DGKI cDNA ORF clone, Bos taurus(cattle)

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234050 XM_024991169.1
Latest version!
Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa89784 XM_015463131.1
Latest version!
Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89784 XM_010804593.3
Latest version!
Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa89785 XM_015463132.1
Latest version!
Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OBa89785 XM_015470794.1
Latest version!
Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OBa89784 XM_010804593.2 Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234050
    Clone ID Related Accession (Same CDS sequence) XM_024991169.1
    Accession Version XM_024991169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGGACC GCTGGAAAGC AAAACACCCC TGGATGGGGG GAAGGAAAGC AATCTCTCGG 
    GCAGGCCTGC AGCACTTGGC TCCTGCGCAT CCCCTCAGCC TACCTGTTGC AAACGGTCCA
    TCGAAGGAGC CCAGAGCGAC TCTGGACTGG AGCGAAAATG CCGTGAACGG AGAGCACCTG
    TGGCTGGAGA CCAACGTCTC GGGAGACCTG TGCTACCTGG GAGAGGAGAG CTGCCAAGTC
    AGATTTGCAA AATCAGCTCT CAGGAGGAAG TGTGCAGTCT GTAAGATTGT CGTCCATACC
    GCCTGCATTG AACAGCTAGA AAAGATTAAT TTCAGATGTA AACCAACATT TCGAGAAGGA
    GGTTCAAGAT CACCAAGAGA AAACTTTGTG CGTCACCACT GGGTGCATAG GCGTCGGCAG
    GAAGGAAAGT GTAAGCAGTG CGGTAAGGGC TTTCAGCAAA AATTCTCCTT CCACAGTAAA
    GAGATTGTGG CTATCAGCTG TTCCTGGTGC AAACAGGCGT TTCACAATAA GGTGACCTGC
    TTCATGCTGC ATCAGATTGA GGAACCCTGC TCCCTGGGGG CTCACGCTGC TGTTATTGTT
    CCGCCCACCT GGATCATTAA GGTGAAGAAA CCTCAGAACT CCCTGAAGGC ATCCAATCGG
    AAAAAGAAGA GAACAAGCTT TAAAAGGAAA GCCAGCAAAA GAGGAACCGA ACAGGAAAAC
    AAAGGTCGTC CTTTTGTGAT AAAACCTATC TCTTCTCCGC TCATGAAACC TTTGCTTGTG
    TTCGTGAACC CCAAGAGTGG AGGCAACCAG GGAACCAAAG TCCTACAGAT GTTCATGTGG
    TACCTGAACC CACGGCAGGT CTTTGACCTT TCTCAGGAAG GACCGAAAGA TGCGCTTGAG
    TTGTATAGGA AGGTACCAAA CCTGCGGATT CTGGCCTGTG GCGGGGATGG AACGGTGGGC
    TGGATCCTTT CCATTCTGGA TGAACTGCAG CTGAGCCCTC AGCCTCCTGT CGGGGTCCTT
    CCTCTGGGGA CTGGGAATGA CCTGGCTCGG ACTCTCAACT GGGGAGGGGG CTACACTGAT
    GAGCCTGTTT CTAAGATCCT TTGCCAAGTA GAAGATGGGA CGATTGTACA GCTGGATCGC
    TGGAATCTCC ACGTGGAAAG AAACCCAGAC TTGCCTCCTG AAGAACTGGA AGATGGCGTG
    TGTAAGCTTC CTCTGAATGT TTTCAATAAC TACTTCAGCC TCGGATTCGA TGCGCACGTC
    ACATTGGAGT TCCACGAATC CAGAGAAGCA AATCCAGAGA AATTCAACAG TCGTTTTCGA
    AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGATTTCC TACAGAGAAG CTCTAGAGAT
    TTATCCAAAC ATGTTAAAGT TGTCTGTGAT GGGACCGATC TTACCCCAAA GATCCAGGAA
    CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCC
    TGGGGGAACC CAGGAGATCA CCATGACTTT GAACCTCAGC GTCATGATGA TGGTTATATT
    GAAGTCATTG GGTTCACCAT GGCATCTTTG GCTGCCCTGC AAGTCGGAGG CCATGGAGAA
    AGGCTGCACC AGTGTCGGGA GGTCATGCTG CTAACTTACA AATCCATCCC CATGCAAGTG
    GATGGGGAGC CCTGCAGGTT GGCCCCGGCT ATGATTCGGA TCTCACTGAG GAATCAGGCC
    AACATGGTGC AGAAGAGCAA GAGGAGAACG TCCATGCCTT TACTCAATGA TCCCCAGTCT
    GTTCCAGACC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC
    CACTACGACA AAGAGAAACT CCGGGAAGCC TCCATCCCAC TGGGCATTCT AGTTGTGCGT
    GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTGCAGGA GGACCTGCAG
    TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ACGAAACCTC CTTCCCTAGG
    GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCTAC TTCTGCTGAT
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTAATGGA GATTTCTCAA
    GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCGGG AACACCGCCA
    GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCGGGAATGA CGGACTGGTG GAATCCTGCT
    CTCCGGAAAC GCATGCTGAG CGACAGTGGG CTGGGGATGA TAAGCCCGCA TTATGAAGAC
    TCGGATCTGA AAGATTTCAG CCACTCCCGA GTGCTACAGT CACCAGTCTC TTCAGAAGAT
    CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA
    AATGGAGGCA GCCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA TTACGCAGCT
    AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCGTC CGAGTTATTG
    GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCAGCCTG CCAGCGGAAC
    CGGGCCGTGT GCCAGCTTCT GGTGGACGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG
    GGTAAGACAC CTCAAGACAG AGCCCAACAG GCCGGGGACC CAGACTTGGC TGCCTACCTG
    GAAAGCCGCC AGAATTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024846937.1
    CDS890..3706
    Translation

    Target ORF information:

    RefSeq Version XM_024991169.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGTGGGACC GCTGGAAAGC AAAACACCCC TGGATGGGGG GAAGGAAAGC AATCTCTCGG 
    GCAGGCCTGC AGCACTTGGC TCCTGCGCAT CCCCTCAGCC TACCTGTTGC AAACGGTCCA
    TCGAAGGAGC CCAGAGCGAC TCTGGACTGG AGCGAAAATG CCGTGAACGG AGAGCACCTG
    TGGCTGGAGA CCAACGTCTC GGGAGACCTG TGCTACCTGG GAGAGGAGAG CTGCCAAGTC
    AGATTTGCAA AATCAGCTCT CAGGAGGAAG TGTGCAGTCT GTAAGATTGT CGTCCATACC
    GCCTGCATTG AACAGCTAGA AAAGATTAAT TTCAGATGTA AACCAACATT TCGAGAAGGA
    GGTTCAAGAT CACCAAGAGA AAACTTTGTG CGTCACCACT GGGTGCATAG GCGTCGGCAG
    GAAGGAAAGT GTAAGCAGTG CGGTAAGGGC TTTCAGCAAA AATTCTCCTT CCACAGTAAA
    GAGATTGTGG CTATCAGCTG TTCCTGGTGC AAACAGGCGT TTCACAATAA GGTGACCTGC
    TTCATGCTGC ATCAGATTGA GGAACCCTGC TCCCTGGGGG CTCACGCTGC TGTTATTGTT
    CCGCCCACCT GGATCATTAA GGTGAAGAAA CCTCAGAACT CCCTGAAGGC ATCCAATCGG
    AAAAAGAAGA GAACAAGCTT TAAAAGGAAA GCCAGCAAAA GAGGAACCGA ACAGGAAAAC
    AAAGGTCGTC CTTTTGTGAT AAAACCTATC TCTTCTCCGC TCATGAAACC TTTGCTTGTG
    TTCGTGAACC CCAAGAGTGG AGGCAACCAG GGAACCAAAG TCCTACAGAT GTTCATGTGG
    TACCTGAACC CACGGCAGGT CTTTGACCTT TCTCAGGAAG GACCGAAAGA TGCGCTTGAG
    TTGTATAGGA AGGTACCAAA CCTGCGGATT CTGGCCTGTG GCGGGGATGG AACGGTGGGC
    TGGATCCTTT CCATTCTGGA TGAACTGCAG CTGAGCCCTC AGCCTCCTGT CGGGGTCCTT
    CCTCTGGGGA CTGGGAATGA CCTGGCTCGG ACTCTCAACT GGGGAGGGGG CTACACTGAT
    GAGCCTGTTT CTAAGATCCT TTGCCAAGTA GAAGATGGGA CGATTGTACA GCTGGATCGC
    TGGAATCTCC ACGTGGAAAG AAACCCAGAC TTGCCTCCTG AAGAACTGGA AGATGGCGTG
    TGTAAGCTTC CTCTGAATGT TTTCAATAAC TACTTCAGCC TCGGATTCGA TGCGCACGTC
    ACATTGGAGT TCCACGAATC CAGAGAAGCA AATCCAGAGA AATTCAACAG TCGTTTTCGA
    AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGATTTCC TACAGAGAAG CTCTAGAGAT
    TTATCCAAAC ATGTTAAAGT TGTCTGTGAT GGGACCGATC TTACCCCAAA GATCCAGGAA
    CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCC
    TGGGGGAACC CAGGAGATCA CCATGACTTT GAACCTCAGC GTCATGATGA TGGTTATATT
    GAAGTCATTG GGTTCACCAT GGCATCTTTG GCTGCCCTGC AAGTCGGAGG CCATGGAGAA
    AGGCTGCACC AGTGTCGGGA GGTCATGCTG CTAACTTACA AATCCATCCC CATGCAAGTG
    GATGGGGAGC CCTGCAGGTT GGCCCCGGCT ATGATTCGGA TCTCACTGAG GAATCAGGCC
    AACATGGTGC AGAAGAGCAA GAGGAGAACG TCCATGCCTT TACTCAATGA TCCCCAGTCT
    GTTCCAGACC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC
    CACTACGACA AAGAGAAACT CCGGGAAGCC TCCATCCCAC TGGGCATTCT AGTTGTGCGT
    GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTGCAGGA GGACCTGCAG
    TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ACGAAACCTC CTTCCCTAGG
    GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCTAC TTCTGCTGAT
    CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTAATGGA GATTTCTCAA
    GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCGGG AACACCGCCA
    GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCGGGAATGA CGGACTGGTG GAATCCTGCT
    CTCCGGAAAC GCATGCTGAG CGACAGTGGG CTGGGGATGA TAAGCCCGCA TTATGAAGAC
    TCGGATCTGA AAGATTTCAG CCACTCCCGA GTGCTACAGT CACCAGTCTC TTCAGAAGAT
    CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA
    AATGGAGGCA GCCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA TTACGCAGCT
    AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCGTC CGAGTTATTG
    GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCAGCCTG CCAGCGGAAC
    CGGGCCGTGT GCCAGCTTCT GGTGGACGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG
    GGTAAGACAC CTCAAGACAG AGCCCAACAG GCCGGGGACC CAGACTTGGC TGCCTACCTG
    GAAAGCCGCC AGAATTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89784
    Clone ID Related Accession (Same CDS sequence) XM_010804593.2 , XM_015463131.1 , XM_010804593.3
    Accession Version XM_010804593.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010804593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG
    GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC
    GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA
    CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG
    ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA
    GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT
    GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT
    GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG
    GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA
    CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG
    GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC
    GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC
    AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA
    AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA
    AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT
    GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT
    GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA
    GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC
    CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG
    AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT
    GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC
    TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT
    CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG
    GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC
    TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT
    ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG
    GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG
    GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG
    TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG
    CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC
    TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA
    GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT
    CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG
    TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC
    TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG
    ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG
    GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802895.2
    CDS89..3097
    Translation

    Target ORF information:

    RefSeq Version XM_010804593.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804593.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG
    GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC
    GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA
    CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG
    ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA
    GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT
    GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT
    GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG
    GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA
    CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG
    GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC
    GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC
    AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA
    AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA
    AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT
    GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT
    GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA
    GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC
    CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG
    AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT
    GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC
    TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT
    CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG
    GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC
    TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT
    ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG
    GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG
    GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG
    TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG
    CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC
    TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA
    GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT
    CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG
    TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC
    TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG
    ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG
    GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89784
    Clone ID Related Accession (Same CDS sequence) XM_010804593.2 , XM_015463131.1 , XM_010804593.3
    Accession Version XM_015463131.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG
    GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC
    GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA
    CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG
    ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA
    GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT
    GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT
    GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG
    GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA
    CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG
    GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC
    GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC
    AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA
    AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA
    AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT
    GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT
    GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA
    GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC
    CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG
    AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT
    GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC
    TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT
    CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG
    GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC
    TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT
    ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG
    GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG
    GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG
    TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG
    CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC
    TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA
    GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT
    CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG
    TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC
    TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG
    ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG
    GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318617.1
    CDS89..3097
    Translation

    Target ORF information:

    RefSeq Version XM_015463131.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463131.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG
    GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC
    GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA
    CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG
    ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA
    GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT
    GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT
    GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG
    GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA
    CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG
    GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC
    GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC
    AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA
    AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA
    AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT
    GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT
    GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA
    GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC
    CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG
    AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT
    GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC
    TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT
    CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG
    GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC
    TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT
    ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG
    GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG
    GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG
    TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG
    CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC
    TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA
    GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT
    CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG
    TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC
    TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG
    ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG
    GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89784
    Clone ID Related Accession (Same CDS sequence) XM_010804593.2 , XM_015463131.1 , XM_010804593.3
    Accession Version XM_010804593.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010804593.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG
    GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC
    GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA
    CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG
    ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA
    GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT
    GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT
    GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG
    GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA
    CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG
    GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC
    GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC
    AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA
    AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA
    AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT
    GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT
    GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA
    GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC
    CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG
    AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT
    GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC
    TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT
    CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG
    GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC
    TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT
    ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG
    GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG
    GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG
    TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG
    CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC
    TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA
    GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT
    CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG
    TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC
    TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG
    ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG
    GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010802895.2
    CDS84..3092
    Translation

    Target ORF information:

    RefSeq Version XM_010804593.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010804593.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGGTGAAGA AACCTCAGAA CTCCCTGAAG
    GCATCCAATC GGAAAAAGAA GAGAACAAGC TTTAAAAGGA AAGCCAGCAA AAGAGGAACC
    GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCTA TCTCTTCTCC GCTCATGAAA
    CCTTTGCTTG TGTTCGTGAA CCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTACAG
    ATGTTCATGT GGTACCTGAA CCCACGGCAG GTCTTTGACC TTTCTCAGGA AGGACCGAAA
    GATGCGCTTG AGTTGTATAG GAAGGTACCA AACCTGCGGA TTCTGGCCTG TGGCGGGGAT
    GGAACGGTGG GCTGGATCCT TTCCATTCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT
    GTCGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GGACTCTCAA CTGGGGAGGG
    GGCTACACTG ATGAGCCTGT TTCTAAGATC CTTTGCCAAG TAGAAGATGG GACGATTGTA
    CAGCTGGATC GCTGGAATCT CCACGTGGAA AGAAACCCAG ACTTGCCTCC TGAAGAACTG
    GAAGATGGCG TGTGTAAGCT TCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTCGGATTC
    GATGCGCACG TCACATTGGA GTTCCACGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC
    AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGATTT CCTACAGAGA
    AGCTCTAGAG ATTTATCCAA ACATGTTAAA GTTGTCTGTG ATGGGACCGA TCTTACCCCA
    AAGATCCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT
    GGCACAATGC CCTGGGGGAA CCCAGGAGAT CACCATGACT TTGAACCTCA GCGTCATGAT
    GATGGTTATA TTGAAGTCAT TGGGTTCACC ATGGCATCTT TGGCTGCCCT GCAAGTCGGA
    GGCCATGGAG AAAGGCTGCA CCAGTGTCGG GAGGTCATGC TGCTAACTTA CAAATCCATC
    CCCATGCAAG TGGATGGGGA GCCCTGCAGG TTGGCCCCGG CTATGATTCG GATCTCACTG
    AGGAATCAGG CCAACATGGT GCAGAAGAGC AAGAGGAGAA CGTCCATGCC TTTACTCAAT
    GATCCCCAGT CTGTTCCAGA CCGTCTGAGG ATCCGGGTGA ACAAAATCAG TTTACAAGAC
    TATGAAGGAT TCCACTACGA CAAAGAGAAA CTCCGGGAAG CCTCCATCCC ACTGGGCATT
    CTAGTTGTGC GTGGAGACTG TGATTTGGAG ACTTGCCGTA TGTACATAGA TCGCCTGCAG
    GAGGACCTGC AGTCAGTTTC TTCTGGCTCC CAGAGAGTTC ATTACCAGGA CCACGAAACC
    TCCTTCCCTA GGGCGCTCTC AGCTCAGAGA CTCTCCCCAC GGTGGTGCTT TCTAGATGCT
    ACTTCTGCTG ATCGCTTTTA TCGAATAGAC AGATCTCAGG AACATTTGCA CTTTGTAATG
    GAGATTTCTC AAGATGAGAT TTTTATTCTG GACCCAGATA TGGTGTTGTC ACAGCAGGCG
    GGAACACCGC CAGGAATGCC TGACCTGGTG GTGGAGCAAG CCTCGGGAAT GACGGACTGG
    TGGAATCCTG CTCTCCGGAA ACGCATGCTG AGCGACAGTG GGCTGGGGAT GATAAGCCCG
    CATTATGAAG ACTCGGATCT GAAAGATTTC AGCCACTCCC GAGTGCTACA GTCACCAGTC
    TCTTCAGAAG ATCACGCAAT TTTGCAGGCA GTAATAGCTG GTGATCTTAT GAAGCTAATA
    GAAAGCTATA AAAATGGAGG CAGCCTGCTA ATTCAGGGAC CAGACCACTG TTCACTCCTT
    CATTACGCAG CTAAAACCGG CAACGGGGAG ATTGTGAAAT ATATCCTTGA CCACGGACCG
    TCCGAGTTAT TGGATATGGC AGACAGTGAA ACGGGTGAGA CCGCACTGCA CAAGGCAGCC
    TGCCAGCGGA ACCGGGCCGT GTGCCAGCTT CTGGTGGACG CAGGAGCATC TCTGAGAAAG
    ACGGACTCCA AGGGTAAGAC ACCTCAAGAC AGAGCCCAAC AGGCCGGGGA CCCAGACTTG
    GCTGCCTACC TGGAAAGCCG CCAGAATTAT AAGATCATTG GCCATGAGGA CCTGGAAACT
    GCTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89785
    Clone ID Related Accession (Same CDS sequence) XM_015470794.1 , XM_015463132.1
    Accession Version XM_015470794.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103907.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA
    AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC
    CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG
    TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG
    CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG
    AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT
    AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA
    TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA
    TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG
    CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC
    ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT
    CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC
    TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA
    GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG
    CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT
    GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA
    ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC
    CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG
    GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC
    GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT
    GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA
    ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT
    ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG
    GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT
    AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA
    AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326280.1
    CDS89..3082
    Translation

    Target ORF information:

    RefSeq Version XM_015470794.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015470794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA
    AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC
    CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG
    TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG
    CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG
    AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT
    AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA
    TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA
    TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG
    CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC
    ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT
    CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC
    TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA
    GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG
    CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT
    GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA
    ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC
    CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG
    GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC
    GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT
    GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA
    ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT
    ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG
    GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT
    AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA
    AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89785
    Clone ID Related Accession (Same CDS sequence) XM_015470794.1 , XM_015463132.1
    Accession Version XM_015463132.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2994bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644739.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA
    AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC
    CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG
    TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG
    CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG
    AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT
    AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA
    TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA
    TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG
    CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC
    ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT
    CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC
    TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA
    GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG
    CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT
    GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA
    ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC
    CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG
    GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC
    GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT
    GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA
    ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT
    ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG
    GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT
    AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA
    AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015318618.1
    CDS89..3082
    Translation

    Target ORF information:

    RefSeq Version XM_015463132.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463132.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAGGGGGCGA CGGGCGGCAG CAGCAGCAGC 
    GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GAGGGGCGCG
    GGGGCAGCGG CGGCCGCTGC CCTGGAAGAG CCCGGGGCCG CCGGCCCGAA AGAGAAGGAC
    GAGGCACTGG AGGAAAAGCT GCGGAACTTA ACTTTCCGGA AGCAGGTCTC TTACAGGAAA
    GCAATCTCTC GGGCAGGCCT GCAGCACTTG GCTCCTGCGC ATCCCCTCAG CCTACCTGTT
    GCAAACGGTC CATCGAAGGA GCCCAGAGCG ACTCTGGACT GGAGCGAAAA TGCCGTGAAC
    GGAGAGCACC TGTGGCTGGA GACCAACGTC TCGGGAGACC TGTGCTACCT GGGAGAGGAG
    AGCTGCCAAG TCAGATTTGC AAAATCAGCT CTCAGGAGGA AGTGTGCAGT CTGTAAGATT
    GTCGTCCATA CCGCCTGCAT TGAACAGCTA GAAAAGATTA ATTTCAGATG TAAACCAACA
    TTTCGAGAAG GAGGTTCAAG ATCACCAAGA GAAAACTTTG TGCGTCACCA CTGGGTGCAT
    AGGCGTCGGC AGGAAGGAAA GTGTAAGCAG TGCGGTAAGG GCTTTCAGCA AAAATTCTCC
    TTCCACAGTA AAGAGATTGT GGCTATCAGC TGTTCCTGGT GCAAACAGGC GTTTCACAAT
    AAGGTGACCT GCTTCATGCT GCATCAGATT GAGGAACCCT GCTCCCTGGG GGCTCACGCT
    GCTGTTATTG TTCCGCCCAC CTGGATCATT AAGAACTCCC TGAAGGCATC CAATCGGAAA
    AAGAAGAGAA CAAGCTTTAA AAGGAAAGCC AGCAAAAGAG GAACCGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCGCTCA TGAAACCTTT GCTTGTGTTC
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TACAGATGTT CATGTGGTAC
    CTGAACCCAC GGCAGGTCTT TGACCTTTCT CAGGAAGGAC CGAAAGATGC GCTTGAGTTG
    TATAGGAAGG TACCAAACCT GCGGATTCTG GCCTGTGGCG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCCA TTCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGGACT CTCAACTGGG GAGGGGGCTA CACTGATGAG
    CCTGTTTCTA AGATCCTTTG CCAAGTAGAA GATGGGACGA TTGTACAGCT GGATCGCTGG
    AATCTCCACG TGGAAAGAAA CCCAGACTTG CCTCCTGAAG AACTGGAAGA TGGCGTGTGT
    AAGCTTCCTC TGAATGTTTT CAATAACTAC TTCAGCCTCG GATTCGATGC GCACGTCACA
    TTGGAGTTCC ACGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGCTC TAGAGATTTA
    TCCAAACATG TTAAAGTTGT CTGTGATGGG ACCGATCTTA CCCCAAAGAT CCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGACTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACCATGGC ATCTTTGGCT GCCCTGCAAG TCGGAGGCCA TGGAGAAAGG
    CTGCACCAGT GTCGGGAGGT CATGCTGCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GCAGGTTGGC CCCGGCTATG ATTCGGATCT CACTGAGGAA TCAGGCCAAC
    ATGGTGCAGA AGAGCAAGAG GAGAACGTCC ATGCCTTTAC TCAATGATCC CCAGTCTGTT
    CCAGACCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC
    TACGACAAAG AGAAACTCCG GGAAGCCTCC ATCCCACTGG GCATTCTAGT TGTGCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TGCAGGAGGA CCTGCAGTCA
    GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCACG AAACCTCCTT CCCTAGGGCG
    CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCTACTTC TGCTGATCGC
    TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TAATGGAGAT TTCTCAAGAT
    GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCGGGAAC ACCGCCAGGA
    ATGCCTGACC TGGTGGTGGA GCAAGCCTCG GGAATGACGG ACTGGTGGAA TCCTGCTCTC
    CGGAAACGCA TGCTGAGCGA CAGTGGGCTG GGGATGATAA GCCCGCATTA TGAAGACTCG
    GATCTGAAAG ATTTCAGCCA CTCCCGAGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC
    GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT
    GGAGGCAGCC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCATTA CGCAGCTAAA
    ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCGTCCGA GTTATTGGAT
    ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CAGCCTGCCA GCGGAACCGG
    GCCGTGTGCC AGCTTCTGGT GGACGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT
    AAGACACCTC AAGACAGAGC CCAACAGGCC GGGGACCCAG ACTTGGCTGC CTACCTGGAA
    AGCCGCCAGA ATTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.