mib1 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009296845.3	XP_009295120.1	E3 ubiquitin-protein ligase mib1 isoform X2
XM_009296859.3	XP_009295134.1	E3 ubiquitin-protein ligase mib1 isoform X6
XM_009296848.2	XP_009295123.1	E3 ubiquitin-protein ligase mib1 isoform X4
XM_009296846.2	XP_009295121.1	E3 ubiquitin-protein ligase mib1 isoform X3
XM_009296844.3	XP_009295119.1	E3 ubiquitin-protein ligase mib1 isoform X1
XM_009296859.2	XP_009295134.1	E3 ubiquitin-protein ligase mib1 isoform X6
XM_009296846.3	XP_009295121.1	E3 ubiquitin-protein ligase mib1 isoform X3
XM_009296845.2	XP_009295120.1	E3 ubiquitin-protein ligase mib1 isoform X2
XM_009296848.3	XP_009295123.1	E3 ubiquitin-protein ligase mib1 isoform X4
XM_009296854.3	XP_009295129.1	E3 ubiquitin-protein ligase mib1 isoform X5
XM_009296844.2	XP_009295119.1	E3 ubiquitin-protein ligase mib1 isoform X1
XM_009296854.2	XP_009295129.1	E3 ubiquitin-protein ligase mib1 isoform X5
NM_173286.3	NP_775393.2	E3 ubiquitin-protein ligase mib1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa02244	NM_173286.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40134	XM_009296844.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40135	XM_009296845.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40136	XM_009296846.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40137	XM_009296848.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40138	XM_009296854.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40139	XM_009296859.3 Latest version!	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa40139	XM_009296859.2	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40136	XM_009296846.2	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40135	XM_009296845.2	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40137	XM_009296848.2	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40134	XM_009296844.2	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa40138	XM_009296854.2	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

mib1 ( NM_173286.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_775393.2 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), mRNA.

Price & Availability↑

CloneID	ODa02244
Clone ID Related Accession (Same CDS sequence)	NM_173286.3
Accession Version	NM_173286.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF537301.1. On Jun 3, 2003 this sequence version replaced NM_173286.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF537301.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTAGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAACGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTGGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATATAATC CCGCTGCTGT CACAAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGAGC GCCTGTCTCA GCTCCTCAAG 
AAACTTTTCG AGACGCAGGA GTCTGGAGAC ATTAACGAAG AGCTGGTGAA GGCCGCAGCC 
AACGGTGACC TGGCCAAAGT AGAGGACATC CTGAAGAGAC CCGATGTGGA TGTGAACGGG 
CAGTGTGCAG GACACACTGC CATGCAGGCG GCGAGTCAAA ATGGCCATGT GGACGTCCTC 
AAACTGCTGC TGAAACATAG CGTGGATTTG GAGGCGGAGG ATAAGGATGG AGATCGAGCT 
GTCCATCACG CATCCTTCGG GGACGAGGGT TCGGTCATCG AGGTGCTTCA TCGAGGCGGT 
GCTGACCTGA ACGCCAGAAA CAAGCGCAGA CAGACGCCGC TGCACATCGC TGTCAACAAG 
GGCCATCTGC AGGTGGTCAA GACTTTGCTT GACTTTGGCT GCCACCCCAG TCTGCAGGAC 
TCTGAGGGAG ACACACCTCT GCATGATGCC ATCAGCAAGA AGCGGGATGA CATGCTCTCT 
GTGCTCCTGG AAGCAGGCGC AGACGTCACA ATCACCAACA ACAACGGTTT CAATGCGCTC 
CACCATGCAG CACTGCGAGG GAACCCGAGT GCCATGCGTG TGCTGCTTTC CAAACTGCCA 
CGGCCATGGA TAGTGGACGA GAAGAAGGAC GATGGCTACA CCGCTCTTCA TCTGGCTGCG 
CTCAACAACC ATGTGGAGGT GGCCGAGCTA CTAGTTCACC AGGGAAATGC CAATTTGGAC 
GTCCAGAATG TTAACCAGCA GACGGCATTA CACCTTGCAG TGGAGCGCCA GCACACCCAG 
ATAGTGCGAC TTTTGGTAAG AGCCGAGGCA AAGTTAGACG TTCAAGACAA GGACGGAGAC 
ACGCCACTAC ACGAGGCCCT GCGTCACCAC ACGCTGTCCC AGCTGCGCCA ACTTCAGGAC 
ATGCAGGACG TCAGCAAAGT GGAACCCTGG GAGCCCTCCA AAAACACATT GATCATGGGA 
CTGGGCACTC AGGGGGCTGA GAAGAAGAGC GCTGCCTCTA TTGCTTGCTT CCTGGCAGCC 
AACGGAGCTG ACCTTACCAT CCGTAATAAG AAGGGCCAGT CCCCTCTGGA CCTGTGTCCT 
GACCCCAGCC TCTGCAAGGC CCTAGCTAAA TGCCACAAAG AGAAGACAAG CGGGCAGGTT 
GGCTCTCGAA GCCCATCTCT AAACAGTAAT AATGAGACTC TGGAGGAATG CATGGTGTGC 
TCGGATATGA AAAGAGACAC TCTTTTTGGC CCCTGTGGTC ATATCGCCAC CTGCTCTCTC 
TGCTCGCCTC GTGTCAAGAA GTGTCTTATT TGCAAGGAAC AGGTTCAGTC CAGAACTAAG 
ATCGAGGAGT GTGTAGTTTG CTCTGATAAA AAGGCAGCGG TGCTCTTCCA GCCCTGTGGT 
CACATGTGCG CCTGTGAGAA CTGTGCAAGC CTGATGAAAA AGTGTGTGCA GTGTCGGGCC 
GTGGTGGAGC GCCGCACCCC CTTCGTTCTG TGCTGTGGAG GGAAAGGTAT GGAGGATGCC 
ACTGATGATG AGGACCTTAC AGGGGGCTCT AACTCAATGG CAGGGGGGTC GCAGGATCTT 
CTCCAGCCCA ACAATCTGGC CCTAAGTTGG TCCAGTGGAA ATATCCCAGC CTTGCAGAGG 
GACAAAGACA ACACTAATGT CAACGCAGAT GTACAGAAGC TGCAGCAGCA ACTGCAGGAT 
ATCAAAGAGC AGACTATGTG CCCGGTGTGT CTGGATCGTC TGAAGAACAT GATCTTCATG 
TGTGGCCACG GCACCTGTCA GCTGTGTGGA GACCGCATGA GCGAGTGCCC GATCTGCCGC 
AAGGCCATCG AGCGCCGAAT CCTGCTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_775393.2
CDS	100..3192
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTGGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	NM_173286.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173286.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTAGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAACGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTGGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATATAATC CCGCTGCTGT CACAAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGAGC GCCTGTCTCA GCTCCTCAAG 
AAACTTTTCG AGACGCAGGA GTCTGGAGAC ATTAACGAAG AGCTGGTGAA GGCCGCAGCC 
AACGGTGACC TGGCCAAAGT AGAGGACATC CTGAAGAGAC CCGATGTGGA TGTGAACGGG 
CAGTGTGCAG GACACACTGC CATGCAGGCG GCGAGTCAAA ATGGCCATGT GGACGTCCTC 
AAACTGCTGC TGAAACATAG CGTGGATTTG GAGGCGGAGG ATAAGGATGG AGATCGAGCT 
GTCCATCACG CATCCTTCGG GGACGAGGGT TCGGTCATCG AGGTGCTTCA TCGAGGCGGT 
GCTGACCTGA ACGCCAGAAA CAAGCGCAGA CAGACGCCGC TGCACATCGC TGTCAACAAG 
GGCCATCTGC AGGTGGTCAA GACTTTGCTT GACTTTGGCT GCCACCCCAG TCTGCAGGAC 
TCTGAGGGAG ACACACCTCT GCATGATGCC ATCAGCAAGA AGCGGGATGA CATGCTCTCT 
GTGCTCCTGG AAGCAGGCGC AGACGTCACA ATCACCAACA ACAACGGTTT CAATGCGCTC 
CACCATGCAG CACTGCGAGG GAACCCGAGT GCCATGCGTG TGCTGCTTTC CAAACTGCCA 
CGGCCATGGA TAGTGGACGA GAAGAAGGAC GATGGCTACA CCGCTCTTCA TCTGGCTGCG 
CTCAACAACC ATGTGGAGGT GGCCGAGCTA CTAGTTCACC AGGGAAATGC CAATTTGGAC 
GTCCAGAATG TTAACCAGCA GACGGCATTA CACCTTGCAG TGGAGCGCCA GCACACCCAG 
ATAGTGCGAC TTTTGGTAAG AGCCGAGGCA AAGTTAGACG TTCAAGACAA GGACGGAGAC 
ACGCCACTAC ACGAGGCCCT GCGTCACCAC ACGCTGTCCC AGCTGCGCCA ACTTCAGGAC 
ATGCAGGACG TCAGCAAAGT GGAACCCTGG GAGCCCTCCA AAAACACATT GATCATGGGA 
CTGGGCACTC AGGGGGCTGA GAAGAAGAGC GCTGCCTCTA TTGCTTGCTT CCTGGCAGCC 
AACGGAGCTG ACCTTACCAT CCGTAATAAG AAGGGCCAGT CCCCTCTGGA CCTGTGTCCT 
GACCCCAGCC TCTGCAAGGC CCTAGCTAAA TGCCACAAAG AGAAGACAAG CGGGCAGGTT 
GGCTCTCGAA GCCCATCTCT AAACAGTAAT AATGAGACTC TGGAGGAATG CATGGTGTGC 
TCGGATATGA AAAGAGACAC TCTTTTTGGC CCCTGTGGTC ATATCGCCAC CTGCTCTCTC 
TGCTCGCCTC GTGTCAAGAA GTGTCTTATT TGCAAGGAAC AGGTTCAGTC CAGAACTAAG 
ATCGAGGAGT GTGTAGTTTG CTCTGATAAA AAGGCAGCGG TGCTCTTCCA GCCCTGTGGT 
CACATGTGCG CCTGTGAGAA CTGTGCAAGC CTGATGAAAA AGTGTGTGCA GTGTCGGGCC 
GTGGTGGAGC GCCGCACCCC CTTCGTTCTG TGCTGTGGAG GGAAAGGTAT GGAGGATGCC 
ACTGATGATG AGGACCTTAC AGGGGGCTCT AACTCAATGG CAGGGGGGTC GCAGGATCTT 
CTCCAGCCCA ACAATCTGGC CCTAAGTTGG TCCAGTGGAA ATATCCCAGC CTTGCAGAGG 
GACAAAGACA ACACTAATGT CAACGCAGAT GTACAGAAGC TGCAGCAGCA ACTGCAGGAT 
ATCAAAGAGC AGACTATGTG CCCGGTGTGT CTGGATCGTC TGAAGAACAT GATCTTCATG 
TGTGGCCACG GCACCTGTCA GCTGTGTGGA GACCGCATGA GCGAGTGCCC GATCTGCCGC 
AAGGCCATCG AGCGCCGAAT CCTGCTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296844.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295119.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40134
Clone ID Related Accession (Same CDS sequence)	XM_009296844.3 , XM_009296844.2
Accession Version	XM_009296844.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296844.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACAGGGGGC 
TCTAACTCAA TGGCAGGGGG GTCGCAGGAT CTTCTCCAGC CCAACAATCT GGCCCTAAGT 
TGGTCCAGTG GAAATATCCC AGCCTTGCAG AGGGACAAAG ACAACACTAA TGTCAACGCA 
GATGTACAGA AGCTGCAACA GCAACTGCAG GATATCAAAG AGCAGACTAT GTGCCCGGTG 
TGTCTGGATC GTCTGAAGAA CATGATCTTC ATGTGTGGCC ACGGCACCTG TCAGCTGTGT 
GGAGACCGCA TGAGCGAGTG CCCGATCTGC CGCAAGGCCA TCGAGCGCCG AATCCTGCTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295119.1
CDS	1002..4127
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTGGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296844.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296844.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACAGGGGGC 
TCTAACTCAA TGGCAGGGGG GTCGCAGGAT CTTCTCCAGC CCAACAATCT GGCCCTAAGT 
TGGTCCAGTG GAAATATCCC AGCCTTGCAG AGGGACAAAG ACAACACTAA TGTCAACGCA 
GATGTACAGA AGCTGCAACA GCAACTGCAG GATATCAAAG AGCAGACTAT GTGCCCGGTG 
TGTCTGGATC GTCTGAAGAA CATGATCTTC ATGTGTGGCC ACGGCACCTG TCAGCTGTGT 
GGAGACCGCA TGAGCGAGTG CCCGATCTGC CGCAAGGCCA TCGAGCGCCG AATCCTGCTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296844.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295119.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa40134
Clone ID Related Accession (Same CDS sequence)	XM_009296844.3 , XM_009296844.2
Accession Version	XM_009296844.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACAGGGGGC 
TCTAACTCAA TGGCAGGGGG GTCGCAGGAT CTTCTCCAGC CCAACAATCT GGCCCTAAGT 
TGGTCCAGTG GAAATATCCC AGCCTTGCAG AGGGACAAAG ACAACACTAA TGTCAACGCA 
GATGTACAGA AGCTGCAACA GCAACTGCAG GATATCAAAG AGCAGACTAT GTGCCCGGTG 
TGTCTGGATC GTCTGAAGAA CATGATCTTC ATGTGTGGCC ACGGCACCTG TCAGCTGTGT 
GGAGACCGCA TGAGCGAGTG CCCGATCTGC CGCAAGGCCA TCGAGCGCCG AATCCTGCTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295119.1
CDS	1001..4126
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTGGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296844.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296844.2

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACAGGGGGC 
TCTAACTCAA TGGCAGGGGG GTCGCAGGAT CTTCTCCAGC CCAACAATCT GGCCCTAAGT 
TGGTCCAGTG GAAATATCCC AGCCTTGCAG AGGGACAAAG ACAACACTAA TGTCAACGCA 
GATGTACAGA AGCTGCAACA GCAACTGCAG GATATCAAAG AGCAGACTAT GTGCCCGGTG 
TGTCTGGATC GTCTGAAGAA CATGATCTTC ATGTGTGGCC ACGGCACCTG TCAGCTGTGT 
GGAGACCGCA TGAGCGAGTG CCCGATCTGC CGCAAGGCCA TCGAGCGCCG AATCCTGCTT 
TACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296845.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295120.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40135
Clone ID Related Accession (Same CDS sequence)	XM_009296845.3 , XM_009296845.2
Accession Version	XM_009296845.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296845.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TAGGGGCTCT 
AACTCAATGG CAGGGGGGTC GCAGGATCTT CTCCAGCCCA ACAATCTGGC CCTAAGTTGG 
TCCAGTGGAA ATATCCCAGC CTTGCAGAGG GACAAAGACA ACACTAATGT CAACGCAGAT 
GTACAGAAGC TGCAACAGCA ACTGCAGGAT ATCAAAGAGC AGACTATGTG CCCGGTGTGT 
CTGGATCGTC TGAAGAACAT GATCTTCATG TGTGGCCACG GCACCTGTCA GCTGTGTGGA 
GACCGCATGA GCGAGTGCCC GATCTGCCGC AAGGCCATCG AGCGCCGAAT CCTGCTTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295120.1
CDS	1002..4124
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLRGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296845.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296845.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TAGGGGCTCT 
AACTCAATGG CAGGGGGGTC GCAGGATCTT CTCCAGCCCA ACAATCTGGC CCTAAGTTGG 
TCCAGTGGAA ATATCCCAGC CTTGCAGAGG GACAAAGACA ACACTAATGT CAACGCAGAT 
GTACAGAAGC TGCAACAGCA ACTGCAGGAT ATCAAAGAGC AGACTATGTG CCCGGTGTGT 
CTGGATCGTC TGAAGAACAT GATCTTCATG TGTGGCCACG GCACCTGTCA GCTGTGTGGA 
GACCGCATGA GCGAGTGCCC GATCTGCCGC AAGGCCATCG AGCGCCGAAT CCTGCTTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296845.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295120.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa40135
Clone ID Related Accession (Same CDS sequence)	XM_009296845.3 , XM_009296845.2
Accession Version	XM_009296845.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3123bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TAGGGGCTCT 
AACTCAATGG CAGGGGGGTC GCAGGATCTT CTCCAGCCCA ACAATCTGGC CCTAAGTTGG 
TCCAGTGGAA ATATCCCAGC CTTGCAGAGG GACAAAGACA ACACTAATGT CAACGCAGAT 
GTACAGAAGC TGCAACAGCA ACTGCAGGAT ATCAAAGAGC AGACTATGTG CCCGGTGTGT 
CTGGATCGTC TGAAGAACAT GATCTTCATG TGTGGCCACG GCACCTGTCA GCTGTGTGGA 
GACCGCATGA GCGAGTGCCC GATCTGCCGC AAGGCCATCG AGCGCCGAAT CCTGCTTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295120.1
CDS	1002..4124
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLRGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296845.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296845.2

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TAGGGGCTCT 
AACTCAATGG CAGGGGGGTC GCAGGATCTT CTCCAGCCCA ACAATCTGGC CCTAAGTTGG 
TCCAGTGGAA ATATCCCAGC CTTGCAGAGG GACAAAGACA ACACTAATGT CAACGCAGAT 
GTACAGAAGC TGCAACAGCA ACTGCAGGAT ATCAAAGAGC AGACTATGTG CCCGGTGTGT 
CTGGATCGTC TGAAGAACAT GATCTTCATG TGTGGCCACG GCACCTGTCA GCTGTGTGGA 
GACCGCATGA GCGAGTGCCC GATCTGCCGC AAGGCCATCG AGCGCCGAAT CCTGCTTTAC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296846.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295121.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa40136
Clone ID Related Accession (Same CDS sequence)	XM_009296846.2 , XM_009296846.3
Accession Version	XM_009296846.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC AGGGGGCTCT AACTCAATGG CAGGGGGGTC GCAGGATCTT 
CTCCAGCCCA ACAATCTGGC CCTAAGTTGG TCCAGTGGAA ATATCCCAGC CTTGCAGAGG 
GACAAAGACA ACACTAATGT CAACGCAGAT GTACAGAAGC TGCAACAGCA ACTGCAGGAT 
ATCAAAGAGC AGACTATGTG CCCGGTGTGT CTGGATCGTC TGAAGAACAT GATCTTCATG 
TGTGGCCACG GCACCTGTCA GCTGTGTGGA GACCGCATGA GCGAGTGCCC GATCTGCCGC 
AAGGCCATCG AGCGCCGAAT CCTGCTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295121.1
CDS	1002..4094
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKAGGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296846.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296846.2

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC AGGGGGCTCT AACTCAATGG CAGGGGGGTC GCAGGATCTT 
CTCCAGCCCA ACAATCTGGC CCTAAGTTGG TCCAGTGGAA ATATCCCAGC CTTGCAGAGG 
GACAAAGACA ACACTAATGT CAACGCAGAT GTACAGAAGC TGCAACAGCA ACTGCAGGAT 
ATCAAAGAGC AGACTATGTG CCCGGTGTGT CTGGATCGTC TGAAGAACAT GATCTTCATG 
TGTGGCCACG GCACCTGTCA GCTGTGTGGA GACCGCATGA GCGAGTGCCC GATCTGCCGC 
AAGGCCATCG AGCGCCGAAT CCTGCTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296846.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295121.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X3, mRNA.

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CloneID	ODa40136
Clone ID Related Accession (Same CDS sequence)	XM_009296846.2 , XM_009296846.3
Accession Version	XM_009296846.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296846.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC AGGGGGCTCT AACTCAATGG CAGGGGGGTC GCAGGATCTT 
CTCCAGCCCA ACAATCTGGC CCTAAGTTGG TCCAGTGGAA ATATCCCAGC CTTGCAGAGG 
GACAAAGACA ACACTAATGT CAACGCAGAT GTACAGAAGC TGCAACAGCA ACTGCAGGAT 
ATCAAAGAGC AGACTATGTG CCCGGTGTGT CTGGATCGTC TGAAGAACAT GATCTTCATG 
TGTGGCCACG GCACCTGTCA GCTGTGTGGA GACCGCATGA GCGAGTGCCC GATCTGCCGC 
AAGGCCATCG AGCGCCGAAT CCTGCTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295121.1
CDS	1002..4094
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKAGGSNSMAGGSQDLLQPNNLALSWSSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296846.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296846.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC AGGGGGCTCT AACTCAATGG CAGGGGGGTC GCAGGATCTT 
CTCCAGCCCA ACAATCTGGC CCTAAGTTGG TCCAGTGGAA ATATCCCAGC CTTGCAGAGG 
GACAAAGACA ACACTAATGT CAACGCAGAT GTACAGAAGC TGCAACAGCA ACTGCAGGAT 
ATCAAAGAGC AGACTATGTG CCCGGTGTGT CTGGATCGTC TGAAGAACAT GATCTTCATG 
TGTGGCCACG GCACCTGTCA GCTGTGTGGA GACCGCATGA GCGAGTGCCC GATCTGCCGC 
AAGGCCATCG AGCGCCGAAT CCTGCTTTAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296848.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295123.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa40137
Clone ID Related Accession (Same CDS sequence)	XM_009296848.2 , XM_009296848.3
Accession Version	XM_009296848.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACCAGTGGA 
AATATCCCAG CCTTGCAGAG GGACAAAGAC AACACTAATG TCAACGCAGA TGTACAGAAG 
CTGCAACAGC AACTGCAGGA TATCAAAGAG CAGACTATGT GCCCGGTGTG TCTGGATCGT 
CTGAAGAACA TGATCTTCAT GTGTGGCCAC GGCACCTGTC AGCTGTGTGG AGACCGCATG 
AGCGAGTGCC CGATCTGCCG CAAGGCCATC GAGCGCCGAA TCCTGCTTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295123.1
CDS	1002..4055
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296848.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296848.2

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACCAGTGGA 
AATATCCCAG CCTTGCAGAG GGACAAAGAC AACACTAATG TCAACGCAGA TGTACAGAAG 
CTGCAACAGC AACTGCAGGA TATCAAAGAG CAGACTATGT GCCCGGTGTG TCTGGATCGT 
CTGAAGAACA TGATCTTCAT GTGTGGCCAC GGCACCTGTC AGCTGTGTGG AGACCGCATG 
AGCGAGTGCC CGATCTGCCG CAAGGCCATC GAGCGCCGAA TCCTGCTTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296848.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295123.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa40137
Clone ID Related Accession (Same CDS sequence)	XM_009296848.2 , XM_009296848.3
Accession Version	XM_009296848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACCAGTGGA 
AATATCCCAG CCTTGCAGAG GGACAAAGAC AACACTAATG TCAACGCAGA TGTACAGAAG 
CTGCAACAGC AACTGCAGGA TATCAAAGAG CAGACTATGT GCCCGGTGTG TCTGGATCGT 
CTGAAGAACA TGATCTTCAT GTGTGGCCAC GGCACCTGTC AGCTGTGTGG AGACCGCATG 
AGCGAGTGCC CGATCTGCCG CAAGGCCATC GAGCGCCGAA TCCTGCTTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295123.1
CDS	1002..4055
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296848.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296848.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGG TATGGAGGAT GCCACTGATG ATGAGGACCT TACCAGTGGA 
AATATCCCAG CCTTGCAGAG GGACAAAGAC AACACTAATG TCAACGCAGA TGTACAGAAG 
CTGCAACAGC AACTGCAGGA TATCAAAGAG CAGACTATGT GCCCGGTGTG TCTGGATCGT 
CTGAAGAACA TGATCTTCAT GTGTGGCCAC GGCACCTGTC AGCTGTGTGG AGACCGCATG 
AGCGAGTGCC CGATCTGCCG CAAGGCCATC GAGCGCCGAA TCCTGCTTTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296854.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295129.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa40138
Clone ID Related Accession (Same CDS sequence)	XM_009296854.3 , XM_009296854.2
Accession Version	XM_009296854.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGAGC GCCTGTCTCA GCTCCTCAAG 
AAACTTTTCG AGACGCAGGA GTCTGGAGAC ATTAACGAAG AGCTGGTGAA GGCCGCAGCC 
AACGGTGACC TGGCCAAAGT AGAGGACATC CTGAAGAGAC CCGATGTGGA TGTGAACGGG 
CAGTGTGCAG GACACACTGC CATGCAGGCG GCGAGTCAAA ATGGCCATGT GGACGTCCTC 
AAACTGCTGC TGAAACATAG CGTGGATTTG GAGGCGGAGG ATAAGGATGG AGATCGAGCT 
GTCCATCACG CATCCTTCGG GGACGAGGGT TCGGTCATCG AGGTGCTTCA TCGAGGCGGT 
GCTGACCTGA ACGCCAGAAA CAAGCGCAGA CAGACGCCGC TGCACATCGC TGTCAACAAG 
GGCCATCTGC AGGTGGTCAA GACTTTGCTT GACTTTGGCT GCCACCCCAG TCTGCAGGAC 
TCTGAGGGAG ACACACCTCT GCATGATGCC ATCAGCAAGA AGCGGGATGA CATGCTCTCT 
GTGCTCCTGG AAGCAGGCGC AGACGTCACA ATCACCAACA ACAACGGTTT CAATGCGCTC 
CACCATGCAG CACTGCGAGG GAACCCGAGT GCCATGCGTG TGCTGCTTTC CAAACTGCCA 
CGGCCATGGA TAGTGGACGA GAAGAAGGAC GATGGCTACA CCGCTCTTCA TCTGGCTGCG 
CTCAACAACC ATGTGGAGGT GGCCGAGCTA CTAGTTCACC AGGGAAATGC CAATTTGGAC 
GTCCAGAATG TTAACCAGCA GACGGCATTA CACCTTGCAG TGGAGCGCCA GCACACCCAG 
ATAGTGCGAC TTTTGGTAAG AGCCGAGGCA AAGTTAGACG TTCAAGACAA GGACGGAGAC 
ACGCCACTGC ACGAGGCCCT GCGTCACCAC ACGCTGTCCC AGCTGCGCCA ACTTCAGGAC 
ATGCAGGACG TCAGCAAAGT GGAACCCTGG GAGCCCTCCA AAAACACATT GATCATGGGA 
CTGGGCACTC AGGGGGCTGA GAAGAAGAGC GCTGCCTCTA TTGCTTGCTT CCTGGCAGCC 
AACGGAGCTG ACCTTACCAT CCGTAATAAG AAGGGCCAGT CCCCTCTGGA CCTGTGTCCT 
GACCCCAGCC TCTGCAAGGC CCTAGCTAAA TGCCACAAAG AGAAGACAAG CGGGCAGGTT 
GGCTCTCGAA GCCCGTCTCT AAACAGTAAT AATGAGACTC TGGAGGAATG CATGGTGTGC 
TCGGATATGA AAAGAGACAC TCTTTTTGGC CCCTGTGGTC ATATCGCCAC CTGCTCTCTC 
TGCTCGCCTC GTGTCAAGAA GTGTCTTATT TGCAAGGAAC AGGTTCAGTC CAGAACTAAG 
ATCGAGGAGT GTGTAGTTTG CTCTGATAAA AAGGCAGCGG TGCTCTTCCA GCCCTGTGGT 
CACATGTGCG CCTGTGAGAA CTGTGCGAGT CTGATGAAAA AGTGTGTGCA GTGTCGGGCC 
GTGGTGGAGC GCCGCACCCC CTTCGTTCTG TGCTGTGGAG GGAAAGGTAT GGAGGATGCC 
ACTGATGATG AGGACCTTAC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295129.1
CDS	1002..4022
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296854.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296854.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGAGC GCCTGTCTCA GCTCCTCAAG 
AAACTTTTCG AGACGCAGGA GTCTGGAGAC ATTAACGAAG AGCTGGTGAA GGCCGCAGCC 
AACGGTGACC TGGCCAAAGT AGAGGACATC CTGAAGAGAC CCGATGTGGA TGTGAACGGG 
CAGTGTGCAG GACACACTGC CATGCAGGCG GCGAGTCAAA ATGGCCATGT GGACGTCCTC 
AAACTGCTGC TGAAACATAG CGTGGATTTG GAGGCGGAGG ATAAGGATGG AGATCGAGCT 
GTCCATCACG CATCCTTCGG GGACGAGGGT TCGGTCATCG AGGTGCTTCA TCGAGGCGGT 
GCTGACCTGA ACGCCAGAAA CAAGCGCAGA CAGACGCCGC TGCACATCGC TGTCAACAAG 
GGCCATCTGC AGGTGGTCAA GACTTTGCTT GACTTTGGCT GCCACCCCAG TCTGCAGGAC 
TCTGAGGGAG ACACACCTCT GCATGATGCC ATCAGCAAGA AGCGGGATGA CATGCTCTCT 
GTGCTCCTGG AAGCAGGCGC AGACGTCACA ATCACCAACA ACAACGGTTT CAATGCGCTC 
CACCATGCAG CACTGCGAGG GAACCCGAGT GCCATGCGTG TGCTGCTTTC CAAACTGCCA 
CGGCCATGGA TAGTGGACGA GAAGAAGGAC GATGGCTACA CCGCTCTTCA TCTGGCTGCG 
CTCAACAACC ATGTGGAGGT GGCCGAGCTA CTAGTTCACC AGGGAAATGC CAATTTGGAC 
GTCCAGAATG TTAACCAGCA GACGGCATTA CACCTTGCAG TGGAGCGCCA GCACACCCAG 
ATAGTGCGAC TTTTGGTAAG AGCCGAGGCA AAGTTAGACG TTCAAGACAA GGACGGAGAC 
ACGCCACTGC ACGAGGCCCT GCGTCACCAC ACGCTGTCCC AGCTGCGCCA ACTTCAGGAC 
ATGCAGGACG TCAGCAAAGT GGAACCCTGG GAGCCCTCCA AAAACACATT GATCATGGGA 
CTGGGCACTC AGGGGGCTGA GAAGAAGAGC GCTGCCTCTA TTGCTTGCTT CCTGGCAGCC 
AACGGAGCTG ACCTTACCAT CCGTAATAAG AAGGGCCAGT CCCCTCTGGA CCTGTGTCCT 
GACCCCAGCC TCTGCAAGGC CCTAGCTAAA TGCCACAAAG AGAAGACAAG CGGGCAGGTT 
GGCTCTCGAA GCCCGTCTCT AAACAGTAAT AATGAGACTC TGGAGGAATG CATGGTGTGC 
TCGGATATGA AAAGAGACAC TCTTTTTGGC CCCTGTGGTC ATATCGCCAC CTGCTCTCTC 
TGCTCGCCTC GTGTCAAGAA GTGTCTTATT TGCAAGGAAC AGGTTCAGTC CAGAACTAAG 
ATCGAGGAGT GTGTAGTTTG CTCTGATAAA AAGGCAGCGG TGCTCTTCCA GCCCTGTGGT 
CACATGTGCG CCTGTGAGAA CTGTGCGAGT CTGATGAAAA AGTGTGTGCA GTGTCGGGCC 
GTGGTGGAGC GCCGCACCCC CTTCGTTCTG TGCTGTGGAG GGAAAGGTAT GGAGGATGCC 
ACTGATGATG AGGACCTTAC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296854.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295129.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa40138
Clone ID Related Accession (Same CDS sequence)	XM_009296854.3 , XM_009296854.2
Accession Version	XM_009296854.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGAGC GCCTGTCTCA GCTCCTCAAG 
AAACTTTTCG AGACGCAGGA GTCTGGAGAC ATTAACGAAG AGCTGGTGAA GGCCGCAGCC 
AACGGTGACC TGGCCAAAGT AGAGGACATC CTGAAGAGAC CCGATGTGGA TGTGAACGGG 
CAGTGTGCAG GACACACTGC CATGCAGGCG GCGAGTCAAA ATGGCCATGT GGACGTCCTC 
AAACTGCTGC TGAAACATAG CGTGGATTTG GAGGCGGAGG ATAAGGATGG AGATCGAGCT 
GTCCATCACG CATCCTTCGG GGACGAGGGT TCGGTCATCG AGGTGCTTCA TCGAGGCGGT 
GCTGACCTGA ACGCCAGAAA CAAGCGCAGA CAGACGCCGC TGCACATCGC TGTCAACAAG 
GGCCATCTGC AGGTGGTCAA GACTTTGCTT GACTTTGGCT GCCACCCCAG TCTGCAGGAC 
TCTGAGGGAG ACACACCTCT GCATGATGCC ATCAGCAAGA AGCGGGATGA CATGCTCTCT 
GTGCTCCTGG AAGCAGGCGC AGACGTCACA ATCACCAACA ACAACGGTTT CAATGCGCTC 
CACCATGCAG CACTGCGAGG GAACCCGAGT GCCATGCGTG TGCTGCTTTC CAAACTGCCA 
CGGCCATGGA TAGTGGACGA GAAGAAGGAC GATGGCTACA CCGCTCTTCA TCTGGCTGCG 
CTCAACAACC ATGTGGAGGT GGCCGAGCTA CTAGTTCACC AGGGAAATGC CAATTTGGAC 
GTCCAGAATG TTAACCAGCA GACGGCATTA CACCTTGCAG TGGAGCGCCA GCACACCCAG 
ATAGTGCGAC TTTTGGTAAG AGCCGAGGCA AAGTTAGACG TTCAAGACAA GGACGGAGAC 
ACGCCACTGC ACGAGGCCCT GCGTCACCAC ACGCTGTCCC AGCTGCGCCA ACTTCAGGAC 
ATGCAGGACG TCAGCAAAGT GGAACCCTGG GAGCCCTCCA AAAACACATT GATCATGGGA 
CTGGGCACTC AGGGGGCTGA GAAGAAGAGC GCTGCCTCTA TTGCTTGCTT CCTGGCAGCC 
AACGGAGCTG ACCTTACCAT CCGTAATAAG AAGGGCCAGT CCCCTCTGGA CCTGTGTCCT 
GACCCCAGCC TCTGCAAGGC CCTAGCTAAA TGCCACAAAG AGAAGACAAG CGGGCAGGTT 
GGCTCTCGAA GCCCGTCTCT AAACAGTAAT AATGAGACTC TGGAGGAATG CATGGTGTGC 
TCGGATATGA AAAGAGACAC TCTTTTTGGC CCCTGTGGTC ATATCGCCAC CTGCTCTCTC 
TGCTCGCCTC GTGTCAAGAA GTGTCTTATT TGCAAGGAAC AGGTTCAGTC CAGAACTAAG 
ATCGAGGAGT GTGTAGTTTG CTCTGATAAA AAGGCAGCGG TGCTCTTCCA GCCCTGTGGT 
CACATGTGCG CCTGTGAGAA CTGTGCGAGT CTGATGAAAA AGTGTGTGCA GTGTCGGGCC 
GTGGTGGAGC GCCGCACCCC CTTCGTTCTG TGCTGTGGAG GGAAAGGTAT GGAGGATGCC 
ACTGATGATG AGGACCTTAC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295129.1
CDS	1001..4021
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKGMEDATDDEDLTSGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296854.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296854.2

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGAGC GCCTGTCTCA GCTCCTCAAG 
AAACTTTTCG AGACGCAGGA GTCTGGAGAC ATTAACGAAG AGCTGGTGAA GGCCGCAGCC 
AACGGTGACC TGGCCAAAGT AGAGGACATC CTGAAGAGAC CCGATGTGGA TGTGAACGGG 
CAGTGTGCAG GACACACTGC CATGCAGGCG GCGAGTCAAA ATGGCCATGT GGACGTCCTC 
AAACTGCTGC TGAAACATAG CGTGGATTTG GAGGCGGAGG ATAAGGATGG AGATCGAGCT 
GTCCATCACG CATCCTTCGG GGACGAGGGT TCGGTCATCG AGGTGCTTCA TCGAGGCGGT 
GCTGACCTGA ACGCCAGAAA CAAGCGCAGA CAGACGCCGC TGCACATCGC TGTCAACAAG 
GGCCATCTGC AGGTGGTCAA GACTTTGCTT GACTTTGGCT GCCACCCCAG TCTGCAGGAC 
TCTGAGGGAG ACACACCTCT GCATGATGCC ATCAGCAAGA AGCGGGATGA CATGCTCTCT 
GTGCTCCTGG AAGCAGGCGC AGACGTCACA ATCACCAACA ACAACGGTTT CAATGCGCTC 
CACCATGCAG CACTGCGAGG GAACCCGAGT GCCATGCGTG TGCTGCTTTC CAAACTGCCA 
CGGCCATGGA TAGTGGACGA GAAGAAGGAC GATGGCTACA CCGCTCTTCA TCTGGCTGCG 
CTCAACAACC ATGTGGAGGT GGCCGAGCTA CTAGTTCACC AGGGAAATGC CAATTTGGAC 
GTCCAGAATG TTAACCAGCA GACGGCATTA CACCTTGCAG TGGAGCGCCA GCACACCCAG 
ATAGTGCGAC TTTTGGTAAG AGCCGAGGCA AAGTTAGACG TTCAAGACAA GGACGGAGAC 
ACGCCACTGC ACGAGGCCCT GCGTCACCAC ACGCTGTCCC AGCTGCGCCA ACTTCAGGAC 
ATGCAGGACG TCAGCAAAGT GGAACCCTGG GAGCCCTCCA AAAACACATT GATCATGGGA 
CTGGGCACTC AGGGGGCTGA GAAGAAGAGC GCTGCCTCTA TTGCTTGCTT CCTGGCAGCC 
AACGGAGCTG ACCTTACCAT CCGTAATAAG AAGGGCCAGT CCCCTCTGGA CCTGTGTCCT 
GACCCCAGCC TCTGCAAGGC CCTAGCTAAA TGCCACAAAG AGAAGACAAG CGGGCAGGTT 
GGCTCTCGAA GCCCGTCTCT AAACAGTAAT AATGAGACTC TGGAGGAATG CATGGTGTGC 
TCGGATATGA AAAGAGACAC TCTTTTTGGC CCCTGTGGTC ATATCGCCAC CTGCTCTCTC 
TGCTCGCCTC GTGTCAAGAA GTGTCTTATT TGCAAGGAAC AGGTTCAGTC CAGAACTAAG 
ATCGAGGAGT GTGTAGTTTG CTCTGATAAA AAGGCAGCGG TGCTCTTCCA GCCCTGTGGT 
CACATGTGCG CCTGTGAGAA CTGTGCGAGT CTGATGAAAA AGTGTGTGCA GTGTCGGGCC 
GTGGTGGAGC GCCGCACCCC CTTCGTTCTG TGCTGTGGAG GGAAAGGTAT GGAGGATGCC 
ACTGATGATG AGGACCTTAC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296859.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295134.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa40139
Clone ID Related Accession (Same CDS sequence)	XM_009296859.3 , XM_009296859.2
Accession Version	XM_009296859.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296859.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295134.1
CDS	1002..4022
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKASGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296859.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296859.3

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib1 ( XM_009296859.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295134.1 Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa40139
Clone ID Related Accession (Same CDS sequence)	XM_009296859.3 , XM_009296859.2
Accession Version	XM_009296859.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	E3 ubiquitin-protein ligase mib1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296859.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295134.1
CDS	1002..4022
Translation	MTTGRNNRVMMEGVGARVIRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDVRILDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTTCYHGDKHHLRHRFYRITTPGSERVLLESRRKSKKITARGIFAGGRVVRGVDWQWEDQDGGNGRRGKVTEIQDWSAASPHSAAYVLWDNGAKNLYRVGFEGMSDLKCVQDAKGGTFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLTTTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANVVRSGEVAAGAEGGSSQFMVGDLVQICYDIDRIKLLQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVTKVAPAGSAVTNASGAVLTESPSVPAERLSQLLKKLFETQESGDINEELVKAAANGDLAKVEDILKRPDVDVNGQCAGHTAMQAASQNGHVDVLKLLLKHSVDLEAEDKDGDRAVHHASFGDEGSVIEVLHRGGADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQDSEGDTPLHDAISKKRDDMLSVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKDDGYTALHLAALNNHVEVAELLVHQGNANLDVQNVNQQTALHLAVERQHTQIVRLLVRAEAKLDVQDKDGDTPLHEALRHHTLSQLRQLQDMQDVSKVEPWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLTIRNKKGQSPLDLCPDPSLCKALAKCHKEKTSGQVGSRSPSLNSNNETLEECMVCSDMKRDTLFGPCGHIATCSLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRTPFVLCCGGKASGNIPALQRDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFMCGHGTCQLCGDRMSECPICRKAIERRILLY

Target ORF information:

RefSeq Version	XM_009296859.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mindbomb E3 ubiquitin protein ligase 1 (mib1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296859.2

ORF Insert Sequence:

ATGACCACCG GCAGGAATAA CCGAGTGATG ATGGAAGGAG TGGGTGCTCG AGTTATCCGC 
GGGCCTGACT GGAAGTGGGG GAAGCAGGAC GGCGGAGAAG GGCATGTGGG CACCGTCAGG 
AGCTTCGAGA GCCCGGAGGA GGTGGTGGTG GTGTGGGATA ACGGCACCGC CGCCAATTAC 
CGCTGCTCCG GGGCTTATGA TGTGAGGATA TTGGACAGTG CGCCTACAGG AATCAAGCAT 
GATGGCACCA TGTGTGACAC CTGTCGGCAG CAGCCCATCA TTGGAATCCG ATGGAAATGT 
GCAGAGTGCA CCAACTACGA CCTCTGTACC ACCTGTTACC ATGGCGACAA GCACCACCTG 
CGCCACAGGT TTTACCGCAT CACCACCCCT GGCAGTGAAA GAGTTCTTCT GGAGTCTCGT 
CGCAAGTCGA AGAAGATCAC AGCCCGCGGC ATCTTTGCAG GAGGACGAGT GGTGCGAGGG 
GTGGATTGGC AATGGGAGGA TCAGGATGGC GGGAATGGAA GAAGAGGGAA GGTGACGGAG 
ATCCAGGACT GGAGTGCTGC CAGCCCCCAC AGCGCAGCCT ACGTCCTTTG GGACAACGGG 
GCCAAGAACC TCTACCGAGT GGGATTCGAG GGCATGTCTG ATTTGAAATG TGTGCAAGAT 
GCCAAAGGAG GGACTTTTTA CAGAGACCAC TGTCCAGTAT TGGGTGAGCA GAACGGCAAC 
AGGAACCCAG GAGGCCTGCA GATCGGAGAC TTGGTCAACA TTGATTTGGA CCTGGAGATC 
GTTCAGTCAC TGCAGCATGG GCATGGAGGA TGGACCGACG GCATGTTCGA GACGCTCACC 
ACCACAGGCA CTGTCTGCGG CATCGATGAA GACCACGACA TTGTTGTGCA GTATCCTAGC 
GGAAACAGGT GGACGTTTAA CCCAGCGGTG CTGACCAAGG CAAATGTTGT CCGCAGTGGA 
GAAGTGGCAG CTGGCGCTGA GGGCGGCAGC TCGCAGTTCA TGGTGGGAGA TTTAGTCCAG 
ATCTGCTACG ACATCGACCG CATCAAACTG CTCCAGAGAG GACACGGAGA GTGGGCCGAA 
GCCATGCTGC CAACCCTTGG AAAGGTGGGC AGAGTGCAGC AGATTTACTC TGACAGTGAC 
CTGAAAGTGG AGGTGTGCGG GACGTCCTGG ACATACAATC CCGCTGCTGT CACTAAAGTG 
GCCCCTGCTG GATCTGCGGT TACTAATGCT TCTGGAGCAG TGTTGACAGA GAGTCCTTCT 
GTGCCCGCAG AGCGCCTGTC TCAGCTCCTC AAGAAACTTT TCGAGACGCA GGAGTCTGGA 
GACATTAACG AAGAGCTGGT GAAGGCCGCA GCCAACGGTG ACCTGGCCAA AGTAGAGGAC 
ATCCTGAAGA GACCCGATGT GGATGTGAAC GGGCAGTGTG CAGGACACAC TGCCATGCAG 
GCGGCGAGTC AAAATGGCCA TGTGGACGTC CTCAAACTGC TGCTGAAACA TAGCGTGGAT 
TTGGAGGCGG AGGATAAGGA TGGAGATCGA GCTGTCCATC ACGCATCCTT CGGGGACGAG 
GGTTCGGTCA TCGAGGTGCT TCATCGAGGC GGTGCTGACC TGAACGCCAG AAACAAGCGC 
AGACAGACGC CGCTGCACAT CGCTGTCAAC AAGGGCCATC TGCAGGTGGT CAAGACTTTG 
CTTGACTTTG GCTGCCACCC CAGTCTGCAG GACTCTGAGG GAGACACACC TCTGCATGAT 
GCCATCAGCA AGAAGCGGGA TGACATGCTC TCTGTGCTCC TGGAAGCAGG CGCAGACGTC 
ACAATCACCA ACAACAACGG TTTCAATGCG CTCCACCATG CAGCACTGCG AGGGAACCCG 
AGTGCCATGC GTGTGCTGCT TTCCAAACTG CCACGGCCAT GGATAGTGGA CGAGAAGAAG 
GACGATGGCT ACACCGCTCT TCATCTGGCT GCGCTCAACA ACCATGTGGA GGTGGCCGAG 
CTACTAGTTC ACCAGGGAAA TGCCAATTTG GACGTCCAGA ATGTTAACCA GCAGACGGCA 
TTACACCTTG CAGTGGAGCG CCAGCACACC CAGATAGTGC GACTTTTGGT AAGAGCCGAG 
GCAAAGTTAG ACGTTCAAGA CAAGGACGGA GACACGCCAC TGCACGAGGC CCTGCGTCAC 
CACACGCTGT CCCAGCTGCG CCAACTTCAG GACATGCAGG ACGTCAGCAA AGTGGAACCC 
TGGGAGCCCT CCAAAAACAC ATTGATCATG GGACTGGGCA CTCAGGGGGC TGAGAAGAAG 
AGCGCTGCCT CTATTGCTTG CTTCCTGGCA GCCAACGGAG CTGACCTTAC CATCCGTAAT 
AAGAAGGGCC AGTCCCCTCT GGACCTGTGT CCTGACCCCA GCCTCTGCAA GGCCCTAGCT 
AAATGCCACA AAGAGAAGAC AAGCGGGCAG GTTGGCTCTC GAAGCCCGTC TCTAAACAGT 
AATAATGAGA CTCTGGAGGA ATGCATGGTG TGCTCGGATA TGAAAAGAGA CACTCTTTTT 
GGCCCCTGTG GTCATATCGC CACCTGCTCT CTCTGCTCGC CTCGTGTCAA GAAGTGTCTT 
ATTTGCAAGG AACAGGTTCA GTCCAGAACT AAGATCGAGG AGTGTGTAGT TTGCTCTGAT 
AAAAAGGCAG CGGTGCTCTT CCAGCCCTGT GGTCACATGT GCGCCTGTGA GAACTGTGCG 
AGTCTGATGA AAAAGTGTGT GCAGTGTCGG GCCGTGGTGG AGCGCCGCAC CCCCTTCGTT 
CTGTGCTGTG GAGGGAAAGC CAGTGGAAAT ATCCCAGCCT TGCAGAGGGA CAAAGACAAC 
ACTAATGTCA ACGCAGATGT ACAGAAGCTG CAACAGCAAC TGCAGGATAT CAAAGAGCAG 
ACTATGTGCC CGGTGTGTCT GGATCGTCTG AAGAACATGA TCTTCATGTG TGGCCACGGC 
ACCTGTCAGC TGTGTGGAGA CCGCATGAGC GAGTGCCCGA TCTGCCGCAA GGCCATCGAG 
CGCCGAATCC TGCTTTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	mib1
Entrez Gene ID	352910
Full Name	mindbomb E3 ubiquitin protein ligase 1
Synonyms	cg5841,chunp6889,fe47f05,im:7148100,mib,wu:fe47f05
General protein information	Preferred Names mindbomb E3 ubiquitin protein ligase 1 Names E3 ubiquitin-protein ligase mib1 RING-type E3 ubiquitin transferase mib1 mind bomb protein mind bomb white tail wit
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	MIB1	NP_065825.1
Mus musculus (house mouse)	Mib1	NP_659109.2
Gallus gallus (chicken)	MIB1	XP_419157.3
Danio rerio (zebrafish)	mib	NP_775393.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011932	XP_320601.4
Canis lupus familiaris (dog)	MIB1	XP_547643.4
Drosophila melanogaster (fruit fly)	mib1	NP_648826.2
Xenopus tropicalis (tropical clawed frog)	mib1	NP_001123407.1
Pan troglodytes (chimpanzee)	MIB1	XP_003315915.1
	MIB1	XP_001092086.1
Bos taurus (cattle)	MIB1	NP_001192959.1
Rattus norvegicus (Norway rat)	Mib1	NP_001100875.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

mib1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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