MIB1 cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIB1
Entrez Gene ID	421069
Full Name	mindbomb E3 ubiquitin protein ligase 1
General protein information	Preferred Names mindbomb E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase MIB1 mindbomb homolog 1 ubiquitin ligase mind bomb
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004939770.3	XP_004939827.2	E3 ubiquitin-protein ligase MIB1 isoform X3
XM_419157.5	XP_419157.3	E3 ubiquitin-protein ligase MIB1 isoform X1
XM_419157.6	XP_419157.4	E3 ubiquitin-protein ligase MIB1 isoform X1
XM_004939769.2	XP_004939826.1	E3 ubiquitin-protein ligase MIB1 isoform X2
XM_004939770.2	XP_004939827.1	E3 ubiquitin-protein ligase MIB1 isoform X3
XM_004939769.3	XP_004939826.2	E3 ubiquitin-protein ligase MIB1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MIB1	NP_065825.1
Mus musculus (house mouse)	Mib1	NP_659109.2
Gallus gallus (chicken)	MIB1	XP_419157.3
Danio rerio (zebrafish)	mib	NP_775393.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011932	XP_320601.4
Canis lupus familiaris (dog)	MIB1	XP_547643.4
Drosophila melanogaster (fruit fly)	mib1	NP_648826.2
Xenopus tropicalis (tropical clawed frog)	mib1	NP_001123407.1
Pan troglodytes (chimpanzee)	MIB1	XP_003315915.1
	MIB1	XP_001092086.1
Bos taurus (cattle)	MIB1	NP_001192959.1
Rattus norvegicus (Norway rat)	Mib1	NP_001100875.1

Gene Ontology

The following MIB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa146349	XM_004939769.3 Latest version!	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa146350	XM_004939770.3 Latest version!	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa146348	XM_419157.6 Latest version!	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa05129	XM_419157.5 Latest version!	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa05130	XM_004939769.2 Latest version!	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OGa05131	XM_004939770.2 Latest version!	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIB1 ( XM_004939769.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939826.2 Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa146349
Clone ID Related Accession (Same CDS sequence)	XM_004939769.3
Accession Version	XM_004939769.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase MIB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC 
AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC 
CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT 
GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT 
GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA 
AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC 
CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT 
GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA 
ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT 
GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT 
GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC 
AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT 
GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA 
ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT 
GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA 
GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA 
GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA 
GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC 
TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG 
GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC 
AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA 
CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG 
AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT 
GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC 
GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA 
GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT 
TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT 
GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT 
CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA 
CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT 
CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT 
ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG 
ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT 
ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC 
ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG 
GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA 
GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC 
CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG 
GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG 
GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT 
TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG 
ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT 
TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT 
CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGCAAGTGGT 
AACATTCCAG TTATGCAGAA AGACAAAGAC AACACTAACG TCAATGCAGA TGTACAGAAG 
CTGCAGCAGC AGTTACAGGA CATCAAGGAA CAGACTATGT GTCCTGTCTG TTTGGACCGT 
CTGAAGAATA TGATCTTTCT CTGTGGCCAT GGGACGTGTC AGCTCTGTGG AGACCGAATG 
AGTGAATGCC CTATATGCCG CAAGGCAATC GAACGAAGGA TTCTTTTGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939826.2
CDS	294..3287
Translation	MSSSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMINNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFIMCCGGKASGNIPVMQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_004939769.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939769.3

ORF Insert Sequence:

ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC 
AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC 
CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT 
GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT 
GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA 
AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC 
CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT 
GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA 
ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT 
GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT 
GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC 
AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT 
GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA 
ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT 
GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA 
GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA 
GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA 
GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC 
TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG 
GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC 
AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA 
CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG 
AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT 
GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC 
GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA 
GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT 
TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT 
GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT 
CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA 
CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT 
CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT 
ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG 
ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT 
ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC 
ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG 
GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA 
GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC 
CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG 
GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG 
GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT 
TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG 
ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT 
TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT 
CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGCAAGTGGT 
AACATTCCAG TTATGCAGAA AGACAAAGAC AACACTAACG TCAATGCAGA TGTACAGAAG 
CTGCAGCAGC AGTTACAGGA CATCAAGGAA CAGACTATGT GTCCTGTCTG TTTGGACCGT 
CTGAAGAATA TGATCTTTCT CTGTGGCCAT GGGACGTGTC AGCTCTGTGG AGACCGAATG 
AGTGAATGCC CTATATGCCG CAAGGCAATC GAACGAAGGA TTCTTTTGTA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB1 ( XM_004939770.3 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939827.2 Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa146350
Clone ID Related Accession (Same CDS sequence)	XM_004939770.3
Accession Version	XM_004939770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase MIB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC 
AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC 
CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT 
GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT 
GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA 
AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC 
CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT 
GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA 
ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT 
GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT 
GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC 
AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT 
GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA 
ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT 
GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA 
GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA 
GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA 
GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC 
TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG 
GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC 
AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA 
CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG 
AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT 
GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC 
GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA 
GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT 
TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT 
GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT 
CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA 
CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT 
CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT 
ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG 
ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT 
ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC 
ATGCAAGATG TGGGCAAGTT AATAATGGGA CTTGGCACTC AGGGAGCAGA GAAGAAGAGT 
GCAGCATCTA TTGCCTGCTT CCTGGCAGCC AACGGAGCCG ACCTCAGCAT TCGTAATAAG 
AAGGGTCAGT CTCCTCTTGA TCTCTGCCCT GACCCTAGCC TCTGCAAAGC ACTGGCAAAA 
TGCCACAAAG AAAAAGTCAG TGGTCAGGTT GGTTCCCGAA GTCCATCTAT GATCAACAAT 
GACTCTGAAA CCTTAGAAGA ATGCATGGTG TGTTCAGATA TGAAAAGAGA TACTCTGTTT 
GGCCCGTGTG GACATATTGC CACTTGTTCT CTGTGCTCAC CACGGGTGAA AAAATGCCTC 
ATCTGTAAAG AACAAGTTCA GTCGAGGACA AAGATTGAAG AATGCGTAGT GTGTTCGGAC 
AAAAAGGCGG CAGTCCTTTT CCAGCCTTGT GGTCATATGT GTGCTTGTGA GAATTGTGCA 
AGCTTGATGA AGAAATGTGT ACAGTGTCGG GCAGTAGTAG AACGAAGAGT GCCTTTCATA 
ATGTGCTGTG GAGGGAAAGG TACAGAAGAT ACCACTGATG ATATTTCAAG TGGTAACATT 
CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG 
CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG 
AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA 
TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939827.2
CDS	294..3281
Translation	MSSSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMINNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFIMCCGGKGTEDTTDDISSGNIPVMQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_004939770.3
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939770.3

ORF Insert Sequence:

ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC 
AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC 
CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT 
GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT 
GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA 
AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC 
CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT 
GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA 
ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT 
GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT 
GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC 
AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT 
GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA 
ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT 
GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA 
GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA 
GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA 
GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC 
TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG 
GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC 
AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA 
CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG 
AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT 
GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC 
GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA 
GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT 
TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT 
GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT 
CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA 
CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT 
CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT 
ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG 
ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT 
ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC 
ATGCAAGATG TGGGCAAGTT AATAATGGGA CTTGGCACTC AGGGAGCAGA GAAGAAGAGT 
GCAGCATCTA TTGCCTGCTT CCTGGCAGCC AACGGAGCCG ACCTCAGCAT TCGTAATAAG 
AAGGGTCAGT CTCCTCTTGA TCTCTGCCCT GACCCTAGCC TCTGCAAAGC ACTGGCAAAA 
TGCCACAAAG AAAAAGTCAG TGGTCAGGTT GGTTCCCGAA GTCCATCTAT GATCAACAAT 
GACTCTGAAA CCTTAGAAGA ATGCATGGTG TGTTCAGATA TGAAAAGAGA TACTCTGTTT 
GGCCCGTGTG GACATATTGC CACTTGTTCT CTGTGCTCAC CACGGGTGAA AAAATGCCTC 
ATCTGTAAAG AACAAGTTCA GTCGAGGACA AAGATTGAAG AATGCGTAGT GTGTTCGGAC 
AAAAAGGCGG CAGTCCTTTT CCAGCCTTGT GGTCATATGT GTGCTTGTGA GAATTGTGCA 
AGCTTGATGA AGAAATGTGT ACAGTGTCGG GCAGTAGTAG AACGAAGAGT GCCTTTCATA 
ATGTGCTGTG GAGGGAAAGG TACAGAAGAT ACCACTGATG ATATTTCAAG TGGTAACATT 
CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG 
CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG 
AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA 
TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB1 ( XM_419157.6 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419157.4 Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa146348
Clone ID Related Accession (Same CDS sequence)	XM_419157.6
Accession Version	XM_419157.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase MIB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_419157.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC 
AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC 
CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT 
GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT 
GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA 
AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC 
CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT 
GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA 
ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT 
GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT 
GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC 
AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT 
GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA 
ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT 
GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA 
GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA 
GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA 
GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC 
TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG 
GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC 
AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA 
CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG 
AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT 
GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC 
GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA 
GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT 
TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT 
GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT 
CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA 
CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT 
CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT 
ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG 
ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT 
ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC 
ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG 
GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA 
GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC 
CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG 
GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG 
GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT 
TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG 
ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT 
TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT 
CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGGTACAGAA 
GATACCACTG ATGATATTTC AAGTGGTAAC ATTCCAGTTA TGCAGAAAGA CAAAGACAAC 
ACTAACGTCA ATGCAGATGT ACAGAAGCTG CAGCAGCAGT TACAGGACAT CAAGGAACAG 
ACTATGTGTC CTGTCTGTTT GGACCGTCTG AAGAATATGA TCTTTCTCTG TGGCCATGGG 
ACGTGTCAGC TCTGTGGAGA CCGAATGAGT GAATGCCCTA TATGCCGCAA GGCAATCGAA 
CGAAGGATTC TTTTGTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419157.4
CDS	294..3314
Translation	MSSSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMINNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFIMCCGGKGTEDTTDDISSGNIPVMQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_419157.6
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419157.6

ORF Insert Sequence:

ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC 
AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC 
CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT 
GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT 
GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA 
AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC 
CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT 
GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA 
ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT 
GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT 
GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC 
AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT 
GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA 
ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT 
GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA 
GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA 
GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA 
GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC 
TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG 
GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG 
AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC 
AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA 
CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG 
AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT 
GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC 
GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA 
GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT 
TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT 
GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT 
CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA 
CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT 
CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT 
ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG 
ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT 
ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC 
ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG 
GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA 
GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC 
CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG 
GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG 
GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT 
TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG 
ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT 
TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT 
CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGGTACAGAA 
GATACCACTG ATGATATTTC AAGTGGTAAC ATTCCAGTTA TGCAGAAAGA CAAAGACAAC 
ACTAACGTCA ATGCAGATGT ACAGAAGCTG CAGCAGCAGT TACAGGACAT CAAGGAACAG 
ACTATGTGTC CTGTCTGTTT GGACCGTCTG AAGAATATGA TCTTTCTCTG TGGCCATGGG 
ACGTGTCAGC TCTGTGGAGA CCGAATGAGT GAATGCCCTA TATGCCGCAA GGCAATCGAA 
CGAAGGATTC TTTTGTATTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB1 ( XM_419157.5 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_419157.3 Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa05129
Clone ID Related Accession (Same CDS sequence)	XM_419157.5
Accession Version	XM_419157.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase MIB1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419157.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG 
CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG 
TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC 
ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC 
AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC 
GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC 
GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC 
TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG 
ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA 
TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT 
CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA 
TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC 
TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA 
GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA 
AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA 
GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT 
CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG 
TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT 
GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT 
AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT 
GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC 
TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG 
CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG 
GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA 
GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA 
TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA 
GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT 
GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG 
CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT 
CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT 
CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT 
CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA 
GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG 
TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT 
GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG 
TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC 
AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG 
AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT 
AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC 
CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA 
GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT 
GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC 
GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC 
CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT 
TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT 
TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG 
TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG 
ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT 
CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA 
GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGGTAC AGAAGATACC 
ACTGATGATA TTTCAAGTGG TAACATTCCA GTTATGCAGA AAGACAAAGA CAACACTAAC 
GTCAATGCAG ATGTACAGAA GCTGCAGCAG CAGTTACAGG ACATCAAGGA ACAGACTATG 
TGTCCTGTCT GTTTGGACCG TCTGAAGAAT ATGATCTTTC TCTGTGGCCA TGGGACGTGT 
CAGCTCTGTG GAGACCGAAT GAGTGAATGC CCTATATGCC GCAAGGCAAT CGAACGAAGG 
ATTCTTTTGT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_419157.3
CDS	3..3317
Translation	MRRRGGGGCRPPPVSLVPPPSRPQSPDVAAVAGRRRAARWREESGPQACARQPPRLPGVASGSPLSAQRRPAASRRVPGPIGVPVGPPRRPGPRGAGGMSSSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMINNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFIMCCGGKGTEDTTDDISSGNIPVMQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_419157.5
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_419157.5

ORF Insert Sequence:

ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG 
CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG 
TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC 
ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC 
AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC 
GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC 
GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC 
TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG 
ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA 
TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT 
CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA 
TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC 
TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA 
GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA 
AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA 
GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT 
CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG 
TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT 
GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT 
AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT 
GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC 
TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG 
CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG 
GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA 
GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA 
TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA 
GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT 
GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG 
CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT 
CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT 
CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT 
CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA 
GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG 
TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT 
GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG 
TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC 
AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG 
AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT 
AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC 
CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA 
GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT 
GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC 
GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC 
CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT 
TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT 
TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG 
TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG 
ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT 
CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA 
GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGGTAC AGAAGATACC 
ACTGATGATA TTTCAAGTGG TAACATTCCA GTTATGCAGA AAGACAAAGA CAACACTAAC 
GTCAATGCAG ATGTACAGAA GCTGCAGCAG CAGTTACAGG ACATCAAGGA ACAGACTATG 
TGTCCTGTCT GTTTGGACCG TCTGAAGAAT ATGATCTTTC TCTGTGGCCA TGGGACGTGT 
CAGCTCTGTG GAGACCGAAT GAGTGAATGC CCTATATGCC GCAAGGCAAT CGAACGAAGG 
ATTCTTTTGT ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB1 ( XM_004939769.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939826.1 Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.

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CloneID	OGa05130
Clone ID Related Accession (Same CDS sequence)	XM_004939769.2
Accession Version	XM_004939769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3288bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase MIB1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG 
CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG 
TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC 
ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC 
AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC 
GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC 
GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC 
TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG 
ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA 
TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT 
CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA 
TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC 
TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA 
GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA 
AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA 
GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT 
CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG 
TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT 
GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT 
AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT 
GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC 
TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG 
CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG 
GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA 
GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA 
TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA 
GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT 
GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG 
CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT 
CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT 
CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT 
CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA 
GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG 
TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT 
GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG 
TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC 
AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG 
AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT 
AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC 
CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA 
GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT 
GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC 
GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC 
CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT 
TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT 
TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG 
TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG 
ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT 
CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA 
GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGCAAG TGGTAACATT 
CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG 
CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG 
AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA 
TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939826.1
CDS	3..3290
Translation	MRRRGGGGCRPPPVSLVPPPSRPQSPDVAAVAGRRRAARWREESGPQACARQPPRLPGVASGSPLSAQRRPAASRRVPGPIGVPVGPPRRPGPRGAGGMSSSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKVDTAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMINNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFIMCCGGKASGNIPVMQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_004939769.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939769.2

ORF Insert Sequence:

ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG 
CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG 
TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC 
ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC 
AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC 
GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC 
GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC 
TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG 
ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA 
TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT 
CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA 
TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC 
TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA 
GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA 
AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA 
GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT 
CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG 
TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT 
GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT 
AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT 
GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC 
TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG 
CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG 
GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA 
GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA 
TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA 
GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT 
GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG 
CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT 
CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT 
CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT 
CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA 
GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG 
TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT 
GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG 
TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC 
AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG 
AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT 
AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC 
CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA 
GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT 
GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC 
GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC 
CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT 
TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT 
TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG 
TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG 
ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT 
CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA 
GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGCAAG TGGTAACATT 
CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG 
CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG 
AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA 
TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB1 ( XM_004939770.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_004939827.1 Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.

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CloneID	OGa05131
Clone ID Related Accession (Same CDS sequence)	XM_004939770.2
Accession Version	XM_004939770.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3282bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	E3 ubiquitin-protein ligase MIB1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG 
CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG 
TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC 
ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC 
AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC 
GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC 
GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC 
TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG 
ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA 
TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT 
CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA 
TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC 
TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA 
GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA 
AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA 
GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT 
CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG 
TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT 
GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT 
AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT 
GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC 
TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG 
CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG 
GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA 
GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA 
TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA 
GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT 
GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG 
CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT 
CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT 
CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT 
CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA 
GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG 
TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT 
GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG 
TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC 
AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG 
AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT 
AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC 
CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA 
GATGTGGGCA AGTTAATAAT GGGACTTGGC ACTCAGGGAG CAGAGAAGAA GAGTGCAGCA 
TCTATTGCCT GCTTCCTGGC AGCCAACGGA GCCGACCTCA GCATTCGTAA TAAGAAGGGT 
CAGTCTCCTC TTGATCTCTG CCCTGACCCT AGCCTCTGCA AAGCACTGGC AAAATGCCAC 
AAAGAAAAAG TCAGTGGTCA GGTTGGTTCC CGAAGTCCAT CTATGATCAA CAATGACTCT 
GAAACCTTAG AAGAATGCAT GGTGTGTTCA GATATGAAAA GAGATACTCT GTTTGGCCCG 
TGTGGACATA TTGCCACTTG TTCTCTGTGC TCACCACGGG TGAAAAAATG CCTCATCTGT 
AAAGAACAAG TTCAGTCGAG GACAAAGATT GAAGAATGCG TAGTGTGTTC GGACAAAAAG 
GCGGCAGTCC TTTTCCAGCC TTGTGGTCAT ATGTGTGCTT GTGAGAATTG TGCAAGCTTG 
ATGAAGAAAT GTGTACAGTG TCGGGCAGTA GTAGAACGAA GAGTGCCTTT CATAATGTGC 
TGTGGAGGGA AAGGTACAGA AGATACCACT GATGATATTT CAAGTGGTAA CATTCCAGTT 
ATGCAGAAAG ACAAAGACAA CACTAACGTC AATGCAGATG TACAGAAGCT GCAGCAGCAG 
TTACAGGACA TCAAGGAACA GACTATGTGT CCTGTCTGTT TGGACCGTCT GAAGAATATG 
ATCTTTCTCT GTGGCCATGG GACGTGTCAG CTCTGTGGAG ACCGAATGAG TGAATGCCCT 
ATATGCCGCA AGGCAATCGA ACGAAGGATT CTTTTGTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004939827.1
CDS	3..3284
Translation	MRRRGGGGCRPPPVSLVPPPSRPQSPDVAAVAGRRRAARWREESGPQACARQPPRLPGVASGSPLSAQRRPAASRRVPGPIGVPVGPPRRPGPRGAGGMSSSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVASAGSAISNASGERLSQLLKKLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDVEAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGDTPLHEALRHHTLSQLRQLQDMQDVGKLIMGLGTQGAEKKSAASIACFLAANGADLSIRNKKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMINNDSETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPFIMCCGGKGTEDTTDDISSGNIPVMQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_004939770.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004939770.2

ORF Insert Sequence:

ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG 
CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG 
TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC 
ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC 
AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC 
GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC 
GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC 
TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG 
ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA 
TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT 
CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA 
TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC 
TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA 
GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA 
AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA 
GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT 
CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG 
TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT 
GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT 
AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT 
GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC 
TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG 
CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG 
GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA 
GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA 
TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA 
GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT 
GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG 
CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT 
CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT 
CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT 
CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA 
GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG 
TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT 
GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG 
TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC 
AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG 
AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT 
AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC 
CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA 
GATGTGGGCA AGTTAATAAT GGGACTTGGC ACTCAGGGAG CAGAGAAGAA GAGTGCAGCA 
TCTATTGCCT GCTTCCTGGC AGCCAACGGA GCCGACCTCA GCATTCGTAA TAAGAAGGGT 
CAGTCTCCTC TTGATCTCTG CCCTGACCCT AGCCTCTGCA AAGCACTGGC AAAATGCCAC 
AAAGAAAAAG TCAGTGGTCA GGTTGGTTCC CGAAGTCCAT CTATGATCAA CAATGACTCT 
GAAACCTTAG AAGAATGCAT GGTGTGTTCA GATATGAAAA GAGATACTCT GTTTGGCCCG 
TGTGGACATA TTGCCACTTG TTCTCTGTGC TCACCACGGG TGAAAAAATG CCTCATCTGT 
AAAGAACAAG TTCAGTCGAG GACAAAGATT GAAGAATGCG TAGTGTGTTC GGACAAAAAG 
GCGGCAGTCC TTTTCCAGCC TTGTGGTCAT ATGTGTGCTT GTGAGAATTG TGCAAGCTTG 
ATGAAGAAAT GTGTACAGTG TCGGGCAGTA GTAGAACGAA GAGTGCCTTT CATAATGTGC 
TGTGGAGGGA AAGGTACAGA AGATACCACT GATGATATTT CAAGTGGTAA CATTCCAGTT 
ATGCAGAAAG ACAAAGACAA CACTAACGTC AATGCAGATG TACAGAAGCT GCAGCAGCAG 
TTACAGGACA TCAAGGAACA GACTATGTGT CCTGTCTGTT TGGACCGTCT GAAGAATATG 
ATCTTTCTCT GTGGCCATGG GACGTGTCAG CTCTGTGGAG ACCGAATGAG TGAATGCCCT 
ATATGCCGCA AGGCAATCGA ACGAAGGATT CTTTTGTATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MIB1 cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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