mcf2l2 cDNA ORF clone, Danio rerio(zebrafish)

The following mcf2l2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcf2l2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa54315 XM_017358288.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa147961 XM_021479735.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147962 XM_021479736.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147963 XM_021479737.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147964 XM_021479738.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147965 XM_021479739.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa147966 XM_021479740.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa54310 XM_017358283.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54311 XM_017358284.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54312 XM_017358285.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54313 XM_017358286.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa54314 XM_017358287.1
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa48787 XM_009305853.3
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa48788 XM_009305854.3
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa48789 XM_009305855.3
Latest version!
Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa48787 XM_009305853.2 Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa48788 XM_009305854.2 Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa48789 XM_009305855.2 Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa54315
    Clone ID Related Accession (Same CDS sequence) XM_017358288.1
    Accession Version XM_017358288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGATCT CGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213777.1
    CDS322..3318
    Translation

    Target ORF information:

    RefSeq Version XM_017358288.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGATCT CGGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147961
    Clone ID Related Accession (Same CDS sequence) XM_021479735.1
    Accession Version XM_021479735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GCTGACACTT CATCCAGCCT CGGCTCAGAG CGAGCGGCTC TTCCTAGAAA GCGTTTCACC
    TTACAGGGCC TGTCAAACCG CAGATCTCTG CCCGCAGAAC CCGCACCTAA GGAGACAGAA
    GTCACTCGCC GCTTTTCTTT AGCCTCAACT GTCGGCTCTG CCACTGCTCC ACGCACTAAA
    GCTCCTCTCT CCTCTAGCCT TAAGAGTAAG AGGCATCAGA TCAAGAGCGA CCCTACACCA
    TTTGGCTATG AAGGTGCTAC CAAGACACGC GGTTTTAAAA AAGAAACCAT GCGAGATTCC
    CTTCCAGCCG AGAGAAAAAG TAAAGGTAGC GGCGGTTCGA AGCCCGCTGT GCCGAGGTCT
    GCGTCTCAGC CAGGATGGAG TCAACGGCGT CTGCCTTCAA TGGACGCTGA GGATTTCGAG
    ACCATTCCGT CTAGTGCTGA AGAGCTGTCT ATTTCCTCTG ATGGAGAGGA TGAAGGACAC
    AACAAAAACG GGGATAATGA CCGCTATCAG GTGCAGTTGA CTTACGAGTC TTGCACTGCT
    CAGGATCTTT CTGTAGAAGC TGGAGATTTT GTACAATTCC TGGAAGAGTC AGAGAACGGC
    CTCTGGCTAG TCAAGAACCT TAGAACTCAG AAGACAGGAC TGGTGCCGCC CACTGTCCTG
    CAGTCCACAG CAGAGGAACT AGACCTTTAC AGTAACTCCG ATATTTTCAG TGACCTCTGC
    ACCGCCGCAC TCACCGATAC GGAGGACGAT ATCGGTGGGA CAAATCCTGC TCAGGACGGC
    AGATTTACAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335410.1
    CDS274..4128
    Translation

    Target ORF information:

    RefSeq Version XM_021479735.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GCTGACACTT CATCCAGCCT CGGCTCAGAG CGAGCGGCTC TTCCTAGAAA GCGTTTCACC
    TTACAGGGCC TGTCAAACCG CAGATCTCTG CCCGCAGAAC CCGCACCTAA GGAGACAGAA
    GTCACTCGCC GCTTTTCTTT AGCCTCAACT GTCGGCTCTG CCACTGCTCC ACGCACTAAA
    GCTCCTCTCT CCTCTAGCCT TAAGAGTAAG AGGCATCAGA TCAAGAGCGA CCCTACACCA
    TTTGGCTATG AAGGTGCTAC CAAGACACGC GGTTTTAAAA AAGAAACCAT GCGAGATTCC
    CTTCCAGCCG AGAGAAAAAG TAAAGGTAGC GGCGGTTCGA AGCCCGCTGT GCCGAGGTCT
    GCGTCTCAGC CAGGATGGAG TCAACGGCGT CTGCCTTCAA TGGACGCTGA GGATTTCGAG
    ACCATTCCGT CTAGTGCTGA AGAGCTGTCT ATTTCCTCTG ATGGAGAGGA TGAAGGACAC
    AACAAAAACG GGGATAATGA CCGCTATCAG GTGCAGTTGA CTTACGAGTC TTGCACTGCT
    CAGGATCTTT CTGTAGAAGC TGGAGATTTT GTACAATTCC TGGAAGAGTC AGAGAACGGC
    CTCTGGCTAG TCAAGAACCT TAGAACTCAG AAGACAGGAC TGGTGCCGCC CACTGTCCTG
    CAGTCCACAG CAGAGGAACT AGACCTTTAC AGTAACTCCG ATATTTTCAG TGACCTCTGC
    ACCGCCGCAC TCACCGATAC GGAGGACGAT ATCGGTGGGA CAAATCCTGC TCAGGACGGC
    AGATTTACAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147962
    Clone ID Related Accession (Same CDS sequence) XM_021479736.1
    Accession Version XM_021479736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3852bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGCC AGTCAGCTCC ATCAGAAGAT TACAGAGAAC
    ATCTACCATG CCCCCATGAG AAATATGCGT AAAACGGCCC TCAGACAGTC CGACTCTTCC
    AGTCCTGAAA CAGGCTACAG ACGCAGTGAC TCGGGTCCAA ATATGCGTCA GAAACGAGCT
    GACACTTCAT CCAGCCTCGG CTCAGAGCGA GCGGCTCTTC CTAGAAAGCG TTTCACCTTA
    CAGGGCCTGT CAAACCGCAG ATCTCTGCCC GCAGAACCCG CACCTAAGGA GACAGAAGTC
    ACTCGCCGCT TTTCTTTAGC CTCAACTGTC GGCTCTGCCA CTGCTCCACG CACTAAAGCT
    CCTCTCTCCT CTAGCCTTAA GAGTAAGAGG CATCAGATCA AGAGCGACCC TACACCATTT
    GGCTATGAAG GTGCTACCAA GACACGCGGT TTTAAAAAAG AAACCATGCG AGATTCCCTT
    CCAGCCGAGA GAAAAAGTAA AGGTAGCGGC GGTTCGAAGC CCGCTGTGCC GAGGTCTGCG
    TCTCAGCCAG GATGGAGTCA ACGGCGTCTG CCTTCAATGG ACGCTGAGGA TTTCGAGACC
    ATTCCGTCTA GTGCTGAAGA GCTGTCTATT TCCTCTGATG GAGAGGATGA AGGACACAAC
    AAAAACGGGG ATAATGACCG CTATCAGGTG CAGTTGACTT ACGAGTCTTG CACTGCTCAG
    GATCTTTCTG TAGAAGCTGG AGATTTTGTA CAATTCCTGG AAGAGTCAGA GAACGGCCTC
    TGGCTAGTCA AGAACCTTAG AACTCAGAAG ACAGGACTGG TGCCGCCCAC TGTCCTGCAG
    TCCACAGCAG AGGAACTAGA CCTTTACAGT AACTCCGATA TTTTCAGTGA CCTCTGCACC
    GCCGCACTCA CCGATACGGA GGACGATATC GGTGGGACAA ATCCTGCTCA GGACGGCAGA
    TTTACATGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335411.1
    CDS275..4126
    Translation

    Target ORF information:

    RefSeq Version XM_021479736.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGCC AGTCAGCTCC ATCAGAAGAT TACAGAGAAC
    ATCTACCATG CCCCCATGAG AAATATGCGT AAAACGGCCC TCAGACAGTC CGACTCTTCC
    AGTCCTGAAA CAGGCTACAG ACGCAGTGAC TCGGGTCCAA ATATGCGTCA GAAACGAGCT
    GACACTTCAT CCAGCCTCGG CTCAGAGCGA GCGGCTCTTC CTAGAAAGCG TTTCACCTTA
    CAGGGCCTGT CAAACCGCAG ATCTCTGCCC GCAGAACCCG CACCTAAGGA GACAGAAGTC
    ACTCGCCGCT TTTCTTTAGC CTCAACTGTC GGCTCTGCCA CTGCTCCACG CACTAAAGCT
    CCTCTCTCCT CTAGCCTTAA GAGTAAGAGG CATCAGATCA AGAGCGACCC TACACCATTT
    GGCTATGAAG GTGCTACCAA GACACGCGGT TTTAAAAAAG AAACCATGCG AGATTCCCTT
    CCAGCCGAGA GAAAAAGTAA AGGTAGCGGC GGTTCGAAGC CCGCTGTGCC GAGGTCTGCG
    TCTCAGCCAG GATGGAGTCA ACGGCGTCTG CCTTCAATGG ACGCTGAGGA TTTCGAGACC
    ATTCCGTCTA GTGCTGAAGA GCTGTCTATT TCCTCTGATG GAGAGGATGA AGGACACAAC
    AAAAACGGGG ATAATGACCG CTATCAGGTG CAGTTGACTT ACGAGTCTTG CACTGCTCAG
    GATCTTTCTG TAGAAGCTGG AGATTTTGTA CAATTCCTGG AAGAGTCAGA GAACGGCCTC
    TGGCTAGTCA AGAACCTTAG AACTCAGAAG ACAGGACTGG TGCCGCCCAC TGTCCTGCAG
    TCCACAGCAG AGGAACTAGA CCTTTACAGT AACTCCGATA TTTTCAGTGA CCTCTGCACC
    GCCGCACTCA CCGATACGGA GGACGATATC GGTGGGACAA ATCCTGCTCA GGACGGCAGA
    TTTACATGCT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147963
    Clone ID Related Accession (Same CDS sequence) XM_021479737.1
    Accession Version XM_021479737.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GATTGTTATG CTAAATTCCC TGTCTGACTT GCATGGCTAC
    ATTGATAAAG GCCAGTTGAC CTGTGACCTT GGGGGAACCC TGGATTATTG CCACAGTCAA
    TGGATTCATC ATCGCACAGC CATTGAGAAC TTTGCGCTGA CAGTGAAGAG CACAGCACAG
    ATGCTGCAGC AGTTCGGAAC AGACCTGGCA GAGACTGAAC TTCCCAATGA CGTGCAGTGC
    ACCAAAGATT TGCTCATCAC ACACACTGAA AAACACGATA AGCTCAAGGA TGAACTAAAA
    TTGGCTGTAA AACAAGGGAA TACTCTGCTG TCCTGTATTA AAGACCAGGC CACCAAAGCC
    GAGAGCCACA TTTTGAACCC AGACGAGGTT GAGAACCAAA CAACCGTTGA GAGGTTGTTA
    GCGCAGCTTG ATGAGACGGA GAATGCATTT GAACAATTCT GGTCAAGACA TCACCTCAAG
    TTAGAGCAGT GCCTACAGTT ACGACACTTT GAGCAGGACT TCAAAGAGGT GAAAGTGTCA
    CTGGACGGCC TCTCTGAAGC TTTGCTGGGA CTGTCAGATA CCGGGGACTG CGTGGCTCGT
    GTGGAACAGC TATTAGGAGA ACTGAAAACC TTGGAGGATA AAGCTCAGGA GTGTTTGGAG
    AAAGCTCAGC TTCAAGCTCA GCATGGGGAT CAGCTCATTC AGAGTAATCA CTACGCCATC
    GACTCCATCC GCCCCAAGTG TGTGGAACTG CGTCGGATCT GTGACGACTT CAGCAACGAG
    GCCAAAAAGA AGCGTGACGT TCTGACCAAA TCACTTGAAA TCCTCAAACG AATTGACGAG
    GTGAACCAGT GGTGTGAAAG CGGAGTGTAT CTGCTGGCCT CTCAGGCTGT AGACAGATGT
    CAAACACAGG AAGGTGCAGA AGCAGCACTG CAGGACATCG AGAGGTACAT GAAAACAGCC
    AAAGAGCAGC AGCTCAGTCA CCTCAAAGAA CTCAACAACC AGTATGAGGT CGTCCTCTCA
    GAGCTGCTCA AGAGCAAAGT TCAAACTGCC TTGAAAACGC TGAACGATGT CCAAGAGATG
    TTTGAGAAAA GACATGCAAG TCTGAAAAAG CTTTCCACGA AGCAAACAAG GCCTGTGCAA
    CCTGTTGCAC CCCGACCAGA ATCTTCACCA AAACGCCCCT CACCTAAAAT CCCACATCCC
    ACTCCTCCAA CATCACACCG CAAAACTACA GATAACTCCA GCACCAATAG ACAGCCCAGT
    GATGCAGAAC TGGCAAAAAG AAAAAACATT CGCAAAGCCA AAGGTGGCAT TAAGATTGAG
    GTCATGCATG AAGGAGGTCA AGGAGGTTCA AGTCATATTT TAATGAACAA TGAAACTGAA
    GAGTCGCTTT CAAACAGACG CAGTCACATT ATGAATGAAC TAATTGAGAC GGAAAGGCTG
    TATGTGGAAG AGCTGCAGAG CATCATAGAG GGCTATGCCG CGGCACTCGA TGACCCAGAA
    CTCATCTATC TGATCCCACC ATCCCTGGAA AACAAGAAGG AAGTTCTCTT TGGAAATCTG
    CCAGAAATTT ACCAATTCCA CCAGAAGATA TTTCTCAAAG AGCTGGAAAA TTCTGCCGAA
    AAGCCGGAGT TGGTGGGAAC ATGTTTTTTG AAGCGGAAAG AAGAGTTGCA GATTTATGAG
    AAATATTGTC AGAATAAGCC ACGCTCTGAA GCTTTGTGGC GGCAATGCGG GGACAGTATG
    TTCTTTCAGG AGTGTCAGAA AAGGTTGGAC CACAAGCTTT CATTAGACGC GTATCTGCTA
    AAGCCTGTGC AGAGAATCAC CAAATACCAG CTTATGTTAA AGGAGATGCT GAAATGCAGT
    AAGAACCCAG AAGGCACCGC TGAGCTGGAG GAAGCTCAGG CCACAGTGCT GGACATCATC
    AAATCGGTCA ACGACTCCAT GCATCAGATC GCCATCACTG GTTTTGAGGG CAGTTTGAAT
    GATCTGGGGA AACTGCTTAT GCAGGGGTCG TTTAACGTCT GGACAGACCA TAAGAAAGGC
    CATAGTAAGG TGAAGGACTT GGCACGCTTC AAACCCATGC AGAGACATCT CTTCCTCTAC
    CACAAAATGA TATTGTTCTG CAAGAAACGT GAGGAGATGA CTGATGGCCA TGAAAAATCC
    CCTTCCTACA GTTTCAAGCA CTCATTAAAG ATGGGCTCAG TGGGAATCAC AGAAAATGTA
    AAGGGAGACA TCAAGAAGTT TGAAATCTGG TACAATGGCA GAGAAGAAGT TTACATCATT
    CAGGCCCCGT CCATGGACGT GAAGAACACG TGGGTATCAG AAATCCGCAA AGTCTTAACA
    AGCCAGCTGG AAGCCTGCCG AGAAGCCAGT CAGCTCCATC AGAAGATTAC AGAGAACATC
    TACCATGCCC CCATGAGAAA TATGCGTAAA ACGGCCCTCA GACAGTCCGA CTCTTCCAGT
    CCTGAAACAG GCTACAGACG CAGTGACTCG GGTCCAAATA TGCGTCAGAA ACGAGCTGAC
    ACTTCATCCA GCCTCGGCTC AGAGCGAGCG GCTCTTCCTA GAAAGCGTTT CACCTTACAG
    GGCCTGTCAA ACCGCAGATC TCTGCCCGCA GAACCCGCAC CTAAGGAGAC AGAAGTCACT
    CGCCGCTTTT CTTTAGCCTC AACTGTCGGC TCTGCCACTG CTCCACGCAC TAAAGCTCCT
    CTCTCCTCTA GCCTTAAGAG TAAGAGGCAT CAGATCAAGA GCGACCCTAC ACCATTTGGC
    TATGAAGGTG CTACCAAGAC ACGCGGTTTT AAAAAAGAAA CCATGCGAGA TTCCCTTCCA
    GCCGAGAGAA AAAGTAAAGG TAGCGGCGGT TCGAAGCCCG CTGTGCCGAG GTCTGCGTCT
    CAGCCAGGAT GGAGTCAACG GCGTCTGCCT TCAATGGACG CTGAGGATTT CGAGACCATT
    CCGTCTAGTG CTGAAGAGCT GTCTATTTCC TCTGATGGAG AGGATGAAGG ACACAACAAA
    AACGGGGATA ATGACCGCTA TCAGGTGCAG TTGACTTACG AGTCTTGCAC TGCTCAGGAT
    CTTTCTGTAG AAGCTGGAGA TTTTGTACAA TTCCTGGAAG AGTCAGAGAA CGGCCTCTGG
    CTAGTCAAGA ACCTTAGAAC TCAGAAGACA GGACTGGTGC CGCCCACTGT CCTGCAGTCC
    ACAGCAGAGG AACTAGACCT TTACAGTAAC TCCGATATTT TCAGTGACCT CTGCACCGCC
    GCACTCACCG ATACGGAGGA CGATATCGGT GGGACAAATC CTGCTCAGGA CGGCAGATTT
    ACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335412.1
    CDS275..4123
    Translation

    Target ORF information:

    RefSeq Version XM_021479737.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479737.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GATTGTTATG CTAAATTCCC TGTCTGACTT GCATGGCTAC
    ATTGATAAAG GCCAGTTGAC CTGTGACCTT GGGGGAACCC TGGATTATTG CCACAGTCAA
    TGGATTCATC ATCGCACAGC CATTGAGAAC TTTGCGCTGA CAGTGAAGAG CACAGCACAG
    ATGCTGCAGC AGTTCGGAAC AGACCTGGCA GAGACTGAAC TTCCCAATGA CGTGCAGTGC
    ACCAAAGATT TGCTCATCAC ACACACTGAA AAACACGATA AGCTCAAGGA TGAACTAAAA
    TTGGCTGTAA AACAAGGGAA TACTCTGCTG TCCTGTATTA AAGACCAGGC CACCAAAGCC
    GAGAGCCACA TTTTGAACCC AGACGAGGTT GAGAACCAAA CAACCGTTGA GAGGTTGTTA
    GCGCAGCTTG ATGAGACGGA GAATGCATTT GAACAATTCT GGTCAAGACA TCACCTCAAG
    TTAGAGCAGT GCCTACAGTT ACGACACTTT GAGCAGGACT TCAAAGAGGT GAAAGTGTCA
    CTGGACGGCC TCTCTGAAGC TTTGCTGGGA CTGTCAGATA CCGGGGACTG CGTGGCTCGT
    GTGGAACAGC TATTAGGAGA ACTGAAAACC TTGGAGGATA AAGCTCAGGA GTGTTTGGAG
    AAAGCTCAGC TTCAAGCTCA GCATGGGGAT CAGCTCATTC AGAGTAATCA CTACGCCATC
    GACTCCATCC GCCCCAAGTG TGTGGAACTG CGTCGGATCT GTGACGACTT CAGCAACGAG
    GCCAAAAAGA AGCGTGACGT TCTGACCAAA TCACTTGAAA TCCTCAAACG AATTGACGAG
    GTGAACCAGT GGTGTGAAAG CGGAGTGTAT CTGCTGGCCT CTCAGGCTGT AGACAGATGT
    CAAACACAGG AAGGTGCAGA AGCAGCACTG CAGGACATCG AGAGGTACAT GAAAACAGCC
    AAAGAGCAGC AGCTCAGTCA CCTCAAAGAA CTCAACAACC AGTATGAGGT CGTCCTCTCA
    GAGCTGCTCA AGAGCAAAGT TCAAACTGCC TTGAAAACGC TGAACGATGT CCAAGAGATG
    TTTGAGAAAA GACATGCAAG TCTGAAAAAG CTTTCCACGA AGCAAACAAG GCCTGTGCAA
    CCTGTTGCAC CCCGACCAGA ATCTTCACCA AAACGCCCCT CACCTAAAAT CCCACATCCC
    ACTCCTCCAA CATCACACCG CAAAACTACA GATAACTCCA GCACCAATAG ACAGCCCAGT
    GATGCAGAAC TGGCAAAAAG AAAAAACATT CGCAAAGCCA AAGGTGGCAT TAAGATTGAG
    GTCATGCATG AAGGAGGTCA AGGAGGTTCA AGTCATATTT TAATGAACAA TGAAACTGAA
    GAGTCGCTTT CAAACAGACG CAGTCACATT ATGAATGAAC TAATTGAGAC GGAAAGGCTG
    TATGTGGAAG AGCTGCAGAG CATCATAGAG GGCTATGCCG CGGCACTCGA TGACCCAGAA
    CTCATCTATC TGATCCCACC ATCCCTGGAA AACAAGAAGG AAGTTCTCTT TGGAAATCTG
    CCAGAAATTT ACCAATTCCA CCAGAAGATA TTTCTCAAAG AGCTGGAAAA TTCTGCCGAA
    AAGCCGGAGT TGGTGGGAAC ATGTTTTTTG AAGCGGAAAG AAGAGTTGCA GATTTATGAG
    AAATATTGTC AGAATAAGCC ACGCTCTGAA GCTTTGTGGC GGCAATGCGG GGACAGTATG
    TTCTTTCAGG AGTGTCAGAA AAGGTTGGAC CACAAGCTTT CATTAGACGC GTATCTGCTA
    AAGCCTGTGC AGAGAATCAC CAAATACCAG CTTATGTTAA AGGAGATGCT GAAATGCAGT
    AAGAACCCAG AAGGCACCGC TGAGCTGGAG GAAGCTCAGG CCACAGTGCT GGACATCATC
    AAATCGGTCA ACGACTCCAT GCATCAGATC GCCATCACTG GTTTTGAGGG CAGTTTGAAT
    GATCTGGGGA AACTGCTTAT GCAGGGGTCG TTTAACGTCT GGACAGACCA TAAGAAAGGC
    CATAGTAAGG TGAAGGACTT GGCACGCTTC AAACCCATGC AGAGACATCT CTTCCTCTAC
    CACAAAATGA TATTGTTCTG CAAGAAACGT GAGGAGATGA CTGATGGCCA TGAAAAATCC
    CCTTCCTACA GTTTCAAGCA CTCATTAAAG ATGGGCTCAG TGGGAATCAC AGAAAATGTA
    AAGGGAGACA TCAAGAAGTT TGAAATCTGG TACAATGGCA GAGAAGAAGT TTACATCATT
    CAGGCCCCGT CCATGGACGT GAAGAACACG TGGGTATCAG AAATCCGCAA AGTCTTAACA
    AGCCAGCTGG AAGCCTGCCG AGAAGCCAGT CAGCTCCATC AGAAGATTAC AGAGAACATC
    TACCATGCCC CCATGAGAAA TATGCGTAAA ACGGCCCTCA GACAGTCCGA CTCTTCCAGT
    CCTGAAACAG GCTACAGACG CAGTGACTCG GGTCCAAATA TGCGTCAGAA ACGAGCTGAC
    ACTTCATCCA GCCTCGGCTC AGAGCGAGCG GCTCTTCCTA GAAAGCGTTT CACCTTACAG
    GGCCTGTCAA ACCGCAGATC TCTGCCCGCA GAACCCGCAC CTAAGGAGAC AGAAGTCACT
    CGCCGCTTTT CTTTAGCCTC AACTGTCGGC TCTGCCACTG CTCCACGCAC TAAAGCTCCT
    CTCTCCTCTA GCCTTAAGAG TAAGAGGCAT CAGATCAAGA GCGACCCTAC ACCATTTGGC
    TATGAAGGTG CTACCAAGAC ACGCGGTTTT AAAAAAGAAA CCATGCGAGA TTCCCTTCCA
    GCCGAGAGAA AAAGTAAAGG TAGCGGCGGT TCGAAGCCCG CTGTGCCGAG GTCTGCGTCT
    CAGCCAGGAT GGAGTCAACG GCGTCTGCCT TCAATGGACG CTGAGGATTT CGAGACCATT
    CCGTCTAGTG CTGAAGAGCT GTCTATTTCC TCTGATGGAG AGGATGAAGG ACACAACAAA
    AACGGGGATA ATGACCGCTA TCAGGTGCAG TTGACTTACG AGTCTTGCAC TGCTCAGGAT
    CTTTCTGTAG AAGCTGGAGA TTTTGTACAA TTCCTGGAAG AGTCAGAGAA CGGCCTCTGG
    CTAGTCAAGA ACCTTAGAAC TCAGAAGACA GGACTGGTGC CGCCCACTGT CCTGCAGTCC
    ACAGCAGAGG AACTAGACCT TTACAGTAAC TCCGATATTT TCAGTGACCT CTGCACCGCC
    GCACTCACCG ATACGGAGGA CGATATCGGT GGGACAAATC CTGCTCAGGA CGGCAGATTT
    ACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147964
    Clone ID Related Accession (Same CDS sequence) XM_021479738.1
    Accession Version XM_021479738.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GCTGACACTT CATCCAGCCT CGGCTCAGAG CGAGCGGCTC TTCCTAGAAA GCGTTTCACC
    TTACAGGGCC TGTCAAACCG CAGATCTCTG CCCGCAGAAC CCGCACCTAA GGAGACAGAA
    GTCACTCGCC GCTTTTCTTT AGCCTCAACT GTCGGCTCTG CCACTGCTCC ACGCACTAAA
    GCTCCTCTCT CCTCTAGCCT TAAGAGTAAG AGGCATCAGA TCAAGAGCGA CCCTACACCA
    TTTGGCTATG AAGAAACCAT GCGAGATTCC CTTCCAGCCG AGAGAAAAAG TAAAGGTAGC
    GGCGGTTCGA AGCCCGCTGT GCCGAGGTCT GCGTCTCAGC CAGGATGGAG TCAACGGCGT
    CTGCCTTCAA TGGACGCTGA GGATTTCGAG ACCATTCCGT CTAGTGCTGA AGAGCTGTCT
    ATTTCCTCTG ATGGAGAGGA TGAAGGACAC AACAAAAACG GGGATAATGA CCGCTATCAG
    GTGCAGTTGA CTTACGAGTC TTGCACTGCT CAGGATCTTT CTGTAGAAGC TGGAGATTTT
    GTACAATTCC TGGAAGAGTC AGAGAACGGC CTCTGGCTAG TCAAGAACCT TAGAACTCAG
    AAGACAGGAC TGGTGCCGCC CACTGTCCTG CAGTCCACAG CAGAGGAACT AGACCTTTAC
    AGTAACTCCG ATATTTTCAG TGACCTCTGC ACCGCCGCAC TCACCGATAC GGAGGACGAT
    ATCGGTGGGA CAAATCCTGC TCAGGACGGC AGATTTACAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335413.1
    CDS274..4098
    Translation

    Target ORF information:

    RefSeq Version XM_021479738.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479738.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GCTGACACTT CATCCAGCCT CGGCTCAGAG CGAGCGGCTC TTCCTAGAAA GCGTTTCACC
    TTACAGGGCC TGTCAAACCG CAGATCTCTG CCCGCAGAAC CCGCACCTAA GGAGACAGAA
    GTCACTCGCC GCTTTTCTTT AGCCTCAACT GTCGGCTCTG CCACTGCTCC ACGCACTAAA
    GCTCCTCTCT CCTCTAGCCT TAAGAGTAAG AGGCATCAGA TCAAGAGCGA CCCTACACCA
    TTTGGCTATG AAGAAACCAT GCGAGATTCC CTTCCAGCCG AGAGAAAAAG TAAAGGTAGC
    GGCGGTTCGA AGCCCGCTGT GCCGAGGTCT GCGTCTCAGC CAGGATGGAG TCAACGGCGT
    CTGCCTTCAA TGGACGCTGA GGATTTCGAG ACCATTCCGT CTAGTGCTGA AGAGCTGTCT
    ATTTCCTCTG ATGGAGAGGA TGAAGGACAC AACAAAAACG GGGATAATGA CCGCTATCAG
    GTGCAGTTGA CTTACGAGTC TTGCACTGCT CAGGATCTTT CTGTAGAAGC TGGAGATTTT
    GTACAATTCC TGGAAGAGTC AGAGAACGGC CTCTGGCTAG TCAAGAACCT TAGAACTCAG
    AAGACAGGAC TGGTGCCGCC CACTGTCCTG CAGTCCACAG CAGAGGAACT AGACCTTTAC
    AGTAACTCCG ATATTTTCAG TGACCTCTGC ACCGCCGCAC TCACCGATAC GGAGGACGAT
    ATCGGTGGGA CAAATCCTGC TCAGGACGGC AGATTTACAT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147965
    Clone ID Related Accession (Same CDS sequence) XM_021479739.1
    Accession Version XM_021479739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GCTGACACTT CATCCAGCCT CGGCTCAGAG CGAGCGGCTC TTCCTAGAAA GCGTTTCACC
    TTACAGGGCC TGTCAAACCG CAGATCTCTG CCCGCAGAAC CCGCACCTAA GGAGACAGAA
    GTCACTCGCC GCTTTTCTTT AGCCTCAACT GTCGGCTCTG CCACTGCTCC ACGCACTAAA
    GCTCCTCTCT CCTCTAGCCT TAAGAGTAAG AGGCATCAGA TCAAGAGCGA CCCTACACCA
    TTTGGCTATG AAGGTGCTAC CAAGACACGC GGTTTTAAAA AAGAAACCAT GCGAGATTCC
    CTTCCAGCCG AGAGAAAAAG TAAAGGTAGC GGCGGTTCGA AGCCCGCTGT GCCGAGGTCT
    GCGTCTCAGC CAGGATGGAG TCAACGGCGT CTGCCTTCAA TGGACGCTGA GGATTTCGAG
    ACCATTCCGT CTAGTGCTGA AGAGCTGTCT ATTTCCTCTG ATGGAGAGGA TGAAGGACAC
    AACAAAAACG GGGATAATGA CCGCTATCAG GTGCAGTTGA CTTACGAGTC TTGCACTGCT
    CAGGATCTTT CTGTAGAAGC TGGAGATTTT GTACAATTCC TGGAAGAGTC AGAGAACGGC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335414.1
    CDS273..3938
    Translation

    Target ORF information:

    RefSeq Version XM_021479739.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GCTGACACTT CATCCAGCCT CGGCTCAGAG CGAGCGGCTC TTCCTAGAAA GCGTTTCACC
    TTACAGGGCC TGTCAAACCG CAGATCTCTG CCCGCAGAAC CCGCACCTAA GGAGACAGAA
    GTCACTCGCC GCTTTTCTTT AGCCTCAACT GTCGGCTCTG CCACTGCTCC ACGCACTAAA
    GCTCCTCTCT CCTCTAGCCT TAAGAGTAAG AGGCATCAGA TCAAGAGCGA CCCTACACCA
    TTTGGCTATG AAGGTGCTAC CAAGACACGC GGTTTTAAAA AAGAAACCAT GCGAGATTCC
    CTTCCAGCCG AGAGAAAAAG TAAAGGTAGC GGCGGTTCGA AGCCCGCTGT GCCGAGGTCT
    GCGTCTCAGC CAGGATGGAG TCAACGGCGT CTGCCTTCAA TGGACGCTGA GGATTTCGAG
    ACCATTCCGT CTAGTGCTGA AGAGCTGTCT ATTTCCTCTG ATGGAGAGGA TGAAGGACAC
    AACAAAAACG GGGATAATGA CCGCTATCAG GTGCAGTTGA CTTACGAGTC TTGCACTGCT
    CAGGATCTTT CTGTAGAAGC TGGAGATTTT GTACAATTCC TGGAAGAGTC AGAGAACGGC
    CTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147966
    Clone ID Related Accession (Same CDS sequence) XM_021479740.1
    Accession Version XM_021479740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3483bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GGATGGAGTC AACGGCGTCT GCCTTCAATG GACGCTGAGG ATTTCGAGAC CATTCCGTCT
    AGTGCTGAAG AGCTGTCTAT TTCCTCTGAT GGAGAGGATG AAGGACACAA CAAAAACGGG
    GATAATGACC GCTATCAGGT GCAGTTGACT TACGAGTCTT GCACTGCTCA GGATCTTTCT
    GTAGAAGCTG GAGATTTTGT ACAATTCCTG GAAGAGTCAG AGAACGGCCT CTGGCTAGTC
    AAGAACCTTA GAACTCAGAA GACAGGACTG GTGCCGCCCA CTGTCCTGCA GTCCACAGCA
    GAGGAACTAG ACCTTTACAG TAACTCCGAT ATTTTCAGTG ACCTCTGCAC CGCCGCACTC
    ACCGATACGG AGGACGATAT CGGTGGGACA AATCCTGCTC AGGACGGCAG ATTTACATGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021335415.1
    CDS271..3753
    Translation

    Target ORF information:

    RefSeq Version XM_021479740.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021479740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAACGGCT ACCAAACTGC CCAGTCCCGT TGCTCGGTCT GTGGACTGTA CTCACAGGGA 
    GCGTCCTTTA CTTTGGTGCG CATCTCAGCT GGAGAAATGG AATCCTATCA TCGTTTCCTG
    GGAGTGTGTC TGGAGACTGA ATTAGCAACG AGGAGAAATG AGATTATGCA GCAGGAGATC
    AGGCCGTTGC TGGCTGTCGA CATCATTGAA CAACTTCACA GGCAGTTTGC TCTTCTTTCA
    GGAGGAAGAG GACAAGAGGG TTCTCCCATC ATCACCTTCC CAGAGTTCTC AGGTTTTAAT
    GAGGTTTCAG ATGAGGATTT TATTAATGTT GTCACTTACC TGACCAGCAT ACCTAGCATG
    GATGCTGCTA GTATCGGCTT CATCATCATC TTGGACCGAA GGAAGGATAA ATGGAACTCA
    GCAATCGCTT CTCTGGCACG CATAGCGGGC TCCTTTCCAG GAAACCTACA GCTAGTTCTA
    GTTCTGAAAC CTTCGCGCTT CCTCCAGAGG GCCATCGCTG ACGTCAGTAT GAGGTTACAC
    AGAGATGACT TCAAATTGAA GGTACCGATT GTTATGCTAA ATTCCCTGTC TGACTTGCAT
    GGCTACATTG ATAAAGGCCA GTTGACCTGT GACCTTGGGG GAACCCTGGA TTATTGCCAC
    AGTCAATGGA TTCATCATCG CACAGCCATT GAGAACTTTG CGCTGACAGT GAAGAGCACA
    GCACAGATGC TGCAGCAGTT CGGAACAGAC CTGGCAGAGA CTGAACTTCC CAATGACGTG
    CAGTGCACCA AAGATTTGCT CATCACACAC ACTGAAAAAC ACGATAAGCT CAAGGATGAA
    CTAAAATTGG CTGTAAAACA AGGGAATACT CTGCTGTCCT GTATTAAAGA CCAGGCCACC
    AAAGCCGAGA GCCACATTTT GAACCCAGAC GAGGTTGAGA ACCAAACAAC CGTTGAGAGG
    TTGTTAGCGC AGCTTGATGA GACGGAGAAT GCATTTGAAC AATTCTGGTC AAGACATCAC
    CTCAAGTTAG AGCAGTGCCT ACAGTTACGA CACTTTGAGC AGGACTTCAA AGAGGTGAAA
    GTGTCACTGG ACGGCCTCTC TGAAGCTTTG CTGGGACTGT CAGATACCGG GGACTGCGTG
    GCTCGTGTGG AACAGCTATT AGGAGAACTG AAAACCTTGG AGGATAAAGC TCAGGAGTGT
    TTGGAGAAAG CTCAGCTTCA AGCTCAGCAT GGGGATCAGC TCATTCAGAG TAATCACTAC
    GCCATCGACT CCATCCGCCC CAAGTGTGTG GAACTGCGTC GGATCTGTGA CGACTTCAGC
    AACGAGGCCA AAAAGAAGCG TGACGTTCTG ACCAAATCAC TTGAAATCCT CAAACGAATT
    GACGAGGTGA ACCAGTGGTG TGAAAGCGGA GTGTATCTGC TGGCCTCTCA GGCTGTAGAC
    AGATGTCAAA CACAGGAAGG TGCAGAAGCA GCACTGCAGG ACATCGAGAG GTACATGAAA
    ACAGCCAAAG AGCAGCAGCT CAGTCACCTC AAAGAACTCA ACAACCAGTA TGAGGTCGTC
    CTCTCAGAGC TGCTCAAGAG CAAAGTTCAA ACTGCCTTGA AAACGCTGAA CGATGTCCAA
    GAGATGTTTG AGAAAAGACA TGCAAGTCTG AAAAAGCTTT CCACGAAGCA AACAAGGCCT
    GTGCAACCTG TTGCACCCCG ACCAGAATCT TCACCAAAAC GCCCCTCACC TAAAATCCCA
    CATCCCACTC CTCCAACATC ACACCGCAAA ACTACAGATA ACTCCAGCAC CAATAGACAG
    CCCAGTGATG CAGAACTGGC AAAAAGAAAA AACATTCGCA AAGCCAAAGG TGGCATTAAG
    ATTGAGGTCA TGCATGAAGG AGGTCAAGGA GGTTCAAGTC ATATTTTAAT GAACAATGAA
    ACTGAAGAGT CGCTTTCAAA CAGACGCAGT CACATTATGA ATGAACTAAT TGAGACGGAA
    AGGCTGTATG TGGAAGAGCT GCAGAGCATC ATAGAGGGCT ATGCCGCGGC ACTCGATGAC
    CCAGAACTCA TCTATCTGAT CCCACCATCC CTGGAAAACA AGAAGGAAGT TCTCTTTGGA
    AATCTGCCAG AAATTTACCA ATTCCACCAG AAGATATTTC TCAAAGAGCT GGAAAATTCT
    GCCGAAAAGC CGGAGTTGGT GGGAACATGT TTTTTGAAGC GGAAAGAAGA GTTGCAGATT
    TATGAGAAAT ATTGTCAGAA TAAGCCACGC TCTGAAGCTT TGTGGCGGCA ATGCGGGGAC
    AGTATGTTCT TTCAGGAGTG TCAGAAAAGG TTGGACCACA AGCTTTCATT AGACGCGTAT
    CTGCTAAAGC CTGTGCAGAG AATCACCAAA TACCAGCTTA TGTTAAAGGA GATGCTGAAA
    TGCAGTAAGA ACCCAGAAGG CACCGCTGAG CTGGAGGAAG CTCAGGCCAC AGTGCTGGAC
    ATCATCAAAT CGGTCAACGA CTCCATGCAT CAGATCGCCA TCACTGGTTT TGAGGGCAGT
    TTGAATGATC TGGGGAAACT GCTTATGCAG GGGTCGTTTA ACGTCTGGAC AGACCATAAG
    AAAGGCCATA GTAAGGTGAA GGACTTGGCA CGCTTCAAAC CCATGCAGAG ACATCTCTTC
    CTCTACCACA AAATGATATT GTTCTGCAAG AAACGTGAGG AGATGACTGA TGGCCATGAA
    AAATCCCCTT CCTACAGTTT CAAGCACTCA TTAAAGATGG GCTCAGTGGG AATCACAGAA
    AATGTAAAGG GAGACATCAA GAAGTTTGAA ATCTGGTACA ATGGCAGAGA AGAAGTTTAC
    ATCATTCAGG CCCCGTCCAT GGACGTGAAG AACACGTGGG TATCAGAAAT CCGCAAAGTC
    TTAACAAGCC AGCTGGAAGC CTGCCGAGAA GCCAGTCAGC TCCATCAGAA GATTACAGAG
    AACATCTACC ATGCCCCCAT GAGAAATATG CGTAAAACGG CCCTCAGACA GTCCGACTCT
    TCCAGTCCTG AAACAGGCTA CAGACGCAGT GACTCGGGTC CAAATATGCG TCAGAAACGA
    GGATGGAGTC AACGGCGTCT GCCTTCAATG GACGCTGAGG ATTTCGAGAC CATTCCGTCT
    AGTGCTGAAG AGCTGTCTAT TTCCTCTGAT GGAGAGGATG AAGGACACAA CAAAAACGGG
    GATAATGACC GCTATCAGGT GCAGTTGACT TACGAGTCTT GCACTGCTCA GGATCTTTCT
    GTAGAAGCTG GAGATTTTGT ACAATTCCTG GAAGAGTCAG AGAACGGCCT CTGGCTAGTC
    AAGAACCTTA GAACTCAGAA GACAGGACTG GTGCCGCCCA CTGTCCTGCA GTCCACAGCA
    GAGGAACTAG ACCTTTACAG TAACTCCGAT ATTTTCAGTG ACCTCTGCAC CGCCGCACTC
    ACCGATACGG AGGACGATAT CGGTGGGACA AATCCTGCTC AGGACGGCAG ATTTACATGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54310
    Clone ID Related Accession (Same CDS sequence) XM_017358283.1
    Accession Version XM_017358283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGATTG TTATGCTAAA TTCCCTGTCT GACTTGCATG GCTACATTGA TAAAGGCCAG
    TTGACCTGTG ACCTTGGGGG AACCCTGGAT TATTGCCACA GTCAATGGAT TCATCATCGC
    ACAGCCATTG AGAACTTTGC GCTGACAGTG AAGAGCACAG CACAGATGCT GCAGCAGTTC
    GGAACAGACC TGGCAGAGAC TGAACTTCCC AATGACGTGC AGTGCACCAA AGATTTGCTC
    ATCACACACA CTGAAAAACA CGATAAGCTC AAGGATGAAC TAAAATTGGC TGTAAAACAA
    GGGAATACTC TGCTGTCCTG TATTAAAGAC CAGGCCACCA AAGCCGAGAG CCACATTTTG
    AACCCAGACG AGGTTGAGAA CCAAACAACC GTTGAGAGGT TGTTAGCGCA GCTTGATGAG
    ACGGAGAATG CATTTGAACA ATTCTGGTCA AGACATCACC TCAAGTTAGA GCAGTGCCTA
    CAGTTACGAC ACTTTGAGCA GGACTTCAAA GAGGTGAAAG TGTCACTGGA CGGCCTCTCT
    GAAGCTTTGC TGGGACTGTC AGATACCGGG GACTGCGTGG CTCGTGTGGA ACAGCTATTA
    GGAGAACTGA AAACCTTGGA GGATAAAGCT CAGGAGTGTT TGGAGAAAGC TCAGCTTCAA
    GCTCAGCATG GGGATCAGCT CATTCAGAGT AATCACTACG CCATCGACTC CATCCGCCCC
    AAGTGTGTGG AACTGCGTCG GATCTGTGAC GACTTCAGCA ACGAGGCCAA AAAGAAGCGT
    GACGTTCTGA CCAAATCACT TGAAATCCTC AAACGAATTG ACGAGGTGAA CCAGTGGTGT
    GAAAGCGGAG TGTATCTGCT GGCCTCTCAG GCTGTAGACA GATGTCAAAC ACAGGAAGGT
    GCAGAAGCAG CACTGCAGGA CATCGAGAGG TACATGAAAA CAGCCAAAGA GCAGCAGCTC
    AGTCACCTCA AAGAACTCAA CAACCAGTAT GAGGTCGTCC TCTCAGAGCT GCTCAAGAGC
    AAAGTTCAAA CTGCCTTGAA AACGCTGAAC GATGTCCAAG AGATGTTTGA GAAAAGACAT
    GCAAGTCTGA AAAAGCTTTC CACGAAGCAA ACAAGGCCTG TGCAACCTGT TGCACCCCGA
    CCAGAATCTT CACCAAAACG CCCCTCACCT AAAATCCCAC ATCCCACTCC TCCAACATCA
    CACCGCAAAA CTACAGATAA CTCCAGCACC AATAGACAGC CCAGTGATGC AGAACTGGCA
    AAAAGAAAAA ACATTCGCAA AGCCAAAGGT GGCATTAAGA TTGAGGTCAT GCATGAAGGA
    GGTCAAGGAG GTTCAAGTCA TATTTTAATG AACAATGAAA CTGAAGAGTC GCTTTCAAAC
    AGACGCAGTC ACATTATGAA TGAACTAATT GAGACGGAAA GGCTGTATGT GGAAGAGCTG
    CAGAGCATCA TAGAGGGCTA TGCCGCGGCA CTCGATGACC CAGAACTCAT CTATCTGATC
    CCACCATCCC TGGAAAACAA GAAGGAAGTT CTCTTTGGAA ATCTGCCAGA AATTTACCAA
    TTCCACCAGA AGATATTTCT CAAAGAGCTG GAAAATTCTG CCGAAAAGCC GGAGTTGGTG
    GGAACATGTT TTTTGAAGCG GAAAGAAGAG TTGCAGATTT ATGAGAAATA TTGTCAGAAT
    AAGCCACGCT CTGAAGCTTT GTGGCGGCAA TGCGGGGACA GTATGTTCTT TCAGGAGTGT
    CAGAAAAGGT TGGACCACAA GCTTTCATTA GACGCGTATC TGCTAAAGCC TGTGCAGAGA
    ATCACCAAAT ACCAGCTTAT GTTAAAGGAG ATGCTGAAAT GCAGTAAGAA CCCAGAAGGC
    ACCGCTGAGC TGGAGGAAGC TCAGGCCACA GTGCTGGACA TCATCAAATC GGTCAACGAC
    TCCATGCATC AGATCGCCAT CACTGGTTTT GAGGGCAGTT TGAATGATCT GGGGAAACTG
    CTTATGCAGG GGTCGTTTAA CGTCTGGACA GACCATAAGA AAGGCCATAG TAAGGTGAAG
    GACTTGGCAC GCTTCAAACC CATGCAGAGA CATCTCTTCC TCTACCACAA AATGATATTG
    TTCTGCAAGA AACGTGAGGA GATGACTGAT GGCCATGAAA AATCCCCTTC CTACAGTTTC
    AAGCACTCAT TAAAGATGGG CTCAGTGGGA ATCACAGAAA ATGTAAAGGG AGACATCAAG
    AAGTTTGAAA TCTGGTACAA TGGCAGAGAA GAAGTTTACA TCATTCAGGC CCCGTCCATG
    GACGTGAAGA ACACGTGGGT ATCAGAAATC CGCAAAGTCT TAACAAGCCA GCTGGAAGCC
    TGCCGAGAAG CCAGTCAGCT CCATCAGAAG ATTACAGAGA ACATCTACCA TGCCCCCATG
    AGAAATATGC GTAAAACGGC CCTCAGACAG TCCGACTCTT CCAGTCCTGA AACAGGCTAC
    AGACGCAGTG ACTCGGGTCC AAATATGCGT CAGAAACGAG CTGACACTTC ATCCAGCCTC
    GGCTCAGAGC GAGCGGCTCT TCCTAGAAAG CGTTTCACCT TACAGGGCCT GTCAAACCGC
    AGATCTCTGC CCGCAGAACC CGCACCTAAG GAGACAGAAG TCACTCGCCG CTTTTCTTTA
    GCCTCAACTG TCGGCTCTGC CACTGCTCCA CGCACTAAAG CTCCTCTCTC CTCTAGCCTT
    AAGAGTAAGA GGCATCAGAT CAAGAGCGAC CCTACACCAT TTGGCTATGA AGGTGCTACC
    AAGACACGCG GTTTTAAAAA AGAAACCATG CGAGATTCCC TTCCAGCCGA GAGAAAAAGT
    AAAGGTAGCG GCGGTTCGAA GCCCGCTGTG CCGAGGTCTG CGTCTCAGCC AGGATGGAGT
    CAACGGCGTC TGCCTTCAAT GGACGCTGAG GATTTCGAGA CCATTCCGTC TAGTGCTGAA
    GAGCTGTCTA TTTCCTCTGA TGGAGAGGAT GAAGGACACA ACAAAAACGG GGATAATGAC
    CGCTATCAGG TGCAGTTGAC TTACGAGTCT TGCACTGCTC AGGATCTTTC TGTAGAAGCT
    GGAGATTTTG TACAATTCCT GGAAGAGTCA GAGAACGGCC TCTGGCTAGT CAAGAACCTT
    AGAACTCAGA AGACAGGACT GGTGCCGCCC ACTGTCCTGC AGTCCACAGC AGAGGAACTA
    GACCTTTACA GTAACTCCGA TATTTTCAGT GACCTCTGCA CCGCCGCACT CACCGATACG
    GAGGACGATA TCGGTGGGAC AAATCCTGCT CAGGACGGCA GATTTACATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213772.1
    CDS330..4103
    Translation

    Target ORF information:

    RefSeq Version XM_017358283.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGATTG TTATGCTAAA TTCCCTGTCT GACTTGCATG GCTACATTGA TAAAGGCCAG
    TTGACCTGTG ACCTTGGGGG AACCCTGGAT TATTGCCACA GTCAATGGAT TCATCATCGC
    ACAGCCATTG AGAACTTTGC GCTGACAGTG AAGAGCACAG CACAGATGCT GCAGCAGTTC
    GGAACAGACC TGGCAGAGAC TGAACTTCCC AATGACGTGC AGTGCACCAA AGATTTGCTC
    ATCACACACA CTGAAAAACA CGATAAGCTC AAGGATGAAC TAAAATTGGC TGTAAAACAA
    GGGAATACTC TGCTGTCCTG TATTAAAGAC CAGGCCACCA AAGCCGAGAG CCACATTTTG
    AACCCAGACG AGGTTGAGAA CCAAACAACC GTTGAGAGGT TGTTAGCGCA GCTTGATGAG
    ACGGAGAATG CATTTGAACA ATTCTGGTCA AGACATCACC TCAAGTTAGA GCAGTGCCTA
    CAGTTACGAC ACTTTGAGCA GGACTTCAAA GAGGTGAAAG TGTCACTGGA CGGCCTCTCT
    GAAGCTTTGC TGGGACTGTC AGATACCGGG GACTGCGTGG CTCGTGTGGA ACAGCTATTA
    GGAGAACTGA AAACCTTGGA GGATAAAGCT CAGGAGTGTT TGGAGAAAGC TCAGCTTCAA
    GCTCAGCATG GGGATCAGCT CATTCAGAGT AATCACTACG CCATCGACTC CATCCGCCCC
    AAGTGTGTGG AACTGCGTCG GATCTGTGAC GACTTCAGCA ACGAGGCCAA AAAGAAGCGT
    GACGTTCTGA CCAAATCACT TGAAATCCTC AAACGAATTG ACGAGGTGAA CCAGTGGTGT
    GAAAGCGGAG TGTATCTGCT GGCCTCTCAG GCTGTAGACA GATGTCAAAC ACAGGAAGGT
    GCAGAAGCAG CACTGCAGGA CATCGAGAGG TACATGAAAA CAGCCAAAGA GCAGCAGCTC
    AGTCACCTCA AAGAACTCAA CAACCAGTAT GAGGTCGTCC TCTCAGAGCT GCTCAAGAGC
    AAAGTTCAAA CTGCCTTGAA AACGCTGAAC GATGTCCAAG AGATGTTTGA GAAAAGACAT
    GCAAGTCTGA AAAAGCTTTC CACGAAGCAA ACAAGGCCTG TGCAACCTGT TGCACCCCGA
    CCAGAATCTT CACCAAAACG CCCCTCACCT AAAATCCCAC ATCCCACTCC TCCAACATCA
    CACCGCAAAA CTACAGATAA CTCCAGCACC AATAGACAGC CCAGTGATGC AGAACTGGCA
    AAAAGAAAAA ACATTCGCAA AGCCAAAGGT GGCATTAAGA TTGAGGTCAT GCATGAAGGA
    GGTCAAGGAG GTTCAAGTCA TATTTTAATG AACAATGAAA CTGAAGAGTC GCTTTCAAAC
    AGACGCAGTC ACATTATGAA TGAACTAATT GAGACGGAAA GGCTGTATGT GGAAGAGCTG
    CAGAGCATCA TAGAGGGCTA TGCCGCGGCA CTCGATGACC CAGAACTCAT CTATCTGATC
    CCACCATCCC TGGAAAACAA GAAGGAAGTT CTCTTTGGAA ATCTGCCAGA AATTTACCAA
    TTCCACCAGA AGATATTTCT CAAAGAGCTG GAAAATTCTG CCGAAAAGCC GGAGTTGGTG
    GGAACATGTT TTTTGAAGCG GAAAGAAGAG TTGCAGATTT ATGAGAAATA TTGTCAGAAT
    AAGCCACGCT CTGAAGCTTT GTGGCGGCAA TGCGGGGACA GTATGTTCTT TCAGGAGTGT
    CAGAAAAGGT TGGACCACAA GCTTTCATTA GACGCGTATC TGCTAAAGCC TGTGCAGAGA
    ATCACCAAAT ACCAGCTTAT GTTAAAGGAG ATGCTGAAAT GCAGTAAGAA CCCAGAAGGC
    ACCGCTGAGC TGGAGGAAGC TCAGGCCACA GTGCTGGACA TCATCAAATC GGTCAACGAC
    TCCATGCATC AGATCGCCAT CACTGGTTTT GAGGGCAGTT TGAATGATCT GGGGAAACTG
    CTTATGCAGG GGTCGTTTAA CGTCTGGACA GACCATAAGA AAGGCCATAG TAAGGTGAAG
    GACTTGGCAC GCTTCAAACC CATGCAGAGA CATCTCTTCC TCTACCACAA AATGATATTG
    TTCTGCAAGA AACGTGAGGA GATGACTGAT GGCCATGAAA AATCCCCTTC CTACAGTTTC
    AAGCACTCAT TAAAGATGGG CTCAGTGGGA ATCACAGAAA ATGTAAAGGG AGACATCAAG
    AAGTTTGAAA TCTGGTACAA TGGCAGAGAA GAAGTTTACA TCATTCAGGC CCCGTCCATG
    GACGTGAAGA ACACGTGGGT ATCAGAAATC CGCAAAGTCT TAACAAGCCA GCTGGAAGCC
    TGCCGAGAAG CCAGTCAGCT CCATCAGAAG ATTACAGAGA ACATCTACCA TGCCCCCATG
    AGAAATATGC GTAAAACGGC CCTCAGACAG TCCGACTCTT CCAGTCCTGA AACAGGCTAC
    AGACGCAGTG ACTCGGGTCC AAATATGCGT CAGAAACGAG CTGACACTTC ATCCAGCCTC
    GGCTCAGAGC GAGCGGCTCT TCCTAGAAAG CGTTTCACCT TACAGGGCCT GTCAAACCGC
    AGATCTCTGC CCGCAGAACC CGCACCTAAG GAGACAGAAG TCACTCGCCG CTTTTCTTTA
    GCCTCAACTG TCGGCTCTGC CACTGCTCCA CGCACTAAAG CTCCTCTCTC CTCTAGCCTT
    AAGAGTAAGA GGCATCAGAT CAAGAGCGAC CCTACACCAT TTGGCTATGA AGGTGCTACC
    AAGACACGCG GTTTTAAAAA AGAAACCATG CGAGATTCCC TTCCAGCCGA GAGAAAAAGT
    AAAGGTAGCG GCGGTTCGAA GCCCGCTGTG CCGAGGTCTG CGTCTCAGCC AGGATGGAGT
    CAACGGCGTC TGCCTTCAAT GGACGCTGAG GATTTCGAGA CCATTCCGTC TAGTGCTGAA
    GAGCTGTCTA TTTCCTCTGA TGGAGAGGAT GAAGGACACA ACAAAAACGG GGATAATGAC
    CGCTATCAGG TGCAGTTGAC TTACGAGTCT TGCACTGCTC AGGATCTTTC TGTAGAAGCT
    GGAGATTTTG TACAATTCCT GGAAGAGTCA GAGAACGGCC TCTGGCTAGT CAAGAACCTT
    AGAACTCAGA AGACAGGACT GGTGCCGCCC ACTGTCCTGC AGTCCACAGC AGAGGAACTA
    GACCTTTACA GTAACTCCGA TATTTTCAGT GACCTCTGCA CCGCCGCACT CACCGATACG
    GAGGACGATA TCGGTGGGAC AAATCCTGCT CAGGACGGCA GATTTACATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54311
    Clone ID Related Accession (Same CDS sequence) XM_017358284.1
    Accession Version XM_017358284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGAA
    ACCATGCGAG ATTCCCTTCC AGCCGAGAGA AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC
    GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA TGGAGTCAAC GGCGTCTGCC TTCAATGGAC
    GCTGAGGATT TCGAGACCAT TCCGTCTAGT GCTGAAGAGC TGTCTATTTC CTCTGATGGA
    GAGGATGAAG GACACAACAA AAACGGGGAT AATGACCGCT ATCAGGTGCA GTTGACTTAC
    GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA GAAGCTGGAG ATTTTGTACA ATTCCTGGAA
    GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG AACCTTAGAA CTCAGAAGAC AGGACTGGTG
    CCGCCCACTG TCCTGCAGTC CACAGCAGAG GAACTAGACC TTTACAGTAA CTCCGATATT
    TTCAGTGACC TCTGCACCGC CGCACTCACC GATACGGAGG ACGATATCGG TGGGACAAAT
    CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213773.1
    CDS327..4076
    Translation

    Target ORF information:

    RefSeq Version XM_017358284.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGAA
    ACCATGCGAG ATTCCCTTCC AGCCGAGAGA AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC
    GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA TGGAGTCAAC GGCGTCTGCC TTCAATGGAC
    GCTGAGGATT TCGAGACCAT TCCGTCTAGT GCTGAAGAGC TGTCTATTTC CTCTGATGGA
    GAGGATGAAG GACACAACAA AAACGGGGAT AATGACCGCT ATCAGGTGCA GTTGACTTAC
    GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA GAAGCTGGAG ATTTTGTACA ATTCCTGGAA
    GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG AACCTTAGAA CTCAGAAGAC AGGACTGGTG
    CCGCCCACTG TCCTGCAGTC CACAGCAGAG GAACTAGACC TTTACAGTAA CTCCGATATT
    TTCAGTGACC TCTGCACCGC CGCACTCACC GATACGGAGG ACGATATCGG TGGGACAAAT
    CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54312
    Clone ID Related Accession (Same CDS sequence) XM_017358285.1
    Accession Version XM_017358285.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3591bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGGT
    GCTACCAAGA CACGCGGTTT TAAAAAAGAA ACCATGCGAG ATTCCCTTCC AGCCGAGAGA
    AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA
    TGGAGTCAAC GGCGTCTGCC TTCAATGGAC GCTGAGGATT TCGAGACCAT TCCGTCTAGT
    GCTGAAGAGC TGTCTATTTC CTCTGATGGA GAGGATGAAG GACACAACAA AAACGGGGAT
    AATGACCGCT ATCAGGTGCA GTTGACTTAC GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA
    GAAGCTGGAG ATTTTGTACA ATTCCTGGAA GAGTCAGAGA ACGGCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213774.1
    CDS324..3914
    Translation

    Target ORF information:

    RefSeq Version XM_017358285.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGGT
    GCTACCAAGA CACGCGGTTT TAAAAAAGAA ACCATGCGAG ATTCCCTTCC AGCCGAGAGA
    AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA
    TGGAGTCAAC GGCGTCTGCC TTCAATGGAC GCTGAGGATT TCGAGACCAT TCCGTCTAGT
    GCTGAAGAGC TGTCTATTTC CTCTGATGGA GAGGATGAAG GACACAACAA AAACGGGGAT
    AATGACCGCT ATCAGGTGCA GTTGACTTAC GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA
    GAAGCTGGAG ATTTTGTACA ATTCCTGGAA GAGTCAGAGA ACGGCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54313
    Clone ID Related Accession (Same CDS sequence) XM_017358286.1
    Accession Version XM_017358286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3408bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGGATG GAGTCAACGG
    CGTCTGCCTT CAATGGACGC TGAGGATTTC GAGACCATTC CGTCTAGTGC TGAAGAGCTG
    TCTATTTCCT CTGATGGAGA GGATGAAGGA CACAACAAAA ACGGGGATAA TGACCGCTAT
    CAGGTGCAGT TGACTTACGA GTCTTGCACT GCTCAGGATC TTTCTGTAGA AGCTGGAGAT
    TTTGTACAAT TCCTGGAAGA GTCAGAGAAC GGCCTCTGGC TAGTCAAGAA CCTTAGAACT
    CAGAAGACAG GACTGGTGCC GCCCACTGTC CTGCAGTCCA CAGCAGAGGA ACTAGACCTT
    TACAGTAACT CCGATATTTT CAGTGACCTC TGCACCGCCG CACTCACCGA TACGGAGGAC
    GATATCGGTG GGACAAATCC TGCTCAGGAC GGCAGATTTA CATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213775.1
    CDS322..3729
    Translation

    Target ORF information:

    RefSeq Version XM_017358286.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGGATG GAGTCAACGG
    CGTCTGCCTT CAATGGACGC TGAGGATTTC GAGACCATTC CGTCTAGTGC TGAAGAGCTG
    TCTATTTCCT CTGATGGAGA GGATGAAGGA CACAACAAAA ACGGGGATAA TGACCGCTAT
    CAGGTGCAGT TGACTTACGA GTCTTGCACT GCTCAGGATC TTTCTGTAGA AGCTGGAGAT
    TTTGTACAAT TCCTGGAAGA GTCAGAGAAC GGCCTCTGGC TAGTCAAGAA CCTTAGAACT
    CAGAAGACAG GACTGGTGCC GCCCACTGTC CTGCAGTCCA CAGCAGAGGA ACTAGACCTT
    TACAGTAACT CCGATATTTT CAGTGACCTC TGCACCGCCG CACTCACCGA TACGGAGGAC
    GATATCGGTG GGACAAATCC TGCTCAGGAC GGCAGATTTA CATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54314
    Clone ID Related Accession (Same CDS sequence) XM_017358287.1
    Accession Version XM_017358287.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3093bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGAGCC ATGGTCATCA
    AGCTCTCCGG ATTTGCAGTC ATTTGCAGGG AAGACTTTTT ATCCTAAAGC TGTTGTCTGT
    CATCTCTTTC CAACTTATTT TTTGATCATG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213776.1
    CDS322..3414
    Translation

    Target ORF information:

    RefSeq Version XM_017358287.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358287.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGAGCC ATGGTCATCA
    AGCTCTCCGG ATTTGCAGTC ATTTGCAGGG AAGACTTTTT ATCCTAAAGC TGTTGTCTGT
    CATCTCTTTC CAACTTATTT TTTGATCATG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48787
    Clone ID Related Accession (Same CDS sequence) XM_009305853.3 , XM_009305853.2
    Accession Version XM_009305853.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGGT
    GCTACCAAGA CACGCGGTTT TAAAAAAGAA ACCATGCGAG ATTCCCTTCC AGCCGAGAGA
    AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA
    TGGAGTCAAC GGCGTCTGCC TTCAATGGAC GCTGAGGATT TCGAGACCAT TCCGTCTAGT
    GCTGAAGAGC TGTCTATTTC CTCTGATGGA GAGGATGAAG GACACAACAA AAACGGGGAT
    AATGACCGCT ATCAGGTGCA GTTGACTTAC GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA
    GAAGCTGGAG ATTTTGTACA ATTCCTGGAA GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG
    AACCTTAGAA CTCAGAAGAC AGGACTGGTG CCGCCCACTG TCCTGCAGTC CACAGCAGAG
    GAACTAGACC TTTACAGTAA CTCCGATATT TTCAGTGACC TCTGCACCGC CGCACTCACC
    GATACGGAGG ACGATATCGG TGGGACAAAT CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304128.1
    CDS384..4163
    Translation

    Target ORF information:

    RefSeq Version XM_009305853.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305853.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGGT
    GCTACCAAGA CACGCGGTTT TAAAAAAGAA ACCATGCGAG ATTCCCTTCC AGCCGAGAGA
    AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA
    TGGAGTCAAC GGCGTCTGCC TTCAATGGAC GCTGAGGATT TCGAGACCAT TCCGTCTAGT
    GCTGAAGAGC TGTCTATTTC CTCTGATGGA GAGGATGAAG GACACAACAA AAACGGGGAT
    AATGACCGCT ATCAGGTGCA GTTGACTTAC GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA
    GAAGCTGGAG ATTTTGTACA ATTCCTGGAA GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG
    AACCTTAGAA CTCAGAAGAC AGGACTGGTG CCGCCCACTG TCCTGCAGTC CACAGCAGAG
    GAACTAGACC TTTACAGTAA CTCCGATATT TTCAGTGACC TCTGCACCGC CGCACTCACC
    GATACGGAGG ACGATATCGG TGGGACAAAT CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48787
    Clone ID Related Accession (Same CDS sequence) XM_009305853.3 , XM_009305853.2
    Accession Version XM_009305853.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGGT
    GCTACCAAGA CACGCGGTTT TAAAAAAGAA ACCATGCGAG ATTCCCTTCC AGCCGAGAGA
    AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA
    TGGAGTCAAC GGCGTCTGCC TTCAATGGAC GCTGAGGATT TCGAGACCAT TCCGTCTAGT
    GCTGAAGAGC TGTCTATTTC CTCTGATGGA GAGGATGAAG GACACAACAA AAACGGGGAT
    AATGACCGCT ATCAGGTGCA GTTGACTTAC GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA
    GAAGCTGGAG ATTTTGTACA ATTCCTGGAA GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG
    AACCTTAGAA CTCAGAAGAC AGGACTGGTG CCGCCCACTG TCCTGCAGTC CACAGCAGAG
    GAACTAGACC TTTACAGTAA CTCCGATATT TTCAGTGACC TCTGCACCGC CGCACTCACC
    GATACGGAGG ACGATATCGG TGGGACAAAT CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304128.1
    CDS328..4107
    Translation

    Target ORF information:

    RefSeq Version XM_009305853.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305853.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTCTGCA GGTTGGAGGA GGAAGAGGAG ATGTCTCTAC TGGAGCTTCA GCAGAGGGTG 
    GACTGTATCA TCACCCATGT TGATGAGATT ATGCAGCAGG AGATCAGGCC GTTGCTGGCT
    GTCGACATCA TTGAACAACT TCACAGGCAG TTTGCTCTTC TTTCAGGAGG AAGAGGACAA
    GAGGGTTCTC CCATCATCAC CTTCCCAGAG TTCTCAGGTT TTAATGAGGT TTCAGATGAG
    GATTTTATTA ATGTTGTCAC TTACCTGACC AGCATACCTA GCATGGATGC TGCTAGTATC
    GGCTTCATCA TCATCTTGGA CCGAAGGAAG GATAAATGGA ACTCAGCAAT CGCTTCTCTG
    GCACGCATAG CGGGCTCCTT TCCAGGAAAC CTACAGCTAG TTCTAGTTCT GAAACCTTCG
    CGCTTCCTCC AGAGGGCCAT CGCTGACGTC AGTATGAGGT TACACAGAGA TGACTTCAAA
    TTGAAGGTAC CGATTGTTAT GCTAAATTCC CTGTCTGACT TGCATGGCTA CATTGATAAA
    GGCCAGTTGA CCTGTGACCT TGGGGGAACC CTGGATTATT GCCACAGTCA ATGGATTCAT
    CATCGCACAG CCATTGAGAA CTTTGCGCTG ACAGTGAAGA GCACAGCACA GATGCTGCAG
    CAGTTCGGAA CAGACCTGGC AGAGACTGAA CTTCCCAATG ACGTGCAGTG CACCAAAGAT
    TTGCTCATCA CACACACTGA AAAACACGAT AAGCTCAAGG ATGAACTAAA ATTGGCTGTA
    AAACAAGGGA ATACTCTGCT GTCCTGTATT AAAGACCAGG CCACCAAAGC CGAGAGCCAC
    ATTTTGAACC CAGACGAGGT TGAGAACCAA ACAACCGTTG AGAGGTTGTT AGCGCAGCTT
    GATGAGACGG AGAATGCATT TGAACAATTC TGGTCAAGAC ATCACCTCAA GTTAGAGCAG
    TGCCTACAGT TACGACACTT TGAGCAGGAC TTCAAAGAGG TGAAAGTGTC ACTGGACGGC
    CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT ACCGGGGACT GCGTGGCTCG TGTGGAACAG
    CTATTAGGAG AACTGAAAAC CTTGGAGGAT AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG
    CTTCAAGCTC AGCATGGGGA TCAGCTCATT CAGAGTAATC ACTACGCCAT CGACTCCATC
    CGCCCCAAGT GTGTGGAACT GCGTCGGATC TGTGACGACT TCAGCAACGA GGCCAAAAAG
    AAGCGTGACG TTCTGACCAA ATCACTTGAA ATCCTCAAAC GAATTGACGA GGTGAACCAG
    TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC TCTCAGGCTG TAGACAGATG TCAAACACAG
    GAAGGTGCAG AAGCAGCACT GCAGGACATC GAGAGGTACA TGAAAACAGC CAAAGAGCAG
    CAGCTCAGTC ACCTCAAAGA ACTCAACAAC CAGTATGAGG TCGTCCTCTC AGAGCTGCTC
    AAGAGCAAAG TTCAAACTGC CTTGAAAACG CTGAACGATG TCCAAGAGAT GTTTGAGAAA
    AGACATGCAA GTCTGAAAAA GCTTTCCACG AAGCAAACAA GGCCTGTGCA ACCTGTTGCA
    CCCCGACCAG AATCTTCACC AAAACGCCCC TCACCTAAAA TCCCACATCC CACTCCTCCA
    ACATCACACC GCAAAACTAC AGATAACTCC AGCACCAATA GACAGCCCAG TGATGCAGAA
    CTGGCAAAAA GAAAAAACAT TCGCAAAGCC AAAGGTGGCA TTAAGATTGA GGTCATGCAT
    GAAGGAGGTC AAGGAGGTTC AAGTCATATT TTAATGAACA ATGAAACTGA AGAGTCGCTT
    TCAAACAGAC GCAGTCACAT TATGAATGAA CTAATTGAGA CGGAAAGGCT GTATGTGGAA
    GAGCTGCAGA GCATCATAGA GGGCTATGCC GCGGCACTCG ATGACCCAGA ACTCATCTAT
    CTGATCCCAC CATCCCTGGA AAACAAGAAG GAAGTTCTCT TTGGAAATCT GCCAGAAATT
    TACCAATTCC ACCAGAAGAT ATTTCTCAAA GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG
    TTGGTGGGAA CATGTTTTTT GAAGCGGAAA GAAGAGTTGC AGATTTATGA GAAATATTGT
    CAGAATAAGC CACGCTCTGA AGCTTTGTGG CGGCAATGCG GGGACAGTAT GTTCTTTCAG
    GAGTGTCAGA AAAGGTTGGA CCACAAGCTT TCATTAGACG CGTATCTGCT AAAGCCTGTG
    CAGAGAATCA CCAAATACCA GCTTATGTTA AAGGAGATGC TGAAATGCAG TAAGAACCCA
    GAAGGCACCG CTGAGCTGGA GGAAGCTCAG GCCACAGTGC TGGACATCAT CAAATCGGTC
    AACGACTCCA TGCATCAGAT CGCCATCACT GGTTTTGAGG GCAGTTTGAA TGATCTGGGG
    AAACTGCTTA TGCAGGGGTC GTTTAACGTC TGGACAGACC ATAAGAAAGG CCATAGTAAG
    GTGAAGGACT TGGCACGCTT CAAACCCATG CAGAGACATC TCTTCCTCTA CCACAAAATG
    ATATTGTTCT GCAAGAAACG TGAGGAGATG ACTGATGGCC ATGAAAAATC CCCTTCCTAC
    AGTTTCAAGC ACTCATTAAA GATGGGCTCA GTGGGAATCA CAGAAAATGT AAAGGGAGAC
    ATCAAGAAGT TTGAAATCTG GTACAATGGC AGAGAAGAAG TTTACATCAT TCAGGCCCCG
    TCCATGGACG TGAAGAACAC GTGGGTATCA GAAATCCGCA AAGTCTTAAC AAGCCAGCTG
    GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT CAGAAGATTA CAGAGAACAT CTACCATGCC
    CCCATGAGAA ATATGCGTAA AACGGCCCTC AGACAGTCCG ACTCTTCCAG TCCTGAAACA
    GGCTACAGAC GCAGTGACTC GGGTCCAAAT ATGCGTCAGA AACGAGCTGA CACTTCATCC
    AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT AGAAAGCGTT TCACCTTACA GGGCCTGTCA
    AACCGCAGAT CTCTGCCCGC AGAACCCGCA CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT
    TCTTTAGCCT CAACTGTCGG CTCTGCCACT GCTCCACGCA CTAAAGCTCC TCTCTCCTCT
    AGCCTTAAGA GTAAGAGGCA TCAGATCAAG AGCGACCCTA CACCATTTGG CTATGAAGGT
    GCTACCAAGA CACGCGGTTT TAAAAAAGAA ACCATGCGAG ATTCCCTTCC AGCCGAGAGA
    AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA
    TGGAGTCAAC GGCGTCTGCC TTCAATGGAC GCTGAGGATT TCGAGACCAT TCCGTCTAGT
    GCTGAAGAGC TGTCTATTTC CTCTGATGGA GAGGATGAAG GACACAACAA AAACGGGGAT
    AATGACCGCT ATCAGGTGCA GTTGACTTAC GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA
    GAAGCTGGAG ATTTTGTACA ATTCCTGGAA GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG
    AACCTTAGAA CTCAGAAGAC AGGACTGGTG CCGCCCACTG TCCTGCAGTC CACAGCAGAG
    GAACTAGACC TTTACAGTAA CTCCGATATT TTCAGTGACC TCTGCACCGC CGCACTCACC
    GATACGGAGG ACGATATCGG TGGGACAAAT CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48788
    Clone ID Related Accession (Same CDS sequence) XM_009305854.3 , XM_009305854.2
    Accession Version XM_009305854.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTTCAG TTATGTATGA GATTATGCAG CAGGAGATCA GGCCGTTGCT GGCTGTCGAC 
    ATCATTGAAC AACTTCACAG GCAGTTTGCT CTTCTTTCAG GAGGAAGAGG ACAAGAGGGT
    TCTCCCATCA TCACCTTCCC AGAGTTCTCA GGTTTTAATG AGGTTTCAGA TGAGGATTTT
    ATTAATGTTG TCACTTACCT GACCAGCATA CCTAGCATGG ATGCTGCTAG TATCGGCTTC
    ATCATCATCT TGGACCGAAG GAAGGATAAA TGGAACTCAG CAATCGCTTC TCTGGCACGC
    ATAGCGGGCT CCTTTCCAGG AAACCTACAG CTAGTTCTAG TTCTGAAACC TTCGCGCTTC
    CTCCAGAGGG CCATCGCTGA CGTCAGTATG AGGTTACACA GAGATGACTT CAAATTGAAG
    GTACCGATTG TTATGCTAAA TTCCCTGTCT GACTTGCATG GCTACATTGA TAAAGGCCAG
    TTGACCTGTG ACCTTGGGGG AACCCTGGAT TATTGCCACA GTCAATGGAT TCATCATCGC
    ACAGCCATTG AGAACTTTGC GCTGACAGTG AAGAGCACAG CACAGATGCT GCAGCAGTTC
    GGAACAGACC TGGCAGAGAC TGAACTTCCC AATGACGTGC AGTGCACCAA AGATTTGCTC
    ATCACACACA CTGAAAAACA CGATAAGCTC AAGGATGAAC TAAAATTGGC TGTAAAACAA
    GGGAATACTC TGCTGTCCTG TATTAAAGAC CAGGCCACCA AAGCCGAGAG CCACATTTTG
    AACCCAGACG AGGTTGAGAA CCAAACAACC GTTGAGAGGT TGTTAGCGCA GCTTGATGAG
    ACGGAGAATG CATTTGAACA ATTCTGGTCA AGACATCACC TCAAGTTAGA GCAGTGCCTA
    CAGTTACGAC ACTTTGAGCA GGACTTCAAA GAGGTGAAAG TGTCACTGGA CGGCCTCTCT
    GAAGCTTTGC TGGGACTGTC AGATACCGGG GACTGCGTGG CTCGTGTGGA ACAGCTATTA
    GGAGAACTGA AAACCTTGGA GGATAAAGCT CAGGAGTGTT TGGAGAAAGC TCAGCTTCAA
    GCTCAGCATG GGGATCAGCT CATTCAGAGT AATCACTACG CCATCGACTC CATCCGCCCC
    AAGTGTGTGG AACTGCGTCG GATCTGTGAC GACTTCAGCA ACGAGGCCAA AAAGAAGCGT
    GACGTTCTGA CCAAATCACT TGAAATCCTC AAACGAATTG ACGAGGTGAA CCAGTGGTGT
    GAAAGCGGAG TGTATCTGCT GGCCTCTCAG GCTGTAGACA GATGTCAAAC ACAGGAAGGT
    GCAGAAGCAG CACTGCAGGA CATCGAGAGG TACATGAAAA CAGCCAAAGA GCAGCAGCTC
    AGTCACCTCA AAGAACTCAA CAACCAGTAT GAGGTCGTCC TCTCAGAGCT GCTCAAGAGC
    AAAGTTCAAA CTGCCTTGAA AACGCTGAAC GATGTCCAAG AGATGTTTGA GAAAAGACAT
    GCAAGTCTGA AAAAGCTTTC CACGAAGCAA ACAAGGCCTG TGCAACCTGT TGCACCCCGA
    CCAGAATCTT CACCAAAACG CCCCTCACCT AAAATCCCAC ATCCCACTCC TCCAACATCA
    CACCGCAAAA CTACAGATAA CTCCAGCACC AATAGACAGC CCAGTGATGC AGAACTGGCA
    AAAAGAAAAA ACATTCGCAA AGCCAAAGGT GGCATTAAGA TTGAGGTCAT GCATGAAGGA
    GGTCAAGGAG GTTCAAGTCA TATTTTAATG AACAATGAAA CTGAAGAGTC GCTTTCAAAC
    AGACGCAGTC ACATTATGAA TGAACTAATT GAGACGGAAA GGCTGTATGT GGAAGAGCTG
    CAGAGCATCA TAGAGGGCTA TGCCGCGGCA CTCGATGACC CAGAACTCAT CTATCTGATC
    CCACCATCCC TGGAAAACAA GAAGGAAGTT CTCTTTGGAA ATCTGCCAGA AATTTACCAA
    TTCCACCAGA AGATATTTCT CAAAGAGCTG GAAAATTCTG CCGAAAAGCC GGAGTTGGTG
    GGAACATGTT TTTTGAAGCG GAAAGAAGAG TTGCAGATTT ATGAGAAATA TTGTCAGAAT
    AAGCCACGCT CTGAAGCTTT GTGGCGGCAA TGCGGGGACA GTATGTTCTT TCAGGAGTGT
    CAGAAAAGGT TGGACCACAA GCTTTCATTA GACGCGTATC TGCTAAAGCC TGTGCAGAGA
    ATCACCAAAT ACCAGCTTAT GTTAAAGGAG ATGCTGAAAT GCAGTAAGAA CCCAGAAGGC
    ACCGCTGAGC TGGAGGAAGC TCAGGCCACA GTGCTGGACA TCATCAAATC GGTCAACGAC
    TCCATGCATC AGATCGCCAT CACTGGTTTT GAGGGCAGTT TGAATGATCT GGGGAAACTG
    CTTATGCAGG GGTCGTTTAA CGTCTGGACA GACCATAAGA AAGGCCATAG TAAGGTGAAG
    GACTTGGCAC GCTTCAAACC CATGCAGAGA CATCTCTTCC TCTACCACAA AATGATATTG
    TTCTGCAAGA AACGTGAGGA GATGACTGAT GGCCATGAAA AATCCCCTTC CTACAGTTTC
    AAGCACTCAT TAAAGATGGG CTCAGTGGGA ATCACAGAAA ATGTAAAGGG AGACATCAAG
    AAGTTTGAAA TCTGGTACAA TGGCAGAGAA GAAGTTTACA TCATTCAGGC CCCGTCCATG
    GACGTGAAGA ACACGTGGGT ATCAGAAATC CGCAAAGTCT TAACAAGCCA GCTGGAAGCC
    TGCCGAGAAG CCAGTCAGCT CCATCAGAAG ATTACAGAGA ACATCTACCA TGCCCCCATG
    AGAAATATGC GTAAAACGGC CCTCAGACAG TCCGACTCTT CCAGTCCTGA AACAGGCTAC
    AGACGCAGTG ACTCGGGTCC AAATATGCGT CAGAAACGAG CTGACACTTC ATCCAGCCTC
    GGCTCAGAGC GAGCGGCTCT TCCTAGAAAG CGTTTCACCT TACAGGGCCT GTCAAACCGC
    AGATCTCTGC CCGCAGAACC CGCACCTAAG GAGACAGAAG TCACTCGCCG CTTTTCTTTA
    GCCTCAACTG TCGGCTCTGC CACTGCTCCA CGCACTAAAG CTCCTCTCTC CTCTAGCCTT
    AAGAGTAAGA GGCATCAGAT CAAGAGCGAC CCTACACCAT TTGGCTATGA AGGTGCTACC
    AAGACACGCG GTTTTAAAAA AGAAACCATG CGAGATTCCC TTCCAGCCGA GAGAAAAAGT
    AAAGGTAGCG GCGGTTCGAA GCCCGCTGTG CCGAGGTCTG CGTCTCAGCC AGGATGGAGT
    CAACGGCGTC TGCCTTCAAT GGACGCTGAG GATTTCGAGA CCATTCCGTC TAGTGCTGAA
    GAGCTGTCTA TTTCCTCTGA TGGAGAGGAT GAAGGACACA ACAAAAACGG GGATAATGAC
    CGCTATCAGG TGCAGTTGAC TTACGAGTCT TGCACTGCTC AGGATCTTTC TGTAGAAGCT
    GGAGATTTTG TACAATTCCT GGAAGAGTCA GAGAACGGCC TCTGGCTAGT CAAGAACCTT
    AGAACTCAGA AGACAGGACT GGTGCCGCCC ACTGTCCTGC AGTCCACAGC AGAGGAACTA
    GACCTTTACA GTAACTCCGA TATTTTCAGT GACCTCTGCA CCGCCGCACT CACCGATACG
    GAGGACGATA TCGGTGGGAC AAATCCTGCT CAGGACGGCA GATTTACATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304129.1
    CDS762..4475
    Translation

    Target ORF information:

    RefSeq Version XM_009305854.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305854.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTTCAG TTATGTATGA GATTATGCAG CAGGAGATCA GGCCGTTGCT GGCTGTCGAC 
    ATCATTGAAC AACTTCACAG GCAGTTTGCT CTTCTTTCAG GAGGAAGAGG ACAAGAGGGT
    TCTCCCATCA TCACCTTCCC AGAGTTCTCA GGTTTTAATG AGGTTTCAGA TGAGGATTTT
    ATTAATGTTG TCACTTACCT GACCAGCATA CCTAGCATGG ATGCTGCTAG TATCGGCTTC
    ATCATCATCT TGGACCGAAG GAAGGATAAA TGGAACTCAG CAATCGCTTC TCTGGCACGC
    ATAGCGGGCT CCTTTCCAGG AAACCTACAG CTAGTTCTAG TTCTGAAACC TTCGCGCTTC
    CTCCAGAGGG CCATCGCTGA CGTCAGTATG AGGTTACACA GAGATGACTT CAAATTGAAG
    GTACCGATTG TTATGCTAAA TTCCCTGTCT GACTTGCATG GCTACATTGA TAAAGGCCAG
    TTGACCTGTG ACCTTGGGGG AACCCTGGAT TATTGCCACA GTCAATGGAT TCATCATCGC
    ACAGCCATTG AGAACTTTGC GCTGACAGTG AAGAGCACAG CACAGATGCT GCAGCAGTTC
    GGAACAGACC TGGCAGAGAC TGAACTTCCC AATGACGTGC AGTGCACCAA AGATTTGCTC
    ATCACACACA CTGAAAAACA CGATAAGCTC AAGGATGAAC TAAAATTGGC TGTAAAACAA
    GGGAATACTC TGCTGTCCTG TATTAAAGAC CAGGCCACCA AAGCCGAGAG CCACATTTTG
    AACCCAGACG AGGTTGAGAA CCAAACAACC GTTGAGAGGT TGTTAGCGCA GCTTGATGAG
    ACGGAGAATG CATTTGAACA ATTCTGGTCA AGACATCACC TCAAGTTAGA GCAGTGCCTA
    CAGTTACGAC ACTTTGAGCA GGACTTCAAA GAGGTGAAAG TGTCACTGGA CGGCCTCTCT
    GAAGCTTTGC TGGGACTGTC AGATACCGGG GACTGCGTGG CTCGTGTGGA ACAGCTATTA
    GGAGAACTGA AAACCTTGGA GGATAAAGCT CAGGAGTGTT TGGAGAAAGC TCAGCTTCAA
    GCTCAGCATG GGGATCAGCT CATTCAGAGT AATCACTACG CCATCGACTC CATCCGCCCC
    AAGTGTGTGG AACTGCGTCG GATCTGTGAC GACTTCAGCA ACGAGGCCAA AAAGAAGCGT
    GACGTTCTGA CCAAATCACT TGAAATCCTC AAACGAATTG ACGAGGTGAA CCAGTGGTGT
    GAAAGCGGAG TGTATCTGCT GGCCTCTCAG GCTGTAGACA GATGTCAAAC ACAGGAAGGT
    GCAGAAGCAG CACTGCAGGA CATCGAGAGG TACATGAAAA CAGCCAAAGA GCAGCAGCTC
    AGTCACCTCA AAGAACTCAA CAACCAGTAT GAGGTCGTCC TCTCAGAGCT GCTCAAGAGC
    AAAGTTCAAA CTGCCTTGAA AACGCTGAAC GATGTCCAAG AGATGTTTGA GAAAAGACAT
    GCAAGTCTGA AAAAGCTTTC CACGAAGCAA ACAAGGCCTG TGCAACCTGT TGCACCCCGA
    CCAGAATCTT CACCAAAACG CCCCTCACCT AAAATCCCAC ATCCCACTCC TCCAACATCA
    CACCGCAAAA CTACAGATAA CTCCAGCACC AATAGACAGC CCAGTGATGC AGAACTGGCA
    AAAAGAAAAA ACATTCGCAA AGCCAAAGGT GGCATTAAGA TTGAGGTCAT GCATGAAGGA
    GGTCAAGGAG GTTCAAGTCA TATTTTAATG AACAATGAAA CTGAAGAGTC GCTTTCAAAC
    AGACGCAGTC ACATTATGAA TGAACTAATT GAGACGGAAA GGCTGTATGT GGAAGAGCTG
    CAGAGCATCA TAGAGGGCTA TGCCGCGGCA CTCGATGACC CAGAACTCAT CTATCTGATC
    CCACCATCCC TGGAAAACAA GAAGGAAGTT CTCTTTGGAA ATCTGCCAGA AATTTACCAA
    TTCCACCAGA AGATATTTCT CAAAGAGCTG GAAAATTCTG CCGAAAAGCC GGAGTTGGTG
    GGAACATGTT TTTTGAAGCG GAAAGAAGAG TTGCAGATTT ATGAGAAATA TTGTCAGAAT
    AAGCCACGCT CTGAAGCTTT GTGGCGGCAA TGCGGGGACA GTATGTTCTT TCAGGAGTGT
    CAGAAAAGGT TGGACCACAA GCTTTCATTA GACGCGTATC TGCTAAAGCC TGTGCAGAGA
    ATCACCAAAT ACCAGCTTAT GTTAAAGGAG ATGCTGAAAT GCAGTAAGAA CCCAGAAGGC
    ACCGCTGAGC TGGAGGAAGC TCAGGCCACA GTGCTGGACA TCATCAAATC GGTCAACGAC
    TCCATGCATC AGATCGCCAT CACTGGTTTT GAGGGCAGTT TGAATGATCT GGGGAAACTG
    CTTATGCAGG GGTCGTTTAA CGTCTGGACA GACCATAAGA AAGGCCATAG TAAGGTGAAG
    GACTTGGCAC GCTTCAAACC CATGCAGAGA CATCTCTTCC TCTACCACAA AATGATATTG
    TTCTGCAAGA AACGTGAGGA GATGACTGAT GGCCATGAAA AATCCCCTTC CTACAGTTTC
    AAGCACTCAT TAAAGATGGG CTCAGTGGGA ATCACAGAAA ATGTAAAGGG AGACATCAAG
    AAGTTTGAAA TCTGGTACAA TGGCAGAGAA GAAGTTTACA TCATTCAGGC CCCGTCCATG
    GACGTGAAGA ACACGTGGGT ATCAGAAATC CGCAAAGTCT TAACAAGCCA GCTGGAAGCC
    TGCCGAGAAG CCAGTCAGCT CCATCAGAAG ATTACAGAGA ACATCTACCA TGCCCCCATG
    AGAAATATGC GTAAAACGGC CCTCAGACAG TCCGACTCTT CCAGTCCTGA AACAGGCTAC
    AGACGCAGTG ACTCGGGTCC AAATATGCGT CAGAAACGAG CTGACACTTC ATCCAGCCTC
    GGCTCAGAGC GAGCGGCTCT TCCTAGAAAG CGTTTCACCT TACAGGGCCT GTCAAACCGC
    AGATCTCTGC CCGCAGAACC CGCACCTAAG GAGACAGAAG TCACTCGCCG CTTTTCTTTA
    GCCTCAACTG TCGGCTCTGC CACTGCTCCA CGCACTAAAG CTCCTCTCTC CTCTAGCCTT
    AAGAGTAAGA GGCATCAGAT CAAGAGCGAC CCTACACCAT TTGGCTATGA AGGTGCTACC
    AAGACACGCG GTTTTAAAAA AGAAACCATG CGAGATTCCC TTCCAGCCGA GAGAAAAAGT
    AAAGGTAGCG GCGGTTCGAA GCCCGCTGTG CCGAGGTCTG CGTCTCAGCC AGGATGGAGT
    CAACGGCGTC TGCCTTCAAT GGACGCTGAG GATTTCGAGA CCATTCCGTC TAGTGCTGAA
    GAGCTGTCTA TTTCCTCTGA TGGAGAGGAT GAAGGACACA ACAAAAACGG GGATAATGAC
    CGCTATCAGG TGCAGTTGAC TTACGAGTCT TGCACTGCTC AGGATCTTTC TGTAGAAGCT
    GGAGATTTTG TACAATTCCT GGAAGAGTCA GAGAACGGCC TCTGGCTAGT CAAGAACCTT
    AGAACTCAGA AGACAGGACT GGTGCCGCCC ACTGTCCTGC AGTCCACAGC AGAGGAACTA
    GACCTTTACA GTAACTCCGA TATTTTCAGT GACCTCTGCA CCGCCGCACT CACCGATACG
    GAGGACGATA TCGGTGGGAC AAATCCTGCT CAGGACGGCA GATTTACATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48788
    Clone ID Related Accession (Same CDS sequence) XM_009305854.3 , XM_009305854.2
    Accession Version XM_009305854.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTTCAG TTATGTATGA GATTATGCAG CAGGAGATCA GGCCGTTGCT GGCTGTCGAC 
    ATCATTGAAC AACTTCACAG GCAGTTTGCT CTTCTTTCAG GAGGAAGAGG ACAAGAGGGT
    TCTCCCATCA TCACCTTCCC AGAGTTCTCA GGTTTTAATG AGGTTTCAGA TGAGGATTTT
    ATTAATGTTG TCACTTACCT GACCAGCATA CCTAGCATGG ATGCTGCTAG TATCGGCTTC
    ATCATCATCT TGGACCGAAG GAAGGATAAA TGGAACTCAG CAATCGCTTC TCTGGCACGC
    ATAGCGGGCT CCTTTCCAGG AAACCTACAG CTAGTTCTAG TTCTGAAACC TTCGCGCTTC
    CTCCAGAGGG CCATCGCTGA CGTCAGTATG AGGTTACACA GAGATGACTT CAAATTGAAG
    GTACCGATTG TTATGCTAAA TTCCCTGTCT GACTTGCATG GCTACATTGA TAAAGGCCAG
    TTGACCTGTG ACCTTGGGGG AACCCTGGAT TATTGCCACA GTCAATGGAT TCATCATCGC
    ACAGCCATTG AGAACTTTGC GCTGACAGTG AAGAGCACAG CACAGATGCT GCAGCAGTTC
    GGAACAGACC TGGCAGAGAC TGAACTTCCC AATGACGTGC AGTGCACCAA AGATTTGCTC
    ATCACACACA CTGAAAAACA CGATAAGCTC AAGGATGAAC TAAAATTGGC TGTAAAACAA
    GGGAATACTC TGCTGTCCTG TATTAAAGAC CAGGCCACCA AAGCCGAGAG CCACATTTTG
    AACCCAGACG AGGTTGAGAA CCAAACAACC GTTGAGAGGT TGTTAGCGCA GCTTGATGAG
    ACGGAGAATG CATTTGAACA ATTCTGGTCA AGACATCACC TCAAGTTAGA GCAGTGCCTA
    CAGTTACGAC ACTTTGAGCA GGACTTCAAA GAGGTGAAAG TGTCACTGGA CGGCCTCTCT
    GAAGCTTTGC TGGGACTGTC AGATACCGGG GACTGCGTGG CTCGTGTGGA ACAGCTATTA
    GGAGAACTGA AAACCTTGGA GGATAAAGCT CAGGAGTGTT TGGAGAAAGC TCAGCTTCAA
    GCTCAGCATG GGGATCAGCT CATTCAGAGT AATCACTACG CCATCGACTC CATCCGCCCC
    AAGTGTGTGG AACTGCGTCG GATCTGTGAC GACTTCAGCA ACGAGGCCAA AAAGAAGCGT
    GACGTTCTGA CCAAATCACT TGAAATCCTC AAACGAATTG ACGAGGTGAA CCAGTGGTGT
    GAAAGCGGAG TGTATCTGCT GGCCTCTCAG GCTGTAGACA GATGTCAAAC ACAGGAAGGT
    GCAGAAGCAG CACTGCAGGA CATCGAGAGG TACATGAAAA CAGCCAAAGA GCAGCAGCTC
    AGTCACCTCA AAGAACTCAA CAACCAGTAT GAGGTCGTCC TCTCAGAGCT GCTCAAGAGC
    AAAGTTCAAA CTGCCTTGAA AACGCTGAAC GATGTCCAAG AGATGTTTGA GAAAAGACAT
    GCAAGTCTGA AAAAGCTTTC CACGAAGCAA ACAAGGCCTG TGCAACCTGT TGCACCCCGA
    CCAGAATCTT CACCAAAACG CCCCTCACCT AAAATCCCAC ATCCCACTCC TCCAACATCA
    CACCGCAAAA CTACAGATAA CTCCAGCACC AATAGACAGC CCAGTGATGC AGAACTGGCA
    AAAAGAAAAA ACATTCGCAA AGCCAAAGGT GGCATTAAGA TTGAGGTCAT GCATGAAGGA
    GGTCAAGGAG GTTCAAGTCA TATTTTAATG AACAATGAAA CTGAAGAGTC GCTTTCAAAC
    AGACGCAGTC ACATTATGAA TGAACTAATT GAGACGGAAA GGCTGTATGT GGAAGAGCTG
    CAGAGCATCA TAGAGGGCTA TGCCGCGGCA CTCGATGACC CAGAACTCAT CTATCTGATC
    CCACCATCCC TGGAAAACAA GAAGGAAGTT CTCTTTGGAA ATCTGCCAGA AATTTACCAA
    TTCCACCAGA AGATATTTCT CAAAGAGCTG GAAAATTCTG CCGAAAAGCC GGAGTTGGTG
    GGAACATGTT TTTTGAAGCG GAAAGAAGAG TTGCAGATTT ATGAGAAATA TTGTCAGAAT
    AAGCCACGCT CTGAAGCTTT GTGGCGGCAA TGCGGGGACA GTATGTTCTT TCAGGAGTGT
    CAGAAAAGGT TGGACCACAA GCTTTCATTA GACGCGTATC TGCTAAAGCC TGTGCAGAGA
    ATCACCAAAT ACCAGCTTAT GTTAAAGGAG ATGCTGAAAT GCAGTAAGAA CCCAGAAGGC
    ACCGCTGAGC TGGAGGAAGC TCAGGCCACA GTGCTGGACA TCATCAAATC GGTCAACGAC
    TCCATGCATC AGATCGCCAT CACTGGTTTT GAGGGCAGTT TGAATGATCT GGGGAAACTG
    CTTATGCAGG GGTCGTTTAA CGTCTGGACA GACCATAAGA AAGGCCATAG TAAGGTGAAG
    GACTTGGCAC GCTTCAAACC CATGCAGAGA CATCTCTTCC TCTACCACAA AATGATATTG
    TTCTGCAAGA AACGTGAGGA GATGACTGAT GGCCATGAAA AATCCCCTTC CTACAGTTTC
    AAGCACTCAT TAAAGATGGG CTCAGTGGGA ATCACAGAAA ATGTAAAGGG AGACATCAAG
    AAGTTTGAAA TCTGGTACAA TGGCAGAGAA GAAGTTTACA TCATTCAGGC CCCGTCCATG
    GACGTGAAGA ACACGTGGGT ATCAGAAATC CGCAAAGTCT TAACAAGCCA GCTGGAAGCC
    TGCCGAGAAG CCAGTCAGCT CCATCAGAAG ATTACAGAGA ACATCTACCA TGCCCCCATG
    AGAAATATGC GTAAAACGGC CCTCAGACAG TCCGACTCTT CCAGTCCTGA AACAGGCTAC
    AGACGCAGTG ACTCGGGTCC AAATATGCGT CAGAAACGAG CTGACACTTC ATCCAGCCTC
    GGCTCAGAGC GAGCGGCTCT TCCTAGAAAG CGTTTCACCT TACAGGGCCT GTCAAACCGC
    AGATCTCTGC CCGCAGAACC CGCACCTAAG GAGACAGAAG TCACTCGCCG CTTTTCTTTA
    GCCTCAACTG TCGGCTCTGC CACTGCTCCA CGCACTAAAG CTCCTCTCTC CTCTAGCCTT
    AAGAGTAAGA GGCATCAGAT CAAGAGCGAC CCTACACCAT TTGGCTATGA AGGTGCTACC
    AAGACACGCG GTTTTAAAAA AGAAACCATG CGAGATTCCC TTCCAGCCGA GAGAAAAAGT
    AAAGGTAGCG GCGGTTCGAA GCCCGCTGTG CCGAGGTCTG CGTCTCAGCC AGGATGGAGT
    CAACGGCGTC TGCCTTCAAT GGACGCTGAG GATTTCGAGA CCATTCCGTC TAGTGCTGAA
    GAGCTGTCTA TTTCCTCTGA TGGAGAGGAT GAAGGACACA ACAAAAACGG GGATAATGAC
    CGCTATCAGG TGCAGTTGAC TTACGAGTCT TGCACTGCTC AGGATCTTTC TGTAGAAGCT
    GGAGATTTTG TACAATTCCT GGAAGAGTCA GAGAACGGCC TCTGGCTAGT CAAGAACCTT
    AGAACTCAGA AGACAGGACT GGTGCCGCCC ACTGTCCTGC AGTCCACAGC AGAGGAACTA
    GACCTTTACA GTAACTCCGA TATTTTCAGT GACCTCTGCA CCGCCGCACT CACCGATACG
    GAGGACGATA TCGGTGGGAC AAATCCTGCT CAGGACGGCA GATTTACATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304129.1
    CDS395..4108
    Translation

    Target ORF information:

    RefSeq Version XM_009305854.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305854.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGGTTCAG TTATGTATGA GATTATGCAG CAGGAGATCA GGCCGTTGCT GGCTGTCGAC 
    ATCATTGAAC AACTTCACAG GCAGTTTGCT CTTCTTTCAG GAGGAAGAGG ACAAGAGGGT
    TCTCCCATCA TCACCTTCCC AGAGTTCTCA GGTTTTAATG AGGTTTCAGA TGAGGATTTT
    ATTAATGTTG TCACTTACCT GACCAGCATA CCTAGCATGG ATGCTGCTAG TATCGGCTTC
    ATCATCATCT TGGACCGAAG GAAGGATAAA TGGAACTCAG CAATCGCTTC TCTGGCACGC
    ATAGCGGGCT CCTTTCCAGG AAACCTACAG CTAGTTCTAG TTCTGAAACC TTCGCGCTTC
    CTCCAGAGGG CCATCGCTGA CGTCAGTATG AGGTTACACA GAGATGACTT CAAATTGAAG
    GTACCGATTG TTATGCTAAA TTCCCTGTCT GACTTGCATG GCTACATTGA TAAAGGCCAG
    TTGACCTGTG ACCTTGGGGG AACCCTGGAT TATTGCCACA GTCAATGGAT TCATCATCGC
    ACAGCCATTG AGAACTTTGC GCTGACAGTG AAGAGCACAG CACAGATGCT GCAGCAGTTC
    GGAACAGACC TGGCAGAGAC TGAACTTCCC AATGACGTGC AGTGCACCAA AGATTTGCTC
    ATCACACACA CTGAAAAACA CGATAAGCTC AAGGATGAAC TAAAATTGGC TGTAAAACAA
    GGGAATACTC TGCTGTCCTG TATTAAAGAC CAGGCCACCA AAGCCGAGAG CCACATTTTG
    AACCCAGACG AGGTTGAGAA CCAAACAACC GTTGAGAGGT TGTTAGCGCA GCTTGATGAG
    ACGGAGAATG CATTTGAACA ATTCTGGTCA AGACATCACC TCAAGTTAGA GCAGTGCCTA
    CAGTTACGAC ACTTTGAGCA GGACTTCAAA GAGGTGAAAG TGTCACTGGA CGGCCTCTCT
    GAAGCTTTGC TGGGACTGTC AGATACCGGG GACTGCGTGG CTCGTGTGGA ACAGCTATTA
    GGAGAACTGA AAACCTTGGA GGATAAAGCT CAGGAGTGTT TGGAGAAAGC TCAGCTTCAA
    GCTCAGCATG GGGATCAGCT CATTCAGAGT AATCACTACG CCATCGACTC CATCCGCCCC
    AAGTGTGTGG AACTGCGTCG GATCTGTGAC GACTTCAGCA ACGAGGCCAA AAAGAAGCGT
    GACGTTCTGA CCAAATCACT TGAAATCCTC AAACGAATTG ACGAGGTGAA CCAGTGGTGT
    GAAAGCGGAG TGTATCTGCT GGCCTCTCAG GCTGTAGACA GATGTCAAAC ACAGGAAGGT
    GCAGAAGCAG CACTGCAGGA CATCGAGAGG TACATGAAAA CAGCCAAAGA GCAGCAGCTC
    AGTCACCTCA AAGAACTCAA CAACCAGTAT GAGGTCGTCC TCTCAGAGCT GCTCAAGAGC
    AAAGTTCAAA CTGCCTTGAA AACGCTGAAC GATGTCCAAG AGATGTTTGA GAAAAGACAT
    GCAAGTCTGA AAAAGCTTTC CACGAAGCAA ACAAGGCCTG TGCAACCTGT TGCACCCCGA
    CCAGAATCTT CACCAAAACG CCCCTCACCT AAAATCCCAC ATCCCACTCC TCCAACATCA
    CACCGCAAAA CTACAGATAA CTCCAGCACC AATAGACAGC CCAGTGATGC AGAACTGGCA
    AAAAGAAAAA ACATTCGCAA AGCCAAAGGT GGCATTAAGA TTGAGGTCAT GCATGAAGGA
    GGTCAAGGAG GTTCAAGTCA TATTTTAATG AACAATGAAA CTGAAGAGTC GCTTTCAAAC
    AGACGCAGTC ACATTATGAA TGAACTAATT GAGACGGAAA GGCTGTATGT GGAAGAGCTG
    CAGAGCATCA TAGAGGGCTA TGCCGCGGCA CTCGATGACC CAGAACTCAT CTATCTGATC
    CCACCATCCC TGGAAAACAA GAAGGAAGTT CTCTTTGGAA ATCTGCCAGA AATTTACCAA
    TTCCACCAGA AGATATTTCT CAAAGAGCTG GAAAATTCTG CCGAAAAGCC GGAGTTGGTG
    GGAACATGTT TTTTGAAGCG GAAAGAAGAG TTGCAGATTT ATGAGAAATA TTGTCAGAAT
    AAGCCACGCT CTGAAGCTTT GTGGCGGCAA TGCGGGGACA GTATGTTCTT TCAGGAGTGT
    CAGAAAAGGT TGGACCACAA GCTTTCATTA GACGCGTATC TGCTAAAGCC TGTGCAGAGA
    ATCACCAAAT ACCAGCTTAT GTTAAAGGAG ATGCTGAAAT GCAGTAAGAA CCCAGAAGGC
    ACCGCTGAGC TGGAGGAAGC TCAGGCCACA GTGCTGGACA TCATCAAATC GGTCAACGAC
    TCCATGCATC AGATCGCCAT CACTGGTTTT GAGGGCAGTT TGAATGATCT GGGGAAACTG
    CTTATGCAGG GGTCGTTTAA CGTCTGGACA GACCATAAGA AAGGCCATAG TAAGGTGAAG
    GACTTGGCAC GCTTCAAACC CATGCAGAGA CATCTCTTCC TCTACCACAA AATGATATTG
    TTCTGCAAGA AACGTGAGGA GATGACTGAT GGCCATGAAA AATCCCCTTC CTACAGTTTC
    AAGCACTCAT TAAAGATGGG CTCAGTGGGA ATCACAGAAA ATGTAAAGGG AGACATCAAG
    AAGTTTGAAA TCTGGTACAA TGGCAGAGAA GAAGTTTACA TCATTCAGGC CCCGTCCATG
    GACGTGAAGA ACACGTGGGT ATCAGAAATC CGCAAAGTCT TAACAAGCCA GCTGGAAGCC
    TGCCGAGAAG CCAGTCAGCT CCATCAGAAG ATTACAGAGA ACATCTACCA TGCCCCCATG
    AGAAATATGC GTAAAACGGC CCTCAGACAG TCCGACTCTT CCAGTCCTGA AACAGGCTAC
    AGACGCAGTG ACTCGGGTCC AAATATGCGT CAGAAACGAG CTGACACTTC ATCCAGCCTC
    GGCTCAGAGC GAGCGGCTCT TCCTAGAAAG CGTTTCACCT TACAGGGCCT GTCAAACCGC
    AGATCTCTGC CCGCAGAACC CGCACCTAAG GAGACAGAAG TCACTCGCCG CTTTTCTTTA
    GCCTCAACTG TCGGCTCTGC CACTGCTCCA CGCACTAAAG CTCCTCTCTC CTCTAGCCTT
    AAGAGTAAGA GGCATCAGAT CAAGAGCGAC CCTACACCAT TTGGCTATGA AGGTGCTACC
    AAGACACGCG GTTTTAAAAA AGAAACCATG CGAGATTCCC TTCCAGCCGA GAGAAAAAGT
    AAAGGTAGCG GCGGTTCGAA GCCCGCTGTG CCGAGGTCTG CGTCTCAGCC AGGATGGAGT
    CAACGGCGTC TGCCTTCAAT GGACGCTGAG GATTTCGAGA CCATTCCGTC TAGTGCTGAA
    GAGCTGTCTA TTTCCTCTGA TGGAGAGGAT GAAGGACACA ACAAAAACGG GGATAATGAC
    CGCTATCAGG TGCAGTTGAC TTACGAGTCT TGCACTGCTC AGGATCTTTC TGTAGAAGCT
    GGAGATTTTG TACAATTCCT GGAAGAGTCA GAGAACGGCC TCTGGCTAGT CAAGAACCTT
    AGAACTCAGA AGACAGGACT GGTGCCGCCC ACTGTCCTGC AGTCCACAGC AGAGGAACTA
    GACCTTTACA GTAACTCCGA TATTTTCAGT GACCTCTGCA CCGCCGCACT CACCGATACG
    GAGGACGATA TCGGTGGGAC AAATCCTGCT CAGGACGGCA GATTTACATG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48789
    Clone ID Related Accession (Same CDS sequence) XM_009305855.2 , XM_009305855.3
    Accession Version XM_009305855.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATCAGGCC GTTGCTGGCT GTCGACATCA TTGAACAACT TCACAGGCAG 
    TTTGCTCTTC TTTCAGGAGG AAGAGGACAA GAGGGTTCTC CCATCATCAC CTTCCCAGAG
    TTCTCAGGTT TTAATGAGGT TTCAGATGAG GATTTTATTA ATGTTGTCAC TTACCTGACC
    AGCATACCTA GCATGGATGC TGCTAGTATC GGCTTCATCA TCATCTTGGA CCGAAGGAAG
    GATAAATGGA ACTCAGCAAT CGCTTCTCTG GCACGCATAG CGGGCTCCTT TCCAGGAAAC
    CTACAGCTAG TTCTAGTTCT GAAACCTTCG CGCTTCCTCC AGAGGGCCAT CGCTGACGTC
    AGTATGAGGT TACACAGAGA TGACTTCAAA TTGAAGGTAC CGATTGTTAT GCTAAATTCC
    CTGTCTGACT TGCATGGCTA CATTGATAAA GGCCAGTTGA CCTGTGACCT TGGGGGAACC
    CTGGATTATT GCCACAGTCA ATGGATTCAT CATCGCACAG CCATTGAGAA CTTTGCGCTG
    ACAGTGAAGA GCACAGCACA GATGCTGCAG CAGTTCGGAA CAGACCTGGC AGAGACTGAA
    CTTCCCAATG ACGTGCAGTG CACCAAAGAT TTGCTCATCA CACACACTGA AAAACACGAT
    AAGCTCAAGG ATGAACTAAA ATTGGCTGTA AAACAAGGGA ATACTCTGCT GTCCTGTATT
    AAAGACCAGG CCACCAAAGC CGAGAGCCAC ATTTTGAACC CAGACGAGGT TGAGAACCAA
    ACAACCGTTG AGAGGTTGTT AGCGCAGCTT GATGAGACGG AGAATGCATT TGAACAATTC
    TGGTCAAGAC ATCACCTCAA GTTAGAGCAG TGCCTACAGT TACGACACTT TGAGCAGGAC
    TTCAAAGAGG TGAAAGTGTC ACTGGACGGC CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT
    ACCGGGGACT GCGTGGCTCG TGTGGAACAG CTATTAGGAG AACTGAAAAC CTTGGAGGAT
    AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG CTTCAAGCTC AGCATGGGGA TCAGCTCATT
    CAGAGTAATC ACTACGCCAT CGACTCCATC CGCCCCAAGT GTGTGGAACT GCGTCGGATC
    TGTGACGACT TCAGCAACGA GGCCAAAAAG AAGCGTGACG TTCTGACCAA ATCACTTGAA
    ATCCTCAAAC GAATTGACGA GGTGAACCAG TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC
    TCTCAGGCTG TAGACAGATG TCAAACACAG GAAGGTGCAG AAGCAGCACT GCAGGACATC
    GAGAGGTACA TGAAAACAGC CAAAGAGCAG CAGCTCAGTC ACCTCAAAGA ACTCAACAAC
    CAGTATGAGG TCGTCCTCTC AGAGCTGCTC AAGAGCAAAG TTCAAACTGC CTTGAAAACG
    CTGAACGATG TCCAAGAGAT GTTTGAGAAA AGACATGCAA GTCTGAAAAA GCTTTCCACG
    AAGCAAACAA GGCCTGTGCA ACCTGTTGCA CCCCGACCAG AATCTTCACC AAAACGCCCC
    TCACCTAAAA TCCCACATCC CACTCCTCCA ACATCACACC GCAAAACTAC AGATAACTCC
    AGCACCAATA GACAGCCCAG TGATGCAGAA CTGGCAAAAA GAAAAAACAT TCGCAAAGCC
    AAAGGTGGCA TTAAGATTGA GGTCATGCAT GAAGGAGGTC AAGGAGGTTC AAGTCATATT
    TTAATGAACA ATGAAACTGA AGAGTCGCTT TCAAACAGAC GCAGTCACAT TATGAATGAA
    CTAATTGAGA CGGAAAGGCT GTATGTGGAA GAGCTGCAGA GCATCATAGA GGGCTATGCC
    GCGGCACTCG ATGACCCAGA ACTCATCTAT CTGATCCCAC CATCCCTGGA AAACAAGAAG
    GAAGTTCTCT TTGGAAATCT GCCAGAAATT TACCAATTCC ACCAGAAGAT ATTTCTCAAA
    GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG TTGGTGGGAA CATGTTTTTT GAAGCGGAAA
    GAAGAGTTGC AGATTTATGA GAAATATTGT CAGAATAAGC CACGCTCTGA AGCTTTGTGG
    CGGCAATGCG GGGACAGTAT GTTCTTTCAG GAGTGTCAGA AAAGGTTGGA CCACAAGCTT
    TCATTAGACG CGTATCTGCT AAAGCCTGTG CAGAGAATCA CCAAATACCA GCTTATGTTA
    AAGGAGATGC TGAAATGCAG TAAGAACCCA GAAGGCACCG CTGAGCTGGA GGAAGCTCAG
    GCCACAGTGC TGGACATCAT CAAATCGGTC AACGACTCCA TGCATCAGAT CGCCATCACT
    GGTTTTGAGG GCAGTTTGAA TGATCTGGGG AAACTGCTTA TGCAGGGGTC GTTTAACGTC
    TGGACAGACC ATAAGAAAGG CCATAGTAAG GTGAAGGACT TGGCACGCTT CAAACCCATG
    CAGAGACATC TCTTCCTCTA CCACAAAATG ATATTGTTCT GCAAGAAACG TGAGGAGATG
    ACTGATGGCC ATGAAAAATC CCCTTCCTAC AGTTTCAAGC ACTCATTAAA GATGGGCTCA
    GTGGGAATCA CAGAAAATGT AAAGGGAGAC ATCAAGAAGT TTGAAATCTG GTACAATGGC
    AGAGAAGAAG TTTACATCAT TCAGGCCCCG TCCATGGACG TGAAGAACAC GTGGGTATCA
    GAAATCCGCA AAGTCTTAAC AAGCCAGCTG GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT
    CAGAAGATTA CAGAGAACAT CTACCATGCC CCCATGAGAA ATATGCGTAA AACGGCCCTC
    AGACAGTCCG ACTCTTCCAG TCCTGAAACA GGCTACAGAC GCAGTGACTC GGGTCCAAAT
    ATGCGTCAGA AACGAGCTGA CACTTCATCC AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT
    AGAAAGCGTT TCACCTTACA GGGCCTGTCA AACCGCAGAT CTCTGCCCGC AGAACCCGCA
    CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT TCTTTAGCCT CAACTGTCGG CTCTGCCACT
    GCTCCACGCA CTAAAGCTCC TCTCTCCTCT AGCCTTAAGA GTAAGAGGCA TCAGATCAAG
    AGCGACCCTA CACCATTTGG CTATGAAGGT GCTACCAAGA CACGCGGTTT TAAAAAAGAA
    ACCATGCGAG ATTCCCTTCC AGCCGAGAGA AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC
    GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA TGGAGTCAAC GGCGTCTGCC TTCAATGGAC
    GCTGAGGATT TCGAGACCAT TCCGTCTAGT GCTGAAGAGC TGTCTATTTC CTCTGATGGA
    GAGGATGAAG GACACAACAA AAACGGGGAT AATGACCGCT ATCAGGTGCA GTTGACTTAC
    GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA GAAGCTGGAG ATTTTGTACA ATTCCTGGAA
    GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG AACCTTAGAA CTCAGAAGAC AGGACTGGTG
    CCGCCCACTG TCCTGCAGTC CACAGCAGAG GAACTAGACC TTTACAGTAA CTCCGATATT
    TTCAGTGACC TCTGCACCGC CGCACTCACC GATACGGAGG ACGATATCGG TGGGACAAAT
    CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304130.1
    CDS142..3831
    Translation

    Target ORF information:

    RefSeq Version XM_009305855.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305855.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATCAGGCC GTTGCTGGCT GTCGACATCA TTGAACAACT TCACAGGCAG 
    TTTGCTCTTC TTTCAGGAGG AAGAGGACAA GAGGGTTCTC CCATCATCAC CTTCCCAGAG
    TTCTCAGGTT TTAATGAGGT TTCAGATGAG GATTTTATTA ATGTTGTCAC TTACCTGACC
    AGCATACCTA GCATGGATGC TGCTAGTATC GGCTTCATCA TCATCTTGGA CCGAAGGAAG
    GATAAATGGA ACTCAGCAAT CGCTTCTCTG GCACGCATAG CGGGCTCCTT TCCAGGAAAC
    CTACAGCTAG TTCTAGTTCT GAAACCTTCG CGCTTCCTCC AGAGGGCCAT CGCTGACGTC
    AGTATGAGGT TACACAGAGA TGACTTCAAA TTGAAGGTAC CGATTGTTAT GCTAAATTCC
    CTGTCTGACT TGCATGGCTA CATTGATAAA GGCCAGTTGA CCTGTGACCT TGGGGGAACC
    CTGGATTATT GCCACAGTCA ATGGATTCAT CATCGCACAG CCATTGAGAA CTTTGCGCTG
    ACAGTGAAGA GCACAGCACA GATGCTGCAG CAGTTCGGAA CAGACCTGGC AGAGACTGAA
    CTTCCCAATG ACGTGCAGTG CACCAAAGAT TTGCTCATCA CACACACTGA AAAACACGAT
    AAGCTCAAGG ATGAACTAAA ATTGGCTGTA AAACAAGGGA ATACTCTGCT GTCCTGTATT
    AAAGACCAGG CCACCAAAGC CGAGAGCCAC ATTTTGAACC CAGACGAGGT TGAGAACCAA
    ACAACCGTTG AGAGGTTGTT AGCGCAGCTT GATGAGACGG AGAATGCATT TGAACAATTC
    TGGTCAAGAC ATCACCTCAA GTTAGAGCAG TGCCTACAGT TACGACACTT TGAGCAGGAC
    TTCAAAGAGG TGAAAGTGTC ACTGGACGGC CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT
    ACCGGGGACT GCGTGGCTCG TGTGGAACAG CTATTAGGAG AACTGAAAAC CTTGGAGGAT
    AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG CTTCAAGCTC AGCATGGGGA TCAGCTCATT
    CAGAGTAATC ACTACGCCAT CGACTCCATC CGCCCCAAGT GTGTGGAACT GCGTCGGATC
    TGTGACGACT TCAGCAACGA GGCCAAAAAG AAGCGTGACG TTCTGACCAA ATCACTTGAA
    ATCCTCAAAC GAATTGACGA GGTGAACCAG TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC
    TCTCAGGCTG TAGACAGATG TCAAACACAG GAAGGTGCAG AAGCAGCACT GCAGGACATC
    GAGAGGTACA TGAAAACAGC CAAAGAGCAG CAGCTCAGTC ACCTCAAAGA ACTCAACAAC
    CAGTATGAGG TCGTCCTCTC AGAGCTGCTC AAGAGCAAAG TTCAAACTGC CTTGAAAACG
    CTGAACGATG TCCAAGAGAT GTTTGAGAAA AGACATGCAA GTCTGAAAAA GCTTTCCACG
    AAGCAAACAA GGCCTGTGCA ACCTGTTGCA CCCCGACCAG AATCTTCACC AAAACGCCCC
    TCACCTAAAA TCCCACATCC CACTCCTCCA ACATCACACC GCAAAACTAC AGATAACTCC
    AGCACCAATA GACAGCCCAG TGATGCAGAA CTGGCAAAAA GAAAAAACAT TCGCAAAGCC
    AAAGGTGGCA TTAAGATTGA GGTCATGCAT GAAGGAGGTC AAGGAGGTTC AAGTCATATT
    TTAATGAACA ATGAAACTGA AGAGTCGCTT TCAAACAGAC GCAGTCACAT TATGAATGAA
    CTAATTGAGA CGGAAAGGCT GTATGTGGAA GAGCTGCAGA GCATCATAGA GGGCTATGCC
    GCGGCACTCG ATGACCCAGA ACTCATCTAT CTGATCCCAC CATCCCTGGA AAACAAGAAG
    GAAGTTCTCT TTGGAAATCT GCCAGAAATT TACCAATTCC ACCAGAAGAT ATTTCTCAAA
    GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG TTGGTGGGAA CATGTTTTTT GAAGCGGAAA
    GAAGAGTTGC AGATTTATGA GAAATATTGT CAGAATAAGC CACGCTCTGA AGCTTTGTGG
    CGGCAATGCG GGGACAGTAT GTTCTTTCAG GAGTGTCAGA AAAGGTTGGA CCACAAGCTT
    TCATTAGACG CGTATCTGCT AAAGCCTGTG CAGAGAATCA CCAAATACCA GCTTATGTTA
    AAGGAGATGC TGAAATGCAG TAAGAACCCA GAAGGCACCG CTGAGCTGGA GGAAGCTCAG
    GCCACAGTGC TGGACATCAT CAAATCGGTC AACGACTCCA TGCATCAGAT CGCCATCACT
    GGTTTTGAGG GCAGTTTGAA TGATCTGGGG AAACTGCTTA TGCAGGGGTC GTTTAACGTC
    TGGACAGACC ATAAGAAAGG CCATAGTAAG GTGAAGGACT TGGCACGCTT CAAACCCATG
    CAGAGACATC TCTTCCTCTA CCACAAAATG ATATTGTTCT GCAAGAAACG TGAGGAGATG
    ACTGATGGCC ATGAAAAATC CCCTTCCTAC AGTTTCAAGC ACTCATTAAA GATGGGCTCA
    GTGGGAATCA CAGAAAATGT AAAGGGAGAC ATCAAGAAGT TTGAAATCTG GTACAATGGC
    AGAGAAGAAG TTTACATCAT TCAGGCCCCG TCCATGGACG TGAAGAACAC GTGGGTATCA
    GAAATCCGCA AAGTCTTAAC AAGCCAGCTG GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT
    CAGAAGATTA CAGAGAACAT CTACCATGCC CCCATGAGAA ATATGCGTAA AACGGCCCTC
    AGACAGTCCG ACTCTTCCAG TCCTGAAACA GGCTACAGAC GCAGTGACTC GGGTCCAAAT
    ATGCGTCAGA AACGAGCTGA CACTTCATCC AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT
    AGAAAGCGTT TCACCTTACA GGGCCTGTCA AACCGCAGAT CTCTGCCCGC AGAACCCGCA
    CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT TCTTTAGCCT CAACTGTCGG CTCTGCCACT
    GCTCCACGCA CTAAAGCTCC TCTCTCCTCT AGCCTTAAGA GTAAGAGGCA TCAGATCAAG
    AGCGACCCTA CACCATTTGG CTATGAAGGT GCTACCAAGA CACGCGGTTT TAAAAAAGAA
    ACCATGCGAG ATTCCCTTCC AGCCGAGAGA AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC
    GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA TGGAGTCAAC GGCGTCTGCC TTCAATGGAC
    GCTGAGGATT TCGAGACCAT TCCGTCTAGT GCTGAAGAGC TGTCTATTTC CTCTGATGGA
    GAGGATGAAG GACACAACAA AAACGGGGAT AATGACCGCT ATCAGGTGCA GTTGACTTAC
    GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA GAAGCTGGAG ATTTTGTACA ATTCCTGGAA
    GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG AACCTTAGAA CTCAGAAGAC AGGACTGGTG
    CCGCCCACTG TCCTGCAGTC CACAGCAGAG GAACTAGACC TTTACAGTAA CTCCGATATT
    TTCAGTGACC TCTGCACCGC CGCACTCACC GATACGGAGG ACGATATCGG TGGGACAAAT
    CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa48789
    Clone ID Related Accession (Same CDS sequence) XM_009305855.2 , XM_009305855.3
    Accession Version XM_009305855.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product guanine nucleotide exchange factor DBS isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATCAGGCC GTTGCTGGCT GTCGACATCA TTGAACAACT TCACAGGCAG 
    TTTGCTCTTC TTTCAGGAGG AAGAGGACAA GAGGGTTCTC CCATCATCAC CTTCCCAGAG
    TTCTCAGGTT TTAATGAGGT TTCAGATGAG GATTTTATTA ATGTTGTCAC TTACCTGACC
    AGCATACCTA GCATGGATGC TGCTAGTATC GGCTTCATCA TCATCTTGGA CCGAAGGAAG
    GATAAATGGA ACTCAGCAAT CGCTTCTCTG GCACGCATAG CGGGCTCCTT TCCAGGAAAC
    CTACAGCTAG TTCTAGTTCT GAAACCTTCG CGCTTCCTCC AGAGGGCCAT CGCTGACGTC
    AGTATGAGGT TACACAGAGA TGACTTCAAA TTGAAGGTAC CGATTGTTAT GCTAAATTCC
    CTGTCTGACT TGCATGGCTA CATTGATAAA GGCCAGTTGA CCTGTGACCT TGGGGGAACC
    CTGGATTATT GCCACAGTCA ATGGATTCAT CATCGCACAG CCATTGAGAA CTTTGCGCTG
    ACAGTGAAGA GCACAGCACA GATGCTGCAG CAGTTCGGAA CAGACCTGGC AGAGACTGAA
    CTTCCCAATG ACGTGCAGTG CACCAAAGAT TTGCTCATCA CACACACTGA AAAACACGAT
    AAGCTCAAGG ATGAACTAAA ATTGGCTGTA AAACAAGGGA ATACTCTGCT GTCCTGTATT
    AAAGACCAGG CCACCAAAGC CGAGAGCCAC ATTTTGAACC CAGACGAGGT TGAGAACCAA
    ACAACCGTTG AGAGGTTGTT AGCGCAGCTT GATGAGACGG AGAATGCATT TGAACAATTC
    TGGTCAAGAC ATCACCTCAA GTTAGAGCAG TGCCTACAGT TACGACACTT TGAGCAGGAC
    TTCAAAGAGG TGAAAGTGTC ACTGGACGGC CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT
    ACCGGGGACT GCGTGGCTCG TGTGGAACAG CTATTAGGAG AACTGAAAAC CTTGGAGGAT
    AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG CTTCAAGCTC AGCATGGGGA TCAGCTCATT
    CAGAGTAATC ACTACGCCAT CGACTCCATC CGCCCCAAGT GTGTGGAACT GCGTCGGATC
    TGTGACGACT TCAGCAACGA GGCCAAAAAG AAGCGTGACG TTCTGACCAA ATCACTTGAA
    ATCCTCAAAC GAATTGACGA GGTGAACCAG TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC
    TCTCAGGCTG TAGACAGATG TCAAACACAG GAAGGTGCAG AAGCAGCACT GCAGGACATC
    GAGAGGTACA TGAAAACAGC CAAAGAGCAG CAGCTCAGTC ACCTCAAAGA ACTCAACAAC
    CAGTATGAGG TCGTCCTCTC AGAGCTGCTC AAGAGCAAAG TTCAAACTGC CTTGAAAACG
    CTGAACGATG TCCAAGAGAT GTTTGAGAAA AGACATGCAA GTCTGAAAAA GCTTTCCACG
    AAGCAAACAA GGCCTGTGCA ACCTGTTGCA CCCCGACCAG AATCTTCACC AAAACGCCCC
    TCACCTAAAA TCCCACATCC CACTCCTCCA ACATCACACC GCAAAACTAC AGATAACTCC
    AGCACCAATA GACAGCCCAG TGATGCAGAA CTGGCAAAAA GAAAAAACAT TCGCAAAGCC
    AAAGGTGGCA TTAAGATTGA GGTCATGCAT GAAGGAGGTC AAGGAGGTTC AAGTCATATT
    TTAATGAACA ATGAAACTGA AGAGTCGCTT TCAAACAGAC GCAGTCACAT TATGAATGAA
    CTAATTGAGA CGGAAAGGCT GTATGTGGAA GAGCTGCAGA GCATCATAGA GGGCTATGCC
    GCGGCACTCG ATGACCCAGA ACTCATCTAT CTGATCCCAC CATCCCTGGA AAACAAGAAG
    GAAGTTCTCT TTGGAAATCT GCCAGAAATT TACCAATTCC ACCAGAAGAT ATTTCTCAAA
    GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG TTGGTGGGAA CATGTTTTTT GAAGCGGAAA
    GAAGAGTTGC AGATTTATGA GAAATATTGT CAGAATAAGC CACGCTCTGA AGCTTTGTGG
    CGGCAATGCG GGGACAGTAT GTTCTTTCAG GAGTGTCAGA AAAGGTTGGA CCACAAGCTT
    TCATTAGACG CGTATCTGCT AAAGCCTGTG CAGAGAATCA CCAAATACCA GCTTATGTTA
    AAGGAGATGC TGAAATGCAG TAAGAACCCA GAAGGCACCG CTGAGCTGGA GGAAGCTCAG
    GCCACAGTGC TGGACATCAT CAAATCGGTC AACGACTCCA TGCATCAGAT CGCCATCACT
    GGTTTTGAGG GCAGTTTGAA TGATCTGGGG AAACTGCTTA TGCAGGGGTC GTTTAACGTC
    TGGACAGACC ATAAGAAAGG CCATAGTAAG GTGAAGGACT TGGCACGCTT CAAACCCATG
    CAGAGACATC TCTTCCTCTA CCACAAAATG ATATTGTTCT GCAAGAAACG TGAGGAGATG
    ACTGATGGCC ATGAAAAATC CCCTTCCTAC AGTTTCAAGC ACTCATTAAA GATGGGCTCA
    GTGGGAATCA CAGAAAATGT AAAGGGAGAC ATCAAGAAGT TTGAAATCTG GTACAATGGC
    AGAGAAGAAG TTTACATCAT TCAGGCCCCG TCCATGGACG TGAAGAACAC GTGGGTATCA
    GAAATCCGCA AAGTCTTAAC AAGCCAGCTG GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT
    CAGAAGATTA CAGAGAACAT CTACCATGCC CCCATGAGAA ATATGCGTAA AACGGCCCTC
    AGACAGTCCG ACTCTTCCAG TCCTGAAACA GGCTACAGAC GCAGTGACTC GGGTCCAAAT
    ATGCGTCAGA AACGAGCTGA CACTTCATCC AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT
    AGAAAGCGTT TCACCTTACA GGGCCTGTCA AACCGCAGAT CTCTGCCCGC AGAACCCGCA
    CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT TCTTTAGCCT CAACTGTCGG CTCTGCCACT
    GCTCCACGCA CTAAAGCTCC TCTCTCCTCT AGCCTTAAGA GTAAGAGGCA TCAGATCAAG
    AGCGACCCTA CACCATTTGG CTATGAAGGT GCTACCAAGA CACGCGGTTT TAAAAAAGAA
    ACCATGCGAG ATTCCCTTCC AGCCGAGAGA AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC
    GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA TGGAGTCAAC GGCGTCTGCC TTCAATGGAC
    GCTGAGGATT TCGAGACCAT TCCGTCTAGT GCTGAAGAGC TGTCTATTTC CTCTGATGGA
    GAGGATGAAG GACACAACAA AAACGGGGAT AATGACCGCT ATCAGGTGCA GTTGACTTAC
    GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA GAAGCTGGAG ATTTTGTACA ATTCCTGGAA
    GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG AACCTTAGAA CTCAGAAGAC AGGACTGGTG
    CCGCCCACTG TCCTGCAGTC CACAGCAGAG GAACTAGACC TTTACAGTAA CTCCGATATT
    TTCAGTGACC TCTGCACCGC CGCACTCACC GATACGGAGG ACGATATCGG TGGGACAAAT
    CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009304130.1
    CDS181..3870
    Translation

    Target ORF information:

    RefSeq Version XM_009305855.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio MCF.2 cell line derived transforming sequence-like 2 (mcf2l2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009305855.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCAGCAGG AGATCAGGCC GTTGCTGGCT GTCGACATCA TTGAACAACT TCACAGGCAG 
    TTTGCTCTTC TTTCAGGAGG AAGAGGACAA GAGGGTTCTC CCATCATCAC CTTCCCAGAG
    TTCTCAGGTT TTAATGAGGT TTCAGATGAG GATTTTATTA ATGTTGTCAC TTACCTGACC
    AGCATACCTA GCATGGATGC TGCTAGTATC GGCTTCATCA TCATCTTGGA CCGAAGGAAG
    GATAAATGGA ACTCAGCAAT CGCTTCTCTG GCACGCATAG CGGGCTCCTT TCCAGGAAAC
    CTACAGCTAG TTCTAGTTCT GAAACCTTCG CGCTTCCTCC AGAGGGCCAT CGCTGACGTC
    AGTATGAGGT TACACAGAGA TGACTTCAAA TTGAAGGTAC CGATTGTTAT GCTAAATTCC
    CTGTCTGACT TGCATGGCTA CATTGATAAA GGCCAGTTGA CCTGTGACCT TGGGGGAACC
    CTGGATTATT GCCACAGTCA ATGGATTCAT CATCGCACAG CCATTGAGAA CTTTGCGCTG
    ACAGTGAAGA GCACAGCACA GATGCTGCAG CAGTTCGGAA CAGACCTGGC AGAGACTGAA
    CTTCCCAATG ACGTGCAGTG CACCAAAGAT TTGCTCATCA CACACACTGA AAAACACGAT
    AAGCTCAAGG ATGAACTAAA ATTGGCTGTA AAACAAGGGA ATACTCTGCT GTCCTGTATT
    AAAGACCAGG CCACCAAAGC CGAGAGCCAC ATTTTGAACC CAGACGAGGT TGAGAACCAA
    ACAACCGTTG AGAGGTTGTT AGCGCAGCTT GATGAGACGG AGAATGCATT TGAACAATTC
    TGGTCAAGAC ATCACCTCAA GTTAGAGCAG TGCCTACAGT TACGACACTT TGAGCAGGAC
    TTCAAAGAGG TGAAAGTGTC ACTGGACGGC CTCTCTGAAG CTTTGCTGGG ACTGTCAGAT
    ACCGGGGACT GCGTGGCTCG TGTGGAACAG CTATTAGGAG AACTGAAAAC CTTGGAGGAT
    AAAGCTCAGG AGTGTTTGGA GAAAGCTCAG CTTCAAGCTC AGCATGGGGA TCAGCTCATT
    CAGAGTAATC ACTACGCCAT CGACTCCATC CGCCCCAAGT GTGTGGAACT GCGTCGGATC
    TGTGACGACT TCAGCAACGA GGCCAAAAAG AAGCGTGACG TTCTGACCAA ATCACTTGAA
    ATCCTCAAAC GAATTGACGA GGTGAACCAG TGGTGTGAAA GCGGAGTGTA TCTGCTGGCC
    TCTCAGGCTG TAGACAGATG TCAAACACAG GAAGGTGCAG AAGCAGCACT GCAGGACATC
    GAGAGGTACA TGAAAACAGC CAAAGAGCAG CAGCTCAGTC ACCTCAAAGA ACTCAACAAC
    CAGTATGAGG TCGTCCTCTC AGAGCTGCTC AAGAGCAAAG TTCAAACTGC CTTGAAAACG
    CTGAACGATG TCCAAGAGAT GTTTGAGAAA AGACATGCAA GTCTGAAAAA GCTTTCCACG
    AAGCAAACAA GGCCTGTGCA ACCTGTTGCA CCCCGACCAG AATCTTCACC AAAACGCCCC
    TCACCTAAAA TCCCACATCC CACTCCTCCA ACATCACACC GCAAAACTAC AGATAACTCC
    AGCACCAATA GACAGCCCAG TGATGCAGAA CTGGCAAAAA GAAAAAACAT TCGCAAAGCC
    AAAGGTGGCA TTAAGATTGA GGTCATGCAT GAAGGAGGTC AAGGAGGTTC AAGTCATATT
    TTAATGAACA ATGAAACTGA AGAGTCGCTT TCAAACAGAC GCAGTCACAT TATGAATGAA
    CTAATTGAGA CGGAAAGGCT GTATGTGGAA GAGCTGCAGA GCATCATAGA GGGCTATGCC
    GCGGCACTCG ATGACCCAGA ACTCATCTAT CTGATCCCAC CATCCCTGGA AAACAAGAAG
    GAAGTTCTCT TTGGAAATCT GCCAGAAATT TACCAATTCC ACCAGAAGAT ATTTCTCAAA
    GAGCTGGAAA ATTCTGCCGA AAAGCCGGAG TTGGTGGGAA CATGTTTTTT GAAGCGGAAA
    GAAGAGTTGC AGATTTATGA GAAATATTGT CAGAATAAGC CACGCTCTGA AGCTTTGTGG
    CGGCAATGCG GGGACAGTAT GTTCTTTCAG GAGTGTCAGA AAAGGTTGGA CCACAAGCTT
    TCATTAGACG CGTATCTGCT AAAGCCTGTG CAGAGAATCA CCAAATACCA GCTTATGTTA
    AAGGAGATGC TGAAATGCAG TAAGAACCCA GAAGGCACCG CTGAGCTGGA GGAAGCTCAG
    GCCACAGTGC TGGACATCAT CAAATCGGTC AACGACTCCA TGCATCAGAT CGCCATCACT
    GGTTTTGAGG GCAGTTTGAA TGATCTGGGG AAACTGCTTA TGCAGGGGTC GTTTAACGTC
    TGGACAGACC ATAAGAAAGG CCATAGTAAG GTGAAGGACT TGGCACGCTT CAAACCCATG
    CAGAGACATC TCTTCCTCTA CCACAAAATG ATATTGTTCT GCAAGAAACG TGAGGAGATG
    ACTGATGGCC ATGAAAAATC CCCTTCCTAC AGTTTCAAGC ACTCATTAAA GATGGGCTCA
    GTGGGAATCA CAGAAAATGT AAAGGGAGAC ATCAAGAAGT TTGAAATCTG GTACAATGGC
    AGAGAAGAAG TTTACATCAT TCAGGCCCCG TCCATGGACG TGAAGAACAC GTGGGTATCA
    GAAATCCGCA AAGTCTTAAC AAGCCAGCTG GAAGCCTGCC GAGAAGCCAG TCAGCTCCAT
    CAGAAGATTA CAGAGAACAT CTACCATGCC CCCATGAGAA ATATGCGTAA AACGGCCCTC
    AGACAGTCCG ACTCTTCCAG TCCTGAAACA GGCTACAGAC GCAGTGACTC GGGTCCAAAT
    ATGCGTCAGA AACGAGCTGA CACTTCATCC AGCCTCGGCT CAGAGCGAGC GGCTCTTCCT
    AGAAAGCGTT TCACCTTACA GGGCCTGTCA AACCGCAGAT CTCTGCCCGC AGAACCCGCA
    CCTAAGGAGA CAGAAGTCAC TCGCCGCTTT TCTTTAGCCT CAACTGTCGG CTCTGCCACT
    GCTCCACGCA CTAAAGCTCC TCTCTCCTCT AGCCTTAAGA GTAAGAGGCA TCAGATCAAG
    AGCGACCCTA CACCATTTGG CTATGAAGGT GCTACCAAGA CACGCGGTTT TAAAAAAGAA
    ACCATGCGAG ATTCCCTTCC AGCCGAGAGA AAAAGTAAAG GTAGCGGCGG TTCGAAGCCC
    GCTGTGCCGA GGTCTGCGTC TCAGCCAGGA TGGAGTCAAC GGCGTCTGCC TTCAATGGAC
    GCTGAGGATT TCGAGACCAT TCCGTCTAGT GCTGAAGAGC TGTCTATTTC CTCTGATGGA
    GAGGATGAAG GACACAACAA AAACGGGGAT AATGACCGCT ATCAGGTGCA GTTGACTTAC
    GAGTCTTGCA CTGCTCAGGA TCTTTCTGTA GAAGCTGGAG ATTTTGTACA ATTCCTGGAA
    GAGTCAGAGA ACGGCCTCTG GCTAGTCAAG AACCTTAGAA CTCAGAAGAC AGGACTGGTG
    CCGCCCACTG TCCTGCAGTC CACAGCAGAG GAACTAGACC TTTACAGTAA CTCCGATATT
    TTCAGTGACC TCTGCACCGC CGCACTCACC GATACGGAGG ACGATATCGG TGGGACAAAT
    CCTGCTCAGG ACGGCAGATT TACATGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.