DGKI cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	DGKI
Entrez Gene ID	482709
Full Name	diacylglycerol kinase iota
General protein information	Preferred Names diacylglycerol kinase iota Names diacylglycerol kinase iota
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014119885.1	XP_013975360.1	diacylglycerol kinase iota isoform X4
XM_022403760.1	XP_022259468.1	diacylglycerol kinase iota isoform X4
XM_014119884.1	XP_013975359.1	diacylglycerol kinase iota isoform X3
XM_022403757.1	XP_022259465.1	diacylglycerol kinase iota isoform X1
XM_022403759.1	XP_022259467.1	diacylglycerol kinase iota isoform X3
XM_014119882.1	XP_013975357.1	diacylglycerol kinase iota isoform X1
XM_014119883.1	XP_013975358.1	diacylglycerol kinase iota isoform X2
XM_022403758.1	XP_022259466.1	diacylglycerol kinase iota isoform X2

Pathways from BioSystems

Homologies

	DGKI	XP_001107030.1
Canis lupus familiaris (dog)	DGKI	XP_005629798.1
Bos taurus (cattle)	DGKI	XP_002687107.1
Rattus norvegicus (Norway rat)	Dgki	NP_942077.2
Danio rerio (zebrafish)	dgki	XP_002667022.3
Xenopus tropicalis (tropical clawed frog)	dgki	XP_002942047.2
Gallus gallus (chicken)	DGKI	XP_416357.4
Homo sapiens (human)	DGKI	NP_004708.1
Pan troglodytes (chimpanzee)	DGKI	XP_001147695.1
Mus musculus (house mouse)	Dgki	NP_001074675.1

Gene Ontology

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe293859	XM_022403757.1 Latest version!	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe293860	XM_022403758.1 Latest version!	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe293861	XM_022403759.1 Latest version!	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe293862	XM_022403760.1 Latest version!	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe40463	XM_014119884.1 Latest version!	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe40464	XM_014119885.1 Latest version!	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCe40461	XM_014119882.1 Latest version!	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OCe40462	XM_014119883.1 Latest version!	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DGKI ( XM_022403757.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259465.1 Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe293859
Clone ID Related Accession (Same CDS sequence)	XM_022403757.1
Accession Version	XM_022403757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2931bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC 
AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA 
TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA 
CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA 
CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT 
AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT 
ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT 
CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG 
ATCATTAAGG TCAAGAAGCC TCAGGTGATC TCCATACCAG GAGGTGAACT TGAACTTGGT 
TGGGCGGGAG ACGGACCTGG TTGGGCGGGA GACTGGAGCC CCTCAGAGAA TTTGGGATTC 
TTCCATTTTT ACTGGGAGGG TTGGCAGAAC TCATTGAAGG CTTCAAATCG GAAGAAGAAG 
AGAACAAGCT TTAAAAGAAA AGCCAGTAAA AGAGGAACTG AACAGGAAAA CAAAGGTCGT 
CCTTTTGTGA TAAAACCTAT CTCTTCTCCT CTCATGAAAC CCTTGCTTGT GTTTGTGAAC 
CCCAAGAGTG GAGGCAACCA GGGAACCAAA GTCCTGCAGA TGTTCATGTG GTACCTGAAT 
CCTCGGCAGG TCTTTGATCT TTCTCAGGAA GGGCCAAAAG ATGCGCTTGA GTTATATAGG 
AAAGTACCAA ATCTGCGAAT TCTGGCTTGT GGAGGGGATG GAACGGTGGG CTGGATCCTT 
TCTATTCTGG ATGAACTACA GCTGAGCCCT CAGCCTCCCG TCGGGGTCCT TCCTCTGGGG 
ACTGGGAATG ACCTGGCTCG AACTCTCAAC TGGGGAGGGG GCTACACTGA TGAACCTGTC 
TCTAAGATCC TTTGCCAAGT AGAAGATGGG ACAATCGTAC AGCTGGATCG TTGGAACCTC 
CATGTGGAAA GAAACCCAGA CTTGCCTCCA GAAGAACTTG AAGATGGCGT ATGTAAGCTC 
CCTCTGAATG TTTTCAATAA TTACTTCAGC CTTGGATTTG ATGCCCACGT CACACTGGAG 
TTCCATGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GTCGTTTTCG AAATAAAATG 
TTCTATGCAG GGGCAGCTTT TTCTGATTTC CTACAGAGAA GTTCTAGAGA TCTATCCAAA 
CATGTTAAAG TTGTTTGTGA TGGAACAGAT CTCACTCCAA AGATTCAGGA ACTGAAGTTC 
CAGTGTATAG TATTTTTAAA TATACCCAGA TATTGTGCTG GCACAATGCC CTGGGGGAAC 
CCAGGAGATC ACCATGATTT TGAACCTCAG CGTCATGATG ATGGTTATAT TGAAGTCATT 
GGGTTCACTA TGGCCTCTTT GGCTGCCCTG CAAGTTGGGG GCCATGGGGA AAGGCTACAC 
CAGTGTCGGG AGGTCATGCT GCTCACTTAC AAATCCATCC CCATGCAAGT GGATGGGGAG 
CCCTGTAGGT TGGCCCCGGC TATGATTCGG ATCTCCTTGA GGAATCAAGC CAACATGGTA 
CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTACTCAATG ATATGCACCA GGTGTCCGCT 
GGCGACCTGC GGAGAGTCTC GGCTCCCCCT GGCTCCTTCA CCACTCCCCA ATCTGTCCCA 
GACCGCCTGA GGATCCGGGT GAACAAAATC AGTTTACAAG ACTATGAAGG ATTCCACTAT 
GACAAAGAGA AACTTCGGGA AGCTTCTATC CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC 
TGTGATTTGG AGACTTGCCG TATGTACATA GATCGCCTAC AGGAGGACCT GCAGTCAGTT 
TCTTCTGGCT CCCAGAGAGT TCATTACCAG GACCATGAAA CCTCCTTCCC CAGGGCGCTC 
TCAGCTCAGA GACTCTCCCC ACGGTGGTGC TTTCTAGATG CAACTTCTGC TGATCGCTTT 
TACCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC CCAAGATGAG 
ATTTTTATTC TGGACCCAGA TATGGTGTTG TCACAGCAGG CAGGGACACC TCCAGGAATG 
CCTGACCTGG TGGTGGAGCA AGCCTCAGGG CTGTCAGAAT GGTGGAATCC TGCCCTACGG 
AAGCGTATCC TGAGCGACAG TGGTCTGGGA ACGATTACTC CGCATTATGA GGACTCCGAT 
TTGAGGGATC TCAGCCACTC ACGTGTGCTA CAGTCACCAG TCTCTTCAGA AGATCACGCA 
ATTTTGCAGG CAGTAATAGC TGGTGATCTT ATGAAGCTAA TGGAAAGCTA TAAAAATGGA 
GGCAGTCTGC TGATCCAGGG GCCGGACCAC TGCTCCCTCC TTCACCATGC AGCTAAAACT 
GGCCACGGGG AGATCGTAAA ATATATCCTG GACCACGGAC CTTCTGAGTT ATTGGATATG 
GCAGACAGTG AAACGGGTGA GACCGCACTG CACAAGGCTG CCTGCCAGCG GAACCGGGCT 
GTGTGCCAGC TTCTGGTGGA TGCGGGAGCA TCTCTGAGAA AGACGGACTC CAAGGGTAAG 
ACCCCTCAAG ACAGAGCACA GCAAGCTGGA GACCCAGATT TGGCTGCTTA CCTAGAAAGC 
TGCCAGAACT ATAAGATCAT TGGCCATGAG GACCTGGAAA CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259465.1
CDS	290..3220
Translation	MYGSRNQTTNIISPALIYIQSCDHNIQENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHAACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQVISIPGGELELGWAGDGPGWAGDWSPSENLGFFHFYWEGWQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDMHQVSAGDLRRVSAPPGSFTTPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_022403757.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403757.1

ORF Insert Sequence:

ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC 
AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA 
TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA 
CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA 
CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT 
AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT 
ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT 
CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG 
ATCATTAAGG TCAAGAAGCC TCAGGTGATC TCCATACCAG GAGGTGAACT TGAACTTGGT 
TGGGCGGGAG ACGGACCTGG TTGGGCGGGA GACTGGAGCC CCTCAGAGAA TTTGGGATTC 
TTCCATTTTT ACTGGGAGGG TTGGCAGAAC TCATTGAAGG CTTCAAATCG GAAGAAGAAG 
AGAACAAGCT TTAAAAGAAA AGCCAGTAAA AGAGGAACTG AACAGGAAAA CAAAGGTCGT 
CCTTTTGTGA TAAAACCTAT CTCTTCTCCT CTCATGAAAC CCTTGCTTGT GTTTGTGAAC 
CCCAAGAGTG GAGGCAACCA GGGAACCAAA GTCCTGCAGA TGTTCATGTG GTACCTGAAT 
CCTCGGCAGG TCTTTGATCT TTCTCAGGAA GGGCCAAAAG ATGCGCTTGA GTTATATAGG 
AAAGTACCAA ATCTGCGAAT TCTGGCTTGT GGAGGGGATG GAACGGTGGG CTGGATCCTT 
TCTATTCTGG ATGAACTACA GCTGAGCCCT CAGCCTCCCG TCGGGGTCCT TCCTCTGGGG 
ACTGGGAATG ACCTGGCTCG AACTCTCAAC TGGGGAGGGG GCTACACTGA TGAACCTGTC 
TCTAAGATCC TTTGCCAAGT AGAAGATGGG ACAATCGTAC AGCTGGATCG TTGGAACCTC 
CATGTGGAAA GAAACCCAGA CTTGCCTCCA GAAGAACTTG AAGATGGCGT ATGTAAGCTC 
CCTCTGAATG TTTTCAATAA TTACTTCAGC CTTGGATTTG ATGCCCACGT CACACTGGAG 
TTCCATGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GTCGTTTTCG AAATAAAATG 
TTCTATGCAG GGGCAGCTTT TTCTGATTTC CTACAGAGAA GTTCTAGAGA TCTATCCAAA 
CATGTTAAAG TTGTTTGTGA TGGAACAGAT CTCACTCCAA AGATTCAGGA ACTGAAGTTC 
CAGTGTATAG TATTTTTAAA TATACCCAGA TATTGTGCTG GCACAATGCC CTGGGGGAAC 
CCAGGAGATC ACCATGATTT TGAACCTCAG CGTCATGATG ATGGTTATAT TGAAGTCATT 
GGGTTCACTA TGGCCTCTTT GGCTGCCCTG CAAGTTGGGG GCCATGGGGA AAGGCTACAC 
CAGTGTCGGG AGGTCATGCT GCTCACTTAC AAATCCATCC CCATGCAAGT GGATGGGGAG 
CCCTGTAGGT TGGCCCCGGC TATGATTCGG ATCTCCTTGA GGAATCAAGC CAACATGGTA 
CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTACTCAATG ATATGCACCA GGTGTCCGCT 
GGCGACCTGC GGAGAGTCTC GGCTCCCCCT GGCTCCTTCA CCACTCCCCA ATCTGTCCCA 
GACCGCCTGA GGATCCGGGT GAACAAAATC AGTTTACAAG ACTATGAAGG ATTCCACTAT 
GACAAAGAGA AACTTCGGGA AGCTTCTATC CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC 
TGTGATTTGG AGACTTGCCG TATGTACATA GATCGCCTAC AGGAGGACCT GCAGTCAGTT 
TCTTCTGGCT CCCAGAGAGT TCATTACCAG GACCATGAAA CCTCCTTCCC CAGGGCGCTC 
TCAGCTCAGA GACTCTCCCC ACGGTGGTGC TTTCTAGATG CAACTTCTGC TGATCGCTTT 
TACCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC CCAAGATGAG 
ATTTTTATTC TGGACCCAGA TATGGTGTTG TCACAGCAGG CAGGGACACC TCCAGGAATG 
CCTGACCTGG TGGTGGAGCA AGCCTCAGGG CTGTCAGAAT GGTGGAATCC TGCCCTACGG 
AAGCGTATCC TGAGCGACAG TGGTCTGGGA ACGATTACTC CGCATTATGA GGACTCCGAT 
TTGAGGGATC TCAGCCACTC ACGTGTGCTA CAGTCACCAG TCTCTTCAGA AGATCACGCA 
ATTTTGCAGG CAGTAATAGC TGGTGATCTT ATGAAGCTAA TGGAAAGCTA TAAAAATGGA 
GGCAGTCTGC TGATCCAGGG GCCGGACCAC TGCTCCCTCC TTCACCATGC AGCTAAAACT 
GGCCACGGGG AGATCGTAAA ATATATCCTG GACCACGGAC CTTCTGAGTT ATTGGATATG 
GCAGACAGTG AAACGGGTGA GACCGCACTG CACAAGGCTG CCTGCCAGCG GAACCGGGCT 
GTGTGCCAGC TTCTGGTGGA TGCGGGAGCA TCTCTGAGAA AGACGGACTC CAAGGGTAAG 
ACCCCTCAAG ACAGAGCACA GCAAGCTGGA GACCCAGATT TGGCTGCTTA CCTAGAAAGC 
TGCCAGAACT ATAAGATCAT TGGCCATGAG GACCTGGAAA CTGCTGTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_022403758.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259466.1 Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe293860
Clone ID Related Accession (Same CDS sequence)	XM_022403758.1
Accession Version	XM_022403758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC 
AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA 
TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA 
CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA 
CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT 
AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT 
ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT 
CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG 
ATCATTAAGG TCAAGAAGCC TCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA 
ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT 
TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC 
AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT 
CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA 
GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT 
ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT 
GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT 
AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT 
GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT 
CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC 
CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC 
TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT 
GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG 
TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA 
GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG 
TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG 
TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC 
TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG 
AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATC CCCAATCTGT CCCAGACCGC 
CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA 
GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT 
TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT 
GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT 
CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA 
ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT 
ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC 
CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT 
ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG 
GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC 
GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT 
CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG 
AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259466.1
CDS	287..3031
Translation	MYGSRNQTTNIISPALIYIQSCDHNIQENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHAACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_022403758.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403758.1

ORF Insert Sequence:

ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC 
AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA 
TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA 
CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA 
CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT 
AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT 
ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT 
CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG 
ATCATTAAGG TCAAGAAGCC TCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA 
ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT 
TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC 
AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT 
CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA 
GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT 
ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT 
GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT 
AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT 
GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT 
CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC 
CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC 
TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT 
GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG 
TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA 
GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG 
TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG 
TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC 
TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG 
AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATC CCCAATCTGT CCCAGACCGC 
CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA 
GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT 
TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT 
GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT 
CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA 
ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT 
ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC 
CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT 
ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG 
GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC 
GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT 
CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG 
AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_022403759.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259467.1 Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe293861
Clone ID Related Accession (Same CDS sequence)	XM_022403759.1
Accession Version	XM_022403759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	diacylglycerol kinase iota isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG 
AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG 
CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT GCACCAGGTG 
TCCGCTGGCG ACCTGCGGAG AGTCTCGGCT CCCCCTGGCT CCTTCACCAC TCCCCAATCT 
GTCCCAGACC GCCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AAGAGAAACT TCGGGAAGCT TCTATCCCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTACAGGA GGACCTGCAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCAAC TTCTGCTGAT 
CGCTTTTACC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCAGG GACACCTCCA 
GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCAGGGCTGT CAGAATGGTG GAATCCTGCC 
CTACGGAAGC GTATCCTGAG CGACAGTGGT CTGGGAACGA TTACTCCGCA TTATGAGGAC 
TCCGATTTGA GGGATCTCAG CCACTCACGT GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATGGA AAGCTATAAA 
AATGGAGGCA GTCTGCTGAT CCAGGGGCCG GACCACTGCT CCCTCCTTCA CCATGCAGCT 
AAAACTGGCC ACGGGGAGAT CGTAAAATAT ATCCTGGACC ACGGACCTTC TGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCG GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACCC CTCAAGACAG AGCACAGCAA GCTGGAGACC CAGATTTGGC TGCTTACCTA 
GAAAGCTGCC AGAACTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259467.1
CDS	522..2858
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDMHQVSAGDLRRVSAPPGSFTTPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_022403759.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403759.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG 
AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG 
CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT GCACCAGGTG 
TCCGCTGGCG ACCTGCGGAG AGTCTCGGCT CCCCCTGGCT CCTTCACCAC TCCCCAATCT 
GTCCCAGACC GCCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AAGAGAAACT TCGGGAAGCT TCTATCCCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTACAGGA GGACCTGCAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCAAC TTCTGCTGAT 
CGCTTTTACC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCAGG GACACCTCCA 
GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCAGGGCTGT CAGAATGGTG GAATCCTGCC 
CTACGGAAGC GTATCCTGAG CGACAGTGGT CTGGGAACGA TTACTCCGCA TTATGAGGAC 
TCCGATTTGA GGGATCTCAG CCACTCACGT GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATGGA AAGCTATAAA 
AATGGAGGCA GTCTGCTGAT CCAGGGGCCG GACCACTGCT CCCTCCTTCA CCATGCAGCT 
AAAACTGGCC ACGGGGAGAT CGTAAAATAT ATCCTGGACC ACGGACCTTC TGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCG GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACCC CTCAAGACAG AGCACAGCAA GCTGGAGACC CAGATTTGGC TGCTTACCTA 
GAAAGCTGCC AGAACTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_022403760.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022259468.1 Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe293862
Clone ID Related Accession (Same CDS sequence)	XM_022403760.1
Accession Version	XM_022403760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	diacylglycerol kinase iota isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG 
AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG 
CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAATCTGTC 
CCAGACCGCC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAAG AGAAACTTCG GGAAGCTTCT ATCCCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TACAGGAGGA CCTGCAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCG 
CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCAACTTC TGCTGATCGC 
TTTTACCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCAGGGAC ACCTCCAGGA 
ATGCCTGACC TGGTGGTGGA GCAAGCCTCA GGGCTGTCAG AATGGTGGAA TCCTGCCCTA 
CGGAAGCGTA TCCTGAGCGA CAGTGGTCTG GGAACGATTA CTCCGCATTA TGAGGACTCC 
GATTTGAGGG ATCTCAGCCA CTCACGTGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATGGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTGATCCA GGGGCCGGAC CACTGCTCCC TCCTTCACCA TGCAGCTAAA 
ACTGGCCACG GGGAGATCGT AAAATATATC CTGGACCACG GACCTTCTGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCGGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACCCCTC AAGACAGAGC ACAGCAAGCT GGAGACCCAG ATTTGGCTGC TTACCTAGAA 
AGCTGCCAGA ACTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022259468.1
CDS	522..2795
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_022403760.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022403760.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT 
GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG 
AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG 
CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAATCTGTC 
CCAGACCGCC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAAG AGAAACTTCG GGAAGCTTCT ATCCCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TACAGGAGGA CCTGCAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCG 
CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCAACTTC TGCTGATCGC 
TTTTACCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCAGGGAC ACCTCCAGGA 
ATGCCTGACC TGGTGGTGGA GCAAGCCTCA GGGCTGTCAG AATGGTGGAA TCCTGCCCTA 
CGGAAGCGTA TCCTGAGCGA CAGTGGTCTG GGAACGATTA CTCCGCATTA TGAGGACTCC 
GATTTGAGGG ATCTCAGCCA CTCACGTGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATGGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTGATCCA GGGGCCGGAC CACTGCTCCC TCCTTCACCA TGCAGCTAAA 
ACTGGCCACG GGGAGATCGT AAAATATATC CTGGACCACG GACCTTCTGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCGGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACCCCTC AAGACAGAGC ACAGCAAGCT GGAGACCCAG ATTTGGCTGC TTACCTAGAA 
AGCTGCCAGA ACTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_014119884.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975359.1 Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe40463
Clone ID Related Accession (Same CDS sequence)	XM_014119884.1
Accession Version	XM_014119884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2925bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	diacylglycerol kinase iota isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA 
AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT 
GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG 
AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG 
CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA 
CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT 
CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG 
AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG 
ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG 
GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG 
AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT 
GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT 
GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT 
AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT 
ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA 
GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC 
ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT 
CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT 
AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG 
AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC 
CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC 
CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA 
GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT 
TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT 
GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT 
CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA 
ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT 
ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC 
CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT 
ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG 
GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC 
GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT 
CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG 
AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975359.1
CDS	182..3106
Translation	MSPGWVDLFCRSCRNQDRCTTPVPEASDGWKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHAACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDMHQVSAGDLRRVSAPPGSFTTPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_014119884.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119884.1

ORF Insert Sequence:

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA 
AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT 
GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG 
AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG 
CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA 
CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT 
CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG 
AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG 
ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG 
GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG 
AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT 
GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT 
GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT 
AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT 
ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA 
GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC 
ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT 
CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT 
AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG 
AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC 
CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC 
CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA 
GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT 
TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT 
GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT 
CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA 
ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT 
ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC 
CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT 
ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG 
GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC 
GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT 
CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG 
AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_014119885.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975360.1 Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe40464
Clone ID Related Accession (Same CDS sequence)	XM_014119885.1
Accession Version	XM_014119885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	diacylglycerol kinase iota isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA 
AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT 
GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG 
AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG 
CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA 
CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT 
CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG 
AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG 
ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG 
GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG 
AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT 
GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT 
GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT 
AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT 
ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA 
GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC 
ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT 
CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT 
AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG 
AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATCCCC AATCTGTCCC AGACCGCCTG 
AGGATCCGGG TGAACAAAAT CAGTTTACAA GACTATGAAG GATTCCACTA TGACAAAGAG 
AAACTTCGGG AAGCTTCTAT CCCTCTGGGT ATTCTAGTTG TGCGTGGAGA CTGTGATTTG 
GAGACTTGCC GTATGTACAT AGATCGCCTA CAGGAGGACC TGCAGTCAGT TTCTTCTGGC 
TCCCAGAGAG TTCATTACCA GGACCATGAA ACCTCCTTCC CCAGGGCGCT CTCAGCTCAG 
AGACTCTCCC CACGGTGGTG CTTTCTAGAT GCAACTTCTG CTGATCGCTT TTACCGAATA 
GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CCCAAGATGA GATTTTTATT 
CTGGACCCAG ATATGGTGTT GTCACAGCAG GCAGGGACAC CTCCAGGAAT GCCTGACCTG 
GTGGTGGAGC AAGCCTCAGG GCTGTCAGAA TGGTGGAATC CTGCCCTACG GAAGCGTATC 
CTGAGCGACA GTGGTCTGGG AACGATTACT CCGCATTATG AGGACTCCGA TTTGAGGGAT 
CTCAGCCACT CACGTGTGCT ACAGTCACCA GTCTCTTCAG AAGATCACGC AATTTTGCAG 
GCAGTAATAG CTGGTGATCT TATGAAGCTA ATGGAAAGCT ATAAAAATGG AGGCAGTCTG 
CTGATCCAGG GGCCGGACCA CTGCTCCCTC CTTCACCATG CAGCTAAAAC TGGCCACGGG 
GAGATCGTAA AATATATCCT GGACCACGGA CCTTCTGAGT TATTGGATAT GGCAGACAGT 
GAAACGGGTG AGACCGCACT GCACAAGGCT GCCTGCCAGC GGAACCGGGC TGTGTGCCAG 
CTTCTGGTGG ATGCGGGAGC ATCTCTGAGA AAGACGGACT CCAAGGGTAA GACCCCTCAA 
GACAGAGCAC AGCAAGCTGG AGACCCAGAT TTGGCTGCTT ACCTAGAAAG CTGCCAGAAC 
TATAAGATCA TTGGCCATGA GGACCTGGAA ACTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975360.1
CDS	179..3040
Translation	MSPGWVDLFCRSCRNQDRCTTPVPEASDGWKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHAACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_014119885.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119885.1

ORF Insert Sequence:

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA 
AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT 
GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG 
AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG 
CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA 
CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT 
CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG 
AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG 
ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG 
GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG 
AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT 
GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT 
GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT 
AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT 
ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA 
GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC 
ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT 
CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT 
AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG 
AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATCCCC AATCTGTCCC AGACCGCCTG 
AGGATCCGGG TGAACAAAAT CAGTTTACAA GACTATGAAG GATTCCACTA TGACAAAGAG 
AAACTTCGGG AAGCTTCTAT CCCTCTGGGT ATTCTAGTTG TGCGTGGAGA CTGTGATTTG 
GAGACTTGCC GTATGTACAT AGATCGCCTA CAGGAGGACC TGCAGTCAGT TTCTTCTGGC 
TCCCAGAGAG TTCATTACCA GGACCATGAA ACCTCCTTCC CCAGGGCGCT CTCAGCTCAG 
AGACTCTCCC CACGGTGGTG CTTTCTAGAT GCAACTTCTG CTGATCGCTT TTACCGAATA 
GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CCCAAGATGA GATTTTTATT 
CTGGACCCAG ATATGGTGTT GTCACAGCAG GCAGGGACAC CTCCAGGAAT GCCTGACCTG 
GTGGTGGAGC AAGCCTCAGG GCTGTCAGAA TGGTGGAATC CTGCCCTACG GAAGCGTATC 
CTGAGCGACA GTGGTCTGGG AACGATTACT CCGCATTATG AGGACTCCGA TTTGAGGGAT 
CTCAGCCACT CACGTGTGCT ACAGTCACCA GTCTCTTCAG AAGATCACGC AATTTTGCAG 
GCAGTAATAG CTGGTGATCT TATGAAGCTA ATGGAAAGCT ATAAAAATGG AGGCAGTCTG 
CTGATCCAGG GGCCGGACCA CTGCTCCCTC CTTCACCATG CAGCTAAAAC TGGCCACGGG 
GAGATCGTAA AATATATCCT GGACCACGGA CCTTCTGAGT TATTGGATAT GGCAGACAGT 
GAAACGGGTG AGACCGCACT GCACAAGGCT GCCTGCCAGC GGAACCGGGC TGTGTGCCAG 
CTTCTGGTGG ATGCGGGAGC ATCTCTGAGA AAGACGGACT CCAAGGGTAA GACCCCTCAA 
GACAGAGCAC AGCAAGCTGG AGACCCAGAT TTGGCTGCTT ACCTAGAAAG CTGCCAGAAC 
TATAAGATCA TTGGCCATGA GGACCTGGAA ACTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_014119882.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975357.1 Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe40461
Clone ID Related Accession (Same CDS sequence)	XM_014119882.1
Accession Version	XM_014119882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3048bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	diacylglycerol kinase iota isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG 
GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC 
CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA 
ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT 
TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC 
AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT 
CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA 
GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT 
ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT 
GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT 
AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT 
GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT 
CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC 
CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC 
TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT 
GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG 
TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA 
GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG 
TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG 
TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC 
TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG 
AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATA TGCACCAGGT GTCCGCTGGC 
GACCTGCGGA GAGTCTCGGC TCCCCCTGGC TCCTTCACCA CTCCCCAATC TGTCCCAGAC 
CGCCTGAGGA TCCGGGTGAA CAAAATCAGT TTACAAGACT ATGAAGGATT CCACTATGAC 
AAAGAGAAAC TTCGGGAAGC TTCTATCCCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAT CGCCTACAGG AGGACCTGCA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCGCTCTCA 
GCTCAGAGAC TCTCCCCACG GTGGTGCTTT CTAGATGCAA CTTCTGCTGA TCGCTTTTAC 
CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT 
TTTATTCTGG ACCCAGATAT GGTGTTGTCA CAGCAGGCAG GGACACCTCC AGGAATGCCT 
GACCTGGTGG TGGAGCAAGC CTCAGGGCTG TCAGAATGGT GGAATCCTGC CCTACGGAAG 
CGTATCCTGA GCGACAGTGG TCTGGGAACG ATTACTCCGC ATTATGAGGA CTCCGATTTG 
AGGGATCTCA GCCACTCACG TGTGCTACAG TCACCAGTCT CTTCAGAAGA TCACGCAATT 
TTGCAGGCAG TAATAGCTGG TGATCTTATG AAGCTAATGG AAAGCTATAA AAATGGAGGC 
AGTCTGCTGA TCCAGGGGCC GGACCACTGC TCCCTCCTTC ACCATGCAGC TAAAACTGGC 
CACGGGGAGA TCGTAAAATA TATCCTGGAC CACGGACCTT CTGAGTTATT GGATATGGCA 
GACAGTGAAA CGGGTGAGAC CGCACTGCAC AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG 
TGCCAGCTTC TGGTGGATGC GGGAGCATCT CTGAGAAAGA CGGACTCCAA GGGTAAGACC 
CCTCAAGACA GAGCACAGCA AGCTGGAGAC CCAGATTTGG CTGCTTACCT AGAAAGCTGC 
CAGAACTATA AGATCATTGG CCATGAGGAC CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975357.1
CDS	199..3246
Translation	MSPGWVDLFCRSCRNQDRCTTPVPEASDGWKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHAACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQVISIPGGELELGWAGDGPGWAGDWSPSENLGFFHFYWEGWQNSLKASNRKKKRTSFKRKASKRGTEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDMHQVSAGDLRRVSAPPGSFTTPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_014119882.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119882.1

ORF Insert Sequence:

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG 
GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC 
CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA 
ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT 
TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC 
AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT 
CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA 
GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT 
ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT 
GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT 
AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT 
GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT 
CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC 
CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC 
TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT 
GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG 
TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA 
GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG 
TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG 
TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC 
TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG 
AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATA TGCACCAGGT GTCCGCTGGC 
GACCTGCGGA GAGTCTCGGC TCCCCCTGGC TCCTTCACCA CTCCCCAATC TGTCCCAGAC 
CGCCTGAGGA TCCGGGTGAA CAAAATCAGT TTACAAGACT ATGAAGGATT CCACTATGAC 
AAAGAGAAAC TTCGGGAAGC TTCTATCCCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAT CGCCTACAGG AGGACCTGCA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCGCTCTCA 
GCTCAGAGAC TCTCCCCACG GTGGTGCTTT CTAGATGCAA CTTCTGCTGA TCGCTTTTAC 
CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT 
TTTATTCTGG ACCCAGATAT GGTGTTGTCA CAGCAGGCAG GGACACCTCC AGGAATGCCT 
GACCTGGTGG TGGAGCAAGC CTCAGGGCTG TCAGAATGGT GGAATCCTGC CCTACGGAAG 
CGTATCCTGA GCGACAGTGG TCTGGGAACG ATTACTCCGC ATTATGAGGA CTCCGATTTG 
AGGGATCTCA GCCACTCACG TGTGCTACAG TCACCAGTCT CTTCAGAAGA TCACGCAATT 
TTGCAGGCAG TAATAGCTGG TGATCTTATG AAGCTAATGG AAAGCTATAA AAATGGAGGC 
AGTCTGCTGA TCCAGGGGCC GGACCACTGC TCCCTCCTTC ACCATGCAGC TAAAACTGGC 
CACGGGGAGA TCGTAAAATA TATCCTGGAC CACGGACCTT CTGAGTTATT GGATATGGCA 
GACAGTGAAA CGGGTGAGAC CGCACTGCAC AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG 
TGCCAGCTTC TGGTGGATGC GGGAGCATCT CTGAGAAAGA CGGACTCCAA GGGTAAGACC 
CCTCAAGACA GAGCACAGCA AGCTGGAGAC CCAGATTTGG CTGCTTACCT AGAAAGCTGC 
CAGAACTATA AGATCATTGG CCATGAGGAC CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_014119883.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013975358.1 Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe40462
Clone ID Related Accession (Same CDS sequence)	XM_014119883.1
Accession Version	XM_014119883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	diacylglycerol kinase iota isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG 
GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC 
CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA 
ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAG AAAACAAAGG TCGTCCTTTT 
GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG 
AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG 
CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA 
CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT 
CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG 
AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG 
ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG 
GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG 
AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT 
GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT 
GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT 
AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT 
ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA 
GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC 
ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT 
CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT 
AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG 
AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC 
CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC 
CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA 
GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT 
TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT 
GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT 
CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA 
ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT 
ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC 
CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT 
ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG 
GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC 
GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT 
CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG 
AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975358.1
CDS	322..3366
Translation	MSPGWVDLFCRSCRNQDRCTTPVPEASDGWKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHAACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQVISIPGGELELGWAGDGPGWAGDWSPSENLGFFHFYWEGWQNSLKASNRKKKRTSFKRKASKRGTEENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTIVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDMHQVSAGDLRRVSAPPGSFTTPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVLSQQAGTPPGMPDLVVEQASGLSEWWNPALRKRILSDSGLGTITPHYEDSDLRDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLMESYKNGGSLLIQGPDHCSLLHHAAKTGHGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQDRAQQAGDPDLAAYLESCQNYKIIGHEDLETAV

Target ORF information:

RefSeq Version	XM_014119883.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119883.1

ORF Insert Sequence:

ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT 
CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA 
GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT 
GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC 
GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG 
CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA 
AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG 
CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC 
AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC 
ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC 
ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG 
GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC 
CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA 
ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAG AAAACAAAGG TCGTCCTTTT 
GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG 
AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG 
CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA 
CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT 
CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG 
AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG 
ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG 
GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG 
AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT 
GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT 
GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT 
AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT 
ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA 
GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC 
ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT 
CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT 
AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG 
AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC 
CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC 
CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA 
GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT 
TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT 
GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT 
CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA 
ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT 
ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC 
CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT 
ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG 
GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC 
GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT 
CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG 
AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

DGKI cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報