mcf2l.2 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_012963327.2	XP_012818781.1	guanine nucleotide exchange factor DBS isoform X1
XM_002937839.4	XP_002937885.2	guanine nucleotide exchange factor DBS isoform X3
XM_018094369.1	XP_017949858.1	guanine nucleotide exchange factor DBS isoform X4
XM_031902051.1	XP_031757911.1	guanine nucleotide exchange factor DBS isoform X5
XM_012963330.3	XP_012818784.1	guanine nucleotide exchange factor DBS isoform X7
XM_031902049.1	XP_031757909.1	guanine nucleotide exchange factor DBS isoform X1
XM_012963327.3	XP_012818781.1	guanine nucleotide exchange factor DBS isoform X2
XM_031902052.1	XP_031757912.1	guanine nucleotide exchange factor DBS isoform X6
XM_018094368.1	XP_017949857.1	guanine nucleotide exchange factor DBS isoform X2
XM_012963330.2	XP_012818784.1	guanine nucleotide exchange factor DBS isoform X5
XM_031902050.1	XP_031757910.1	guanine nucleotide exchange factor DBS isoform X3
XM_002937839.5	XP_002937885.2	guanine nucleotide exchange factor DBS isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa10132	XM_002937839.4 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa33159	XM_012963327.2 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa33162	XM_012963330.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa44731	XM_018094368.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa44732	XM_018094369.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58343	XM_031902049.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58344	XM_012963327.3 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58345	XM_031902050.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58346	XM_002937839.5 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58347	XM_031902051.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa58348	XM_031902052.1 Latest version!	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OXa33162	XM_012963330.2	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
Hide Old Accession Versions >>

mcf2l.2 ( XM_002937839.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937885.2 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa10132
Clone ID Related Accession (Same CDS sequence)	XM_002937839.4
Accession Version	XM_002937839.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002937839.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGAA 
TCTCAAGAGA AAGAATCTAC TCGGCGATTC TCCCTAGCTT CTTCTGTGAG CTCAACATCT 
ATTAAAGTTC CTACTCGTAC AAAAGGTCCA CTTGGCCCTG ATGTGAAAGC AAAGCGACAT 
GAGATAAAGA GTGACCCAAC CCCCCTTGGT TTCGAAGGCT GGCCGAGACA CATGAACTCT 
ATGGAAACTT TCCAAGACTT TTCAGCTATC CCATCCAGCA ATGATGAACT TTCCAATTCT 
TCAGATGTTG ATGAGGATAT AACAAATAGC AAAAGATCCA ATCTGTTTCG TGTGGAAGGA 
TGTTTGGAAG TATGTTCCCC CAGTGCGCTC ACACTTCAGC ATGGTGACCT TGTACAAGTG 
GAACAATGTG GAGACAGTGG ACAGTGGTTA GTAAAAGAAG TTGTTTCCAA GAAAACTGCA 
TGGGTACCTT CCAACATGTT AATTCCTGCC AGGAGCGATA TTAAGAACAT CTGCCAAGAG 
GATAACAGCA CAGCTTACAG TGAAGCATGT CAAATATCAC TGGCATCAAC AAGCATGAAA 
GAGAAGCAGA ATGAAAGGAC ACGAAGCTTG GTGGCTGAGC AGAAGGCAGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937885.2
CDS	80..3856
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_002937839.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937839.4

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGAA 
TCTCAAGAGA AAGAATCTAC TCGGCGATTC TCCCTAGCTT CTTCTGTGAG CTCAACATCT 
ATTAAAGTTC CTACTCGTAC AAAAGGTCCA CTTGGCCCTG ATGTGAAAGC AAAGCGACAT 
GAGATAAAGA GTGACCCAAC CCCCCTTGGT TTCGAAGGCT GGCCGAGACA CATGAACTCT 
ATGGAAACTT TCCAAGACTT TTCAGCTATC CCATCCAGCA ATGATGAACT TTCCAATTCT 
TCAGATGTTG ATGAGGATAT AACAAATAGC AAAAGATCCA ATCTGTTTCG TGTGGAAGGA 
TGTTTGGAAG TATGTTCCCC CAGTGCGCTC ACACTTCAGC ATGGTGACCT TGTACAAGTG 
GAACAATGTG GAGACAGTGG ACAGTGGTTA GTAAAAGAAG TTGTTTCCAA GAAAACTGCA 
TGGGTACCTT CCAACATGTT AATTCCTGCC AGGAGCGATA TTAAGAACAT CTGCCAAGAG 
GATAACAGCA CAGCTTACAG TGAAGCATGT CAAATATCAC TGGCATCAAC AAGCATGAAA 
GAGAAGCAGA ATGAAAGGAC ACGAAGCTTG GTGGCTGAGC AGAAGGCAGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_012963327.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818781.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa33159
Clone ID Related Accession (Same CDS sequence)	XM_012963327.2
Accession Version	XM_012963327.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963327.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGAATCTC AAGAGAAAGA ATCTACTCGG 
CGATTCTCCC TAGCTTCTTC TGTGAGCTCA ACATCTATTA AAGTTCCTAC TCGTACAAAA 
GGTCCACTTG GCCCTGATGT GAAAGCAAAG CGACATGAGA TAAAGAGTGA CCCAACCCCC 
CTTGGTTTCG AAGGCTGGCC GAGACACATG AACTCTATGG AAACTTTCCA AGACTTTTCA 
GCTATCCCAT CCAGCAATGA TGAACTTTCC AATTCTTCAG ATGTTGATGA GGATATAACA 
AATAGCAAAA GATCCAATCT GTTTCGTGTG GAAGGATGTT TGGAAGTATG TTCCCCCAGT 
GCGCTCACAC TTCAGCATGG TGACCTTGTA CAAGTGGAAC AATGTGGAGA CAGTGGACAG 
TGGTTAGTAA AAGAAGTTGT TTCCAAGAAA ACTGCATGGG TACCTTCCAA CATGTTAATT 
CCTGCCAGGA GCGATATTAA GAACATCTGC CAAGAGGATA ACAGCACAGC TTACAGTGAA 
GCATGTCAAA TATCACTGGC ATCAACAAGC ATGAAAGAGA AGCAGAATGA AAGGACACGA 
AGCTTGGTGG CTGAGCAGAA GGCAGTGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818781.1
CDS	80..3952
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_012963327.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963327.2

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGAATCTC AAGAGAAAGA ATCTACTCGG 
CGATTCTCCC TAGCTTCTTC TGTGAGCTCA ACATCTATTA AAGTTCCTAC TCGTACAAAA 
GGTCCACTTG GCCCTGATGT GAAAGCAAAG CGACATGAGA TAAAGAGTGA CCCAACCCCC 
CTTGGTTTCG AAGGCTGGCC GAGACACATG AACTCTATGG AAACTTTCCA AGACTTTTCA 
GCTATCCCAT CCAGCAATGA TGAACTTTCC AATTCTTCAG ATGTTGATGA GGATATAACA 
AATAGCAAAA GATCCAATCT GTTTCGTGTG GAAGGATGTT TGGAAGTATG TTCCCCCAGT 
GCGCTCACAC TTCAGCATGG TGACCTTGTA CAAGTGGAAC AATGTGGAGA CAGTGGACAG 
TGGTTAGTAA AAGAAGTTGT TTCCAAGAAA ACTGCATGGG TACCTTCCAA CATGTTAATT 
CCTGCCAGGA GCGATATTAA GAACATCTGC CAAGAGGATA ACAGCACAGC TTACAGTGAA 
GCATGTCAAA TATCACTGGC ATCAACAAGC ATGAAAGAGA AGCAGAATGA AAGGACACGA 
AGCTTGGTGG CTGAGCAGAA GGCAGTGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_012963330.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818784.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X7, mRNA.

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CloneID	OXa33162
Clone ID Related Accession (Same CDS sequence)	XM_012963330.3 , XM_012963330.2
Accession Version	XM_012963330.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963330.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGGC 
TGGCCGAGAC ACATGAACTC TATGGAAACT TTCCAAGACT TTTCAGCTAT CCCATCCAGC 
AATGATGAAC TTTCCAATTC TTCAGATGTT GATGAGGATA TAACAAATAG CAAAAGATCC 
AATCTGTTTC GTGTGGAAGG ATGTTTGGAA GTATGTTCCC CCAGTGCGCT CACACTTCAG 
CATGGTGACC TTGTACAAGT GGAACAATGT GGAGACAGTG GACAGTGGTT AGTAAAAGAA 
GTTGTTTCCA AGAAAACTGC ATGGGTACCT TCCAACATGT TAATTCCTGC CAGGAGCGAT 
ATTAAGAACA TCTGCCAAGA GGATAACAGC ACAGCTTACA GTGAAGCATG TCAAATATCA 
CTGGCATCAA CAAGCATGAA AGAGAAGCAG AATGAAAGGA CACGAAGCTT GGTGGCTGAG 
CAGAAGGCAG TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818784.1
CDS	74..3691
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_012963330.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963330.3

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGGC 
TGGCCGAGAC ACATGAACTC TATGGAAACT TTCCAAGACT TTTCAGCTAT CCCATCCAGC 
AATGATGAAC TTTCCAATTC TTCAGATGTT GATGAGGATA TAACAAATAG CAAAAGATCC 
AATCTGTTTC GTGTGGAAGG ATGTTTGGAA GTATGTTCCC CCAGTGCGCT CACACTTCAG 
CATGGTGACC TTGTACAAGT GGAACAATGT GGAGACAGTG GACAGTGGTT AGTAAAAGAA 
GTTGTTTCCA AGAAAACTGC ATGGGTACCT TCCAACATGT TAATTCCTGC CAGGAGCGAT 
ATTAAGAACA TCTGCCAAGA GGATAACAGC ACAGCTTACA GTGAAGCATG TCAAATATCA 
CTGGCATCAA CAAGCATGAA AGAGAAGCAG AATGAAAGGA CACGAAGCTT GGTGGCTGAG 
CAGAAGGCAG TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_012963330.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818784.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X5, mRNA.

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CloneID	OXa33162
Clone ID Related Accession (Same CDS sequence)	XM_012963330.3 , XM_012963330.2
Accession Version	XM_012963330.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012963330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGGC 
TGGCCGAGAC ACATGAACTC TATGGAAACT TTCCAAGACT TTTCAGCTAT CCCATCCAGC 
AATGATGAAC TTTCCAATTC TTCAGATGTT GATGAGGATA TAACAAATAG CAAAAGATCC 
AATCTGTTTC GTGTGGAAGG ATGTTTGGAA GTATGTTCCC CCAGTGCGCT CACACTTCAG 
CATGGTGACC TTGTACAAGT GGAACAATGT GGAGACAGTG GACAGTGGTT AGTAAAAGAA 
GTTGTTTCCA AGAAAACTGC ATGGGTACCT TCCAACATGT TAATTCCTGC CAGGAGCGAT 
ATTAAGAACA TCTGCCAAGA GGATAACAGC ACAGCTTACA GTGAAGCATG TCAAATATCA 
CTGGCATCAA CAAGCATGAA AGAGAAGCAG AATGAAAGGA CACGAAGCTT GGTGGCTGAG 
CAGAAGGCAG TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818784.1
CDS	80..3697
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_012963330.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963330.2

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGGC 
TGGCCGAGAC ACATGAACTC TATGGAAACT TTCCAAGACT TTTCAGCTAT CCCATCCAGC 
AATGATGAAC TTTCCAATTC TTCAGATGTT GATGAGGATA TAACAAATAG CAAAAGATCC 
AATCTGTTTC GTGTGGAAGG ATGTTTGGAA GTATGTTCCC CCAGTGCGCT CACACTTCAG 
CATGGTGACC TTGTACAAGT GGAACAATGT GGAGACAGTG GACAGTGGTT AGTAAAAGAA 
GTTGTTTCCA AGAAAACTGC ATGGGTACCT TCCAACATGT TAATTCCTGC CAGGAGCGAT 
ATTAAGAACA TCTGCCAAGA GGATAACAGC ACAGCTTACA GTGAAGCATG TCAAATATCA 
CTGGCATCAA CAAGCATGAA AGAGAAGCAG AATGAAAGGA CACGAAGCTT GGTGGCTGAG 
CAGAAGGCAG TGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_018094368.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949857.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa44731
Clone ID Related Accession (Same CDS sequence)	XM_018094368.1
Accession Version	XM_018094368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGAT TGTAATGATA 
AACTCAGTTT CTGATCTTCA TGGATACATT GATAAAAGCC AGCTAACCAC TGAGCTGGGA 
GGTACCTTGG AATACAGCCA TAGTCAATGG ATACATCATA GAACAGCCAT TGAGAGCTTT 
GCTCAAGTTG TGAAAACGAC AGCACAGAAC CTGCAGACCT TTGGAACAAG CCTTGCAGAG 
ACTGAGCTTC CAAATGATGT ACTGTGCACA GAGGAGCTGC TGCAGTCCCA CACAGAGCAA 
CAGATCAGTC TAAAAAATGA CCTGAACTTG GCAATGCAAC AAGGTGGCAC GCTGCTCTCA 
TGCATAAAGG AACCAGTATC AAAAGACCCA AGCTACAAAC TAAATCCAGA TGAGGTTGAG 
AACATGGCTA CAGTTGAAAG ACTTCTGGCT CAGCTAGATG AAACAGAAAA AGCTTTTGGT 
CACTTCTGGA GTCGGCATCA ACTAAAGTTA GAACAATGTT TGCAACTGCG ACACTTTGAA 
CATGATTTCA GAGAGTTGAA GCTAACTTTG GATGGACTGA TGGAGATCCA AGCTGCATTT 
GCAGACATTG GAGACAGTGT GTCACGGGTG GAATACCTTA TTAAGGAGCT AAAAAAGCTG 
GAAGAGAAAG GACAGGGAAT GTTGGAGAAA GCCCAGCTGC ATGCTCTTCA CGGTGACCAG 
CTTATCCAAA ACAACCACTA TGCTGTGGAC TCTATTAGAC CAAAATGCAT AGAACTCAGG 
CGAATCTGTG ATGACTTTAC CAATGAGACA AAGAAAAAGT ATGACATATT GGCAAAATCA 
CTGGAGCTGC ACCAGCAACT TGACGAGGCC AACAAGTGGT GTGAAGATGG AATCTACCTT 
TTGGCTTCCC AAGCTGTGGA TAAGTGCCAG TCACAGGAAG GTGCAGAGTC TGCTTTACGG 
GACATAGAAC TGTTCATTGA AAGCTCAAGA GGACACCAGC TTTCCAATCC AACAGATTTC 
TACAATAAAT ATGATGCAAT TCTCACTCTT GAAATCAAAG CAAATGTTCA GAAAGTTTTG 
CAGAAGGTGG CTGATGTTCA GGAGATGTTT AATAAGAGAC AAGCAAGCCT CAAAAAGCTG 
GCAGCCAAAC AGACAAGACC TATCCAGCCT GTGGCCCCAC ATCCAGAGTC CTCACCAAAG 
AGGTCATCTC CAAAGACTGT ACGACCCACC TCACTGTCCA CAACCCGAAG AGCAGCTGAT 
GTGCCATGCG TGCCTCCCAA GTCTCTCTCT GAAGCAGACC TGTCCAGGAA AAGGAACAAT 
AAGAAAACCA AAGCAGGAAT AAAGATTGAA GTAATGCATG AAGCTAGTCA GGGTGGCACA 
AGCCGCACAT TGGTGATGAG TGAGAGTGAA GAAAACCTCT CTTCAAGAAA GAGCCACATC 
ATAAATGAGT TAATTGAAAC AGAACGTGTC TATGTTGAAG AGCTTCAAAG TATCCTAGAG 
GGTTATGCAT CATCTCTGGA AAATCCTGAA ATGATTGGTT TGATACCAGT GGCATTGCAA 
AACAATAAGG AAGTTCTCTT TGGAAACCTT TCAGAAATTT ATGAATTTCA CAAAAGAATT 
TTCCTCAAAG AATTAGAGAA CTGTATTGAA AATGCTGATT TGCTTGGAAC ATGTTTCTTA 
AAAAGGAAAG AGGACCTTCA GATTTATGAA AAGTACTGCC AAAATAAACC AAGGTCGGAA 
GCAATCTGGA GACAGTGTGC AGAGAGCATT TTTTTTCAGG AATGCCAGCG TAGACTGGAT 
CATAAGCTGT CTTTAGATGC TTATCTTCTT AAGCCTGTCC AAAGGATCAC AAAATATCAG 
CTGCTTTTAA AGGAAATGTT GAAATGCAGT AAGAATTCTG AAGGGACAGC TGAACTAGAA 
GAAGCACTTG CCACAGTGTT GGACATAATT AAGTCAGTGA ATGACTCAAT GCACCAAATA 
GCTATAACAG GATATGAGGG GGATCTGAAC GACCTTGGTA AGCTGCTTAT GCAGGGCTCA 
TTTAATGTGT GGACTGATCA CAAGAAAGGG CACAACAAGG TAAAAGACTT GGCCCGATTC 
AAACCCATGC AACGGCATCT CTTTTTATAT GCTAAGGCCC TACTGTTTTG CAAGAAGAGG 
GAAGAGAACA GTGAAGGACA TGAAAAATCT CAGTCTTACA GTTTCAAGCA TATTTTAAAG 
ATGAGTTCAG TTGGCATCAC TGAAAATGTT AAAGGGGACA ACAAGAAGTT TGAAGTATGG 
TACAATGGCA GAGAAGAAGT CTACATTATT CAGGCATCAA CTATTGAGCT GAAAAACCTT 
TGGGTTTCTG AAATCAGAAA AGTCCTTACA GGCCAACTGG AAGCTTGCAG AGAAGCAAGC 
CAATTACATC AAAGGATCAC AGAGAGCGTA TACCACGCAC CAATGAACTC CTCATTTATT 
GCCAGGTCCA ATAAGACTTC AGGTAACAGC CAGAACAAAG GAGAATTGCC AAGTCTTGAA 
GTGTCCAATA AGAAGAACAG GACTTCTAGT CCAACTGGAA GACAAAAGAA AGCTGATAAT 
CCTGCCAGTC ATAATCTTGA GCGCTCAGCT CTTGCTAGAA AAAGATTCAC ATTACAGGGT 
CTTTCCAATC GGAGAGCTCC ACACAAAGAA TCTCAAGAGA AAGAATCTAC TCGGCGATTC 
TCCCTAGCTT CTTCTGTGAG CTCAACATCT ATTAAAGTTC CTACTCGTAC AAAAGGTCCA 
CTTGGCCCTG ATGTGAAAGC AAAGCGACAT GAGATAAAGA GTGACCCAAC CCCCCTTGGT 
TTCGAAGGCT GGCCGAGACA CATGAACTCT ATGGAAACTT TCCAAGACTT TTCAGCTATC 
CCATCCAGCA ATGATGAACT TTCCAATTCT TCAGATGTTG ATGAGGATAT AACAAATAGC 
AAAAGATCCA ATCTGTTTCG TGTGGAAGGA TGTTTGGAAG TATGTTCCCC CAGTGCGCTC 
ACACTTCAGC ATGGTGACCT TGTACAAGTG GAACAATGTG GAGACAGTGG ACAGTGGTTA 
GTAAAAGAAG TTGTTTCCAA GAAAACTGCA TGGGTACCTT CCAACATGTT AATTCCTGCC 
AGGAGCGATA TTAAGAACAT CTGCCAAGAG GATAACAGCA CAGCTTACAG TGAAGCATGT 
CAAATATCAC TGGCATCAAC AAGCATGAAA GAGAAGCAGA ATGAAAGGAC ACGAAGCTTG 
GTGGCTGAGC AGAAGGCAGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949857.1
CDS	80..3946
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_018094368.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094368.1

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGAT TGTAATGATA 
AACTCAGTTT CTGATCTTCA TGGATACATT GATAAAAGCC AGCTAACCAC TGAGCTGGGA 
GGTACCTTGG AATACAGCCA TAGTCAATGG ATACATCATA GAACAGCCAT TGAGAGCTTT 
GCTCAAGTTG TGAAAACGAC AGCACAGAAC CTGCAGACCT TTGGAACAAG CCTTGCAGAG 
ACTGAGCTTC CAAATGATGT ACTGTGCACA GAGGAGCTGC TGCAGTCCCA CACAGAGCAA 
CAGATCAGTC TAAAAAATGA CCTGAACTTG GCAATGCAAC AAGGTGGCAC GCTGCTCTCA 
TGCATAAAGG AACCAGTATC AAAAGACCCA AGCTACAAAC TAAATCCAGA TGAGGTTGAG 
AACATGGCTA CAGTTGAAAG ACTTCTGGCT CAGCTAGATG AAACAGAAAA AGCTTTTGGT 
CACTTCTGGA GTCGGCATCA ACTAAAGTTA GAACAATGTT TGCAACTGCG ACACTTTGAA 
CATGATTTCA GAGAGTTGAA GCTAACTTTG GATGGACTGA TGGAGATCCA AGCTGCATTT 
GCAGACATTG GAGACAGTGT GTCACGGGTG GAATACCTTA TTAAGGAGCT AAAAAAGCTG 
GAAGAGAAAG GACAGGGAAT GTTGGAGAAA GCCCAGCTGC ATGCTCTTCA CGGTGACCAG 
CTTATCCAAA ACAACCACTA TGCTGTGGAC TCTATTAGAC CAAAATGCAT AGAACTCAGG 
CGAATCTGTG ATGACTTTAC CAATGAGACA AAGAAAAAGT ATGACATATT GGCAAAATCA 
CTGGAGCTGC ACCAGCAACT TGACGAGGCC AACAAGTGGT GTGAAGATGG AATCTACCTT 
TTGGCTTCCC AAGCTGTGGA TAAGTGCCAG TCACAGGAAG GTGCAGAGTC TGCTTTACGG 
GACATAGAAC TGTTCATTGA AAGCTCAAGA GGACACCAGC TTTCCAATCC AACAGATTTC 
TACAATAAAT ATGATGCAAT TCTCACTCTT GAAATCAAAG CAAATGTTCA GAAAGTTTTG 
CAGAAGGTGG CTGATGTTCA GGAGATGTTT AATAAGAGAC AAGCAAGCCT CAAAAAGCTG 
GCAGCCAAAC AGACAAGACC TATCCAGCCT GTGGCCCCAC ATCCAGAGTC CTCACCAAAG 
AGGTCATCTC CAAAGACTGT ACGACCCACC TCACTGTCCA CAACCCGAAG AGCAGCTGAT 
GTGCCATGCG TGCCTCCCAA GTCTCTCTCT GAAGCAGACC TGTCCAGGAA AAGGAACAAT 
AAGAAAACCA AAGCAGGAAT AAAGATTGAA GTAATGCATG AAGCTAGTCA GGGTGGCACA 
AGCCGCACAT TGGTGATGAG TGAGAGTGAA GAAAACCTCT CTTCAAGAAA GAGCCACATC 
ATAAATGAGT TAATTGAAAC AGAACGTGTC TATGTTGAAG AGCTTCAAAG TATCCTAGAG 
GGTTATGCAT CATCTCTGGA AAATCCTGAA ATGATTGGTT TGATACCAGT GGCATTGCAA 
AACAATAAGG AAGTTCTCTT TGGAAACCTT TCAGAAATTT ATGAATTTCA CAAAAGAATT 
TTCCTCAAAG AATTAGAGAA CTGTATTGAA AATGCTGATT TGCTTGGAAC ATGTTTCTTA 
AAAAGGAAAG AGGACCTTCA GATTTATGAA AAGTACTGCC AAAATAAACC AAGGTCGGAA 
GCAATCTGGA GACAGTGTGC AGAGAGCATT TTTTTTCAGG AATGCCAGCG TAGACTGGAT 
CATAAGCTGT CTTTAGATGC TTATCTTCTT AAGCCTGTCC AAAGGATCAC AAAATATCAG 
CTGCTTTTAA AGGAAATGTT GAAATGCAGT AAGAATTCTG AAGGGACAGC TGAACTAGAA 
GAAGCACTTG CCACAGTGTT GGACATAATT AAGTCAGTGA ATGACTCAAT GCACCAAATA 
GCTATAACAG GATATGAGGG GGATCTGAAC GACCTTGGTA AGCTGCTTAT GCAGGGCTCA 
TTTAATGTGT GGACTGATCA CAAGAAAGGG CACAACAAGG TAAAAGACTT GGCCCGATTC 
AAACCCATGC AACGGCATCT CTTTTTATAT GCTAAGGCCC TACTGTTTTG CAAGAAGAGG 
GAAGAGAACA GTGAAGGACA TGAAAAATCT CAGTCTTACA GTTTCAAGCA TATTTTAAAG 
ATGAGTTCAG TTGGCATCAC TGAAAATGTT AAAGGGGACA ACAAGAAGTT TGAAGTATGG 
TACAATGGCA GAGAAGAAGT CTACATTATT CAGGCATCAA CTATTGAGCT GAAAAACCTT 
TGGGTTTCTG AAATCAGAAA AGTCCTTACA GGCCAACTGG AAGCTTGCAG AGAAGCAAGC 
CAATTACATC AAAGGATCAC AGAGAGCGTA TACCACGCAC CAATGAACTC CTCATTTATT 
GCCAGGTCCA ATAAGACTTC AGGTAACAGC CAGAACAAAG GAGAATTGCC AAGTCTTGAA 
GTGTCCAATA AGAAGAACAG GACTTCTAGT CCAACTGGAA GACAAAAGAA AGCTGATAAT 
CCTGCCAGTC ATAATCTTGA GCGCTCAGCT CTTGCTAGAA AAAGATTCAC ATTACAGGGT 
CTTTCCAATC GGAGAGCTCC ACACAAAGAA TCTCAAGAGA AAGAATCTAC TCGGCGATTC 
TCCCTAGCTT CTTCTGTGAG CTCAACATCT ATTAAAGTTC CTACTCGTAC AAAAGGTCCA 
CTTGGCCCTG ATGTGAAAGC AAAGCGACAT GAGATAAAGA GTGACCCAAC CCCCCTTGGT 
TTCGAAGGCT GGCCGAGACA CATGAACTCT ATGGAAACTT TCCAAGACTT TTCAGCTATC 
CCATCCAGCA ATGATGAACT TTCCAATTCT TCAGATGTTG ATGAGGATAT AACAAATAGC 
AAAAGATCCA ATCTGTTTCG TGTGGAAGGA TGTTTGGAAG TATGTTCCCC CAGTGCGCTC 
ACACTTCAGC ATGGTGACCT TGTACAAGTG GAACAATGTG GAGACAGTGG ACAGTGGTTA 
GTAAAAGAAG TTGTTTCCAA GAAAACTGCA TGGGTACCTT CCAACATGTT AATTCCTGCC 
AGGAGCGATA TTAAGAACAT CTGCCAAGAG GATAACAGCA CAGCTTACAG TGAAGCATGT 
CAAATATCAC TGGCATCAAC AAGCATGAAA GAGAAGCAGA ATGAAAGGAC ACGAAGCTTG 
GTGGCTGAGC AGAAGGCAGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_018094369.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017949858.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X4, mRNA.

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CloneID	OXa44732
Clone ID Related Accession (Same CDS sequence)	XM_018094369.1
Accession Version	XM_018094369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3627bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGAATCTC AAGAGAAAGA ATCTACTCGG 
CGATTCTCCC TAGCTTCTTC TGTGAGCTCA ACATCTATTA AAGTTCCTAC TCGTACAAAA 
GGTCCACTTG GCCCTGATGT GAAAGCAAAG CGACATGAGA TAAAGAGTGA CCCAACCCCC 
CTTGGTTTCG AAGGCTGGCC GAGACACATG AACTCTATGG AAACTTTCCA AGACTTTTCA 
GCTATCCCAT CCAGCAATGA TGAACTTTCC AATTCTTCAG ATGTTGATGA GGATATAACA 
AATAGCAAAG TTAGTAAAAG AAGTTGTTTC CAAGAAAACT GCATGGGTAC CTTCCAACAT 
GTTAATTCCT GCCAGGAGCG ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017949858.1
CDS	82..3708
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKVSKRSCFQENCMGTFQHVNSCQERY

Target ORF information:

RefSeq Version	XM_018094369.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018094369.1

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGAATCTC AAGAGAAAGA ATCTACTCGG 
CGATTCTCCC TAGCTTCTTC TGTGAGCTCA ACATCTATTA AAGTTCCTAC TCGTACAAAA 
GGTCCACTTG GCCCTGATGT GAAAGCAAAG CGACATGAGA TAAAGAGTGA CCCAACCCCC 
CTTGGTTTCG AAGGCTGGCC GAGACACATG AACTCTATGG AAACTTTCCA AGACTTTTCA 
GCTATCCCAT CCAGCAATGA TGAACTTTCC AATTCTTCAG ATGTTGATGA GGATATAACA 
AATAGCAAAG TTAGTAAAAG AAGTTGTTTC CAAGAAAACT GCATGGGTAC CTTCCAACAT 
GTTAATTCCT GCCAGGAGCG ATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_031902049.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757909.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa58343
Clone ID Related Accession (Same CDS sequence)	XM_031902049.1
Accession Version	XM_031902049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGCAGAAT CTCAGGAGAA AGAATCTACT 
CGGCGATTCT CCCTAGCTTC TTCTGTGAGC TCAACATCTA TTAAAGTTCC TACTCGTACA 
AAAGGTCCAC TTGGCCCTGA TGTGAAAGCA AAGCGACATG AGATAAAGAG TGACCCAACC 
CCCCTTGGTT TCGAAGGCTG GCCGAGACAC ATGAACTCTA TGGAAACTTT CCAAGACTTT 
TCAGCTATCC CATCCAGCAA TGATGAACTT TCCAATTCTT CAGATGTTGA TGAGGATATA 
ACAAATAGCA AAAGATCCAA TCTGTTTCGT GTGGAAGGAT GTTTGGAAGT ATGTTCCCCC 
AGTGCGCTCA CACTTCAGCA TGGTGACCTT GTACAAGTGG AACAATGTGG AGACAGTGGA 
CAGTGGTTAG TAAAAGAAGT TGTTTCCAAG AAAACTGCAT GGGTACCTTC CAACATGTTA 
ATTCCTGCCA GGAGCGATAT TAAGAACATC TGCCAAGAGG ATAACAGCAC AGCTTACAGT 
GAAGCATGTC AAATATCACT GGCATCAACA AGCATGAAAG AGAAGCAGAA TGAAAGGACA 
CGAAGCTTGG TGGCTGAGCA GAAGGCAGTG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757909.1
CDS	76..3951
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKAESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_031902049.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902049.1

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGCAGAAT CTCAGGAGAA AGAATCTACT 
CGGCGATTCT CCCTAGCTTC TTCTGTGAGC TCAACATCTA TTAAAGTTCC TACTCGTACA 
AAAGGTCCAC TTGGCCCTGA TGTGAAAGCA AAGCGACATG AGATAAAGAG TGACCCAACC 
CCCCTTGGTT TCGAAGGCTG GCCGAGACAC ATGAACTCTA TGGAAACTTT CCAAGACTTT 
TCAGCTATCC CATCCAGCAA TGATGAACTT TCCAATTCTT CAGATGTTGA TGAGGATATA 
ACAAATAGCA AAAGATCCAA TCTGTTTCGT GTGGAAGGAT GTTTGGAAGT ATGTTCCCCC 
AGTGCGCTCA CACTTCAGCA TGGTGACCTT GTACAAGTGG AACAATGTGG AGACAGTGGA 
CAGTGGTTAG TAAAAGAAGT TGTTTCCAAG AAAACTGCAT GGGTACCTTC CAACATGTTA 
ATTCCTGCCA GGAGCGATAT TAAGAACATC TGCCAAGAGG ATAACAGCAC AGCTTACAGT 
GAAGCATGTC AAATATCACT GGCATCAACA AGCATGAAAG AGAAGCAGAA TGAAAGGACA 
CGAAGCTTGG TGGCTGAGCA GAAGGCAGTG AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_012963327.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012818781.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa58344
Clone ID Related Accession (Same CDS sequence)	XM_012963327.3
Accession Version	XM_012963327.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012963327.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGAATCTC AGGAGAAAGA ATCTACTCGG 
CGATTCTCCC TAGCTTCTTC TGTGAGCTCA ACATCTATTA AAGTTCCTAC TCGTACAAAA 
GGTCCACTTG GCCCTGATGT GAAAGCAAAG CGACATGAGA TAAAGAGTGA CCCAACCCCC 
CTTGGTTTCG AAGGCTGGCC GAGACACATG AACTCTATGG AAACTTTCCA AGACTTTTCA 
GCTATCCCAT CCAGCAATGA TGAACTTTCC AATTCTTCAG ATGTTGATGA GGATATAACA 
AATAGCAAAA GATCCAATCT GTTTCGTGTG GAAGGATGTT TGGAAGTATG TTCCCCCAGT 
GCGCTCACAC TTCAGCATGG TGACCTTGTA CAAGTGGAAC AATGTGGAGA CAGTGGACAG 
TGGTTAGTAA AAGAAGTTGT TTCCAAGAAA ACTGCATGGG TACCTTCCAA CATGTTAATT 
CCTGCCAGGA GCGATATTAA GAACATCTGC CAAGAGGATA ACAGCACAGC TTACAGTGAA 
GCATGTCAAA TATCACTGGC ATCAACAAGC ATGAAAGAGA AGCAGAATGA AAGGACACGA 
AGCTTGGTGG CTGAGCAGAA GGCAGTGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012818781.1
CDS	75..3947
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_012963327.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012963327.3

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGAATCTC AGGAGAAAGA ATCTACTCGG 
CGATTCTCCC TAGCTTCTTC TGTGAGCTCA ACATCTATTA AAGTTCCTAC TCGTACAAAA 
GGTCCACTTG GCCCTGATGT GAAAGCAAAG CGACATGAGA TAAAGAGTGA CCCAACCCCC 
CTTGGTTTCG AAGGCTGGCC GAGACACATG AACTCTATGG AAACTTTCCA AGACTTTTCA 
GCTATCCCAT CCAGCAATGA TGAACTTTCC AATTCTTCAG ATGTTGATGA GGATATAACA 
AATAGCAAAA GATCCAATCT GTTTCGTGTG GAAGGATGTT TGGAAGTATG TTCCCCCAGT 
GCGCTCACAC TTCAGCATGG TGACCTTGTA CAAGTGGAAC AATGTGGAGA CAGTGGACAG 
TGGTTAGTAA AAGAAGTTGT TTCCAAGAAA ACTGCATGGG TACCTTCCAA CATGTTAATT 
CCTGCCAGGA GCGATATTAA GAACATCTGC CAAGAGGATA ACAGCACAGC TTACAGTGAA 
GCATGTCAAA TATCACTGGC ATCAACAAGC ATGAAAGAGA AGCAGAATGA AAGGACACGA 
AGCTTGGTGG CTGAGCAGAA GGCAGTGAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_031902050.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757910.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X3, mRNA.

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CloneID	OXa58345
Clone ID Related Accession (Same CDS sequence)	XM_031902050.1
Accession Version	XM_031902050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGAT TGTAATGATA 
AACTCAGTTT CTGATCTTCA TGGATACATT GATAAAAGCC AGCTAACCAC TGAGCTGGGA 
GGTACCTTGG AATACAGCCA TAGTCAATGG ATACATCATA GAACAGCCAT TGAGAGCTTT 
GCTCAAGTTG TGAAAACGAC AGCACAGAAC CTGCAGACCT TTGGAACAAG CCTTGCAGAG 
ACTGAGCTTC CAAATGATGT ACTGTGCACA GAGGAGCTGC TGCAGTCCCA CACAGAGCAA 
CAGATCAGTC TAAAAAATGA CCTGAACTTG GCAATGCAAC AAGGTGGCAC GCTGCTCTCA 
TGCATAAAGG AACCAGTATC AAAAGACCCA AGCTACAAAC TAAATCCAGA TGAGGTTGAG 
AACATGGCTA CAGTTGAAAG ACTTCTGGCT CAGCTAGATG AAACAGAAAA AGCTTTTGGT 
CACTTCTGGA GTCGGCATCA ACTAAAGTTA GAACAATGTT TGCAACTGCG ACACTTTGAA 
CATGATTTCA GAGAGTTGAA GCTAACTTTG GATGGACTGA TGGAGATCCA AGCTGCATTT 
GCAGACATTG GAGACAGTGT GTCACGGGTG GAATACCTTA TTAAGGAGCT AAAAAAGCTG 
GAAGAGAAAG GACAGGGAAT GTTGGAGAAA GCCCAGCTGC ATGCTCTTCA CGGTGACCAG 
CTTATCCAAA ACAACCACTA TGCTGTGGAC TCTATTAGAC CAAAATGCAT AGAACTCAGG 
CGAATCTGTG ATGACTTTAC CAATGAGACA AAGAAAAAGT ATGACATATT GGCAAAATCA 
CTGGAGCTGC ACCAGCAACT TGACGAGGCC AACAAGTGGT GTGAAGATGG AATCTACCTT 
TTGGCTTCCC AAGCTGTGGA TAAGTGCCAG TCACAGGAAG GTGCAGAGTC TGCTTTACGG 
GACATAGAAC TGTTCATTGA AAGCTCAAGA GGACACCAGC TTTCCAATCC AACAGATTTC 
TACAATAAAT ATGATGCAAT TCTCACTCTT GAAATCAAAG CAAATGTTCA GAAAGTTTTG 
CAGAAGGTGG CTGATGTTCA GGAGATGTTT AATAAGAGAC AAGCAAGCCT CAAAAAGCTG 
GCAGCCAAAC AGACAAGACC TATCCAGCCT GTGGCCCCAC ATCCAGAGTC CTCACCAAAG 
AGGTCATCTC CAAAGACTGT ACGACCCACC TCACTGTCCA CAACCCGAAG AGCAGCTGAT 
GTGCCATGCG TGCCTCCCAA GTCTCTCTCT GAAGCAGACC TGTCCAGGAA AAGGAACAAT 
AAGAAAACCA AAGCAGGAAT AAAGATTGAA GTAATGCATG AAGCTAGTCA GGGTGGCACA 
AGCCGCACAT TGGTGATGAG TGAGAGTGAA GAAAACCTCT CTTCAAGAAA GAGCCACATC 
ATAAATGAGT TAATTGAAAC AGAACGTGTC TATGTTGAAG AGCTTCAAAG TATCCTAGAG 
GGTTATGCAT CATCTCTGGA AAATCCTGAA ATGATTGGTT TGATACCAGT GGCATTGCAA 
AACAATAAGG AAGTTCTCTT TGGAAACCTT TCAGAAATTT ATGAATTTCA CAAAAGAATT 
TTCCTCAAAG AATTAGAGAA CTGTATTGAA AATGCTGATT TGCTTGGAAC ATGTTTCTTA 
AAAAGGAAAG AGGACCTTCA GATTTATGAA AAGTACTGCC AAAATAAACC AAGGTCGGAA 
GCAATCTGGA GACAGTGTGC AGAGAGCATT TTTTTTCAGG AATGCCAGCG TAGACTGGAT 
CATAAGCTGT CTTTAGATGC TTATCTTCTT AAGCCTGTCC AAAGGATCAC AAAATATCAG 
CTGCTTTTAA AGGAAATGTT GAAATGCAGT AAGAATTCTG AAGGGACAGC TGAACTAGAA 
GAAGCACTTG CCACAGTGTT GGACATAATT AAGTCAGTGA ATGACTCAAT GCACCAAATA 
GCTATAACAG GATATGAGGG GGATCTGAAC GACCTTGGTA AGCTGCTTAT GCAGGGCTCA 
TTTAATGTGT GGACTGATCA CAAGAAAGGG CACAACAAGG TAAAAGACTT GGCCCGATTC 
AAACCCATGC AACGGCATCT CTTTTTATAT GCTAAGGCCC TACTGTTTTG CAAGAAGAGG 
GAAGAGAACA GTGAAGGACA TGAAAAATCT CAGTCTTACA GTTTCAAGCA TATTTTAAAG 
ATGAGTTCAG TTGGCATCAC TGAAAATGTT AAAGGGGACA ACAAGAAGTT TGAAGTATGG 
TACAATGGCA GAGAAGAAGT CTACATTATT CAGGCATCAA CTATTGAGCT GAAAAACCTT 
TGGGTTTCTG AAATCAGAAA AGTCCTTACA GGCCAACTGG AAGCTTGCAG AGAAGCAAGC 
CAATTACATC AAAGGATCAC AGAGAGCGTA TACCACGCAC CAATGAACTC CTCATTTATT 
GCCAGGTCCA ATAAGACTTC AGGTAACAGC CAGAACAAAG GAGAATTGCC AAGTCTTGAA 
GTGTCCAATA AGAAGAACAG GACTTCTAGT CCAACTGGAA GACAAAAGAA AGCTGATAAT 
CCTGCCAGTC ATAATCTTGA GCGCTCAGCT CTTGCTAGAA AAAGATTCAC ATTACAGGGT 
CTTTCCAATC GGAGAGCTCC ACACAAAGCA GAATCTCAGG AGAAAGAATC TACTCGGCGA 
TTCTCCCTAG CTTCTTCTGT GAGCTCAACA TCTATTAAAG TTCCTACTCG TACAAAAGGT 
CCACTTGGCC CTGATGTGAA AGCAAAGCGA CATGAGATAA AGAGTGACCC AACCCCCCTT 
GGTTTCGAAG GCTGGCCGAG ACACATGAAC TCTATGGAAA CTTTCCAAGA CTTTTCAGCT 
ATCCCATCCA GCAATGATGA ACTTTCCAAT TCTTCAGATG TTGATGAGGA TATAACAAAT 
AGCAAAAGAT CCAATCTGTT TCGTGTGGAA GGATGTTTGG AAGTATGTTC CCCCAGTGCG 
CTCACACTTC AGCATGGTGA CCTTGTACAA GTGGAACAAT GTGGAGACAG TGGACAGTGG 
TTAGTAAAAG AAGTTGTTTC CAAGAAAACT GCATGGGTAC CTTCCAACAT GTTAATTCCT 
GCCAGGAGCG ATATTAAGAA CATCTGCCAA GAGGATAACA GCACAGCTTA CAGTGAAGCA 
TGTCAAATAT CACTGGCATC AACAAGCATG AAAGAGAAGC AGAATGAAAG GACACGAAGC 
TTGGTGGCTG AGCAGAAGGC AGTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757910.1
CDS	76..3945
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKAESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_031902050.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902050.1

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGAT TGTAATGATA 
AACTCAGTTT CTGATCTTCA TGGATACATT GATAAAAGCC AGCTAACCAC TGAGCTGGGA 
GGTACCTTGG AATACAGCCA TAGTCAATGG ATACATCATA GAACAGCCAT TGAGAGCTTT 
GCTCAAGTTG TGAAAACGAC AGCACAGAAC CTGCAGACCT TTGGAACAAG CCTTGCAGAG 
ACTGAGCTTC CAAATGATGT ACTGTGCACA GAGGAGCTGC TGCAGTCCCA CACAGAGCAA 
CAGATCAGTC TAAAAAATGA CCTGAACTTG GCAATGCAAC AAGGTGGCAC GCTGCTCTCA 
TGCATAAAGG AACCAGTATC AAAAGACCCA AGCTACAAAC TAAATCCAGA TGAGGTTGAG 
AACATGGCTA CAGTTGAAAG ACTTCTGGCT CAGCTAGATG AAACAGAAAA AGCTTTTGGT 
CACTTCTGGA GTCGGCATCA ACTAAAGTTA GAACAATGTT TGCAACTGCG ACACTTTGAA 
CATGATTTCA GAGAGTTGAA GCTAACTTTG GATGGACTGA TGGAGATCCA AGCTGCATTT 
GCAGACATTG GAGACAGTGT GTCACGGGTG GAATACCTTA TTAAGGAGCT AAAAAAGCTG 
GAAGAGAAAG GACAGGGAAT GTTGGAGAAA GCCCAGCTGC ATGCTCTTCA CGGTGACCAG 
CTTATCCAAA ACAACCACTA TGCTGTGGAC TCTATTAGAC CAAAATGCAT AGAACTCAGG 
CGAATCTGTG ATGACTTTAC CAATGAGACA AAGAAAAAGT ATGACATATT GGCAAAATCA 
CTGGAGCTGC ACCAGCAACT TGACGAGGCC AACAAGTGGT GTGAAGATGG AATCTACCTT 
TTGGCTTCCC AAGCTGTGGA TAAGTGCCAG TCACAGGAAG GTGCAGAGTC TGCTTTACGG 
GACATAGAAC TGTTCATTGA AAGCTCAAGA GGACACCAGC TTTCCAATCC AACAGATTTC 
TACAATAAAT ATGATGCAAT TCTCACTCTT GAAATCAAAG CAAATGTTCA GAAAGTTTTG 
CAGAAGGTGG CTGATGTTCA GGAGATGTTT AATAAGAGAC AAGCAAGCCT CAAAAAGCTG 
GCAGCCAAAC AGACAAGACC TATCCAGCCT GTGGCCCCAC ATCCAGAGTC CTCACCAAAG 
AGGTCATCTC CAAAGACTGT ACGACCCACC TCACTGTCCA CAACCCGAAG AGCAGCTGAT 
GTGCCATGCG TGCCTCCCAA GTCTCTCTCT GAAGCAGACC TGTCCAGGAA AAGGAACAAT 
AAGAAAACCA AAGCAGGAAT AAAGATTGAA GTAATGCATG AAGCTAGTCA GGGTGGCACA 
AGCCGCACAT TGGTGATGAG TGAGAGTGAA GAAAACCTCT CTTCAAGAAA GAGCCACATC 
ATAAATGAGT TAATTGAAAC AGAACGTGTC TATGTTGAAG AGCTTCAAAG TATCCTAGAG 
GGTTATGCAT CATCTCTGGA AAATCCTGAA ATGATTGGTT TGATACCAGT GGCATTGCAA 
AACAATAAGG AAGTTCTCTT TGGAAACCTT TCAGAAATTT ATGAATTTCA CAAAAGAATT 
TTCCTCAAAG AATTAGAGAA CTGTATTGAA AATGCTGATT TGCTTGGAAC ATGTTTCTTA 
AAAAGGAAAG AGGACCTTCA GATTTATGAA AAGTACTGCC AAAATAAACC AAGGTCGGAA 
GCAATCTGGA GACAGTGTGC AGAGAGCATT TTTTTTCAGG AATGCCAGCG TAGACTGGAT 
CATAAGCTGT CTTTAGATGC TTATCTTCTT AAGCCTGTCC AAAGGATCAC AAAATATCAG 
CTGCTTTTAA AGGAAATGTT GAAATGCAGT AAGAATTCTG AAGGGACAGC TGAACTAGAA 
GAAGCACTTG CCACAGTGTT GGACATAATT AAGTCAGTGA ATGACTCAAT GCACCAAATA 
GCTATAACAG GATATGAGGG GGATCTGAAC GACCTTGGTA AGCTGCTTAT GCAGGGCTCA 
TTTAATGTGT GGACTGATCA CAAGAAAGGG CACAACAAGG TAAAAGACTT GGCCCGATTC 
AAACCCATGC AACGGCATCT CTTTTTATAT GCTAAGGCCC TACTGTTTTG CAAGAAGAGG 
GAAGAGAACA GTGAAGGACA TGAAAAATCT CAGTCTTACA GTTTCAAGCA TATTTTAAAG 
ATGAGTTCAG TTGGCATCAC TGAAAATGTT AAAGGGGACA ACAAGAAGTT TGAAGTATGG 
TACAATGGCA GAGAAGAAGT CTACATTATT CAGGCATCAA CTATTGAGCT GAAAAACCTT 
TGGGTTTCTG AAATCAGAAA AGTCCTTACA GGCCAACTGG AAGCTTGCAG AGAAGCAAGC 
CAATTACATC AAAGGATCAC AGAGAGCGTA TACCACGCAC CAATGAACTC CTCATTTATT 
GCCAGGTCCA ATAAGACTTC AGGTAACAGC CAGAACAAAG GAGAATTGCC AAGTCTTGAA 
GTGTCCAATA AGAAGAACAG GACTTCTAGT CCAACTGGAA GACAAAAGAA AGCTGATAAT 
CCTGCCAGTC ATAATCTTGA GCGCTCAGCT CTTGCTAGAA AAAGATTCAC ATTACAGGGT 
CTTTCCAATC GGAGAGCTCC ACACAAAGCA GAATCTCAGG AGAAAGAATC TACTCGGCGA 
TTCTCCCTAG CTTCTTCTGT GAGCTCAACA TCTATTAAAG TTCCTACTCG TACAAAAGGT 
CCACTTGGCC CTGATGTGAA AGCAAAGCGA CATGAGATAA AGAGTGACCC AACCCCCCTT 
GGTTTCGAAG GCTGGCCGAG ACACATGAAC TCTATGGAAA CTTTCCAAGA CTTTTCAGCT 
ATCCCATCCA GCAATGATGA ACTTTCCAAT TCTTCAGATG TTGATGAGGA TATAACAAAT 
AGCAAAAGAT CCAATCTGTT TCGTGTGGAA GGATGTTTGG AAGTATGTTC CCCCAGTGCG 
CTCACACTTC AGCATGGTGA CCTTGTACAA GTGGAACAAT GTGGAGACAG TGGACAGTGG 
TTAGTAAAAG AAGTTGTTTC CAAGAAAACT GCATGGGTAC CTTCCAACAT GTTAATTCCT 
GCCAGGAGCG ATATTAAGAA CATCTGCCAA GAGGATAACA GCACAGCTTA CAGTGAAGCA 
TGTCAAATAT CACTGGCATC AACAAGCATG AAAGAGAAGC AGAATGAAAG GACACGAAGC 
TTGGTGGCTG AGCAGAAGGC AGTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_002937839.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002937885.2 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa58346
Clone ID Related Accession (Same CDS sequence)	XM_002937839.5
Accession Version	XM_002937839.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002937839.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGAA 
TCTCAGGAGA AAGAATCTAC TCGGCGATTC TCCCTAGCTT CTTCTGTGAG CTCAACATCT 
ATTAAAGTTC CTACTCGTAC AAAAGGTCCA CTTGGCCCTG ATGTGAAAGC AAAGCGACAT 
GAGATAAAGA GTGACCCAAC CCCCCTTGGT TTCGAAGGCT GGCCGAGACA CATGAACTCT 
ATGGAAACTT TCCAAGACTT TTCAGCTATC CCATCCAGCA ATGATGAACT TTCCAATTCT 
TCAGATGTTG ATGAGGATAT AACAAATAGC AAAAGATCCA ATCTGTTTCG TGTGGAAGGA 
TGTTTGGAAG TATGTTCCCC CAGTGCGCTC ACACTTCAGC ATGGTGACCT TGTACAAGTG 
GAACAATGTG GAGACAGTGG ACAGTGGTTA GTAAAAGAAG TTGTTTCCAA GAAAACTGCA 
TGGGTACCTT CCAACATGTT AATTCCTGCC AGGAGCGATA TTAAGAACAT CTGCCAAGAG 
GATAACAGCA CAGCTTACAG TGAAGCATGT CAAATATCAC TGGCATCAAC AAGCATGAAA 
GAGAAGCAGA ATGAAAGGAC ACGAAGCTTG GTGGCTGAGC AGAAGGCAGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002937885.2
CDS	75..3851
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_002937839.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002937839.5

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGAA 
TCTCAGGAGA AAGAATCTAC TCGGCGATTC TCCCTAGCTT CTTCTGTGAG CTCAACATCT 
ATTAAAGTTC CTACTCGTAC AAAAGGTCCA CTTGGCCCTG ATGTGAAAGC AAAGCGACAT 
GAGATAAAGA GTGACCCAAC CCCCCTTGGT TTCGAAGGCT GGCCGAGACA CATGAACTCT 
ATGGAAACTT TCCAAGACTT TTCAGCTATC CCATCCAGCA ATGATGAACT TTCCAATTCT 
TCAGATGTTG ATGAGGATAT AACAAATAGC AAAAGATCCA ATCTGTTTCG TGTGGAAGGA 
TGTTTGGAAG TATGTTCCCC CAGTGCGCTC ACACTTCAGC ATGGTGACCT TGTACAAGTG 
GAACAATGTG GAGACAGTGG ACAGTGGTTA GTAAAAGAAG TTGTTTCCAA GAAAACTGCA 
TGGGTACCTT CCAACATGTT AATTCCTGCC AGGAGCGATA TTAAGAACAT CTGCCAAGAG 
GATAACAGCA CAGCTTACAG TGAAGCATGT CAAATATCAC TGGCATCAAC AAGCATGAAA 
GAGAAGCAGA ATGAAAGGAC ACGAAGCTTG GTGGCTGAGC AGAAGGCAGT GAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_031902051.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757911.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa58347
Clone ID Related Accession (Same CDS sequence)	XM_031902051.1
Accession Version	XM_031902051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGGT 
CCACTTGGCC CTGATGTGAA AGCAAAGCGA CATGAGATAA AGAGTGACCC AACCCCCCTT 
GGTTTCGAAG GCTGGCCGAG ACACATGAAC TCTATGGAAA CTTTCCAAGA CTTTTCAGCT 
ATCCCATCCA GCAATGATGA ACTTTCCAAT TCTTCAGATG TTGATGAGGA TATAACAAAT 
AGCAAAAGAT CCAATCTGTT TCGTGTGGAA GGATGTTTGG AAGTATGTTC CCCCAGTGCG 
CTCACACTTC AGCATGGTGA CCTTGTACAA GTGGAACAAT GTGGAGACAG TGGACAGTGG 
TTAGTAAAAG AAGTTGTTTC CAAGAAAACT GCATGGGTAC CTTCCAACAT GTTAATTCCT 
GCCAGGAGCG ATATTAAGAA CATCTGCCAA GAGGATAACA GCACAGCTTA CAGTGAAGCA 
TGTCAAATAT CACTGGCATC AACAAGCATG AAAGAGAAGC AGAATGAAAG GACACGAAGC 
TTGGTGGCTG AGCAGAAGGC AGTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757911.1
CDS	75..3764
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKRSNLFRVEGCLEVCSPSALTLQHGDLVQVEQCGDSGQWLVKEVVSKKTAWVPSNMLIPARSDIKNICQEDNSTAYSEACQISLASTSMKEKQNERTRSLVAEQKAVS

Target ORF information:

RefSeq Version	XM_031902051.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902051.1

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGGT 
CCACTTGGCC CTGATGTGAA AGCAAAGCGA CATGAGATAA AGAGTGACCC AACCCCCCTT 
GGTTTCGAAG GCTGGCCGAG ACACATGAAC TCTATGGAAA CTTTCCAAGA CTTTTCAGCT 
ATCCCATCCA GCAATGATGA ACTTTCCAAT TCTTCAGATG TTGATGAGGA TATAACAAAT 
AGCAAAAGAT CCAATCTGTT TCGTGTGGAA GGATGTTTGG AAGTATGTTC CCCCAGTGCG 
CTCACACTTC AGCATGGTGA CCTTGTACAA GTGGAACAAT GTGGAGACAG TGGACAGTGG 
TTAGTAAAAG AAGTTGTTTC CAAGAAAACT GCATGGGTAC CTTCCAACAT GTTAATTCCT 
GCCAGGAGCG ATATTAAGAA CATCTGCCAA GAGGATAACA GCACAGCTTA CAGTGAAGCA 
TGTCAAATAT CACTGGCATC AACAAGCATG AAAGAGAAGC AGAATGAAAG GACACGAAGC 
TTGGTGGCTG AGCAGAAGGC AGTGAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mcf2l.2 ( XM_031902052.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757912.1 Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X6, mRNA.

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CloneID	OXa58348
Clone ID Related Accession (Same CDS sequence)	XM_031902052.1
Accession Version	XM_031902052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	guanine nucleotide exchange factor DBS isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030681.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGCAGAAT CTCAGGAGAA AGAATCTACT 
CGGCGATTCT CCCTAGCTTC TTCTGTGAGC TCAACATCTA TTAAAGTTCC TACTCGTACA 
AAAGGTCCAC TTGGCCCTGA TGTGAAAGCA AAGCGACATG AGATAAAGAG TGACCCAACC 
CCCCTTGGTT TCGAAGGCTG GCCGAGACAC ATGAACTCTA TGGAAACTTT CCAAGACTTT 
TCAGCTATCC CATCCAGCAA TGATGAACTT TCCAATTCTT CAGATGTTGA TGAGGATATA 
ACAAATAGCA AAGTTAGTAA AAGAAGTTGT TTCCAAGAAA ACTGCATGGG TACCTTCCAA 
CATGTTAATT CCTGCCAGGA GCGATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757912.1
CDS	80..3709
Translation	MLSTGAALLMPDRSQCVLPIVTGGCQAAELGVALPRFVPHIHRQLGRAPGISPWEMLFWLKEEEMSHQELTQRLSTVITHVDEIMQQEIRPLLAVDIIEQLHKQFASLTGGRGKDGAPIITFPEYPGFTEVPDEDFLNVVTYLTSIPSLEAASIGFIIVIDRRKDKWSSVKASLTRIAGAFPGNLQLVFILRPSRFIQRTISDIGIKLYRDDFKMKVPIVMINSVSDLHGYIDKSQLTTELGGTLEYSHSQWIHHRTAIESFAQVVKTTAQNLQTFGTSLAETELPNDVLCTEELLQSHTEQQISLKNDLNLAMQQGGTLLSCIKEPVSKDPSYKLNPDEVENMATVERLLAQLDETEKAFGHFWSRHQLKLEQCLQLRHFEHDFRELKLTLDGLMEIQAAFADIGDSVSRVEYLIKELKKLEEKGQGMLEKAQLHALHGDQLIQNNHYAVDSIRPKCIELRRICDDFTNETKKKYDILAKSLELHQQLDEANKWCEDGIYLLASQAVDKCQSQEGAESALRDIELFIESSRGHQLSNPTDFYNKYDAILTLEIKANVQKVLQKVADVQEMFNKRQASLKKLAAKQTRPIQPVAPHPESSPKRSSPKTVRPTSLSTTRRAADVPCVPPKSLSEADLSRKRNNKKTKAGIKIEVMHEASQGGTSRTLVMSESEENLSSRKSHIINELIETERVYVEELQSILEGYASSLENPEMIGLIPVALQNNKEVLFGNLSEIYEFHKRIFLKELENCIENADLLGTCFLKRKEDLQIYEKYCQNKPRSEAIWRQCAESIFFQECQRRLDHKLSLDAYLLKPVQRITKYQLLLKEMLKCSKNSEGTAELEEALATVLDIIKSVNDSMHQIAITGYEGDLNDLGKLLMQGSFNVWTDHKKGHNKVKDLARFKPMQRHLFLYAKALLFCKKREENSEGHEKSQSYSFKHILKMSSVGITENVKGDNKKFEVWYNGREEVYIIQASTIELKNLWVSEIRKVLTGQLEACREASQLHQRITESVYHAPMNSSFIARSNKTSGNSQNKGELPSLEVSNKKNRTSSPTGRQKKADNPASHNLERSALARKRFTLQGLSNRRAPHKAESQEKESTRRFSLASSVSSTSIKVPTRTKGPLGPDVKAKRHEIKSDPTPLGFEGWPRHMNSMETFQDFSAIPSSNDELSNSSDVDEDITNSKVSKRSCFQENCMGTFQHVNSCQERY

Target ORF information:

RefSeq Version	XM_031902052.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis MCF.2 cell line derived transforming sequence like (mcf2l.2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031902052.1

ORF Insert Sequence:

ATGCTAAGCA CCGGGGCTGC ATTGCTCATG CCTGACCGCA GCCAGTGTGT GTTGCCAATA 
GTGACAGGTG GATGTCAGGC TGCTGAGCTG GGAGTGGCAC TGCCTCGCTT TGTACCCCAC 
ATACACCGGC AATTAGGGCG GGCACCCGGC ATCTCTCCTT GGGAAATGCT TTTTTGGCTT 
AAAGAAGAAG AGATGTCACA TCAGGAATTA ACGCAAAGGC TCTCCACAGT CATCACTCAT 
GTCGATGAAA TCATGCAGCA AGAGATAAGG CCACTGCTGG CTGTGGACAT CATAGAGCAA 
CTTCATAAAC AGTTTGCTAG CTTGACAGGA GGAAGAGGAA AGGATGGAGC ACCTATAATA 
ACATTTCCAG AATACCCAGG CTTTACTGAA GTCCCTGATG AGGATTTCCT GAATGTTGTA 
ACATATTTAA CAAGCATTCC AAGCCTTGAA GCTGCCAGTA TTGGGTTCAT CATTGTGATC 
GACAGGAGGA AAGATAAATG GAGCTCAGTG AAAGCATCCC TGACACGAAT AGCAGGAGCA 
TTCCCAGGAA ACCTGCAACT TGTTTTCATT CTGCGTCCAT CCCGTTTCAT CCAGAGGACC 
ATCTCTGATA TTGGCATCAA GCTCTACCGA GATGACTTTA AAATGAAGGT ACCGATTGTA 
ATGATAAACT CAGTTTCTGA TCTTCATGGA TACATTGATA AAAGCCAGCT AACCACTGAG 
CTGGGAGGTA CCTTGGAATA CAGCCATAGT CAATGGATAC ATCATAGAAC AGCCATTGAG 
AGCTTTGCTC AAGTTGTGAA AACGACAGCA CAGAACCTGC AGACCTTTGG AACAAGCCTT 
GCAGAGACTG AGCTTCCAAA TGATGTACTG TGCACAGAGG AGCTGCTGCA GTCCCACACA 
GAGCAACAGA TCAGTCTAAA AAATGACCTG AACTTGGCAA TGCAACAAGG TGGCACGCTG 
CTCTCATGCA TAAAGGAACC AGTATCAAAA GACCCAAGCT ACAAACTAAA TCCAGATGAG 
GTTGAGAACA TGGCTACAGT TGAAAGACTT CTGGCTCAGC TAGATGAAAC AGAAAAAGCT 
TTTGGTCACT TCTGGAGTCG GCATCAACTA AAGTTAGAAC AATGTTTGCA ACTGCGACAC 
TTTGAACATG ATTTCAGAGA GTTGAAGCTA ACTTTGGATG GACTGATGGA GATCCAAGCT 
GCATTTGCAG ACATTGGAGA CAGTGTGTCA CGGGTGGAAT ACCTTATTAA GGAGCTAAAA 
AAGCTGGAAG AGAAAGGACA GGGAATGTTG GAGAAAGCCC AGCTGCATGC TCTTCACGGT 
GACCAGCTTA TCCAAAACAA CCACTATGCT GTGGACTCTA TTAGACCAAA ATGCATAGAA 
CTCAGGCGAA TCTGTGATGA CTTTACCAAT GAGACAAAGA AAAAGTATGA CATATTGGCA 
AAATCACTGG AGCTGCACCA GCAACTTGAC GAGGCCAACA AGTGGTGTGA AGATGGAATC 
TACCTTTTGG CTTCCCAAGC TGTGGATAAG TGCCAGTCAC AGGAAGGTGC AGAGTCTGCT 
TTACGGGACA TAGAACTGTT CATTGAAAGC TCAAGAGGAC ACCAGCTTTC CAATCCAACA 
GATTTCTACA ATAAATATGA TGCAATTCTC ACTCTTGAAA TCAAAGCAAA TGTTCAGAAA 
GTTTTGCAGA AGGTGGCTGA TGTTCAGGAG ATGTTTAATA AGAGACAAGC AAGCCTCAAA 
AAGCTGGCAG CCAAACAGAC AAGACCTATC CAGCCTGTGG CCCCACATCC AGAGTCCTCA 
CCAAAGAGGT CATCTCCAAA GACTGTACGA CCCACCTCAC TGTCCACAAC CCGAAGAGCA 
GCTGATGTGC CATGCGTGCC TCCCAAGTCT CTCTCTGAAG CAGACCTGTC CAGGAAAAGG 
AACAATAAGA AAACCAAAGC AGGAATAAAG ATTGAAGTAA TGCATGAAGC TAGTCAGGGT 
GGCACAAGCC GCACATTGGT GATGAGTGAG AGTGAAGAAA ACCTCTCTTC AAGAAAGAGC 
CACATCATAA ATGAGTTAAT TGAAACAGAA CGTGTCTATG TTGAAGAGCT TCAAAGTATC 
CTAGAGGGTT ATGCATCATC TCTGGAAAAT CCTGAAATGA TTGGTTTGAT ACCAGTGGCA 
TTGCAAAACA ATAAGGAAGT TCTCTTTGGA AACCTTTCAG AAATTTATGA ATTTCACAAA 
AGAATTTTCC TCAAAGAATT AGAGAACTGT ATTGAAAATG CTGATTTGCT TGGAACATGT 
TTCTTAAAAA GGAAAGAGGA CCTTCAGATT TATGAAAAGT ACTGCCAAAA TAAACCAAGG 
TCGGAAGCAA TCTGGAGACA GTGTGCAGAG AGCATTTTTT TTCAGGAATG CCAGCGTAGA 
CTGGATCATA AGCTGTCTTT AGATGCTTAT CTTCTTAAGC CTGTCCAAAG GATCACAAAA 
TATCAGCTGC TTTTAAAGGA AATGTTGAAA TGCAGTAAGA ATTCTGAAGG GACAGCTGAA 
CTAGAAGAAG CACTTGCCAC AGTGTTGGAC ATAATTAAGT CAGTGAATGA CTCAATGCAC 
CAAATAGCTA TAACAGGATA TGAGGGGGAT CTGAACGACC TTGGTAAGCT GCTTATGCAG 
GGCTCATTTA ATGTGTGGAC TGATCACAAG AAAGGGCACA ACAAGGTAAA AGACTTGGCC 
CGATTCAAAC CCATGCAACG GCATCTCTTT TTATATGCTA AGGCCCTACT GTTTTGCAAG 
AAGAGGGAAG AGAACAGTGA AGGACATGAA AAATCTCAGT CTTACAGTTT CAAGCATATT 
TTAAAGATGA GTTCAGTTGG CATCACTGAA AATGTTAAAG GGGACAACAA GAAGTTTGAA 
GTATGGTACA ATGGCAGAGA AGAAGTCTAC ATTATTCAGG CATCAACTAT TGAGCTGAAA 
AACCTTTGGG TTTCTGAAAT CAGAAAAGTC CTTACAGGCC AACTGGAAGC TTGCAGAGAA 
GCAAGCCAAT TACATCAAAG GATCACAGAG AGCGTATACC ACGCACCAAT GAACTCCTCA 
TTTATTGCCA GGTCCAATAA GACTTCAGGT AACAGCCAGA ACAAAGGAGA ATTGCCAAGT 
CTTGAAGTGT CCAATAAGAA GAACAGGACT TCTAGTCCAA CTGGAAGACA AAAGAAAGCT 
GATAATCCTG CCAGTCATAA TCTTGAGCGC TCAGCTCTTG CTAGAAAAAG ATTCACATTA 
CAGGGTCTTT CCAATCGGAG AGCTCCACAC AAAGCAGAAT CTCAGGAGAA AGAATCTACT 
CGGCGATTCT CCCTAGCTTC TTCTGTGAGC TCAACATCTA TTAAAGTTCC TACTCGTACA 
AAAGGTCCAC TTGGCCCTGA TGTGAAAGCA AAGCGACATG AGATAAAGAG TGACCCAACC 
CCCCTTGGTT TCGAAGGCTG GCCGAGACAC ATGAACTCTA TGGAAACTTT CCAAGACTTT 
TCAGCTATCC CATCCAGCAA TGATGAACTT TCCAATTCTT CAGATGTTGA TGAGGATATA 
ACAAATAGCA AAGTTAGTAA AAGAAGTTGT TTCCAAGAAA ACTGCATGGG TACCTTCCAA 
CATGTTAATT CCTGCCAGGA GCGATATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mcf2l.2
Entrez Gene ID	100380200
Full Name	MCF.2 cell line derived transforming sequence like
Synonyms	arhgef14,dbs,ost
General protein information	Preferred Names MCF.2 cell line derived transforming sequence like Names guanine nucleotide exchange factor DBS
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Danio rerio (zebrafish)	mcf2l2	XP_001921389.4
Xenopus tropicalis (tropical clawed frog)	mcf2l.2	XP_002937885.2
Gallus gallus (chicken)	MCF2L2	XP_004943496.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007723	XP_564500.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mcf2l.2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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