DGKI cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	100331649
Full Name	diacylglycerol kinase, iota
General protein information	Preferred Names diacylglycerol kinase, iota Names diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002666976.5	XP_002667022.3	diacylglycerol kinase iota isoform X4
XM_009300037.3	XP_009298312.1	diacylglycerol kinase iota
XM_009300037.2	XP_009298312.1	diacylglycerol kinase iota isoform X1
XM_009300041.2	XP_009298316.1	diacylglycerol kinase iota isoform X5
XM_009300038.2	XP_009298313.1	diacylglycerol kinase iota isoform X2
XM_009300039.2	XP_009298314.1	diacylglycerol kinase iota isoform X3

Pathways from BioSystems

Homologies

	DGKI	XP_001107030.1
Canis lupus familiaris (dog)	DGKI	XP_005629798.1
Bos taurus (cattle)	DGKI	XP_002687107.1
Rattus norvegicus (Norway rat)	Dgki	NP_942077.2
Danio rerio (zebrafish)	dgki	XP_002667022.3
Xenopus tropicalis (tropical clawed frog)	dgki	XP_002942047.2
Gallus gallus (chicken)	DGKI	XP_416357.4
Homo sapiens (human)	DGKI	NP_004708.1
Pan troglodytes (chimpanzee)	DGKI	XP_001147695.1
Mus musculus (house mouse)	Dgki	NP_001074675.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa09128	XM_002666976.5 Latest version!	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49499	XM_009300037.3 Latest version!	Danio rerio diacylglycerol kinase, iota (dgki), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49500	XM_009300038.2 Latest version!	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49501	XM_009300039.2 Latest version!	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa49503	XM_009300041.2 Latest version!	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa49499	XM_009300037.2	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_002666976.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002667022.3 Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa09128
Clone ID Related Accession (Same CDS sequence)	XM_002666976.5
Accession Version	XM_002666976.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase iota isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666976.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACC AGAATGTGTT GCGGCACCAC TGGGTTCATC GTCGCCGACA GGAGGGAAAA 
TGTCGACAGT GCGGCAAAAG TTTTCCACAG AAGTTCTTCC ACAGTAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA ACAGGCGTTC CACAACAAGG TGACGTGTTT CATGCTGCAT 
CAGATTGAGG AGCCCTGTTC ACTGGGAGCT CACGCCGGGG TCATTGTCCC ACCATCCTGG 
ATTATTAAAG TCCGGAAACC GCAGAGCTCA TTTAAAAACT CCACTCGGAG GAAAAAACGG 
ACGTCGTTCA AAAGAAGAGC CAGCAAAAAG GGCACGGATG AATCTAAATG GCGTCCATTC 
ATGCTGAAGC CGCTGCCGTC ACCGCTGATG AAACCAGTGC TGGTGTTCGT CAACCCGAAA 
AGTGGAGGAA ACCAGGGCAC GAAGCTCCTG CAGATGTTCA TGTGGATCCT GAACCCTCGG 
CAGGTGTTTG ATTTGTCTCA GGGAGGCCCC AGAGATGCGT TGGAGTTGTA CAGGAAAGTG 
CCAAATCTTC GCATCCTGGC CTGTGGAGGA GATGGAACCG TGGGCTGGAT TCTGTCGGCT 
TTAGATGAGC TTCAGATGAA CCCTCAGCCT CCTGTGGCGG TACTTCCTCT TGGAACAGGA 
AATGACCTTG CTAGGACACT CAACTGGGGA GGTGGCTACA CAGACGAGCC GGTGTCGAAG 
GTGTTGTGTC ATGTGGAGGA CGGCTCTGTG GTCCAGCTGG ACCGCTGGAA TCTGCAGGTG 
GAGCGCTCAG TGGTCCAGCA CGAGGAGGGA ACACAGAAGC TTCCTTTAAA TGTGTTCAAT 
AACTACTTCA GCCTCGGTTT CGATGCCCAC GTCACCCTGG AGTTTCACGA GTCCAGAGAA 
GCAAACCCAG AGAAATTCAA CAGTCGCTTC CGTAACAAGA TGTTCTATGC AGGGGCTGCG 
TTCTCAGACT TCCTCCAGAG GAGCTCCAGG GATCTGTCTA AGCATGTCCG AGTTGTGTGT 
GACGGCACCG ATCTCACAGC CAAGATCCAG GAGCTCAAGT TTCAGTGCAT CGTCTTCTTG 
AATATTCCCA GATACTGTGC TGGAACAATG CCATGGGGAA ACACAGGAGA CCACCGAGAC 
TTTGAGCCAC AGCGACATGA TGATGGCTGC ATCGAGGTCA TCGGCTTCAC CATGGCCTCT 
CTGGCGGCGC TGCAGGTTGG TGGTCATGGG GATCGTCTCC ATCAGTGTCG AGAGGTGCTC 
ATCACCACCT TCAAGACTCT GCCAGTGCAG GTAGATGGAG AGCCGTGCCG TCTGGCGCCC 
TCCACCCTGC GCATCTCTCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGAGACGC 
ACATCTGTAC CGCTGCTCAA TGACCCTCAC GCCGTTCCAG AGCGCCTGCG TCTGCGTGTC 
AACCGCATCG GTCTGCAGGA CTACGACAGC ATGCAGTTCG ATAAGGAACG TCTGCGCGAC 
ATCTCGACTG CTGTTGGCAT CGTGGTGGTG AGAGGAGACT GTGACCTCGA AACCTGTCGA 
CTCTACATTG ATAGACTGCA GGAGGATTTG CATCAGGCGC CCTCTGCTGG CCACCGAGTG 
CACTATCAGG ATGAGTGTGC AGGTTTGCCA CGGCCGAGTT CAGCACACAG ACTTTCAGCC 
AACTGGAGCT TCCTGGACTC TACATCAGCT GATCGCTTTT ATCGCATCGA CAAGGCTCAG 
GAGCATCTTC ACTTTGTAAC TGAAATATGC CAGGATGAGG TTTATATCCT GGACCATGAA 
GCCCCAGCGG TGAGCCAGGT CAGGGCTCCA GGAATGCCTG ATGTGGTGGT GGAGCCCAGT 
TCTGGTGTCC CTCTGACATC AGATGAGCAA GCTCTGCTGT CGGCGGCTGC GAAAGGGGAC 
TTGACTGCGG TGTCATCGTC CTGCAGGAGT GGGCTTAGTC TGCTGGTGCG GGACGCTATG 
GGCTGCACCG CTCTGCATTT GGCTGCACAT CACGGACACG CAGACGTGGT GAGCTTCATT 
CTGGAGCATG GGTCAAAGCT GATGTTGGAT TTAACAGAGC GAGACACAGG TGACACAGCA 
TTGCACAAAG CGGCGGCACA ACAACAGCGT GATGTTTGCA AACGTCTGCT TGAAGCCGGA 
GCTTCCCCCC ACAAAACCAA CTTCCTGGGG AAAACACCTA AGGATTGTGC CCTGGATGCC 
GGGAATTTAG AGTTGGCGTC CATGCTGGAG ATCCAGGCGG TGGATGAACC AGCATCACAC 
GAGGATCTGG AGACGGCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667022.3
CDS	553..3396
Translation	MMDPLADMRASRAERSGRGAAGGESAEDQTRGCGKNAAMGGSDSLSSFADMDDSLEDKLKGLAFRKQISYRKAISRSGLQHLGPAQNPFPALSNGPAKELRNTVDWSENAVNGDHLWLETNCSGELCYLGEETCVAKIAKSAPRRKCAACKIVVHTACIEELDKINFRCKPTFREGSSRCLRDQNVLRHHWVHRRRQEGKCRQCGKSFPQKFFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVRKPQSSFKNSTRRKKRTSFKRRASKKGTDESKWRPFMLKPLPSPLMKPVLVFVNPKSGGNQGTKLLQMFMWILNPRQVFDLSQGGPRDALELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGSVVQLDRWNLQVERSVVQHEEGTQKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVRVVCDGTDLTAKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLAALQVGGHGDRLHQCREVLITTFKTLPVQVDGEPCRLAPSTLRISLRNQANMVQKSKRRTSVPLLNDPHAVPERLRLRVNRIGLQDYDSMQFDKERLRDISTAVGIVVVRGDCDLETCRLYIDRLQEDLHQAPSAGHRVHYQDECAGLPRPSSAHRLSANWSFLDSTSADRFYRIDKAQEHLHFVTEICQDEVYILDHEAPAVSQVRAPGMPDVVVEPSSGVPLTSDEQALLSAAAKGDLTAVSSSCRSGLSLLVRDAMGCTALHLAAHHGHADVVSFILEHGSKLMLDLTERDTGDTALHKAAAQQQRDVCKRLLEAGASPHKTNFLGKTPKDCALDAGNLELASMLEIQAVDEPASHEDLETAV

Target ORF information:

RefSeq Version	XM_002666976.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666976.5

ORF Insert Sequence:

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACC AGAATGTGTT GCGGCACCAC TGGGTTCATC GTCGCCGACA GGAGGGAAAA 
TGTCGACAGT GCGGCAAAAG TTTTCCACAG AAGTTCTTCC ACAGTAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA ACAGGCGTTC CACAACAAGG TGACGTGTTT CATGCTGCAT 
CAGATTGAGG AGCCCTGTTC ACTGGGAGCT CACGCCGGGG TCATTGTCCC ACCATCCTGG 
ATTATTAAAG TCCGGAAACC GCAGAGCTCA TTTAAAAACT CCACTCGGAG GAAAAAACGG 
ACGTCGTTCA AAAGAAGAGC CAGCAAAAAG GGCACGGATG AATCTAAATG GCGTCCATTC 
ATGCTGAAGC CGCTGCCGTC ACCGCTGATG AAACCAGTGC TGGTGTTCGT CAACCCGAAA 
AGTGGAGGAA ACCAGGGCAC GAAGCTCCTG CAGATGTTCA TGTGGATCCT GAACCCTCGG 
CAGGTGTTTG ATTTGTCTCA GGGAGGCCCC AGAGATGCGT TGGAGTTGTA CAGGAAAGTG 
CCAAATCTTC GCATCCTGGC CTGTGGAGGA GATGGAACCG TGGGCTGGAT TCTGTCGGCT 
TTAGATGAGC TTCAGATGAA CCCTCAGCCT CCTGTGGCGG TACTTCCTCT TGGAACAGGA 
AATGACCTTG CTAGGACACT CAACTGGGGA GGTGGCTACA CAGACGAGCC GGTGTCGAAG 
GTGTTGTGTC ATGTGGAGGA CGGCTCTGTG GTCCAGCTGG ACCGCTGGAA TCTGCAGGTG 
GAGCGCTCAG TGGTCCAGCA CGAGGAGGGA ACACAGAAGC TTCCTTTAAA TGTGTTCAAT 
AACTACTTCA GCCTCGGTTT CGATGCCCAC GTCACCCTGG AGTTTCACGA GTCCAGAGAA 
GCAAACCCAG AGAAATTCAA CAGTCGCTTC CGTAACAAGA TGTTCTATGC AGGGGCTGCG 
TTCTCAGACT TCCTCCAGAG GAGCTCCAGG GATCTGTCTA AGCATGTCCG AGTTGTGTGT 
GACGGCACCG ATCTCACAGC CAAGATCCAG GAGCTCAAGT TTCAGTGCAT CGTCTTCTTG 
AATATTCCCA GATACTGTGC TGGAACAATG CCATGGGGAA ACACAGGAGA CCACCGAGAC 
TTTGAGCCAC AGCGACATGA TGATGGCTGC ATCGAGGTCA TCGGCTTCAC CATGGCCTCT 
CTGGCGGCGC TGCAGGTTGG TGGTCATGGG GATCGTCTCC ATCAGTGTCG AGAGGTGCTC 
ATCACCACCT TCAAGACTCT GCCAGTGCAG GTAGATGGAG AGCCGTGCCG TCTGGCGCCC 
TCCACCCTGC GCATCTCTCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGAGACGC 
ACATCTGTAC CGCTGCTCAA TGACCCTCAC GCCGTTCCAG AGCGCCTGCG TCTGCGTGTC 
AACCGCATCG GTCTGCAGGA CTACGACAGC ATGCAGTTCG ATAAGGAACG TCTGCGCGAC 
ATCTCGACTG CTGTTGGCAT CGTGGTGGTG AGAGGAGACT GTGACCTCGA AACCTGTCGA 
CTCTACATTG ATAGACTGCA GGAGGATTTG CATCAGGCGC CCTCTGCTGG CCACCGAGTG 
CACTATCAGG ATGAGTGTGC AGGTTTGCCA CGGCCGAGTT CAGCACACAG ACTTTCAGCC 
AACTGGAGCT TCCTGGACTC TACATCAGCT GATCGCTTTT ATCGCATCGA CAAGGCTCAG 
GAGCATCTTC ACTTTGTAAC TGAAATATGC CAGGATGAGG TTTATATCCT GGACCATGAA 
GCCCCAGCGG TGAGCCAGGT CAGGGCTCCA GGAATGCCTG ATGTGGTGGT GGAGCCCAGT 
TCTGGTGTCC CTCTGACATC AGATGAGCAA GCTCTGCTGT CGGCGGCTGC GAAAGGGGAC 
TTGACTGCGG TGTCATCGTC CTGCAGGAGT GGGCTTAGTC TGCTGGTGCG GGACGCTATG 
GGCTGCACCG CTCTGCATTT GGCTGCACAT CACGGACACG CAGACGTGGT GAGCTTCATT 
CTGGAGCATG GGTCAAAGCT GATGTTGGAT TTAACAGAGC GAGACACAGG TGACACAGCA 
TTGCACAAAG CGGCGGCACA ACAACAGCGT GATGTTTGCA AACGTCTGCT TGAAGCCGGA 
GCTTCCCCCC ACAAAACCAA CTTCCTGGGG AAAACACCTA AGGATTGTGC CCTGGATGCC 
GGGAATTTAG AGTTGGCGTC CATGCTGGAG ATCCAGGCGG TGGATGAACC AGCATCACAC 
GAGGATCTGG AGACGGCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_009300037.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298312.1 Danio rerio diacylglycerol kinase, iota (dgki), mRNA.

Price & Availability↑

CloneID	ODa49499
Clone ID Related Accession (Same CDS sequence)	XM_009300037.3 , XM_009300037.2
Accession Version	XM_009300037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	diacylglycerol kinase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) and transcript sequence (GFIL01008567.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 404 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACC AGAATGTGTT GCGGCACCAC TGGGTTCATC GTCGCCGACA GGAGGGAAAA 
TGTCGACAGT GCGGCAAAAG TTTTCCACAG AAGTTCTTCC ACAGTAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA ACAGGCGTTC CACAACAAGG TGACGTGTTT CATGCTGCAT 
CAGATTGAGG AGCCCTGTTC ACTGGGAGCT CACGCCGGGG TCATTGTCCC ACCATCCTGG 
ATTATTAAAG TCCGGAAACC GCAGAGCTCA TTTAAAAACT CCACTCGGAG GAAAAAACGG 
ACGTCGTTCA AAAGAAGAGC CAGCAAAAAG GGCACGGATG AATCTAAATG GCGTCCATTC 
ATGCTGAAGC CGCTGCCGTC ACCGCTGATG AAACCAGTGC TGGTGTTCGT CAACCCGAAA 
AGTGGAGGAA ACCAGGGCAC GAAGCTCCTG CAGATGTTCA TGTGGATCCT GAACCCTCGG 
CAGGTGTTTG ATTTGTCTCA GGGAGGCCCC AGAGATGCGT TGGAGTTGTA CAGGAAAGTG 
CCAAATCTTC GCATCCTGGC CTGTGGAGGA GATGGAACCG TGGGCTGGAT TCTGTCGGCT 
TTAGATGAGC TTCAGATGAA CCCTCAGCCT CCTGTGGCGG TACTTCCTCT TGGAACAGGA 
AATGACCTTG CTAGGACACT CAACTGGGGA GGTGGCTACA CAGACGAGCC GGTGTCGAAG 
GTGTTGTGTC ATGTGGAGGA CGGCTCTGTG GTCCAGCTGG ACCGCTGGAA TCTGCAGGTG 
GAGCGCTCAG TGGTCCAGCA CGAGGAGGGA ACACAGAAGC TTCCTTTAAA TGTGTTCAAT 
AACTACTTCA GCCTCGGTTT CGATGCCCAC GTCACCCTGG AGTTTCACGA GTCCAGAGAA 
GCAAACCCAG AGAAATTCAA CAGTCGCTTC CGTAACAAGA TGTTCTATGC AGGGGCTGCG 
TTCTCAGACT TCCTCCAGAG GAGCTCCAGG GATCTGTCTA AGCATGTCCG AGTTGTGTGT 
GACGGCACCG ATCTCACAGC CAAGATCCAG GAGCTCAAGT TTCAGTGCAT CGTCTTCTTG 
AATATTCCCA GATACTGTGC TGGAACAATG CCATGGGGAA ACACAGGAGA CCACCGAGAC 
TTTGAGCCAC AGCGACATGA TGATGGCTGC ATCGAGGTCA TCGGCTTCAC CATGGCCTCT 
CTGGCGGCGC TGCAGGTTGG TGGTCATGGG GATCGTCTCC ATCAGTGTCG AGAGGTGCTC 
ATCACCACCT TCAAGACTCT GCCAGTGCAG GTAGATGGAG AGCCGTGCCG TCTGGCGCCC 
TCCACCCTGC GCATCTCTCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGAGACGC 
ACATCTGTAC CGCTGCTCAA TGACATTCAG AAAGTGTGTG CAGCCGACCT GCGCCGCCTC 
TCTGCTCCCC CAGACTCCTT CTCTGTCCCT CACGCCGTTC CAGAGCGCCT GCGTCTGCGT 
GTCAACCGCA TCGGTCTGCA GGACTACGAC AGCATGCAGT TCGATAAGGA ACGTCTGCGC 
GACATCTCGA CTGCTGTTGG CATCGTGGTG GTGAGAGGAG ACTGTGACCT CGAAACCTGT 
CGACTCTACA TTGATAGACT GCAGGAGGAT TTGCATCAGG CGCCCTCTGC TGGCCACCGA 
GTGCACTATC AGGATGAGTG TGCAGGTTTG CCACGGCCGA GTTCAGCACA CAGACTTTCA 
GCCAACTGGA GCTTCCTGGA CTCTACATCA GCTGATCGCT TTTATCGCAT CGACAAGGCT 
CAGGAGCATC TTCACTTTGT AACTGAAATA TGCCAGGATG AGGTTTATAT CCTGGACCAT 
GAAGCCCCAG CGGTGAGCCA GGTCAGGGCT CCAGGAATGC CTGATGTGGT GGTGGAGCCC 
AGTTCTGGTG TCCCTCTGAC ATCAGATGAG CAAGCTCTGC TGTCGGCGGC TGCGAAAGGG 
GACTTGACTG CGGTGTCATC GTCCTGCAGG AGTGGGCTTA GTCTGCTGGT GCGGGACGCT 
ATGGGCTGCA CCGCTCTGCA TTTGGCTGCA CATCACGGAC ACGCAGACGT GGTGAGCTTC 
ATTCTGGAGC ATGGGTCAAA GCTGATGTTG GATTTAACAG AGCGAGACAC AGGTGACACA 
GCATTGCACA AAGCGGCGGC ACAACAACAG CGTGATGTTT GCAAACGTCT GCTTGAAGCC 
GGAGCTTCCC CCCACAAAAC CAACTTCCTG GGGAAAACAC CTAAGGATTG TGCCCTGGAT 
GCCGGGAATT TAGAGTTGGC GTCCATGCTG GAGATCCAGG CGGTGGATGA ACCAGCATCA 
CACGAGGATC TGGAGACGGC AGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298312.1
CDS	552..3458
Translation	MMDPLADMRASRAERSGRGAAGGESAEDQTRGCGKNAAMGGSDSLSSFADMDDSLEDKLKGLAFRKQISYRKAISRSGLQHLGPAQNPFPALSNGPAKELRNTVDWSENAVNGDHLWLETNCSGELCYLGEETCVAKIAKSAPRRKCAACKIVVHTACIEELDKINFRCKPTFREGSSRCLRDQNVLRHHWVHRRRQEGKCRQCGKSFPQKFFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVRKPQSSFKNSTRRKKRTSFKRRASKKGTDESKWRPFMLKPLPSPLMKPVLVFVNPKSGGNQGTKLLQMFMWILNPRQVFDLSQGGPRDALELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGSVVQLDRWNLQVERSVVQHEEGTQKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVRVVCDGTDLTAKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLAALQVGGHGDRLHQCREVLITTFKTLPVQVDGEPCRLAPSTLRISLRNQANMVQKSKRRTSVPLLNDIQKVCAADLRRLSAPPDSFSVPHAVPERLRLRVNRIGLQDYDSMQFDKERLRDISTAVGIVVVRGDCDLETCRLYIDRLQEDLHQAPSAGHRVHYQDECAGLPRPSSAHRLSANWSFLDSTSADRFYRIDKAQEHLHFVTEICQDEVYILDHEAPAVSQVRAPGMPDVVVEPSSGVPLTSDEQALLSAAAKGDLTAVSSSCRSGLSLLVRDAMGCTALHLAAHHGHADVVSFILEHGSKLMLDLTERDTGDTALHKAAAQQQRDVCKRLLEAGASPHKTNFLGKTPKDCALDAGNLELASMLEIQAVDEPASHEDLETAV

Target ORF information:

RefSeq Version	XM_009300037.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio diacylglycerol kinase, iota (dgki), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300037.3

ORF Insert Sequence:

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACC AGAATGTGTT GCGGCACCAC TGGGTTCATC GTCGCCGACA GGAGGGAAAA 
TGTCGACAGT GCGGCAAAAG TTTTCCACAG AAGTTCTTCC ACAGTAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA ACAGGCGTTC CACAACAAGG TGACGTGTTT CATGCTGCAT 
CAGATTGAGG AGCCCTGTTC ACTGGGAGCT CACGCCGGGG TCATTGTCCC ACCATCCTGG 
ATTATTAAAG TCCGGAAACC GCAGAGCTCA TTTAAAAACT CCACTCGGAG GAAAAAACGG 
ACGTCGTTCA AAAGAAGAGC CAGCAAAAAG GGCACGGATG AATCTAAATG GCGTCCATTC 
ATGCTGAAGC CGCTGCCGTC ACCGCTGATG AAACCAGTGC TGGTGTTCGT CAACCCGAAA 
AGTGGAGGAA ACCAGGGCAC GAAGCTCCTG CAGATGTTCA TGTGGATCCT GAACCCTCGG 
CAGGTGTTTG ATTTGTCTCA GGGAGGCCCC AGAGATGCGT TGGAGTTGTA CAGGAAAGTG 
CCAAATCTTC GCATCCTGGC CTGTGGAGGA GATGGAACCG TGGGCTGGAT TCTGTCGGCT 
TTAGATGAGC TTCAGATGAA CCCTCAGCCT CCTGTGGCGG TACTTCCTCT TGGAACAGGA 
AATGACCTTG CTAGGACACT CAACTGGGGA GGTGGCTACA CAGACGAGCC GGTGTCGAAG 
GTGTTGTGTC ATGTGGAGGA CGGCTCTGTG GTCCAGCTGG ACCGCTGGAA TCTGCAGGTG 
GAGCGCTCAG TGGTCCAGCA CGAGGAGGGA ACACAGAAGC TTCCTTTAAA TGTGTTCAAT 
AACTACTTCA GCCTCGGTTT CGATGCCCAC GTCACCCTGG AGTTTCACGA GTCCAGAGAA 
GCAAACCCAG AGAAATTCAA CAGTCGCTTC CGTAACAAGA TGTTCTATGC AGGGGCTGCG 
TTCTCAGACT TCCTCCAGAG GAGCTCCAGG GATCTGTCTA AGCATGTCCG AGTTGTGTGT 
GACGGCACCG ATCTCACAGC CAAGATCCAG GAGCTCAAGT TTCAGTGCAT CGTCTTCTTG 
AATATTCCCA GATACTGTGC TGGAACAATG CCATGGGGAA ACACAGGAGA CCACCGAGAC 
TTTGAGCCAC AGCGACATGA TGATGGCTGC ATCGAGGTCA TCGGCTTCAC CATGGCCTCT 
CTGGCGGCGC TGCAGGTTGG TGGTCATGGG GATCGTCTCC ATCAGTGTCG AGAGGTGCTC 
ATCACCACCT TCAAGACTCT GCCAGTGCAG GTAGATGGAG AGCCGTGCCG TCTGGCGCCC 
TCCACCCTGC GCATCTCTCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGAGACGC 
ACATCTGTAC CGCTGCTCAA TGACATTCAG AAAGTGTGTG CAGCCGACCT GCGCCGCCTC 
TCTGCTCCCC CAGACTCCTT CTCTGTCCCT CACGCCGTTC CAGAGCGCCT GCGTCTGCGT 
GTCAACCGCA TCGGTCTGCA GGACTACGAC AGCATGCAGT TCGATAAGGA ACGTCTGCGC 
GACATCTCGA CTGCTGTTGG CATCGTGGTG GTGAGAGGAG ACTGTGACCT CGAAACCTGT 
CGACTCTACA TTGATAGACT GCAGGAGGAT TTGCATCAGG CGCCCTCTGC TGGCCACCGA 
GTGCACTATC AGGATGAGTG TGCAGGTTTG CCACGGCCGA GTTCAGCACA CAGACTTTCA 
GCCAACTGGA GCTTCCTGGA CTCTACATCA GCTGATCGCT TTTATCGCAT CGACAAGGCT 
CAGGAGCATC TTCACTTTGT AACTGAAATA TGCCAGGATG AGGTTTATAT CCTGGACCAT 
GAAGCCCCAG CGGTGAGCCA GGTCAGGGCT CCAGGAATGC CTGATGTGGT GGTGGAGCCC 
AGTTCTGGTG TCCCTCTGAC ATCAGATGAG CAAGCTCTGC TGTCGGCGGC TGCGAAAGGG 
GACTTGACTG CGGTGTCATC GTCCTGCAGG AGTGGGCTTA GTCTGCTGGT GCGGGACGCT 
ATGGGCTGCA CCGCTCTGCA TTTGGCTGCA CATCACGGAC ACGCAGACGT GGTGAGCTTC 
ATTCTGGAGC ATGGGTCAAA GCTGATGTTG GATTTAACAG AGCGAGACAC AGGTGACACA 
GCATTGCACA AAGCGGCGGC ACAACAACAG CGTGATGTTT GCAAACGTCT GCTTGAAGCC 
GGAGCTTCCC CCCACAAAAC CAACTTCCTG GGGAAAACAC CTAAGGATTG TGCCCTGGAT 
GCCGGGAATT TAGAGTTGGC GTCCATGCTG GAGATCCAGG CGGTGGATGA ACCAGCATCA 
CACGAGGATC TGGAGACGGC AGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_009300037.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298312.1 Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa49499
Clone ID Related Accession (Same CDS sequence)	XM_009300037.3 , XM_009300037.2
Accession Version	XM_009300037.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase iota isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACC AGAATGTGTT GCGGCACCAC TGGGTTCATC GTCGCCGACA GGAGGGAAAA 
TGTCGACAGT GCGGCAAAAG TTTTCCACAG AAGTTCTTCC ACAGTAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA ACAGGCGTTC CACAACAAGG TGACGTGTTT CATGCTGCAT 
CAGATTGAGG AGCCCTGTTC ACTGGGAGCT CACGCCGGGG TCATTGTCCC ACCATCCTGG 
ATTATTAAAG TCCGGAAACC GCAGAGCTCA TTTAAAAACT CCACTCGGAG GAAAAAACGG 
ACGTCGTTCA AAAGAAGAGC CAGCAAAAAG GGCACGGATG AATCTAAATG GCGTCCATTC 
ATGCTGAAGC CGCTGCCGTC ACCGCTGATG AAACCAGTGC TGGTGTTCGT CAACCCGAAA 
AGTGGAGGAA ACCAGGGCAC GAAGCTCCTG CAGATGTTCA TGTGGATCCT GAACCCTCGG 
CAGGTGTTTG ATTTGTCTCA GGGAGGCCCC AGAGATGCGT TGGAGTTGTA CAGGAAAGTG 
CCAAATCTTC GCATCCTGGC CTGTGGAGGA GATGGAACCG TGGGCTGGAT TCTGTCGGCT 
TTAGATGAGC TTCAGATGAA CCCTCAGCCT CCTGTGGCGG TACTTCCTCT TGGAACAGGA 
AATGACCTTG CTAGGACACT CAACTGGGGA GGTGGCTACA CAGACGAGCC GGTGTCGAAG 
GTGTTGTGTC ATGTGGAGGA CGGCTCTGTG GTCCAGCTGG ACCGCTGGAA TCTGCAGGTG 
GAGCGCTCAG TGGTCCAGCA CGAGGAGGGA ACACAGAAGC TTCCTTTAAA TGTGTTCAAT 
AACTACTTCA GCCTCGGTTT CGATGCCCAC GTCACCCTGG AGTTTCACGA GTCCAGAGAA 
GCAAACCCAG AGAAATTCAA CAGTCGCTTC CGTAACAAGA TGTTCTATGC AGGGGCTGCG 
TTCTCAGACT TCCTCCAGAG GAGCTCCAGG GATCTGTCTA AGCATGTCCG AGTTGTGTGT 
GACGGCACCG ATCTCACAGC CAAGATCCAG GAGCTCAAGT TTCAGTGCAT CGTCTTCTTG 
AATATTCCCA GATACTGTGC TGGAACAATG CCATGGGGAA ACACAGGAGA CCACCGAGAC 
TTTGAGCCAC AGCGACATGA TGATGGCTGC ATCGAGGTCA TCGGCTTCAC CATGGCCTCT 
CTGGCGGCGC TGCAGGTTGG TGGTCATGGG GATCGTCTCC ATCAGTGTCG AGAGGTGCTC 
ATCACCACCT TCAAGACTCT GCCAGTGCAG GTAGATGGAG AGCCGTGCCG TCTGGCGCCC 
TCCACCCTGC GCATCTCTCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGAGACGC 
ACATCTGTAC CGCTGCTCAA TGACATTCAG AAAGTGTGTG CAGCCGACCT GCGCCGCCTC 
TCTGCTCCCC CAGACTCCTT CTCTGTCCCT CACGCCGTTC CAGAGCGCCT GCGTCTGCGT 
GTCAACCGCA TCGGTCTGCA GGACTACGAC AGCATGCAGT TCGATAAGGA ACGTCTGCGC 
GACATCTCGA CTGCTGTTGG CATCGTGGTG GTGAGAGGAG ACTGTGACCT CGAAACCTGT 
CGACTCTACA TTGATAGACT GCAGGAGGAT TTGCATCAGG CGCCCTCTGC TGGCCACCGA 
GTGCACTATC AGGATGAGTG TGCAGGTTTG CCACGGCCGA GTTCAGCACA CAGACTTTCA 
GCCAACTGGA GCTTCCTGGA CTCTACATCA GCTGATCGCT TTTATCGCAT CGACAAGGCT 
CAGGAGCATC TTCACTTTGT AACTGAAATA TGCCAGGATG AGGTTTATAT CCTGGACCAT 
GAAGCCCCAG CGGTGAGCCA GGTCAGGGCT CCAGGAATGC CTGATGTGGT GGTGGAGCCC 
AGTTCTGGTG TCCCTCTGAC ATCAGATGAG CAAGCTCTGC TGTCGGCGGC TGCGAAAGGG 
GACTTGACTG CGGTGTCATC GTCCTGCAGG AGTGGGCTTA GTCTGCTGGT GCGGGACGCT 
ATGGGCTGCA CCGCTCTGCA TTTGGCTGCA CATCACGGAC ACGCAGACGT GGTGAGCTTC 
ATTCTGGAGC ATGGGTCAAA GCTGATGTTG GATTTAACAG AGCGAGACAC AGGTGACACA 
GCATTGCACA AAGCGGCGGC ACAACAACAG CGTGATGTTT GCAAACGTCT GCTTGAAGCC 
GGAGCTTCCC CCCACAAAAC CAACTTCCTG GGGAAAACAC CTAAGGATTG TGCCCTGGAT 
GCCGGGAATT TAGAGTTGGC GTCCATGCTG GAGATCCAGG CGGTGGATGA ACCAGCATCA 
CACGAGGATC TGGAGACGGC AGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298312.1
CDS	554..3460
Translation	MMDPLADMRASRAERSGRGAAGGESAEDQTRGCGKNAAMGGSDSLSSFADMDDSLEDKLKGLAFRKQISYRKAISRSGLQHLGPAQNPFPALSNGPAKELRNTVDWSENAVNGDHLWLETNCSGELCYLGEETCVAKIAKSAPRRKCAACKIVVHTACIEELDKINFRCKPTFREGSSRCLRDQNVLRHHWVHRRRQEGKCRQCGKSFPQKFFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVRKPQSSFKNSTRRKKRTSFKRRASKKGTDESKWRPFMLKPLPSPLMKPVLVFVNPKSGGNQGTKLLQMFMWILNPRQVFDLSQGGPRDALELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGSVVQLDRWNLQVERSVVQHEEGTQKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVRVVCDGTDLTAKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLAALQVGGHGDRLHQCREVLITTFKTLPVQVDGEPCRLAPSTLRISLRNQANMVQKSKRRTSVPLLNDIQKVCAADLRRLSAPPDSFSVPHAVPERLRLRVNRIGLQDYDSMQFDKERLRDISTAVGIVVVRGDCDLETCRLYIDRLQEDLHQAPSAGHRVHYQDECAGLPRPSSAHRLSANWSFLDSTSADRFYRIDKAQEHLHFVTEICQDEVYILDHEAPAVSQVRAPGMPDVVVEPSSGVPLTSDEQALLSAAAKGDLTAVSSSCRSGLSLLVRDAMGCTALHLAAHHGHADVVSFILEHGSKLMLDLTERDTGDTALHKAAAQQQRDVCKRLLEAGASPHKTNFLGKTPKDCALDAGNLELASMLEIQAVDEPASHEDLETAV

Target ORF information:

RefSeq Version	XM_009300037.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300037.2

ORF Insert Sequence:

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACC AGAATGTGTT GCGGCACCAC TGGGTTCATC GTCGCCGACA GGAGGGAAAA 
TGTCGACAGT GCGGCAAAAG TTTTCCACAG AAGTTCTTCC ACAGTAAGGA GATCGTGGCC 
ATCAGCTGCT CCTGGTGCAA ACAGGCGTTC CACAACAAGG TGACGTGTTT CATGCTGCAT 
CAGATTGAGG AGCCCTGTTC ACTGGGAGCT CACGCCGGGG TCATTGTCCC ACCATCCTGG 
ATTATTAAAG TCCGGAAACC GCAGAGCTCA TTTAAAAACT CCACTCGGAG GAAAAAACGG 
ACGTCGTTCA AAAGAAGAGC CAGCAAAAAG GGCACGGATG AATCTAAATG GCGTCCATTC 
ATGCTGAAGC CGCTGCCGTC ACCGCTGATG AAACCAGTGC TGGTGTTCGT CAACCCGAAA 
AGTGGAGGAA ACCAGGGCAC GAAGCTCCTG CAGATGTTCA TGTGGATCCT GAACCCTCGG 
CAGGTGTTTG ATTTGTCTCA GGGAGGCCCC AGAGATGCGT TGGAGTTGTA CAGGAAAGTG 
CCAAATCTTC GCATCCTGGC CTGTGGAGGA GATGGAACCG TGGGCTGGAT TCTGTCGGCT 
TTAGATGAGC TTCAGATGAA CCCTCAGCCT CCTGTGGCGG TACTTCCTCT TGGAACAGGA 
AATGACCTTG CTAGGACACT CAACTGGGGA GGTGGCTACA CAGACGAGCC GGTGTCGAAG 
GTGTTGTGTC ATGTGGAGGA CGGCTCTGTG GTCCAGCTGG ACCGCTGGAA TCTGCAGGTG 
GAGCGCTCAG TGGTCCAGCA CGAGGAGGGA ACACAGAAGC TTCCTTTAAA TGTGTTCAAT 
AACTACTTCA GCCTCGGTTT CGATGCCCAC GTCACCCTGG AGTTTCACGA GTCCAGAGAA 
GCAAACCCAG AGAAATTCAA CAGTCGCTTC CGTAACAAGA TGTTCTATGC AGGGGCTGCG 
TTCTCAGACT TCCTCCAGAG GAGCTCCAGG GATCTGTCTA AGCATGTCCG AGTTGTGTGT 
GACGGCACCG ATCTCACAGC CAAGATCCAG GAGCTCAAGT TTCAGTGCAT CGTCTTCTTG 
AATATTCCCA GATACTGTGC TGGAACAATG CCATGGGGAA ACACAGGAGA CCACCGAGAC 
TTTGAGCCAC AGCGACATGA TGATGGCTGC ATCGAGGTCA TCGGCTTCAC CATGGCCTCT 
CTGGCGGCGC TGCAGGTTGG TGGTCATGGG GATCGTCTCC ATCAGTGTCG AGAGGTGCTC 
ATCACCACCT TCAAGACTCT GCCAGTGCAG GTAGATGGAG AGCCGTGCCG TCTGGCGCCC 
TCCACCCTGC GCATCTCTCT GAGGAACCAG GCCAACATGG TGCAGAAGAG CAAGAGACGC 
ACATCTGTAC CGCTGCTCAA TGACATTCAG AAAGTGTGTG CAGCCGACCT GCGCCGCCTC 
TCTGCTCCCC CAGACTCCTT CTCTGTCCCT CACGCCGTTC CAGAGCGCCT GCGTCTGCGT 
GTCAACCGCA TCGGTCTGCA GGACTACGAC AGCATGCAGT TCGATAAGGA ACGTCTGCGC 
GACATCTCGA CTGCTGTTGG CATCGTGGTG GTGAGAGGAG ACTGTGACCT CGAAACCTGT 
CGACTCTACA TTGATAGACT GCAGGAGGAT TTGCATCAGG CGCCCTCTGC TGGCCACCGA 
GTGCACTATC AGGATGAGTG TGCAGGTTTG CCACGGCCGA GTTCAGCACA CAGACTTTCA 
GCCAACTGGA GCTTCCTGGA CTCTACATCA GCTGATCGCT TTTATCGCAT CGACAAGGCT 
CAGGAGCATC TTCACTTTGT AACTGAAATA TGCCAGGATG AGGTTTATAT CCTGGACCAT 
GAAGCCCCAG CGGTGAGCCA GGTCAGGGCT CCAGGAATGC CTGATGTGGT GGTGGAGCCC 
AGTTCTGGTG TCCCTCTGAC ATCAGATGAG CAAGCTCTGC TGTCGGCGGC TGCGAAAGGG 
GACTTGACTG CGGTGTCATC GTCCTGCAGG AGTGGGCTTA GTCTGCTGGT GCGGGACGCT 
ATGGGCTGCA CCGCTCTGCA TTTGGCTGCA CATCACGGAC ACGCAGACGT GGTGAGCTTC 
ATTCTGGAGC ATGGGTCAAA GCTGATGTTG GATTTAACAG AGCGAGACAC AGGTGACACA 
GCATTGCACA AAGCGGCGGC ACAACAACAG CGTGATGTTT GCAAACGTCT GCTTGAAGCC 
GGAGCTTCCC CCCACAAAAC CAACTTCCTG GGGAAAACAC CTAAGGATTG TGCCCTGGAT 
GCCGGGAATT TAGAGTTGGC GTCCATGCTG GAGATCCAGG CGGTGGATGA ACCAGCATCA 
CACGAGGATC TGGAGACGGC AGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_009300038.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298313.1 Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa49500
Clone ID Related Accession (Same CDS sequence)	XM_009300038.2
Accession Version	XM_009300038.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase iota isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACA ATGTGTTGCG GCACCACTGG GTTCATCGTC GCCGACAGGA GGGAAAATGT 
CGACAGTGCG GCAAAAGTTT TCCACAGAAG TTCTTCCACA GTAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAACA GGCGTTCCAC AACAAGGTGA CGTGTTTCAT GCTGCATCAG 
ATTGAGGAGC CCTGTTCACT GGGAGCTCAC GCCGGGGTCA TTGTCCCACC ATCCTGGATT 
ATTAAAGTCC GGAAACCGCA GAGCTCATTT AAAAACTCCA CTCGGAGGAA AAAACGGACG 
TCGTTCAAAA GAAGAGCCAG CAAAAAGGGC ACGGATGAAT CTAAATGGCG TCCATTCATG 
CTGAAGCCGC TGCCGTCACC GCTGATGAAA CCAGTGCTGG TGTTCGTCAA CCCGAAAAGT 
GGAGGAAACC AGGGCACGAA GCTCCTGCAG ATGTTCATGT GGATCCTGAA CCCTCGGCAG 
GTGTTTGATT TGTCTCAGGG AGGCCCCAGA GATGCGTTGG AGTTGTACAG GAAAGTGCCA 
AATCTTCGCA TCCTGGCCTG TGGAGGAGAT GGAACCGTGG GCTGGATTCT GTCGGCTTTA 
GATGAGCTTC AGATGAACCC TCAGCCTCCT GTGGCGGTAC TTCCTCTTGG AACAGGAAAT 
GACCTTGCTA GGACACTCAA CTGGGGAGGT GGCTACACAG ACGAGCCGGT GTCGAAGGTG 
TTGTGTCATG TGGAGGACGG CTCTGTGGTC CAGCTGGACC GCTGGAATCT GCAGGTGGAG 
CGCTCAGTGG TCCAGCACGA GGAGGGAACA CAGAAGCTTC CTTTAAATGT GTTCAATAAC 
TACTTCAGCC TCGGTTTCGA TGCCCACGTC ACCCTGGAGT TTCACGAGTC CAGAGAAGCA 
AACCCAGAGA AATTCAACAG TCGCTTCCGT AACAAGATGT TCTATGCAGG GGCTGCGTTC 
TCAGACTTCC TCCAGAGGAG CTCCAGGGAT CTGTCTAAGC ATGTCCGAGT TGTGTGTGAC 
GGCACCGATC TCACAGCCAA GATCCAGGAG CTCAAGTTTC AGTGCATCGT CTTCTTGAAT 
ATTCCCAGAT ACTGTGCTGG AACAATGCCA TGGGGAAACA CAGGAGACCA CCGAGACTTT 
GAGCCACAGC GACATGATGA TGGCTGCATC GAGGTCATCG GCTTCACCAT GGCCTCTCTG 
GCGGCGCTGC AGGTTGGTGG TCATGGGGAT CGTCTCCATC AGTGTCGAGA GGTGCTCATC 
ACCACCTTCA AGACTCTGCC AGTGCAGGTA GATGGAGAGC CGTGCCGTCT GGCGCCCTCC 
ACCCTGCGCA TCTCTCTGAG GAACCAGGCC AACATGGTGC AGAAGAGCAA GAGACGCACA 
TCTGTACCGC TGCTCAATGA CATTCAGAAA GTGTGTGCAG CCGACCTGCG CCGCCTCTCT 
GCTCCCCCAG ACTCCTTCTC TGTCCCTCAC GCCGTTCCAG AGCGCCTGCG TCTGCGTGTC 
AACCGCATCG GTCTGCAGGA CTACGACAGC ATGCAGTTCG ATAAGGAACG TCTGCGCGAC 
ATCTCGACTG CTGTTGGCAT CGTGGTGGTG AGAGGAGACT GTGACCTCGA AACCTGTCGA 
CTCTACATTG ATAGACTGCA GGAGGATTTG CATCAGGCGC CCTCTGCTGG CCACCGAGTG 
CACTATCAGG ATGAGTGTGC AGGTTTGCCA CGGCCGAGTT CAGCACACAG ACTTTCAGCC 
AACTGGAGCT TCCTGGACTC TACATCAGCT GATCGCTTTT ATCGCATCGA CAAGGCTCAG 
GAGCATCTTC ACTTTGTAAC TGAAATATGC CAGGATGAGG TTTATATCCT GGACCATGAA 
GCCCCAGCGG TGAGCCAGGT CAGGGCTCCA GGAATGCCTG ATGTGGTGGT GGAGCCCAGT 
TCTGGTGTCC CTCTGACATC AGATGAGCAA GCTCTGCTGT CGGCGGCTGC GAAAGGGGAC 
TTGACTGCGG TGTCATCGTC CTGCAGGAGT GGGCTTAGTC TGCTGGTGCG GGACGCTATG 
GGCTGCACCG CTCTGCATTT GGCTGCACAT CACGGACACG CAGACGTGGT GAGCTTCATT 
CTGGAGCATG GGTCAAAGCT GATGTTGGAT TTAACAGAGC GAGACACAGG TGACACAGCA 
TTGCACAAAG CGGCGGCACA ACAACAGCGT GATGTTTGCA AACGTCTGCT TGAAGCCGGA 
GCTTCCCCCC ACAAAACCAA CTTCCTGGGG AAAACACCTA AGGATTGTGC CCTGGATGCC 
GGGAATTTAG AGTTGGCGTC CATGCTGGAG ATCCAGGCGG TGGATGAACC AGCATCACAC 
GAGGATCTGG AGACGGCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298313.1
CDS	554..3457
Translation	MMDPLADMRASRAERSGRGAAGGESAEDQTRGCGKNAAMGGSDSLSSFADMDDSLEDKLKGLAFRKQISYRKAISRSGLQHLGPAQNPFPALSNGPAKELRNTVDWSENAVNGDHLWLETNCSGELCYLGEETCVAKIAKSAPRRKCAACKIVVHTACIEELDKINFRCKPTFREGSSRCLRDNVLRHHWVHRRRQEGKCRQCGKSFPQKFFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVRKPQSSFKNSTRRKKRTSFKRRASKKGTDESKWRPFMLKPLPSPLMKPVLVFVNPKSGGNQGTKLLQMFMWILNPRQVFDLSQGGPRDALELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGSVVQLDRWNLQVERSVVQHEEGTQKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVRVVCDGTDLTAKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLAALQVGGHGDRLHQCREVLITTFKTLPVQVDGEPCRLAPSTLRISLRNQANMVQKSKRRTSVPLLNDIQKVCAADLRRLSAPPDSFSVPHAVPERLRLRVNRIGLQDYDSMQFDKERLRDISTAVGIVVVRGDCDLETCRLYIDRLQEDLHQAPSAGHRVHYQDECAGLPRPSSAHRLSANWSFLDSTSADRFYRIDKAQEHLHFVTEICQDEVYILDHEAPAVSQVRAPGMPDVVVEPSSGVPLTSDEQALLSAAAKGDLTAVSSSCRSGLSLLVRDAMGCTALHLAAHHGHADVVSFILEHGSKLMLDLTERDTGDTALHKAAAQQQRDVCKRLLEAGASPHKTNFLGKTPKDCALDAGNLELASMLEIQAVDEPASHEDLETAV

Target ORF information:

RefSeq Version	XM_009300038.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300038.2

ORF Insert Sequence:

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACA ATGTGTTGCG GCACCACTGG GTTCATCGTC GCCGACAGGA GGGAAAATGT 
CGACAGTGCG GCAAAAGTTT TCCACAGAAG TTCTTCCACA GTAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAACA GGCGTTCCAC AACAAGGTGA CGTGTTTCAT GCTGCATCAG 
ATTGAGGAGC CCTGTTCACT GGGAGCTCAC GCCGGGGTCA TTGTCCCACC ATCCTGGATT 
ATTAAAGTCC GGAAACCGCA GAGCTCATTT AAAAACTCCA CTCGGAGGAA AAAACGGACG 
TCGTTCAAAA GAAGAGCCAG CAAAAAGGGC ACGGATGAAT CTAAATGGCG TCCATTCATG 
CTGAAGCCGC TGCCGTCACC GCTGATGAAA CCAGTGCTGG TGTTCGTCAA CCCGAAAAGT 
GGAGGAAACC AGGGCACGAA GCTCCTGCAG ATGTTCATGT GGATCCTGAA CCCTCGGCAG 
GTGTTTGATT TGTCTCAGGG AGGCCCCAGA GATGCGTTGG AGTTGTACAG GAAAGTGCCA 
AATCTTCGCA TCCTGGCCTG TGGAGGAGAT GGAACCGTGG GCTGGATTCT GTCGGCTTTA 
GATGAGCTTC AGATGAACCC TCAGCCTCCT GTGGCGGTAC TTCCTCTTGG AACAGGAAAT 
GACCTTGCTA GGACACTCAA CTGGGGAGGT GGCTACACAG ACGAGCCGGT GTCGAAGGTG 
TTGTGTCATG TGGAGGACGG CTCTGTGGTC CAGCTGGACC GCTGGAATCT GCAGGTGGAG 
CGCTCAGTGG TCCAGCACGA GGAGGGAACA CAGAAGCTTC CTTTAAATGT GTTCAATAAC 
TACTTCAGCC TCGGTTTCGA TGCCCACGTC ACCCTGGAGT TTCACGAGTC CAGAGAAGCA 
AACCCAGAGA AATTCAACAG TCGCTTCCGT AACAAGATGT TCTATGCAGG GGCTGCGTTC 
TCAGACTTCC TCCAGAGGAG CTCCAGGGAT CTGTCTAAGC ATGTCCGAGT TGTGTGTGAC 
GGCACCGATC TCACAGCCAA GATCCAGGAG CTCAAGTTTC AGTGCATCGT CTTCTTGAAT 
ATTCCCAGAT ACTGTGCTGG AACAATGCCA TGGGGAAACA CAGGAGACCA CCGAGACTTT 
GAGCCACAGC GACATGATGA TGGCTGCATC GAGGTCATCG GCTTCACCAT GGCCTCTCTG 
GCGGCGCTGC AGGTTGGTGG TCATGGGGAT CGTCTCCATC AGTGTCGAGA GGTGCTCATC 
ACCACCTTCA AGACTCTGCC AGTGCAGGTA GATGGAGAGC CGTGCCGTCT GGCGCCCTCC 
ACCCTGCGCA TCTCTCTGAG GAACCAGGCC AACATGGTGC AGAAGAGCAA GAGACGCACA 
TCTGTACCGC TGCTCAATGA CATTCAGAAA GTGTGTGCAG CCGACCTGCG CCGCCTCTCT 
GCTCCCCCAG ACTCCTTCTC TGTCCCTCAC GCCGTTCCAG AGCGCCTGCG TCTGCGTGTC 
AACCGCATCG GTCTGCAGGA CTACGACAGC ATGCAGTTCG ATAAGGAACG TCTGCGCGAC 
ATCTCGACTG CTGTTGGCAT CGTGGTGGTG AGAGGAGACT GTGACCTCGA AACCTGTCGA 
CTCTACATTG ATAGACTGCA GGAGGATTTG CATCAGGCGC CCTCTGCTGG CCACCGAGTG 
CACTATCAGG ATGAGTGTGC AGGTTTGCCA CGGCCGAGTT CAGCACACAG ACTTTCAGCC 
AACTGGAGCT TCCTGGACTC TACATCAGCT GATCGCTTTT ATCGCATCGA CAAGGCTCAG 
GAGCATCTTC ACTTTGTAAC TGAAATATGC CAGGATGAGG TTTATATCCT GGACCATGAA 
GCCCCAGCGG TGAGCCAGGT CAGGGCTCCA GGAATGCCTG ATGTGGTGGT GGAGCCCAGT 
TCTGGTGTCC CTCTGACATC AGATGAGCAA GCTCTGCTGT CGGCGGCTGC GAAAGGGGAC 
TTGACTGCGG TGTCATCGTC CTGCAGGAGT GGGCTTAGTC TGCTGGTGCG GGACGCTATG 
GGCTGCACCG CTCTGCATTT GGCTGCACAT CACGGACACG CAGACGTGGT GAGCTTCATT 
CTGGAGCATG GGTCAAAGCT GATGTTGGAT TTAACAGAGC GAGACACAGG TGACACAGCA 
TTGCACAAAG CGGCGGCACA ACAACAGCGT GATGTTTGCA AACGTCTGCT TGAAGCCGGA 
GCTTCCCCCC ACAAAACCAA CTTCCTGGGG AAAACACCTA AGGATTGTGC CCTGGATGCC 
GGGAATTTAG AGTTGGCGTC CATGCTGGAG ATCCAGGCGG TGGATGAACC AGCATCACAC 
GAGGATCTGG AGACGGCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_009300039.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298314.1 Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa49501
Clone ID Related Accession (Same CDS sequence)	XM_009300039.2
Accession Version	XM_009300039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase iota isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCATCT 
GCTCCCCGGA GGAAGTGTGC GGCCTGTAAG ATCGTGGTTC ACACCGCCTG CATCGAGGAG 
CTGGACAAGA TCAACTTCCG CTGTAAACCC ACTTTCAGAG AGGGCAGCTC TCGATGCCTC 
AGAGACCAGA ATGTGTTGCG GCACCACTGG GTTCATCGTC GCCGACAGGA GGGAAAATGT 
CGACAGTGCG GCAAAAGTTT TCCACAGAAG TTCTTCCACA GTAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAACA GGCGTTCCAC AACAAGGTGA CGTGTTTCAT GCTGCATCAG 
ATTGAGGAGC CCTGTTCACT GGGAGCTCAC GCCGGGGTCA TTGTCCCACC ATCCTGGATT 
ATTAAAGTCC GGAAACCGCA GAGCTCATTT AAAAACTCCA CTCGGAGGAA AAAACGGACG 
TCGTTCAAAA GAAGAGCCAG CAAAAAGGGC ACGGATGAAT CTAAATGGCG TCCATTCATG 
CTGAAGCCGC TGCCGTCACC GCTGATGAAA CCAGTGCTGG TGTTCGTCAA CCCGAAAAGT 
GGAGGAAACC AGGGCACGAA GCTCCTGCAG ATGTTCATGT GGATCCTGAA CCCTCGGCAG 
GTGTTTGATT TGTCTCAGGG AGGCCCCAGA GATGCGTTGG AGTTGTACAG GAAAGTGCCA 
AATCTTCGCA TCCTGGCCTG TGGAGGAGAT GGAACCGTGG GCTGGATTCT GTCGGCTTTA 
GATGAGCTTC AGATGAACCC TCAGCCTCCT GTGGCGGTAC TTCCTCTTGG AACAGGAAAT 
GACCTTGCTA GGACACTCAA CTGGGGAGGT GGCTACACAG ACGAGCCGGT GTCGAAGGTG 
TTGTGTCATG TGGAGGACGG CTCTGTGGTC CAGCTGGACC GCTGGAATCT GCAGGTGGAG 
CGCTCAGTGG TCCAGCACGA GGAGGGAACA CAGAAGCTTC CTTTAAATGT GTTCAATAAC 
TACTTCAGCC TCGGTTTCGA TGCCCACGTC ACCCTGGAGT TTCACGAGTC CAGAGAAGCA 
AACCCAGAGA AATTCAACAG TCGCTTCCGT AACAAGATGT TCTATGCAGG GGCTGCGTTC 
TCAGACTTCC TCCAGAGGAG CTCCAGGGAT CTGTCTAAGC ATGTCCGAGT TGTGTGTGAC 
GGCACCGATC TCACAGCCAA GATCCAGGAG CTCAAGTTTC AGTGCATCGT CTTCTTGAAT 
ATTCCCAGAT ACTGTGCTGG AACAATGCCA TGGGGAAACA CAGGAGACCA CCGAGACTTT 
GAGCCACAGC GACATGATGA TGGCTGCATC GAGGTCATCG GCTTCACCAT GGCCTCTCTG 
GCGGCGCTGC AGGTTGGTGG TCATGGGGAT CGTCTCCATC AGTGTCGAGA GGTGCTCATC 
ACCACCTTCA AGACTCTGCC AGTGCAGGTA GATGGAGAGC CGTGCCGTCT GGCGCCCTCC 
ACCCTGCGCA TCTCTCTGAG GAACCAGGCC AACATGGTGC AGAAGAGCAA GAGACGCACA 
TCTGTACCGC TGCTCAATGA CATTCAGAAA GTGTGTGCAG CCGACCTGCG CCGCCTCTCT 
GCTCCCCCAG ACTCCTTCTC TGTCCCTCAC GCCGTTCCAG AGCGCCTGCG TCTGCGTGTC 
AACCGCATCG GTCTGCAGGA CTACGACAGC ATGCAGTTCG ATAAGGAACG TCTGCGCGAC 
ATCTCGACTG CTGTTGGCAT CGTGGTGGTG AGAGGAGACT GTGACCTCGA AACCTGTCGA 
CTCTACATTG ATAGACTGCA GGAGGATTTG CATCAGGCGC CCTCTGCTGG CCACCGAGTG 
CACTATCAGG ATGAGTGTGC AGGTTTGCCA CGGCCGAGTT CAGCACACAG ACTTTCAGCC 
AACTGGAGCT TCCTGGACTC TACATCAGCT GATCGCTTTT ATCGCATCGA CAAGGCTCAG 
GAGCATCTTC ACTTTGTAAC TGAAATATGC CAGGATGAGG TTTATATCCT GGACCATGAA 
GCCCCAGCGG TGAGCCAGGT CAGGGCTCCA GGAATGCCTG ATGTGGTGGT GGAGCCCAGT 
TCTGGTGTCC CTCTGACATC AGATGAGCAA GCTCTGCTGT CGGCGGCTGC GAAAGGGGAC 
TTGACTGCGG TGTCATCGTC CTGCAGGAGT GGGCTTAGTC TGCTGGTGCG GGACGCTATG 
GGCTGCACCG CTCTGCATTT GGCTGCACAT CACGGACACG CAGACGTGGT GAGCTTCATT 
CTGGAGCATG GGTCAAAGCT GATGTTGGAT TTAACAGAGC GAGACACAGG TGACACAGCA 
TTGCACAAAG CGGCGGCACA ACAACAGCGT GATGTTTGCA AACGTCTGCT TGAAGCCGGA 
GCTTCCCCCC ACAAAACCAA CTTCCTGGGG AAAACACCTA AGGATTGTGC CCTGGATGCC 
GGGAATTTAG AGTTGGCGTC CATGCTGGAG ATCCAGGCGG TGGATGAACC AGCATCACAC 
GAGGATCTGG AGACGGCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298314.1
CDS	554..3457
Translation	MMDPLADMRASRAERSGRGAAGGESAEDQTRGCGKNAAMGGSDSLSSFADMDDSLEDKLKGLAFRKQISYRKAISRSGLQHLGPAQNPFPALSNGPAKELRNTVDWSENAVNGDHLWLETNCSGELCYLGEETCVAKIASAPRRKCAACKIVVHTACIEELDKINFRCKPTFREGSSRCLRDQNVLRHHWVHRRRQEGKCRQCGKSFPQKFFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVRKPQSSFKNSTRRKKRTSFKRRASKKGTDESKWRPFMLKPLPSPLMKPVLVFVNPKSGGNQGTKLLQMFMWILNPRQVFDLSQGGPRDALELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGSVVQLDRWNLQVERSVVQHEEGTQKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVRVVCDGTDLTAKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLAALQVGGHGDRLHQCREVLITTFKTLPVQVDGEPCRLAPSTLRISLRNQANMVQKSKRRTSVPLLNDIQKVCAADLRRLSAPPDSFSVPHAVPERLRLRVNRIGLQDYDSMQFDKERLRDISTAVGIVVVRGDCDLETCRLYIDRLQEDLHQAPSAGHRVHYQDECAGLPRPSSAHRLSANWSFLDSTSADRFYRIDKAQEHLHFVTEICQDEVYILDHEAPAVSQVRAPGMPDVVVEPSSGVPLTSDEQALLSAAAKGDLTAVSSSCRSGLSLLVRDAMGCTALHLAAHHGHADVVSFILEHGSKLMLDLTERDTGDTALHKAAAQQQRDVCKRLLEAGASPHKTNFLGKTPKDCALDAGNLELASMLEIQAVDEPASHEDLETAV

Target ORF information:

RefSeq Version	XM_009300039.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300039.2

ORF Insert Sequence:

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCATCT 
GCTCCCCGGA GGAAGTGTGC GGCCTGTAAG ATCGTGGTTC ACACCGCCTG CATCGAGGAG 
CTGGACAAGA TCAACTTCCG CTGTAAACCC ACTTTCAGAG AGGGCAGCTC TCGATGCCTC 
AGAGACCAGA ATGTGTTGCG GCACCACTGG GTTCATCGTC GCCGACAGGA GGGAAAATGT 
CGACAGTGCG GCAAAAGTTT TCCACAGAAG TTCTTCCACA GTAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAACA GGCGTTCCAC AACAAGGTGA CGTGTTTCAT GCTGCATCAG 
ATTGAGGAGC CCTGTTCACT GGGAGCTCAC GCCGGGGTCA TTGTCCCACC ATCCTGGATT 
ATTAAAGTCC GGAAACCGCA GAGCTCATTT AAAAACTCCA CTCGGAGGAA AAAACGGACG 
TCGTTCAAAA GAAGAGCCAG CAAAAAGGGC ACGGATGAAT CTAAATGGCG TCCATTCATG 
CTGAAGCCGC TGCCGTCACC GCTGATGAAA CCAGTGCTGG TGTTCGTCAA CCCGAAAAGT 
GGAGGAAACC AGGGCACGAA GCTCCTGCAG ATGTTCATGT GGATCCTGAA CCCTCGGCAG 
GTGTTTGATT TGTCTCAGGG AGGCCCCAGA GATGCGTTGG AGTTGTACAG GAAAGTGCCA 
AATCTTCGCA TCCTGGCCTG TGGAGGAGAT GGAACCGTGG GCTGGATTCT GTCGGCTTTA 
GATGAGCTTC AGATGAACCC TCAGCCTCCT GTGGCGGTAC TTCCTCTTGG AACAGGAAAT 
GACCTTGCTA GGACACTCAA CTGGGGAGGT GGCTACACAG ACGAGCCGGT GTCGAAGGTG 
TTGTGTCATG TGGAGGACGG CTCTGTGGTC CAGCTGGACC GCTGGAATCT GCAGGTGGAG 
CGCTCAGTGG TCCAGCACGA GGAGGGAACA CAGAAGCTTC CTTTAAATGT GTTCAATAAC 
TACTTCAGCC TCGGTTTCGA TGCCCACGTC ACCCTGGAGT TTCACGAGTC CAGAGAAGCA 
AACCCAGAGA AATTCAACAG TCGCTTCCGT AACAAGATGT TCTATGCAGG GGCTGCGTTC 
TCAGACTTCC TCCAGAGGAG CTCCAGGGAT CTGTCTAAGC ATGTCCGAGT TGTGTGTGAC 
GGCACCGATC TCACAGCCAA GATCCAGGAG CTCAAGTTTC AGTGCATCGT CTTCTTGAAT 
ATTCCCAGAT ACTGTGCTGG AACAATGCCA TGGGGAAACA CAGGAGACCA CCGAGACTTT 
GAGCCACAGC GACATGATGA TGGCTGCATC GAGGTCATCG GCTTCACCAT GGCCTCTCTG 
GCGGCGCTGC AGGTTGGTGG TCATGGGGAT CGTCTCCATC AGTGTCGAGA GGTGCTCATC 
ACCACCTTCA AGACTCTGCC AGTGCAGGTA GATGGAGAGC CGTGCCGTCT GGCGCCCTCC 
ACCCTGCGCA TCTCTCTGAG GAACCAGGCC AACATGGTGC AGAAGAGCAA GAGACGCACA 
TCTGTACCGC TGCTCAATGA CATTCAGAAA GTGTGTGCAG CCGACCTGCG CCGCCTCTCT 
GCTCCCCCAG ACTCCTTCTC TGTCCCTCAC GCCGTTCCAG AGCGCCTGCG TCTGCGTGTC 
AACCGCATCG GTCTGCAGGA CTACGACAGC ATGCAGTTCG ATAAGGAACG TCTGCGCGAC 
ATCTCGACTG CTGTTGGCAT CGTGGTGGTG AGAGGAGACT GTGACCTCGA AACCTGTCGA 
CTCTACATTG ATAGACTGCA GGAGGATTTG CATCAGGCGC CCTCTGCTGG CCACCGAGTG 
CACTATCAGG ATGAGTGTGC AGGTTTGCCA CGGCCGAGTT CAGCACACAG ACTTTCAGCC 
AACTGGAGCT TCCTGGACTC TACATCAGCT GATCGCTTTT ATCGCATCGA CAAGGCTCAG 
GAGCATCTTC ACTTTGTAAC TGAAATATGC CAGGATGAGG TTTATATCCT GGACCATGAA 
GCCCCAGCGG TGAGCCAGGT CAGGGCTCCA GGAATGCCTG ATGTGGTGGT GGAGCCCAGT 
TCTGGTGTCC CTCTGACATC AGATGAGCAA GCTCTGCTGT CGGCGGCTGC GAAAGGGGAC 
TTGACTGCGG TGTCATCGTC CTGCAGGAGT GGGCTTAGTC TGCTGGTGCG GGACGCTATG 
GGCTGCACCG CTCTGCATTT GGCTGCACAT CACGGACACG CAGACGTGGT GAGCTTCATT 
CTGGAGCATG GGTCAAAGCT GATGTTGGAT TTAACAGAGC GAGACACAGG TGACACAGCA 
TTGCACAAAG CGGCGGCACA ACAACAGCGT GATGTTTGCA AACGTCTGCT TGAAGCCGGA 
GCTTCCCCCC ACAAAACCAA CTTCCTGGGG AAAACACCTA AGGATTGTGC CCTGGATGCC 
GGGAATTTAG AGTTGGCGTC CATGCTGGAG ATCCAGGCGG TGGATGAACC AGCATCACAC 
GAGGATCTGG AGACGGCAGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_009300041.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298316.1 Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa49503
Clone ID Related Accession (Same CDS sequence)	XM_009300041.2
Accession Version	XM_009300041.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	diacylglycerol kinase iota isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300041.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACA ATGTGTTGCG GCACCACTGG GTTCATCGTC GCCGACAGGA GGGAAAATGT 
CGACAGTGCG GCAAAAGTTT TCCACAGAAG TTCTTCCACA GTAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAACA GGCGTTCCAC AACAAGGTGA CGTGTTTCAT GCTGCATCAG 
ATTGAGGAGC CCTGTTCACT GGGAGCTCAC GCCGGGGTCA TTGTCCCACC ATCCTGGATT 
ATTAAAGTCC GGAAACCGCA GAGCTCATTT AAAAACTCCA CTCGGAGGAA AAAACGGACG 
TCGTTCAAAA GAAGAGCCAG CAAAAAGGGC ACGGATGAAT CTAAATGGCG TCCATTCATG 
CTGAAGCCGC TGCCGTCACC GCTGATGAAA CCAGTGCTGG TGTTCGTCAA CCCGAAAAGT 
GGAGGAAACC AGGGCACGAA GCTCCTGCAG ATGTTCATGT GGATCCTGAA CCCTCGGCAG 
GTGTTTGATT TGTCTCAGGG AGGCCCCAGA GATGCGTTGG AGTTGTACAG GAAAGTGCCA 
AATCTTCGCA TCCTGGCCTG TGGAGGAGAT GGAACCGTGG GCTGGATTCT GTCGGCTTTA 
GATGAGCTTC AGATGAACCC TCAGCCTCCT GTGGCGGTAC TTCCTCTTGG AACAGGAAAT 
GACCTTGCTA GGACACTCAA CTGGGGAGGT GGCTACACAG ACGAGCCGGT GTCGAAGGTG 
TTGTGTCATG TGGAGGACGG CTCTGTGGTC CAGCTGGACC GCTGGAATCT GCAGGTGGAG 
CGCTCAGTGG TCCAGCACGA GGAGGGAACA CAGAAGCTTC CTTTAAATGT GTTCAATAAC 
TACTTCAGCC TCGGTTTCGA TGCCCACGTC ACCCTGGAGT TTCACGAGTC CAGAGAAGCA 
AACCCAGAGA AATTCAACAG TCGCTTCCGT AACAAGATGT TCTATGCAGG GGCTGCGTTC 
TCAGACTTCC TCCAGAGGAG CTCCAGGGAT CTGTCTAAGC ATGTCCGAGT TGTGTGTGAC 
GGCACCGATC TCACAGCCAA GATCCAGGAG CTCAAGTTTC AGTGCATCGT CTTCTTGAAT 
ATTCCCAGAT ACTGTGCTGG AACAATGCCA TGGGGAAACA CAGGAGACCA CCGAGACTTT 
GAGCCACAGC GACATGATGA TGGCTGCATC GAGGTCATCG GCTTCACCAT GGCCTCTCTG 
GCGGCGCTGC AGGTTGGTGG TCATGGGGAT CGTCTCCATC AGTGTCGAGA GGTGCTCATC 
ACCACCTTCA AGACTCTGCC AGTGCAGGTA GATGGAGAGC CGTGCCGTCT GGCGCCCTCC 
ACCCTGCGCA TCTCTCTGAG GAACCAGGCC AACATGGTGC AGAAGAGCAA GAGACGCACA 
TCTGTACCGC TGCTCAATGA CCCTCACGCC GTTCCAGAGC GCCTGCGTCT GCGTGTCAAC 
CGCATCGGTC TGCAGGACTA CGACAGCATG CAGTTCGATA AGGAACGTCT GCGCGACATC 
TCGACTGCTG TTGGCATCGT GGTGGTGAGA GGAGACTGTG ACCTCGAAAC CTGTCGACTC 
TACATTGATA GACTGCAGGA GGATTTGCAT CAGGCGCCCT CTGCTGGCCA CCGAGTGCAC 
TATCAGGATG AGTGTGCAGG TTTGCCACGG CCGAGTTCAG CACACAGACT TTCAGCCAAC 
TGGAGCTTCC TGGACTCTAC ATCAGCTGAT CGCTTTTATC GCATCGACAA GGCTCAGGAG 
CATCTTCACT TTGTAACTGA AATATGCCAG GATGAGGTTT ATATCCTGGA CCATGAAGCC 
CCAGCGGTGA GCCAGGTCAG GGCTCCAGGA ATGCCTGATG TGGTGGTGGA GCCCAGTTCT 
GGTGTCCCTC TGACATCAGA TGAGCAAGCT CTGCTGTCGG CGGCTGCGAA AGGGGACTTG 
ACTGCGGTGT CATCGTCCTG CAGGAGTGGG CTTAGTCTGC TGGTGCGGGA CGCTATGGGC 
TGCACCGCTC TGCATTTGGC TGCACATCAC GGACACGCAG ACGTGGTGAG CTTCATTCTG 
GAGCATGGGT CAAAGCTGAT GTTGGATTTA ACAGAGCGAG ACACAGGTGA CACAGCATTG 
CACAAAGCGG CGGCACAACA ACAGCGTGAT GTTTGCAAAC GTCTGCTTGA AGCCGGAGCT 
TCCCCCCACA AAACCAACTT CCTGGGGAAA ACACCTAAGG ATTGTGCCCT GGATGCCGGG 
AATTTAGAGT TGGCGTCCAT GCTGGAGATC CAGGCGGTGG ATGAACCAGC ATCACACGAG 
GATCTGGAGA CGGCAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298316.1
CDS	553..3393
Translation	MMDPLADMRASRAERSGRGAAGGESAEDQTRGCGKNAAMGGSDSLSSFADMDDSLEDKLKGLAFRKQISYRKAISRSGLQHLGPAQNPFPALSNGPAKELRNTVDWSENAVNGDHLWLETNCSGELCYLGEETCVAKIAKSAPRRKCAACKIVVHTACIEELDKINFRCKPTFREGSSRCLRDNVLRHHWVHRRRQEGKCRQCGKSFPQKFFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAGVIVPPSWIIKVRKPQSSFKNSTRRKKRTSFKRRASKKGTDESKWRPFMLKPLPSPLMKPVLVFVNPKSGGNQGTKLLQMFMWILNPRQVFDLSQGGPRDALELYRKVPNLRILACGGDGTVGWILSALDELQMNPQPPVAVLPLGTGNDLARTLNWGGGYTDEPVSKVLCHVEDGSVVQLDRWNLQVERSVVQHEEGTQKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVRVVCDGTDLTAKIQELKFQCIVFLNIPRYCAGTMPWGNTGDHRDFEPQRHDDGCIEVIGFTMASLAALQVGGHGDRLHQCREVLITTFKTLPVQVDGEPCRLAPSTLRISLRNQANMVQKSKRRTSVPLLNDPHAVPERLRLRVNRIGLQDYDSMQFDKERLRDISTAVGIVVVRGDCDLETCRLYIDRLQEDLHQAPSAGHRVHYQDECAGLPRPSSAHRLSANWSFLDSTSADRFYRIDKAQEHLHFVTEICQDEVYILDHEAPAVSQVRAPGMPDVVVEPSSGVPLTSDEQALLSAAAKGDLTAVSSSCRSGLSLLVRDAMGCTALHLAAHHGHADVVSFILEHGSKLMLDLTERDTGDTALHKAAAQQQRDVCKRLLEAGASPHKTNFLGKTPKDCALDAGNLELASMLEIQAVDEPASHEDLETAV

Target ORF information:

RefSeq Version	XM_009300041.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio diacylglycerol kinase, iota (dgki), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300041.2

ORF Insert Sequence:

ATGATGGACC CCCTAGCCGA CATGCGAGCG TCTCGGGCGG AGCGCAGCGG CAGAGGAGCC 
GCCGGTGGGG AATCGGCGGA AGATCAGACG CGCGGCTGCG GGAAAAACGC GGCGATGGGC 
GGCAGCGACA GCTTGAGCAG CTTCGCCGAC ATGGATGACT CGCTGGAGGA TAAACTCAAA 
GGTCTGGCGT TCAGGAAGCA GATCTCCTAT AGGAAAGCCA TCTCGCGCTC GGGCCTCCAG 
CATTTGGGTC CGGCCCAGAA TCCCTTCCCT GCCTTGAGCA ACGGACCAGC CAAGGAGCTC 
CGCAACACCG TGGACTGGAG TGAAAACGCA GTGAACGGTG ATCATCTGTG GCTGGAGACC 
AACTGCTCCG GTGAACTCTG CTACCTGGGT GAAGAAACCT GTGTGGCCAA GATCGCAAAG 
TCTGCTCCCC GGAGGAAGTG TGCGGCCTGT AAGATCGTGG TTCACACCGC CTGCATCGAG 
GAGCTGGACA AGATCAACTT CCGCTGTAAA CCCACTTTCA GAGAGGGCAG CTCTCGATGC 
CTCAGAGACA ATGTGTTGCG GCACCACTGG GTTCATCGTC GCCGACAGGA GGGAAAATGT 
CGACAGTGCG GCAAAAGTTT TCCACAGAAG TTCTTCCACA GTAAGGAGAT CGTGGCCATC 
AGCTGCTCCT GGTGCAAACA GGCGTTCCAC AACAAGGTGA CGTGTTTCAT GCTGCATCAG 
ATTGAGGAGC CCTGTTCACT GGGAGCTCAC GCCGGGGTCA TTGTCCCACC ATCCTGGATT 
ATTAAAGTCC GGAAACCGCA GAGCTCATTT AAAAACTCCA CTCGGAGGAA AAAACGGACG 
TCGTTCAAAA GAAGAGCCAG CAAAAAGGGC ACGGATGAAT CTAAATGGCG TCCATTCATG 
CTGAAGCCGC TGCCGTCACC GCTGATGAAA CCAGTGCTGG TGTTCGTCAA CCCGAAAAGT 
GGAGGAAACC AGGGCACGAA GCTCCTGCAG ATGTTCATGT GGATCCTGAA CCCTCGGCAG 
GTGTTTGATT TGTCTCAGGG AGGCCCCAGA GATGCGTTGG AGTTGTACAG GAAAGTGCCA 
AATCTTCGCA TCCTGGCCTG TGGAGGAGAT GGAACCGTGG GCTGGATTCT GTCGGCTTTA 
GATGAGCTTC AGATGAACCC TCAGCCTCCT GTGGCGGTAC TTCCTCTTGG AACAGGAAAT 
GACCTTGCTA GGACACTCAA CTGGGGAGGT GGCTACACAG ACGAGCCGGT GTCGAAGGTG 
TTGTGTCATG TGGAGGACGG CTCTGTGGTC CAGCTGGACC GCTGGAATCT GCAGGTGGAG 
CGCTCAGTGG TCCAGCACGA GGAGGGAACA CAGAAGCTTC CTTTAAATGT GTTCAATAAC 
TACTTCAGCC TCGGTTTCGA TGCCCACGTC ACCCTGGAGT TTCACGAGTC CAGAGAAGCA 
AACCCAGAGA AATTCAACAG TCGCTTCCGT AACAAGATGT TCTATGCAGG GGCTGCGTTC 
TCAGACTTCC TCCAGAGGAG CTCCAGGGAT CTGTCTAAGC ATGTCCGAGT TGTGTGTGAC 
GGCACCGATC TCACAGCCAA GATCCAGGAG CTCAAGTTTC AGTGCATCGT CTTCTTGAAT 
ATTCCCAGAT ACTGTGCTGG AACAATGCCA TGGGGAAACA CAGGAGACCA CCGAGACTTT 
GAGCCACAGC GACATGATGA TGGCTGCATC GAGGTCATCG GCTTCACCAT GGCCTCTCTG 
GCGGCGCTGC AGGTTGGTGG TCATGGGGAT CGTCTCCATC AGTGTCGAGA GGTGCTCATC 
ACCACCTTCA AGACTCTGCC AGTGCAGGTA GATGGAGAGC CGTGCCGTCT GGCGCCCTCC 
ACCCTGCGCA TCTCTCTGAG GAACCAGGCC AACATGGTGC AGAAGAGCAA GAGACGCACA 
TCTGTACCGC TGCTCAATGA CCCTCACGCC GTTCCAGAGC GCCTGCGTCT GCGTGTCAAC 
CGCATCGGTC TGCAGGACTA CGACAGCATG CAGTTCGATA AGGAACGTCT GCGCGACATC 
TCGACTGCTG TTGGCATCGT GGTGGTGAGA GGAGACTGTG ACCTCGAAAC CTGTCGACTC 
TACATTGATA GACTGCAGGA GGATTTGCAT CAGGCGCCCT CTGCTGGCCA CCGAGTGCAC 
TATCAGGATG AGTGTGCAGG TTTGCCACGG CCGAGTTCAG CACACAGACT TTCAGCCAAC 
TGGAGCTTCC TGGACTCTAC ATCAGCTGAT CGCTTTTATC GCATCGACAA GGCTCAGGAG 
CATCTTCACT TTGTAACTGA AATATGCCAG GATGAGGTTT ATATCCTGGA CCATGAAGCC 
CCAGCGGTGA GCCAGGTCAG GGCTCCAGGA ATGCCTGATG TGGTGGTGGA GCCCAGTTCT 
GGTGTCCCTC TGACATCAGA TGAGCAAGCT CTGCTGTCGG CGGCTGCGAA AGGGGACTTG 
ACTGCGGTGT CATCGTCCTG CAGGAGTGGG CTTAGTCTGC TGGTGCGGGA CGCTATGGGC 
TGCACCGCTC TGCATTTGGC TGCACATCAC GGACACGCAG ACGTGGTGAG CTTCATTCTG 
GAGCATGGGT CAAAGCTGAT GTTGGATTTA ACAGAGCGAG ACACAGGTGA CACAGCATTG 
CACAAAGCGG CGGCACAACA ACAGCGTGAT GTTTGCAAAC GTCTGCTTGA AGCCGGAGCT 
TCCCCCCACA AAACCAACTT CCTGGGGAAA ACACCTAAGG ATTGTGCCCT GGATGCCGGG 
AATTTAGAGT TGGCGTCCAT GCTGGAGATC CAGGCGGTGG ATGAACCAGC ATCACACGAG 
GATCTGGAGA CGGCAGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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DGKI cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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