DGKI gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001321710.2	NP_001308639.1	diacylglycerol kinase iota isoform 4
XM_017012786.1	XP_016868275.1	diacylglycerol kinase iota isoform X8
XM_017012787.1	XP_016868276.1	diacylglycerol kinase iota isoform X8
XM_017012780.1	XP_016868269.1	diacylglycerol kinase iota isoform X1
NM_004717.2	NP_004708.1	diacylglycerol kinase iota
XM_011516684.2	XP_011514986.2	diacylglycerol kinase iota isoform X10
XM_017012781.1	XP_016868270.1	diacylglycerol kinase iota isoform X2
XM_017012783.1	XP_016868272.1	diacylglycerol kinase iota isoform X4
NM_001321709.1	NP_001308638.1	diacylglycerol kinase iota isoform 3
XM_017012788.2	XP_016868277.1	diacylglycerol kinase iota isoform X2
XM_017012780.2	XP_016868269.1	diacylglycerol kinase iota isoform X1
XM_017012789.1	XP_016868278.1	diacylglycerol kinase iota isoform X11
NM_001321710.1	NP_001308639.1	diacylglycerol kinase iota isoform 4
XM_017012782.1	XP_016868271.1	diacylglycerol kinase iota isoform X3
XM_017012784.1	XP_016868273.1	diacylglycerol kinase iota isoform X5
XM_011516682.2	XP_011514984.2	diacylglycerol kinase iota isoform X6
XM_017012785.1	XP_016868274.1	diacylglycerol kinase iota isoform X7
XM_017012788.1	XP_016868277.1	diacylglycerol kinase iota isoform X9
NM_004717.3	NP_004708.1	diacylglycerol kinase iota isoform 1
NM_001321708.1	NP_001308637.1	diacylglycerol kinase iota isoform 2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17163	NM_004717.3 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$539.50-$755.30 $1079.00
OHu101649	NM_001321710.2 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97596	NM_001321709.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97594	XM_017012785.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu97595	XM_017012786.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97595	XM_017012787.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97596	XM_017012788.2 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97597	XM_011516684.2 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97598	XM_017012789.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OHu101847	NM_001321708.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu97588	XM_017012780.2 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu97589	XM_017012781.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu97590	XM_017012782.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu97591	XM_017012783.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu97592	XM_017012784.1 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu97593	XM_011516682.2 Latest version!	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu97588	XM_017012780.1	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu101649	NM_001321710.1	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu97596	XM_017012788.1	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu17163	NM_004717.2	Homo sapiens diacylglycerol kinase, iota (DGKI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$539.50-$755.30 $1079.00
Hide Old Accession Versions >>

DGKI ( NM_004717.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004708.1 Homo sapiens diacylglycerol kinase, iota (DGKI), mRNA.

Price & Availability↑

CloneID	OHu17163
Clone ID Related Accession (Same CDS sequence)	NM_004717.2 , NM_004717.3
Accession Version	NM_004717.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	diacylglycerol kinase iota
Comment

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGACA GATCTCAGGA ACATTTGCAC 
TTTGTGATGG AGATTTCCCA AGATGAGATT TTTATTCTGG ACCCAGATAT GGTGGTGTCA 
CAGCCGGCGG GGACACCTCC GGGCATGCCT GACCTGGTGG TGGAACAAGC CTCGGGGATC 
TCAGACTGGT GGAATCCTGC CCTGCGGAAA CGCATGCTGA GTGACAGTGG GCTGGGGATG 
ATAGCTCCCT ATTATGAGGA CTCAGATCTG AAAGATCTCA GCCACTCCCG CGTGCTACAG 
TCACCAGTCT CTTCAGAAGA TCATGCAATT TTGCAGGCAG TAATAGCTGG TGATCTTATG 
AAGCTAATAG AAAGCTATAA AAATGGAGGC AGTCTGCTAA TTCAGGGACC AGACCACTGT 
TCACTCCTTC ACTACGCAGC TAAAACCGGC AACGGGGAGA TTGTGAAATA TATCCTTGAC 
CACGGACCTT CCGAGTTATT GGATATGGCA GACAGTGAAA CGGGTGAGAC TGCACTGCAC 
AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG TGCCAGCTTC TGGTGGATGC AGGAGCATCT 
CTGAGAAAGA CGGACTCCAA GGGTAAGACA CCTCAAGAAA GAGCACAGCA GGCTGGGGAC 
CCAGACTTGG CTGCTTACCT AGAAAGCCGT CAGAACTATA AGGTCATTGG CCATGAGGAC 
CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_004708.1

Translation

MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASISDWLRTIAGELVQSFGAIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	NM_004717.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens diacylglycerol kinase, iota (DGKI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004717.2

ORF Insert Sequence:

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGACA GATCTCAGGA ACATTTGCAC 
TTTGTGATGG AGATTTCCCA AGATGAGATT TTTATTCTGG ACCCAGATAT GGTGGTGTCA 
CAGCCGGCGG GGACACCTCC GGGCATGCCT GACCTGGTGG TGGAACAAGC CTCGGGGATC 
TCAGACTGGT GGAATCCTGC CCTGCGGAAA CGCATGCTGA GTGACAGTGG GCTGGGGATG 
ATAGCTCCCT ATTATGAGGA CTCAGATCTG AAAGATCTCA GCCACTCCCG CGTGCTACAG 
TCACCAGTCT CTTCAGAAGA TCATGCAATT TTGCAGGCAG TAATAGCTGG TGATCTTATG 
AAGCTAATAG AAAGCTATAA AAATGGAGGC AGTCTGCTAA TTCAGGGACC AGACCACTGT 
TCACTCCTTC ACTACGCAGC TAAAACCGGC AACGGGGAGA TTGTGAAATA TATCCTTGAC 
CACGGACCTT CCGAGTTATT GGATATGGCA GACAGTGAAA CGGGTGAGAC TGCACTGCAC 
AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG TGCCAGCTTC TGGTGGATGC AGGAGCATCT 
CTGAGAAAGA CGGACTCCAA GGGTAAGACA CCTCAAGAAA GAGCACAGCA GGCTGGGGAC 
CCAGACTTGG CTGCTTACCT AGAAAGCCGT CAGAACTATA AGGTCATTGG CCATGAGGAC 
CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( NM_004717.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004708.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17163
Clone ID Related Accession (Same CDS sequence)	NM_004717.2 , NM_004717.3
Accession Version	NM_004717.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002813.1, AF061936.1, AC078842.2 and AW237608.1. On Mar 30, 2016 this sequence version replaced NM_004717.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2144120, SAMEA2145743 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGACA GATCTCAGGA ACATTTGCAC 
TTTGTGATGG AGATTTCCCA AGATGAGATT TTTATTCTGG ACCCAGATAT GGTGGTGTCA 
CAGCCGGCGG GGACACCTCC GGGCATGCCT GACCTGGTGG TGGAACAAGC CTCGGGGATC 
TCAGACTGGT GGAATCCTGC CCTGCGGAAA CGCATGCTGA GTGACAGTGG GCTGGGGATG 
ATAGCTCCCT ATTATGAGGA CTCAGATCTG AAAGATCTCA GCCACTCCCG CGTGCTACAG 
TCACCAGTCT CTTCAGAAGA TCATGCAATT TTGCAGGCAG TAATAGCTGG TGATCTTATG 
AAGCTAATAG AAAGCTATAA AAATGGAGGC AGTCTGCTAA TTCAGGGACC AGACCACTGT 
TCACTCCTTC ACTACGCAGC TAAAACCGGC AACGGGGAGA TTGTGAAATA TATCCTTGAC 
CACGGACCTT CCGAGTTATT GGATATGGCA GACAGTGAAA CGGGTGAGAC TGCACTGCAC 
AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG TGCCAGCTTC TGGTGGATGC AGGAGCATCT 
CTGAGAAAGA CGGACTCCAA GGGTAAGACA CCTCAAGAAA GAGCACAGCA GGCTGGGGAC 
CCAGACTTGG CTGCTTACCT AGAAAGCCGT CAGAACTATA AGGTCATTGG CCATGAGGAC 
CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004708.1
CDS	3..3200
Translation	MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASISDWLRTIAGELVQSFGAIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	NM_004717.3
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004717.3

ORF Insert Sequence:

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGACA GATCTCAGGA ACATTTGCAC 
TTTGTGATGG AGATTTCCCA AGATGAGATT TTTATTCTGG ACCCAGATAT GGTGGTGTCA 
CAGCCGGCGG GGACACCTCC GGGCATGCCT GACCTGGTGG TGGAACAAGC CTCGGGGATC 
TCAGACTGGT GGAATCCTGC CCTGCGGAAA CGCATGCTGA GTGACAGTGG GCTGGGGATG 
ATAGCTCCCT ATTATGAGGA CTCAGATCTG AAAGATCTCA GCCACTCCCG CGTGCTACAG 
TCACCAGTCT CTTCAGAAGA TCATGCAATT TTGCAGGCAG TAATAGCTGG TGATCTTATG 
AAGCTAATAG AAAGCTATAA AAATGGAGGC AGTCTGCTAA TTCAGGGACC AGACCACTGT 
TCACTCCTTC ACTACGCAGC TAAAACCGGC AACGGGGAGA TTGTGAAATA TATCCTTGAC 
CACGGACCTT CCGAGTTATT GGATATGGCA GACAGTGAAA CGGGTGAGAC TGCACTGCAC 
AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG TGCCAGCTTC TGGTGGATGC AGGAGCATCT 
CTGAGAAAGA CGGACTCCAA GGGTAAGACA CCTCAAGAAA GAGCACAGCA GGCTGGGGAC 
CCAGACTTGG CTGCTTACCT AGAAAGCCGT CAGAACTATA AGGTCATTGG CCATGAGGAC 
CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( NM_001321710.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308639.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 4, mRNA.

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CloneID	OHu101649
Clone ID Related Accession (Same CDS sequence)	NM_001321710.2 , NM_001321710.1
Accession Version	NM_001321710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-01
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002813.1, AB209167.1, AF061936.1, AC078842.2 and AW237608.1. On Jun 2, 2019 this sequence version replaced NM_001321710.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB209167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGCAA CTTCTGCTGA TCGCTTTTAT 
CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT 
TTTATTCTGG ACCCAGATAT GGTGGTGTCA CAGCCGGCGG GGACACCTCC GGGCATGCCT 
GACCTGGTGG TGGAACAAGC CTCGGGGTCA CCAGTCTCTT CAGAAGATCA TGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATAGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTAATTC AGGGACCAGA CCACTGTTCA CTCCTTCACT ACGCAGCTAA AACCGGCAAC 
GGGGAGATTG TGAAATATAT CCTTGACCAC GGACCTTCCG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACTGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACACCT 
CAAGAAAGAG CACAGCAGGC TGGGGACCCA GACTTGGCTG CTTACCTAGA AAGCCGTCAG 
AACTATAAGG TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308639.1
CDS	845..3049
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASISDWLRTIAGELVQSFGAIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	NM_001321710.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321710.2

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGCAA CTTCTGCTGA TCGCTTTTAT 
CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT 
TTTATTCTGG ACCCAGATAT GGTGGTGTCA CAGCCGGCGG GGACACCTCC GGGCATGCCT 
GACCTGGTGG TGGAACAAGC CTCGGGGTCA CCAGTCTCTT CAGAAGATCA TGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATAGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTAATTC AGGGACCAGA CCACTGTTCA CTCCTTCACT ACGCAGCTAA AACCGGCAAC 
GGGGAGATTG TGAAATATAT CCTTGACCAC GGACCTTCCG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACTGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACACCT 
CAAGAAAGAG CACAGCAGGC TGGGGACCCA GACTTGGCTG CTTACCTAGA AAGCCGTCAG 
AACTATAAGG TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( NM_001321710.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308639.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101649
Clone ID Related Accession (Same CDS sequence)	NM_001321710.2 , NM_001321710.1
Accession Version	NM_001321710.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002813.1, AB209167.1, AF061936.1, AC078842.2 and AW237608.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AB209167.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGCAA CTTCTGCTGA TCGCTTTTAT 
CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT 
TTTATTCTGG ACCCAGATAT GGTGGTGTCA CAGCCGGCGG GGACACCTCC GGGCATGCCT 
GACCTGGTGG TGGAACAAGC CTCGGGGTCA CCAGTCTCTT CAGAAGATCA TGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATAGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTAATTC AGGGACCAGA CCACTGTTCA CTCCTTCACT ACGCAGCTAA AACCGGCAAC 
GGGGAGATTG TGAAATATAT CCTTGACCAC GGACCTTCCG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACTGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACACCT 
CAAGAAAGAG CACAGCAGGC TGGGGACCCA GACTTGGCTG CTTACCTAGA AAGCCGTCAG 
AACTATAAGG TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308639.1
CDS	869..3073
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASISDWLRTIAGELVQSFGAIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	NM_001321710.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321710.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCT ATTTCAGACT GGTTAAGAAC CATTGCTGGG 
GAACTAGTGC AGTCATTTGG AGCGATACCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT 
GATTTGGAGA CTTGCCGTAT GTACATAGAC CGCCTACAGG AGGACCTACA GTCAGTTTCT 
TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCTCTCTCA 
GCACAGAGGC TCTCTCCTCG GTGGTGCTTC CTAGATGCAA CTTCTGCTGA TCGCTTTTAT 
CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT 
TTTATTCTGG ACCCAGATAT GGTGGTGTCA CAGCCGGCGG GGACACCTCC GGGCATGCCT 
GACCTGGTGG TGGAACAAGC CTCGGGGTCA CCAGTCTCTT CAGAAGATCA TGCAATTTTG 
CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATAGAAA GCTATAAAAA TGGAGGCAGT 
CTGCTAATTC AGGGACCAGA CCACTGTTCA CTCCTTCACT ACGCAGCTAA AACCGGCAAC 
GGGGAGATTG TGAAATATAT CCTTGACCAC GGACCTTCCG AGTTATTGGA TATGGCAGAC 
AGTGAAACGG GTGAGACTGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC 
CAGCTTCTGG TGGATGCAGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACACCT 
CAAGAAAGAG CACAGCAGGC TGGGGACCCA GACTTGGCTG CTTACCTAGA AAGCCGTCAG 
AACTATAAGG TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( NM_001321709.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308638.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 3, mRNA.

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CloneID	OHu97596
Clone ID Related Accession (Same CDS sequence)	NM_001321709.1 , XM_017012788.2 , XM_017012788.1
Accession Version	NM_001321709.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022173.7, AB209167.1, DR002813.1, AF061936.1, DA696180.1, AC078842.2 and AW237608.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.107799.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308638.1
CDS	681..2954
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	NM_001321709.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321709.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012785.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868274.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X7, mRNA.

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CloneID	OHu97594
Clone ID Related Accession (Same CDS sequence)	XM_017012785.1
Accession Version	XM_017012785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2640bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGA AATCAGCTCT CAGGAGGAAG TGTGCAGTCT GTAAAATCGT CGTCCACACC 
GCCTGCATTG AGCAGCTAGA AAAGATTAAT TTCAGATGTA AACCAACATT TCGAGAAGGA 
GGCTCAAGGT CACCAAGAGA AAATTTTGTA CGTCATCACT GGGTGCACAG GCGTCGGCAG 
GAGGGGAAAT GTAAGCAGTG TGGTAAGGGC TTCCAGCAAA AGTTCTCCTT CCACAGTAAA 
GAGATTGTGG CTATCAGCTG TTCCTGGTGC AAGCAGGCGT TTCACAATAA GGTGACCTGC 
TTCATGCTGC ATCACATTGA AGAACCCTGC TCCCTGGGGG CTCATGCTGC TGTTATTGTC 
CCGCCCACTT GGATCATTAA GGTGAAGAAA CCTCAGAACT CCCTGAAGGC TTCAAATCGG 
AAGAAGAAGA GAACAAGCTT TAAAAGAAAA GCCAGTAAAA GAGGGATGGA ACAGGAAAAC 
AAAGGTCGTC CTTTTGTGAT AAAACCCATC TCTTCTCCTC TCATGAAACC CTTGCTTGTA 
TTTGTGAATC CCAAGAGTGG AGGCAACCAG GGAACCAAAG TCCTGCAGAT GTTCATGTGG 
TACCTGAATC CACGGCAAGT CTTTGATCTT TCTCAGGAAG GGCCAAAAGA TGCGCTTGAA 
TTGTATAGGA AAGTACCAAA TCTGCGAATT CTGGCCTGTG GTGGGGATGG AACGGTGGGC 
TGGATCCTTT CCATCCTGGA TGAACTGCAG CTGAGCCCTC AGCCTCCTGT GGGGGTCCTT 
CCTCTGGGGA CTGGGAATGA CCTGGCTCGA ACTCTCAACT GGGGAGGGGG CTACACTGAT 
GAACCTGTTT CTAAGATCCT GTGTCAAGTG GAAGATGGGA CAGTTGTACA GCTAGATCGC 
TGGAACCTCC ATGTGGAAAG AAACCCCGAC TTGCCTCCAG AAGAACTTGA AGATGGCGTA 
TGTAAGCTCC CTCTGAATGT TTTCAATAAC TACTTCAGCC TTGGATTTGA TGCCCATGTC 
ACACTGGAGT TCCATGAATC CAGAGAAGCA AATCCAGAGA AATTCAACAG TCGTTTTCGA 
AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGACTTCC TACAGAGAAG TTCTAGAGAT 
CTATCCAAAC ATGTTAAAGT TGTTTGTGAT GGAACAGATC TCACCCCAAA GATTCAGGAA 
CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCC 
TGGGGAAACC CAGGTGATCA CCATGATTTC GAACCTCAGC GTCATGATGA TGGTTATATT 
GAAGTCATTG GATTTACCAT GGCCTCTTTG GCAGCCCTGC AAGTTGGGGG CCATGGAGAG 
AGGCTACACC AGTGTCGAGA AGTCATGCTT CTAACTTACA AATCCATCCC CATGCAAGTG 
GATGGGGAGC CCTGTAGGTT GGCCCCAGCT ATGATTCGGA TCTCCCTGAG GAATCAGGCC 
AACATGGTAC AGAAGAGCAA GAGGAGAACA TCCATGCCTT TACTCAATGA TATTCATCAG 
GTGCAAACTG CGGACCTGCG GCGAGTGTCT GCTCCCCCCG GCTCCTTCAC CATTCCCCAG 
TCTGTCCCAG ATCGTCTGAG GATCCGGGTG AACAAAATCA GTTTACAAGA CTATGAAGGA 
TTCCACTATG ACAAGGAGAA ACTCCGAGAA GCTTCGATAC CTCTGGGTAT TCTAGTTGTG 
CGTGGAGACT GTGATTTGGA GACTTGCCGT ATGTACATAG ACCGCCTACA GGAGGACCTA 
CAGTCAGTTT CTTCTGGCTC CCAGAGAGTT CATTACCAGG ACCATGAAAC CTCCTTCCCC 
AGGGCTCTCT CAGCACAGAG GCTCTCTCCT CGGTGGTGCT TCCTAGATGC AACTTCTGCT 
GATCGCTTTT ATCGAATAGA CAGATCTCAG GAACATTTGC ACTTTGTGAT GGAGATTTCC 
CAAGATGAGA TTTTTATTCT GGACCCAGAT ATGGTGGTGT CACAGCCGGC GGGGACACCT 
CCGGGCATGC CTGACCTGGT GGTGGAACAA GCCTCGGGGA TCTCAGACTG GTGGAATCCT 
GCCCTGCGGA AACGCATGCT GAGTGACAGT GGGCTGGGGA TGATAGCTCC CTATTATGAG 
GACTCAGATC TGAAAGATCT CAGCCACTCC CGCGTGCTAC AGTCACCAGT CTCTTCAGAA 
GATCATGCAA TTTTGCAGGC AGTAATAGCT GGTGATCTTA TGAAGCTAAT AGAAAGCTAT 
AAAAATGGAG GCAGTCTGCT AATTCAGGGA CCAGACCACT GTTCACTCCT TCACTACGCA 
GCTAAAACCG GCAACGGGGA GATTGTGAAA TATATCCTTG ACCACGGACC TTCCGAGTTA 
TTGGATATGG CAGACAGTGA AACGGGTGAG ACTGCACTGC ACAAGGCTGC CTGCCAGCGG 
AACCGGGCTG TGTGCCAGCT TCTGGTGGAT GCAGGAGCAT CTCTGAGAAA GACGGACTCC 
AAGGGTAAGA CACCTCAAGA AAGAGCACAG CAGGCTGGGG ACCCAGACTT GGCTGCTTAC 
CTAGAAAGCC GTCAGAACTA TAAGGTCATT GGCCATGAGG ACCTGGAAAC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868274.1
CDS	785..3424
Translation	MAAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012785.1

ORF Insert Sequence:

ATGGCAGCGA AATCAGCTCT CAGGAGGAAG TGTGCAGTCT GTAAAATCGT CGTCCACACC 
GCCTGCATTG AGCAGCTAGA AAAGATTAAT TTCAGATGTA AACCAACATT TCGAGAAGGA 
GGCTCAAGGT CACCAAGAGA AAATTTTGTA CGTCATCACT GGGTGCACAG GCGTCGGCAG 
GAGGGGAAAT GTAAGCAGTG TGGTAAGGGC TTCCAGCAAA AGTTCTCCTT CCACAGTAAA 
GAGATTGTGG CTATCAGCTG TTCCTGGTGC AAGCAGGCGT TTCACAATAA GGTGACCTGC 
TTCATGCTGC ATCACATTGA AGAACCCTGC TCCCTGGGGG CTCATGCTGC TGTTATTGTC 
CCGCCCACTT GGATCATTAA GGTGAAGAAA CCTCAGAACT CCCTGAAGGC TTCAAATCGG 
AAGAAGAAGA GAACAAGCTT TAAAAGAAAA GCCAGTAAAA GAGGGATGGA ACAGGAAAAC 
AAAGGTCGTC CTTTTGTGAT AAAACCCATC TCTTCTCCTC TCATGAAACC CTTGCTTGTA 
TTTGTGAATC CCAAGAGTGG AGGCAACCAG GGAACCAAAG TCCTGCAGAT GTTCATGTGG 
TACCTGAATC CACGGCAAGT CTTTGATCTT TCTCAGGAAG GGCCAAAAGA TGCGCTTGAA 
TTGTATAGGA AAGTACCAAA TCTGCGAATT CTGGCCTGTG GTGGGGATGG AACGGTGGGC 
TGGATCCTTT CCATCCTGGA TGAACTGCAG CTGAGCCCTC AGCCTCCTGT GGGGGTCCTT 
CCTCTGGGGA CTGGGAATGA CCTGGCTCGA ACTCTCAACT GGGGAGGGGG CTACACTGAT 
GAACCTGTTT CTAAGATCCT GTGTCAAGTG GAAGATGGGA CAGTTGTACA GCTAGATCGC 
TGGAACCTCC ATGTGGAAAG AAACCCCGAC TTGCCTCCAG AAGAACTTGA AGATGGCGTA 
TGTAAGCTCC CTCTGAATGT TTTCAATAAC TACTTCAGCC TTGGATTTGA TGCCCATGTC 
ACACTGGAGT TCCATGAATC CAGAGAAGCA AATCCAGAGA AATTCAACAG TCGTTTTCGA 
AATAAAATGT TCTATGCAGG GGCAGCTTTT TCTGACTTCC TACAGAGAAG TTCTAGAGAT 
CTATCCAAAC ATGTTAAAGT TGTTTGTGAT GGAACAGATC TCACCCCAAA GATTCAGGAA 
CTGAAGTTCC AGTGTATAGT ATTTTTAAAT ATACCCAGAT ATTGTGCTGG CACAATGCCC 
TGGGGAAACC CAGGTGATCA CCATGATTTC GAACCTCAGC GTCATGATGA TGGTTATATT 
GAAGTCATTG GATTTACCAT GGCCTCTTTG GCAGCCCTGC AAGTTGGGGG CCATGGAGAG 
AGGCTACACC AGTGTCGAGA AGTCATGCTT CTAACTTACA AATCCATCCC CATGCAAGTG 
GATGGGGAGC CCTGTAGGTT GGCCCCAGCT ATGATTCGGA TCTCCCTGAG GAATCAGGCC 
AACATGGTAC AGAAGAGCAA GAGGAGAACA TCCATGCCTT TACTCAATGA TATTCATCAG 
GTGCAAACTG CGGACCTGCG GCGAGTGTCT GCTCCCCCCG GCTCCTTCAC CATTCCCCAG 
TCTGTCCCAG ATCGTCTGAG GATCCGGGTG AACAAAATCA GTTTACAAGA CTATGAAGGA 
TTCCACTATG ACAAGGAGAA ACTCCGAGAA GCTTCGATAC CTCTGGGTAT TCTAGTTGTG 
CGTGGAGACT GTGATTTGGA GACTTGCCGT ATGTACATAG ACCGCCTACA GGAGGACCTA 
CAGTCAGTTT CTTCTGGCTC CCAGAGAGTT CATTACCAGG ACCATGAAAC CTCCTTCCCC 
AGGGCTCTCT CAGCACAGAG GCTCTCTCCT CGGTGGTGCT TCCTAGATGC AACTTCTGCT 
GATCGCTTTT ATCGAATAGA CAGATCTCAG GAACATTTGC ACTTTGTGAT GGAGATTTCC 
CAAGATGAGA TTTTTATTCT GGACCCAGAT ATGGTGGTGT CACAGCCGGC GGGGACACCT 
CCGGGCATGC CTGACCTGGT GGTGGAACAA GCCTCGGGGA TCTCAGACTG GTGGAATCCT 
GCCCTGCGGA AACGCATGCT GAGTGACAGT GGGCTGGGGA TGATAGCTCC CTATTATGAG 
GACTCAGATC TGAAAGATCT CAGCCACTCC CGCGTGCTAC AGTCACCAGT CTCTTCAGAA 
GATCATGCAA TTTTGCAGGC AGTAATAGCT GGTGATCTTA TGAAGCTAAT AGAAAGCTAT 
AAAAATGGAG GCAGTCTGCT AATTCAGGGA CCAGACCACT GTTCACTCCT TCACTACGCA 
GCTAAAACCG GCAACGGGGA GATTGTGAAA TATATCCTTG ACCACGGACC TTCCGAGTTA 
TTGGATATGG CAGACAGTGA AACGGGTGAG ACTGCACTGC ACAAGGCTGC CTGCCAGCGG 
AACCGGGCTG TGTGCCAGCT TCTGGTGGAT GCAGGAGCAT CTCTGAGAAA GACGGACTCC 
AAGGGTAAGA CACCTCAAGA AAGAGCACAG CAGGCTGGGG ACCCAGACTT GGCTGCTTAC 
CTAGAAAGCC GTCAGAACTA TAAGGTCATT GGCCATGAGG ACCTGGAAAC TGCTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012786.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868275.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu97595
Clone ID Related Accession (Same CDS sequence)	XM_017012786.1 , XM_017012787.1
Accession Version	XM_017012786.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868275.1
CDS	588..2924
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012786.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012786.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012787.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868276.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu97595
Clone ID Related Accession (Same CDS sequence)	XM_017012786.1 , XM_017012787.1
Accession Version	XM_017012787.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868276.1
CDS	453..2789
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012787.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012788.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868277.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu97596
Clone ID Related Accession (Same CDS sequence)	NM_001321709.1 , XM_017012788.2 , XM_017012788.1
Accession Version	XM_017012788.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012788.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868277.1
CDS	635..2908
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012788.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012788.2

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012788.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868277.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X10, mRNA.

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CloneID	OHu97596
Clone ID Related Accession (Same CDS sequence)	NM_001321709.1 , XM_017012788.2 , XM_017012788.1
Accession Version	XM_017012788.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868277.1
CDS	634..2907
Translation	MLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012788.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012788.1

ORF Insert Sequence:

ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_011516684.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514986.2 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X11, mRNA.

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CloneID	OHu97597
Clone ID Related Accession (Same CDS sequence)	XM_011516684.2
Accession Version	XM_011516684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAGG AAAACAAAGG TCGTCCTTTT GTGATAAAAC CCATCTCTTC TCCTCTCATG 
AAACCCTTGC TTGTATTTGT GAATCCCAAG AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG 
CAGATGTTCA TGTGGTACCT GAATCCACGG CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA 
AAAGATGCGC TTGAATTGTA TAGGAAAGTA CCAAATCTGC GAATTCTGGC CTGTGGTGGG 
GATGGAACGG TGGGCTGGAT CCTTTCCATC CTGGATGAAC TGCAGCTGAG CCCTCAGCCT 
CCTGTGGGGG TCCTTCCTCT GGGGACTGGG AATGACCTGG CTCGAACTCT CAACTGGGGA 
GGGGGCTACA CTGATGAACC TGTTTCTAAG ATCCTGTGTC AAGTGGAAGA TGGGACAGTT 
GTACAGCTAG ATCGCTGGAA CCTCCATGTG GAAAGAAACC CCGACTTGCC TCCAGAAGAA 
CTTGAAGATG GCGTATGTAA GCTCCCTCTG AATGTTTTCA ATAACTACTT CAGCCTTGGA 
TTTGATGCCC ATGTCACACT GGAGTTCCAT GAATCCAGAG AAGCAAATCC AGAGAAATTC 
AACAGTCGTT TTCGAAATAA AATGTTCTAT GCAGGGGCAG CTTTTTCTGA CTTCCTACAG 
AGAAGTTCTA GAGATCTATC CAAACATGTT AAAGTTGTTT GTGATGGAAC AGATCTCACC 
CCAAAGATTC AGGAACTGAA GTTCCAGTGT ATAGTATTTT TAAATATACC CAGATATTGT 
GCTGGCACAA TGCCCTGGGG AAACCCAGGT GATCACCATG ATTTCGAACC TCAGCGTCAT 
GATGATGGTT ATATTGAAGT CATTGGATTT ACCATGGCCT CTTTGGCAGC CCTGCAAGTT 
GGGGGCCATG GAGAGAGGCT ACACCAGTGT CGAGAAGTCA TGCTTCTAAC TTACAAATCC 
ATCCCCATGC AAGTGGATGG GGAGCCCTGT AGGTTGGCCC CAGCTATGAT TCGGATCTCC 
CTGAGGAATC AGGCCAACAT GGTACAGAAG AGCAAGAGGA GAACATCCAT GCCTTTACTC 
AATGATATTC ATCAGGTGCA AACTGCGGAC CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC 
TTCACCATTC CCCAGTCTGT CCCAGATCGT CTGAGGATCC GGGTGAACAA AATCAGTTTA 
CAAGACTATG AAGGATTCCA CTATGACAAG GAGAAACTCC GAGAAGCTTC GATACCTCTG 
GGTATTCTAG TTGTGCGTGG AGACTGTGAT TTGGAGACTT GCCGTATGTA CATAGACCGC 
CTACAGGAGG ACCTACAGTC AGTTTCTTCT GGCTCCCAGA GAGTTCATTA CCAGGACCAT 
GAAACCTCCT TCCCCAGGGC TCTCTCAGCA CAGAGGCTCT CTCCTCGGTG GTGCTTCCTA 
GATGCAACTT CTGCTGATCG CTTTTATCGA ATAGACAGAT CTCAGGAACA TTTGCACTTT 
GTGATGGAGA TTTCCCAAGA TGAGATTTTT ATTCTGGACC CAGATATGGT GGTGTCACAG 
CCGGCGGGGA CACCTCCGGG CATGCCTGAC CTGGTGGTGG AACAAGCCTC GGGGATCTCA 
GACTGGTGGA ATCCTGCCCT GCGGAAACGC ATGCTGAGTG ACAGTGGGCT GGGGATGATA 
GCTCCCTATT ATGAGGACTC AGATCTGAAA GATCTCAGCC ACTCCCGCGT GCTACAGTCA 
CCAGTCTCTT CAGAAGATCA TGCAATTTTG CAGGCAGTAA TAGCTGGTGA TCTTATGAAG 
CTAATAGAAA GCTATAAAAA TGGAGGCAGT CTGCTAATTC AGGGACCAGA CCACTGTTCA 
CTCCTTCACT ACGCAGCTAA AACCGGCAAC GGGGAGATTG TGAAATATAT CCTTGACCAC 
GGACCTTCCG AGTTATTGGA TATGGCAGAC AGTGAAACGG GTGAGACTGC ACTGCACAAG 
GCTGCCTGCC AGCGGAACCG GGCTGTGTGC CAGCTTCTGG TGGATGCAGG AGCATCTCTG 
AGAAAGACGG ACTCCAAGGG TAAGACACCT CAAGAAAGAG CACAGCAGGC TGGGGACCCA 
GACTTGGCTG CTTACCTAGA AAGCCGTCAG AACTATAAGG TCATTGGCCA TGAGGACCTG 
GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514986.2
CDS	8246..10420
Translation	MEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_011516684.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516684.2

ORF Insert Sequence:

ATGGAACAGG AAAACAAAGG TCGTCCTTTT GTGATAAAAC CCATCTCTTC TCCTCTCATG 
AAACCCTTGC TTGTATTTGT GAATCCCAAG AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG 
CAGATGTTCA TGTGGTACCT GAATCCACGG CAAGTCTTTG ATCTTTCTCA GGAAGGGCCA 
AAAGATGCGC TTGAATTGTA TAGGAAAGTA CCAAATCTGC GAATTCTGGC CTGTGGTGGG 
GATGGAACGG TGGGCTGGAT CCTTTCCATC CTGGATGAAC TGCAGCTGAG CCCTCAGCCT 
CCTGTGGGGG TCCTTCCTCT GGGGACTGGG AATGACCTGG CTCGAACTCT CAACTGGGGA 
GGGGGCTACA CTGATGAACC TGTTTCTAAG ATCCTGTGTC AAGTGGAAGA TGGGACAGTT 
GTACAGCTAG ATCGCTGGAA CCTCCATGTG GAAAGAAACC CCGACTTGCC TCCAGAAGAA 
CTTGAAGATG GCGTATGTAA GCTCCCTCTG AATGTTTTCA ATAACTACTT CAGCCTTGGA 
TTTGATGCCC ATGTCACACT GGAGTTCCAT GAATCCAGAG AAGCAAATCC AGAGAAATTC 
AACAGTCGTT TTCGAAATAA AATGTTCTAT GCAGGGGCAG CTTTTTCTGA CTTCCTACAG 
AGAAGTTCTA GAGATCTATC CAAACATGTT AAAGTTGTTT GTGATGGAAC AGATCTCACC 
CCAAAGATTC AGGAACTGAA GTTCCAGTGT ATAGTATTTT TAAATATACC CAGATATTGT 
GCTGGCACAA TGCCCTGGGG AAACCCAGGT GATCACCATG ATTTCGAACC TCAGCGTCAT 
GATGATGGTT ATATTGAAGT CATTGGATTT ACCATGGCCT CTTTGGCAGC CCTGCAAGTT 
GGGGGCCATG GAGAGAGGCT ACACCAGTGT CGAGAAGTCA TGCTTCTAAC TTACAAATCC 
ATCCCCATGC AAGTGGATGG GGAGCCCTGT AGGTTGGCCC CAGCTATGAT TCGGATCTCC 
CTGAGGAATC AGGCCAACAT GGTACAGAAG AGCAAGAGGA GAACATCCAT GCCTTTACTC 
AATGATATTC ATCAGGTGCA AACTGCGGAC CTGCGGCGAG TGTCTGCTCC CCCCGGCTCC 
TTCACCATTC CCCAGTCTGT CCCAGATCGT CTGAGGATCC GGGTGAACAA AATCAGTTTA 
CAAGACTATG AAGGATTCCA CTATGACAAG GAGAAACTCC GAGAAGCTTC GATACCTCTG 
GGTATTCTAG TTGTGCGTGG AGACTGTGAT TTGGAGACTT GCCGTATGTA CATAGACCGC 
CTACAGGAGG ACCTACAGTC AGTTTCTTCT GGCTCCCAGA GAGTTCATTA CCAGGACCAT 
GAAACCTCCT TCCCCAGGGC TCTCTCAGCA CAGAGGCTCT CTCCTCGGTG GTGCTTCCTA 
GATGCAACTT CTGCTGATCG CTTTTATCGA ATAGACAGAT CTCAGGAACA TTTGCACTTT 
GTGATGGAGA TTTCCCAAGA TGAGATTTTT ATTCTGGACC CAGATATGGT GGTGTCACAG 
CCGGCGGGGA CACCTCCGGG CATGCCTGAC CTGGTGGTGG AACAAGCCTC GGGGATCTCA 
GACTGGTGGA ATCCTGCCCT GCGGAAACGC ATGCTGAGTG ACAGTGGGCT GGGGATGATA 
GCTCCCTATT ATGAGGACTC AGATCTGAAA GATCTCAGCC ACTCCCGCGT GCTACAGTCA 
CCAGTCTCTT CAGAAGATCA TGCAATTTTG CAGGCAGTAA TAGCTGGTGA TCTTATGAAG 
CTAATAGAAA GCTATAAAAA TGGAGGCAGT CTGCTAATTC AGGGACCAGA CCACTGTTCA 
CTCCTTCACT ACGCAGCTAA AACCGGCAAC GGGGAGATTG TGAAATATAT CCTTGACCAC 
GGACCTTCCG AGTTATTGGA TATGGCAGAC AGTGAAACGG GTGAGACTGC ACTGCACAAG 
GCTGCCTGCC AGCGGAACCG GGCTGTGTGC CAGCTTCTGG TGGATGCAGG AGCATCTCTG 
AGAAAGACGG ACTCCAAGGG TAAGACACCT CAAGAAAGAG CACAGCAGGC TGGGGACCCA 
GACTTGGCTG CTTACCTAGA AAGCCGTCAG AACTATAAGG TCATTGGCCA TGAGGACCTG 
GAAACTGCTG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012789.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868278.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X12, mRNA.

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CloneID	OHu97598
Clone ID Related Accession (Same CDS sequence)	XM_017012789.1
Accession Version	XM_017012789.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTATG CAGGGGCAGC TTTTTCTGAC TTCCTACAGA GAAGTTCTAG AGATCTATCC 
AAACATGTTA AAGTTGTTTG TGATGGAACA GATCTCACCC CAAAGATTCA GGAACTGAAG 
TTCCAGTGTA TAGTATTTTT AAATATACCC AGATATTGTG CTGGCACAAT GCCCTGGGGA 
AACCCAGGTG ATCACCATGA TTTCGAACCT CAGCGTCATG ATGATGGTTA TATTGAAGTC 
ATTGGATTTA CCATGGCCTC TTTGGCAGCC CTGCAAGTTG GGGGCCATGG AGAGAGGCTA 
CACCAGTGTC GAGAAGTCAT GCTTCTAACT TACAAATCCA TCCCCATGCA AGTGGATGGG 
GAGCCCTGTA GGTTGGCCCC AGCTATGATT CGGATCTCCC TGAGGAATCA GGCCAACATG 
GTACAGAAGA GCAAGAGGAG AACATCCATG CCTTTACTCA ATGATATTCA TCAGGTGCAA 
ACTGCGGACC TGCGGCGAGT GTCTGCTCCC CCCGGCTCCT TCACCATTCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868278.1
CDS	80..1633
Translation	MFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012789.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012789.1

ORF Insert Sequence:

ATGTTCTATG CAGGGGCAGC TTTTTCTGAC TTCCTACAGA GAAGTTCTAG AGATCTATCC 
AAACATGTTA AAGTTGTTTG TGATGGAACA GATCTCACCC CAAAGATTCA GGAACTGAAG 
TTCCAGTGTA TAGTATTTTT AAATATACCC AGATATTGTG CTGGCACAAT GCCCTGGGGA 
AACCCAGGTG ATCACCATGA TTTCGAACCT CAGCGTCATG ATGATGGTTA TATTGAAGTC 
ATTGGATTTA CCATGGCCTC TTTGGCAGCC CTGCAAGTTG GGGGCCATGG AGAGAGGCTA 
CACCAGTGTC GAGAAGTCAT GCTTCTAACT TACAAATCCA TCCCCATGCA AGTGGATGGG 
GAGCCCTGTA GGTTGGCCCC AGCTATGATT CGGATCTCCC TGAGGAATCA GGCCAACATG 
GTACAGAAGA GCAAGAGGAG AACATCCATG CCTTTACTCA ATGATATTCA TCAGGTGCAA 
ACTGCGGACC TGCGGCGAGT GTCTGCTCCC CCCGGCTCCT TCACCATTCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( NM_001321708.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001308637.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 2, mRNA.

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CloneID	OHu101847
Clone ID Related Accession (Same CDS sequence)	NM_001321708.1
Accession Version	NM_001321708.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3174bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DR002813.1, AF061936.1, DA696180.1, AC078842.2 and AW237608.1. On Mar 30, 2016 this sequence version replaced XM_006716172.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.116607.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001308637.1
CDS	3..3176
Translation	MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	NM_001321708.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001321708.1

ORF Insert Sequence:

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAGTCTGTC 
CCAGATCGTC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC 
TATGACAAGG AGAAACTCCG AGAAGCTTCG ATACCTCTGG GTATTCTAGT TGTGCGTGGA 
GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGACCGCC TACAGGAGGA CCTACAGTCA 
GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCT 
CTCTCAGCAC AGAGGCTCTC TCCTCGGTGG TGCTTCCTAG ATGCAACTTC TGCTGATCGC 
TTTTATCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT 
GAGATTTTTA TTCTGGACCC AGATATGGTG GTGTCACAGC CGGCGGGGAC ACCTCCGGGC 
ATGCCTGACC TGGTGGTGGA ACAAGCCTCG GGGATCTCAG ACTGGTGGAA TCCTGCCCTG 
CGGAAACGCA TGCTGAGTGA CAGTGGGCTG GGGATGATAG CTCCCTATTA TGAGGACTCA 
GATCTGAAAG ATCTCAGCCA CTCCCGCGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAT 
GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATAGAAAG CTATAAAAAT 
GGAGGCAGTC TGCTAATTCA GGGACCAGAC CACTGTTCAC TCCTTCACTA CGCAGCTAAA 
ACCGGCAACG GGGAGATTGT GAAATATATC CTTGACCACG GACCTTCCGA GTTATTGGAT 
ATGGCAGACA GTGAAACGGG TGAGACTGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG 
GCTGTGTGCC AGCTTCTGGT GGATGCAGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT 
AAGACACCTC AAGAAAGAGC ACAGCAGGCT GGGGACCCAG ACTTGGCTGC TTACCTAGAA 
AGCCGTCAGA ACTATAAGGT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012780.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868269.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97588
Clone ID Related Accession (Same CDS sequence)	XM_017012780.1 , XM_017012780.2
Accession Version	XM_017012780.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868269.1
CDS	1..3237
Translation	MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012780.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012780.1

ORF Insert Sequence:

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012780.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868269.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu97588
Clone ID Related Accession (Same CDS sequence)	XM_017012780.1 , XM_017012780.2
Accession Version	XM_017012780.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017012780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868269.1
CDS	1..3237
Translation	MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012780.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012780.2

ORF Insert Sequence:

ATGGATGCTG CGGGAAGGGG CTGCCATTTG CTGCCCCTGC CAGCGGCGCG CGGACCTGCC 
CGCGCTCCTG CAGCCGCCGC CGCCGCCGCC GCCAGCCCGC CCGGCCCCTG CAGCGGCGCC 
GCCTGCGCTC CCTCCGCGGC CGCCGGAGCG GGCGCCATGA ACCCCAGCTC CTCGGCGGGA 
GAGGAGAAAG GGGCGACGGG CGGCAGCAGC AGCAGCGGAA GCGGCGCCGG GAGCTGCTGC 
CTGGGCGCCG AGGGCGGCGC GGACCCGCGG GGCGCAGGGT CAGCCGCGGC GGCGGGGGCC 
GCTGCCCTGG ACGAGCCCGC GGCCGCCGGC CAGAAGGAGA AGGACGAAGC GCTGGAGGAG 
AAGCTGAGGA ACTTAACTTT CCGGAAGCAG GTCTCGTACA GGAAAGCAAT CTCCCGGGCA 
GGCCTCCAGC ATCTGGCTCC TGCACATCCC CTCAGCCTTC CTGTGGCAAA TGGTCCAGCC 
AAGGAGCCCA GAGCGACTTT GGACTGGAGT GAGAATGCCG TGAATGGAGA ACACCTGTGG 
CTGGAGACCA ACGTCTCGGG AGACCTCTGC TACCTTGGAG AGGAGAACTG CCAAGTCAGA 
TTTGCAAAAT CAGCTCTCAG GAGGAAGTGT GCAGTCTGTA AAATCGTCGT CCACACCGCC 
TGCATTGAGC AGCTAGAAAA GATTAATTTC AGATGTAAAC CAACATTTCG AGAAGGAGGC 
TCAAGGTCAC CAAGAGAAAA TTTTGTACGT CATCACTGGG TGCACAGGCG TCGGCAGGAG 
GGGAAATGTA AGCAGTGTGG TAAGGGCTTC CAGCAAAAGT TCTCCTTCCA CAGTAAAGAG 
ATTGTGGCTA TCAGCTGTTC CTGGTGCAAG CAGGCGTTTC ACAATAAGGT GACCTGCTTC 
ATGCTGCATC ACATTGAAGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATTGTCCCG 
CCCACTTGGA TCATTAAGGT GAAGAAACCT CAGAACTCCC TGAAGGCTTC AAATCGGAAG 
AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GGATGGAACA GGAAAACAAA 
GGTCGTCCTT TTGTGATAAA ACCCATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTATTT 
GTGAATCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC 
CTGAATCCAC GGCAAGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAATTG 
TATAGGAAAG TACCAAATCT GCGAATTCTG GCCTGTGGTG GGGATGGAAC GGTGGGCTGG 
ATCCTTTCCA TCCTGGATGA ACTGCAGCTG AGCCCTCAGC CTCCTGTGGG GGTCCTTCCT 
CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA 
CCTGTTTCTA AGATCCTGTG TCAAGTGGAA GATGGGACAG TTGTACAGCT AGATCGCTGG 
AACCTCCATG TGGAAAGAAA CCCCGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT 
AAGCTCCCTC TGAATGTTTT CAATAACTAC TTCAGCCTTG GATTTGATGC CCATGTCACA 
CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT 
AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GACTTCCTAC AGAGAAGTTC TAGAGATCTA 
TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CCCCAAAGAT TCAGGAACTG 
AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG 
GGAAACCCAG GTGATCACCA TGATTTCGAA CCTCAGCGTC ATGATGATGG TTATATTGAA 
GTCATTGGAT TTACCATGGC CTCTTTGGCA GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG 
CTACACCAGT GTCGAGAAGT CATGCTTCTA ACTTACAAAT CCATCCCCAT GCAAGTGGAT 
GGGGAGCCCT GTAGGTTGGC CCCAGCTATG ATTCGGATCT CCCTGAGGAA TCAGGCCAAC 
ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT TCATCAGGTG 
CAAACTGCGG ACCTGCGGCG AGTGTCTGCT CCCCCCGGCT CCTTCACCAT TCCCCAGTCT 
GTCCCAGATC GTCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC 
CACTATGACA AGGAGAAACT CCGAGAAGCT TCGATACCTC TGGGTATTCT AGTTGTGCGT 
GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGACC GCCTACAGGA GGACCTACAG 
TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG 
GCTCTCTCAG CACAGAGGCT CTCTCCTCGG TGGTGCTTCC TAGATGCAAC TTCTGCTGAT 
CGCTTTTATC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA 
GATGAGATTT TTATTCTGGA CCCAGATATG GTGGTGTCAC AGCCGGCGGG GACACCTCCG 
GGCATGCCTG ACCTGGTGGT GGAACAAGCC TCGGGGATCT CAGACTGGTG GAATCCTGCC 
CTGCGGAAAC GCATGCTGAG TGACAGTGGG CTGGGGATGA TAGCTCCCTA TTATGAGGAC 
TCAGATCTGA AAGATCTCAG CCACTCCCGC GTGCTACAGT CACCAGTCTC TTCAGAAGAT 
CATGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATAGA AAGCTATAAA 
AATGGAGGCA GTCTGCTAAT TCAGGGACCA GACCACTGTT CACTCCTTCA CTACGCAGCT 
AAAACCGGCA ACGGGGAGAT TGTGAAATAT ATCCTTGACC ACGGACCTTC CGAGTTATTG 
GATATGGCAG ACAGTGAAAC GGGTGAGACT GCACTGCACA AGGCTGCCTG CCAGCGGAAC 
CGGGCTGTGT GCCAGCTTCT GGTGGATGCA GGAGCATCTC TGAGAAAGAC GGACTCCAAG 
GGTAAGACAC CTCAAGAAAG AGCACAGCAG GCTGGGGACC CAGACTTGGC TGCTTACCTA 
GAAAGCCGTC AGAACTATAA GGTCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012781.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868270.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu97589
Clone ID Related Accession (Same CDS sequence)	XM_017012781.1
Accession Version	XM_017012781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGAACTCCCT GAAGGCTTCA 
AATCGGAAGA AGAAGAGAAC AAGCTTTAAA AGAAAAGCCA GTAAAAGAGG GATGGAACAG 
GAAAACAAAG GTCGTCCTTT TGTGATAAAA CCCATCTCTT CTCCTCTCAT GAAACCCTTG 
CTTGTATTTG TGAATCCCAA GAGTGGAGGC AACCAGGGAA CCAAAGTCCT GCAGATGTTC 
ATGTGGTACC TGAATCCACG GCAAGTCTTT GATCTTTCTC AGGAAGGGCC AAAAGATGCG 
CTTGAATTGT ATAGGAAAGT ACCAAATCTG CGAATTCTGG CCTGTGGTGG GGATGGAACG 
GTGGGCTGGA TCCTTTCCAT CCTGGATGAA CTGCAGCTGA GCCCTCAGCC TCCTGTGGGG 
GTCCTTCCTC TGGGGACTGG GAATGACCTG GCTCGAACTC TCAACTGGGG AGGGGGCTAC 
ACTGATGAAC CTGTTTCTAA GATCCTGTGT CAAGTGGAAG ATGGGACAGT TGTACAGCTA 
GATCGCTGGA ACCTCCATGT GGAAAGAAAC CCCGACTTGC CTCCAGAAGA ACTTGAAGAT 
GGCGTATGTA AGCTCCCTCT GAATGTTTTC AATAACTACT TCAGCCTTGG ATTTGATGCC 
CATGTCACAC TGGAGTTCCA TGAATCCAGA GAAGCAAATC CAGAGAAATT CAACAGTCGT 
TTTCGAAATA AAATGTTCTA TGCAGGGGCA GCTTTTTCTG ACTTCCTACA GAGAAGTTCT 
AGAGATCTAT CCAAACATGT TAAAGTTGTT TGTGATGGAA CAGATCTCAC CCCAAAGATT 
CAGGAACTGA AGTTCCAGTG TATAGTATTT TTAAATATAC CCAGATATTG TGCTGGCACA 
ATGCCCTGGG GAAACCCAGG TGATCACCAT GATTTCGAAC CTCAGCGTCA TGATGATGGT 
TATATTGAAG TCATTGGATT TACCATGGCC TCTTTGGCAG CCCTGCAAGT TGGGGGCCAT 
GGAGAGAGGC TACACCAGTG TCGAGAAGTC ATGCTTCTAA CTTACAAATC CATCCCCATG 
CAAGTGGATG GGGAGCCCTG TAGGTTGGCC CCAGCTATGA TTCGGATCTC CCTGAGGAAT 
CAGGCCAACA TGGTACAGAA GAGCAAGAGG AGAACATCCA TGCCTTTACT CAATGATATT 
CATCAGGTGC AAACTGCGGA CCTGCGGCGA GTGTCTGCTC CCCCCGGCTC CTTCACCATT 
CCCCAGTCTG TCCCAGATCG TCTGAGGATC CGGGTGAACA AAATCAGTTT ACAAGACTAT 
GAAGGATTCC ACTATGACAA GGAGAAACTC CGAGAAGCTT CGATACCTCT GGGTATTCTA 
GTTGTGCGTG GAGACTGTGA TTTGGAGACT TGCCGTATGT ACATAGACCG CCTACAGGAG 
GACCTACAGT CAGTTTCTTC TGGCTCCCAG AGAGTTCATT ACCAGGACCA TGAAACCTCC 
TTCCCCAGGG CTCTCTCAGC ACAGAGGCTC TCTCCTCGGT GGTGCTTCCT AGATGCAACT 
TCTGCTGATC GCTTTTATCG AATAGACAGA TCTCAGGAAC ATTTGCACTT TGTGATGGAG 
ATTTCCCAAG ATGAGATTTT TATTCTGGAC CCAGATATGG TGGTGTCACA GCCGGCGGGG 
ACACCTCCGG GCATGCCTGA CCTGGTGGTG GAACAAGCCT CGGGGATCTC AGACTGGTGG 
AATCCTGCCC TGCGGAAACG CATGCTGAGT GACAGTGGGC TGGGGATGAT AGCTCCCTAT 
TATGAGGACT CAGATCTGAA AGATCTCAGC CACTCCCGCG TGCTACAGTC ACCAGTCTCT 
TCAGAAGATC ATGCAATTTT GCAGGCAGTA ATAGCTGGTG ATCTTATGAA GCTAATAGAA 
AGCTATAAAA ATGGAGGCAG TCTGCTAATT CAGGGACCAG ACCACTGTTC ACTCCTTCAC 
TACGCAGCTA AAACCGGCAA CGGGGAGATT GTGAAATATA TCCTTGACCA CGGACCTTCC 
GAGTTATTGG ATATGGCAGA CAGTGAAACG GGTGAGACTG CACTGCACAA GGCTGCCTGC 
CAGCGGAACC GGGCTGTGTG CCAGCTTCTG GTGGATGCAG GAGCATCTCT GAGAAAGACG 
GACTCCAAGG GTAAGACACC TCAAGAAAGA GCACAGCAGG CTGGGGACCC AGACTTGGCT 
GCTTACCTAG AAAGCCGTCA GAACTATAAG GTCATTGGCC ATGAGGACCT GGAAACTGCT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868270.1
CDS	156..3221
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012781.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGAACTCCCT GAAGGCTTCA 
AATCGGAAGA AGAAGAGAAC AAGCTTTAAA AGAAAAGCCA GTAAAAGAGG GATGGAACAG 
GAAAACAAAG GTCGTCCTTT TGTGATAAAA CCCATCTCTT CTCCTCTCAT GAAACCCTTG 
CTTGTATTTG TGAATCCCAA GAGTGGAGGC AACCAGGGAA CCAAAGTCCT GCAGATGTTC 
ATGTGGTACC TGAATCCACG GCAAGTCTTT GATCTTTCTC AGGAAGGGCC AAAAGATGCG 
CTTGAATTGT ATAGGAAAGT ACCAAATCTG CGAATTCTGG CCTGTGGTGG GGATGGAACG 
GTGGGCTGGA TCCTTTCCAT CCTGGATGAA CTGCAGCTGA GCCCTCAGCC TCCTGTGGGG 
GTCCTTCCTC TGGGGACTGG GAATGACCTG GCTCGAACTC TCAACTGGGG AGGGGGCTAC 
ACTGATGAAC CTGTTTCTAA GATCCTGTGT CAAGTGGAAG ATGGGACAGT TGTACAGCTA 
GATCGCTGGA ACCTCCATGT GGAAAGAAAC CCCGACTTGC CTCCAGAAGA ACTTGAAGAT 
GGCGTATGTA AGCTCCCTCT GAATGTTTTC AATAACTACT TCAGCCTTGG ATTTGATGCC 
CATGTCACAC TGGAGTTCCA TGAATCCAGA GAAGCAAATC CAGAGAAATT CAACAGTCGT 
TTTCGAAATA AAATGTTCTA TGCAGGGGCA GCTTTTTCTG ACTTCCTACA GAGAAGTTCT 
AGAGATCTAT CCAAACATGT TAAAGTTGTT TGTGATGGAA CAGATCTCAC CCCAAAGATT 
CAGGAACTGA AGTTCCAGTG TATAGTATTT TTAAATATAC CCAGATATTG TGCTGGCACA 
ATGCCCTGGG GAAACCCAGG TGATCACCAT GATTTCGAAC CTCAGCGTCA TGATGATGGT 
TATATTGAAG TCATTGGATT TACCATGGCC TCTTTGGCAG CCCTGCAAGT TGGGGGCCAT 
GGAGAGAGGC TACACCAGTG TCGAGAAGTC ATGCTTCTAA CTTACAAATC CATCCCCATG 
CAAGTGGATG GGGAGCCCTG TAGGTTGGCC CCAGCTATGA TTCGGATCTC CCTGAGGAAT 
CAGGCCAACA TGGTACAGAA GAGCAAGAGG AGAACATCCA TGCCTTTACT CAATGATATT 
CATCAGGTGC AAACTGCGGA CCTGCGGCGA GTGTCTGCTC CCCCCGGCTC CTTCACCATT 
CCCCAGTCTG TCCCAGATCG TCTGAGGATC CGGGTGAACA AAATCAGTTT ACAAGACTAT 
GAAGGATTCC ACTATGACAA GGAGAAACTC CGAGAAGCTT CGATACCTCT GGGTATTCTA 
GTTGTGCGTG GAGACTGTGA TTTGGAGACT TGCCGTATGT ACATAGACCG CCTACAGGAG 
GACCTACAGT CAGTTTCTTC TGGCTCCCAG AGAGTTCATT ACCAGGACCA TGAAACCTCC 
TTCCCCAGGG CTCTCTCAGC ACAGAGGCTC TCTCCTCGGT GGTGCTTCCT AGATGCAACT 
TCTGCTGATC GCTTTTATCG AATAGACAGA TCTCAGGAAC ATTTGCACTT TGTGATGGAG 
ATTTCCCAAG ATGAGATTTT TATTCTGGAC CCAGATATGG TGGTGTCACA GCCGGCGGGG 
ACACCTCCGG GCATGCCTGA CCTGGTGGTG GAACAAGCCT CGGGGATCTC AGACTGGTGG 
AATCCTGCCC TGCGGAAACG CATGCTGAGT GACAGTGGGC TGGGGATGAT AGCTCCCTAT 
TATGAGGACT CAGATCTGAA AGATCTCAGC CACTCCCGCG TGCTACAGTC ACCAGTCTCT 
TCAGAAGATC ATGCAATTTT GCAGGCAGTA ATAGCTGGTG ATCTTATGAA GCTAATAGAA 
AGCTATAAAA ATGGAGGCAG TCTGCTAATT CAGGGACCAG ACCACTGTTC ACTCCTTCAC 
TACGCAGCTA AAACCGGCAA CGGGGAGATT GTGAAATATA TCCTTGACCA CGGACCTTCC 
GAGTTATTGG ATATGGCAGA CAGTGAAACG GGTGAGACTG CACTGCACAA GGCTGCCTGC 
CAGCGGAACC GGGCTGTGTG CCAGCTTCTG GTGGATGCAG GAGCATCTCT GAGAAAGACG 
GACTCCAAGG GTAAGACACC TCAAGAAAGA GCACAGCAGG CTGGGGACCC AGACTTGGCT 
GCTTACCTAG AAAGCCGTCA GAACTATAAG GTCATTGGCC ATGAGGACCT GGAAACTGCT 
GTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012782.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868271.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu97590
Clone ID Related Accession (Same CDS sequence)	XM_017012782.1
Accession Version	XM_017012782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGGTGAAGAA ACCTCAGAAC 
TCCCTGAAGG CTTCAAATCG GAAGAAGAAG AGAACAAGCT TTAAAAGAAA AGCCAGTAAA 
AGAGGGATGG AACAGGAAAA CAAAGGTCGT CCTTTTGTGA TAAAACCCAT CTCTTCTCCT 
CTCATGAAAC CCTTGCTTGT ATTTGTGAAT CCCAAGAGTG GAGGCAACCA GGGAACCAAA 
GTCCTGCAGA TGTTCATGTG GTACCTGAAT CCACGGCAAG TCTTTGATCT TTCTCAGGAA 
GGGCCAAAAG ATGCGCTTGA ATTGTATAGG AAAGTACCAA ATCTGCGAAT TCTGGCCTGT 
GGTGGGGATG GAACGGGCTA CACTGATGAA CCTGTTTCTA AGATCCTGTG TCAAGTGGAA 
GATGGGACAG TTGTACAGCT AGATCGCTGG AACCTCCATG TGGAAAGAAA CCCCGACTTG 
CCTCCAGAAG AACTTGAAGA TGGCGTATGT AAGCTCCCTC TGAATGTTTT CAATAACTAC 
TTCAGCCTTG GATTTGATGC CCATGTCACA CTGGAGTTCC ATGAATCCAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCTTTTTCT 
GACTTCCTAC AGAGAAGTTC TAGAGATCTA TCCAAACATG TTAAAGTTGT TTGTGATGGA 
ACAGATCTCA CCCCAAAGAT TCAGGAACTG AAGTTCCAGT GTATAGTATT TTTAAATATA 
CCCAGATATT GTGCTGGCAC AATGCCCTGG GGAAACCCAG GTGATCACCA TGATTTCGAA 
CCTCAGCGTC ATGATGATGG TTATATTGAA GTCATTGGAT TTACCATGGC CTCTTTGGCA 
GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG CTACACCAGT GTCGAGAAGT CATGCTTCTA 
ACTTACAAAT CCATCCCCAT GCAAGTGGAT GGGGAGCCCT GTAGGTTGGC CCCAGCTATG 
ATTCGGATCT CCCTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTAC TCAATGATAT TCATCAGGTG CAAACTGCGG ACCTGCGGCG AGTGTCTGCT 
CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GTCTGAGGAT CCGGGTGAAC 
AAAATCAGTT TACAAGACTA TGAAGGATTC CACTATGACA AGGAGAAACT CCGAGAAGCT 
TCGATACCTC TGGGTATTCT AGTTGTGCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG 
TACATAGACC GCCTACAGGA GGACCTACAG TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT 
TACCAGGACC ATGAAACCTC CTTCCCCAGG GCTCTCTCAG CACAGAGGCT CTCTCCTCGG 
TGGTGCTTCC TAGATGCAAC TTCTGCTGAT CGCTTTTATC GAATAGACAG ATCTCAGGAA 
CATTTGCACT TTGTGATGGA GATTTCCCAA GATGAGATTT TTATTCTGGA CCCAGATATG 
GTGGTGTCAC AGCCGGCGGG GACACCTCCG GGCATGCCTG ACCTGGTGGT GGAACAAGCC 
TCGGGGATCT CAGACTGGTG GAATCCTGCC CTGCGGAAAC GCATGCTGAG TGACAGTGGG 
CTGGGGATGA TAGCTCCCTA TTATGAGGAC TCAGATCTGA AAGATCTCAG CCACTCCCGC 
GTGCTACAGT CACCAGTCTC TTCAGAAGAT CATGCAATTT TGCAGGCAGT AATAGCTGGT 
GATCTTATGA AGCTAATAGA AAGCTATAAA AATGGAGGCA GTCTGCTAAT TCAGGGACCA 
GACCACTGTT CACTCCTTCA CTACGCAGCT AAAACCGGCA ACGGGGAGAT TGTGAAATAT 
ATCCTTGACC ACGGACCTTC CGAGTTATTG GATATGGCAG ACAGTGAAAC GGGTGAGACT 
GCACTGCACA AGGCTGCCTG CCAGCGGAAC CGGGCTGTGT GCCAGCTTCT GGTGGATGCA 
GGAGCATCTC TGAGAAAGAC GGACTCCAAG GGTAAGACAC CTCAAGAAAG AGCACAGCAG 
GCTGGGGACC CAGACTTGGC TGCTTACCTA GAAAGCCGTC AGAACTATAA GGTCATTGGC 
CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868271.1
CDS	156..3122
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012782.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGGTGAAGAA ACCTCAGAAC 
TCCCTGAAGG CTTCAAATCG GAAGAAGAAG AGAACAAGCT TTAAAAGAAA AGCCAGTAAA 
AGAGGGATGG AACAGGAAAA CAAAGGTCGT CCTTTTGTGA TAAAACCCAT CTCTTCTCCT 
CTCATGAAAC CCTTGCTTGT ATTTGTGAAT CCCAAGAGTG GAGGCAACCA GGGAACCAAA 
GTCCTGCAGA TGTTCATGTG GTACCTGAAT CCACGGCAAG TCTTTGATCT TTCTCAGGAA 
GGGCCAAAAG ATGCGCTTGA ATTGTATAGG AAAGTACCAA ATCTGCGAAT TCTGGCCTGT 
GGTGGGGATG GAACGGGCTA CACTGATGAA CCTGTTTCTA AGATCCTGTG TCAAGTGGAA 
GATGGGACAG TTGTACAGCT AGATCGCTGG AACCTCCATG TGGAAAGAAA CCCCGACTTG 
CCTCCAGAAG AACTTGAAGA TGGCGTATGT AAGCTCCCTC TGAATGTTTT CAATAACTAC 
TTCAGCCTTG GATTTGATGC CCATGTCACA CTGGAGTTCC ATGAATCCAG AGAAGCAAAT 
CCAGAGAAAT TCAACAGTCG TTTTCGAAAT AAAATGTTCT ATGCAGGGGC AGCTTTTTCT 
GACTTCCTAC AGAGAAGTTC TAGAGATCTA TCCAAACATG TTAAAGTTGT TTGTGATGGA 
ACAGATCTCA CCCCAAAGAT TCAGGAACTG AAGTTCCAGT GTATAGTATT TTTAAATATA 
CCCAGATATT GTGCTGGCAC AATGCCCTGG GGAAACCCAG GTGATCACCA TGATTTCGAA 
CCTCAGCGTC ATGATGATGG TTATATTGAA GTCATTGGAT TTACCATGGC CTCTTTGGCA 
GCCCTGCAAG TTGGGGGCCA TGGAGAGAGG CTACACCAGT GTCGAGAAGT CATGCTTCTA 
ACTTACAAAT CCATCCCCAT GCAAGTGGAT GGGGAGCCCT GTAGGTTGGC CCCAGCTATG 
ATTCGGATCT CCCTGAGGAA TCAGGCCAAC ATGGTACAGA AGAGCAAGAG GAGAACATCC 
ATGCCTTTAC TCAATGATAT TCATCAGGTG CAAACTGCGG ACCTGCGGCG AGTGTCTGCT 
CCCCCCGGCT CCTTCACCAT TCCCCAGTCT GTCCCAGATC GTCTGAGGAT CCGGGTGAAC 
AAAATCAGTT TACAAGACTA TGAAGGATTC CACTATGACA AGGAGAAACT CCGAGAAGCT 
TCGATACCTC TGGGTATTCT AGTTGTGCGT GGAGACTGTG ATTTGGAGAC TTGCCGTATG 
TACATAGACC GCCTACAGGA GGACCTACAG TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT 
TACCAGGACC ATGAAACCTC CTTCCCCAGG GCTCTCTCAG CACAGAGGCT CTCTCCTCGG 
TGGTGCTTCC TAGATGCAAC TTCTGCTGAT CGCTTTTATC GAATAGACAG ATCTCAGGAA 
CATTTGCACT TTGTGATGGA GATTTCCCAA GATGAGATTT TTATTCTGGA CCCAGATATG 
GTGGTGTCAC AGCCGGCGGG GACACCTCCG GGCATGCCTG ACCTGGTGGT GGAACAAGCC 
TCGGGGATCT CAGACTGGTG GAATCCTGCC CTGCGGAAAC GCATGCTGAG TGACAGTGGG 
CTGGGGATGA TAGCTCCCTA TTATGAGGAC TCAGATCTGA AAGATCTCAG CCACTCCCGC 
GTGCTACAGT CACCAGTCTC TTCAGAAGAT CATGCAATTT TGCAGGCAGT AATAGCTGGT 
GATCTTATGA AGCTAATAGA AAGCTATAAA AATGGAGGCA GTCTGCTAAT TCAGGGACCA 
GACCACTGTT CACTCCTTCA CTACGCAGCT AAAACCGGCA ACGGGGAGAT TGTGAAATAT 
ATCCTTGACC ACGGACCTTC CGAGTTATTG GATATGGCAG ACAGTGAAAC GGGTGAGACT 
GCACTGCACA AGGCTGCCTG CCAGCGGAAC CGGGCTGTGT GCCAGCTTCT GGTGGATGCA 
GGAGCATCTC TGAGAAAGAC GGACTCCAAG GGTAAGACAC CTCAAGAAAG AGCACAGCAG 
GCTGGGGACC CAGACTTGGC TGCTTACCTA GAAAGCCGTC AGAACTATAA GGTCATTGGC 
CATGAGGACC TGGAAACTGC TGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012783.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868272.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu97591
Clone ID Related Accession (Same CDS sequence)	XM_017012783.1
Accession Version	XM_017012783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGGTGAAGAA ACCTCAGAAC 
TCCCTGAAGG CTTCAAATCG GAAGAAGAAG AGAACAAGCT TTAAAAGAAA AGCCAGTAAA 
AGAGGGATGG AACAGGAAAA CAAAGGTCGT CCTTTTGTGA TAAAACCCAT CTCTTCTCCT 
CTCATGAAAC CCTTGCTTGT ATTTGTGAAT CCCAAGAGTG GAGGCAACCA GGGAACCAAA 
GTCCTGCAGA TGTTCATGTG GTACCTGAAT CCACGGCAAG TCTTTGATCT TTCTCAGGAA 
GGGCCAAAAG ATGCGCTTGA ATTGTATAGG AAAGTACCAA ATCTGCGAAT TCTGGCCTGT 
GGTGGGGATG GAACGGTGGG CTGGATCCTT TCCATCCTGG ATGAACTGCA GCTGAGCCCT 
CAGCCTCCTG TGGGGGTCCT TCCTCTGGGG ACTGGGAATG ACCTGGCTCG AACTCTCAAC 
TGGGGAGGGG GCTACACTGA TGAACCTGTT TCTAAGATCC TGTGTCAAGT GGAAGATGGG 
ACAGTTGTAC AGCTAGATCG CTGGAACCTC CATGTGGAAA GAAACCCCGA CTTGCCTCCA 
GAAGAACTTG AAGATGGCGT ATGTAAGCTC CCTCTGAATG TTTTCAATAA CTACTTCAGC 
CTTGGATTTG ATGCCCATGT CACACTGGAG TTCCATGAAT CCAGAGAAGC AAATCCAGAG 
AAATTCAACA GTCGTTTTCG AAATAAAATG TTCTATGCAG GGGCAGCTTT TTCTGACTTC 
CTACAGAGAA GTTCTAGAGA TCTATCCAAA CATGTTAAAG TTGTTTGTGA TGGAACAGAT 
CTCACCCCAA AGATTCAGGA ACTGAAGTTC CAGTGTATAG TATTTTTAAA TATACCCAGA 
TATTGTGCTG GCACAATGCC CTGGGGAAAC CCAGGTGATC ACCATGATTT CGAACCTCAG 
CGTCATGATG ATGGTTATAT TGAAGTCATT GGATTTACCA TGGCCTCTTT GGCAGCCCTG 
CAAGTTGGGG GCCATGGAGA GAGGCTACAC CAGTGTCGAG AAGTCATGCT TCTAACTTAC 
AAATCCATCC CCATGCAAGT GGATGGGGAG CCCTGTAGGT TGGCCCCAGC TATGATTCGG 
ATCTCCCTGA GGAATCAGGC CAACATGGTA CAGAAGAGCA AGAGGAGAAC ATCCATGCCT 
TTACTCAATG ATATTCATCA GGTGCAAACT GCGGACCTGC GGCGAGTGTC TGCTCCCCCC 
GGCTCCTTCA CCATTCCCCA GTCTGTCCCA GATCGTCTGA GGATCCGGGT GAACAAAATC 
AGTTTACAAG ACTATGAAGG ATTCCACTAT GACAAGGAGA AACTCCGAGA AGCTTCGATA 
CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC TGTGATTTGG AGACTTGCCG TATGTACATA 
GACCGCCTAC AGGAGGACCT ACAGTCAGTT TCTTCTGGCT CCCAGAGAGT TCATTACCAG 
GACCATGAAA CCTCCTTCCC CAGGGCTCTC TCAGCACAGA GGCTCTCTCC TCGGTGGTGC 
TTCCTAGATG CAACTTCTGC TGATCGCTTT TATCGAATAG ACAGATCTCA GGAACATTTG 
CACTTTGTGA TGGAGATTTC CCAAGATGAG ATTTTTATTC TGGACCCAGA TATGGTGGTG 
TCACAGCCGG CGGGGACACC TCCGGGCATG CCTGACCTGG TGGTGGAACA AGCCTCGGGG 
TCACCAGTCT CTTCAGAAGA TCATGCAATT TTGCAGGCAG TAATAGCTGG TGATCTTATG 
AAGCTAATAG AAAGCTATAA AAATGGAGGC AGTCTGCTAA TTCAGGGACC AGACCACTGT 
TCACTCCTTC ACTACGCAGC TAAAACCGGC AACGGGGAGA TTGTGAAATA TATCCTTGAC 
CACGGACCTT CCGAGTTATT GGATATGGCA GACAGTGAAA CGGGTGAGAC TGCACTGCAC 
AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG TGCCAGCTTC TGGTGGATGC AGGAGCATCT 
CTGAGAAAGA CGGACTCCAA GGGTAAGACA CCTCAAGAAA GAGCACAGCA GGCTGGGGAC 
CCAGACTTGG CTGCTTACCT AGAAAGCCGT CAGAACTATA AGGTCATTGG CCATGAGGAC 
CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868272.1
CDS	156..3113
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012783.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGGTGAAGAA ACCTCAGAAC 
TCCCTGAAGG CTTCAAATCG GAAGAAGAAG AGAACAAGCT TTAAAAGAAA AGCCAGTAAA 
AGAGGGATGG AACAGGAAAA CAAAGGTCGT CCTTTTGTGA TAAAACCCAT CTCTTCTCCT 
CTCATGAAAC CCTTGCTTGT ATTTGTGAAT CCCAAGAGTG GAGGCAACCA GGGAACCAAA 
GTCCTGCAGA TGTTCATGTG GTACCTGAAT CCACGGCAAG TCTTTGATCT TTCTCAGGAA 
GGGCCAAAAG ATGCGCTTGA ATTGTATAGG AAAGTACCAA ATCTGCGAAT TCTGGCCTGT 
GGTGGGGATG GAACGGTGGG CTGGATCCTT TCCATCCTGG ATGAACTGCA GCTGAGCCCT 
CAGCCTCCTG TGGGGGTCCT TCCTCTGGGG ACTGGGAATG ACCTGGCTCG AACTCTCAAC 
TGGGGAGGGG GCTACACTGA TGAACCTGTT TCTAAGATCC TGTGTCAAGT GGAAGATGGG 
ACAGTTGTAC AGCTAGATCG CTGGAACCTC CATGTGGAAA GAAACCCCGA CTTGCCTCCA 
GAAGAACTTG AAGATGGCGT ATGTAAGCTC CCTCTGAATG TTTTCAATAA CTACTTCAGC 
CTTGGATTTG ATGCCCATGT CACACTGGAG TTCCATGAAT CCAGAGAAGC AAATCCAGAG 
AAATTCAACA GTCGTTTTCG AAATAAAATG TTCTATGCAG GGGCAGCTTT TTCTGACTTC 
CTACAGAGAA GTTCTAGAGA TCTATCCAAA CATGTTAAAG TTGTTTGTGA TGGAACAGAT 
CTCACCCCAA AGATTCAGGA ACTGAAGTTC CAGTGTATAG TATTTTTAAA TATACCCAGA 
TATTGTGCTG GCACAATGCC CTGGGGAAAC CCAGGTGATC ACCATGATTT CGAACCTCAG 
CGTCATGATG ATGGTTATAT TGAAGTCATT GGATTTACCA TGGCCTCTTT GGCAGCCCTG 
CAAGTTGGGG GCCATGGAGA GAGGCTACAC CAGTGTCGAG AAGTCATGCT TCTAACTTAC 
AAATCCATCC CCATGCAAGT GGATGGGGAG CCCTGTAGGT TGGCCCCAGC TATGATTCGG 
ATCTCCCTGA GGAATCAGGC CAACATGGTA CAGAAGAGCA AGAGGAGAAC ATCCATGCCT 
TTACTCAATG ATATTCATCA GGTGCAAACT GCGGACCTGC GGCGAGTGTC TGCTCCCCCC 
GGCTCCTTCA CCATTCCCCA GTCTGTCCCA GATCGTCTGA GGATCCGGGT GAACAAAATC 
AGTTTACAAG ACTATGAAGG ATTCCACTAT GACAAGGAGA AACTCCGAGA AGCTTCGATA 
CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC TGTGATTTGG AGACTTGCCG TATGTACATA 
GACCGCCTAC AGGAGGACCT ACAGTCAGTT TCTTCTGGCT CCCAGAGAGT TCATTACCAG 
GACCATGAAA CCTCCTTCCC CAGGGCTCTC TCAGCACAGA GGCTCTCTCC TCGGTGGTGC 
TTCCTAGATG CAACTTCTGC TGATCGCTTT TATCGAATAG ACAGATCTCA GGAACATTTG 
CACTTTGTGA TGGAGATTTC CCAAGATGAG ATTTTTATTC TGGACCCAGA TATGGTGGTG 
TCACAGCCGG CGGGGACACC TCCGGGCATG CCTGACCTGG TGGTGGAACA AGCCTCGGGG 
TCACCAGTCT CTTCAGAAGA TCATGCAATT TTGCAGGCAG TAATAGCTGG TGATCTTATG 
AAGCTAATAG AAAGCTATAA AAATGGAGGC AGTCTGCTAA TTCAGGGACC AGACCACTGT 
TCACTCCTTC ACTACGCAGC TAAAACCGGC AACGGGGAGA TTGTGAAATA TATCCTTGAC 
CACGGACCTT CCGAGTTATT GGATATGGCA GACAGTGAAA CGGGTGAGAC TGCACTGCAC 
AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG TGCCAGCTTC TGGTGGATGC AGGAGCATCT 
CTGAGAAAGA CGGACTCCAA GGGTAAGACA CCTCAAGAAA GAGCACAGCA GGCTGGGGAC 
CCAGACTTGG CTGCTTACCT AGAAAGCCGT CAGAACTATA AGGTCATTGG CCATGAGGAC 
CTGGAAACTG CTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_017012784.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016868273.1 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu97592
Clone ID Related Accession (Same CDS sequence)	XM_017012784.1
Accession Version	XM_017012784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGAA CTCCCTGAAG 
GCTTCAAATC GGAAGAAGAA GAGAACAAGC TTTAAAAGAA AAGCCAGTAA AAGAGGGATG 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCCA TCTCTTCTCC TCTCATGAAA 
CCCTTGCTTG TATTTGTGAA TCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTGCAG 
ATGTTCATGT GGTACCTGAA TCCACGGCAA GTCTTTGATC TTTCTCAGGA AGGGCCAAAA 
GATGCGCTTG AATTGTATAG GAAAGTACCA AATCTGCGAA TTCTGGCCTG TGGTGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATCCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTGGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GAACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAACCTGT TTCTAAGATC CTGTGTCAAG TGGAAGATGG GACAGTTGTA 
CAGCTAGATC GCTGGAACCT CCATGTGGAA AGAAACCCCG ACTTGCCTCC AGAAGAACTT 
GAAGATGGCG TATGTAAGCT CCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTTGGATTT 
GATGCCCATG TCACACTGGA GTTCCATGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGACTT CCTACAGAGA 
AGTTCTAGAG ATCTATCCAA ACATGTTAAA GTTGTTTGTG ATGGAACAGA TCTCACCCCA 
AAGATTCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGAAA CCCAGGTGAT CACCATGATT TCGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGATTTACC ATGGCCTCTT TGGCAGCCCT GCAAGTTGGG 
GGCCATGGAG AGAGGCTACA CCAGTGTCGA GAAGTCATGC TTCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGTAGG TTGGCCCCAG CTATGATTCG GATCTCCCTG 
AGGAATCAGG CCAACATGGT ACAGAAGAGC AAGAGGAGAA CATCCATGCC TTTACTCAAT 
GATATTCATC AGGTGCAAAC TGCGGACCTG CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC 
ACCATTCCCC AGTCTGTCCC AGATCGTCTG AGGATCCGGG TGAACAAAAT CAGTTTACAA 
GACTATGAAG GATTCCACTA TGACAAGGAG AAACTCCGAG AAGCTTCGAT ACCTCTGGGT 
ATTCTAGTTG TGCGTGGAGA CTGTGATTTG GAGACTTGCC GTATGTACAT AGACCGCCTA 
CAGGAGGACC TACAGTCAGT TTCTTCTGGC TCCCAGAGAG TTCATTACCA GGACCATGAA 
ACCTCCTTCC CCAGGGCTCT CTCAGCACAG AGGCTCTCTC CTCGGTGGTG CTTCCTAGAT 
GCAACTTCTG CTGATCGCTT TTATCGAATA GACAGATCTC AGGAACATTT GCACTTTGTG 
ATGGAGATTT CCCAAGATGA GATTTTTATT CTGGACCCAG ATATGGTGGT GTCACAGCCG 
GCGGGGACAC CTCCGGGCAT GCCTGACCTG GTGGTGGAAC AAGCCTCGGG GATCTCAGAC 
TGGTGGAATC CTGCCCTGCG GAAACGCATG CTGAGTGACA GTGGGCTGGG GATGATAGCT 
CCCTATTATG AGGACTCAGA TCTGAAAGAT CTCAGCCACT CCCGCGTGCT ACAGTCACCA 
GTCTCTTCAG AAGATCATGC AATTTTGCAG GCAGTAATAG CTGGTGATCT TATGAAGCTA 
ATAGAAAGCT ATAAAAATGG AGGCAGTCTG CTAATTCAGG GACCAGACCA CTGTTCACTC 
CTTCACTACG CAGCTAAAAC CGGCAACGGG GAGATTGTGA AATATATCCT TGACCACGGA 
CCTTCCGAGT TATTGGATAT GGCAGACAGT GAAACGGGTG AGACTGCACT GCACAAGGCT 
GCCTGCCAGC GGAACCGGGC TGTGTGCCAG CTTCTGGTGG ATGCAGGAGC ATCTCTGAGA 
AAGACGGACT CCAAGGGTAA GACACCTCAA GAAAGAGCAC AGCAGGCTGG GGACCCAGAC 
TTGGCTGCTT ACCTAGAAAG CCGTCAGAAC TATAAGGTCA TTGGCCATGA GGACCTGGAA 
ACTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016868273.1
CDS	156..3047
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV

Target ORF information:

RefSeq Version	XM_017012784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017012784.1

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGAA CTCCCTGAAG 
GCTTCAAATC GGAAGAAGAA GAGAACAAGC TTTAAAAGAA AAGCCAGTAA AAGAGGGATG 
GAACAGGAAA ACAAAGGTCG TCCTTTTGTG ATAAAACCCA TCTCTTCTCC TCTCATGAAA 
CCCTTGCTTG TATTTGTGAA TCCCAAGAGT GGAGGCAACC AGGGAACCAA AGTCCTGCAG 
ATGTTCATGT GGTACCTGAA TCCACGGCAA GTCTTTGATC TTTCTCAGGA AGGGCCAAAA 
GATGCGCTTG AATTGTATAG GAAAGTACCA AATCTGCGAA TTCTGGCCTG TGGTGGGGAT 
GGAACGGTGG GCTGGATCCT TTCCATCCTG GATGAACTGC AGCTGAGCCC TCAGCCTCCT 
GTGGGGGTCC TTCCTCTGGG GACTGGGAAT GACCTGGCTC GAACTCTCAA CTGGGGAGGG 
GGCTACACTG ATGAACCTGT TTCTAAGATC CTGTGTCAAG TGGAAGATGG GACAGTTGTA 
CAGCTAGATC GCTGGAACCT CCATGTGGAA AGAAACCCCG ACTTGCCTCC AGAAGAACTT 
GAAGATGGCG TATGTAAGCT CCCTCTGAAT GTTTTCAATA ACTACTTCAG CCTTGGATTT 
GATGCCCATG TCACACTGGA GTTCCATGAA TCCAGAGAAG CAAATCCAGA GAAATTCAAC 
AGTCGTTTTC GAAATAAAAT GTTCTATGCA GGGGCAGCTT TTTCTGACTT CCTACAGAGA 
AGTTCTAGAG ATCTATCCAA ACATGTTAAA GTTGTTTGTG ATGGAACAGA TCTCACCCCA 
AAGATTCAGG AACTGAAGTT CCAGTGTATA GTATTTTTAA ATATACCCAG ATATTGTGCT 
GGCACAATGC CCTGGGGAAA CCCAGGTGAT CACCATGATT TCGAACCTCA GCGTCATGAT 
GATGGTTATA TTGAAGTCAT TGGATTTACC ATGGCCTCTT TGGCAGCCCT GCAAGTTGGG 
GGCCATGGAG AGAGGCTACA CCAGTGTCGA GAAGTCATGC TTCTAACTTA CAAATCCATC 
CCCATGCAAG TGGATGGGGA GCCCTGTAGG TTGGCCCCAG CTATGATTCG GATCTCCCTG 
AGGAATCAGG CCAACATGGT ACAGAAGAGC AAGAGGAGAA CATCCATGCC TTTACTCAAT 
GATATTCATC AGGTGCAAAC TGCGGACCTG CGGCGAGTGT CTGCTCCCCC CGGCTCCTTC 
ACCATTCCCC AGTCTGTCCC AGATCGTCTG AGGATCCGGG TGAACAAAAT CAGTTTACAA 
GACTATGAAG GATTCCACTA TGACAAGGAG AAACTCCGAG AAGCTTCGAT ACCTCTGGGT 
ATTCTAGTTG TGCGTGGAGA CTGTGATTTG GAGACTTGCC GTATGTACAT AGACCGCCTA 
CAGGAGGACC TACAGTCAGT TTCTTCTGGC TCCCAGAGAG TTCATTACCA GGACCATGAA 
ACCTCCTTCC CCAGGGCTCT CTCAGCACAG AGGCTCTCTC CTCGGTGGTG CTTCCTAGAT 
GCAACTTCTG CTGATCGCTT TTATCGAATA GACAGATCTC AGGAACATTT GCACTTTGTG 
ATGGAGATTT CCCAAGATGA GATTTTTATT CTGGACCCAG ATATGGTGGT GTCACAGCCG 
GCGGGGACAC CTCCGGGCAT GCCTGACCTG GTGGTGGAAC AAGCCTCGGG GATCTCAGAC 
TGGTGGAATC CTGCCCTGCG GAAACGCATG CTGAGTGACA GTGGGCTGGG GATGATAGCT 
CCCTATTATG AGGACTCAGA TCTGAAAGAT CTCAGCCACT CCCGCGTGCT ACAGTCACCA 
GTCTCTTCAG AAGATCATGC AATTTTGCAG GCAGTAATAG CTGGTGATCT TATGAAGCTA 
ATAGAAAGCT ATAAAAATGG AGGCAGTCTG CTAATTCAGG GACCAGACCA CTGTTCACTC 
CTTCACTACG CAGCTAAAAC CGGCAACGGG GAGATTGTGA AATATATCCT TGACCACGGA 
CCTTCCGAGT TATTGGATAT GGCAGACAGT GAAACGGGTG AGACTGCACT GCACAAGGCT 
GCCTGCCAGC GGAACCGGGC TGTGTGCCAG CTTCTGGTGG ATGCAGGAGC ATCTCTGAGA 
AAGACGGACT CCAAGGGTAA GACACCTCAA GAAAGAGCAC AGCAGGCTGG GGACCCAGAC 
TTGGCTGCTT ACCTAGAAAG CCGTCAGAAC TATAAGGTCA TTGGCCATGA GGACCTGGAA 
ACTGCTGTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKI ( XM_011516682.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011514984.2 Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X6, mRNA.

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CloneID	OHu97593
Clone ID Related Accession (Same CDS sequence)	XM_011516682.2
Accession Version	XM_011516682.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	diacylglycerol kinase iota isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011516682.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGGTGAAGAA ACCTCAGAAC 
TCCCTGAAGG CTTCAAATCG GAAGAAGAAG AGAACAAGCT TTAAAAGAAA AGCCAGTAAA 
AGAGGGATGG AACAGGAAAA CAAAGGTCGT CCTTTTGTGA TAAAACCCAT CTCTTCTCCT 
CTCATGAAAC CCTTGCTTGT ATTTGTGAAT CCCAAGAGTG GAGGCAACCA GGGAACCAAA 
GTCCTGCAGA TGTTCATGTG GTACCTGAAT CCACGGCAAG TCTTTGATCT TTCTCAGGAA 
GGGCCAAAAG ATGCGCTTGA ATTGTATAGG AAAGTACCAA ATCTGCGAAT TCTGGCCTGT 
GGTGGGGATG GAACGGTGGG CTGGATCCTT TCCATCCTGG ATGAACTGCA GCTGAGCCCT 
CAGCCTCCTG TGGGGGTCCT TCCTCTGGGG ACTGGGAATG ACCTGGCTCG AACTCTCAAC 
TGGGGAGGGG GCTACACTGA TGAACCTGTT TCTAAGATCC TGTGTCAAGT GGAAGATGGG 
ACAGTTGTAC AGCTAGATCG CTGGAACCTC CATGTGGAAA GAAACCCCGA CTTGCCTCCA 
GAAGAACTTG AAGATGGCGT ATGTAAGCTC CCTCTGAATG TTTTCAATAA CTACTTCAGC 
CTTGGATTTG ATGCCCATGT CACACTGGAG TTCCATGAAT CCAGAGAAGC AAATCCAGAG 
AAATTCAACA GTCGTTTTCG AAATAAAATG TTCTATGCAG GGGCAGCTTT TTCTGACTTC 
CTACAGAGAA GTTCTAGAGA TCTATCCAAA CATGTTAAAG TTGTTTGTGA TGGAACAGAT 
CTCACCCCAA AGATTCAGGA ACTGAAGTTC CAGTGTATAG TATTTTTAAA TATACCCAGA 
TATTGTGCTG GCACAATGCC CTGGGGAAAC CCAGGTGATC ACCATGATTT CGAACCTCAG 
CGTCATGATG ATGGTTATAT TGAAGTCATT GGATTTACCA TGGCCTCTTT GGCAGCCCTG 
CAAGTTGGGG GCCATGGAGA GAGGCTACAC CAGTGTCGAG AAGTCATGCT TCTAACTTAC 
AAATCCATCC CCATGCAAGT GGATGGGGAG CCCTGTAGGT TGGCCCCAGC TATGATTCGG 
ATCTCCCTGA GGAATCAGGC CAACATGGTA CAGAAGAGCA AGAGGAGAAC ATCCATGCCT 
TTACTCAATG ATATTCATCA GGTGCAAACT GCGGACCTGC GGCGAGTGTC TGCTCCCCCC 
GGCTCCTTCA CCATTCCCCA GTCTGTCCCA GATCGTCTGA GGATCCGGGT GAACAAAATC 
AGTTTACAAG ACTATGAAGG ATTCCACTAT GACAAGGAGA AACTCCGAGA AGCTTCGATA 
CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC TGTGATTTGG AGACTTGCCG TATGTACATA 
GACCGCCTAC AGGAGGACCT ACAGTCAGTT TCTTCTGGCT CCCAGAGAGT TCATTACCAG 
GACCATGAAA CCTCCTTCCC CAGGGCTCTC TCAGCACAGA GGCTCTCTCC TCGGTGGTGC 
TTCCTAGATG CAACTTCTGC TGATCGCTTT TATCGAATAG ACAGATCTCA GGAACATTTG 
CACTTTGTGA TGGAGATTTC CCAAGATGAG ATTTTTATTC TGGACCCAGA TATGGTGGTG 
TCACAGCCGG CGGGGACACC TCCGGGCATG CCTGACCTGG TGGTGGAACA AGCCTCGGGG 
ATCTCAGACT GGTGGAATCC TGCCCTGCGG AAACGCATGC TGAGTGACAG TGGGCTGGGG 
ATGATAGCTC CCTATTATGA GGACTCAGAT CTGAAAGATC TCAGCCACTC CCGCGTGCTA 
CAGTCACCAG TCTCTTCAGA AGATCATGCA ATTTTGCAGG CAGTAATAGC TGGTGATCTT 
ATGAAGCTAA TAGAAAGCTA TAAAAATGGA GGCAGTCTGC TAATTCAGGG ACCAGACCAC 
TGTTCACTCC TTCACTACGC AGCTAAAACC GGCAACGGGG AGATTGTGAA ATATATCCTT 
GACCACGTCT CTTGCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011514984.2
CDS	156..2996
Translation	MNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDIHQVQTADLRRVSAPPGSFTIPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHVSCS

Target ORF information:

RefSeq Version	XM_011516682.2
Organism	Homo sapiens(human)
Definition	Homo sapiens diacylglycerol kinase iota (DGKI), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011516682.2

ORF Insert Sequence:

ATGAACCCCA GCTCCTCGGC GGGAGAGGAG AAAGGGGCGA CGGGCGGCAG CAGCAGCAGC 
GGAAGCGGCG CCGGGAGCTG CTGCCTGGGC GCCGAGGGCG GCGCGGACCC GCGGGGCGCA 
GGGTCAGCCG CGGCGGCGGG GGCCGCTGCC CTGGACGAGC CCGCGGCCGC CGGCCAGAAG 
GAGAAGGACG AAGCGCTGGA GGAGAAGCTG AGGAACTTAA CTTTCCGGAA GCAGGTCTCG 
TACAGGAAAG CAATCTCCCG GGCAGGCCTC CAGCATCTGG CTCCTGCACA TCCCCTCAGC 
CTTCCTGTGG CAAATGGTCC AGCCAAGGAG CCCAGAGCGA CTTTGGACTG GAGTGAGAAT 
GCCGTGAATG GAGAACACCT GTGGCTGGAG ACCAACGTCT CGGGAGACCT CTGCTACCTT 
GGAGAGGAGA ACTGCCAAGT CAGATTTGCA AAATCAGCTC TCAGGAGGAA GTGTGCAGTC 
TGTAAAATCG TCGTCCACAC CGCCTGCATT GAGCAGCTAG AAAAGATTAA TTTCAGATGT 
AAACCAACAT TTCGAGAAGG AGGCTCAAGG TCACCAAGAG AAAATTTTGT ACGTCATCAC 
TGGGTGCACA GGCGTCGGCA GGAGGGGAAA TGTAAGCAGT GTGGTAAGGG CTTCCAGCAA 
AAGTTCTCCT TCCACAGTAA AGAGATTGTG GCTATCAGCT GTTCCTGGTG CAAGCAGGCG 
TTTCACAATA AGGTGACCTG CTTCATGCTG CATCACATTG AAGAACCCTG CTCCCTGGGG 
GCTCATGCTG CTGTTATTGT CCCGCCCACT TGGATCATTA AGGTGAAGAA ACCTCAGAAC 
TCCCTGAAGG CTTCAAATCG GAAGAAGAAG AGAACAAGCT TTAAAAGAAA AGCCAGTAAA 
AGAGGGATGG AACAGGAAAA CAAAGGTCGT CCTTTTGTGA TAAAACCCAT CTCTTCTCCT 
CTCATGAAAC CCTTGCTTGT ATTTGTGAAT CCCAAGAGTG GAGGCAACCA GGGAACCAAA 
GTCCTGCAGA TGTTCATGTG GTACCTGAAT CCACGGCAAG TCTTTGATCT TTCTCAGGAA 
GGGCCAAAAG ATGCGCTTGA ATTGTATAGG AAAGTACCAA ATCTGCGAAT TCTGGCCTGT 
GGTGGGGATG GAACGGTGGG CTGGATCCTT TCCATCCTGG ATGAACTGCA GCTGAGCCCT 
CAGCCTCCTG TGGGGGTCCT TCCTCTGGGG ACTGGGAATG ACCTGGCTCG AACTCTCAAC 
TGGGGAGGGG GCTACACTGA TGAACCTGTT TCTAAGATCC TGTGTCAAGT GGAAGATGGG 
ACAGTTGTAC AGCTAGATCG CTGGAACCTC CATGTGGAAA GAAACCCCGA CTTGCCTCCA 
GAAGAACTTG AAGATGGCGT ATGTAAGCTC CCTCTGAATG TTTTCAATAA CTACTTCAGC 
CTTGGATTTG ATGCCCATGT CACACTGGAG TTCCATGAAT CCAGAGAAGC AAATCCAGAG 
AAATTCAACA GTCGTTTTCG AAATAAAATG TTCTATGCAG GGGCAGCTTT TTCTGACTTC 
CTACAGAGAA GTTCTAGAGA TCTATCCAAA CATGTTAAAG TTGTTTGTGA TGGAACAGAT 
CTCACCCCAA AGATTCAGGA ACTGAAGTTC CAGTGTATAG TATTTTTAAA TATACCCAGA 
TATTGTGCTG GCACAATGCC CTGGGGAAAC CCAGGTGATC ACCATGATTT CGAACCTCAG 
CGTCATGATG ATGGTTATAT TGAAGTCATT GGATTTACCA TGGCCTCTTT GGCAGCCCTG 
CAAGTTGGGG GCCATGGAGA GAGGCTACAC CAGTGTCGAG AAGTCATGCT TCTAACTTAC 
AAATCCATCC CCATGCAAGT GGATGGGGAG CCCTGTAGGT TGGCCCCAGC TATGATTCGG 
ATCTCCCTGA GGAATCAGGC CAACATGGTA CAGAAGAGCA AGAGGAGAAC ATCCATGCCT 
TTACTCAATG ATATTCATCA GGTGCAAACT GCGGACCTGC GGCGAGTGTC TGCTCCCCCC 
GGCTCCTTCA CCATTCCCCA GTCTGTCCCA GATCGTCTGA GGATCCGGGT GAACAAAATC 
AGTTTACAAG ACTATGAAGG ATTCCACTAT GACAAGGAGA AACTCCGAGA AGCTTCGATA 
CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC TGTGATTTGG AGACTTGCCG TATGTACATA 
GACCGCCTAC AGGAGGACCT ACAGTCAGTT TCTTCTGGCT CCCAGAGAGT TCATTACCAG 
GACCATGAAA CCTCCTTCCC CAGGGCTCTC TCAGCACAGA GGCTCTCTCC TCGGTGGTGC 
TTCCTAGATG CAACTTCTGC TGATCGCTTT TATCGAATAG ACAGATCTCA GGAACATTTG 
CACTTTGTGA TGGAGATTTC CCAAGATGAG ATTTTTATTC TGGACCCAGA TATGGTGGTG 
TCACAGCCGG CGGGGACACC TCCGGGCATG CCTGACCTGG TGGTGGAACA AGCCTCGGGG 
ATCTCAGACT GGTGGAATCC TGCCCTGCGG AAACGCATGC TGAGTGACAG TGGGCTGGGG 
ATGATAGCTC CCTATTATGA GGACTCAGAT CTGAAAGATC TCAGCCACTC CCGCGTGCTA 
CAGTCACCAG TCTCTTCAGA AGATCATGCA ATTTTGCAGG CAGTAATAGC TGGTGATCTT 
ATGAAGCTAA TAGAAAGCTA TAAAAATGGA GGCAGTCTGC TAATTCAGGG ACCAGACCAC 
TGTTCACTCC TTCACTACGC AGCTAAAACC GGCAACGGGG AGATTGTGAA ATATATCCTT 
GACCACGTCT CTTGCAGTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DGKI
Entrez Gene ID	9162
Full Name	diacylglycerol kinase iota
Synonyms	DGK-IOTA
General protein information	Preferred Names diacylglycerol kinase iota Names diacylglycerol kinase iota DAG kinase iota diglyceride kinase iota
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q33
Summary	This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008].
Disorder MIM:	604072

	DGKI	XP_001107030.1
Canis lupus familiaris (dog)	DGKI	XP_005629798.1
Bos taurus (cattle)	DGKI	XP_002687107.1
Rattus norvegicus (Norway rat)	Dgki	NP_942077.2
Danio rerio (zebrafish)	dgki	XP_002667022.3
Xenopus tropicalis (tropical clawed frog)	dgki	XP_002942047.2
Gallus gallus (chicken)	DGKI	XP_416357.4
Homo sapiens (human)	DGKI	NP_004708.1
Pan troglodytes (chimpanzee)	DGKI	XP_001147695.1
Mus musculus (house mouse)	Dgki	NP_001074675.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DGKI cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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