DGKI cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	418124
Full Name	diacylglycerol kinase iota
General protein information	Preferred Names diacylglycerol kinase iota Names diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015286157.2	XP_015141643.2	diacylglycerol kinase iota isoform X1
XM_025156057.1	XP_025011825.1	diacylglycerol kinase iota isoform X6
XM_025156046.1	XP_025011814.1	diacylglycerol kinase iota isoform X2
XM_025156051.1	XP_025011819.1	diacylglycerol kinase iota isoform X4
XM_025156048.1	XP_025011816.1	diacylglycerol kinase iota isoform X3
XM_015286157.1	XP_015141643.1	diacylglycerol kinase iota
XM_025156054.1	XP_025011822.1	diacylglycerol kinase iota isoform X5
XM_025156061.1	XP_025011829.1	diacylglycerol kinase iota isoform X7

Pathways from BioSystems

Homologies

	DGKI	XP_001107030.1
Canis lupus familiaris (dog)	DGKI	XP_005629798.1
Bos taurus (cattle)	DGKI	XP_002687107.1
Rattus norvegicus (Norway rat)	Dgki	NP_942077.2
Danio rerio (zebrafish)	dgki	XP_002667022.3
Xenopus tropicalis (tropical clawed frog)	dgki	XP_002942047.2
Gallus gallus (chicken)	DGKI	XP_416357.4
Homo sapiens (human)	DGKI	NP_004708.1
Pan troglodytes (chimpanzee)	DGKI	XP_001147695.1
Mus musculus (house mouse)	Dgki	NP_001074675.1

Gene Ontology

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa142742	XM_015286157.2 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa142743	XM_025156046.1 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa142744	XM_025156048.1 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa142745	XM_025156051.1 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa142746	XM_025156054.1 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa142747	XM_025156057.1 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OGa142748	XM_025156061.1 Latest version!	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OGa29385	XM_015286157.1 Latest version!	Gallus gallus diacylglycerol kinase, iota (DGKI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_015286157.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141643.2 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa142742
Clone ID Related Accession (Same CDS sequence)	XM_015286157.2
Accession Version	XM_015286157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2670bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015286157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GGGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GGACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA TGATAACAAA GGTCGGCCAT TCGTGATAAA GCCTATTTCC 
TCTCCACTCA TGAAACCACT GTTGGTTTTT GTAAATCCAA AAAGTGGAGG GAACCAGGGC 
ACCAAAGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAAAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGTGGCG GGGATGGAAC GGTTGGCTGG ATCCTCTCTA TCCTAGATGA GCTGCAACTC 
ACTCCTCAGC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GGAATGACCT TGCCCGCACC 
CTCAACTGGG GTGGGGGTTA CACAGATGAG CCTGTGTCCA AGATCTTGTG TCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGATGG AATCTACAAG TTGAGCGCAA CCCAGATTTG 
CCACAGGATG AGCTGGAAGA TGGGTCACGT AAGCTTCCAC TCAATGTCTT CAATAATTAC 
TTCAGCCTTG GGTTTGATGC ACATGTTACA CTGGAGTTCC ACGAATCCAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCAGGGGC AGCCTTTTCA 
GACTTCTTGC AAAGAAGCTC AAGAGATTTA TCCAAGCACG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATT 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAA 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAAG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACGCTTTTA 
ACGTACAAGT CTATCCCCAT GCAAGTGGAT GGTGAACCTT GTCGGTTGGC TCCCTCACTC 
ATTCGCATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTGT TGAATGATAT CCATAAAGTG CAGTCTGCTG ACCTGAGGAG AGTCTCAGCT 
CCCCCCGATT CCTTCACTGT TCCTCATTCC ATCCCTGATC GCCTGCGGAT CAGAGTGAAC 
CGGATTAATT TGCAAGAATA TGAGGGGCTA CATTATGACA AGGAAAAACT CCGTGAAGCT 
TCCATTCCCT TAGGAATTAT AGTTGTACGA GGAGATTGTG ACTTGGAGAC CTGTCGTATG 
TATATTGACC GTCTACAAGA GGATCTACAG TCAGCAACTT CTACTACACA GAGAGTTCAT 
TACCAGGACC AAGAAAGCTC TTTCCCAAGA GTACTTTCTG TTCAGAGGCT CTCTCCTAGA 
TGGTGCTTCC TAGATGCAAC TTCTGCTGAT CGTTTTTACC GCATTGACAG ATCTCAGGAA 
CATTTGCACT TTGTGACGGA AATTTCGCAG GACGAGATTT TTATTCTGGA CCCAGAGCTG 
GTAATGTCAC AGCAGGGGGG GACGCCACCT GGAATGCCTG ATCTGGTAGT GGAGCAGGCC 
TCTGGAATAT CAGATCGGTG GAACCCTGCT GTTCGAAAGA GGATGCTGAG CGACAGTGGC 
CTTGGCAAGA TTTCCCCACA CTACGAGGTA CCTGACAAAC AAAAGGACGC CAGCCAGACC 
TATATACTGC AGTCACCAGT TTCTTCAGAA GATCAAGCAC TTTTACAGGC AGTCATAGCT 
GGTGATCTGC TGAAGTTCAT AGAATGCTGT AAAAAAGGAG CCAATTTGCT AATCCAAGGA 
CCTGACCACG GCTCCCTGTT GCACCACGCT GCCAAGAGTG GGCATGGCGA GATTGTGAAG 
TATATCCTGG AACATGGTCC ATCTGAATTA CTGGACATGA CAGACAGTGA AACGGGTGAG 
ACAGCATTAC ATAAAGCGGC CTGCCAACGC CATCGTGCCA TCTGCCAGCT CCTGGTGGAT 
GCGGGAGCCT CGCTGAGAAA GACGGACTCC AAGGGTAAGA CTCCTCGTGA CAGGGCTCAG 
CAAGCTGGTG ATCCAGATCT GGCCTCATAT CTGGAGAGTC GACAGAATTA CCAAATGGTT 
CCCCATGAGG ATTTGGAAAC AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141643.2
CDS	394..3063
Translation	MEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQGVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDIHKVQSADLRRVSAPPDSFTVPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_015286157.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286157.2

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GGGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GGACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA TGATAACAAA GGTCGGCCAT TCGTGATAAA GCCTATTTCC 
TCTCCACTCA TGAAACCACT GTTGGTTTTT GTAAATCCAA AAAGTGGAGG GAACCAGGGC 
ACCAAAGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAAAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGTGGCG GGGATGGAAC GGTTGGCTGG ATCCTCTCTA TCCTAGATGA GCTGCAACTC 
ACTCCTCAGC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GGAATGACCT TGCCCGCACC 
CTCAACTGGG GTGGGGGTTA CACAGATGAG CCTGTGTCCA AGATCTTGTG TCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGATGG AATCTACAAG TTGAGCGCAA CCCAGATTTG 
CCACAGGATG AGCTGGAAGA TGGGTCACGT AAGCTTCCAC TCAATGTCTT CAATAATTAC 
TTCAGCCTTG GGTTTGATGC ACATGTTACA CTGGAGTTCC ACGAATCCAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCAGGGGC AGCCTTTTCA 
GACTTCTTGC AAAGAAGCTC AAGAGATTTA TCCAAGCACG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATT 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAA 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAAG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACGCTTTTA 
ACGTACAAGT CTATCCCCAT GCAAGTGGAT GGTGAACCTT GTCGGTTGGC TCCCTCACTC 
ATTCGCATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTGT TGAATGATAT CCATAAAGTG CAGTCTGCTG ACCTGAGGAG AGTCTCAGCT 
CCCCCCGATT CCTTCACTGT TCCTCATTCC ATCCCTGATC GCCTGCGGAT CAGAGTGAAC 
CGGATTAATT TGCAAGAATA TGAGGGGCTA CATTATGACA AGGAAAAACT CCGTGAAGCT 
TCCATTCCCT TAGGAATTAT AGTTGTACGA GGAGATTGTG ACTTGGAGAC CTGTCGTATG 
TATATTGACC GTCTACAAGA GGATCTACAG TCAGCAACTT CTACTACACA GAGAGTTCAT 
TACCAGGACC AAGAAAGCTC TTTCCCAAGA GTACTTTCTG TTCAGAGGCT CTCTCCTAGA 
TGGTGCTTCC TAGATGCAAC TTCTGCTGAT CGTTTTTACC GCATTGACAG ATCTCAGGAA 
CATTTGCACT TTGTGACGGA AATTTCGCAG GACGAGATTT TTATTCTGGA CCCAGAGCTG 
GTAATGTCAC AGCAGGGGGG GACGCCACCT GGAATGCCTG ATCTGGTAGT GGAGCAGGCC 
TCTGGAATAT CAGATCGGTG GAACCCTGCT GTTCGAAAGA GGATGCTGAG CGACAGTGGC 
CTTGGCAAGA TTTCCCCACA CTACGAGGTA CCTGACAAAC AAAAGGACGC CAGCCAGACC 
TATATACTGC AGTCACCAGT TTCTTCAGAA GATCAAGCAC TTTTACAGGC AGTCATAGCT 
GGTGATCTGC TGAAGTTCAT AGAATGCTGT AAAAAAGGAG CCAATTTGCT AATCCAAGGA 
CCTGACCACG GCTCCCTGTT GCACCACGCT GCCAAGAGTG GGCATGGCGA GATTGTGAAG 
TATATCCTGG AACATGGTCC ATCTGAATTA CTGGACATGA CAGACAGTGA AACGGGTGAG 
ACAGCATTAC ATAAAGCGGC CTGCCAACGC CATCGTGCCA TCTGCCAGCT CCTGGTGGAT 
GCGGGAGCCT CGCTGAGAAA GACGGACTCC AAGGGTAAGA CTCCTCGTGA CAGGGCTCAG 
CAAGCTGGTG ATCCAGATCT GGCCTCATAT CTGGAGAGTC GACAGAATTA CCAAATGGTT 
CCCCATGAGG ATTTGGAAAC AGCAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_025156046.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011814.1 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa142743
Clone ID Related Accession (Same CDS sequence)	XM_025156046.1
Accession Version	XM_025156046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGGGGT CTCCATACCA GGAGACGAGC CTGATTGGGC AGGAGACTGG 
AGCCCCGAAG GGGAAACGGG CGTCTCCTAC CCCTATTGGG AGGGTTGGCA GAACTCATTA 
AAGACTTCAA CACGGCGAAA GAAGAGGACA TCTTTTAAAA GAAAAGCCAG CAAAAGAGGC 
AATGATGATA ACAAAGGTCG GCCATTCGTG ATAAAGCCTA TTTCCTCTCC ACTCATGAAA 
CCACTGTTGG TTTTTGTAAA TCCAAAAAGT GGAGGGAACC AGGGCACCAA AGTTCTCCAA 
ATGTTTATGT GGTATTTGAA TCCACGCCAG GTCTTTGATC TCTCTCAGGA AGGGCCAAAA 
GATGCGCTGG AGCTGTACAG AAAAGTGCCA AACCTGCGGA TCCTGGCCTG TGGCGGGGAT 
GGAACGGTTG GCTGGATCCT CTCTATCCTA GATGAGCTGC AACTCACTCC TCAGCCTCCT 
GTGGCTGTCC TTCCACTTGG CACTGGGAAT GACCTTGCCC GCACCCTCAA CTGGGGTGGG 
GGTTACACAG ATGAGCCTGT GTCCAAGATC TTGTGTCATG TAGAAGATGG GACCATTGTC 
CAGCTGGACC GATGGAATCT ACAAGTTGAG CGCAACCCAG ATTTGCCACA GGATGAGCTG 
GAAGATGGGT CACGTAAGCT TCCACTCAAT GTCTTCAATA ATTACTTCAG CCTTGGGTTT 
GATGCACATG TTACACTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGCCGATTTA GAAATAAAAT GTTTTATGCA GGGGCAGCCT TTTCAGACTT CTTGCAAAGA 
AGCTCAAGAG ATTTATCCAA GCACGTTAAA GTTGTGTGTG ATGGTACAGA CCTGACTCCA 
AAGATCCAGG AGCTGAAGTT CCAGTGTATA GTGTTTTTAA ACATTCCCAG GTATTGTGCT 
GGCACAATGC CCTGGGGAAA CCCTGGAGAT CACAGAGACT TTGAACCTCA GCGCCATGAT 
GATGGCTACA TTGAAGTCAT TGGGTTCACC ATGGCCTCAC TGGCTGCTCT CCAAGTGGGT 
GGTCATGGAG AGAGGTTGCA TCAGTGCCGT GAGGTGACGC TTTTAACGTA CAAGTCTATC 
CCCATGCAAG TGGATGGTGA ACCTTGTCGG TTGGCTCCCT CACTCATTCG CATCTCCTTA 
AGGAACCAAG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CATCCATGCC TTTGTTGAAT 
GATATCCATA AAGTGCAGTC TGCTGACCTG AGGAGAGTCT CAGCTCCCCC CGATTCCTTC 
ACTGTTCCTC ATTCCATCCC TGATCGCCTG CGGATCAGAG TGAACCGGAT TAATTTGCAA 
GAATATGAGG GGCTACATTA TGACAAGGAA AAACTCCGTG AAGCTTCCAT TCCCTTAGGA 
ATTATAGTTG TACGAGGAGA TTGTGACTTG GAGACCTGTC GTATGTATAT TGACCGTCTA 
CAAGAGGATC TACAGTCAGC AACTTCTACT ACACAGAGAG TTCATTACCA GGACCAAGAA 
AGCTCTTTCC CAAGAGTACT TTCTGTTCAG AGGCTCTCTC CTAGATGGTG CTTCCTAGAT 
GCAACTTCTG CTGATCGTTT TTACCGCATT GACAGATCTC AGGAACATTT GCACTTTGTG 
ACGGAAATTT CGCAGGACGA GATTTTTATT CTGGACCCAG AGCTGGTAAT GTCACAGCAG 
GGGGGGACGC CACCTGGAAT GCCTGATCTG GTAGTGGAGC AGGCCTCTGG AATATCAGAT 
CGGTGGAACC CTGCTGTTCG AAAGAGGATG CTGAGCGACA GTGGCCTTGG CAAGATTTCC 
CCACACTACG AGGTACCTGA CAAACAAAAG GACGCCAGCC AGACCTATAT ACTGCAGTCA 
CCAGTTTCTT CAGAAGATCA AGCACTTTTA CAGGCAGTCA TAGCTGGTGA TCTGCTGAAG 
TTCATAGAAT GCTGTAAAAA AGGAGCCAAT TTGCTAATCC AAGGACCTGA CCACGGCTCC 
CTGTTGCACC ACGCTGCCAA GAGTGGGCAT GGCGAGATTG TGAAGTATAT CCTGGAACAT 
GGTCCATCTG AATTACTGGA CATGACAGAC AGTGAAACGG GTGAGACAGC ATTACATAAA 
GCGGCCTGCC AACGCCATCG TGCCATCTGC CAGCTCCTGG TGGATGCGGG AGCCTCGCTG 
AGAAAGACGG ACTCCAAGGG TAAGACTCCT CGTGACAGGG CTCAGCAAGC TGGTGATCCA 
GATCTGGCCT CATATCTGGA GAGTCGACAG AATTACCAAA TGGTTCCCCA TGAGGATTTG 
GAAACAGCAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011814.1
CDS	394..3048
Translation	MEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKGVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDIHKVQSADLRRVSAPPDSFTVPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_025156046.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156046.1

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGGGGT CTCCATACCA GGAGACGAGC CTGATTGGGC AGGAGACTGG 
AGCCCCGAAG GGGAAACGGG CGTCTCCTAC CCCTATTGGG AGGGTTGGCA GAACTCATTA 
AAGACTTCAA CACGGCGAAA GAAGAGGACA TCTTTTAAAA GAAAAGCCAG CAAAAGAGGC 
AATGATGATA ACAAAGGTCG GCCATTCGTG ATAAAGCCTA TTTCCTCTCC ACTCATGAAA 
CCACTGTTGG TTTTTGTAAA TCCAAAAAGT GGAGGGAACC AGGGCACCAA AGTTCTCCAA 
ATGTTTATGT GGTATTTGAA TCCACGCCAG GTCTTTGATC TCTCTCAGGA AGGGCCAAAA 
GATGCGCTGG AGCTGTACAG AAAAGTGCCA AACCTGCGGA TCCTGGCCTG TGGCGGGGAT 
GGAACGGTTG GCTGGATCCT CTCTATCCTA GATGAGCTGC AACTCACTCC TCAGCCTCCT 
GTGGCTGTCC TTCCACTTGG CACTGGGAAT GACCTTGCCC GCACCCTCAA CTGGGGTGGG 
GGTTACACAG ATGAGCCTGT GTCCAAGATC TTGTGTCATG TAGAAGATGG GACCATTGTC 
CAGCTGGACC GATGGAATCT ACAAGTTGAG CGCAACCCAG ATTTGCCACA GGATGAGCTG 
GAAGATGGGT CACGTAAGCT TCCACTCAAT GTCTTCAATA ATTACTTCAG CCTTGGGTTT 
GATGCACATG TTACACTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGCCGATTTA GAAATAAAAT GTTTTATGCA GGGGCAGCCT TTTCAGACTT CTTGCAAAGA 
AGCTCAAGAG ATTTATCCAA GCACGTTAAA GTTGTGTGTG ATGGTACAGA CCTGACTCCA 
AAGATCCAGG AGCTGAAGTT CCAGTGTATA GTGTTTTTAA ACATTCCCAG GTATTGTGCT 
GGCACAATGC CCTGGGGAAA CCCTGGAGAT CACAGAGACT TTGAACCTCA GCGCCATGAT 
GATGGCTACA TTGAAGTCAT TGGGTTCACC ATGGCCTCAC TGGCTGCTCT CCAAGTGGGT 
GGTCATGGAG AGAGGTTGCA TCAGTGCCGT GAGGTGACGC TTTTAACGTA CAAGTCTATC 
CCCATGCAAG TGGATGGTGA ACCTTGTCGG TTGGCTCCCT CACTCATTCG CATCTCCTTA 
AGGAACCAAG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CATCCATGCC TTTGTTGAAT 
GATATCCATA AAGTGCAGTC TGCTGACCTG AGGAGAGTCT CAGCTCCCCC CGATTCCTTC 
ACTGTTCCTC ATTCCATCCC TGATCGCCTG CGGATCAGAG TGAACCGGAT TAATTTGCAA 
GAATATGAGG GGCTACATTA TGACAAGGAA AAACTCCGTG AAGCTTCCAT TCCCTTAGGA 
ATTATAGTTG TACGAGGAGA TTGTGACTTG GAGACCTGTC GTATGTATAT TGACCGTCTA 
CAAGAGGATC TACAGTCAGC AACTTCTACT ACACAGAGAG TTCATTACCA GGACCAAGAA 
AGCTCTTTCC CAAGAGTACT TTCTGTTCAG AGGCTCTCTC CTAGATGGTG CTTCCTAGAT 
GCAACTTCTG CTGATCGTTT TTACCGCATT GACAGATCTC AGGAACATTT GCACTTTGTG 
ACGGAAATTT CGCAGGACGA GATTTTTATT CTGGACCCAG AGCTGGTAAT GTCACAGCAG 
GGGGGGACGC CACCTGGAAT GCCTGATCTG GTAGTGGAGC AGGCCTCTGG AATATCAGAT 
CGGTGGAACC CTGCTGTTCG AAAGAGGATG CTGAGCGACA GTGGCCTTGG CAAGATTTCC 
CCACACTACG AGGTACCTGA CAAACAAAAG GACGCCAGCC AGACCTATAT ACTGCAGTCA 
CCAGTTTCTT CAGAAGATCA AGCACTTTTA CAGGCAGTCA TAGCTGGTGA TCTGCTGAAG 
TTCATAGAAT GCTGTAAAAA AGGAGCCAAT TTGCTAATCC AAGGACCTGA CCACGGCTCC 
CTGTTGCACC ACGCTGCCAA GAGTGGGCAT GGCGAGATTG TGAAGTATAT CCTGGAACAT 
GGTCCATCTG AATTACTGGA CATGACAGAC AGTGAAACGG GTGAGACAGC ATTACATAAA 
GCGGCCTGCC AACGCCATCG TGCCATCTGC CAGCTCCTGG TGGATGCGGG AGCCTCGCTG 
AGAAAGACGG ACTCCAAGGG TAAGACTCCT CGTGACAGGG CTCAGCAAGC TGGTGATCCA 
GATCTGGCCT CATATCTGGA GAGTCGACAG AATTACCAAA TGGTTCCCCA TGAGGATTTG 
GAAACAGCAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_025156048.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011816.1 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa142744
Clone ID Related Accession (Same CDS sequence)	XM_025156048.1
Accession Version	XM_025156048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GGGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GGACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA TGATAACAAA GGTCGGCCAT TCGTGATAAA GCCTATTTCC 
TCTCCACTCA TGAAACCACT GTTGGTTTTT GTAAATCCAA AAAGTGGAGG GAACCAGGGC 
ACCAAAGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAAAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGTGGCG GGGATGGAAC GGTTGGCTGG ATCCTCTCTA TCCTAGATGA GCTGCAACTC 
ACTCCTCAGC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GGAATGACCT TGCCCGCACC 
CTCAACTGGG GTGGGGGTTA CACAGATGAG CCTGTGTCCA AGATCTTGTG TCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGATGG AATCTACAAG TTGAGCGCAA CCCAGATTTG 
CCACAGGATG AGCTGGAAGA TGGGTCACGT AAGCTTCCAC TCAATGTCTT CAATAATTAC 
TTCAGCCTTG GGTTTGATGC ACATGTTACA CTGGAGTTCC ACGAATCCAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCAGGGGC AGCCTTTTCA 
GACTTCTTGC AAAGAAGCTC AAGAGATTTA TCCAAGCACG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATT 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAA 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAAG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACGCTTTTA 
ACGTACAAGT CTATCCCCAT GCAAGTGGAT GGTGAACCTT GTCGGTTGGC TCCCTCACTC 
ATTCGCATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTGT TGAATGATCC TCATTCCATC CCTGATCGCC TGCGGATCAG AGTGAACCGG 
ATTAATTTGC AAGAATATGA GGGGCTACAT TATGACAAGG AAAAACTCCG TGAAGCTTCC 
ATTCCCTTAG GAATTATAGT TGTACGAGGA GATTGTGACT TGGAGACCTG TCGTATGTAT 
ATTGACCGTC TACAAGAGGA TCTACAGTCA GCAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAAG AAAGCTCTTT CCCAAGAGTA CTTTCTGTTC AGAGGCTCTC TCCTAGATGG 
TGCTTCCTAG ATGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTCTGGACCC AGAGCTGGTA 
ATGTCACAGC AGGGGGGGAC GCCACCTGGA ATGCCTGATC TGGTAGTGGA GCAGGCCTCT 
GGAATATCAG ATCGGTGGAA CCCTGCTGTT CGAAAGAGGA TGCTGAGCGA CAGTGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACCTAT 
ATACTGCAGT CACCAGTTTC TTCAGAAGAT CAAGCACTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAAGGAGCCA ATTTGCTAAT CCAAGGACCT 
GACCACGGCT CCCTGTTGCA CCACGCTGCC AAGAGTGGGC ATGGCGAGAT TGTGAAGTAT 
ATCCTGGAAC ATGGTCCATC TGAATTACTG GACATGACAG ACAGTGAAAC GGGTGAGACA 
GCATTACATA AAGCGGCCTG CCAACGCCAT CGTGCCATCT GCCAGCTCCT GGTGGATGCG 
GGAGCCTCGC TGAGAAAGAC GGACTCCAAG GGTAAGACTC CTCGTGACAG GGCTCAGCAA 
GCTGGTGATC CAGATCTGGC CTCATATCTG GAGAGTCGAC AGAATTACCA AATGGTTCCC 
CATGAGGATT TGGAAACAGC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011816.1
CDS	391..2997
Translation	MEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQGVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_025156048.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156048.1

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG GGGGTCTCCA TACCAGGAGA CGAGCCTGAT 
TGGGCAGGAG ACTGGAGCCC CGAAGGGGAA ACGGGCGTCT CCTACCCCTA TTGGGAGGGT 
TGGCAGAACT CATTAAAGAC TTCAACACGG CGAAAGAAGA GGACATCTTT TAAAAGAAAA 
GCCAGCAAAA GAGGCAATGA TGATAACAAA GGTCGGCCAT TCGTGATAAA GCCTATTTCC 
TCTCCACTCA TGAAACCACT GTTGGTTTTT GTAAATCCAA AAAGTGGAGG GAACCAGGGC 
ACCAAAGTTC TCCAAATGTT TATGTGGTAT TTGAATCCAC GCCAGGTCTT TGATCTCTCT 
CAGGAAGGGC CAAAAGATGC GCTGGAGCTG TACAGAAAAG TGCCAAACCT GCGGATCCTG 
GCCTGTGGCG GGGATGGAAC GGTTGGCTGG ATCCTCTCTA TCCTAGATGA GCTGCAACTC 
ACTCCTCAGC CTCCTGTGGC TGTCCTTCCA CTTGGCACTG GGAATGACCT TGCCCGCACC 
CTCAACTGGG GTGGGGGTTA CACAGATGAG CCTGTGTCCA AGATCTTGTG TCATGTAGAA 
GATGGGACCA TTGTCCAGCT GGACCGATGG AATCTACAAG TTGAGCGCAA CCCAGATTTG 
CCACAGGATG AGCTGGAAGA TGGGTCACGT AAGCTTCCAC TCAATGTCTT CAATAATTAC 
TTCAGCCTTG GGTTTGATGC ACATGTTACA CTGGAGTTCC ACGAATCCAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGCCG ATTTAGAAAT AAAATGTTTT ATGCAGGGGC AGCCTTTTCA 
GACTTCTTGC AAAGAAGCTC AAGAGATTTA TCCAAGCACG TTAAAGTTGT GTGTGATGGT 
ACAGACCTGA CTCCAAAGAT CCAGGAGCTG AAGTTCCAGT GTATAGTGTT TTTAAACATT 
CCCAGGTATT GTGCTGGCAC AATGCCCTGG GGAAACCCTG GAGATCACAG AGACTTTGAA 
CCTCAGCGCC ATGATGATGG CTACATTGAA GTCATTGGGT TCACCATGGC CTCACTGGCT 
GCTCTCCAAG TGGGTGGTCA TGGAGAGAGG TTGCATCAGT GCCGTGAGGT GACGCTTTTA 
ACGTACAAGT CTATCCCCAT GCAAGTGGAT GGTGAACCTT GTCGGTTGGC TCCCTCACTC 
ATTCGCATCT CCTTAAGGAA CCAAGCCAAC ATGGTGCAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTGT TGAATGATCC TCATTCCATC CCTGATCGCC TGCGGATCAG AGTGAACCGG 
ATTAATTTGC AAGAATATGA GGGGCTACAT TATGACAAGG AAAAACTCCG TGAAGCTTCC 
ATTCCCTTAG GAATTATAGT TGTACGAGGA GATTGTGACT TGGAGACCTG TCGTATGTAT 
ATTGACCGTC TACAAGAGGA TCTACAGTCA GCAACTTCTA CTACACAGAG AGTTCATTAC 
CAGGACCAAG AAAGCTCTTT CCCAAGAGTA CTTTCTGTTC AGAGGCTCTC TCCTAGATGG 
TGCTTCCTAG ATGCAACTTC TGCTGATCGT TTTTACCGCA TTGACAGATC TCAGGAACAT 
TTGCACTTTG TGACGGAAAT TTCGCAGGAC GAGATTTTTA TTCTGGACCC AGAGCTGGTA 
ATGTCACAGC AGGGGGGGAC GCCACCTGGA ATGCCTGATC TGGTAGTGGA GCAGGCCTCT 
GGAATATCAG ATCGGTGGAA CCCTGCTGTT CGAAAGAGGA TGCTGAGCGA CAGTGGCCTT 
GGCAAGATTT CCCCACACTA CGAGGTACCT GACAAACAAA AGGACGCCAG CCAGACCTAT 
ATACTGCAGT CACCAGTTTC TTCAGAAGAT CAAGCACTTT TACAGGCAGT CATAGCTGGT 
GATCTGCTGA AGTTCATAGA ATGCTGTAAA AAAGGAGCCA ATTTGCTAAT CCAAGGACCT 
GACCACGGCT CCCTGTTGCA CCACGCTGCC AAGAGTGGGC ATGGCGAGAT TGTGAAGTAT 
ATCCTGGAAC ATGGTCCATC TGAATTACTG GACATGACAG ACAGTGAAAC GGGTGAGACA 
GCATTACATA AAGCGGCCTG CCAACGCCAT CGTGCCATCT GCCAGCTCCT GGTGGATGCG 
GGAGCCTCGC TGAGAAAGAC GGACTCCAAG GGTAAGACTC CTCGTGACAG GGCTCAGCAA 
GCTGGTGATC CAGATCTGGC CTCATATCTG GAGAGTCGAC AGAATTACCA AATGGTTCCC 
CATGAGGATT TGGAAACAGC AGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_025156051.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011819.1 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OGa142745
Clone ID Related Accession (Same CDS sequence)	XM_025156051.1
Accession Version	XM_025156051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2574bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG AACTCATTAA AGACTTCAAC ACGGCGAAAG 
AAGAGGACAT CTTTTAAAAG AAAAGCCAGC AAAAGAGGCA ATGATGATAA CAAAGGTCGG 
CCATTCGTGA TAAAGCCTAT TTCCTCTCCA CTCATGAAAC CACTGTTGGT TTTTGTAAAT 
CCAAAAAGTG GAGGGAACCA GGGCACCAAA GTTCTCCAAA TGTTTATGTG GTATTTGAAT 
CCACGCCAGG TCTTTGATCT CTCTCAGGAA GGGCCAAAAG ATGCGCTGGA GCTGTACAGA 
AAAGTGCCAA ACCTGCGGAT CCTGGCCTGT GGCGGGGATG GAACGGTTGG CTGGATCCTC 
TCTATCCTAG ATGAGCTGCA ACTCACTCCT CAGCCTCCTG TGGCTGTCCT TCCACTTGGC 
ACTGGGAATG ACCTTGCCCG CACCCTCAAC TGGGGTGGGG GTTACACAGA TGAGCCTGTG 
TCCAAGATCT TGTGTCATGT AGAAGATGGG ACCATTGTCC AGCTGGACCG ATGGAATCTA 
CAAGTTGAGC GCAACCCAGA TTTGCCACAG GATGAGCTGG AAGATGGGTC ACGTAAGCTT 
CCACTCAATG TCTTCAATAA TTACTTCAGC CTTGGGTTTG ATGCACATGT TACACTGGAG 
TTCCACGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GCCGATTTAG AAATAAAATG 
TTTTATGCAG GGGCAGCCTT TTCAGACTTC TTGCAAAGAA GCTCAAGAGA TTTATCCAAG 
CACGTTAAAG TTGTGTGTGA TGGTACAGAC CTGACTCCAA AGATCCAGGA GCTGAAGTTC 
CAGTGTATAG TGTTTTTAAA CATTCCCAGG TATTGTGCTG GCACAATGCC CTGGGGAAAC 
CCTGGAGATC ACAGAGACTT TGAACCTCAG CGCCATGATG ATGGCTACAT TGAAGTCATT 
GGGTTCACCA TGGCCTCACT GGCTGCTCTC CAAGTGGGTG GTCATGGAGA GAGGTTGCAT 
CAGTGCCGTG AGGTGACGCT TTTAACGTAC AAGTCTATCC CCATGCAAGT GGATGGTGAA 
CCTTGTCGGT TGGCTCCCTC ACTCATTCGC ATCTCCTTAA GGAACCAAGC CAACATGGTG 
CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTGTTGAATG ATATCCATAA AGTGCAGTCT 
GCTGACCTGA GGAGAGTCTC AGCTCCCCCC GATTCCTTCA CTGTTCCTCA TTCCATCCCT 
GATCGCCTGC GGATCAGAGT GAACCGGATT AATTTGCAAG AATATGAGGG GCTACATTAT 
GACAAGGAAA AACTCCGTGA AGCTTCCATT CCCTTAGGAA TTATAGTTGT ACGAGGAGAT 
TGTGACTTGG AGACCTGTCG TATGTATATT GACCGTCTAC AAGAGGATCT ACAGTCAGCA 
ACTTCTACTA CACAGAGAGT TCATTACCAG GACCAAGAAA GCTCTTTCCC AAGAGTACTT 
TCTGTTCAGA GGCTCTCTCC TAGATGGTGC TTCCTAGATG CAACTTCTGC TGATCGTTTT 
TACCGCATTG ACAGATCTCA GGAACATTTG CACTTTGTGA CGGAAATTTC GCAGGACGAG 
ATTTTTATTC TGGACCCAGA GCTGGTAATG TCACAGCAGG GGGGGACGCC ACCTGGAATG 
CCTGATCTGG TAGTGGAGCA GGCCTCTGGA ATATCAGATC GGTGGAACCC TGCTGTTCGA 
AAGAGGATGC TGAGCGACAG TGGCCTTGGC AAGATTTCCC CACACTACGA GGTACCTGAC 
AAACAAAAGG ACGCCAGCCA GACCTATATA CTGCAGTCAC CAGTTTCTTC AGAAGATCAA 
GCACTTTTAC AGGCAGTCAT AGCTGGTGAT CTGCTGAAGT TCATAGAATG CTGTAAAAAA 
GGAGCCAATT TGCTAATCCA AGGACCTGAC CACGGCTCCC TGTTGCACCA CGCTGCCAAG 
AGTGGGCATG GCGAGATTGT GAAGTATATC CTGGAACATG GTCCATCTGA ATTACTGGAC 
ATGACAGACA GTGAAACGGG TGAGACAGCA TTACATAAAG CGGCCTGCCA ACGCCATCGT 
GCCATCTGCC AGCTCCTGGT GGATGCGGGA GCCTCGCTGA GAAAGACGGA CTCCAAGGGT 
AAGACTCCTC GTGACAGGGC TCAGCAAGCT GGTGATCCAG ATCTGGCCTC ATATCTGGAG 
AGTCGACAGA ATTACCAAAT GGTTCCCCAT GAGGATTTGG AAACAGCAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011819.1
CDS	393..2966
Translation	MEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDIHKVQSADLRRVSAPPDSFTVPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_025156051.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156051.1

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG AACTCATTAA AGACTTCAAC ACGGCGAAAG 
AAGAGGACAT CTTTTAAAAG AAAAGCCAGC AAAAGAGGCA ATGATGATAA CAAAGGTCGG 
CCATTCGTGA TAAAGCCTAT TTCCTCTCCA CTCATGAAAC CACTGTTGGT TTTTGTAAAT 
CCAAAAAGTG GAGGGAACCA GGGCACCAAA GTTCTCCAAA TGTTTATGTG GTATTTGAAT 
CCACGCCAGG TCTTTGATCT CTCTCAGGAA GGGCCAAAAG ATGCGCTGGA GCTGTACAGA 
AAAGTGCCAA ACCTGCGGAT CCTGGCCTGT GGCGGGGATG GAACGGTTGG CTGGATCCTC 
TCTATCCTAG ATGAGCTGCA ACTCACTCCT CAGCCTCCTG TGGCTGTCCT TCCACTTGGC 
ACTGGGAATG ACCTTGCCCG CACCCTCAAC TGGGGTGGGG GTTACACAGA TGAGCCTGTG 
TCCAAGATCT TGTGTCATGT AGAAGATGGG ACCATTGTCC AGCTGGACCG ATGGAATCTA 
CAAGTTGAGC GCAACCCAGA TTTGCCACAG GATGAGCTGG AAGATGGGTC ACGTAAGCTT 
CCACTCAATG TCTTCAATAA TTACTTCAGC CTTGGGTTTG ATGCACATGT TACACTGGAG 
TTCCACGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GCCGATTTAG AAATAAAATG 
TTTTATGCAG GGGCAGCCTT TTCAGACTTC TTGCAAAGAA GCTCAAGAGA TTTATCCAAG 
CACGTTAAAG TTGTGTGTGA TGGTACAGAC CTGACTCCAA AGATCCAGGA GCTGAAGTTC 
CAGTGTATAG TGTTTTTAAA CATTCCCAGG TATTGTGCTG GCACAATGCC CTGGGGAAAC 
CCTGGAGATC ACAGAGACTT TGAACCTCAG CGCCATGATG ATGGCTACAT TGAAGTCATT 
GGGTTCACCA TGGCCTCACT GGCTGCTCTC CAAGTGGGTG GTCATGGAGA GAGGTTGCAT 
CAGTGCCGTG AGGTGACGCT TTTAACGTAC AAGTCTATCC CCATGCAAGT GGATGGTGAA 
CCTTGTCGGT TGGCTCCCTC ACTCATTCGC ATCTCCTTAA GGAACCAAGC CAACATGGTG 
CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTGTTGAATG ATATCCATAA AGTGCAGTCT 
GCTGACCTGA GGAGAGTCTC AGCTCCCCCC GATTCCTTCA CTGTTCCTCA TTCCATCCCT 
GATCGCCTGC GGATCAGAGT GAACCGGATT AATTTGCAAG AATATGAGGG GCTACATTAT 
GACAAGGAAA AACTCCGTGA AGCTTCCATT CCCTTAGGAA TTATAGTTGT ACGAGGAGAT 
TGTGACTTGG AGACCTGTCG TATGTATATT GACCGTCTAC AAGAGGATCT ACAGTCAGCA 
ACTTCTACTA CACAGAGAGT TCATTACCAG GACCAAGAAA GCTCTTTCCC AAGAGTACTT 
TCTGTTCAGA GGCTCTCTCC TAGATGGTGC TTCCTAGATG CAACTTCTGC TGATCGTTTT 
TACCGCATTG ACAGATCTCA GGAACATTTG CACTTTGTGA CGGAAATTTC GCAGGACGAG 
ATTTTTATTC TGGACCCAGA GCTGGTAATG TCACAGCAGG GGGGGACGCC ACCTGGAATG 
CCTGATCTGG TAGTGGAGCA GGCCTCTGGA ATATCAGATC GGTGGAACCC TGCTGTTCGA 
AAGAGGATGC TGAGCGACAG TGGCCTTGGC AAGATTTCCC CACACTACGA GGTACCTGAC 
AAACAAAAGG ACGCCAGCCA GACCTATATA CTGCAGTCAC CAGTTTCTTC AGAAGATCAA 
GCACTTTTAC AGGCAGTCAT AGCTGGTGAT CTGCTGAAGT TCATAGAATG CTGTAAAAAA 
GGAGCCAATT TGCTAATCCA AGGACCTGAC CACGGCTCCC TGTTGCACCA CGCTGCCAAG 
AGTGGGCATG GCGAGATTGT GAAGTATATC CTGGAACATG GTCCATCTGA ATTACTGGAC 
ATGACAGACA GTGAAACGGG TGAGACAGCA TTACATAAAG CGGCCTGCCA ACGCCATCGT 
GCCATCTGCC AGCTCCTGGT GGATGCGGGA GCCTCGCTGA GAAAGACGGA CTCCAAGGGT 
AAGACTCCTC GTGACAGGGC TCAGCAAGCT GGTGATCCAG ATCTGGCCTC ATATCTGGAG 
AGTCGACAGA ATTACCAAAT GGTTCCCCAT GAGGATTTGG AAACAGCAGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_025156054.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011822.1 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OGa142746
Clone ID Related Accession (Same CDS sequence)	XM_025156054.1
Accession Version	XM_025156054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGAACTC ATTAAAGACT TCAACACGGC GAAAGAAGAG GACATCTTTT 
AAAAGAAAAG CCAGCAAAAG AGGCAATGAT GATAACAAAG GTCGGCCATT CGTGATAAAG 
CCTATTTCCT CTCCACTCAT GAAACCACTG TTGGTTTTTG TAAATCCAAA AAGTGGAGGG 
AACCAGGGCA CCAAAGTTCT CCAAATGTTT ATGTGGTATT TGAATCCACG CCAGGTCTTT 
GATCTCTCTC AGGAAGGGCC AAAAGATGCG CTGGAGCTGT ACAGAAAAGT GCCAAACCTG 
CGGATCCTGG CCTGTGGCGG GGATGGAACG GTTGGCTGGA TCCTCTCTAT CCTAGATGAG 
CTGCAACTCA CTCCTCAGCC TCCTGTGGCT GTCCTTCCAC TTGGCACTGG GAATGACCTT 
GCCCGCACCC TCAACTGGGG TGGGGGTTAC ACAGATGAGC CTGTGTCCAA GATCTTGTGT 
CATGTAGAAG ATGGGACCAT TGTCCAGCTG GACCGATGGA ATCTACAAGT TGAGCGCAAC 
CCAGATTTGC CACAGGATGA GCTGGAAGAT GGGTCACGTA AGCTTCCACT CAATGTCTTC 
AATAATTACT TCAGCCTTGG GTTTGATGCA CATGTTACAC TGGAGTTCCA CGAATCCAGA 
GAAGCAAATC CAGAGAAATT CAACAGCCGA TTTAGAAATA AAATGTTTTA TGCAGGGGCA 
GCCTTTTCAG ACTTCTTGCA AAGAAGCTCA AGAGATTTAT CCAAGCACGT TAAAGTTGTG 
TGTGATGGTA CAGACCTGAC TCCAAAGATC CAGGAGCTGA AGTTCCAGTG TATAGTGTTT 
TTAAACATTC CCAGGTATTG TGCTGGCACA ATGCCCTGGG GAAACCCTGG AGATCACAGA 
GACTTTGAAC CTCAGCGCCA TGATGATGGC TACATTGAAG TCATTGGGTT CACCATGGCC 
TCACTGGCTG CTCTCCAAGT GGGTGGTCAT GGAGAGAGGT TGCATCAGTG CCGTGAGGTG 
ACGCTTTTAA CGTACAAGTC TATCCCCATG CAAGTGGATG GTGAACCTTG TCGGTTGGCT 
CCCTCACTCA TTCGCATCTC CTTAAGGAAC CAAGCCAACA TGGTGCAGAA GAGCAAGAGG 
AGAACATCCA TGCCTTTGTT GAATGATATC CATAAAGTGC AGTCTGCTGA CCTGAGGAGA 
GTCTCAGCTC CCCCCGATTC CTTCACTGTT CCTCATTCCA TCCCTGATCG CCTGCGGATC 
AGAGTGAACC GGATTAATTT GCAAGAATAT GAGGGGCTAC ATTATGACAA GGAAAAACTC 
CGTGAAGCTT CCATTCCCTT AGGAATTATA GTTGTACGAG GAGATTGTGA CTTGGAGACC 
TGTCGTATGT ATATTGACCG TCTACAAGAG GATCTACAGT CAGCAACTTC TACTACACAG 
AGAGTTCATT ACCAGGACCA AGAAAGCTCT TTCCCAAGAG TACTTTCTGT TCAGAGGCTC 
TCTCCTAGAT GGTGCTTCCT AGATGCAACT TCTGCTGATC GTTTTTACCG CATTGACAGA 
TCTCAGGAAC ATTTGCACTT TGTGACGGAA ATTTCGCAGG ACGAGATTTT TATTCTGGAC 
CCAGAGCTGG TAATGTCACA GCAGGGGGGG ACGCCACCTG GAATGCCTGA TCTGGTAGTG 
GAGCAGGCCT CTGGAATATC AGATCGGTGG AACCCTGCTG TTCGAAAGAG GATGCTGAGC 
GACAGTGGCC TTGGCAAGAT TTCCCCACAC TACGAGGTAC CTGACAAACA AAAGGACGCC 
AGCCAGACCT ATATACTGCA GTCACCAGTT TCTTCAGAAG ATCAAGCACT TTTACAGGCA 
GTCATAGCTG GTGATCTGCT GAAGTTCATA GAATGCTGTA AAAAAGGAGC CAATTTGCTA 
ATCCAAGGAC CTGACCACGG CTCCCTGTTG CACCACGCTG CCAAGAGTGG GCATGGCGAG 
ATTGTGAAGT ATATCCTGGA ACATGGTCCA TCTGAATTAC TGGACATGAC AGACAGTGAA 
ACGGGTGAGA CAGCATTACA TAAAGCGGCC TGCCAACGCC ATCGTGCCAT CTGCCAGCTC 
CTGGTGGATG CGGGAGCCTC GCTGAGAAAG ACGGACTCCA AGGGTAAGAC TCCTCGTGAC 
AGGGCTCAGC AAGCTGGTGA TCCAGATCTG GCCTCATATC TGGAGAGTCG ACAGAATTAC 
CAAATGGTTC CCCATGAGGA TTTGGAAACA GCAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011822.1
CDS	393..2951
Translation	MEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDIHKVQSADLRRVSAPPDSFTVPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_025156054.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156054.1

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGAACTC ATTAAAGACT TCAACACGGC GAAAGAAGAG GACATCTTTT 
AAAAGAAAAG CCAGCAAAAG AGGCAATGAT GATAACAAAG GTCGGCCATT CGTGATAAAG 
CCTATTTCCT CTCCACTCAT GAAACCACTG TTGGTTTTTG TAAATCCAAA AAGTGGAGGG 
AACCAGGGCA CCAAAGTTCT CCAAATGTTT ATGTGGTATT TGAATCCACG CCAGGTCTTT 
GATCTCTCTC AGGAAGGGCC AAAAGATGCG CTGGAGCTGT ACAGAAAAGT GCCAAACCTG 
CGGATCCTGG CCTGTGGCGG GGATGGAACG GTTGGCTGGA TCCTCTCTAT CCTAGATGAG 
CTGCAACTCA CTCCTCAGCC TCCTGTGGCT GTCCTTCCAC TTGGCACTGG GAATGACCTT 
GCCCGCACCC TCAACTGGGG TGGGGGTTAC ACAGATGAGC CTGTGTCCAA GATCTTGTGT 
CATGTAGAAG ATGGGACCAT TGTCCAGCTG GACCGATGGA ATCTACAAGT TGAGCGCAAC 
CCAGATTTGC CACAGGATGA GCTGGAAGAT GGGTCACGTA AGCTTCCACT CAATGTCTTC 
AATAATTACT TCAGCCTTGG GTTTGATGCA CATGTTACAC TGGAGTTCCA CGAATCCAGA 
GAAGCAAATC CAGAGAAATT CAACAGCCGA TTTAGAAATA AAATGTTTTA TGCAGGGGCA 
GCCTTTTCAG ACTTCTTGCA AAGAAGCTCA AGAGATTTAT CCAAGCACGT TAAAGTTGTG 
TGTGATGGTA CAGACCTGAC TCCAAAGATC CAGGAGCTGA AGTTCCAGTG TATAGTGTTT 
TTAAACATTC CCAGGTATTG TGCTGGCACA ATGCCCTGGG GAAACCCTGG AGATCACAGA 
GACTTTGAAC CTCAGCGCCA TGATGATGGC TACATTGAAG TCATTGGGTT CACCATGGCC 
TCACTGGCTG CTCTCCAAGT GGGTGGTCAT GGAGAGAGGT TGCATCAGTG CCGTGAGGTG 
ACGCTTTTAA CGTACAAGTC TATCCCCATG CAAGTGGATG GTGAACCTTG TCGGTTGGCT 
CCCTCACTCA TTCGCATCTC CTTAAGGAAC CAAGCCAACA TGGTGCAGAA GAGCAAGAGG 
AGAACATCCA TGCCTTTGTT GAATGATATC CATAAAGTGC AGTCTGCTGA CCTGAGGAGA 
GTCTCAGCTC CCCCCGATTC CTTCACTGTT CCTCATTCCA TCCCTGATCG CCTGCGGATC 
AGAGTGAACC GGATTAATTT GCAAGAATAT GAGGGGCTAC ATTATGACAA GGAAAAACTC 
CGTGAAGCTT CCATTCCCTT AGGAATTATA GTTGTACGAG GAGATTGTGA CTTGGAGACC 
TGTCGTATGT ATATTGACCG TCTACAAGAG GATCTACAGT CAGCAACTTC TACTACACAG 
AGAGTTCATT ACCAGGACCA AGAAAGCTCT TTCCCAAGAG TACTTTCTGT TCAGAGGCTC 
TCTCCTAGAT GGTGCTTCCT AGATGCAACT TCTGCTGATC GTTTTTACCG CATTGACAGA 
TCTCAGGAAC ATTTGCACTT TGTGACGGAA ATTTCGCAGG ACGAGATTTT TATTCTGGAC 
CCAGAGCTGG TAATGTCACA GCAGGGGGGG ACGCCACCTG GAATGCCTGA TCTGGTAGTG 
GAGCAGGCCT CTGGAATATC AGATCGGTGG AACCCTGCTG TTCGAAAGAG GATGCTGAGC 
GACAGTGGCC TTGGCAAGAT TTCCCCACAC TACGAGGTAC CTGACAAACA AAAGGACGCC 
AGCCAGACCT ATATACTGCA GTCACCAGTT TCTTCAGAAG ATCAAGCACT TTTACAGGCA 
GTCATAGCTG GTGATCTGCT GAAGTTCATA GAATGCTGTA AAAAAGGAGC CAATTTGCTA 
ATCCAAGGAC CTGACCACGG CTCCCTGTTG CACCACGCTG CCAAGAGTGG GCATGGCGAG 
ATTGTGAAGT ATATCCTGGA ACATGGTCCA TCTGAATTAC TGGACATGAC AGACAGTGAA 
ACGGGTGAGA CAGCATTACA TAAAGCGGCC TGCCAACGCC ATCGTGCCAT CTGCCAGCTC 
CTGGTGGATG CGGGAGCCTC GCTGAGAAAG ACGGACTCCA AGGGTAAGAC TCCTCGTGAC 
AGGGCTCAGC AAGCTGGTGA TCCAGATCTG GCCTCATATC TGGAGAGTCG ACAGAATTAC 
CAAATGGTTC CCCATGAGGA TTTGGAAACA GCAGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_025156057.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011825.1 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OGa142747
Clone ID Related Accession (Same CDS sequence)	XM_025156057.1
Accession Version	XM_025156057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG AACTCATTAA AGACTTCAAC ACGGCGAAAG 
AAGAGGACAT CTTTTAAAAG AAAAGCCAGC AAAAGAGGCA ATGATGATAA CAAAGGTCGG 
CCATTCGTGA TAAAGCCTAT TTCCTCTCCA CTCATGAAAC CACTGTTGGT TTTTGTAAAT 
CCAAAAAGTG GAGGGAACCA GGGCACCAAA GTTCTCCAAA TGTTTATGTG GTATTTGAAT 
CCACGCCAGG TCTTTGATCT CTCTCAGGAA GGGCCAAAAG ATGCGCTGGA GCTGTACAGA 
AAAGTGCCAA ACCTGCGGAT CCTGGCCTGT GGCGGGGATG GAACGGTTGG CTGGATCCTC 
TCTATCCTAG ATGAGCTGCA ACTCACTCCT CAGCCTCCTG TGGCTGTCCT TCCACTTGGC 
ACTGGGAATG ACCTTGCCCG CACCCTCAAC TGGGGTGGGG GTTACACAGA TGAGCCTGTG 
TCCAAGATCT TGTGTCATGT AGAAGATGGG ACCATTGTCC AGCTGGACCG ATGGAATCTA 
CAAGTTGAGC GCAACCCAGA TTTGCCACAG GATGAGCTGG AAGATGGGTC ACGTAAGCTT 
CCACTCAATG TCTTCAATAA TTACTTCAGC CTTGGGTTTG ATGCACATGT TACACTGGAG 
TTCCACGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GCCGATTTAG AAATAAAATG 
TTTTATGCAG GGGCAGCCTT TTCAGACTTC TTGCAAAGAA GCTCAAGAGA TTTATCCAAG 
CACGTTAAAG TTGTGTGTGA TGGTACAGAC CTGACTCCAA AGATCCAGGA GCTGAAGTTC 
CAGTGTATAG TGTTTTTAAA CATTCCCAGG TATTGTGCTG GCACAATGCC CTGGGGAAAC 
CCTGGAGATC ACAGAGACTT TGAACCTCAG CGCCATGATG ATGGCTACAT TGAAGTCATT 
GGGTTCACCA TGGCCTCACT GGCTGCTCTC CAAGTGGGTG GTCATGGAGA GAGGTTGCAT 
CAGTGCCGTG AGGTGACGCT TTTAACGTAC AAGTCTATCC CCATGCAAGT GGATGGTGAA 
CCTTGTCGGT TGGCTCCCTC ACTCATTCGC ATCTCCTTAA GGAACCAAGC CAACATGGTG 
CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTGTTGAATG ATCCTCATTC CATCCCTGAT 
CGCCTGCGGA TCAGAGTGAA CCGGATTAAT TTGCAAGAAT ATGAGGGGCT ACATTATGAC 
AAGGAAAAAC TCCGTGAAGC TTCCATTCCC TTAGGAATTA TAGTTGTACG AGGAGATTGT 
GACTTGGAGA CCTGTCGTAT GTATATTGAC CGTCTACAAG AGGATCTACA GTCAGCAACT 
TCTACTACAC AGAGAGTTCA TTACCAGGAC CAAGAAAGCT CTTTCCCAAG AGTACTTTCT 
GTTCAGAGGC TCTCTCCTAG ATGGTGCTTC CTAGATGCAA CTTCTGCTGA TCGTTTTTAC 
CGCATTGACA GATCTCAGGA ACATTTGCAC TTTGTGACGG AAATTTCGCA GGACGAGATT 
TTTATTCTGG ACCCAGAGCT GGTAATGTCA CAGCAGGGGG GGACGCCACC TGGAATGCCT 
GATCTGGTAG TGGAGCAGGC CTCTGGAATA TCAGATCGGT GGAACCCTGC TGTTCGAAAG 
AGGATGCTGA GCGACAGTGG CCTTGGCAAG ATTTCCCCAC ACTACGAGGT ACCTGACAAA 
CAAAAGGACG CCAGCCAGAC CTATATACTG CAGTCACCAG TTTCTTCAGA AGATCAAGCA 
CTTTTACAGG CAGTCATAGC TGGTGATCTG CTGAAGTTCA TAGAATGCTG TAAAAAAGGA 
GCCAATTTGC TAATCCAAGG ACCTGACCAC GGCTCCCTGT TGCACCACGC TGCCAAGAGT 
GGGCATGGCG AGATTGTGAA GTATATCCTG GAACATGGTC CATCTGAATT ACTGGACATG 
ACAGACAGTG AAACGGGTGA GACAGCATTA CATAAAGCGG CCTGCCAACG CCATCGTGCC 
ATCTGCCAGC TCCTGGTGGA TGCGGGAGCC TCGCTGAGAA AGACGGACTC CAAGGGTAAG 
ACTCCTCGTG ACAGGGCTCA GCAAGCTGGT GATCCAGATC TGGCCTCATA TCTGGAGAGT 
CGACAGAATT ACCAAATGGT TCCCCATGAG GATTTGGAAA CAGCAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011825.1
CDS	390..2900
Translation	MEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_025156057.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156057.1

ORF Insert Sequence:

ATGGAGCAGT TAGAGAAGAT TAATTTTCGC TGCAAACCAA CTTTCCGAGA AGGGAGCTCC 
AGGTCTCCAA GAGAAAACTT TGTCCGACAT CACTGGGTAC ACAGACGGAG GCAGGAGGGG 
AAATGTAAGC AGTGTGGAAA GGGCTTTCAG CAGAAATTCT CCTTCCATAG TAAAGAGATT 
GTGGCCATCA GTTGTTCCTG GTGCAAGCTG GCGTTTCATA ACAAAGTCAC CTGCTTCATG 
CTACATCACA TTGAGGAGCC ATGTTCTCTG GGGGCTCACG CGGCTGTCAT TGTGCCTCCC 
ACCTGGATCA TTAAGGTGAA GAAACCTCAG AACTCATTAA AGACTTCAAC ACGGCGAAAG 
AAGAGGACAT CTTTTAAAAG AAAAGCCAGC AAAAGAGGCA ATGATGATAA CAAAGGTCGG 
CCATTCGTGA TAAAGCCTAT TTCCTCTCCA CTCATGAAAC CACTGTTGGT TTTTGTAAAT 
CCAAAAAGTG GAGGGAACCA GGGCACCAAA GTTCTCCAAA TGTTTATGTG GTATTTGAAT 
CCACGCCAGG TCTTTGATCT CTCTCAGGAA GGGCCAAAAG ATGCGCTGGA GCTGTACAGA 
AAAGTGCCAA ACCTGCGGAT CCTGGCCTGT GGCGGGGATG GAACGGTTGG CTGGATCCTC 
TCTATCCTAG ATGAGCTGCA ACTCACTCCT CAGCCTCCTG TGGCTGTCCT TCCACTTGGC 
ACTGGGAATG ACCTTGCCCG CACCCTCAAC TGGGGTGGGG GTTACACAGA TGAGCCTGTG 
TCCAAGATCT TGTGTCATGT AGAAGATGGG ACCATTGTCC AGCTGGACCG ATGGAATCTA 
CAAGTTGAGC GCAACCCAGA TTTGCCACAG GATGAGCTGG AAGATGGGTC ACGTAAGCTT 
CCACTCAATG TCTTCAATAA TTACTTCAGC CTTGGGTTTG ATGCACATGT TACACTGGAG 
TTCCACGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GCCGATTTAG AAATAAAATG 
TTTTATGCAG GGGCAGCCTT TTCAGACTTC TTGCAAAGAA GCTCAAGAGA TTTATCCAAG 
CACGTTAAAG TTGTGTGTGA TGGTACAGAC CTGACTCCAA AGATCCAGGA GCTGAAGTTC 
CAGTGTATAG TGTTTTTAAA CATTCCCAGG TATTGTGCTG GCACAATGCC CTGGGGAAAC 
CCTGGAGATC ACAGAGACTT TGAACCTCAG CGCCATGATG ATGGCTACAT TGAAGTCATT 
GGGTTCACCA TGGCCTCACT GGCTGCTCTC CAAGTGGGTG GTCATGGAGA GAGGTTGCAT 
CAGTGCCGTG AGGTGACGCT TTTAACGTAC AAGTCTATCC CCATGCAAGT GGATGGTGAA 
CCTTGTCGGT TGGCTCCCTC ACTCATTCGC ATCTCCTTAA GGAACCAAGC CAACATGGTG 
CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTGTTGAATG ATCCTCATTC CATCCCTGAT 
CGCCTGCGGA TCAGAGTGAA CCGGATTAAT TTGCAAGAAT ATGAGGGGCT ACATTATGAC 
AAGGAAAAAC TCCGTGAAGC TTCCATTCCC TTAGGAATTA TAGTTGTACG AGGAGATTGT 
GACTTGGAGA CCTGTCGTAT GTATATTGAC CGTCTACAAG AGGATCTACA GTCAGCAACT 
TCTACTACAC AGAGAGTTCA TTACCAGGAC CAAGAAAGCT CTTTCCCAAG AGTACTTTCT 
GTTCAGAGGC TCTCTCCTAG ATGGTGCTTC CTAGATGCAA CTTCTGCTGA TCGTTTTTAC 
CGCATTGACA GATCTCAGGA ACATTTGCAC TTTGTGACGG AAATTTCGCA GGACGAGATT 
TTTATTCTGG ACCCAGAGCT GGTAATGTCA CAGCAGGGGG GGACGCCACC TGGAATGCCT 
GATCTGGTAG TGGAGCAGGC CTCTGGAATA TCAGATCGGT GGAACCCTGC TGTTCGAAAG 
AGGATGCTGA GCGACAGTGG CCTTGGCAAG ATTTCCCCAC ACTACGAGGT ACCTGACAAA 
CAAAAGGACG CCAGCCAGAC CTATATACTG CAGTCACCAG TTTCTTCAGA AGATCAAGCA 
CTTTTACAGG CAGTCATAGC TGGTGATCTG CTGAAGTTCA TAGAATGCTG TAAAAAAGGA 
GCCAATTTGC TAATCCAAGG ACCTGACCAC GGCTCCCTGT TGCACCACGC TGCCAAGAGT 
GGGCATGGCG AGATTGTGAA GTATATCCTG GAACATGGTC CATCTGAATT ACTGGACATG 
ACAGACAGTG AAACGGGTGA GACAGCATTA CATAAAGCGG CCTGCCAACG CCATCGTGCC 
ATCTGCCAGC TCCTGGTGGA TGCGGGAGCC TCGCTGAGAA AGACGGACTC CAAGGGTAAG 
ACTCCTCGTG ACAGGGCTCA GCAAGCTGGT GATCCAGATC TGGCCTCATA TCTGGAGAGT 
CGACAGAATT ACCAAATGGT TCCCCATGAG GATTTGGAAA CAGCAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_025156061.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025011829.1 Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OGa142748
Clone ID Related Accession (Same CDS sequence)	XM_025156061.1
Accession Version	XM_025156061.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGG GATGCATGGA TTTCCTTTGC AAAAGCAGCA TGAAGCAGAA CAGGTGTAAA 
TTGTCTGTGC CGGGCTCCTT GGATAGCTGG AAAGCAATCT CCAGGTCAGG CCTCCAGCAC 
TTGGCTCCTG TGCATTCCCT CAACATTGCA GTCTCCAATG GACCTGTGAA GGAACCAAGG 
GCAGCCTTAG AGTGGACTGA AAATGCTGTT AACGGAGAGC ATCTATGGTT GGAGACAAAT 
GTTTCTGGGG ACCTCTGTTA CCTGGGGGAG GAAAGCTGCC AAGTGAAATT CTCTAAATCT 
GCTCTGAGGA GGAAGTGTGC TGCCTGCAAG ATTGTGGTCC ACAACGCATG CATGGAGCAG 
TTAGAGAAGA TTAATTTTCG CTGCAAACCA ACTTTCCGAG AAGGGAGCTC CAGGTCTCCA 
AGAGAAAACT TTGTCCGACA TCACTGGGTA CACAGACGGA GGCAGGAGGG GAAATGTAAG 
CAGTGTGGAA AGGGCTTTCA GCAGAAATTC TCCTTCCATA GTAAAGAGAT TGTGGCCATC 
AGTTGTTCCT GGTGCAAGCT GGCGTTTCAT AACAAAGTCA CCTGCTTCAT GCTACATCAC 
ATTGAGGAGC CATGTTCTCT GGGGGCTCAC GCGGCTGTCA TTGTGCCTCC CACCTGGATC 
ATTAAGGTGA AGAAACCTCA GGGGGTCTCC ATACCAGGAG ACGAGCCTGA TTGGGCAGGA 
GACTGGAGCC CCGAAGGGGA AACGGGCGTC TCCTACCCCT ATTGGGAGGG TTGGCAGAAC 
TCATTAAAGA CTTCAACACG GCGAAAGAAG AGGACATCTT TTAAAAGAAA AGCCAGCAAA 
AGAGGCAATG ATGATAACAA AGGTCGGCCA TTCGTGATAA AGCCTATTTC CTCTCCACTC 
ATGAAACCAC TGTTGGTTTT TGTAAATCCA AAAAGTGGAG GGAACCAGGG CACCAAAGTT 
CTCCAAATGT TTATGTGGTA TTTGAATCCA CGCCAGGTCT TTGATCTCTC TCAGGAAGGG 
CCAAAAGATG CGCTGGAGCT GTACAGAAAA GTGCCAAACC TGCGGATCCT GGCCTGTGGC 
GGGGATGGAA CGGTTGGCTG GATCCTCTCT ATCCTAGATG AGCTGCAACT CACTCCTCAG 
CCTCCTGTGG CTGTCCTTCC ACTTGGCACT GGGAATGACC TTGCCCGCAC CCTCAACTGG 
GGTGGGGGTT ACACAGATGA GCCTGTGTCC AAGATCTTGT GTCATGTAGA AGATGGGACC 
ATTGTCCAGC TGGACCGATG GAATCTACAA GTTGAGCGCA ACCCAGATTT GCCACAGGAT 
GAGCTGGAAG ATGGGTCACG TAAGCTTCCA CTCAATGTCT TCAATAATTA CTTCAGCCTT 
GGGTTTGATG CACATGTTAC ACTGGAGTTC CACGAATCCA GAGAAGCAAA TCCAGAGAAA 
TTCAACAGCC GATTTAGAAA TAAAATGTTT TATGCAGGGG CAGCCTTTTC AGACTTCTTG 
CAAAGAAGCT CAAGAGATTT ATCCAAGCAC GTTAAAGTTG TGTGTGATGG TACAGACCTG 
ACTCCAAAGA TCCAGGAGCT GAAGTTCCAG TGTATAGTGT TTTTAAACAT TCCCAGGTAT 
TGTGCTGGCA CAATGCCCTG GGGAAACCCT GGAGATCACA GAGACTTTGA ACCTCAGCGC 
CATGATGATG GCTACATTGA AGTCATTGGG TTCACCATGG CCTCACTGGC TGCTCTCCAA 
GTGGGTGGTC ATGGAGAGAG GTTGCATCAG TGCCGTGAGG TGACGCTTTT AACGTACAAG 
TCTATCCCCA TGCAAGTGGA TGGTGAACCT TGTCGGTTGG CTCCCTCACT CATTCGCATC 
TCCTTAAGGA ACCAAGCCAA CATGGTGCAG AAGAGCAAGA GGAGAACATC CATGCCTTTG 
TTGAATGATA TCCATAAAGT GCAGTCTGCT GACCTGAGGA GAGTCTCAGC TCCCCCCGAT 
TCCTTCACTG TTCCTCATTC CATCCCTGAT CGCCTGCGGA TCAGAGTGAA CCGGATTAAT 
TTGCAAGAAT ATGAGGGGCT ACATTATGAC AAGGAAAAAC TCCGTGAAGC TTCCATTCCC 
TTAGGAATTA TAGTTGTACG AGGAGATTGT GACTTGGAGA CCTGTCGTAT GTATATTGAC 
CGTCTACAAG AGGATCTACA GTCAGCAACT TCTACTACAC AGAGAGTTCA TTACCAGGAC 
CAAGAAAGCT CTTTCCCAAG AGTACTTTCT GTTCAGAGGC TCTCTCCTAG ATGGTGCTTC 
CTAGATGCAA CTTCTGCTGA TCGTTTTTAC CGCATTGACA GATCTCAGGA ACATTTGCAC 
TTTGTGACGG AAATTTCGCA GGACGAGATT TTTATTCTGG ACCCAGAGCT GGTAATGTCA 
CAGCAGGGGG GGACGCCACC TGGAATGCCT GATCTGGTAG TGGAGCAGGC CTCTGGAATA 
TCAGATCGGT GGAACCCTGC TGTTCGAAAG AGGATGCTGA GCGACAGTGG CCTTGGCAAG 
ATTTCCCCAC ACTACGAGGT ACCTGACAAA CAAAAGGACG CCAGCCAGAC CTATATACTG 
CAGTCACCAG TTTCTTCAGA AGATCAAGCA CTTTTACAGG CAGTCATAGC TGGTGATCTG 
CTGAAGTTCA TAGAATGCTG TAAAAAAGGA GCCAATTTGC TAATCCAAGG ACCTGACCAC 
GGCTCCCTGT TGCACCACGC TGCCAAGAGT GGGCATGGCG AGATTGTGAA GTATATCCTG 
GAACATGGTC CATCTGAATT ACTGGACATG ACAGACAGTG AAACGGGTGA GACAGCATTA 
CATAAAGCGG CCTGCCAACG CCATCGTGCC ATCTGCCAGC TCCTGGTGGA TGCGGGAGCC 
TCGCTGAGAA AGACGGACTC CAAGGGTAAG ACTCCTCGTG ACAGGGCTCA GCAAGCTGGT 
GATCCAGATC TGGCCTCATA TCTGGAGAGT CGACAGAATT ACCAAATGGT TCCCCATGAG 
GATTTGGAAA CAGCAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025011829.1
CDS	331..3351
Translation	MSVGCMDFLCKSSMKQNRCKLSVPGSLDSWKAISRSGLQHLAPVHSLNIAVSNGPVKEPRAALEWTENAVNGEHLWLETNVSGDLCYLGEESCQVKFSKSALRRKCAACKIVVHNACMEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQGVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDIHKVQSADLRRVSAPPDSFTVPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_025156061.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase iota (DGKI), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025156061.1

ORF Insert Sequence:

ATGAGTGTGG GATGCATGGA TTTCCTTTGC AAAAGCAGCA TGAAGCAGAA CAGGTGTAAA 
TTGTCTGTGC CGGGCTCCTT GGATAGCTGG AAAGCAATCT CCAGGTCAGG CCTCCAGCAC 
TTGGCTCCTG TGCATTCCCT CAACATTGCA GTCTCCAATG GACCTGTGAA GGAACCAAGG 
GCAGCCTTAG AGTGGACTGA AAATGCTGTT AACGGAGAGC ATCTATGGTT GGAGACAAAT 
GTTTCTGGGG ACCTCTGTTA CCTGGGGGAG GAAAGCTGCC AAGTGAAATT CTCTAAATCT 
GCTCTGAGGA GGAAGTGTGC TGCCTGCAAG ATTGTGGTCC ACAACGCATG CATGGAGCAG 
TTAGAGAAGA TTAATTTTCG CTGCAAACCA ACTTTCCGAG AAGGGAGCTC CAGGTCTCCA 
AGAGAAAACT TTGTCCGACA TCACTGGGTA CACAGACGGA GGCAGGAGGG GAAATGTAAG 
CAGTGTGGAA AGGGCTTTCA GCAGAAATTC TCCTTCCATA GTAAAGAGAT TGTGGCCATC 
AGTTGTTCCT GGTGCAAGCT GGCGTTTCAT AACAAAGTCA CCTGCTTCAT GCTACATCAC 
ATTGAGGAGC CATGTTCTCT GGGGGCTCAC GCGGCTGTCA TTGTGCCTCC CACCTGGATC 
ATTAAGGTGA AGAAACCTCA GGGGGTCTCC ATACCAGGAG ACGAGCCTGA TTGGGCAGGA 
GACTGGAGCC CCGAAGGGGA AACGGGCGTC TCCTACCCCT ATTGGGAGGG TTGGCAGAAC 
TCATTAAAGA CTTCAACACG GCGAAAGAAG AGGACATCTT TTAAAAGAAA AGCCAGCAAA 
AGAGGCAATG ATGATAACAA AGGTCGGCCA TTCGTGATAA AGCCTATTTC CTCTCCACTC 
ATGAAACCAC TGTTGGTTTT TGTAAATCCA AAAAGTGGAG GGAACCAGGG CACCAAAGTT 
CTCCAAATGT TTATGTGGTA TTTGAATCCA CGCCAGGTCT TTGATCTCTC TCAGGAAGGG 
CCAAAAGATG CGCTGGAGCT GTACAGAAAA GTGCCAAACC TGCGGATCCT GGCCTGTGGC 
GGGGATGGAA CGGTTGGCTG GATCCTCTCT ATCCTAGATG AGCTGCAACT CACTCCTCAG 
CCTCCTGTGG CTGTCCTTCC ACTTGGCACT GGGAATGACC TTGCCCGCAC CCTCAACTGG 
GGTGGGGGTT ACACAGATGA GCCTGTGTCC AAGATCTTGT GTCATGTAGA AGATGGGACC 
ATTGTCCAGC TGGACCGATG GAATCTACAA GTTGAGCGCA ACCCAGATTT GCCACAGGAT 
GAGCTGGAAG ATGGGTCACG TAAGCTTCCA CTCAATGTCT TCAATAATTA CTTCAGCCTT 
GGGTTTGATG CACATGTTAC ACTGGAGTTC CACGAATCCA GAGAAGCAAA TCCAGAGAAA 
TTCAACAGCC GATTTAGAAA TAAAATGTTT TATGCAGGGG CAGCCTTTTC AGACTTCTTG 
CAAAGAAGCT CAAGAGATTT ATCCAAGCAC GTTAAAGTTG TGTGTGATGG TACAGACCTG 
ACTCCAAAGA TCCAGGAGCT GAAGTTCCAG TGTATAGTGT TTTTAAACAT TCCCAGGTAT 
TGTGCTGGCA CAATGCCCTG GGGAAACCCT GGAGATCACA GAGACTTTGA ACCTCAGCGC 
CATGATGATG GCTACATTGA AGTCATTGGG TTCACCATGG CCTCACTGGC TGCTCTCCAA 
GTGGGTGGTC ATGGAGAGAG GTTGCATCAG TGCCGTGAGG TGACGCTTTT AACGTACAAG 
TCTATCCCCA TGCAAGTGGA TGGTGAACCT TGTCGGTTGG CTCCCTCACT CATTCGCATC 
TCCTTAAGGA ACCAAGCCAA CATGGTGCAG AAGAGCAAGA GGAGAACATC CATGCCTTTG 
TTGAATGATA TCCATAAAGT GCAGTCTGCT GACCTGAGGA GAGTCTCAGC TCCCCCCGAT 
TCCTTCACTG TTCCTCATTC CATCCCTGAT CGCCTGCGGA TCAGAGTGAA CCGGATTAAT 
TTGCAAGAAT ATGAGGGGCT ACATTATGAC AAGGAAAAAC TCCGTGAAGC TTCCATTCCC 
TTAGGAATTA TAGTTGTACG AGGAGATTGT GACTTGGAGA CCTGTCGTAT GTATATTGAC 
CGTCTACAAG AGGATCTACA GTCAGCAACT TCTACTACAC AGAGAGTTCA TTACCAGGAC 
CAAGAAAGCT CTTTCCCAAG AGTACTTTCT GTTCAGAGGC TCTCTCCTAG ATGGTGCTTC 
CTAGATGCAA CTTCTGCTGA TCGTTTTTAC CGCATTGACA GATCTCAGGA ACATTTGCAC 
TTTGTGACGG AAATTTCGCA GGACGAGATT TTTATTCTGG ACCCAGAGCT GGTAATGTCA 
CAGCAGGGGG GGACGCCACC TGGAATGCCT GATCTGGTAG TGGAGCAGGC CTCTGGAATA 
TCAGATCGGT GGAACCCTGC TGTTCGAAAG AGGATGCTGA GCGACAGTGG CCTTGGCAAG 
ATTTCCCCAC ACTACGAGGT ACCTGACAAA CAAAAGGACG CCAGCCAGAC CTATATACTG 
CAGTCACCAG TTTCTTCAGA AGATCAAGCA CTTTTACAGG CAGTCATAGC TGGTGATCTG 
CTGAAGTTCA TAGAATGCTG TAAAAAAGGA GCCAATTTGC TAATCCAAGG ACCTGACCAC 
GGCTCCCTGT TGCACCACGC TGCCAAGAGT GGGCATGGCG AGATTGTGAA GTATATCCTG 
GAACATGGTC CATCTGAATT ACTGGACATG ACAGACAGTG AAACGGGTGA GACAGCATTA 
CATAAAGCGG CCTGCCAACG CCATCGTGCC ATCTGCCAGC TCCTGGTGGA TGCGGGAGCC 
TCGCTGAGAA AGACGGACTC CAAGGGTAAG ACTCCTCGTG ACAGGGCTCA GCAAGCTGGT 
GATCCAGATC TGGCCTCATA TCTGGAGAGT CGACAGAATT ACCAAATGGT TCCCCATGAG 
GATTTGGAAA CAGCAGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_015286157.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015141643.1 Gallus gallus diacylglycerol kinase, iota (DGKI), mRNA.

Price & Availability↑

CloneID	OGa29385
Clone ID Related Accession (Same CDS sequence)	XM_015286157.1
Accession Version	XM_015286157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	diacylglycerol kinase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 7% of CDS bases ##RefSeq-Attributes-END##

ATGCCCCGCT GCAGTGCGGC CGCCGATGGA TGCTGCGCGG CTGCGCTGCC ATTTGCAGCC 
GCCGCGGCTC GCAGCGCGCC CCGGGGCCCC GGCAGCGGTC GCCCCCCGAC CCCCGACGGC 
CCCCAGCGCC TGCAGCCGCC GGCCACCCCC CGCCTGCCCC CTCCTTCTCC TTCGCGGCCC 
CTCTGCCCCG CCGCAATGAA CCCCCCCGCC GCGGCCGGCG GGGAGGAGAC GGGGGCGGCG 
GGCGGCGGCT GCCGGGGGGC CGAGGGCGGC GGGGAGGCGG CGGGTGCCGC CGGGGTCCCG 
GAGGAGCCGG GGCTGGCGGC GGCGGAGGAG TCGCTGGAGG AGAAGCTGCG GAACCTCACC 
TTCAGGAAGC AGGTGTCCTA CAGGAAAGCA ATCTCCAGGT CAGGCCTCCA GCACTTGGCT 
CCTGTGCATT CCCTCAACAT TGCAGTCTCC AATGGACCTG TGAAGGAACC AAGGGCAGCC 
TTAGAGTGGA CTGAAAATGC TGTTAACGGA GAGCATCTAT GGTTGGAGAC AAATGTTTCT 
GGGGACCTCT GTTACCTGGG GGAGGAAAGC TGCCAAGTGA AATTCTCTAA ATCTGCTCTG 
AGGAGGAAGT GTGCTGCCTG CAAGATTGTG GTCCACAACG CATGCATGGA GCAGTTAGAG 
AAGATTAATT TTCGCTGCAA ACCAACTTTC CGAGAAGGGA GCTCCAGGTC TCCAAGAGAA 
AACTTTGTCC GACATCACTG GGTACACAGA CGGAGGCAGG AGGGGAAATG TAAGCAGTGT 
GGAAAGGGCT TTCAGCAGAA ATTCTCCTTC CATAGTAAAG AGATTGTGGC CATCAGTTGT 
TCCTGGTGCA AGCTGGCGTT TCATAACAAA GTCACCTGCT TCATGCTACA TCACATTGAG 
GAGCCATGTT CTCTGGGGGC TCACGCGGCT GTCATTGTGC CTCCCACCTG GATCATTAAG 
GTGAAGAAAC CTCAGGGGGT CTCCATACCA GGAGACGAGC CTGATTGGGC AGGAGACTGG 
AGCCCCGAAG GGGAAACGGG CGTCTCCTAC CCCTATTGGG AGGGTTGGCA GAACTCATTA 
AAGACTTCAA CACGGCGAAA GAAGAGGACA TCTTTTAAAA GAAAAGCCAG CAAAAGAGGC 
AATGATGATA ACAAAGGTCG GCCATTCGTG ATAAAGCCTA TTTCCTCTCC ACTCATGAAA 
CCACTGTTGG TTTTTGTAAA TCCAAAAAGT GGAGGGAACC AGGGCACCAA AGTTCTCCAA 
ATGTTTATGT GGTATTTGAA TCCACGCCAG GTCTTTGATC TCTCTCAGGA AGGGCCAAAA 
GATGCGCTGG AGCTGTACAG AAAAGTGCCA AACCTGCGGA TCCTGGCCTG TGGCGGGGAT 
GGAACGGTTG GCTGGATCCT CTCTATCCTA GATGAGCTGC AACTCACTCC TCAGCCTCCT 
GTGGCTGTCC TTCCACTTGG CACTGGGAAT GACCTTGCCC GCACCCTCAA CTGGGGTGGG 
GGTTACACAG ATGAGCCTGT GTCCAAGATC TTGTGTCATG TAGAAGATGG GACCATTGTC 
CAGCTGGACC GATGGAATCT ACAAGTTGAG CGCAACCCAG ATTTGCCACA GGATGAGCTG 
GAAGATGGGT CACGTAAGCT TCCACTCAAT GTCTTCAATA ATTACTTCAG CCTTGGGTTT 
GATGCACATG TTACACTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGCCGATTTA GAAATAAAAT GTTTTATGCA GGGGCAGCCT TTTCAGACTT CTTGCAAAGA 
AGCTCAAGAG ATTTATCCAA GCACGTTAAA GTTGTGTGTG ATGGTACAGA CCTGACTCCA 
AAGATCCAGG AGCTGAAGTT CCAGTGTATA GTGTTTTTAA ACATTCCCAG GTATTGTGCT 
GGCACAATGC CCTGGGGAAA CCCTGGAGAT CACAGAGACT TTGAACCTCA GCGCCATGAT 
GATGGCTACA TTGAAGTCAT TGGGTTCACC ATGGCCTCAC TGGCTGCTCT CCAAGTGGGT 
GGTCATGGAG AGAGGTTGCA TCAGTGCCGT GAGGTGACGC TTTTAACGTA CAAGTCTATC 
CCCATGCAAG TGGATGGTGA ACCTTGTCGG TTGGCTCCCT CACTCATTCG CATCTCCTTA 
AGGAACCAAG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CATCCATGCC TTTGTTGAAT 
GATATCCATA AAGTGCAGTC TGCTGACCTG AGGAGAGTCT CAGCTCCCCC CGATTCCTTC 
ACTGTTCCTC ATTCCATCCC TGATCGCCTG CGGATCAGAG TGAACCGGAT TAATTTGCAA 
GAATATGAGG GGCTACATTA TGACAAGGAA AAACTCCGTG AAGCTTCCAT TCCCTTAGGA 
ATTATAGTTG TACGAGGAGA TTGTGACTTG GAGACCTGTC GTATGTATAT TGACCGTCTA 
CAAGAGGATC TACAGTCAGC AACTTCTACT ACACAGAGAG TTCATTACCA GGACCAAGAA 
AGCTCTTTCC CAAGAGTACT TTCTGTTCAG AGGCTCTCTC CTAGATGGTG CTTCCTAGAT 
GCAACTTCTG CTGATCGTTT TTACCGCATT GACAGATCTC AGGAACATTT GCACTTTGTG 
ACGGAAATTT CGCAGGACGA GATTTTTATT CTGGACCCAG AGCTGGTAAT GTCACAGCAG 
GGGGGGACGC CACCTGGAAT GCCTGATCTG GTAGTGGAGC AGGCCTCTGG AATATCAGAT 
CGGTGGAACC CTGCTGTTCG AAAGAGGATG CTGAGCGACA GTGGCCTTGG CAAGATTTCC 
CCACACTACG AGGTACCTGA CAAACAAAAG GACGCCAGCC AGACCTATAT ACTGCAGTCA 
CCAGTTTCTT CAGAAGATCA AGCACTTTTA CAGGCAGTCA TAGCTGGTGA TCTGCTGAAG 
TTCATAGAAT GCTGTAAAAA AGGAGCCAAT TTGCTAATCC AAGGACCTGA CCACGGCTCC 
CTGTTGCACC ACGCTGCCAA GAGTGGGCAT GGCGAGATTG TGAAGTATAT CCTGGAACAT 
GGTCCATCTG AATTACTGGA CATGACAGAC AGTGAAACGG GTGAGACAGC ATTACATAAA 
GCGGCCTGCC AACGCCATCG TGCCATCTGC CAGCTCCTGG TGGATGCGGG AGCCTCGCTG 
AGAAAGACGG ACTCCAAGGG TAAGACTCCT CGTGACAGGG CTCAGCAAGC TGGTGATCCA 
GATCTGGCCT CATATCTGGA GAGTCGACAG AATTACCAAA TGGTTCCCCA TGAGGATTTG 
GAAACAGCAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015141643.1
CDS	1..3315
Translation	MPRCSAAADGCCAAALPFAAAAARSAPRGPGSGRPPTPDGPQRLQPPATPRLPPPSPSRPLCPAAMNPPAAAGGEETGAAGGGCRGAEGGGEAAGAAGVPEEPGLAAAEESLEEKLRNLTFRKQVSYRKAISRSGLQHLAPVHSLNIAVSNGPVKEPRAALEWTENAVNGEHLWLETNVSGDLCYLGEESCQVKFSKSALRRKCAACKIVVHNACMEQLEKINFRCKPTFREGSSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKLAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQGVSIPGDEPDWAGDWSPEGETGVSYPYWEGWQNSLKTSTRRKKRTSFKRKASKRGNDDNKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLTPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKILCHVEDGTIVQLDRWNLQVERNPDLPQDELEDGSRKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHRDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVTLLTYKSIPMQVDGEPCRLAPSLIRISLRNQANMVQKSKRRTSMPLLNDIHKVQSADLRRVSAPPDSFTVPHSIPDRLRIRVNRINLQEYEGLHYDKEKLREASIPLGIIVVRGDCDLETCRMYIDRLQEDLQSATSTTQRVHYQDQESSFPRVLSVQRLSPRWCFLDATSADRFYRIDRSQEHLHFVTEISQDEIFILDPELVMSQQGGTPPGMPDLVVEQASGISDRWNPAVRKRMLSDSGLGKISPHYEVPDKQKDASQTYILQSPVSSEDQALLQAVIAGDLLKFIECCKKGANLLIQGPDHGSLLHHAAKSGHGEIVKYILEHGPSELLDMTDSETGETALHKAACQRHRAICQLLVDAGASLRKTDSKGKTPRDRAQQAGDPDLASYLESRQNYQMVPHEDLETAV

Target ORF information:

RefSeq Version	XM_015286157.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus diacylglycerol kinase, iota (DGKI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015286157.1

ORF Insert Sequence:

ATGCCCCGCT GCAGTGCGGC CGCCGATGGA TGCTGCGCGG CTGCGCTGCC ATTTGCAGCC 
GCCGCGGCTC GCAGCGCGCC CCGGGGCCCC GGCAGCGGTC GCCCCCCGAC CCCCGACGGC 
CCCCAGCGCC TGCAGCCGCC GGCCACCCCC CGCCTGCCCC CTCCTTCTCC TTCGCGGCCC 
CTCTGCCCCG CCGCAATGAA CCCCCCCGCC GCGGCCGGCG GGGAGGAGAC GGGGGCGGCG 
GGCGGCGGCT GCCGGGGGGC CGAGGGCGGC GGGGAGGCGG CGGGTGCCGC CGGGGTCCCG 
GAGGAGCCGG GGCTGGCGGC GGCGGAGGAG TCGCTGGAGG AGAAGCTGCG GAACCTCACC 
TTCAGGAAGC AGGTGTCCTA CAGGAAAGCA ATCTCCAGGT CAGGCCTCCA GCACTTGGCT 
CCTGTGCATT CCCTCAACAT TGCAGTCTCC AATGGACCTG TGAAGGAACC AAGGGCAGCC 
TTAGAGTGGA CTGAAAATGC TGTTAACGGA GAGCATCTAT GGTTGGAGAC AAATGTTTCT 
GGGGACCTCT GTTACCTGGG GGAGGAAAGC TGCCAAGTGA AATTCTCTAA ATCTGCTCTG 
AGGAGGAAGT GTGCTGCCTG CAAGATTGTG GTCCACAACG CATGCATGGA GCAGTTAGAG 
AAGATTAATT TTCGCTGCAA ACCAACTTTC CGAGAAGGGA GCTCCAGGTC TCCAAGAGAA 
AACTTTGTCC GACATCACTG GGTACACAGA CGGAGGCAGG AGGGGAAATG TAAGCAGTGT 
GGAAAGGGCT TTCAGCAGAA ATTCTCCTTC CATAGTAAAG AGATTGTGGC CATCAGTTGT 
TCCTGGTGCA AGCTGGCGTT TCATAACAAA GTCACCTGCT TCATGCTACA TCACATTGAG 
GAGCCATGTT CTCTGGGGGC TCACGCGGCT GTCATTGTGC CTCCCACCTG GATCATTAAG 
GTGAAGAAAC CTCAGGGGGT CTCCATACCA GGAGACGAGC CTGATTGGGC AGGAGACTGG 
AGCCCCGAAG GGGAAACGGG CGTCTCCTAC CCCTATTGGG AGGGTTGGCA GAACTCATTA 
AAGACTTCAA CACGGCGAAA GAAGAGGACA TCTTTTAAAA GAAAAGCCAG CAAAAGAGGC 
AATGATGATA ACAAAGGTCG GCCATTCGTG ATAAAGCCTA TTTCCTCTCC ACTCATGAAA 
CCACTGTTGG TTTTTGTAAA TCCAAAAAGT GGAGGGAACC AGGGCACCAA AGTTCTCCAA 
ATGTTTATGT GGTATTTGAA TCCACGCCAG GTCTTTGATC TCTCTCAGGA AGGGCCAAAA 
GATGCGCTGG AGCTGTACAG AAAAGTGCCA AACCTGCGGA TCCTGGCCTG TGGCGGGGAT 
GGAACGGTTG GCTGGATCCT CTCTATCCTA GATGAGCTGC AACTCACTCC TCAGCCTCCT 
GTGGCTGTCC TTCCACTTGG CACTGGGAAT GACCTTGCCC GCACCCTCAA CTGGGGTGGG 
GGTTACACAG ATGAGCCTGT GTCCAAGATC TTGTGTCATG TAGAAGATGG GACCATTGTC 
CAGCTGGACC GATGGAATCT ACAAGTTGAG CGCAACCCAG ATTTGCCACA GGATGAGCTG 
GAAGATGGGT CACGTAAGCT TCCACTCAAT GTCTTCAATA ATTACTTCAG CCTTGGGTTT 
GATGCACATG TTACACTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGCCGATTTA GAAATAAAAT GTTTTATGCA GGGGCAGCCT TTTCAGACTT CTTGCAAAGA 
AGCTCAAGAG ATTTATCCAA GCACGTTAAA GTTGTGTGTG ATGGTACAGA CCTGACTCCA 
AAGATCCAGG AGCTGAAGTT CCAGTGTATA GTGTTTTTAA ACATTCCCAG GTATTGTGCT 
GGCACAATGC CCTGGGGAAA CCCTGGAGAT CACAGAGACT TTGAACCTCA GCGCCATGAT 
GATGGCTACA TTGAAGTCAT TGGGTTCACC ATGGCCTCAC TGGCTGCTCT CCAAGTGGGT 
GGTCATGGAG AGAGGTTGCA TCAGTGCCGT GAGGTGACGC TTTTAACGTA CAAGTCTATC 
CCCATGCAAG TGGATGGTGA ACCTTGTCGG TTGGCTCCCT CACTCATTCG CATCTCCTTA 
AGGAACCAAG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CATCCATGCC TTTGTTGAAT 
GATATCCATA AAGTGCAGTC TGCTGACCTG AGGAGAGTCT CAGCTCCCCC CGATTCCTTC 
ACTGTTCCTC ATTCCATCCC TGATCGCCTG CGGATCAGAG TGAACCGGAT TAATTTGCAA 
GAATATGAGG GGCTACATTA TGACAAGGAA AAACTCCGTG AAGCTTCCAT TCCCTTAGGA 
ATTATAGTTG TACGAGGAGA TTGTGACTTG GAGACCTGTC GTATGTATAT TGACCGTCTA 
CAAGAGGATC TACAGTCAGC AACTTCTACT ACACAGAGAG TTCATTACCA GGACCAAGAA 
AGCTCTTTCC CAAGAGTACT TTCTGTTCAG AGGCTCTCTC CTAGATGGTG CTTCCTAGAT 
GCAACTTCTG CTGATCGTTT TTACCGCATT GACAGATCTC AGGAACATTT GCACTTTGTG 
ACGGAAATTT CGCAGGACGA GATTTTTATT CTGGACCCAG AGCTGGTAAT GTCACAGCAG 
GGGGGGACGC CACCTGGAAT GCCTGATCTG GTAGTGGAGC AGGCCTCTGG AATATCAGAT 
CGGTGGAACC CTGCTGTTCG AAAGAGGATG CTGAGCGACA GTGGCCTTGG CAAGATTTCC 
CCACACTACG AGGTACCTGA CAAACAAAAG GACGCCAGCC AGACCTATAT ACTGCAGTCA 
CCAGTTTCTT CAGAAGATCA AGCACTTTTA CAGGCAGTCA TAGCTGGTGA TCTGCTGAAG 
TTCATAGAAT GCTGTAAAAA AGGAGCCAAT TTGCTAATCC AAGGACCTGA CCACGGCTCC 
CTGTTGCACC ACGCTGCCAA GAGTGGGCAT GGCGAGATTG TGAAGTATAT CCTGGAACAT 
GGTCCATCTG AATTACTGGA CATGACAGAC AGTGAAACGG GTGAGACAGC ATTACATAAA 
GCGGCCTGCC AACGCCATCG TGCCATCTGC CAGCTCCTGG TGGATGCGGG AGCCTCGCTG 
AGAAAGACGG ACTCCAAGGG TAAGACTCCT CGTGACAGGG CTCAGCAAGC TGGTGATCCA 
GATCTGGCCT CATATCTGGA GAGTCGACAG AATTACCAAA TGGTTCCCCA TGAGGATTTG 
GAAACAGCAG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DGKI cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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