGAGGGGG ACACTAACAA CATAATTAAG AACAGCAATG CAGACATGTA CAATGATTCC
    TGCAGCACGG CTTCAGTTGA GTCGGACACC AAGGAGAGTG AGGTGGCTAA AAGCTTCCCA
    GCAGAACAGA GGGCTGGCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009204.1
    CDS140..3883
    Translation

    Target ORF information:

    RefSeq Version XM_025153436.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGATCC AGAATCTAAG GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA
    GCCTCCTCCA TCAGCACTAC AGTTAATGCA TCTGCTGTGA CCAAAGTTAA AGTCAAGAGA
    CATGAAATAA AGAGTGACCC AACTCCCCTG GGGTTTGAAG ATGCTTTTGA GAACACCATA
    TTGGCCCAGA CTAAATGTAG CACTGGTTCC ACCACAAGAC CCGTACCTAG ATCTGCTTCA
    CAGACAGGTT GGCCCAGTAG GCAAATGCCA TCTCTAGACA CATTTGAAGA CTTTGAAGCC
    ATTCCTTCCA GCATGGATGA ACTCTCCAAC TCTTCAGACA TGGAGGAAGA AACCAACCCT
    AACAATGGGA CCAATCTTTT TCGAGTAGAA GGCAGTTTTG ACAGCTGTTT CCCAGATTCT
    CTTACACTTG AAGATGGAGA TCTAGTGCAG TTTGTGCAGG AAGGAGAAAA TGGGCAATGG
    TTAGTGAAGA AATTGGTCTC GGAGAAAAGT GAATGGGTTC CTTCCAGTAT ATTGCAGCCA
    GCAGAGGGGG ACACTAACAA CATAATTAAG AACAGCAATG CAGACATGTA CAATGATTCC
    TGCAGCACGG CTTCAGTTGA GTCGGACACC AAGGAGAGTG AGGTGGCTAA AAGCTTCCCA
    GCAGAACAGA GGGCTGGCAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa152686
    Clone ID Related Accession (Same CDS sequence) XM_025153438.1
    Accession Version XM_025153438.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGGT TGGCCCAGTA GGCAAATGCC ATCTCTAGAC
    ACATTTGAAG ACTTTGAAGC CATTCCTTCC AGCATGGATG AACTCTCCAA CTCTTCAGAC
    ATGGAGGAAG AAACCAACCC TAACAATGGG ACCAATCTTT TTCGAGTAGA AGGCAGTTTT
    GACAGCTGTT TCCCAGATTC TCTTACACTT GAAGATGGAG ATCTAGTGCA GTTTGTGCAG
    GAAGGAGAAA ATGGGCAATG GTTAGTGAAG AAATTGGTCT CGGAGAAAAG TGAATGGGTT
    CCTTCCAGTA TATTGCAGCC AGCAGAGGGG GACACTAACA ACATAATTAA GAACAGCAAT
    GCAGACATGT ACAATGATTC CTGCAGCACG GCTTCAGTTG AGTCGGACAC CAAGGAGAGT
    GAGGTGGCTA AAAGCTTCCC AGCAGAACAG AGGGCTGGCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009206.1
    CDS138..3722
    Translation

    Target ORF information:

    RefSeq Version XM_025153438.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153438.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGGT TGGCCCAGTA GGCAAATGCC ATCTCTAGAC
    ACATTTGAAG ACTTTGAAGC CATTCCTTCC AGCATGGATG AACTCTCCAA CTCTTCAGAC
    ATGGAGGAAG AAACCAACCC TAACAATGGG ACCAATCTTT TTCGAGTAGA AGGCAGTTTT
    GACAGCTGTT TCCCAGATTC TCTTACACTT GAAGATGGAG ATCTAGTGCA GTTTGTGCAG
    GAAGGAGAAA ATGGGCAATG GTTAGTGAAG AAATTGGTCT CGGAGAAAAG TGAATGGGTT
    CCTTCCAGTA TATTGCAGCC AGCAGAGGGG GACACTAACA ACATAATTAA GAACAGCAAT
    GCAGACATGT ACAATGATTC CTGCAGCACG GCTTCAGTTG AGTCGGACAC CAAGGAGAGT
    GAGGTGGCTA AAAGCTTCCC AGCAGAACAG AGGGCTGGCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39983
    Clone ID Related Accession (Same CDS sequence) XM_015291524.2 , XM_015291524.1
    Accession Version XM_015291524.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015291524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTGTGCA GTTCCCTGTG TGAAATGCTT TTTTGGTTAA AAGAAGAAGA GATGTCTCAT 
    CAGGAGCTCA CCCAGCGACT GACTACAGTT ATCACCCATG TGGATGAAAT CATGCAGCAG
    GAGATACGAC CGCTGGTTGC AGTGGATATA ATAGAACAGC TACACAGGCA ATTTGCTATC
    TTGTCAGGAG GAAGAGGGAA GGATGGAGCG CCAATTATTA CCTTCCCAGA GTTTGCAGGA
    TTCAGTGAAA TACCCGATGA GGATTTTCTG AATGTGGTCA CGTACCTAAC CAGCATTCCC
    AGTCTGGAGG CTGCCAGCAT TGGATTCATC ATCATCATAG ACAGACGACG AGACAAATGG
    AGCGCAGTCA AGGCATCCCT GACACGGATA GCAGGAGCAT TTCCAGGAAA CCTACAGCTG
    GTGTTTGTCC TGCGTCCCTC ACGCTTTATC CAAAGGGCAA TCGCTGACAT TGGCATTAAA
    CTCTATCGAG ATGACTTTAA AATGAAGGTA CCGATCATCA TGCTAAATTC TGTCTCTGAT
    CTTCATGGCT ATATTGATAA ATGTCAACTG ACCCGGGAGC TGGGAGGAAC CCTGGAGTAC
    GGCCACAGTC AGTGGATACA TCACCGAACT GCTATTGAAA ACTTTGCAAT GACAGTGAAA
    ACAACAGCAC AGATGCTACA GACCTTTGGA ACGGACTTAG CAGAGACTGA ACTTCCCAAT
    GATGTGCAGT GTACAGAGGA GCTCCTATCT GCACACACTG ACCACCATAG CAAGCTGAAG
    GATGAGCTGA AACTAGCTGT GAAGCAGGGG GCCACACTAC TGACGTGCAT TAGGGAGCCA
    GTCACCCGAA GTGCCAACAG CAAGCTCAGT CCAGATGAAC TGGAAAACGT GGCAACAGTG
    GAAAGGTTGT TGGCTCAGTT GGATGAAACA GAAAAGGCTT TTGATCAATT TTGGACCAAG
    CATCACCTAA AACTAGAACA ATGCTTGCAG CTTCGACACT TTGAACATGA TTTTAGAGAA
    GTAAAATTAA CATTGGATAA TCTCATGGAA GCACAAGCTG GTTTTTCTGA CGTTGGAGAC
    AGTGTGACTC GAGTAGAACA CCTCCTAAGG GAACAGAAGC AACTGGAAGA AAAAGGGCAG
    GAACCTTTGG AGAAAGCCCA GACTCTAGCT CTCCATGGAG AACAGCTCAT CCAAAACAAC
    CATTATGCAG TTGACTCCAT TAGACCAAAG TGTGTTGAAC TCAGGCGTAT TTGCGATGAC
    TTTACTAATG AGACCAAGAA AAAGTATGAT ATTTTGGGGA AGTCTCTTGA GCTGCATAAG
    CAATTAGATA AGGCAAGCCA ATGGTGTGAA GCTGGAATCT ACCTCTTAGC TTCCCAAGCT
    GTGGACAAGT GCCAGTCACA AGAGGGAGCT GAAACTGCAC TCGTTGAAAT AGAGAAGTTC
    CTGGCAACAG CAAAGGAGCA CCAACTCTCT AACCCAAAGG AGTTCTACAA TCAGTTTGAC
    ACGATTCTCA CACCTGAAAT AAAGGCCAAC GCCCAAAGGA TTTTACAAAA GCTAGAAGAT
    GTACAAGAGA TGTTTGACAA GAGGCAAGTG AGTCTGAAGA AGCTAGCAGC CAAACAGACC
    CGTCCAGTGC AACCTGTTGC CCCACATCCT GAATCTTCCC CAAAACGAGC ATCACCAAAG
    ACTACCAGAC CAGCAGGAGT AGCTACCAAC AGGAGGTCCG CAGAAATTAC TTGTCCTCCA
    AAGTCCATCT CTGAGGCAGA GTTATCAAAA AAGAAAAGCA TTAAGAAGAC TAAAGGTGGA
    ATTAAGATTG AAGTGATGCA TGAAGCGAGC CAAGGGGGAT CTAGCAGAGT CTTGGTTATG
    AGTGAAAGCG ATGAGAACCT GTCGACGAGG AGGAGCCACA TAATCAATGA GCTGATAGAA
    ACCGAACGCG TTTATGTGGA AGAACTTCAA AGCATAATAG AGGGATATGC TTCAGAAATG
    GACAATCCAA GTTTATTACA TCTGATTCCA TCAGTGCTCC AGAACAAAAA GGATATTCTA
    TTTGGGAACC TCCCAGAAAT CTATGAATTC CACAACAGGA TTTTCCTTAA AGAGTTAGAA
    AATTGCATTG AAAATCCTGA GCTCCTTGCC CGATGCTTTT TGAAAAGGAA AGAGGACCTT
    CAAATATATG AAAAATACTG TCAGAACAAG CCAAGGTCTG AAGCTCTCTG GAGACAGTGT
    GGAGACAGCA TCTTTTTTCA GGAATGTCAG CGTAAATTGG ATCATAAGCT CTCACTAGAT
    GCGTATCTTT TAAAGCCAGT CCAGAGAATC ACCAAGTACC AGCTACTATT GAAGGAAATG
    CTGAAATGTA GCAAGAATTC AGAAGGTACT GCTGAACTGG AAGAGGCCTT GGCCACAGTG
    CTTGATATCA TCAAATCCGT AAATGACTCC ATGCACCAGA TAGCAATCAC AGGATATGAG
    GGGGACGTAA GTGAGCTTGG TAAGCTGTTG ATGCAAGGTT CCTTCAACGT GTGGACTGAC
    CACAAGAAGG GTCACAACAA GGTGAAGGAC TTGGCAAGAT TCAAGCCGAT GCAGAGACAC
    CTCTTCCTCT ACACAAAGAT GCTGCTTTTT TGCAAGAAAC GAGAGGAAAA CACAGATGGT
    CATGAGAAGA CAGCTTCATA CAGCTTTAAA AATTCATTAA AGATGAGCAC AGTGGGCATC
    ACAGAAAATG TAAAAGGAGA CAACAAGAAG TTTGAGATCT GGTATAATGG CAGAGAAGAA
    GTCTATATCA TTCAGGCATC TTCTGTTGAA CTGAAAAACA CTTGGATTTC CGAGATACGC
    AAAGTTCTAA CAGGACAACT GGAAGCATGT AGAGAAGCAA GTCAACTACA CCAAAGAATC
    ACCGAGAGTG TGTACCATGC ACCAATGAAT TCCTCCAATA TAACCAGGTA TAGCAGAAAG
    TCGTCAAGCA TATATGAAAA CAAATCCGAG ACATCAAACG TGGAAGCATC TAACAATAAA
    AACAGGAATT CCAGTCCCAG TGCCAGACAA AAGAGAGTTG ATGCTTCCAC CAGCCTTAAC
    CTTGAGCGCA CAGCGCTTGC TAGAAAGCGA TTCACATTGC AAGGTCTTTC CAACCGCAGA
    GCTCCACCCA AAGATCCAGA ATCTAAGGAA AGAGAAGTTG CCCGTCGCTT TTCCCTAGCC
    TCCTCCATCA GCACTACAGT TAATGCATCT GCTGTGACCA AAGGTCCCCT TGTTCCTGCA
    GTTAAAGTCA AGAGACATGA AATAAAGAGT GACCCAACTC CCCTGGGGTT TGAAGATGCT
    TTTGAGAACA CCATATTGGC CCAGACTAAA TGTAGCACTG GTTCCACCAC AAGACCCGTA
    CCTAGATCTG CTTCACAGAC AGGTTGGCCC AGTAGGCAAA TGCCATCTCT AGACACATTT
    GAAGACTTTG AAGCCATTCC TTCCAGCATG GATGAACTCT CCAACTCTTC AGACATGGAG
    GAAGAAACCA ACCCTAACAA TGGGACCAAT CTTTTTCGAG TAGAAGGCAG TTTTGACAGC
    TGTTTCCCAG ATTCTCTTAC ACTTGAAGAT GGAGATCTAG TGCAGTTTGT GCAGGAAGGA
    GAAAATGGGC AATGGTTAGT GAAGAAATTG GTCTCGGAGA AAAGTGAATG GGTTCCTTCC
    AGTATATTGC AGCCAGCAGA GGGGGACACT AACAACATAA TTAAGAACAG CAATGCAGAC
    ATGTACAATG ATTCCTGCAG CACGGCTTCA GTTGAGTCGG ACACCAAGGA GAGTGAGGTG
    GCTAAAAGCT TCCCAGCAGA ACAGAGGGCT GGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147010.1
    CDS462..4280
    Translation

    Target ORF information:

    RefSeq Version XM_015291524.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291524.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTGTGCA GTTCCCTGTG TGAAATGCTT TTTTGGTTAA AAGAAGAAGA GATGTCTCAT 
    CAGGAGCTCA CCCAGCGACT GACTACAGTT ATCACCCATG TGGATGAAAT CATGCAGCAG
    GAGATACGAC CGCTGGTTGC AGTGGATATA ATAGAACAGC TACACAGGCA ATTTGCTATC
    TTGTCAGGAG GAAGAGGGAA GGATGGAGCG CCAATTATTA CCTTCCCAGA GTTTGCAGGA
    TTCAGTGAAA TACCCGATGA GGATTTTCTG AATGTGGTCA CGTACCTAAC CAGCATTCCC
    AGTCTGGAGG CTGCCAGCAT TGGATTCATC ATCATCATAG ACAGACGACG AGACAAATGG
    AGCGCAGTCA AGGCATCCCT GACACGGATA GCAGGAGCAT TTCCAGGAAA CCTACAGCTG
    GTGTTTGTCC TGCGTCCCTC ACGCTTTATC CAAAGGGCAA TCGCTGACAT TGGCATTAAA
    CTCTATCGAG ATGACTTTAA AATGAAGGTA CCGATCATCA TGCTAAATTC TGTCTCTGAT
    CTTCATGGCT ATATTGATAA ATGTCAACTG ACCCGGGAGC TGGGAGGAAC CCTGGAGTAC
    GGCCACAGTC AGTGGATACA TCACCGAACT GCTATTGAAA ACTTTGCAAT GACAGTGAAA
    ACAACAGCAC AGATGCTACA GACCTTTGGA ACGGACTTAG CAGAGACTGA ACTTCCCAAT
    GATGTGCAGT GTACAGAGGA GCTCCTATCT GCACACACTG ACCACCATAG CAAGCTGAAG
    GATGAGCTGA AACTAGCTGT GAAGCAGGGG GCCACACTAC TGACGTGCAT TAGGGAGCCA
    GTCACCCGAA GTGCCAACAG CAAGCTCAGT CCAGATGAAC TGGAAAACGT GGCAACAGTG
    GAAAGGTTGT TGGCTCAGTT GGATGAAACA GAAAAGGCTT TTGATCAATT TTGGACCAAG
    CATCACCTAA AACTAGAACA ATGCTTGCAG CTTCGACACT TTGAACATGA TTTTAGAGAA
    GTAAAATTAA CATTGGATAA TCTCATGGAA GCACAAGCTG GTTTTTCTGA CGTTGGAGAC
    AGTGTGACTC GAGTAGAACA CCTCCTAAGG GAACAGAAGC AACTGGAAGA AAAAGGGCAG
    GAACCTTTGG AGAAAGCCCA GACTCTAGCT CTCCATGGAG AACAGCTCAT CCAAAACAAC
    CATTATGCAG TTGACTCCAT TAGACCAAAG TGTGTTGAAC TCAGGCGTAT TTGCGATGAC
    TTTACTAATG AGACCAAGAA AAAGTATGAT ATTTTGGGGA AGTCTCTTGA GCTGCATAAG
    CAATTAGATA AGGCAAGCCA ATGGTGTGAA GCTGGAATCT ACCTCTTAGC TTCCCAAGCT
    GTGGACAAGT GCCAGTCACA AGAGGGAGCT GAAACTGCAC TCGTTGAAAT AGAGAAGTTC
    CTGGCAACAG CAAAGGAGCA CCAACTCTCT AACCCAAAGG AGTTCTACAA TCAGTTTGAC
    ACGATTCTCA CACCTGAAAT AAAGGCCAAC GCCCAAAGGA TTTTACAAAA GCTAGAAGAT
    GTACAAGAGA TGTTTGACAA GAGGCAAGTG AGTCTGAAGA AGCTAGCAGC CAAACAGACC
    CGTCCAGTGC AACCTGTTGC CCCACATCCT GAATCTTCCC CAAAACGAGC ATCACCAAAG
    ACTACCAGAC CAGCAGGAGT AGCTACCAAC AGGAGGTCCG CAGAAATTAC TTGTCCTCCA
    AAGTCCATCT CTGAGGCAGA GTTATCAAAA AAGAAAAGCA TTAAGAAGAC TAAAGGTGGA
    ATTAAGATTG AAGTGATGCA TGAAGCGAGC CAAGGGGGAT CTAGCAGAGT CTTGGTTATG
    AGTGAAAGCG ATGAGAACCT GTCGACGAGG AGGAGCCACA TAATCAATGA GCTGATAGAA
    ACCGAACGCG TTTATGTGGA AGAACTTCAA AGCATAATAG AGGGATATGC TTCAGAAATG
    GACAATCCAA GTTTATTACA TCTGATTCCA TCAGTGCTCC AGAACAAAAA GGATATTCTA
    TTTGGGAACC TCCCAGAAAT CTATGAATTC CACAACAGGA TTTTCCTTAA AGAGTTAGAA
    AATTGCATTG AAAATCCTGA GCTCCTTGCC CGATGCTTTT TGAAAAGGAA AGAGGACCTT
    CAAATATATG AAAAATACTG TCAGAACAAG CCAAGGTCTG AAGCTCTCTG GAGACAGTGT
    GGAGACAGCA TCTTTTTTCA GGAATGTCAG CGTAAATTGG ATCATAAGCT CTCACTAGAT
    GCGTATCTTT TAAAGCCAGT CCAGAGAATC ACCAAGTACC AGCTACTATT GAAGGAAATG
    CTGAAATGTA GCAAGAATTC AGAAGGTACT GCTGAACTGG AAGAGGCCTT GGCCACAGTG
    CTTGATATCA TCAAATCCGT AAATGACTCC ATGCACCAGA TAGCAATCAC AGGATATGAG
    GGGGACGTAA GTGAGCTTGG TAAGCTGTTG ATGCAAGGTT CCTTCAACGT GTGGACTGAC
    CACAAGAAGG GTCACAACAA GGTGAAGGAC TTGGCAAGAT TCAAGCCGAT GCAGAGACAC
    CTCTTCCTCT ACACAAAGAT GCTGCTTTTT TGCAAGAAAC GAGAGGAAAA CACAGATGGT
    CATGAGAAGA CAGCTTCATA CAGCTTTAAA AATTCATTAA AGATGAGCAC AGTGGGCATC
    ACAGAAAATG TAAAAGGAGA CAACAAGAAG TTTGAGATCT GGTATAATGG CAGAGAAGAA
    GTCTATATCA TTCAGGCATC TTCTGTTGAA CTGAAAAACA CTTGGATTTC CGAGATACGC
    AAAGTTCTAA CAGGACAACT GGAAGCATGT AGAGAAGCAA GTCAACTACA CCAAAGAATC
    ACCGAGAGTG TGTACCATGC ACCAATGAAT TCCTCCAATA TAACCAGGTA TAGCAGAAAG
    TCGTCAAGCA TATATGAAAA CAAATCCGAG ACATCAAACG TGGAAGCATC TAACAATAAA
    AACAGGAATT CCAGTCCCAG TGCCAGACAA AAGAGAGTTG ATGCTTCCAC CAGCCTTAAC
    CTTGAGCGCA CAGCGCTTGC TAGAAAGCGA TTCACATTGC AAGGTCTTTC CAACCGCAGA
    GCTCCACCCA AAGATCCAGA ATCTAAGGAA AGAGAAGTTG CCCGTCGCTT TTCCCTAGCC
    TCCTCCATCA GCACTACAGT TAATGCATCT GCTGTGACCA AAGGTCCCCT TGTTCCTGCA
    GTTAAAGTCA AGAGACATGA AATAAAGAGT GACCCAACTC CCCTGGGGTT TGAAGATGCT
    TTTGAGAACA CCATATTGGC CCAGACTAAA TGTAGCACTG GTTCCACCAC AAGACCCGTA
    CCTAGATCTG CTTCACAGAC AGGTTGGCCC AGTAGGCAAA TGCCATCTCT AGACACATTT
    GAAGACTTTG AAGCCATTCC TTCCAGCATG GATGAACTCT CCAACTCTTC AGACATGGAG
    GAAGAAACCA ACCCTAACAA TGGGACCAAT CTTTTTCGAG TAGAAGGCAG TTTTGACAGC
    TGTTTCCCAG ATTCTCTTAC ACTTGAAGAT GGAGATCTAG TGCAGTTTGT GCAGGAAGGA
    GAAAATGGGC AATGGTTAGT GAAGAAATTG GTCTCGGAGA AAAGTGAATG GGTTCCTTCC
    AGTATATTGC AGCCAGCAGA GGGGGACACT AACAACATAA TTAAGAACAG CAATGCAGAC
    ATGTACAATG ATTCCTGCAG CACGGCTTCA GTTGAGTCGG ACACCAAGGA GAGTGAGGTG
    GCTAAAAGCT TCCCAGCAGA ACAGAGGGCT GGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa39983
    Clone ID Related Accession (Same CDS sequence) XM_015291524.2 , XM_015291524.1
    Accession Version XM_015291524.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3819bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTGTGCA GTTCCCTGTG TGAAATGCTT TTTTGGTTAA AAGAAGAAGA GATGTCTCAT 
    CAGGAGCTCA CCCAGCGACT GACTACAGTT ATCACCCATG TGGATGAAAT CATGCAGCAG
    GAGATACGAC CGCTGGTTGC AGTGGATATA ATAGAACAGC TACACAGGCA ATTTGCTATC
    TTGTCAGGAG GAAGAGGGAA GGATGGAGCG CCAATTATTA CCTTCCCAGA GTTTGCAGGA
    TTCAGTGAAA TACCCGATGA GGATTTTCTG AATGTGGTCA CGTACCTAAC CAGCATTCCC
    AGTCTGGAGG CTGCCAGCAT TGGATTCATC ATCATCATAG ACAGACGACG AGACAAATGG
    AGCGCAGTCA AGGCATCCCT GACACGGATA GCAGGAGCAT TTCCAGGAAA CCTACAGCTG
    GTGTTTGTCC TGCGTCCCTC ACGCTTTATC CAAAGGGCAA TCGCTGACAT TGGCATTAAA
    CTCTATCGAG ATGACTTTAA AATGAAGGTA CCGATCATCA TGCTAAATTC TGTCTCTGAT
    CTTCATGGCT ATATTGATAA ATGTCAACTG ACCCGGGAGC TGGGAGGAAC CCTGGAGTAC
    GGCCACAGTC AGTGGATACA TCACCGAACT GCTATTGAAA ACTTTGCAAT GACAGTGAAA
    ACAACAGCAC AGATGCTACA GACCTTTGGA ACGGACTTAG CAGAGACTGA ACTTCCCAAT
    GATGTGCAGT GTACAGAGGA GCTCCTATCT GCACACACTG ACCACCATAG CAAGCTGAAG
    GATGAGCTGA AACTAGCTGT GAAGCAGGGG GCCACACTAC TGACGTGCAT TAGGGAGCCA
    GTCACCCGAA GTGCCAACAG CAAGCTCAGT CCAGATGAAC TGGAAAACGT GGCAACAGTG
    GAAAGGTTGT TGGCTCAGTT GGATGAAACA GAAAAGGCTT TTGATCAATT TTGGACCAAG
    CATCACCTAA AACTAGAACA ATGCTTGCAG CTTCGACACT TTGAACATGA TTTTAGAGAA
    GTAAAATTAA CATTGGATAA TCTCATGGAA GCACAAGCTG GTTTTTCTGA CGTTGGAGAC
    AGTGTGACTC GAGTAGAACA CCTCCTAAGG GAACAGAAGC AACTGGAAGA AAAAGGGCAG
    GAACCTTTGG AGAAAGCCCA GACTCTAGCT CTCCATGGAG AACAGCTCAT CCAAAACAAC
    CATTATGCAG TTGACTCCAT TAGACCAAAG TGTGTTGAAC TCAGGCGTAT TTGCGATGAC
    TTTACTAATG AGACCAAGAA AAAGTATGAT ATTTTGGGGA AGTCTCTTGA GCTGCATAAG
    CAATTAGATA AGGCAAGCCA ATGGTGTGAA GCTGGAATCT ACCTCTTAGC TTCCCAAGCT
    GTGGACAAGT GCCAGTCACA AGAGGGAGCT GAAACTGCAC TCGTTGAAAT AGAGAAGTTC
    CTGGCAACAG CAAAGGAGCA CCAACTCTCT AACCCAAAGG AGTTCTACAA TCAGTTTGAC
    ACGATTCTCA CACCTGAAAT AAAGGCCAAC GCCCAAAGGA TTTTACAAAA GCTAGAAGAT
    GTACAAGAGA TGTTTGACAA GAGGCAAGTG AGTCTGAAGA AGCTAGCAGC CAAACAGACC
    CGTCCAGTGC AACCTGTTGC CCCACATCCT GAATCTTCCC CAAAACGAGC ATCACCAAAG
    ACTACCAGAC CAGCAGGAGT AGCTACCAAC AGGAGGTCCG CAGAAATTAC TTGTCCTCCA
    AAGTCCATCT CTGAGGCAGA GTTATCAAAA AAGAAAAGCA TTAAGAAGAC TAAAGGTGGA
    ATTAAGATTG AAGTGATGCA TGAAGCGAGC CAAGGGGGAT CTAGCAGAGT CTTGGTTATG
    AGTGAAAGCG ATGAGAACCT GTCGACGAGG AGGAGCCACA TAATCAATGA GCTGATAGAA
    ACCGAACGCG TTTATGTGGA AGAACTTCAA AGCATAATAG AGGGATATGC TTCAGAAATG
    GACAATCCAA GTTTATTACA TCTGATTCCA TCAGTGCTCC AGAACAAAAA GGATATTCTA
    TTTGGGAACC TCCCAGAAAT CTATGAATTC CACAACAGGA TTTTCCTTAA AGAGTTAGAA
    AATTGCATTG AAAATCCTGA GCTCCTTGCC CGATGCTTTT TGAAAAGGAA AGAGGACCTT
    CAAATATATG AAAAATACTG TCAGAACAAG CCAAGGTCTG AAGCTCTCTG GAGACAGTGT
    GGAGACAGCA TCTTTTTTCA GGAATGTCAG CGTAAATTGG ATCATAAGCT CTCACTAGAT
    GCGTATCTTT TAAAGCCAGT CCAGAGAATC ACCAAGTACC AGCTACTATT GAAGGAAATG
    CTGAAATGTA GCAAGAATTC AGAAGGTACT GCTGAACTGG AAGAGGCCTT GGCCACAGTG
    CTTGATATCA TCAAATCCGT AAATGACTCC ATGCACCAGA TAGCAATCAC AGGATATGAG
    GGGGACGTAA GTGAGCTTGG TAAGCTGTTG ATGCAAGGTT CCTTCAACGT GTGGACTGAC
    CACAAGAAGG GTCACAACAA GGTGAAGGAC TTGGCAAGAT TCAAGCCGAT GCAGAGACAC
    CTCTTCCTCT ACACAAAGAT GCTGCTTTTT TGCAAGAAAC GAGAGGAAAA CACAGATGGT
    CATGAGAAGA CAGCTTCATA CAGCTTTAAA AATTCATTAA AGATGAGCAC AGTGGGCATC
    ACAGAAAATG TAAAAGGAGA CAACAAGAAG TTTGAGATCT GGTATAATGG CAGAGAAGAA
    GTCTATATCA TTCAGGCATC TTCTGTTGAA CTGAAAAACA CTTGGATTTC CGAGATACGC
    AAAGTTCTAA CAGGACAACT GGAAGCATGT AGAGAAGCAA GTCAACTACA CCAAAGAATC
    ACCGAGAGTG TGTACCATGC ACCAATGAAT TCCTCCAATA TAACCAGGTA TAGCAGAAAG
    TCGTCAAGCA TATATGAAAA CAAATCCGAG ACATCAAACG TGGAAGCATC TAACAATAAA
    AACAGGAATT CCAGTCCCAG TGCCAGACAA AAGAGAGTTG ATGCTTCCAC CAGCCTTAAC
    CTTGAGCGCA CAGCGCTTGC TAGAAAGCGA TTCACATTGC AAGGTCTTTC CAACCGCAGA
    GCTCCACCCA AAGATCCAGA ATCTAAGGAA AGAGAAGTTG CCCGTCGCTT TTCCCTAGCC
    TCCTCCATCA GCACTACAGT TAATGCATCT GCTGTGACCA AAGGTCCCCT TGTTCCTGCA
    GTTAAAGTCA AGAGACATGA AATAAAGAGT GACCCAACTC CCCTGGGGTT TGAAGATGCT
    TTTGAGAACA CCATATTGGC CCAGACTAAA TGTAGCACTG GTTCCACCAC AAGACCCGTA
    CCTAGATCTG CTTCACAGAC AGGTTGGCCC AGTAGGCAAA TGCCATCTCT AGACACATTT
    GAAGACTTTG AAGCCATTCC TTCCAGCATG GATGAACTCT CCAACTCTTC AGACATGGAG
    GAAGAAACCA ACCCTAACAA TGGGACCAAT CTTTTTCGAG TAGAAGGCAG TTTTGACAGC
    TGTTTCCCAG ATTCTCTTAC ACTTGAAGAT GGAGATCTAG TGCAGTTTGT GCAGGAAGGA
    GAAAATGGGC AATGGTTAGT GAAGAAATTG GTCTCGGAGA AAAGTGAATG GGTTCCTTCC
    AGTATATTGC AGCCAGCAGA GGGGGACACT AACAACATAA TTAAGAACAG CAATGCAGAC
    ATGTACAATG ATTCCTGCAG CACGGCTTCA GTTGAGTCGG ACACCAAGGA GAGTGAGGTG
    GCTAAAAGCT TCCCAGCAGA ACAGAGGGCT GGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015147010.1
    CDS469..4287
    Translation

    Target ORF information:

    RefSeq Version XM_015291524.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015291524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTGTGCA GTTCCCTGTG TGAAATGCTT TTTTGGTTAA AAGAAGAAGA GATGTCTCAT 
    CAGGAGCTCA CCCAGCGACT GACTACAGTT ATCACCCATG TGGATGAAAT CATGCAGCAG
    GAGATACGAC CGCTGGTTGC AGTGGATATA ATAGAACAGC TACACAGGCA ATTTGCTATC
    TTGTCAGGAG GAAGAGGGAA GGATGGAGCG CCAATTATTA CCTTCCCAGA GTTTGCAGGA
    TTCAGTGAAA TACCCGATGA GGATTTTCTG AATGTGGTCA CGTACCTAAC CAGCATTCCC
    AGTCTGGAGG CTGCCAGCAT TGGATTCATC ATCATCATAG ACAGACGACG AGACAAATGG
    AGCGCAGTCA AGGCATCCCT GACACGGATA GCAGGAGCAT TTCCAGGAAA CCTACAGCTG
    GTGTTTGTCC TGCGTCCCTC ACGCTTTATC CAAAGGGCAA TCGCTGACAT TGGCATTAAA
    CTCTATCGAG ATGACTTTAA AATGAAGGTA CCGATCATCA TGCTAAATTC TGTCTCTGAT
    CTTCATGGCT ATATTGATAA ATGTCAACTG ACCCGGGAGC TGGGAGGAAC CCTGGAGTAC
    GGCCACAGTC AGTGGATACA TCACCGAACT GCTATTGAAA ACTTTGCAAT GACAGTGAAA
    ACAACAGCAC AGATGCTACA GACCTTTGGA ACGGACTTAG CAGAGACTGA ACTTCCCAAT
    GATGTGCAGT GTACAGAGGA GCTCCTATCT GCACACACTG ACCACCATAG CAAGCTGAAG
    GATGAGCTGA AACTAGCTGT GAAGCAGGGG GCCACACTAC TGACGTGCAT TAGGGAGCCA
    GTCACCCGAA GTGCCAACAG CAAGCTCAGT CCAGATGAAC TGGAAAACGT GGCAACAGTG
    GAAAGGTTGT TGGCTCAGTT GGATGAAACA GAAAAGGCTT TTGATCAATT TTGGACCAAG
    CATCACCTAA AACTAGAACA ATGCTTGCAG CTTCGACACT TTGAACATGA TTTTAGAGAA
    GTAAAATTAA CATTGGATAA TCTCATGGAA GCACAAGCTG GTTTTTCTGA CGTTGGAGAC
    AGTGTGACTC GAGTAGAACA CCTCCTAAGG GAACAGAAGC AACTGGAAGA AAAAGGGCAG
    GAACCTTTGG AGAAAGCCCA GACTCTAGCT CTCCATGGAG AACAGCTCAT CCAAAACAAC
    CATTATGCAG TTGACTCCAT TAGACCAAAG TGTGTTGAAC TCAGGCGTAT TTGCGATGAC
    TTTACTAATG AGACCAAGAA AAAGTATGAT ATTTTGGGGA AGTCTCTTGA GCTGCATAAG
    CAATTAGATA AGGCAAGCCA ATGGTGTGAA GCTGGAATCT ACCTCTTAGC TTCCCAAGCT
    GTGGACAAGT GCCAGTCACA AGAGGGAGCT GAAACTGCAC TCGTTGAAAT AGAGAAGTTC
    CTGGCAACAG CAAAGGAGCA CCAACTCTCT AACCCAAAGG AGTTCTACAA TCAGTTTGAC
    ACGATTCTCA CACCTGAAAT AAAGGCCAAC GCCCAAAGGA TTTTACAAAA GCTAGAAGAT
    GTACAAGAGA TGTTTGACAA GAGGCAAGTG AGTCTGAAGA AGCTAGCAGC CAAACAGACC
    CGTCCAGTGC AACCTGTTGC CCCACATCCT GAATCTTCCC CAAAACGAGC ATCACCAAAG
    ACTACCAGAC CAGCAGGAGT AGCTACCAAC AGGAGGTCCG CAGAAATTAC TTGTCCTCCA
    AAGTCCATCT CTGAGGCAGA GTTATCAAAA AAGAAAAGCA TTAAGAAGAC TAAAGGTGGA
    ATTAAGATTG AAGTGATGCA TGAAGCGAGC CAAGGGGGAT CTAGCAGAGT CTTGGTTATG
    AGTGAAAGCG ATGAGAACCT GTCGACGAGG AGGAGCCACA TAATCAATGA GCTGATAGAA
    ACCGAACGCG TTTATGTGGA AGAACTTCAA AGCATAATAG AGGGATATGC TTCAGAAATG
    GACAATCCAA GTTTATTACA TCTGATTCCA TCAGTGCTCC AGAACAAAAA GGATATTCTA
    TTTGGGAACC TCCCAGAAAT CTATGAATTC CACAACAGGA TTTTCCTTAA AGAGTTAGAA
    AATTGCATTG AAAATCCTGA GCTCCTTGCC CGATGCTTTT TGAAAAGGAA AGAGGACCTT
    CAAATATATG AAAAATACTG TCAGAACAAG CCAAGGTCTG AAGCTCTCTG GAGACAGTGT
    GGAGACAGCA TCTTTTTTCA GGAATGTCAG CGTAAATTGG ATCATAAGCT CTCACTAGAT
    GCGTATCTTT TAAAGCCAGT CCAGAGAATC ACCAAGTACC AGCTACTATT GAAGGAAATG
    CTGAAATGTA GCAAGAATTC AGAAGGTACT GCTGAACTGG AAGAGGCCTT GGCCACAGTG
    CTTGATATCA TCAAATCCGT AAATGACTCC ATGCACCAGA TAGCAATCAC AGGATATGAG
    GGGGACGTAA GTGAGCTTGG TAAGCTGTTG ATGCAAGGTT CCTTCAACGT GTGGACTGAC
    CACAAGAAGG GTCACAACAA GGTGAAGGAC TTGGCAAGAT TCAAGCCGAT GCAGAGACAC
    CTCTTCCTCT ACACAAAGAT GCTGCTTTTT TGCAAGAAAC GAGAGGAAAA CACAGATGGT
    CATGAGAAGA CAGCTTCATA CAGCTTTAAA AATTCATTAA AGATGAGCAC AGTGGGCATC
    ACAGAAAATG TAAAAGGAGA CAACAAGAAG TTTGAGATCT GGTATAATGG CAGAGAAGAA
    GTCTATATCA TTCAGGCATC TTCTGTTGAA CTGAAAAACA CTTGGATTTC CGAGATACGC
    AAAGTTCTAA CAGGACAACT GGAAGCATGT AGAGAAGCAA GTCAACTACA CCAAAGAATC
    ACCGAGAGTG TGTACCATGC ACCAATGAAT TCCTCCAATA TAACCAGGTA TAGCAGAAAG
    TCGTCAAGCA TATATGAAAA CAAATCCGAG ACATCAAACG TGGAAGCATC TAACAATAAA
    AACAGGAATT CCAGTCCCAG TGCCAGACAA AAGAGAGTTG ATGCTTCCAC CAGCCTTAAC
    CTTGAGCGCA CAGCGCTTGC TAGAAAGCGA TTCACATTGC AAGGTCTTTC CAACCGCAGA
    GCTCCACCCA AAGATCCAGA ATCTAAGGAA AGAGAAGTTG CCCGTCGCTT TTCCCTAGCC
    TCCTCCATCA GCACTACAGT TAATGCATCT GCTGTGACCA AAGGTCCCCT TGTTCCTGCA
    GTTAAAGTCA AGAGACATGA AATAAAGAGT GACCCAACTC CCCTGGGGTT TGAAGATGCT
    TTTGAGAACA CCATATTGGC CCAGACTAAA TGTAGCACTG GTTCCACCAC AAGACCCGTA
    CCTAGATCTG CTTCACAGAC AGGTTGGCCC AGTAGGCAAA TGCCATCTCT AGACACATTT
    GAAGACTTTG AAGCCATTCC TTCCAGCATG GATGAACTCT CCAACTCTTC AGACATGGAG
    GAAGAAACCA ACCCTAACAA TGGGACCAAT CTTTTTCGAG TAGAAGGCAG TTTTGACAGC
    TGTTTCCCAG ATTCTCTTAC ACTTGAAGAT GGAGATCTAG TGCAGTTTGT GCAGGAAGGA
    GAAAATGGGC AATGGTTAGT GAAGAAATTG GTCTCGGAGA AAAGTGAATG GGTTCCTTCC
    AGTATATTGC AGCCAGCAGA GGGGGACACT AACAACATAA TTAAGAACAG CAATGCAGAC
    ATGTACAATG ATTCCTGCAG CACGGCTTCA GTTGAGTCGG ACACCAAGGA GAGTGAGGTG
    GCTAAAAGCT TCCCAGCAGA ACAGAGGGCT GGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12709
    Clone ID Related Accession (Same CDS sequence) XM_004943437.2 , XM_004943437.3
    Accession Version XM_004943437.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGGTCCCCTT GTTCCTGCAG TTAAAGTCAA GAGACATGAA
    ATAAAGAGTG ACCCAACTCC CCTGGGGTTT GAAGATGCTT TTGAGAACAC CATATTGGCC
    CAGACTAAAT GTAGCACTGG TTCCACCACA AGACCCGTAC CTAGATCTGC TTCACAGACA
    GGTTGGCCCA GTAGGCAAAT GCCATCTCTA GACACATTTG AAGACTTTGA AGCCATTCCT
    TCCAGCATGG ATGAACTCTC CAACTCTTCA GACATGGAGG AAGAAACCAA CCCTAACAAT
    GGGACCAATC TTTTTCGAGT AGAAGGCAGT TTTGACAGCT GTTTCCCAGA TTCTCTTACA
    CTTGAAGATG GAGATCTAGT GCAGTTTGTG CAGGAAGGAG AAAATGGGCA ATGGTTAGTG
    AAGAAATTGG TCTCGGAGAA AAGTGAATGG GTTCCTTCCA GTATATTGCA GCCAGCAGAG
    GGGGACACTA ACAACATAAT TAAGAACAGC AATGCAGACA TGTACAATGA TTCCTGCAGC
    ACGGCTTCAG TTGAGTCGGA CACCAAGGAG AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA
    CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943494.1
    CDS156..4013
    Translation

    Target ORF information:

    RefSeq Version XM_004943437.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943437.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGGTCCCCTT GTTCCTGCAG TTAAAGTCAA GAGACATGAA
    ATAAAGAGTG ACCCAACTCC CCTGGGGTTT GAAGATGCTT TTGAGAACAC CATATTGGCC
    CAGACTAAAT GTAGCACTGG TTCCACCACA AGACCCGTAC CTAGATCTGC TTCACAGACA
    GGTTGGCCCA GTAGGCAAAT GCCATCTCTA GACACATTTG AAGACTTTGA AGCCATTCCT
    TCCAGCATGG ATGAACTCTC CAACTCTTCA GACATGGAGG AAGAAACCAA CCCTAACAAT
    GGGACCAATC TTTTTCGAGT AGAAGGCAGT TTTGACAGCT GTTTCCCAGA TTCTCTTACA
    CTTGAAGATG GAGATCTAGT GCAGTTTGTG CAGGAAGGAG AAAATGGGCA ATGGTTAGTG
    AAGAAATTGG TCTCGGAGAA AAGTGAATGG GTTCCTTCCA GTATATTGCA GCCAGCAGAG
    GGGGACACTA ACAACATAAT TAAGAACAGC AATGCAGACA TGTACAATGA TTCCTGCAGC
    ACGGCTTCAG TTGAGTCGGA CACCAAGGAG AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA
    CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12709
    Clone ID Related Accession (Same CDS sequence) XM_004943437.2 , XM_004943437.3
    Accession Version XM_004943437.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3858bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGGTCCCCTT GTTCCTGCAG TTAAAGTCAA GAGACATGAA
    ATAAAGAGTG ACCCAACTCC CCTGGGGTTT GAAGATGCTT TTGAGAACAC CATATTGGCC
    CAGACTAAAT GTAGCACTGG TTCCACCACA AGACCCGTAC CTAGATCTGC TTCACAGACA
    GGTTGGCCCA GTAGGCAAAT GCCATCTCTA GACACATTTG AAGACTTTGA AGCCATTCCT
    TCCAGCATGG ATGAACTCTC CAACTCTTCA GACATGGAGG AAGAAACCAA CCCTAACAAT
    GGGACCAATC TTTTTCGAGT AGAAGGCAGT TTTGACAGCT GTTTCCCAGA TTCTCTTACA
    CTTGAAGATG GAGATCTAGT GCAGTTTGTG CAGGAAGGAG AAAATGGGCA ATGGTTAGTG
    AAGAAATTGG TCTCGGAGAA AAGTGAATGG GTTCCTTCCA GTATATTGCA GCCAGCAGAG
    GGGGACACTA ACAACATAAT TAAGAACAGC AATGCAGACA TGTACAATGA TTCCTGCAGC
    ACGGCTTCAG TTGAGTCGGA CACCAAGGAG AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA
    CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943494.1
    CDS140..3997
    Translation

    Target ORF information:

    RefSeq Version XM_004943437.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGGTCCCCTT GTTCCTGCAG TTAAAGTCAA GAGACATGAA
    ATAAAGAGTG ACCCAACTCC CCTGGGGTTT GAAGATGCTT TTGAGAACAC CATATTGGCC
    CAGACTAAAT GTAGCACTGG TTCCACCACA AGACCCGTAC CTAGATCTGC TTCACAGACA
    GGTTGGCCCA GTAGGCAAAT GCCATCTCTA GACACATTTG AAGACTTTGA AGCCATTCCT
    TCCAGCATGG ATGAACTCTC CAACTCTTCA GACATGGAGG AAGAAACCAA CCCTAACAAT
    GGGACCAATC TTTTTCGAGT AGAAGGCAGT TTTGACAGCT GTTTCCCAGA TTCTCTTACA
    CTTGAAGATG GAGATCTAGT GCAGTTTGTG CAGGAAGGAG AAAATGGGCA ATGGTTAGTG
    AAGAAATTGG TCTCGGAGAA AAGTGAATGG GTTCCTTCCA GTATATTGCA GCCAGCAGAG
    GGGGACACTA ACAACATAAT TAAGAACAGC AATGCAGACA TGTACAATGA TTCCTGCAGC
    ACGGCTTCAG TTGAGTCGGA CACCAAGGAG AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA
    CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12710
    Clone ID Related Accession (Same CDS sequence) XM_004943438.3 , XM_004943438.2
    Accession Version XM_004943438.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGATCA TCATGCTAAA TTCTGTCTCT GATCTTCATG GCTATATTGA TAAATGTCAA
    CTGACCCGGG AGCTGGGAGG AACCCTGGAG TACGGCCACA GTCAGTGGAT ACATCACCGA
    ACTGCTATTG AAAACTTTGC AATGACAGTG AAAACAACAG CACAGATGCT ACAGACCTTT
    GGAACGGACT TAGCAGAGAC TGAACTTCCC AATGATGTGC AGTGTACAGA GGAGCTCCTA
    TCTGCACACA CTGACCACCA TAGCAAGCTG AAGGATGAGC TGAAACTAGC TGTGAAGCAG
    GGGGCCACAC TACTGACGTG CATTAGGGAG CCAGTCACCC GAAGTGCCAA CAGCAAGCTC
    AGTCCAGATG AACTGGAAAA CGTGGCAACA GTGGAAAGGT TGTTGGCTCA GTTGGATGAA
    ACAGAAAAGG CTTTTGATCA ATTTTGGACC AAGCATCACC TAAAACTAGA ACAATGCTTG
    CAGCTTCGAC ACTTTGAACA TGATTTTAGA GAAGTAAAAT TAACATTGGA TAATCTCATG
    GAAGCACAAG CTGGTTTTTC TGACGTTGGA GACAGTGTGA CTCGAGTAGA ACACCTCCTA
    AGGGAACAGA AGCAACTGGA AGAAAAAGGG CAGGAACCTT TGGAGAAAGC CCAGACTCTA
    GCTCTCCATG GAGAACAGCT CATCCAAAAC AACCATTATG CAGTTGACTC CATTAGACCA
    AAGTGTGTTG AACTCAGGCG TATTTGCGAT GACTTTACTA ATGAGACCAA GAAAAAGTAT
    GATATTTTGG GGAAGTCTCT TGAGCTGCAT AAGCAATTAG ATAAGGCAAG CCAATGGTGT
    GAAGCTGGAA TCTACCTCTT AGCTTCCCAA GCTGTGGACA AGTGCCAGTC ACAAGAGGGA
    GCTGAAACTG CACTCGTTGA AATAGAGAAG TTCCTGGCAA CAGCAAAGGA GCACCAACTC
    TCTAACCCAA AGGAGTTCTA CAATCAGTTT GACACGATTC TCACACCTGA AATAAAGGCC
    AACGCCCAAA GGATTTTACA AAAGCTAGAA GATGTACAAG AGATGTTTGA CAAGAGGCAA
    GTGAGTCTGA AGAAGCTAGC AGCCAAACAG ACCCGTCCAG TGCAACCTGT TGCCCCACAT
    CCTGAATCTT CCCCAAAACG AGCATCACCA AAGACTACCA GACCAGCAGG AGTAGCTACC
    AACAGGAGGT CCGCAGAAAT TACTTGTCCT CCAAAGTCCA TCTCTGAGGC AGAGTTATCA
    AAAAAGAAAA GCATTAAGAA GACTAAAGGT GGAATTAAGA TTGAAGTGAT GCATGAAGCG
    AGCCAAGGGG GATCTAGCAG AGTCTTGGTT ATGAGTGAAA GCGATGAGAA CCTGTCGACG
    AGGAGGAGCC ACATAATCAA TGAGCTGATA GAAACCGAAC GCGTTTATGT GGAAGAACTT
    CAAAGCATAA TAGAGGGATA TGCTTCAGAA ATGGACAATC CAAGTTTATT ACATCTGATT
    CCATCAGTGC TCCAGAACAA AAAGGATATT CTATTTGGGA ACCTCCCAGA AATCTATGAA
    TTCCACAACA GGATTTTCCT TAAAGAGTTA GAAAATTGCA TTGAAAATCC TGAGCTCCTT
    GCCCGATGCT TTTTGAAAAG GAAAGAGGAC CTTCAAATAT ATGAAAAATA CTGTCAGAAC
    AAGCCAAGGT CTGAAGCTCT CTGGAGACAG TGTGGAGACA GCATCTTTTT TCAGGAATGT
    CAGCGTAAAT TGGATCATAA GCTCTCACTA GATGCGTATC TTTTAAAGCC AGTCCAGAGA
    ATCACCAAGT ACCAGCTACT ATTGAAGGAA ATGCTGAAAT GTAGCAAGAA TTCAGAAGGT
    ACTGCTGAAC TGGAAGAGGC CTTGGCCACA GTGCTTGATA TCATCAAATC CGTAAATGAC
    TCCATGCACC AGATAGCAAT CACAGGATAT GAGGGGGACG TAAGTGAGCT TGGTAAGCTG
    TTGATGCAAG GTTCCTTCAA CGTGTGGACT GACCACAAGA AGGGTCACAA CAAGGTGAAG
    GACTTGGCAA GATTCAAGCC GATGCAGAGA CACCTCTTCC TCTACACAAA GATGCTGCTT
    TTTTGCAAGA AACGAGAGGA AAACACAGAT GGTCATGAGA AGACAGCTTC ATACAGCTTT
    AAAAATTCAT TAAAGATGAG CACAGTGGGC ATCACAGAAA ATGTAAAAGG AGACAACAAG
    AAGTTTGAGA TCTGGTATAA TGGCAGAGAA GAAGTCTATA TCATTCAGGC ATCTTCTGTT
    GAACTGAAAA ACACTTGGAT TTCCGAGATA CGCAAAGTTC TAACAGGACA ACTGGAAGCA
    TGTAGAGAAG CAAGTCAACT ACACCAAAGA ATCACCGAGA GTGTGTACCA TGCACCAATG
    AATTCCTCCA ATATAACCAG GTATAGCAGA AAGTCGTCAA GCATATATGA AAACAAATCC
    GAGACATCAA ACGTGGAAGC ATCTAACAAT AAAAACAGGA ATTCCAGTCC CAGTGCCAGA
    CAAAAGAGAG TTGATGCTTC CACCAGCCTT AACCTTGAGC GCACAGCGCT TGCTAGAAAG
    CGATTCACAT TGCAAGGTCT TTCCAACCGC AGAGCTCCAC CCAAAGATCC AGAATCTAAG
    GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA GCCTCCTCCA TCAGCACTAC AGTTAATGCA
    TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943495.1
    CDS140..3991
    Translation

    Target ORF information:

    RefSeq Version XM_004943438.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943438.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGATCA TCATGCTAAA TTCTGTCTCT GATCTTCATG GCTATATTGA TAAATGTCAA
    CTGACCCGGG AGCTGGGAGG AACCCTGGAG TACGGCCACA GTCAGTGGAT ACATCACCGA
    ACTGCTATTG AAAACTTTGC AATGACAGTG AAAACAACAG CACAGATGCT ACAGACCTTT
    GGAACGGACT TAGCAGAGAC TGAACTTCCC AATGATGTGC AGTGTACAGA GGAGCTCCTA
    TCTGCACACA CTGACCACCA TAGCAAGCTG AAGGATGAGC TGAAACTAGC TGTGAAGCAG
    GGGGCCACAC TACTGACGTG CATTAGGGAG CCAGTCACCC GAAGTGCCAA CAGCAAGCTC
    AGTCCAGATG AACTGGAAAA CGTGGCAACA GTGGAAAGGT TGTTGGCTCA GTTGGATGAA
    ACAGAAAAGG CTTTTGATCA ATTTTGGACC AAGCATCACC TAAAACTAGA ACAATGCTTG
    CAGCTTCGAC ACTTTGAACA TGATTTTAGA GAAGTAAAAT TAACATTGGA TAATCTCATG
    GAAGCACAAG CTGGTTTTTC TGACGTTGGA GACAGTGTGA CTCGAGTAGA ACACCTCCTA
    AGGGAACAGA AGCAACTGGA AGAAAAAGGG CAGGAACCTT TGGAGAAAGC CCAGACTCTA
    GCTCTCCATG GAGAACAGCT CATCCAAAAC AACCATTATG CAGTTGACTC CATTAGACCA
    AAGTGTGTTG AACTCAGGCG TATTTGCGAT GACTTTACTA ATGAGACCAA GAAAAAGTAT
    GATATTTTGG GGAAGTCTCT TGAGCTGCAT AAGCAATTAG ATAAGGCAAG CCAATGGTGT
    GAAGCTGGAA TCTACCTCTT AGCTTCCCAA GCTGTGGACA AGTGCCAGTC ACAAGAGGGA
    GCTGAAACTG CACTCGTTGA AATAGAGAAG TTCCTGGCAA CAGCAAAGGA GCACCAACTC
    TCTAACCCAA AGGAGTTCTA CAATCAGTTT GACACGATTC TCACACCTGA AATAAAGGCC
    AACGCCCAAA GGATTTTACA AAAGCTAGAA GATGTACAAG AGATGTTTGA CAAGAGGCAA
    GTGAGTCTGA AGAAGCTAGC AGCCAAACAG ACCCGTCCAG TGCAACCTGT TGCCCCACAT
    CCTGAATCTT CCCCAAAACG AGCATCACCA AAGACTACCA GACCAGCAGG AGTAGCTACC
    AACAGGAGGT CCGCAGAAAT TACTTGTCCT CCAAAGTCCA TCTCTGAGGC AGAGTTATCA
    AAAAAGAAAA GCATTAAGAA GACTAAAGGT GGAATTAAGA TTGAAGTGAT GCATGAAGCG
    AGCCAAGGGG GATCTAGCAG AGTCTTGGTT ATGAGTGAAA GCGATGAGAA CCTGTCGACG
    AGGAGGAGCC ACATAATCAA TGAGCTGATA GAAACCGAAC GCGTTTATGT GGAAGAACTT
    CAAAGCATAA TAGAGGGATA TGCTTCAGAA ATGGACAATC CAAGTTTATT ACATCTGATT
    CCATCAGTGC TCCAGAACAA AAAGGATATT CTATTTGGGA ACCTCCCAGA AATCTATGAA
    TTCCACAACA GGATTTTCCT TAAAGAGTTA GAAAATTGCA TTGAAAATCC TGAGCTCCTT
    GCCCGATGCT TTTTGAAAAG GAAAGAGGAC CTTCAAATAT ATGAAAAATA CTGTCAGAAC
    AAGCCAAGGT CTGAAGCTCT CTGGAGACAG TGTGGAGACA GCATCTTTTT TCAGGAATGT
    CAGCGTAAAT TGGATCATAA GCTCTCACTA GATGCGTATC TTTTAAAGCC AGTCCAGAGA
    ATCACCAAGT ACCAGCTACT ATTGAAGGAA ATGCTGAAAT GTAGCAAGAA TTCAGAAGGT
    ACTGCTGAAC TGGAAGAGGC CTTGGCCACA GTGCTTGATA TCATCAAATC CGTAAATGAC
    TCCATGCACC AGATAGCAAT CACAGGATAT GAGGGGGACG TAAGTGAGCT TGGTAAGCTG
    TTGATGCAAG GTTCCTTCAA CGTGTGGACT GACCACAAGA AGGGTCACAA CAAGGTGAAG
    GACTTGGCAA GATTCAAGCC GATGCAGAGA CACCTCTTCC TCTACACAAA GATGCTGCTT
    TTTTGCAAGA AACGAGAGGA AAACACAGAT GGTCATGAGA AGACAGCTTC ATACAGCTTT
    AAAAATTCAT TAAAGATGAG CACAGTGGGC ATCACAGAAA ATGTAAAAGG AGACAACAAG
    AAGTTTGAGA TCTGGTATAA TGGCAGAGAA GAAGTCTATA TCATTCAGGC ATCTTCTGTT
    GAACTGAAAA ACACTTGGAT TTCCGAGATA CGCAAAGTTC TAACAGGACA ACTGGAAGCA
    TGTAGAGAAG CAAGTCAACT ACACCAAAGA ATCACCGAGA GTGTGTACCA TGCACCAATG
    AATTCCTCCA ATATAACCAG GTATAGCAGA AAGTCGTCAA GCATATATGA AAACAAATCC
    GAGACATCAA ACGTGGAAGC ATCTAACAAT AAAAACAGGA ATTCCAGTCC CAGTGCCAGA
    CAAAAGAGAG TTGATGCTTC CACCAGCCTT AACCTTGAGC GCACAGCGCT TGCTAGAAAG
    CGATTCACAT TGCAAGGTCT TTCCAACCGC AGAGCTCCAC CCAAAGATCC AGAATCTAAG
    GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA GCCTCCTCCA TCAGCACTAC AGTTAATGCA
    TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12710
    Clone ID Related Accession (Same CDS sequence) XM_004943438.3 , XM_004943438.2
    Accession Version XM_004943438.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGATCA TCATGCTAAA TTCTGTCTCT GATCTTCATG GCTATATTGA TAAATGTCAA
    CTGACCCGGG AGCTGGGAGG AACCCTGGAG TACGGCCACA GTCAGTGGAT ACATCACCGA
    ACTGCTATTG AAAACTTTGC AATGACAGTG AAAACAACAG CACAGATGCT ACAGACCTTT
    GGAACGGACT TAGCAGAGAC TGAACTTCCC AATGATGTGC AGTGTACAGA GGAGCTCCTA
    TCTGCACACA CTGACCACCA TAGCAAGCTG AAGGATGAGC TGAAACTAGC TGTGAAGCAG
    GGGGCCACAC TACTGACGTG CATTAGGGAG CCAGTCACCC GAAGTGCCAA CAGCAAGCTC
    AGTCCAGATG AACTGGAAAA CGTGGCAACA GTGGAAAGGT TGTTGGCTCA GTTGGATGAA
    ACAGAAAAGG CTTTTGATCA ATTTTGGACC AAGCATCACC TAAAACTAGA ACAATGCTTG
    CAGCTTCGAC ACTTTGAACA TGATTTTAGA GAAGTAAAAT TAACATTGGA TAATCTCATG
    GAAGCACAAG CTGGTTTTTC TGACGTTGGA GACAGTGTGA CTCGAGTAGA ACACCTCCTA
    AGGGAACAGA AGCAACTGGA AGAAAAAGGG CAGGAACCTT TGGAGAAAGC CCAGACTCTA
    GCTCTCCATG GAGAACAGCT CATCCAAAAC AACCATTATG CAGTTGACTC CATTAGACCA
    AAGTGTGTTG AACTCAGGCG TATTTGCGAT GACTTTACTA ATGAGACCAA GAAAAAGTAT
    GATATTTTGG GGAAGTCTCT TGAGCTGCAT AAGCAATTAG ATAAGGCAAG CCAATGGTGT
    GAAGCTGGAA TCTACCTCTT AGCTTCCCAA GCTGTGGACA AGTGCCAGTC ACAAGAGGGA
    GCTGAAACTG CACTCGTTGA AATAGAGAAG TTCCTGGCAA CAGCAAAGGA GCACCAACTC
    TCTAACCCAA AGGAGTTCTA CAATCAGTTT GACACGATTC TCACACCTGA AATAAAGGCC
    AACGCCCAAA GGATTTTACA AAAGCTAGAA GATGTACAAG AGATGTTTGA CAAGAGGCAA
    GTGAGTCTGA AGAAGCTAGC AGCCAAACAG ACCCGTCCAG TGCAACCTGT TGCCCCACAT
    CCTGAATCTT CCCCAAAACG AGCATCACCA AAGACTACCA GACCAGCAGG AGTAGCTACC
    AACAGGAGGT CCGCAGAAAT TACTTGTCCT CCAAAGTCCA TCTCTGAGGC AGAGTTATCA
    AAAAAGAAAA GCATTAAGAA GACTAAAGGT GGAATTAAGA TTGAAGTGAT GCATGAAGCG
    AGCCAAGGGG GATCTAGCAG AGTCTTGGTT ATGAGTGAAA GCGATGAGAA CCTGTCGACG
    AGGAGGAGCC ACATAATCAA TGAGCTGATA GAAACCGAAC GCGTTTATGT GGAAGAACTT
    CAAAGCATAA TAGAGGGATA TGCTTCAGAA ATGGACAATC CAAGTTTATT ACATCTGATT
    CCATCAGTGC TCCAGAACAA AAAGGATATT CTATTTGGGA ACCTCCCAGA AATCTATGAA
    TTCCACAACA GGATTTTCCT TAAAGAGTTA GAAAATTGCA TTGAAAATCC TGAGCTCCTT
    GCCCGATGCT TTTTGAAAAG GAAAGAGGAC CTTCAAATAT ATGAAAAATA CTGTCAGAAC
    AAGCCAAGGT CTGAAGCTCT CTGGAGACAG TGTGGAGACA GCATCTTTTT TCAGGAATGT
    CAGCGTAAAT TGGATCATAA GCTCTCACTA GATGCGTATC TTTTAAAGCC AGTCCAGAGA
    ATCACCAAGT ACCAGCTACT ATTGAAGGAA ATGCTGAAAT GTAGCAAGAA TTCAGAAGGT
    ACTGCTGAAC TGGAAGAGGC CTTGGCCACA GTGCTTGATA TCATCAAATC CGTAAATGAC
    TCCATGCACC AGATAGCAAT CACAGGATAT GAGGGGGACG TAAGTGAGCT TGGTAAGCTG
    TTGATGCAAG GTTCCTTCAA CGTGTGGACT GACCACAAGA AGGGTCACAA CAAGGTGAAG
    GACTTGGCAA GATTCAAGCC GATGCAGAGA CACCTCTTCC TCTACACAAA GATGCTGCTT
    TTTTGCAAGA AACGAGAGGA AAACACAGAT GGTCATGAGA AGACAGCTTC ATACAGCTTT
    AAAAATTCAT TAAAGATGAG CACAGTGGGC ATCACAGAAA ATGTAAAAGG AGACAACAAG
    AAGTTTGAGA TCTGGTATAA TGGCAGAGAA GAAGTCTATA TCATTCAGGC ATCTTCTGTT
    GAACTGAAAA ACACTTGGAT TTCCGAGATA CGCAAAGTTC TAACAGGACA ACTGGAAGCA
    TGTAGAGAAG CAAGTCAACT ACACCAAAGA ATCACCGAGA GTGTGTACCA TGCACCAATG
    AATTCCTCCA ATATAACCAG GTATAGCAGA AAGTCGTCAA GCATATATGA AAACAAATCC
    GAGACATCAA ACGTGGAAGC ATCTAACAAT AAAAACAGGA ATTCCAGTCC CAGTGCCAGA
    CAAAAGAGAG TTGATGCTTC CACCAGCCTT AACCTTGAGC GCACAGCGCT TGCTAGAAAG
    CGATTCACAT TGCAAGGTCT TTCCAACCGC AGAGCTCCAC CCAAAGATCC AGAATCTAAG
    GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA GCCTCCTCCA TCAGCACTAC AGTTAATGCA
    TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943495.1
    CDS157..4008
    Translation

    Target ORF information:

    RefSeq Version XM_004943438.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943438.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGATCA TCATGCTAAA TTCTGTCTCT GATCTTCATG GCTATATTGA TAAATGTCAA
    CTGACCCGGG AGCTGGGAGG AACCCTGGAG TACGGCCACA GTCAGTGGAT ACATCACCGA
    ACTGCTATTG AAAACTTTGC AATGACAGTG AAAACAACAG CACAGATGCT ACAGACCTTT
    GGAACGGACT TAGCAGAGAC TGAACTTCCC AATGATGTGC AGTGTACAGA GGAGCTCCTA
    TCTGCACACA CTGACCACCA TAGCAAGCTG AAGGATGAGC TGAAACTAGC TGTGAAGCAG
    GGGGCCACAC TACTGACGTG CATTAGGGAG CCAGTCACCC GAAGTGCCAA CAGCAAGCTC
    AGTCCAGATG AACTGGAAAA CGTGGCAACA GTGGAAAGGT TGTTGGCTCA GTTGGATGAA
    ACAGAAAAGG CTTTTGATCA ATTTTGGACC AAGCATCACC TAAAACTAGA ACAATGCTTG
    CAGCTTCGAC ACTTTGAACA TGATTTTAGA GAAGTAAAAT TAACATTGGA TAATCTCATG
    GAAGCACAAG CTGGTTTTTC TGACGTTGGA GACAGTGTGA CTCGAGTAGA ACACCTCCTA
    AGGGAACAGA AGCAACTGGA AGAAAAAGGG CAGGAACCTT TGGAGAAAGC CCAGACTCTA
    GCTCTCCATG GAGAACAGCT CATCCAAAAC AACCATTATG CAGTTGACTC CATTAGACCA
    AAGTGTGTTG AACTCAGGCG TATTTGCGAT GACTTTACTA ATGAGACCAA GAAAAAGTAT
    GATATTTTGG GGAAGTCTCT TGAGCTGCAT AAGCAATTAG ATAAGGCAAG CCAATGGTGT
    GAAGCTGGAA TCTACCTCTT AGCTTCCCAA GCTGTGGACA AGTGCCAGTC ACAAGAGGGA
    GCTGAAACTG CACTCGTTGA AATAGAGAAG TTCCTGGCAA CAGCAAAGGA GCACCAACTC
    TCTAACCCAA AGGAGTTCTA CAATCAGTTT GACACGATTC TCACACCTGA AATAAAGGCC
    AACGCCCAAA GGATTTTACA AAAGCTAGAA GATGTACAAG AGATGTTTGA CAAGAGGCAA
    GTGAGTCTGA AGAAGCTAGC AGCCAAACAG ACCCGTCCAG TGCAACCTGT TGCCCCACAT
    CCTGAATCTT CCCCAAAACG AGCATCACCA AAGACTACCA GACCAGCAGG AGTAGCTACC
    AACAGGAGGT CCGCAGAAAT TACTTGTCCT CCAAAGTCCA TCTCTGAGGC AGAGTTATCA
    AAAAAGAAAA GCATTAAGAA GACTAAAGGT GGAATTAAGA TTGAAGTGAT GCATGAAGCG
    AGCCAAGGGG GATCTAGCAG AGTCTTGGTT ATGAGTGAAA GCGATGAGAA CCTGTCGACG
    AGGAGGAGCC ACATAATCAA TGAGCTGATA GAAACCGAAC GCGTTTATGT GGAAGAACTT
    CAAAGCATAA TAGAGGGATA TGCTTCAGAA ATGGACAATC CAAGTTTATT ACATCTGATT
    CCATCAGTGC TCCAGAACAA AAAGGATATT CTATTTGGGA ACCTCCCAGA AATCTATGAA
    TTCCACAACA GGATTTTCCT TAAAGAGTTA GAAAATTGCA TTGAAAATCC TGAGCTCCTT
    GCCCGATGCT TTTTGAAAAG GAAAGAGGAC CTTCAAATAT ATGAAAAATA CTGTCAGAAC
    AAGCCAAGGT CTGAAGCTCT CTGGAGACAG TGTGGAGACA GCATCTTTTT TCAGGAATGT
    CAGCGTAAAT TGGATCATAA GCTCTCACTA GATGCGTATC TTTTAAAGCC AGTCCAGAGA
    ATCACCAAGT ACCAGCTACT ATTGAAGGAA ATGCTGAAAT GTAGCAAGAA TTCAGAAGGT
    ACTGCTGAAC TGGAAGAGGC CTTGGCCACA GTGCTTGATA TCATCAAATC CGTAAATGAC
    TCCATGCACC AGATAGCAAT CACAGGATAT GAGGGGGACG TAAGTGAGCT TGGTAAGCTG
    TTGATGCAAG GTTCCTTCAA CGTGTGGACT GACCACAAGA AGGGTCACAA CAAGGTGAAG
    GACTTGGCAA GATTCAAGCC GATGCAGAGA CACCTCTTCC TCTACACAAA GATGCTGCTT
    TTTTGCAAGA AACGAGAGGA AAACACAGAT GGTCATGAGA AGACAGCTTC ATACAGCTTT
    AAAAATTCAT TAAAGATGAG CACAGTGGGC ATCACAGAAA ATGTAAAAGG AGACAACAAG
    AAGTTTGAGA TCTGGTATAA TGGCAGAGAA GAAGTCTATA TCATTCAGGC ATCTTCTGTT
    GAACTGAAAA ACACTTGGAT TTCCGAGATA CGCAAAGTTC TAACAGGACA ACTGGAAGCA
    TGTAGAGAAG CAAGTCAACT ACACCAAAGA ATCACCGAGA GTGTGTACCA TGCACCAATG
    AATTCCTCCA ATATAACCAG GTATAGCAGA AAGTCGTCAA GCATATATGA AAACAAATCC
    GAGACATCAA ACGTGGAAGC ATCTAACAAT AAAAACAGGA ATTCCAGTCC CAGTGCCAGA
    CAAAAGAGAG TTGATGCTTC CACCAGCCTT AACCTTGAGC GCACAGCGCT TGCTAGAAAG
    CGATTCACAT TGCAAGGTCT TTCCAACCGC AGAGCTCCAC CCAAAGATCC AGAATCTAAG
    GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA GCCTCCTCCA TCAGCACTAC AGTTAATGCA
    TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12711
    Clone ID Related Accession (Same CDS sequence) XM_004943439.3 , XM_004943439.2
    Accession Version XM_004943439.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGTTAAAGTC AAGAGACATG AAATAAAGAG TGACCCAACT
    CCCCTGGGGT TTGAAGATGC TTTTGAGAAC ACCATATTGG CCCAGACTAA ATGTAGCACT
    GGTTCCACCA CAAGACCCGT ACCTAGATCT GCTTCACAGA CAGGTTGGCC CAGTAGGCAA
    ATGCCATCTC TAGACACATT TGAAGACTTT GAAGCCATTC CTTCCAGCAT GGATGAACTC
    TCCAACTCTT CAGACATGGA GGAAGAAACC AACCCTAACA ATGGGACCAA TCTTTTTCGA
    GTAGAAGGCA GTTTTGACAG CTGTTTCCCA GATTCTCTTA CACTTGAAGA TGGAGATCTA
    GTGCAGTTTG TGCAGGAAGG AGAAAATGGG CAATGGTTAG TGAAGAAATT GGTCTCGGAG
    AAAAGTGAAT GGGTTCCTTC CAGTATATTG CAGCCAGCAG AGGGGGACAC TAACAACATA
    ATTAAGAACA GCAATGCAGA CATGTACAAT GATTCCTGCA GCACGGCTTC AGTTGAGTCG
    GACACCAAGG AGAGTGAGGT GGCTAAAAGC TTCCCAGCAG AACAGAGGGC TGGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943496.1
    CDS140..3979
    Translation

    Target ORF information:

    RefSeq Version XM_004943439.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943439.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGTTAAAGTC AAGAGACATG AAATAAAGAG TGACCCAACT
    CCCCTGGGGT TTGAAGATGC TTTTGAGAAC ACCATATTGG CCCAGACTAA ATGTAGCACT
    GGTTCCACCA CAAGACCCGT ACCTAGATCT GCTTCACAGA CAGGTTGGCC CAGTAGGCAA
    ATGCCATCTC TAGACACATT TGAAGACTTT GAAGCCATTC CTTCCAGCAT GGATGAACTC
    TCCAACTCTT CAGACATGGA GGAAGAAACC AACCCTAACA ATGGGACCAA TCTTTTTCGA
    GTAGAAGGCA GTTTTGACAG CTGTTTCCCA GATTCTCTTA CACTTGAAGA TGGAGATCTA
    GTGCAGTTTG TGCAGGAAGG AGAAAATGGG CAATGGTTAG TGAAGAAATT GGTCTCGGAG
    AAAAGTGAAT GGGTTCCTTC CAGTATATTG CAGCCAGCAG AGGGGGACAC TAACAACATA
    ATTAAGAACA GCAATGCAGA CATGTACAAT GATTCCTGCA GCACGGCTTC AGTTGAGTCG
    GACACCAAGG AGAGTGAGGT GGCTAAAAGC TTCCCAGCAG AACAGAGGGC TGGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12711
    Clone ID Related Accession (Same CDS sequence) XM_004943439.3 , XM_004943439.2
    Accession Version XM_004943439.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3840bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGTTAAAGTC AAGAGACATG AAATAAAGAG TGACCCAACT
    CCCCTGGGGT TTGAAGATGC TTTTGAGAAC ACCATATTGG CCCAGACTAA ATGTAGCACT
    GGTTCCACCA CAAGACCCGT ACCTAGATCT GCTTCACAGA CAGGTTGGCC CAGTAGGCAA
    ATGCCATCTC TAGACACATT TGAAGACTTT GAAGCCATTC CTTCCAGCAT GGATGAACTC
    TCCAACTCTT CAGACATGGA GGAAGAAACC AACCCTAACA ATGGGACCAA TCTTTTTCGA
    GTAGAAGGCA GTTTTGACAG CTGTTTCCCA GATTCTCTTA CACTTGAAGA TGGAGATCTA
    GTGCAGTTTG TGCAGGAAGG AGAAAATGGG CAATGGTTAG TGAAGAAATT GGTCTCGGAG
    AAAAGTGAAT GGGTTCCTTC CAGTATATTG CAGCCAGCAG AGGGGGACAC TAACAACATA
    ATTAAGAACA GCAATGCAGA CATGTACAAT GATTCCTGCA GCACGGCTTC AGTTGAGTCG
    GACACCAAGG AGAGTGAGGT GGCTAAAAGC TTCCCAGCAG AACAGAGGGC TGGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943496.1
    CDS157..3996
    Translation

    Target ORF information:

    RefSeq Version XM_004943439.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943439.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGTTGA TGCTTCCACC AGCCTTAACC TTGAGCGCAC AGCGCTTGCT
    AGAAAGCGAT TCACATTGCA AGGTCTTTCC AACCGCAGAG CTCCACCCAA AGATCCAGAA
    TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT TCCCTAGCCT CCTCCATCAG CACTACAGTT
    AATGCATCTG CTGTGACCAA AGTTAAAGTC AAGAGACATG AAATAAAGAG TGACCCAACT
    CCCCTGGGGT TTGAAGATGC TTTTGAGAAC ACCATATTGG CCCAGACTAA ATGTAGCACT
    GGTTCCACCA CAAGACCCGT ACCTAGATCT GCTTCACAGA CAGGTTGGCC CAGTAGGCAA
    ATGCCATCTC TAGACACATT TGAAGACTTT GAAGCCATTC CTTCCAGCAT GGATGAACTC
    TCCAACTCTT CAGACATGGA GGAAGAAACC AACCCTAACA ATGGGACCAA TCTTTTTCGA
    GTAGAAGGCA GTTTTGACAG CTGTTTCCCA GATTCTCTTA CACTTGAAGA TGGAGATCTA
    GTGCAGTTTG TGCAGGAAGG AGAAAATGGG CAATGGTTAG TGAAGAAATT GGTCTCGGAG
    AAAAGTGAAT GGGTTCCTTC CAGTATATTG CAGCCAGCAG AGGGGGACAC TAACAACATA
    ATTAAGAACA GCAATGCAGA CATGTACAAT GATTCCTGCA GCACGGCTTC AGTTGAGTCG
    GACACCAAGG AGAGTGAGGT GGCTAAAAGC TTCCCAGCAG AACAGAGGGC TGGCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12713
    Clone ID Related Accession (Same CDS sequence) XM_004943441.2 , XM_004943441.3
    Accession Version XM_004943441.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGATCC AGAATCTAAG GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA
    GCCTCCTCCA TCAGCACTAC AGTTAATGCA TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT
    GCAGTTAAAG TCAAGAGACA TGAAATAAAG AGTGACCCAA CTCCCCTGGG GTTTGAAGAT
    GCTTTTGAGA ACACCATATT GGCCCAGACT AAATGTAGCA CTGGTTCCAC CACAAGACCC
    GTACCTAGAT CTGCTTCACA GACAGGTTGG CCCAGTAGGC AAATGCCATC TCTAGACACA
    TTTGAAGACT TTGAAGCCAT TCCTTCCAGC ATGGATGAAC TCTCCAACTC TTCAGACATG
    GAGGAAGAAA CCAACCCTAA CAATGGGACC AATCTTTTTC GAGTAGAAGG CAGTTTTGAC
    AGCTGTTTCC CAGATTCTCT TACACTTGAA GATGGAGATC TAGTGCAGTT TGTGCAGGAA
    GGAGAAAATG GGCAATGGTT AGTGAAGAAA TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT
    TCCAGTATAT TGCAGCCAGC AGAGGGGGAC ACTAACAACA TAATTAAGAA CAGCAATGCA
    GACATGTACA ATGATTCCTG CAGCACGGCT TCAGTTGAGT CGGACACCAA GGAGAGTGAG
    GTGGCTAAAA GCTTCCCAGC AGAACAGAGG GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943498.1
    CDS155..3916
    Translation

    Target ORF information:

    RefSeq Version XM_004943441.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943441.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGATCC AGAATCTAAG GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA
    GCCTCCTCCA TCAGCACTAC AGTTAATGCA TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT
    GCAGTTAAAG TCAAGAGACA TGAAATAAAG AGTGACCCAA CTCCCCTGGG GTTTGAAGAT
    GCTTTTGAGA ACACCATATT GGCCCAGACT AAATGTAGCA CTGGTTCCAC CACAAGACCC
    GTACCTAGAT CTGCTTCACA GACAGGTTGG CCCAGTAGGC AAATGCCATC TCTAGACACA
    TTTGAAGACT TTGAAGCCAT TCCTTCCAGC ATGGATGAAC TCTCCAACTC TTCAGACATG
    GAGGAAGAAA CCAACCCTAA CAATGGGACC AATCTTTTTC GAGTAGAAGG CAGTTTTGAC
    AGCTGTTTCC CAGATTCTCT TACACTTGAA GATGGAGATC TAGTGCAGTT TGTGCAGGAA
    GGAGAAAATG GGCAATGGTT AGTGAAGAAA TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT
    TCCAGTATAT TGCAGCCAGC AGAGGGGGAC ACTAACAACA TAATTAAGAA CAGCAATGCA
    GACATGTACA ATGATTCCTG CAGCACGGCT TCAGTTGAGT CGGACACCAA GGAGAGTGAG
    GTGGCTAAAA GCTTCCCAGC AGAACAGAGG GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12713
    Clone ID Related Accession (Same CDS sequence) XM_004943441.2 , XM_004943441.3
    Accession Version XM_004943441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3762bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGATCC AGAATCTAAG GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA
    GCCTCCTCCA TCAGCACTAC AGTTAATGCA TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT
    GCAGTTAAAG TCAAGAGACA TGAAATAAAG AGTGACCCAA CTCCCCTGGG GTTTGAAGAT
    GCTTTTGAGA ACACCATATT GGCCCAGACT AAATGTAGCA CTGGTTCCAC CACAAGACCC
    GTACCTAGAT CTGCTTCACA GACAGGTTGG CCCAGTAGGC AAATGCCATC TCTAGACACA
    TTTGAAGACT TTGAAGCCAT TCCTTCCAGC ATGGATGAAC TCTCCAACTC TTCAGACATG
    GAGGAAGAAA CCAACCCTAA CAATGGGACC AATCTTTTTC GAGTAGAAGG CAGTTTTGAC
    AGCTGTTTCC CAGATTCTCT TACACTTGAA GATGGAGATC TAGTGCAGTT TGTGCAGGAA
    GGAGAAAATG GGCAATGGTT AGTGAAGAAA TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT
    TCCAGTATAT TGCAGCCAGC AGAGGGGGAC ACTAACAACA TAATTAAGAA CAGCAATGCA
    GACATGTACA ATGATTCCTG CAGCACGGCT TCAGTTGAGT CGGACACCAA GGAGAGTGAG
    GTGGCTAAAA GCTTCCCAGC AGAACAGAGG GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943498.1
    CDS139..3900
    Translation

    Target ORF information:

    RefSeq Version XM_004943441.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGATCC AGAATCTAAG GAAAGAGAAG TTGCCCGTCG CTTTTCCCTA
    GCCTCCTCCA TCAGCACTAC AGTTAATGCA TCTGCTGTGA CCAAAGGTCC CCTTGTTCCT
    GCAGTTAAAG TCAAGAGACA TGAAATAAAG AGTGACCCAA CTCCCCTGGG GTTTGAAGAT
    GCTTTTGAGA ACACCATATT GGCCCAGACT AAATGTAGCA CTGGTTCCAC CACAAGACCC
    GTACCTAGAT CTGCTTCACA GACAGGTTGG CCCAGTAGGC AAATGCCATC TCTAGACACA
    TTTGAAGACT TTGAAGCCAT TCCTTCCAGC ATGGATGAAC TCTCCAACTC TTCAGACATG
    GAGGAAGAAA CCAACCCTAA CAATGGGACC AATCTTTTTC GAGTAGAAGG CAGTTTTGAC
    AGCTGTTTCC CAGATTCTCT TACACTTGAA GATGGAGATC TAGTGCAGTT TGTGCAGGAA
    GGAGAAAATG GGCAATGGTT AGTGAAGAAA TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT
    TCCAGTATAT TGCAGCCAGC AGAGGGGGAC ACTAACAACA TAATTAAGAA CAGCAATGCA
    GACATGTACA ATGATTCCTG CAGCACGGCT TCAGTTGAGT CGGACACCAA GGAGAGTGAG
    GTGGCTAAAA GCTTCCCAGC AGAACAGAGG GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12714
    Clone ID Related Accession (Same CDS sequence) XM_004943442.3 , XM_025153437.1 , XM_004943442.2
    Accession Version XM_004943442.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATACGACC GCTGGTTGCA GTGGATATAA TAGAACAGCT ACACAGGCAA 
    TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGAGCGC CAATTATTAC CTTCCCAGAG
    TTTGCAGGAT TCAGTGAAAT ACCCGATGAG GATTTTCTGA ATGTGGTCAC GTACCTAACC
    AGCATTCCCA GTCTGGAGGC TGCCAGCATT GGATTCATCA TCATCATAGA CAGACGACGA
    GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCATT TCCAGGAAAC
    CTACAGCTGG TGTTTGTCCT GCGTCCCTCA CGCTTTATCC AAAGGGCAAT CGCTGACATT
    GGCATTAAAC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GCTAAATTCT
    GTCTCTGATC TTCATGGCTA TATTGATAAA TGTCAACTGA CCCGGGAGCT GGGAGGAACC
    CTGGAGTACG GCCACAGTCA GTGGATACAT CACCGAACTG CTATTGAAAA CTTTGCAATG
    ACAGTGAAAA CAACAGCACA GATGCTACAG ACCTTTGGAA CGGACTTAGC AGAGACTGAA
    CTTCCCAATG ATGTGCAGTG TACAGAGGAG CTCCTATCTG CACACACTGA CCACCATAGC
    AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG AAGCAGGGGG CCACACTACT GACGTGCATT
    AGGGAGCCAG TCACCCGAAG TGCCAACAGC AAGCTCAGTC CAGATGAACT GGAAAACGTG
    GCAACAGTGG AAAGGTTGTT GGCTCAGTTG GATGAAACAG AAAAGGCTTT TGATCAATTT
    TGGACCAAGC ATCACCTAAA ACTAGAACAA TGCTTGCAGC TTCGACACTT TGAACATGAT
    TTTAGAGAAG TAAAATTAAC ATTGGATAAT CTCATGGAAG CACAAGCTGG TTTTTCTGAC
    GTTGGAGACA GTGTGACTCG AGTAGAACAC CTCCTAAGGG AACAGAAGCA ACTGGAAGAA
    AAAGGGCAGG AACCTTTGGA GAAAGCCCAG ACTCTAGCTC TCCATGGAGA ACAGCTCATC
    CAAAACAACC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT
    TGCGATGACT TTACTAATGA GACCAAGAAA AAGTATGATA TTTTGGGGAA GTCTCTTGAG
    CTGCATAAGC AATTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTAGCT
    TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAAATA
    GAGAAGTTCC TGGCAACAGC AAAGGAGCAC CAACTCTCTA ACCCAAAGGA GTTCTACAAT
    CAGTTTGACA CGATTCTCAC ACCTGAAATA AAGGCCAACG CCCAAAGGAT TTTACAAAAG
    CTAGAAGATG TACAAGAGAT GTTTGACAAG AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC
    AAACAGACCC GTCCAGTGCA ACCTGTTGCC CCACATCCTG AATCTTCCCC AAAACGAGCA
    TCACCAAAGA CTACCAGACC AGCAGGAGTA GCTACCAACA GGAGGTCCGC AGAAATTACT
    TGTCCTCCAA AGTCCATCTC TGAGGCAGAG TTATCAAAAA AGAAAAGCAT TAAGAAGACT
    AAAGGTGGAA TTAAGATTGA AGTGATGCAT GAAGCGAGCC AAGGGGGATC TAGCAGAGTC
    TTGGTTATGA GTGAAAGCGA TGAGAACCTG TCGACGAGGA GGAGCCACAT AATCAATGAG
    CTGATAGAAA CCGAACGCGT TTATGTGGAA GAACTTCAAA GCATAATAGA GGGATATGCT
    TCAGAAATGG ACAATCCAAG TTTATTACAT CTGATTCCAT CAGTGCTCCA GAACAAAAAG
    GATATTCTAT TTGGGAACCT CCCAGAAATC TATGAATTCC ACAACAGGAT TTTCCTTAAA
    GAGTTAGAAA ATTGCATTGA AAATCCTGAG CTCCTTGCCC GATGCTTTTT GAAAAGGAAA
    GAGGACCTTC AAATATATGA AAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG
    AGACAGTGTG GAGACAGCAT CTTTTTTCAG GAATGTCAGC GTAAATTGGA TCATAAGCTC
    TCACTAGATG CGTATCTTTT AAAGCCAGTC CAGAGAATCA CCAAGTACCA GCTACTATTG
    AAGGAAATGC TGAAATGTAG CAAGAATTCA GAAGGTACTG CTGAACTGGA AGAGGCCTTG
    GCCACAGTGC TTGATATCAT CAAATCCGTA AATGACTCCA TGCACCAGAT AGCAATCACA
    GGATATGAGG GGGACGTAAG TGAGCTTGGT AAGCTGTTGA TGCAAGGTTC CTTCAACGTG
    TGGACTGACC ACAAGAAGGG TCACAACAAG GTGAAGGACT TGGCAAGATT CAAGCCGATG
    CAGAGACACC TCTTCCTCTA CACAAAGATG CTGCTTTTTT GCAAGAAACG AGAGGAAAAC
    ACAGATGGTC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCATTAAA GATGAGCACA
    GTGGGCATCA CAGAAAATGT AAAAGGAGAC AACAAGAAGT TTGAGATCTG GTATAATGGC
    AGAGAAGAAG TCTATATCAT TCAGGCATCT TCTGTTGAAC TGAAAAACAC TTGGATTTCC
    GAGATACGCA AAGTTCTAAC AGGACAACTG GAAGCATGTA GAGAAGCAAG TCAACTACAC
    CAAAGAATCA CCGAGAGTGT GTACCATGCA CCAATGAATT CCTCCAATAT AACCAGGTAT
    AGCAGAAAGT CGTCAAGCAT ATATGAAAAC AAATCCGAGA CATCAAACGT GGAAGCATCT
    AACAATAAAA ACAGGAATTC CAGTCCCAGT GCCAGACAAA AGAGAGTTGA TGCTTCCACC
    AGCCTTAACC TTGAGCGCAC AGCGCTTGCT AGAAAGCGAT TCACATTGCA AGGTCTTTCC
    AACCGCAGAG CTCCACCCAA AGATCCAGAA TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT
    TCCCTAGCCT CCTCCATCAG CACTACAGTT AATGCATCTG CTGTGACCAA AGGTCCCCTT
    GTTCCTGCAG TTAAAGTCAA GAGACATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT
    GAAGATGCTT TTGAGAACAC CATATTGGCC CAGACTAAAT GTAGCACTGG TTCCACCACA
    AGACCCGTAC CTAGATCTGC TTCACAGACA GGTTGGCCCA GTAGGCAAAT GCCATCTCTA
    GACACATTTG AAGACTTTGA AGCCATTCCT TCCAGCATGG ATGAACTCTC CAACTCTTCA
    GACATGGAGG AAGAAACCAA CCCTAACAAT GGGACCAATC TTTTTCGAGT AGAAGGCAGT
    TTTGACAGCT GTTTCCCAGA TTCTCTTACA CTTGAAGATG GAGATCTAGT GCAGTTTGTG
    CAGGAAGGAG AAAATGGGCA ATGGTTAGTG AAGAAATTGG TCTCGGAGAA AAGTGAATGG
    GTTCCTTCCA GTATATTGCA GCCAGCAGAG GGGGACACTA ACAACATAAT TAAGAACAGC
    AATGCAGACA TGTACAATGA TTCCTGCAGC ACGGCTTCAG TTGAGTCGGA CACCAAGGAG
    AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943499.1
    CDS196..3903
    Translation

    Target ORF information:

    RefSeq Version XM_004943442.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943442.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATACGACC GCTGGTTGCA GTGGATATAA TAGAACAGCT ACACAGGCAA 
    TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGAGCGC CAATTATTAC CTTCCCAGAG
    TTTGCAGGAT TCAGTGAAAT ACCCGATGAG GATTTTCTGA ATGTGGTCAC GTACCTAACC
    AGCATTCCCA GTCTGGAGGC TGCCAGCATT GGATTCATCA TCATCATAGA CAGACGACGA
    GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCATT TCCAGGAAAC
    CTACAGCTGG TGTTTGTCCT GCGTCCCTCA CGCTTTATCC AAAGGGCAAT CGCTGACATT
    GGCATTAAAC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GCTAAATTCT
    GTCTCTGATC TTCATGGCTA TATTGATAAA TGTCAACTGA CCCGGGAGCT GGGAGGAACC
    CTGGAGTACG GCCACAGTCA GTGGATACAT CACCGAACTG CTATTGAAAA CTTTGCAATG
    ACAGTGAAAA CAACAGCACA GATGCTACAG ACCTTTGGAA CGGACTTAGC AGAGACTGAA
    CTTCCCAATG ATGTGCAGTG TACAGAGGAG CTCCTATCTG CACACACTGA CCACCATAGC
    AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG AAGCAGGGGG CCACACTACT GACGTGCATT
    AGGGAGCCAG TCACCCGAAG TGCCAACAGC AAGCTCAGTC CAGATGAACT GGAAAACGTG
    GCAACAGTGG AAAGGTTGTT GGCTCAGTTG GATGAAACAG AAAAGGCTTT TGATCAATTT
    TGGACCAAGC ATCACCTAAA ACTAGAACAA TGCTTGCAGC TTCGACACTT TGAACATGAT
    TTTAGAGAAG TAAAATTAAC ATTGGATAAT CTCATGGAAG CACAAGCTGG TTTTTCTGAC
    GTTGGAGACA GTGTGACTCG AGTAGAACAC CTCCTAAGGG AACAGAAGCA ACTGGAAGAA
    AAAGGGCAGG AACCTTTGGA GAAAGCCCAG ACTCTAGCTC TCCATGGAGA ACAGCTCATC
    CAAAACAACC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT
    TGCGATGACT TTACTAATGA GACCAAGAAA AAGTATGATA TTTTGGGGAA GTCTCTTGAG
    CTGCATAAGC AATTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTAGCT
    TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAAATA
    GAGAAGTTCC TGGCAACAGC AAAGGAGCAC CAACTCTCTA ACCCAAAGGA GTTCTACAAT
    CAGTTTGACA CGATTCTCAC ACCTGAAATA AAGGCCAACG CCCAAAGGAT TTTACAAAAG
    CTAGAAGATG TACAAGAGAT GTTTGACAAG AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC
    AAACAGACCC GTCCAGTGCA ACCTGTTGCC CCACATCCTG AATCTTCCCC AAAACGAGCA
    TCACCAAAGA CTACCAGACC AGCAGGAGTA GCTACCAACA GGAGGTCCGC AGAAATTACT
    TGTCCTCCAA AGTCCATCTC TGAGGCAGAG TTATCAAAAA AGAAAAGCAT TAAGAAGACT
    AAAGGTGGAA TTAAGATTGA AGTGATGCAT GAAGCGAGCC AAGGGGGATC TAGCAGAGTC
    TTGGTTATGA GTGAAAGCGA TGAGAACCTG TCGACGAGGA GGAGCCACAT AATCAATGAG
    CTGATAGAAA CCGAACGCGT TTATGTGGAA GAACTTCAAA GCATAATAGA GGGATATGCT
    TCAGAAATGG ACAATCCAAG TTTATTACAT CTGATTCCAT CAGTGCTCCA GAACAAAAAG
    GATATTCTAT TTGGGAACCT CCCAGAAATC TATGAATTCC ACAACAGGAT TTTCCTTAAA
    GAGTTAGAAA ATTGCATTGA AAATCCTGAG CTCCTTGCCC GATGCTTTTT GAAAAGGAAA
    GAGGACCTTC AAATATATGA AAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG
    AGACAGTGTG GAGACAGCAT CTTTTTTCAG GAATGTCAGC GTAAATTGGA TCATAAGCTC
    TCACTAGATG CGTATCTTTT AAAGCCAGTC CAGAGAATCA CCAAGTACCA GCTACTATTG
    AAGGAAATGC TGAAATGTAG CAAGAATTCA GAAGGTACTG CTGAACTGGA AGAGGCCTTG
    GCCACAGTGC TTGATATCAT CAAATCCGTA AATGACTCCA TGCACCAGAT AGCAATCACA
    GGATATGAGG GGGACGTAAG TGAGCTTGGT AAGCTGTTGA TGCAAGGTTC CTTCAACGTG
    TGGACTGACC ACAAGAAGGG TCACAACAAG GTGAAGGACT TGGCAAGATT CAAGCCGATG
    CAGAGACACC TCTTCCTCTA CACAAAGATG CTGCTTTTTT GCAAGAAACG AGAGGAAAAC
    ACAGATGGTC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCATTAAA GATGAGCACA
    GTGGGCATCA CAGAAAATGT AAAAGGAGAC AACAAGAAGT TTGAGATCTG GTATAATGGC
    AGAGAAGAAG TCTATATCAT TCAGGCATCT TCTGTTGAAC TGAAAAACAC TTGGATTTCC
    GAGATACGCA AAGTTCTAAC AGGACAACTG GAAGCATGTA GAGAAGCAAG TCAACTACAC
    CAAAGAATCA CCGAGAGTGT GTACCATGCA CCAATGAATT CCTCCAATAT AACCAGGTAT
    AGCAGAAAGT CGTCAAGCAT ATATGAAAAC AAATCCGAGA CATCAAACGT GGAAGCATCT
    AACAATAAAA ACAGGAATTC CAGTCCCAGT GCCAGACAAA AGAGAGTTGA TGCTTCCACC
    AGCCTTAACC TTGAGCGCAC AGCGCTTGCT AGAAAGCGAT TCACATTGCA AGGTCTTTCC
    AACCGCAGAG CTCCACCCAA AGATCCAGAA TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT
    TCCCTAGCCT CCTCCATCAG CACTACAGTT AATGCATCTG CTGTGACCAA AGGTCCCCTT
    GTTCCTGCAG TTAAAGTCAA GAGACATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT
    GAAGATGCTT TTGAGAACAC CATATTGGCC CAGACTAAAT GTAGCACTGG TTCCACCACA
    AGACCCGTAC CTAGATCTGC TTCACAGACA GGTTGGCCCA GTAGGCAAAT GCCATCTCTA
    GACACATTTG AAGACTTTGA AGCCATTCCT TCCAGCATGG ATGAACTCTC CAACTCTTCA
    GACATGGAGG AAGAAACCAA CCCTAACAAT GGGACCAATC TTTTTCGAGT AGAAGGCAGT
    TTTGACAGCT GTTTCCCAGA TTCTCTTACA CTTGAAGATG GAGATCTAGT GCAGTTTGTG
    CAGGAAGGAG AAAATGGGCA ATGGTTAGTG AAGAAATTGG TCTCGGAGAA AAGTGAATGG
    GTTCCTTCCA GTATATTGCA GCCAGCAGAG GGGGACACTA ACAACATAAT TAAGAACAGC
    AATGCAGACA TGTACAATGA TTCCTGCAGC ACGGCTTCAG TTGAGTCGGA CACCAAGGAG
    AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12714
    Clone ID Related Accession (Same CDS sequence) XM_004943442.3 , XM_025153437.1 , XM_004943442.2
    Accession Version XM_025153437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATACGACC GCTGGTTGCA GTGGATATAA TAGAACAGCT ACACAGGCAA 
    TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGAGCGC CAATTATTAC CTTCCCAGAG
    TTTGCAGGAT TCAGTGAAAT ACCCGATGAG GATTTTCTGA ATGTGGTCAC GTACCTAACC
    AGCATTCCCA GTCTGGAGGC TGCCAGCATT GGATTCATCA TCATCATAGA CAGACGACGA
    GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCATT TCCAGGAAAC
    CTACAGCTGG TGTTTGTCCT GCGTCCCTCA CGCTTTATCC AAAGGGCAAT CGCTGACATT
    GGCATTAAAC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GCTAAATTCT
    GTCTCTGATC TTCATGGCTA TATTGATAAA TGTCAACTGA CCCGGGAGCT GGGAGGAACC
    CTGGAGTACG GCCACAGTCA GTGGATACAT CACCGAACTG CTATTGAAAA CTTTGCAATG
    ACAGTGAAAA CAACAGCACA GATGCTACAG ACCTTTGGAA CGGACTTAGC AGAGACTGAA
    CTTCCCAATG ATGTGCAGTG TACAGAGGAG CTCCTATCTG CACACACTGA CCACCATAGC
    AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG AAGCAGGGGG CCACACTACT GACGTGCATT
    AGGGAGCCAG TCACCCGAAG TGCCAACAGC AAGCTCAGTC CAGATGAACT GGAAAACGTG
    GCAACAGTGG AAAGGTTGTT GGCTCAGTTG GATGAAACAG AAAAGGCTTT TGATCAATTT
    TGGACCAAGC ATCACCTAAA ACTAGAACAA TGCTTGCAGC TTCGACACTT TGAACATGAT
    TTTAGAGAAG TAAAATTAAC ATTGGATAAT CTCATGGAAG CACAAGCTGG TTTTTCTGAC
    GTTGGAGACA GTGTGACTCG AGTAGAACAC CTCCTAAGGG AACAGAAGCA ACTGGAAGAA
    AAAGGGCAGG AACCTTTGGA GAAAGCCCAG ACTCTAGCTC TCCATGGAGA ACAGCTCATC
    CAAAACAACC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT
    TGCGATGACT TTACTAATGA GACCAAGAAA AAGTATGATA TTTTGGGGAA GTCTCTTGAG
    CTGCATAAGC AATTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTAGCT
    TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAAATA
    GAGAAGTTCC TGGCAACAGC AAAGGAGCAC CAACTCTCTA ACCCAAAGGA GTTCTACAAT
    CAGTTTGACA CGATTCTCAC ACCTGAAATA AAGGCCAACG CCCAAAGGAT TTTACAAAAG
    CTAGAAGATG TACAAGAGAT GTTTGACAAG AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC
    AAACAGACCC GTCCAGTGCA ACCTGTTGCC CCACATCCTG AATCTTCCCC AAAACGAGCA
    TCACCAAAGA CTACCAGACC AGCAGGAGTA GCTACCAACA GGAGGTCCGC AGAAATTACT
    TGTCCTCCAA AGTCCATCTC TGAGGCAGAG TTATCAAAAA AGAAAAGCAT TAAGAAGACT
    AAAGGTGGAA TTAAGATTGA AGTGATGCAT GAAGCGAGCC AAGGGGGATC TAGCAGAGTC
    TTGGTTATGA GTGAAAGCGA TGAGAACCTG TCGACGAGGA GGAGCCACAT AATCAATGAG
    CTGATAGAAA CCGAACGCGT TTATGTGGAA GAACTTCAAA GCATAATAGA GGGATATGCT
    TCAGAAATGG ACAATCCAAG TTTATTACAT CTGATTCCAT CAGTGCTCCA GAACAAAAAG
    GATATTCTAT TTGGGAACCT CCCAGAAATC TATGAATTCC ACAACAGGAT TTTCCTTAAA
    GAGTTAGAAA ATTGCATTGA AAATCCTGAG CTCCTTGCCC GATGCTTTTT GAAAAGGAAA
    GAGGACCTTC AAATATATGA AAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG
    AGACAGTGTG GAGACAGCAT CTTTTTTCAG GAATGTCAGC GTAAATTGGA TCATAAGCTC
    TCACTAGATG CGTATCTTTT AAAGCCAGTC CAGAGAATCA CCAAGTACCA GCTACTATTG
    AAGGAAATGC TGAAATGTAG CAAGAATTCA GAAGGTACTG CTGAACTGGA AGAGGCCTTG
    GCCACAGTGC TTGATATCAT CAAATCCGTA AATGACTCCA TGCACCAGAT AGCAATCACA
    GGATATGAGG GGGACGTAAG TGAGCTTGGT AAGCTGTTGA TGCAAGGTTC CTTCAACGTG
    TGGACTGACC ACAAGAAGGG TCACAACAAG GTGAAGGACT TGGCAAGATT CAAGCCGATG
    CAGAGACACC TCTTCCTCTA CACAAAGATG CTGCTTTTTT GCAAGAAACG AGAGGAAAAC
    ACAGATGGTC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCATTAAA GATGAGCACA
    GTGGGCATCA CAGAAAATGT AAAAGGAGAC AACAAGAAGT TTGAGATCTG GTATAATGGC
    AGAGAAGAAG TCTATATCAT TCAGGCATCT TCTGTTGAAC TGAAAAACAC TTGGATTTCC
    GAGATACGCA AAGTTCTAAC AGGACAACTG GAAGCATGTA GAGAAGCAAG TCAACTACAC
    CAAAGAATCA CCGAGAGTGT GTACCATGCA CCAATGAATT CCTCCAATAT AACCAGGTAT
    AGCAGAAAGT CGTCAAGCAT ATATGAAAAC AAATCCGAGA CATCAAACGT GGAAGCATCT
    AACAATAAAA ACAGGAATTC CAGTCCCAGT GCCAGACAAA AGAGAGTTGA TGCTTCCACC
    AGCCTTAACC TTGAGCGCAC AGCGCTTGCT AGAAAGCGAT TCACATTGCA AGGTCTTTCC
    AACCGCAGAG CTCCACCCAA AGATCCAGAA TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT
    TCCCTAGCCT CCTCCATCAG CACTACAGTT AATGCATCTG CTGTGACCAA AGGTCCCCTT
    GTTCCTGCAG TTAAAGTCAA GAGACATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT
    GAAGATGCTT TTGAGAACAC CATATTGGCC CAGACTAAAT GTAGCACTGG TTCCACCACA
    AGACCCGTAC CTAGATCTGC TTCACAGACA GGTTGGCCCA GTAGGCAAAT GCCATCTCTA
    GACACATTTG AAGACTTTGA AGCCATTCCT TCCAGCATGG ATGAACTCTC CAACTCTTCA
    GACATGGAGG AAGAAACCAA CCCTAACAAT GGGACCAATC TTTTTCGAGT AGAAGGCAGT
    TTTGACAGCT GTTTCCCAGA TTCTCTTACA CTTGAAGATG GAGATCTAGT GCAGTTTGTG
    CAGGAAGGAG AAAATGGGCA ATGGTTAGTG AAGAAATTGG TCTCGGAGAA AAGTGAATGG
    GTTCCTTCCA GTATATTGCA GCCAGCAGAG GGGGACACTA ACAACATAAT TAAGAACAGC
    AATGCAGACA TGTACAATGA TTCCTGCAGC ACGGCTTCAG TTGAGTCGGA CACCAAGGAG
    AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025009205.1
    CDS28..3735
    Translation

    Target ORF information:

    RefSeq Version XM_025153437.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025153437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATACGACC GCTGGTTGCA GTGGATATAA TAGAACAGCT ACACAGGCAA 
    TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGAGCGC CAATTATTAC CTTCCCAGAG
    TTTGCAGGAT TCAGTGAAAT ACCCGATGAG GATTTTCTGA ATGTGGTCAC GTACCTAACC
    AGCATTCCCA GTCTGGAGGC TGCCAGCATT GGATTCATCA TCATCATAGA CAGACGACGA
    GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCATT TCCAGGAAAC
    CTACAGCTGG TGTTTGTCCT GCGTCCCTCA CGCTTTATCC AAAGGGCAAT CGCTGACATT
    GGCATTAAAC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GCTAAATTCT
    GTCTCTGATC TTCATGGCTA TATTGATAAA TGTCAACTGA CCCGGGAGCT GGGAGGAACC
    CTGGAGTACG GCCACAGTCA GTGGATACAT CACCGAACTG CTATTGAAAA CTTTGCAATG
    ACAGTGAAAA CAACAGCACA GATGCTACAG ACCTTTGGAA CGGACTTAGC AGAGACTGAA
    CTTCCCAATG ATGTGCAGTG TACAGAGGAG CTCCTATCTG CACACACTGA CCACCATAGC
    AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG AAGCAGGGGG CCACACTACT GACGTGCATT
    AGGGAGCCAG TCACCCGAAG TGCCAACAGC AAGCTCAGTC CAGATGAACT GGAAAACGTG
    GCAACAGTGG AAAGGTTGTT GGCTCAGTTG GATGAAACAG AAAAGGCTTT TGATCAATTT
    TGGACCAAGC ATCACCTAAA ACTAGAACAA TGCTTGCAGC TTCGACACTT TGAACATGAT
    TTTAGAGAAG TAAAATTAAC ATTGGATAAT CTCATGGAAG CACAAGCTGG TTTTTCTGAC
    GTTGGAGACA GTGTGACTCG AGTAGAACAC CTCCTAAGGG AACAGAAGCA ACTGGAAGAA
    AAAGGGCAGG AACCTTTGGA GAAAGCCCAG ACTCTAGCTC TCCATGGAGA ACAGCTCATC
    CAAAACAACC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT
    TGCGATGACT TTACTAATGA GACCAAGAAA AAGTATGATA TTTTGGGGAA GTCTCTTGAG
    CTGCATAAGC AATTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTAGCT
    TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAAATA
    GAGAAGTTCC TGGCAACAGC AAAGGAGCAC CAACTCTCTA ACCCAAAGGA GTTCTACAAT
    CAGTTTGACA CGATTCTCAC ACCTGAAATA AAGGCCAACG CCCAAAGGAT TTTACAAAAG
    CTAGAAGATG TACAAGAGAT GTTTGACAAG AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC
    AAACAGACCC GTCCAGTGCA ACCTGTTGCC CCACATCCTG AATCTTCCCC AAAACGAGCA
    TCACCAAAGA CTACCAGACC AGCAGGAGTA GCTACCAACA GGAGGTCCGC AGAAATTACT
    TGTCCTCCAA AGTCCATCTC TGAGGCAGAG TTATCAAAAA AGAAAAGCAT TAAGAAGACT
    AAAGGTGGAA TTAAGATTGA AGTGATGCAT GAAGCGAGCC AAGGGGGATC TAGCAGAGTC
    TTGGTTATGA GTGAAAGCGA TGAGAACCTG TCGACGAGGA GGAGCCACAT AATCAATGAG
    CTGATAGAAA CCGAACGCGT TTATGTGGAA GAACTTCAAA GCATAATAGA GGGATATGCT
    TCAGAAATGG ACAATCCAAG TTTATTACAT CTGATTCCAT CAGTGCTCCA GAACAAAAAG
    GATATTCTAT TTGGGAACCT CCCAGAAATC TATGAATTCC ACAACAGGAT TTTCCTTAAA
    GAGTTAGAAA ATTGCATTGA AAATCCTGAG CTCCTTGCCC GATGCTTTTT GAAAAGGAAA
    GAGGACCTTC AAATATATGA AAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG
    AGACAGTGTG GAGACAGCAT CTTTTTTCAG GAATGTCAGC GTAAATTGGA TCATAAGCTC
    TCACTAGATG CGTATCTTTT AAAGCCAGTC CAGAGAATCA CCAAGTACCA GCTACTATTG
    AAGGAAATGC TGAAATGTAG CAAGAATTCA GAAGGTACTG CTGAACTGGA AGAGGCCTTG
    GCCACAGTGC TTGATATCAT CAAATCCGTA AATGACTCCA TGCACCAGAT AGCAATCACA
    GGATATGAGG GGGACGTAAG TGAGCTTGGT AAGCTGTTGA TGCAAGGTTC CTTCAACGTG
    TGGACTGACC ACAAGAAGGG TCACAACAAG GTGAAGGACT TGGCAAGATT CAAGCCGATG
    CAGAGACACC TCTTCCTCTA CACAAAGATG CTGCTTTTTT GCAAGAAACG AGAGGAAAAC
    ACAGATGGTC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCATTAAA GATGAGCACA
    GTGGGCATCA CAGAAAATGT AAAAGGAGAC AACAAGAAGT TTGAGATCTG GTATAATGGC
    AGAGAAGAAG TCTATATCAT TCAGGCATCT TCTGTTGAAC TGAAAAACAC TTGGATTTCC
    GAGATACGCA AAGTTCTAAC AGGACAACTG GAAGCATGTA GAGAAGCAAG TCAACTACAC
    CAAAGAATCA CCGAGAGTGT GTACCATGCA CCAATGAATT CCTCCAATAT AACCAGGTAT
    AGCAGAAAGT CGTCAAGCAT ATATGAAAAC AAATCCGAGA CATCAAACGT GGAAGCATCT
    AACAATAAAA ACAGGAATTC CAGTCCCAGT GCCAGACAAA AGAGAGTTGA TGCTTCCACC
    AGCCTTAACC TTGAGCGCAC AGCGCTTGCT AGAAAGCGAT TCACATTGCA AGGTCTTTCC
    AACCGCAGAG CTCCACCCAA AGATCCAGAA TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT
    TCCCTAGCCT CCTCCATCAG CACTACAGTT AATGCATCTG CTGTGACCAA AGGTCCCCTT
    GTTCCTGCAG TTAAAGTCAA GAGACATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT
    GAAGATGCTT TTGAGAACAC CATATTGGCC CAGACTAAAT GTAGCACTGG TTCCACCACA
    AGACCCGTAC CTAGATCTGC TTCACAGACA GGTTGGCCCA GTAGGCAAAT GCCATCTCTA
    GACACATTTG AAGACTTTGA AGCCATTCCT TCCAGCATGG ATGAACTCTC CAACTCTTCA
    GACATGGAGG AAGAAACCAA CCCTAACAAT GGGACCAATC TTTTTCGAGT AGAAGGCAGT
    TTTGACAGCT GTTTCCCAGA TTCTCTTACA CTTGAAGATG GAGATCTAGT GCAGTTTGTG
    CAGGAAGGAG AAAATGGGCA ATGGTTAGTG AAGAAATTGG TCTCGGAGAA AAGTGAATGG
    GTTCCTTCCA GTATATTGCA GCCAGCAGAG GGGGACACTA ACAACATAAT TAAGAACAGC
    AATGCAGACA TGTACAATGA TTCCTGCAGC ACGGCTTCAG TTGAGTCGGA CACCAAGGAG
    AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12714
    Clone ID Related Accession (Same CDS sequence) XM_004943442.3 , XM_025153437.1 , XM_004943442.2
    Accession Version XM_004943442.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3708bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATACGACC GCTGGTTGCA GTGGATATAA TAGAACAGCT ACACAGGCAA 
    TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGAGCGC CAATTATTAC CTTCCCAGAG
    TTTGCAGGAT TCAGTGAAAT ACCCGATGAG GATTTTCTGA ATGTGGTCAC GTACCTAACC
    AGCATTCCCA GTCTGGAGGC TGCCAGCATT GGATTCATCA TCATCATAGA CAGACGACGA
    GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCATT TCCAGGAAAC
    CTACAGCTGG TGTTTGTCCT GCGTCCCTCA CGCTTTATCC AAAGGGCAAT CGCTGACATT
    GGCATTAAAC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GCTAAATTCT
    GTCTCTGATC TTCATGGCTA TATTGATAAA TGTCAACTGA CCCGGGAGCT GGGAGGAACC
    CTGGAGTACG GCCACAGTCA GTGGATACAT CACCGAACTG CTATTGAAAA CTTTGCAATG
    ACAGTGAAAA CAACAGCACA GATGCTACAG ACCTTTGGAA CGGACTTAGC AGAGACTGAA
    CTTCCCAATG ATGTGCAGTG TACAGAGGAG CTCCTATCTG CACACACTGA CCACCATAGC
    AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG AAGCAGGGGG CCACACTACT GACGTGCATT
    AGGGAGCCAG TCACCCGAAG TGCCAACAGC AAGCTCAGTC CAGATGAACT GGAAAACGTG
    GCAACAGTGG AAAGGTTGTT GGCTCAGTTG GATGAAACAG AAAAGGCTTT TGATCAATTT
    TGGACCAAGC ATCACCTAAA ACTAGAACAA TGCTTGCAGC TTCGACACTT TGAACATGAT
    TTTAGAGAAG TAAAATTAAC ATTGGATAAT CTCATGGAAG CACAAGCTGG TTTTTCTGAC
    GTTGGAGACA GTGTGACTCG AGTAGAACAC CTCCTAAGGG AACAGAAGCA ACTGGAAGAA
    AAAGGGCAGG AACCTTTGGA GAAAGCCCAG ACTCTAGCTC TCCATGGAGA ACAGCTCATC
    CAAAACAACC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT
    TGCGATGACT TTACTAATGA GACCAAGAAA AAGTATGATA TTTTGGGGAA GTCTCTTGAG
    CTGCATAAGC AATTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTAGCT
    TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAAATA
    GAGAAGTTCC TGGCAACAGC AAAGGAGCAC CAACTCTCTA ACCCAAAGGA GTTCTACAAT
    CAGTTTGACA CGATTCTCAC ACCTGAAATA AAGGCCAACG CCCAAAGGAT TTTACAAAAG
    CTAGAAGATG TACAAGAGAT GTTTGACAAG AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC
    AAACAGACCC GTCCAGTGCA ACCTGTTGCC CCACATCCTG AATCTTCCCC AAAACGAGCA
    TCACCAAAGA CTACCAGACC AGCAGGAGTA GCTACCAACA GGAGGTCCGC AGAAATTACT
    TGTCCTCCAA AGTCCATCTC TGAGGCAGAG TTATCAAAAA AGAAAAGCAT TAAGAAGACT
    AAAGGTGGAA TTAAGATTGA AGTGATGCAT GAAGCGAGCC AAGGGGGATC TAGCAGAGTC
    TTGGTTATGA GTGAAAGCGA TGAGAACCTG TCGACGAGGA GGAGCCACAT AATCAATGAG
    CTGATAGAAA CCGAACGCGT TTATGTGGAA GAACTTCAAA GCATAATAGA GGGATATGCT
    TCAGAAATGG ACAATCCAAG TTTATTACAT CTGATTCCAT CAGTGCTCCA GAACAAAAAG
    GATATTCTAT TTGGGAACCT CCCAGAAATC TATGAATTCC ACAACAGGAT TTTCCTTAAA
    GAGTTAGAAA ATTGCATTGA AAATCCTGAG CTCCTTGCCC GATGCTTTTT GAAAAGGAAA
    GAGGACCTTC AAATATATGA AAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG
    AGACAGTGTG GAGACAGCAT CTTTTTTCAG GAATGTCAGC GTAAATTGGA TCATAAGCTC
    TCACTAGATG CGTATCTTTT AAAGCCAGTC CAGAGAATCA CCAAGTACCA GCTACTATTG
    AAGGAAATGC TGAAATGTAG CAAGAATTCA GAAGGTACTG CTGAACTGGA AGAGGCCTTG
    GCCACAGTGC TTGATATCAT CAAATCCGTA AATGACTCCA TGCACCAGAT AGCAATCACA
    GGATATGAGG GGGACGTAAG TGAGCTTGGT AAGCTGTTGA TGCAAGGTTC CTTCAACGTG
    TGGACTGACC ACAAGAAGGG TCACAACAAG GTGAAGGACT TGGCAAGATT CAAGCCGATG
    CAGAGACACC TCTTCCTCTA CACAAAGATG CTGCTTTTTT GCAAGAAACG AGAGGAAAAC
    ACAGATGGTC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCATTAAA GATGAGCACA
    GTGGGCATCA CAGAAAATGT AAAAGGAGAC AACAAGAAGT TTGAGATCTG GTATAATGGC
    AGAGAAGAAG TCTATATCAT TCAGGCATCT TCTGTTGAAC TGAAAAACAC TTGGATTTCC
    GAGATACGCA AAGTTCTAAC AGGACAACTG GAAGCATGTA GAGAAGCAAG TCAACTACAC
    CAAAGAATCA CCGAGAGTGT GTACCATGCA CCAATGAATT CCTCCAATAT AACCAGGTAT
    AGCAGAAAGT CGTCAAGCAT ATATGAAAAC AAATCCGAGA CATCAAACGT GGAAGCATCT
    AACAATAAAA ACAGGAATTC CAGTCCCAGT GCCAGACAAA AGAGAGTTGA TGCTTCCACC
    AGCCTTAACC TTGAGCGCAC AGCGCTTGCT AGAAAGCGAT TCACATTGCA AGGTCTTTCC
    AACCGCAGAG CTCCACCCAA AGATCCAGAA TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT
    TCCCTAGCCT CCTCCATCAG CACTACAGTT AATGCATCTG CTGTGACCAA AGGTCCCCTT
    GTTCCTGCAG TTAAAGTCAA GAGACATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT
    GAAGATGCTT TTGAGAACAC CATATTGGCC CAGACTAAAT GTAGCACTGG TTCCACCACA
    AGACCCGTAC CTAGATCTGC TTCACAGACA GGTTGGCCCA GTAGGCAAAT GCCATCTCTA
    GACACATTTG AAGACTTTGA AGCCATTCCT TCCAGCATGG ATGAACTCTC CAACTCTTCA
    GACATGGAGG AAGAAACCAA CCCTAACAAT GGGACCAATC TTTTTCGAGT AGAAGGCAGT
    TTTGACAGCT GTTTCCCAGA TTCTCTTACA CTTGAAGATG GAGATCTAGT GCAGTTTGTG
    CAGGAAGGAG AAAATGGGCA ATGGTTAGTG AAGAAATTGG TCTCGGAGAA AAGTGAATGG
    GTTCCTTCCA GTATATTGCA GCCAGCAGAG GGGGACACTA ACAACATAAT TAAGAACAGC
    AATGCAGACA TGTACAATGA TTCCTGCAGC ACGGCTTCAG TTGAGTCGGA CACCAAGGAG
    AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943499.1
    CDS208..3915
    Translation

    Target ORF information:

    RefSeq Version XM_004943442.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943442.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATACGACC GCTGGTTGCA GTGGATATAA TAGAACAGCT ACACAGGCAA 
    TTTGCTATCT TGTCAGGAGG AAGAGGGAAG GATGGAGCGC CAATTATTAC CTTCCCAGAG
    TTTGCAGGAT TCAGTGAAAT ACCCGATGAG GATTTTCTGA ATGTGGTCAC GTACCTAACC
    AGCATTCCCA GTCTGGAGGC TGCCAGCATT GGATTCATCA TCATCATAGA CAGACGACGA
    GACAAATGGA GCGCAGTCAA GGCATCCCTG ACACGGATAG CAGGAGCATT TCCAGGAAAC
    CTACAGCTGG TGTTTGTCCT GCGTCCCTCA CGCTTTATCC AAAGGGCAAT CGCTGACATT
    GGCATTAAAC TCTATCGAGA TGACTTTAAA ATGAAGGTAC CGATCATCAT GCTAAATTCT
    GTCTCTGATC TTCATGGCTA TATTGATAAA TGTCAACTGA CCCGGGAGCT GGGAGGAACC
    CTGGAGTACG GCCACAGTCA GTGGATACAT CACCGAACTG CTATTGAAAA CTTTGCAATG
    ACAGTGAAAA CAACAGCACA GATGCTACAG ACCTTTGGAA CGGACTTAGC AGAGACTGAA
    CTTCCCAATG ATGTGCAGTG TACAGAGGAG CTCCTATCTG CACACACTGA CCACCATAGC
    AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG AAGCAGGGGG CCACACTACT GACGTGCATT
    AGGGAGCCAG TCACCCGAAG TGCCAACAGC AAGCTCAGTC CAGATGAACT GGAAAACGTG
    GCAACAGTGG AAAGGTTGTT GGCTCAGTTG GATGAAACAG AAAAGGCTTT TGATCAATTT
    TGGACCAAGC ATCACCTAAA ACTAGAACAA TGCTTGCAGC TTCGACACTT TGAACATGAT
    TTTAGAGAAG TAAAATTAAC ATTGGATAAT CTCATGGAAG CACAAGCTGG TTTTTCTGAC
    GTTGGAGACA GTGTGACTCG AGTAGAACAC CTCCTAAGGG AACAGAAGCA ACTGGAAGAA
    AAAGGGCAGG AACCTTTGGA GAAAGCCCAG ACTCTAGCTC TCCATGGAGA ACAGCTCATC
    CAAAACAACC ATTATGCAGT TGACTCCATT AGACCAAAGT GTGTTGAACT CAGGCGTATT
    TGCGATGACT TTACTAATGA GACCAAGAAA AAGTATGATA TTTTGGGGAA GTCTCTTGAG
    CTGCATAAGC AATTAGATAA GGCAAGCCAA TGGTGTGAAG CTGGAATCTA CCTCTTAGCT
    TCCCAAGCTG TGGACAAGTG CCAGTCACAA GAGGGAGCTG AAACTGCACT CGTTGAAATA
    GAGAAGTTCC TGGCAACAGC AAAGGAGCAC CAACTCTCTA ACCCAAAGGA GTTCTACAAT
    CAGTTTGACA CGATTCTCAC ACCTGAAATA AAGGCCAACG CCCAAAGGAT TTTACAAAAG
    CTAGAAGATG TACAAGAGAT GTTTGACAAG AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC
    AAACAGACCC GTCCAGTGCA ACCTGTTGCC CCACATCCTG AATCTTCCCC AAAACGAGCA
    TCACCAAAGA CTACCAGACC AGCAGGAGTA GCTACCAACA GGAGGTCCGC AGAAATTACT
    TGTCCTCCAA AGTCCATCTC TGAGGCAGAG TTATCAAAAA AGAAAAGCAT TAAGAAGACT
    AAAGGTGGAA TTAAGATTGA AGTGATGCAT GAAGCGAGCC AAGGGGGATC TAGCAGAGTC
    TTGGTTATGA GTGAAAGCGA TGAGAACCTG TCGACGAGGA GGAGCCACAT AATCAATGAG
    CTGATAGAAA CCGAACGCGT TTATGTGGAA GAACTTCAAA GCATAATAGA GGGATATGCT
    TCAGAAATGG ACAATCCAAG TTTATTACAT CTGATTCCAT CAGTGCTCCA GAACAAAAAG
    GATATTCTAT TTGGGAACCT CCCAGAAATC TATGAATTCC ACAACAGGAT TTTCCTTAAA
    GAGTTAGAAA ATTGCATTGA AAATCCTGAG CTCCTTGCCC GATGCTTTTT GAAAAGGAAA
    GAGGACCTTC AAATATATGA AAAATACTGT CAGAACAAGC CAAGGTCTGA AGCTCTCTGG
    AGACAGTGTG GAGACAGCAT CTTTTTTCAG GAATGTCAGC GTAAATTGGA TCATAAGCTC
    TCACTAGATG CGTATCTTTT AAAGCCAGTC CAGAGAATCA CCAAGTACCA GCTACTATTG
    AAGGAAATGC TGAAATGTAG CAAGAATTCA GAAGGTACTG CTGAACTGGA AGAGGCCTTG
    GCCACAGTGC TTGATATCAT CAAATCCGTA AATGACTCCA TGCACCAGAT AGCAATCACA
    GGATATGAGG GGGACGTAAG TGAGCTTGGT AAGCTGTTGA TGCAAGGTTC CTTCAACGTG
    TGGACTGACC ACAAGAAGGG TCACAACAAG GTGAAGGACT TGGCAAGATT CAAGCCGATG
    CAGAGACACC TCTTCCTCTA CACAAAGATG CTGCTTTTTT GCAAGAAACG AGAGGAAAAC
    ACAGATGGTC ATGAGAAGAC AGCTTCATAC AGCTTTAAAA ATTCATTAAA GATGAGCACA
    GTGGGCATCA CAGAAAATGT AAAAGGAGAC AACAAGAAGT TTGAGATCTG GTATAATGGC
    AGAGAAGAAG TCTATATCAT TCAGGCATCT TCTGTTGAAC TGAAAAACAC TTGGATTTCC
    GAGATACGCA AAGTTCTAAC AGGACAACTG GAAGCATGTA GAGAAGCAAG TCAACTACAC
    CAAAGAATCA CCGAGAGTGT GTACCATGCA CCAATGAATT CCTCCAATAT AACCAGGTAT
    AGCAGAAAGT CGTCAAGCAT ATATGAAAAC AAATCCGAGA CATCAAACGT GGAAGCATCT
    AACAATAAAA ACAGGAATTC CAGTCCCAGT GCCAGACAAA AGAGAGTTGA TGCTTCCACC
    AGCCTTAACC TTGAGCGCAC AGCGCTTGCT AGAAAGCGAT TCACATTGCA AGGTCTTTCC
    AACCGCAGAG CTCCACCCAA AGATCCAGAA TCTAAGGAAA GAGAAGTTGC CCGTCGCTTT
    TCCCTAGCCT CCTCCATCAG CACTACAGTT AATGCATCTG CTGTGACCAA AGGTCCCCTT
    GTTCCTGCAG TTAAAGTCAA GAGACATGAA ATAAAGAGTG ACCCAACTCC CCTGGGGTTT
    GAAGATGCTT TTGAGAACAC CATATTGGCC CAGACTAAAT GTAGCACTGG TTCCACCACA
    AGACCCGTAC CTAGATCTGC TTCACAGACA GGTTGGCCCA GTAGGCAAAT GCCATCTCTA
    GACACATTTG AAGACTTTGA AGCCATTCCT TCCAGCATGG ATGAACTCTC CAACTCTTCA
    GACATGGAGG AAGAAACCAA CCCTAACAAT GGGACCAATC TTTTTCGAGT AGAAGGCAGT
    TTTGACAGCT GTTTCCCAGA TTCTCTTACA CTTGAAGATG GAGATCTAGT GCAGTTTGTG
    CAGGAAGGAG AAAATGGGCA ATGGTTAGTG AAGAAATTGG TCTCGGAGAA AAGTGAATGG
    GTTCCTTCCA GTATATTGCA GCCAGCAGAG GGGGACACTA ACAACATAAT TAAGAACAGC
    AATGCAGACA TGTACAATGA TTCCTGCAGC ACGGCTTCAG TTGAGTCGGA CACCAAGGAG
    AGTGAGGTGG CTAAAAGCTT CCCAGCAGAA CAGAGGGCTG GCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12715
    Clone ID Related Accession (Same CDS sequence) XM_004943443.2 , XM_004943443.3
    Accession Version XM_004943443.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004943443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943500.1
    CDS154..3825
    Translation

    Target ORF information:

    RefSeq Version XM_004943443.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943443.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12715
    Clone ID Related Accession (Same CDS sequence) XM_004943443.2 , XM_004943443.3
    Accession Version XM_004943443.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3672bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004943443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004943500.1
    CDS138..3809
    Translation

    Target ORF information:

    RefSeq Version XM_004943443.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004943443.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTCC CCTTGTTCCT GCAGTTAAAG TCAAGAGACA TGAAATAAAG
    AGTGACCCAA CTCCCCTGGG GTTTGAAGAT GCTTTTGAGA ACACCATATT GGCCCAGACT
    AAATGTAGCA CTGGTTCCAC CACAAGACCC GTACCTAGAT CTGCTTCACA GACAGGTTGG
    CCCAGTAGGC AAATGCCATC TCTAGACACA TTTGAAGACT TTGAAGCCAT TCCTTCCAGC
    ATGGATGAAC TCTCCAACTC TTCAGACATG GAGGAAGAAA CCAACCCTAA CAATGGGACC
    AATCTTTTTC GAGTAGAAGG CAGTTTTGAC AGCTGTTTCC CAGATTCTCT TACACTTGAA
    GATGGAGATC TAGTGCAGTT TGTGCAGGAA GGAGAAAATG GGCAATGGTT AGTGAAGAAA
    TTGGTCTCGG AGAAAAGTGA ATGGGTTCCT TCCAGTATAT TGCAGCCAGC AGAGGGGGAC
    ACTAACAACA TAATTAAGAA CAGCAATGCA GACATGTACA ATGATTCCTG CAGCACGGCT
    TCAGTTGAGT CGGACACCAA GGAGAGTGAG GTGGCTAAAA GCTTCCCAGC AGAACAGAGG
    GCTGGCAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12716
    Clone ID Related Accession (Same CDS sequence) XM_422768.5 , XM_422768.6
    Accession Version XM_422768.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455915.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_422768.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTTG GCCCAGTAGG CAAATGCCAT CTCTAGACAC ATTTGAAGAC
    TTTGAAGCCA TTCCTTCCAG CATGGATGAA CTCTCCAACT CTTCAGACAT GGAGGAAGAA
    ACCAACCCTA ACAATGGGAC CAATCTTTTT CGAGTAGAAG GCAGTTTTGA CAGCTGTTTC
    CCAGATTCTC TTACACTTGA AGATGGAGAT CTAGTGCAGT TTGTGCAGGA AGGAGAAAAT
    GGGCAATGGT TAGTGAAGAA ATTGGTCTCG GAGAAAAGTG AATGGGTTCC TTCCAGTATA
    TTGCAGCCAG CAGAGGGGGA CACTAACAAC ATAATTAAGA ACAGCAATGC AGACATGTAC
    AATGATTCCT GCAGCACGGC TTCAGTTGAG TCGGACACCA AGGAGAGTGA GGTGGCTAAA
    AGCTTCCCAG CAGAACAGAG GGCTGGCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_422768.4
    CDS150..3662
    Translation

    Target ORF information:

    RefSeq Version XM_422768.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_422768.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTTG GCCCAGTAGG CAAATGCCAT CTCTAGACAC ATTTGAAGAC
    TTTGAAGCCA TTCCTTCCAG CATGGATGAA CTCTCCAACT CTTCAGACAT GGAGGAAGAA
    ACCAACCCTA ACAATGGGAC CAATCTTTTT CGAGTAGAAG GCAGTTTTGA CAGCTGTTTC
    CCAGATTCTC TTACACTTGA AGATGGAGAT CTAGTGCAGT TTGTGCAGGA AGGAGAAAAT
    GGGCAATGGT TAGTGAAGAA ATTGGTCTCG GAGAAAAGTG AATGGGTTCC TTCCAGTATA
    TTGCAGCCAG CAGAGGGGGA CACTAACAAC ATAATTAAGA ACAGCAATGC AGACATGTAC
    AATGATTCCT GCAGCACGGC TTCAGTTGAG TCGGACACCA AGGAGAGTGA GGTGGCTAAA
    AGCTTCCCAG CAGAACAGAG GGCTGGCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa12716
    Clone ID Related Accession (Same CDS sequence) XM_422768.5 , XM_422768.6
    Accession Version XM_422768.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3513bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product probable guanine nucleotide exchange factor MCF2L2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006096.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_422768.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTTG GCCCAGTAGG CAAATGCCAT CTCTAGACAC ATTTGAAGAC
    TTTGAAGCCA TTCCTTCCAG CATGGATGAA CTCTCCAACT CTTCAGACAT GGAGGAAGAA
    ACCAACCCTA ACAATGGGAC CAATCTTTTT CGAGTAGAAG GCAGTTTTGA CAGCTGTTTC
    CCAGATTCTC TTACACTTGA AGATGGAGAT CTAGTGCAGT TTGTGCAGGA AGGAGAAAAT
    GGGCAATGGT TAGTGAAGAA ATTGGTCTCG GAGAAAAGTG AATGGGTTCC TTCCAGTATA
    TTGCAGCCAG CAGAGGGGGA CACTAACAAC ATAATTAAGA ACAGCAATGC AGACATGTAC
    AATGATTCCT GCAGCACGGC TTCAGTTGAG TCGGACACCA AGGAGAGTGA GGTGGCTAAA
    AGCTTCCCAG CAGAACAGAG GGCTGGCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_422768.4
    CDS135..3647
    Translation

    Target ORF information:

    RefSeq Version XM_422768.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus MCF.2 cell line derived transforming sequence-like 2 (MCF2L2), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_422768.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGCTTCTC GGTGTCGGAA ATGTGGACAA CAGGTGCCCA GGAATCTACC TTTCACTGCT 
    GTTCGGATAA CAGAGGGAGA AATGAAATCT TACCATGATT TCATAGGAGG ATGCATTAGA
    CTGGAGTGTA TGGCAAAGAA AGATGAAATC ATGCAGCAGG AGATACGACC GCTGGTTGCA
    GTGGATATAA TAGAACAGCT ACACAGGCAA TTTGCTATCT TGTCAGGAGG AAGAGGGAAG
    GATGGAGCGC CAATTATTAC CTTCCCAGAG TTTGCAGGAT TCAGTGAAAT ACCCGATGAG
    GATTTTCTGA ATGTGGTCAC GTACCTAACC AGCATTCCCA GTCTGGAGGC TGCCAGCATT
    GGATTCATCA TCATCATAGA CAGACGACGA GACAAATGGA GCGCAGTCAA GGCATCCCTG
    ACACGGATAG CAGGAGCATT TCCAGGAAAC CTACAGCTGG TGTTTGTCCT GCGTCCCTCA
    CGCTTTATCC AAAGGGCAAT CGCTGACATT GGCATTAAAC TCTATCGAGA TGACTTTAAA
    ATGAAGGTAC CGATCATCAT GCTAAATTCT GTCTCTGATC TTCATGGCTA TATTGATAAA
    TGTCAACTGA CCCGGGAGCT GGGAGGAACC CTGGAGTACG GCCACAGTCA GTGGATACAT
    CACCGAACTG CTATTGAAAA CTTTGCAATG ACAGTGAAAA CAACAGCACA GATGCTACAG
    ACCTTTGGAA CGGACTTAGC AGAGACTGAA CTTCCCAATG ATGTGCAGTG TACAGAGGAG
    CTCCTATCTG CACACACTGA CCACCATAGC AAGCTGAAGG ATGAGCTGAA ACTAGCTGTG
    AAGCAGGGGG CCACACTACT GACGTGCATT AGGGAGCCAG TCACCCGAAG TGCCAACAGC
    AAGCTCAGTC CAGATGAACT GGAAAACGTG GCAACAGTGG AAAGGTTGTT GGCTCAGTTG
    GATGAAACAG AAAAGGCTTT TGATCAATTT TGGACCAAGC ATCACCTAAA ACTAGAACAA
    TGCTTGCAGC TTCGACACTT TGAACATGAT TTTAGAGAAG TAAAATTAAC ATTGGATAAT
    CTCATGGAAG CACAAGCTGG TTTTTCTGAC GTTGGAGACA GTGTGACTCG AGTAGAACAC
    CTCCTAAGGG AACAGAAGCA ACTGGAAGAA AAAGGGCAGG AACCTTTGGA GAAAGCCCAG
    ACTCTAGCTC TCCATGGAGA ACAGCTCATC CAAAACAACC ATTATGCAGT TGACTCCATT
    AGACCAAAGT GTGTTGAACT CAGGCGTATT TGCGATGACT TTACTAATGA GACCAAGAAA
    AAGTATGATA TTTTGGGGAA GTCTCTTGAG CTGCATAAGC AATTAGATAA GGCAAGCCAA
    TGGTGTGAAG CTGGAATCTA CCTCTTAGCT TCCCAAGCTG TGGACAAGTG CCAGTCACAA
    GAGGGAGCTG AAACTGCACT CGTTGAAATA GAGAAGTTCC TGGCAACAGC AAAGGAGCAC
    CAACTCTCTA ACCCAAAGGA GTTCTACAAT CAGTTTGACA CGATTCTCAC ACCTGAAATA
    AAGGCCAACG CCCAAAGGAT TTTACAAAAG CTAGAAGATG TACAAGAGAT GTTTGACAAG
    AGGCAAGTGA GTCTGAAGAA GCTAGCAGCC AAACAGACCC GTCCAGTGCA ACCTGTTGCC
    CCACATCCTG AATCTTCCCC AAAACGAGCA TCACCAAAGA CTACCAGACC AGCAGGAGTA
    GCTACCAACA GGAGGTCCGC AGAAATTACT TGTCCTCCAA AGTCCATCTC TGAGGCAGAG
    TTATCAAAAA AGAAAAGCAT TAAGAAGACT AAAGGTGGAA TTAAGATTGA AGTGATGCAT
    GAAGCGAGCC AAGGGGGATC TAGCAGAGTC TTGGTTATGA GTGAAAGCGA TGAGAACCTG
    TCGACGAGGA GGAGCCACAT AATCAATGAG CTGATAGAAA CCGAACGCGT TTATGTGGAA
    GAACTTCAAA GCATAATAGA GGGATATGCT TCAGAAATGG ACAATCCAAG TTTATTACAT
    CTGATTCCAT CAGTGCTCCA GAACAAAAAG GATATTCTAT TTGGGAACCT CCCAGAAATC
    TATGAATTCC ACAACAGGAT TTTCCTTAAA GAGTTAGAAA ATTGCATTGA AAATCCTGAG
    CTCCTTGCCC GATGCTTTTT GAAAAGGAAA GAGGACCTTC AAATATATGA AAAATACTGT
    CAGAACAAGC CAAGGTCTGA AGCTCTCTGG AGACAGTGTG GAGACAGCAT CTTTTTTCAG
    GAATGTCAGC GTAAATTGGA TCATAAGCTC TCACTAGATG CGTATCTTTT AAAGCCAGTC
    CAGAGAATCA CCAAGTACCA GCTACTATTG AAGGAAATGC TGAAATGTAG CAAGAATTCA
    GAAGGTACTG CTGAACTGGA AGAGGCCTTG GCCACAGTGC TTGATATCAT CAAATCCGTA
    AATGACTCCA TGCACCAGAT AGCAATCACA GGATATGAGG GGGACGTAAG TGAGCTTGGT
    AAGCTGTTGA TGCAAGGTTC CTTCAACGTG TGGACTGACC ACAAGAAGGG TCACAACAAG
    GTGAAGGACT TGGCAAGATT CAAGCCGATG CAGAGACACC TCTTCCTCTA CACAAAGATG
    CTGCTTTTTT GCAAGAAACG AGAGGAAAAC ACAGATGGTC ATGAGAAGAC AGCTTCATAC
    AGCTTTAAAA ATTCATTAAA GATGAGCACA GTGGGCATCA CAGAAAATGT AAAAGGAGAC
    AACAAGAAGT TTGAGATCTG GTATAATGGC AGAGAAGAAG TCTATATCAT TCAGGCATCT
    TCTGTTGAAC TGAAAAACAC TTGGATTTCC GAGATACGCA AAGTTCTAAC AGGACAACTG
    GAAGCATGTA GAGAAGCAAG TCAACTACAC CAAAGAATCA CCGAGAGTGT GTACCATGCA
    CCAATGAATT CCTCCAATAT AACCAGGTAT AGCAGAAAGT CGTCAAGCAT ATATGAAAAC
    AAATCCGAGA CATCAAACGT GGAAGCATCT AACAATAAAA ACAGGAATTC CAGTCCCAGT
    GCCAGACAAA AGAGAGGTTG GCCCAGTAGG CAAATGCCAT CTCTAGACAC ATTTGAAGAC
    TTTGAAGCCA TTCCTTCCAG CATGGATGAA CTCTCCAACT CTTCAGACAT GGAGGAAGAA
    ACCAACCCTA ACAATGGGAC CAATCTTTTT CGAGTAGAAG GCAGTTTTGA CAGCTGTTTC
    CCAGATTCTC TTACACTTGA AGATGGAGAT CTAGTGCAGT TTGTGCAGGA AGGAGAAAAT
    GGGCAATGGT TAGTGAAGAA ATTGGTCTCG GAGAAAAGTG AATGGGTTCC TTCCAGTATA
    TTGCAGCCAG CAGAGGGGGA CACTAACAAC ATAATTAAGA ACAGCAATGC AGACATGTAC
    AATGATTCCT GCAGCACGGC TTCAGTTGAG TCGGACACCA AGGAGAGTGA GGTGGCTAAA
    AGCTTCCCAG CAGAACAGAG GGCTGGCAGC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.