DGKI cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	530095
Full Name	diacylglycerol kinase iota
General protein information	Preferred Names diacylglycerol kinase iota Names diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010804593.2	XP_010802895.2	diacylglycerol kinase iota isoform X1
XM_015470794.1	XP_015326280.1	diacylglycerol kinase iota isoform X2
XM_024991169.1	XP_024846937.1	diacylglycerol kinase iota isoform X2
XM_015463131.1	XP_015318617.1	diacylglycerol kinase iota isoform X1
XM_010804593.3	XP_010802895.2	diacylglycerol kinase iota isoform X1
XM_015463132.1	XP_015318618.1	diacylglycerol kinase iota isoform X2

Pathways from BioSystems

Homologies

	DGKI	XP_001107030.1
Canis lupus familiaris (dog)	DGKI	XP_005629798.1
Bos taurus (cattle)	DGKI	XP_002687107.1
Rattus norvegicus (Norway rat)	Dgki	NP_942077.2
Danio rerio (zebrafish)	dgki	XP_002667022.3
Xenopus tropicalis (tropical clawed frog)	dgki	XP_002942047.2
Gallus gallus (chicken)	DGKI	XP_416357.4
Homo sapiens (human)	DGKI	NP_004708.1
Pan troglodytes (chimpanzee)	DGKI	XP_001147695.1
Mus musculus (house mouse)	Dgki	NP_001074675.1

Gene Ontology

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa234050	XM_024991169.1 Latest version!	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa89784	XM_015463131.1 Latest version!	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa89784	XM_010804593.3 Latest version!	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa89785	XM_015463132.1 Latest version!	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa89785	XM_015470794.1 Latest version!	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa89784	XM_010804593.2	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_024991169.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846937.1 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa234050
Clone ID Related Accession (Same CDS sequence)	XM_024991169.1
Accession Version	XM_024991169.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACC GCTGGAAAGC AAAACACCCC TGGATGGGGG GAAGGAAAGC AATCTCTCGG 
GCAGGCCTGC AGCACTTGGC TCCTGCGCAT CCCCTCAGCC TACCTGTTGC AAACGGTCCA 
TCGAAGGAGC CCAGAGCGAC TCTGGACTGG AGCGAAAATG CCGTGAACGG AGAGCACCTG 
TGGCTGGAGA CCAACGTCTC GGGAGACCTG TGCTACCTGG GAGAGGAGAG CTGCCAAGTC 
AGATTTGCAA AATCAGCTCT CAGGAGGAAG TGTGCAGTCT GTAAGATTGT CGTCCATACC 
GCCTGCATTG AACAGCTAGA AAAGATTAAT TTCAGATGTA AACCAACATT TCGAGAAGGA 
GGTTCAAGAT CACCAAGAGA AAACTTTGTG CGTCACCACT GGGTGCATAG GCGTCGGCAG 
GAAGGAAAGT GTAAGCAGTG CGGTAAGGGC TTTCAGCAAA AATTCTCCTT CCACAGTAAA 
GAGATTGTGG CTATCAGCTG TTCCTGGTGC AAACAGGCGT TTCACAATAA GGTGACCTGC 
TTCATGCTGC ATCAGATTGA GGAACCCTGC TCCCTGGGGG CTCACGCTGC TGTTATTGTT 
CCGCCCACCT GGATCATTAA GGTGAAGAAA CCTCAGAACT CCCTGAAGGC ATCCAATCGG 
AAAAAGAAGA GAACAAGCTT TAAAAGGAAA GCCAGCAAAA GAGGAACCGA ACAGGAAAAC 
AAAGGTCGTC CTTTTGTGAT AAAACCTATC TCTTCTCCGC TCATGAAACC TTTGCTTGTG 
TTCGTGAACC CCAAGAGTGG AGGCAACCAG GGAACCAAAG TCCTACAGAT GTTCATGTGG 
TACCTGAACC CACGGCAGGT CTTTGACCTT TCTCAGGAAG GACCGAAAGA TGCGCTTGAG 
TTGTATAGGA AGGTACCAAA CCTGCGGATT CTGGCCTGTG GCGGGGATGG AACGGTGGGC 
TGGATCCTTT CCATTCTGGA TGAACTGCAG CTGAGCCCTC AGCCTCCTGT CGGGGTCCTT 
CCTCTGGGGA CTGGGAATGA CCTGGCTCGG ACTCTCAACT GGGGAGGGGG CTACACTGAT 
GAGCCTGTTT CTAAGATCCT TTGCCAAGTA GAAGATGGGA CGATTGTACA GCTGGATCGC 
TGGAATCTCC ACGTGGAAAG AAACCCAGAC TTGCCTCCTG AAGAACTGGA AGATGGCGTG 
TGTAAGCTTC CTCTGAATGT TTTCAATAAC TACTTCAGCC TCGGATTCGA TGCGCACGTC 
ACATTGGAGT TCCACGAATC CAGAGAAGCA AATCCAGAGA AATTCAACAG TCGTTTTCGA 
AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGATTTCC TACAGAGAAG CTCTAGAGAT 
TTATCCAAAC ATGTTAAAGT TGTCTGTGAT GGGACCGATC TTACCCCAAA GATCCAGGAA 
CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCC 
TGGGGGAACC CAGGAGATCA CCATGACTTT GAACCTCAGC GTCATGATGA TGGTTATATT 
GAAGTCATTG GGTTCACCAT GGCATCTTTG GCTGCCCTGC AAGTCGGAGG CCATGGAGAA 
AGGCTGCACC AGTGTCGGGA GGTCATGCTG CTAACTTACA AATCCATCCC CATGCAAGTG 
GATGGGGAGC CCTGCAGGTT GGCCCCGGCT ATGATTCGGA TCTCACTGAG GAATCAGGCC 
AACATGGTGC AGAAGAGCAA GAGGAGAACG TCCATGCCTT TACTCAATGA TCCCCAGTCT 
GTTCCAGACC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTACGACA AAGAGAAACT CCGGGAAGCC TCCATCCCAC TGGGCATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTGCAGGA GGACCTGCAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ACGAAACCTC CTTCCCTAGG 
GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCTAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTAATGGA GATTTCTCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCGGG AACACCGCCA 
GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCGGGAATGA CGGACTGGTG GAATCCTGCT 
CTCCGGAAAC GCATGCTGAG CGACAGTGGG CTGGGGATGA TAAGCCCGCA TTATGAAGAC 
TCGGATCTGA AAGATTTCAG CCACTCCCGA GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GCCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA TTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCGTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCAGCCTG CCAGCGGAAC 
CGGGCCGTGT GCCAGCTTCT GGTGGACGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGACAG AGCCCAACAG GCCGGGGACC CAGACTTGGC TGCCTACCTG 
GAAAGCCGCC AGAATTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846937.1
CDS	890..3706
Translation	MWDRWKAKHPWMGGRKAISRAGLQHLAPAHPLSLPVANGPSKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEESCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMISPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_024991169.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991169.1

ORF Insert Sequence:

ATGTGGGACC GCTGGAAAGC AAAACACCCC TGGATGGGGG GAAGGAAAGC AATCTCTCGG 
GCAGGCCTGC AGCACTTGGC TCCTGCGCAT CCCCTCAGCC TACCTGTTGC AAACGGTCCA 
TCGAAGGAGC CCAGAGCGAC TCTGGACTGG AGCGAAAATG CCGTGAACGG AGAGCACCTG 
TGGCTGGAGA CCAACGTCTC GGGAGACCTG TGCTACCTGG GAGAGGAGAG CTGCCAAGTC 
AGATTTGCAA AATCAGCTCT CAGGAGGAAG TGTGCAGTCT GTAAGATTGT CGTCCATACC 
GCCTGCATTG AACAGCTAGA AAAGATTAAT TTCAGATGTA AACCAACATT TCGAGAAGGA 
GGTTCAAGAT CACCAAGAGA AAACTTTGTG CGTCACCACT GGGTGCATAG GCGTCGGCAG 
GAAGGAAAGT GTAAGCAGTG CGGTAAGGGC TTTCAGCAAA AATTCTCCTT CCACAGTAAA 
GAGATTGTGG CTATCAGCTG TTCCTGGTGC AAACAGGCGT TTCACAATAA GGTGACCTGC 
TTCATGCTGC ATCAGATTGA GGAACCCTGC TCCCTGGGGG CTCACGCTGC TGTTATTGTT 
CCGCCCACCT GGATCATTAA GGTGAAGAAA CCTCAGAACT CCCTGAAGGC ATCCAATCGG 
AAAAAGAAGA GAACAAGCTT TAAAAGGAAA GCCAGCAAAA GAGGAACCGA ACAGGAAAAC 
AAAGGTCGTC CTTTTGTGAT AAAACCTATC TCTTCTCCGC TCATGAAACC TTTGCTTGTG 
TTCGTGAACC CCAAGAGTGG AGGCAACCAG GGAACCAAAG TCCTACAGAT GTTCATGTGG 
TACCTGAACC CACGGCAGGT CTTTGACCTT TCTCAGGAAG GACCGAAAGA TGCGCTTGAG 
TTGTATAGGA AGGTACCAAA CCTGCGGATT CTGGCCTGTG GCGGGGATGG AACGGTGGGC 
TGGATCCTTT CCATTCTGGA TGAACTGCAG CTGAGCCCTC AGCCTCCTGT CGGGGTCCTT 
CCTCTGGGGA CTGGGAATGA CCTGGCTCGG ACTCTCAACT GGGGAGGGGG CTACACTGAT 
GAGCCTGTTT CTAAGATCCT TTGCCAAGTA GAAGATGGGA CGATTGTACA GCTGGATCGC 
TGGAATCTCC ACGTGGAAAG AAACCCAGAC TTGCCTCCTG AAGAACTGGA AGATGGCGTG 
TGTAAGCTTC CTCTGAATGT TTTCAATAAC TACTTCAGCC TCGGATTCGA TGCGCACGTC 
ACATTGGAGT TCCACGAATC CAGAGAAGCA AATCCAGAGA AATTCAACAG TCGTTTTCGA 
AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGATTTCC TACAGAGAAG CTCTAGAGAT 
TTATCCAAAC ATGTTAAAGT TGTCTGTGAT GGGACCGATC TTACCCCAAA GATCCAGGAA 
CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCC 
TGGGGGAACC CAGGAGATCA CCATGACTTT GAACCTCAGC GTCATGATGA TGGTTATATT 
GAAGTCATTG GGTTCACCAT GGCATCTTTG GCTGCCCTGC AAGTCGGAGG CCATGGAGAA 
AGGCTGCACC AGTGTCGGGA GGTCATGCTG CTAACTTACA AATCCATCCC CATGCAAGTG 
GATGGGGAGC CCTGCAGGTT GGCCCCGGCT ATGATTCGGA TCTCACTGAG GAATCAGGCC 
AACATGGTGC AGAAGAGCAA GAGGAGAACG TCCATGCCTT TACTCAATGA TCCCCAGTCT 
GTTCCAGACC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTACGACA AAGAGAAACT CCGGGAAGCC TCCATCCCAC TGGGCATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTGCAGGA GGACCTGCAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ACGAAACCTC CTTCCCTAGG 
GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCTAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTAATGGA GATTTCTCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCGGG AACACCGCCA 
GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCGGGAATGA CGGACTGGTG GAATCCTGCT 
CTCCGGAAAC GCATGCTGAG CGACAGTGGG CTGGGGATGA TAAGCCCGCA TTATGAAGAC 
TCGGATCTGA AAGATTTCAG CCACTCCCGA GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GCCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA TTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCGTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCAGCCTG CCAGCGGAAC 
CGGGCCGTGT GCCAGCTTCT GGTGGACGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGACAG AGCCCAACAG GCCGGGGACC CAGACTTGGC TGCCTACCTG 
GAAAGCCGCC AGAATTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_010804593.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802895.2 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89784
Clone ID Related Accession (Same CDS sequence)	XM_010804593.2 , XM_015463131.1 , XM_010804593.3
Accession Version	XM_010804593.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG 
GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA 
CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG 
ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA 
GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA 
CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG 
GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC 
GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA 
AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA 
AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA 
GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG 
AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT 
GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC 
TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT 
CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG 
GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC 
TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT 
ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG 
GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG 
GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG 
TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG 
CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC 
TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA 
GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT 
CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG 
TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC 
TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG 
ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG 
GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802895.2
CDS	89..3097
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPGAAGPKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPSKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEESCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMISPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_010804593.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804593.2

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG 
GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA 
CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG 
ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA 
GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA 
CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG 
GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC 
GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA 
AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA 
AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA 
GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG 
AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT 
GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC 
TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT 
CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG 
GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC 
TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT 
ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG 
GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG 
GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG 
TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG 
CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC 
TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA 
GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT 
CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG 
TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC 
TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG 
ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG 
GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_015463131.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318617.1 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89784
Clone ID Related Accession (Same CDS sequence)	XM_010804593.2 , XM_015463131.1 , XM_010804593.3
Accession Version	XM_015463131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG 
GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA 
CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG 
ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA 
GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA 
CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG 
GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC 
GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA 
AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA 
AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA 
GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG 
AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT 
GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC 
TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT 
CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG 
GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC 
TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT 
ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG 
GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG 
GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG 
TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG 
CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC 
TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA 
GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT 
CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG 
TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC 
TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG 
ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG 
GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318617.1
CDS	89..3097
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPGAAGPKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPSKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEESCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMISPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_015463131.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463131.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG 
GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA 
CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG 
ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA 
GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA 
CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG 
GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC 
GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA 
AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA 
AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA 
GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG 
AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT 
GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC 
TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT 
CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG 
GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC 
TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT 
ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG 
GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG 
GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG 
TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG 
CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC 
TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA 
GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT 
CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG 
TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC 
TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG 
ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG 
GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_010804593.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802895.2 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89784
Clone ID Related Accession (Same CDS sequence)	XM_010804593.2 , XM_015463131.1 , XM_010804593.3
Accession Version	XM_010804593.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG 
GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA 
CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG 
ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA 
GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA 
CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG 
GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC 
GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA 
AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA 
AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA 
GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG 
AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT 
GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC 
TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT 
CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG 
GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC 
TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT 
ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG 
GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG 
GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG 
TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG 
CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC 
TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA 
GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT 
CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG 
TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC 
TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG 
ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG 
GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802895.2
CDS	84..3092
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPGAAGPKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPSKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEESCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMISPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_010804593.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010804593.3

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG 
GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA 
CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG 
ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA 
GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA 
CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG 
GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC 
GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA 
AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA 
AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA 
GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG 
AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT 
GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC 
TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT 
CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG 
GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC 
TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT 
ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG 
GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG 
GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG 
TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG 
CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC 
TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA 
GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT 
CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG 
TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC 
TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG 
ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG 
GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT 
GCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_015470794.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326280.1 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa89785
Clone ID Related Accession (Same CDS sequence)	XM_015470794.1 , XM_015463132.1
Accession Version	XM_015470794.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA 
AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC 
CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG 
TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG 
CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG 
AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT 
AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA 
TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA 
TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG 
CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC 
ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT 
CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG 
CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA 
ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC 
CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG 
GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG 
GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA 
AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326280.1
CDS	89..3082
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPGAAGPKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPSKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEESCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAAVIVPPTWIIKNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMISPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_015470794.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470794.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA 
AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC 
CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG 
TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG 
CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG 
AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT 
AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA 
TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA 
TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG 
CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC 
ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT 
CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG 
CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA 
ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC 
CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG 
GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG 
GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA 
AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_015463132.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318618.1 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa89785
Clone ID Related Accession (Same CDS sequence)	XM_015470794.1 , XM_015463132.1
Accession Version	XM_015463132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA 
AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC 
CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG 
TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG 
CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG 
AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT 
AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA 
TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA 
TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG 
CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC 
ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT 
CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG 
CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA 
ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC 
CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG 
GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG 
GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA 
AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318618.1
CDS	89..3082
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGAAAAAALEEPGAAGPKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPSKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEESCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHQIEEPCSLGAHAAVIVPPTWIIKNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGMTDWWNPALRKRMLSDSGLGMISPHYEDSDLKDFSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESRQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_015463132.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463132.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG 
GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC 
GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA 
GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT 
GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC 
GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG 
AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT 
GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA 
TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT 
AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC 
TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT 
AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT 
GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA 
AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC 
CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG 
TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG 
CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG 
AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT 
AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA 
TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA 
TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG 
CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC 
ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT 
CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG 
CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA 
ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC 
CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG 
GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG 
GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA 
AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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japanmarket@genscript.com

電話

03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

DGKI cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報