MIB1 cDNA ORF clone, Gallus gallus(chicken)

The following MIB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa146349 XM_004939769.3
Latest version!
Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa146350 XM_004939770.3
Latest version!
Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OGa146348 XM_419157.6
Latest version!
Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OGa05129 XM_419157.5
Latest version!
Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OGa05130 XM_004939769.2
Latest version!
Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OGa05131 XM_004939770.2
Latest version!
Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa146349
    Clone ID Related Accession (Same CDS sequence) XM_004939769.3
    Accession Version XM_004939769.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase MIB1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
    GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC
    AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC
    CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT
    GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT
    GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA
    AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC
    CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT
    GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA
    ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT
    GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT
    GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC
    AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT
    GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA
    ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT
    GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA
    GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA
    GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA
    GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC
    TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG
    GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG
    AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC
    AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA
    CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG
    AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT
    GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC
    GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA
    GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT
    TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT
    GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT
    CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA
    CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT
    CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT
    ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG
    ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT
    ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC
    ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG
    GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA
    GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC
    CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG
    GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG
    GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT
    TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG
    ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT
    TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT
    CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGCAAGTGGT
    AACATTCCAG TTATGCAGAA AGACAAAGAC AACACTAACG TCAATGCAGA TGTACAGAAG
    CTGCAGCAGC AGTTACAGGA CATCAAGGAA CAGACTATGT GTCCTGTCTG TTTGGACCGT
    CTGAAGAATA TGATCTTTCT CTGTGGCCAT GGGACGTGTC AGCTCTGTGG AGACCGAATG
    AGTGAATGCC CTATATGCCG CAAGGCAATC GAACGAAGGA TTCTTTTGTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939826.2
    CDS294..3287
    Translation

    Target ORF information:

    RefSeq Version XM_004939769.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939769.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
    GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC
    AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC
    CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT
    GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT
    GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA
    AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC
    CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT
    GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA
    ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT
    GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT
    GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC
    AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT
    GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA
    ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT
    GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA
    GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA
    GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA
    GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC
    TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG
    GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG
    AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC
    AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA
    CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG
    AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT
    GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC
    GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA
    GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT
    TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT
    GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT
    CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA
    CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT
    CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT
    ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG
    ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT
    ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC
    ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG
    GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA
    GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC
    CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG
    GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG
    GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT
    TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG
    ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT
    TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT
    CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGCAAGTGGT
    AACATTCCAG TTATGCAGAA AGACAAAGAC AACACTAACG TCAATGCAGA TGTACAGAAG
    CTGCAGCAGC AGTTACAGGA CATCAAGGAA CAGACTATGT GTCCTGTCTG TTTGGACCGT
    CTGAAGAATA TGATCTTTCT CTGTGGCCAT GGGACGTGTC AGCTCTGTGG AGACCGAATG
    AGTGAATGCC CTATATGCCG CAAGGCAATC GAACGAAGGA TTCTTTTGTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146350
    Clone ID Related Accession (Same CDS sequence) XM_004939770.3
    Accession Version XM_004939770.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2988bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase MIB1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004939770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
    GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC
    AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC
    CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT
    GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT
    GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA
    AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC
    CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT
    GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA
    ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT
    GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT
    GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC
    AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT
    GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA
    ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT
    GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA
    GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA
    GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA
    GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC
    TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG
    GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG
    AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC
    AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA
    CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG
    AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT
    GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC
    GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA
    GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT
    TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT
    GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT
    CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA
    CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT
    CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT
    ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG
    ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT
    ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC
    ATGCAAGATG TGGGCAAGTT AATAATGGGA CTTGGCACTC AGGGAGCAGA GAAGAAGAGT
    GCAGCATCTA TTGCCTGCTT CCTGGCAGCC AACGGAGCCG ACCTCAGCAT TCGTAATAAG
    AAGGGTCAGT CTCCTCTTGA TCTCTGCCCT GACCCTAGCC TCTGCAAAGC ACTGGCAAAA
    TGCCACAAAG AAAAAGTCAG TGGTCAGGTT GGTTCCCGAA GTCCATCTAT GATCAACAAT
    GACTCTGAAA CCTTAGAAGA ATGCATGGTG TGTTCAGATA TGAAAAGAGA TACTCTGTTT
    GGCCCGTGTG GACATATTGC CACTTGTTCT CTGTGCTCAC CACGGGTGAA AAAATGCCTC
    ATCTGTAAAG AACAAGTTCA GTCGAGGACA AAGATTGAAG AATGCGTAGT GTGTTCGGAC
    AAAAAGGCGG CAGTCCTTTT CCAGCCTTGT GGTCATATGT GTGCTTGTGA GAATTGTGCA
    AGCTTGATGA AGAAATGTGT ACAGTGTCGG GCAGTAGTAG AACGAAGAGT GCCTTTCATA
    ATGTGCTGTG GAGGGAAAGG TACAGAAGAT ACCACTGATG ATATTTCAAG TGGTAACATT
    CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG
    CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG
    AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA
    TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939827.2
    CDS294..3281
    Translation

    Target ORF information:

    RefSeq Version XM_004939770.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939770.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
    GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC
    AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC
    CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT
    GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT
    GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA
    AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC
    CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT
    GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA
    ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT
    GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT
    GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC
    AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT
    GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA
    ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT
    GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA
    GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA
    GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA
    GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC
    TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG
    GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG
    AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC
    AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA
    CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG
    AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT
    GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC
    GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA
    GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT
    TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT
    GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT
    CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA
    CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT
    CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT
    ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG
    ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT
    ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC
    ATGCAAGATG TGGGCAAGTT AATAATGGGA CTTGGCACTC AGGGAGCAGA GAAGAAGAGT
    GCAGCATCTA TTGCCTGCTT CCTGGCAGCC AACGGAGCCG ACCTCAGCAT TCGTAATAAG
    AAGGGTCAGT CTCCTCTTGA TCTCTGCCCT GACCCTAGCC TCTGCAAAGC ACTGGCAAAA
    TGCCACAAAG AAAAAGTCAG TGGTCAGGTT GGTTCCCGAA GTCCATCTAT GATCAACAAT
    GACTCTGAAA CCTTAGAAGA ATGCATGGTG TGTTCAGATA TGAAAAGAGA TACTCTGTTT
    GGCCCGTGTG GACATATTGC CACTTGTTCT CTGTGCTCAC CACGGGTGAA AAAATGCCTC
    ATCTGTAAAG AACAAGTTCA GTCGAGGACA AAGATTGAAG AATGCGTAGT GTGTTCGGAC
    AAAAAGGCGG CAGTCCTTTT CCAGCCTTGT GGTCATATGT GTGCTTGTGA GAATTGTGCA
    AGCTTGATGA AGAAATGTGT ACAGTGTCGG GCAGTAGTAG AACGAAGAGT GCCTTTCATA
    ATGTGCTGTG GAGGGAAAGG TACAGAAGAT ACCACTGATG ATATTTCAAG TGGTAACATT
    CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG
    CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG
    AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA
    TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa146348
    Clone ID Related Accession (Same CDS sequence) XM_419157.6
    Accession Version XM_419157.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3021bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase MIB1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006089.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_419157.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
    GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC
    AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC
    CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT
    GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT
    GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA
    AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC
    CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT
    GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA
    ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT
    GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT
    GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC
    AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT
    GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA
    ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT
    GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA
    GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA
    GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA
    GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC
    TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG
    GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG
    AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC
    AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA
    CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG
    AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT
    GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC
    GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA
    GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT
    TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT
    GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT
    CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA
    CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT
    CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT
    ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG
    ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT
    ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC
    ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG
    GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA
    GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC
    CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG
    GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG
    GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT
    TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG
    ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT
    TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT
    CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGGTACAGAA
    GATACCACTG ATGATATTTC AAGTGGTAAC ATTCCAGTTA TGCAGAAAGA CAAAGACAAC
    ACTAACGTCA ATGCAGATGT ACAGAAGCTG CAGCAGCAGT TACAGGACAT CAAGGAACAG
    ACTATGTGTC CTGTCTGTTT GGACCGTCTG AAGAATATGA TCTTTCTCTG TGGCCATGGG
    ACGTGTCAGC TCTGTGGAGA CCGAATGAGT GAATGCCCTA TATGCCGCAA GGCAATCGAA
    CGAAGGATTC TTTTGTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_419157.4
    CDS294..3314
    Translation

    Target ORF information:

    RefSeq Version XM_419157.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_419157.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGCAGCT CGAGGAACAA CCGCGTGATG GTGGAGGGCG TCGGGGCCCG CGTGGTGCGC 
    GGCCCCGACT GGAAGTGGGG AAAGCAGGAC GGCGGCGAGG GCCACGTGGG CACCGTGCGC
    AGCTTCGAGA GCCCCGAGGA GGTGGTGGTG GTGTGGGACA ACGGCACCGC CGCCAACTAC
    CGCTGCTCCG GGGCCTACGA CCTCCGCATC CTGGACAGCG CGCCCACCGG TATCAAGCAT
    GATGGGACTA TGTGTGACAC ATGTCGACAG CAGCCAATCA TTGGCATCCG ATGGAAATGT
    GCTGAATGCA CGAATTACGA CTTGTGCACA GTTTGTTATC ATGGGGACAA GCATCACTTA
    AGGCATCGTT TTTACAGAAT TACCACACCA GGAAGTGAAA GGGTATTGCT GGAGTCTCGC
    CGTAAATCAA AGAAAATTAC AGCAAGAGGA ATCTTTGCAG GTGCCAGAGT GGTGCGAGGT
    GTTGACTGGC AATGGGAAGA TCAAGATGGT GGCAATGGGC GCAGGGGAAA GGTAACTGAA
    ATCCAAGATT GGAGTGCGTC AAGTCCACAT AGTGCAGCAT ACGTTCTTTG GGACAATGGT
    GCTAAAAACC TTTACAGAGT TGGCTTTGAG GGCATGTCTG ACCTGAAATG CGTCCAAGAT
    GCAAAAGGAG GCTCTTTCTA CAGAGACCAC TGTCCTGTGT TAGGTGAGCA AAATGGTAAC
    AGAAATCCTG GTGGTCTGCA AATTGGTGAC CTTGTAAACA TTGACCTTGA TTTGGAAATT
    GTACAGTCTT TGCAGCACGG TCATGGAGGA TGGACTGATG GCATGTTTGA AACCTTAACA
    ACAACTGGAA CAGTTTGTGG CATTGATGAG GACCATGACA TTGTAGTACA ATATCCAAGT
    GGCAATAGAT GGACATTTAA TCCAGCTGTT CTCACCAAAG CCAATGTTGT CCGAAGTGGA
    GATGCTGCTC AAGGTGCAGA AGGAGGTACT TCTCAGTTTC AAGTGGGTGA CCTTGTTCAA
    GTGTGCTATG ACTTGGAGCG AATCAAGCTT CTTCAGAGGG GTCATGGAGA GTGGGCTGAA
    GCAATGCTTC CTACTTTAGG TAAAGTTGGT CGTGTCCAGC AGATCTATTC GGACAGTGAC
    TTAAAGGTAG AGGTTTGTGG GACATCTTGG ACATACAATC CAGCAGCTGT CTCAAAGGTG
    GCATCAGCAG GATCTGCTAT AAGTAATGCG TCTGGCGAGA GACTGTCCCA GCTACTGAAG
    AAATTATTTG AAACCCAAGA ATCTGGAGAT CTCAATGAAG AATTGGTTAA AGCTGCTGCC
    AATGGAGATG TTGCTAAAGT GGAAGATCTG CTAAAGAGAC CAGACGTGGA TGTAAATGGA
    CAGTGTGCTG GACACACAGC TATGCAAGCA GCTAGTCAGA ATGGCCACGT TGACATTTTG
    AAGTTGCTTC TGAAACAGAA TGTGGATGTA GAAGCAGAGG ATAAAGATGG AGACAGGGCT
    GTCCATCACG CAGCCTTTGG CGATGAGGGT GCTGTGATAG AGGTTTTGCA CCGAGGCAGC
    GCAGATCTGA ACGCTCGCAA CAAACGGAGG CAGACTCCAC TTCATATTGC TGTCAACAAA
    GGTCATCTGC AAGTTGTGAA GACTTTACTG GACTTCGGCT GCCATCCTAG TCTCCAGGAT
    TCTGAAGGTG ACACTCCGCT TCATGATGCA ATAAGTAAAA AGCGTGACGA CATCCTGGCT
    GTACTGTTAG AAGCTGGTGC AGATGTTACT ATCACCAACA ACAATGGCTT TAATGCTCTT
    CACCATGCTG CGCTAAGAGG AAACCCAAGT GCAATGCGTG TATTGCTGTC CAAGTTGCCA
    CGACCGTGGA TTGTTGATGA GAAAAAAGAT GATGGTTACA CTGCCCTGCA TCTGGCAGCT
    CTCAACAATC ACGTGGAGGT GGCTGAACTT TTGGTGCATC AGGGCAACGC TAACCTGGAT
    ATCCAGAATG TTAACCAGCA AACTGCTCTG CACCTTGCTG TTGAGCGTCA GCATACACAG
    ATTGTTAGGC TCTTAGTCCG TGCAGGTGCT AAGCTGGATA TCCAGGATAA AGATGGGGAT
    ACTCCCCTGC ATGAAGCCCT GCGACACCAC ACCCTGTCAC AGCTTCGCCA GCTCCAAGAC
    ATGCAAGATG TGGGCAAGGT AGATACTGCT TGGGAGCCAT CAAAGAACAC ATTAATAATG
    GGACTTGGCA CTCAGGGAGC AGAGAAGAAG AGTGCAGCAT CTATTGCCTG CTTCCTGGCA
    GCCAACGGAG CCGACCTCAG CATTCGTAAT AAGAAGGGTC AGTCTCCTCT TGATCTCTGC
    CCTGACCCTA GCCTCTGCAA AGCACTGGCA AAATGCCACA AAGAAAAAGT CAGTGGTCAG
    GTTGGTTCCC GAAGTCCATC TATGATCAAC AATGACTCTG AAACCTTAGA AGAATGCATG
    GTGTGTTCAG ATATGAAAAG AGATACTCTG TTTGGCCCGT GTGGACATAT TGCCACTTGT
    TCTCTGTGCT CACCACGGGT GAAAAAATGC CTCATCTGTA AAGAACAAGT TCAGTCGAGG
    ACAAAGATTG AAGAATGCGT AGTGTGTTCG GACAAAAAGG CGGCAGTCCT TTTCCAGCCT
    TGTGGTCATA TGTGTGCTTG TGAGAATTGT GCAAGCTTGA TGAAGAAATG TGTACAGTGT
    CGGGCAGTAG TAGAACGAAG AGTGCCTTTC ATAATGTGCT GTGGAGGGAA AGGTACAGAA
    GATACCACTG ATGATATTTC AAGTGGTAAC ATTCCAGTTA TGCAGAAAGA CAAAGACAAC
    ACTAACGTCA ATGCAGATGT ACAGAAGCTG CAGCAGCAGT TACAGGACAT CAAGGAACAG
    ACTATGTGTC CTGTCTGTTT GGACCGTCTG AAGAATATGA TCTTTCTCTG TGGCCATGGG
    ACGTGTCAGC TCTGTGGAGA CCGAATGAGT GAATGCCCTA TATGCCGCAA GGCAATCGAA
    CGAAGGATTC TTTTGTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa05129
    Clone ID Related Accession (Same CDS sequence) XM_419157.5
    Accession Version XM_419157.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3315bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase MIB1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_419157.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
    TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG
    CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG
    TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC
    ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC
    AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC
    GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC
    GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC
    TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG
    ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA
    TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT
    CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA
    TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC
    TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA
    GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA
    AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA
    GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT
    CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG
    TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT
    GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT
    AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT
    GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC
    TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG
    CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG
    GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA
    GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA
    TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA
    GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT
    GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG
    CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT
    CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT
    CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT
    CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA
    GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG
    TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT
    GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG
    TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC
    AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG
    AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT
    AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC
    CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA
    GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT
    GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC
    GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC
    CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT
    TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT
    TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG
    TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG
    ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT
    CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA
    GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGGTAC AGAAGATACC
    ACTGATGATA TTTCAAGTGG TAACATTCCA GTTATGCAGA AAGACAAAGA CAACACTAAC
    GTCAATGCAG ATGTACAGAA GCTGCAGCAG CAGTTACAGG ACATCAAGGA ACAGACTATG
    TGTCCTGTCT GTTTGGACCG TCTGAAGAAT ATGATCTTTC TCTGTGGCCA TGGGACGTGT
    CAGCTCTGTG GAGACCGAAT GAGTGAATGC CCTATATGCC GCAAGGCAAT CGAACGAAGG
    ATTCTTTTGT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_419157.3
    CDS3..3317
    Translation

    Target ORF information:

    RefSeq Version XM_419157.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_419157.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
    TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG
    CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG
    TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC
    ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC
    AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC
    GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC
    GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC
    TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG
    ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA
    TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT
    CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA
    TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC
    TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA
    GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA
    AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA
    GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT
    CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG
    TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT
    GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT
    AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT
    GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC
    TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG
    CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG
    GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA
    GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA
    TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA
    GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT
    GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG
    CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT
    CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT
    CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT
    CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA
    GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG
    TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT
    GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG
    TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC
    AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG
    AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT
    AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC
    CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA
    GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT
    GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC
    GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC
    CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT
    TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT
    TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG
    TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG
    ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT
    CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA
    GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGGTAC AGAAGATACC
    ACTGATGATA TTTCAAGTGG TAACATTCCA GTTATGCAGA AAGACAAAGA CAACACTAAC
    GTCAATGCAG ATGTACAGAA GCTGCAGCAG CAGTTACAGG ACATCAAGGA ACAGACTATG
    TGTCCTGTCT GTTTGGACCG TCTGAAGAAT ATGATCTTTC TCTGTGGCCA TGGGACGTGT
    CAGCTCTGTG GAGACCGAAT GAGTGAATGC CCTATATGCC GCAAGGCAAT CGAACGAAGG
    ATTCTTTTGT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa05130
    Clone ID Related Accession (Same CDS sequence) XM_004939769.2
    Accession Version XM_004939769.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3288bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase MIB1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
    TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG
    CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG
    TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC
    ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC
    AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC
    GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC
    GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC
    TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG
    ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA
    TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT
    CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA
    TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC
    TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA
    GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA
    AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA
    GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT
    CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG
    TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT
    GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT
    AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT
    GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC
    TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG
    CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG
    GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA
    GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA
    TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA
    GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT
    GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG
    CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT
    CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT
    CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT
    CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA
    GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG
    TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT
    GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG
    TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC
    AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG
    AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT
    AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC
    CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA
    GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT
    GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC
    GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC
    CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT
    TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT
    TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG
    TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG
    ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT
    CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA
    GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGCAAG TGGTAACATT
    CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG
    CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG
    AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA
    TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939826.1
    CDS3..3290
    Translation

    Target ORF information:

    RefSeq Version XM_004939769.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
    TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG
    CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG
    TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC
    ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC
    AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC
    GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC
    GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC
    TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG
    ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA
    TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT
    CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA
    TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC
    TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA
    GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA
    AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA
    GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT
    CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG
    TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT
    GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT
    AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT
    GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC
    TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG
    CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG
    GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA
    GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA
    TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA
    GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT
    GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG
    CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT
    CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT
    CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT
    CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA
    GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG
    TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT
    GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG
    TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC
    AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG
    AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT
    AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC
    CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA
    GATGTGGGCA AGGTAGATAC TGCTTGGGAG CCATCAAAGA ACACATTAAT AATGGGACTT
    GGCACTCAGG GAGCAGAGAA GAAGAGTGCA GCATCTATTG CCTGCTTCCT GGCAGCCAAC
    GGAGCCGACC TCAGCATTCG TAATAAGAAG GGTCAGTCTC CTCTTGATCT CTGCCCTGAC
    CCTAGCCTCT GCAAAGCACT GGCAAAATGC CACAAAGAAA AAGTCAGTGG TCAGGTTGGT
    TCCCGAAGTC CATCTATGAT CAACAATGAC TCTGAAACCT TAGAAGAATG CATGGTGTGT
    TCAGATATGA AAAGAGATAC TCTGTTTGGC CCGTGTGGAC ATATTGCCAC TTGTTCTCTG
    TGCTCACCAC GGGTGAAAAA ATGCCTCATC TGTAAAGAAC AAGTTCAGTC GAGGACAAAG
    ATTGAAGAAT GCGTAGTGTG TTCGGACAAA AAGGCGGCAG TCCTTTTCCA GCCTTGTGGT
    CATATGTGTG CTTGTGAGAA TTGTGCAAGC TTGATGAAGA AATGTGTACA GTGTCGGGCA
    GTAGTAGAAC GAAGAGTGCC TTTCATAATG TGCTGTGGAG GGAAAGCAAG TGGTAACATT
    CCAGTTATGC AGAAAGACAA AGACAACACT AACGTCAATG CAGATGTACA GAAGCTGCAG
    CAGCAGTTAC AGGACATCAA GGAACAGACT ATGTGTCCTG TCTGTTTGGA CCGTCTGAAG
    AATATGATCT TTCTCTGTGG CCATGGGACG TGTCAGCTCT GTGGAGACCG AATGAGTGAA
    TGCCCTATAT GCCGCAAGGC AATCGAACGA AGGATTCTTT TGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa05131
    Clone ID Related Accession (Same CDS sequence) XM_004939770.2
    Accession Version XM_004939770.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3282bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase MIB1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003763686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004939770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
    TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG
    CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG
    TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC
    ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC
    AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC
    GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC
    GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC
    TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG
    ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA
    TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT
    CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA
    TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC
    TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA
    GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA
    AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA
    GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT
    CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG
    TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT
    GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT
    AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT
    GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC
    TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG
    CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG
    GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA
    GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA
    TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA
    GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT
    GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG
    CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT
    CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT
    CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT
    CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA
    GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG
    TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT
    GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG
    TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC
    AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG
    AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT
    AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC
    CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA
    GATGTGGGCA AGTTAATAAT GGGACTTGGC ACTCAGGGAG CAGAGAAGAA GAGTGCAGCA
    TCTATTGCCT GCTTCCTGGC AGCCAACGGA GCCGACCTCA GCATTCGTAA TAAGAAGGGT
    CAGTCTCCTC TTGATCTCTG CCCTGACCCT AGCCTCTGCA AAGCACTGGC AAAATGCCAC
    AAAGAAAAAG TCAGTGGTCA GGTTGGTTCC CGAAGTCCAT CTATGATCAA CAATGACTCT
    GAAACCTTAG AAGAATGCAT GGTGTGTTCA GATATGAAAA GAGATACTCT GTTTGGCCCG
    TGTGGACATA TTGCCACTTG TTCTCTGTGC TCACCACGGG TGAAAAAATG CCTCATCTGT
    AAAGAACAAG TTCAGTCGAG GACAAAGATT GAAGAATGCG TAGTGTGTTC GGACAAAAAG
    GCGGCAGTCC TTTTCCAGCC TTGTGGTCAT ATGTGTGCTT GTGAGAATTG TGCAAGCTTG
    ATGAAGAAAT GTGTACAGTG TCGGGCAGTA GTAGAACGAA GAGTGCCTTT CATAATGTGC
    TGTGGAGGGA AAGGTACAGA AGATACCACT GATGATATTT CAAGTGGTAA CATTCCAGTT
    ATGCAGAAAG ACAAAGACAA CACTAACGTC AATGCAGATG TACAGAAGCT GCAGCAGCAG
    TTACAGGACA TCAAGGAACA GACTATGTGT CCTGTCTGTT TGGACCGTCT GAAGAATATG
    ATCTTTCTCT GTGGCCATGG GACGTGTCAG CTCTGTGGAG ACCGAATGAG TGAATGCCCT
    ATATGCCGCA AGGCAATCGA ACGAAGGATT CTTTTGTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004939827.1
    CDS3..3284
    Translation

    Target ORF information:

    RefSeq Version XM_004939770.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus mindbomb E3 ubiquitin protein ligase 1 (MIB1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004939770.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCGCAGGC GGGGAGGCGG GGGCTGCCGG CCGCCGCCGG TCTCCCTGGT GCCGCCGCCG 
    TCGCGGCCGC AGTCCCCGGA TGTAGCGGCG GTAGCGGGCC GGCGGCGGGC GGCCCGTTGG
    CGGGAGGAGA GCGGCCCGCA GGCCTGTGCC CGGCAGCCGC CGCGTTTGCC CGGCGTCGCG
    TCGGGATCGC CTCTCTCGGC GCAGCGGCGG CCCGCGGCCT CCCGCCGCGT TCCCGGCCCC
    ATCGGCGTCC CCGTGGGGCC GCCCCGCCGG CCGGGCCCGC GCGGCGCCGG CGGGATGAGC
    AGCTCGAGGA ACAACCGCGT GATGGTGGAG GGCGTCGGGG CCCGCGTGGT GCGCGGCCCC
    GACTGGAAGT GGGGAAAGCA GGACGGCGGC GAGGGCCACG TGGGCACCGT GCGCAGCTTC
    GAGAGCCCCG AGGAGGTGGT GGTGGTGTGG GACAACGGCA CCGCCGCCAA CTACCGCTGC
    TCCGGGGCCT ACGACCTCCG CATCCTGGAC AGCGCGCCCA CCGGTATCAA GCATGATGGG
    ACTATGTGTG ACACATGTCG ACAGCAGCCA ATCATTGGCA TCCGATGGAA ATGTGCTGAA
    TGCACGAATT ACGACTTGTG CACAGTTTGT TATCATGGGG ACAAGCATCA CTTAAGGCAT
    CGTTTTTACA GAATTACCAC ACCAGGAAGT GAAAGGGTAT TGCTGGAGTC TCGCCGTAAA
    TCAAAGAAAA TTACAGCAAG AGGAATCTTT GCAGGTGCCA GAGTGGTGCG AGGTGTTGAC
    TGGCAATGGG AAGATCAAGA TGGTGGCAAT GGGCGCAGGG GAAAGGTAAC TGAAATCCAA
    GATTGGAGTG CGTCAAGTCC ACATAGTGCA GCATACGTTC TTTGGGACAA TGGTGCTAAA
    AACCTTTACA GAGTTGGCTT TGAGGGCATG TCTGACCTGA AATGCGTCCA AGATGCAAAA
    GGAGGCTCTT TCTACAGAGA CCACTGTCCT GTGTTAGGTG AGCAAAATGG TAACAGAAAT
    CCTGGTGGTC TGCAAATTGG TGACCTTGTA AACATTGACC TTGATTTGGA AATTGTACAG
    TCTTTGCAGC ACGGTCATGG AGGATGGACT GATGGCATGT TTGAAACCTT AACAACAACT
    GGAACAGTTT GTGGCATTGA TGAGGACCAT GACATTGTAG TACAATATCC AAGTGGCAAT
    AGATGGACAT TTAATCCAGC TGTTCTCACC AAAGCCAATG TTGTCCGAAG TGGAGATGCT
    GCTCAAGGTG CAGAAGGAGG TACTTCTCAG TTTCAAGTGG GTGACCTTGT TCAAGTGTGC
    TATGACTTGG AGCGAATCAA GCTTCTTCAG AGGGGTCATG GAGAGTGGGC TGAAGCAATG
    CTTCCTACTT TAGGTAAAGT TGGTCGTGTC CAGCAGATCT ATTCGGACAG TGACTTAAAG
    GTAGAGGTTT GTGGGACATC TTGGACATAC AATCCAGCAG CTGTCTCAAA GGTGGCATCA
    GCAGGATCTG CTATAAGTAA TGCGTCTGGC GAGAGACTGT CCCAGCTACT GAAGAAATTA
    TTTGAAACCC AAGAATCTGG AGATCTCAAT GAAGAATTGG TTAAAGCTGC TGCCAATGGA
    GATGTTGCTA AAGTGGAAGA TCTGCTAAAG AGACCAGACG TGGATGTAAA TGGACAGTGT
    GCTGGACACA CAGCTATGCA AGCAGCTAGT CAGAATGGCC ACGTTGACAT TTTGAAGTTG
    CTTCTGAAAC AGAATGTGGA TGTAGAAGCA GAGGATAAAG ATGGAGACAG GGCTGTCCAT
    CACGCAGCCT TTGGCGATGA GGGTGCTGTG ATAGAGGTTT TGCACCGAGG CAGCGCAGAT
    CTGAACGCTC GCAACAAACG GAGGCAGACT CCACTTCATA TTGCTGTCAA CAAAGGTCAT
    CTGCAAGTTG TGAAGACTTT ACTGGACTTC GGCTGCCATC CTAGTCTCCA GGATTCTGAA
    GGTGACACTC CGCTTCATGA TGCAATAAGT AAAAAGCGTG ACGACATCCT GGCTGTACTG
    TTAGAAGCTG GTGCAGATGT TACTATCACC AACAACAATG GCTTTAATGC TCTTCACCAT
    GCTGCGCTAA GAGGAAACCC AAGTGCAATG CGTGTATTGC TGTCCAAGTT GCCACGACCG
    TGGATTGTTG ATGAGAAAAA AGATGATGGT TACACTGCCC TGCATCTGGC AGCTCTCAAC
    AATCACGTGG AGGTGGCTGA ACTTTTGGTG CATCAGGGCA ACGCTAACCT GGATATCCAG
    AATGTTAACC AGCAAACTGC TCTGCACCTT GCTGTTGAGC GTCAGCATAC ACAGATTGTT
    AGGCTCTTAG TCCGTGCAGG TGCTAAGCTG GATATCCAGG ATAAAGATGG GGATACTCCC
    CTGCATGAAG CCCTGCGACA CCACACCCTG TCACAGCTTC GCCAGCTCCA AGACATGCAA
    GATGTGGGCA AGTTAATAAT GGGACTTGGC ACTCAGGGAG CAGAGAAGAA GAGTGCAGCA
    TCTATTGCCT GCTTCCTGGC AGCCAACGGA GCCGACCTCA GCATTCGTAA TAAGAAGGGT
    CAGTCTCCTC TTGATCTCTG CCCTGACCCT AGCCTCTGCA AAGCACTGGC AAAATGCCAC
    AAAGAAAAAG TCAGTGGTCA GGTTGGTTCC CGAAGTCCAT CTATGATCAA CAATGACTCT
    GAAACCTTAG AAGAATGCAT GGTGTGTTCA GATATGAAAA GAGATACTCT GTTTGGCCCG
    TGTGGACATA TTGCCACTTG TTCTCTGTGC TCACCACGGG TGAAAAAATG CCTCATCTGT
    AAAGAACAAG TTCAGTCGAG GACAAAGATT GAAGAATGCG TAGTGTGTTC GGACAAAAAG
    GCGGCAGTCC TTTTCCAGCC TTGTGGTCAT ATGTGTGCTT GTGAGAATTG TGCAAGCTTG
    ATGAAGAAAT GTGTACAGTG TCGGGCAGTA GTAGAACGAA GAGTGCCTTT CATAATGTGC
    TGTGGAGGGA AAGGTACAGA AGATACCACT GATGATATTT CAAGTGGTAA CATTCCAGTT
    ATGCAGAAAG ACAAAGACAA CACTAACGTC AATGCAGATG TACAGAAGCT GCAGCAGCAG
    TTACAGGACA TCAAGGAACA GACTATGTGT CCTGTCTGTT TGGACCGTCT GAAGAATATG
    ATCTTTCTCT GTGGCCATGG GACGTGTCAG CTCTGTGGAG ACCGAATGAG TGAATGCCCT
    ATATGCCGCA AGGCAATCGA ACGAAGGATT CTTTTGTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.