ROBO1 cDNA ORF clone, Gallus gallus(chicken)

The following ROBO1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa142916 XM_025144724.1
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa142917 XM_025144725.1
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OGa30202 XM_015298481.2
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30203 XM_015298487.2
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30204 XM_015298492.2
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30205 XM_416673.6
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30207 XM_015298516.2
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa02612 XM_004938345.3
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa30206 XM_015298510.2
Latest version!
Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
View Old Accession Versions >>
OGa30205 XM_416673.5 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30202 XM_015298481.1 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30207 XM_015298516.1 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa02612 XM_004938345.2 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa30206 XM_015298510.1 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OGa30203 XM_015298487.1 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OGa30204 XM_015298492.1 Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa142916
    Clone ID Related Accession (Same CDS sequence) XM_025144724.1
    Accession Version XM_025144724.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4647bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGTTCTACGG
    GATGACTTCA GACAAAATCC ATCAGATGTA ATGGTAGCTG TGGGTGAACC TGCTGTGATG
    GAATGTCAGC CCCCAAGAGG TCACCCAGAA CCCACTATAT CATGGAAAAA AGATGGGACT
    CCTCTAGATG ACAAAGATGA GAGAATTACA ATCAGAGGTG GAAAGCTTAT GATCACATAC
    ACAAGAAAGA ATGATGCTGG CAAGTACGTT TGTGTTGGAA CAAATATGGT TGGAGAACGT
    GAAAGTGAAG TTGCAGAGCT CACTGTTTTA GAAAGACCGT CTTTTGTGAA GAGACCAAGT
    AATTTGGCTG TAACTGTGGA TGACAGTGCT GAGTTTAAAT GTGAGGCAAG AGGTGACCCA
    GTCCCTACAG TAAGATGGAG GAAAGATGAT GGGGAATTAC CAAAAGCGAG ATATGAAATT
    CGTGATGATC ATACCCTGAA AATCCGGAAA GTTATGGCAG GAGACATGGG CTCATATACT
    TGTGTAGCAG AAAATATGGT TGGAAAAGCT GAAGCATCGG CAACTCTAAC AGTGCAAGTT
    GTGTCTGAGC CTCCACAGTT TGTTGTAAAG CCTCGTGATC AGGTAGCTGC ATTGGGAAGA
    ACAGTGACTT TCCAGTGTGA AGCAACTGGG AACCCACAGC CAGCCATCTT TTGGCGGAGA
    GAGGGAAGTC AGAATCTGCT TTTCTCATAC CAGCCCCCCC AGTCATCCAG TCGATTTTCA
    GTTTCCCAGA CAGGAGACCT CACCATCACC AATGTCCAGA GATCTGATGT TGGGTACTAC
    ATCTGCCAGA CTTTAAATGT TGCAGGAAGC ATCATAACAA AAGCCTACTT GGAAGTTACT
    GATGTGATTG CAGACCGGCC TCCCCCTGTC ATTCGACAGG GTCCTGTGAA TCAGACTGTA
    GCTGTAGATG GTACTTTAGT ACTAAACTGT GTGGCCACAG GCACTTTAAC ACCCACTATC
    CTCTGGAAAA AGGATGGGAC CCTGATCTCT ACCCAGGACT CTCGCATCAA ACAGTTGGAG
    ACAGGAGCAT TACAGATCCG ATATGCCAAG CTGGGTGATA CTGGTCGGTA CACCTGCGTT
    GCTTCAACCC CAAGTGGCGA AGCTACATGG AGTGCCTACA TAGAGGTCCA AGAATTTGGA
    GTCCCAGTGC AGCCACCAAG ACCCACTGAC CCAAACTTGA TTCCTAGTGC TCCATCAAAA
    CCTGAGGTTA CAGATGTCAG CAGAAATACA GTAACATTGT CATGGCAACC TAATTTGAAT
    TCAGGTGCAA CGCCAACCTC TTACATAATT GAGGCCTTCA GCCATGCATC TGGTAGCAGC
    TGGCAAACTG TAGCTGAAAA TGTGAAAACA GAGAGTTTTG CAGTTAAAGG GCTAAAACCT
    AGTGCAATTT ATCTATTCCT TGTGAGGGCA GCTAATGCAT ATGGACTAAG TGACCCAAGC
    CAAATATCTG ATCCAGTGAA AACTCAAGAT GTTCCACCAA CAAGTCAAGG TGTGGATCAC
    AAGCAGGTGC AGAGAGAACT AGGCGATGTG GTACTACATC TGCACAACCC TACTATCCTT
    TCTTCCTCCT CAATTGAAGT TCATTGGACA GTGGACCAGC AATCCCAATA TATTCAAGGA
    TATAAAATCC TATACCGGCC AACACCAGCG TCCTATGGAG AGTCAGAATG GTTAATTTTT
    GAAGTGAGGA CTCCAACCAA AAACAGTGTT ATCATTCCAG AGCTGAAGAA AGGTGTAAAC
    TATGAAATCA AGGCCCGCCC TTTTTTTAAT GAGTTTCAAG GCGCAGATAG TGAAGTGAAA
    TTTGCAAAAA CCCTAGAAGA AGCGCCCAGT GCTCCACCTC AGAGTGTTTC AGTAACTAAA
    AATGATGGGA ATGGAACTGC AATTGTAGTC ACCTGGCAGC CACCCCCCGA GGATAACCAG
    AACGGAATGG TTCAAGAATA CAAGGTTTGG TGCCTTGGCA ATGAAAGTCG ATACCATATA
    AACAGGACAG TAGATGGTTC CACCTTTTCA GTAGTCATCC CTTCCCTAGT ACCAGGTATT
    CGATACAGCG TGGAAGTGGC AGCAAGTACT GGAGCAGGAC CTGGAGTGAA AAGTGATCCC
    CAGTTTATCC AGTTAGATTC GCATGGAAAT CCTGTGTCTT CAGAAGATCA AGTCAGTTTA
    GCCCAGCAGA TCTCTGACGT AGTAAAACAG CCTGCCTTTA TTGCTGGAAT TGGTGCTGCT
    TGTTGGATTA TCCTCATGGT CTTCAGCATC TGGTTGTATC GCCACAGAAA GAAGCGAAAT
    GGACTCACTA GTACTTACGC TGGTATCAGA AAAGTCCCGT CTTTTACCTT CACACCAACA
    GTAACCTATC AGAGAGGAGG AGATGCCGTA AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC
    ATCAGTGAAC CAGCCACACA GCCCTGGCTA GCAGATACTT GGCCTAACAC AGGGAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACC TCTGGCAATG GAAACAGTGA TGGCAACCTT
    ACGACATACA GTCGACCAGC CGATTGTATA GCAAATTACA ACAATCAGCT GGACAACAAG
    CAGACAAACC TGATGTTGCC TGAGTCAACT GTATATGGTG ACGTGGACCT AAGCAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGTCCA AATCTAAAGG ATGGAAGATT TGTCAGCCAA
    CCTGGGCAGC CCACTCCTTA TGCCACCACT CAACTCATCC AGTCAAATAT CAGCAATAAT
    GTTAACAATG GCAGCGGGGA TACGAATGAG AAACACTGGA AACCCTCTGT TCAGCAAAAG
    CAAGAGGTTG CACCCATACA GTACAACATT ATGGAGCAAA ACAAATTAAA CAAAGATTAC
    CGAGTAAATG ACAGCATTGC TCCAACAATT CCATACAACC AGTCATATGA CCAGAATACA
    GGTGGATCCT ACAACAGCTC AGATAGAGGC AGTAGCACAT CTGGGAGTCA AGGGCACAAG
    AAGGGTGCTC GGACTCCAAA GGCTCCCAAG CAAGCTGGCA TGAACTGGGC AGATCTTCTT
    CCACCCCCTC CAGCACATCC ACCTCCCCAT AGCAATAGTG AAGATTACAG TCTTTCAGTG
    GATGAAAGCT ATGACCAAGA AATTCCATGT CCAGTCCCAC CTGCAAGGAT GTATCTGCAG
    CAAGATGAGC TAGAGGAAGA GGAAGAAGAT GAGCGAGGTC CTACTCCACC TGTCCGAGGA
    GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT AGTCATCAGT CAACTGCCAC TCTCACCCCT
    TCTCCCCAGG AAGAACTCCA GCCCATGTTA CAGGACTGTC AGGAGGATCT GGGACACATA
    CAACACCAAC CTGAACGGAG ACGTCAGCCT GTGAGCCCTC CACCCCCTCC AAGGCCTATC
    TCTCCACCAC ATACCTATGG ATATATCTCA GGGCCTTTAG TCTCTGACAT GGATACTGAT
    GCACCAGAAG AGGAAGAAGA TGAGGCAGAT ATGGAGGTTG CTAAGATGCA AAACAGAAGA
    CTCCTTTTGC GTGGCCTTGA GCAGACACCT GCTTCAAGTG TTGGTGATTT GGAGAGCTCT
    GTAACAGGGT CCATGATCAA TGGCTGGGGT TCAGCTTCCG AAGAAGATAA CATCTCAAGT
    GGGCGGTCTA GTGTTAGCTC TTCAGATGGA TCATTTTTTA CTGATGCAGA TTTTGCACAG
    GCTGTTGCAG CAGCTGCAGA ATATGCTGGC TTGAAAGTAG CCAGACGTCA AATGCAGGAT
    GCAGCAGGAG GCCGTAGACA TTTTCATGCA TCACATTGTC CCAGACCTAC CAGCCCTGTA
    TCTACTGACA GCAACATGAG TGCTGTTGTA ATACAGAAGG TCCGACCTGC CAAAAAGCAA
    AAACACCAGC CAGGACATCT GCGCAGAGAA GTCTACACAG ATGACTTGCC ACCCCCTCCC
    GTGCCACCTC CCGCTATAAA ATCACCAACC GCCCAGTCCA AATCTCAGCT GGAGGTGCGA
    CCTGTCATGT TGCCAAAACT GGCTTCTATA GAACCGAGGA CAGACAGATC TGCGGAAAGA
    AAAGGAGTGA GCTACAAGGG GAGGGACGGG GTGGATGGGA GACAGCATTC AGACATGCGG
    ACAAATTCAG GAGAGCGGAG AGAATCACAG GAACAGCAAG GCGACGGGAA ACTGCGAGGA
    AGTAAAGCAC CAAAACGAGA AGGCACACCA GCAAAAACTC ATCTTCTTCA AGAGGACATC
    CTGCCATATT CTAGACCAAC GTTTCCAACG TCAAATAATC CCAGAGATCC CAGCTCCTCC
    AGCTCTATGT CTTCGAGAGG ATCAGGAGGG AGACAGAGGG CAGATCAAGC AAATATAGCC
    CGAAGAAATG TTGCAGAAAT GCAAGTACTG GGAGCATATG AACAAGGAGA AGAAGAAGAA
    GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000492.1
    CDS322..4968
    Translation

    Target ORF information:

    RefSeq Version XM_025144724.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144724.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGTTCTACGG
    GATGACTTCA GACAAAATCC ATCAGATGTA ATGGTAGCTG TGGGTGAACC TGCTGTGATG
    GAATGTCAGC CCCCAAGAGG TCACCCAGAA CCCACTATAT CATGGAAAAA AGATGGGACT
    CCTCTAGATG ACAAAGATGA GAGAATTACA ATCAGAGGTG GAAAGCTTAT GATCACATAC
    ACAAGAAAGA ATGATGCTGG CAAGTACGTT TGTGTTGGAA CAAATATGGT TGGAGAACGT
    GAAAGTGAAG TTGCAGAGCT CACTGTTTTA GAAAGACCGT CTTTTGTGAA GAGACCAAGT
    AATTTGGCTG TAACTGTGGA TGACAGTGCT GAGTTTAAAT GTGAGGCAAG AGGTGACCCA
    GTCCCTACAG TAAGATGGAG GAAAGATGAT GGGGAATTAC CAAAAGCGAG ATATGAAATT
    CGTGATGATC ATACCCTGAA AATCCGGAAA GTTATGGCAG GAGACATGGG CTCATATACT
    TGTGTAGCAG AAAATATGGT TGGAAAAGCT GAAGCATCGG CAACTCTAAC AGTGCAAGTT
    GTGTCTGAGC CTCCACAGTT TGTTGTAAAG CCTCGTGATC AGGTAGCTGC ATTGGGAAGA
    ACAGTGACTT TCCAGTGTGA AGCAACTGGG AACCCACAGC CAGCCATCTT TTGGCGGAGA
    GAGGGAAGTC AGAATCTGCT TTTCTCATAC CAGCCCCCCC AGTCATCCAG TCGATTTTCA
    GTTTCCCAGA CAGGAGACCT CACCATCACC AATGTCCAGA GATCTGATGT TGGGTACTAC
    ATCTGCCAGA CTTTAAATGT TGCAGGAAGC ATCATAACAA AAGCCTACTT GGAAGTTACT
    GATGTGATTG CAGACCGGCC TCCCCCTGTC ATTCGACAGG GTCCTGTGAA TCAGACTGTA
    GCTGTAGATG GTACTTTAGT ACTAAACTGT GTGGCCACAG GCACTTTAAC ACCCACTATC
    CTCTGGAAAA AGGATGGGAC CCTGATCTCT ACCCAGGACT CTCGCATCAA ACAGTTGGAG
    ACAGGAGCAT TACAGATCCG ATATGCCAAG CTGGGTGATA CTGGTCGGTA CACCTGCGTT
    GCTTCAACCC CAAGTGGCGA AGCTACATGG AGTGCCTACA TAGAGGTCCA AGAATTTGGA
    GTCCCAGTGC AGCCACCAAG ACCCACTGAC CCAAACTTGA TTCCTAGTGC TCCATCAAAA
    CCTGAGGTTA CAGATGTCAG CAGAAATACA GTAACATTGT CATGGCAACC TAATTTGAAT
    TCAGGTGCAA CGCCAACCTC TTACATAATT GAGGCCTTCA GCCATGCATC TGGTAGCAGC
    TGGCAAACTG TAGCTGAAAA TGTGAAAACA GAGAGTTTTG CAGTTAAAGG GCTAAAACCT
    AGTGCAATTT ATCTATTCCT TGTGAGGGCA GCTAATGCAT ATGGACTAAG TGACCCAAGC
    CAAATATCTG ATCCAGTGAA AACTCAAGAT GTTCCACCAA CAAGTCAAGG TGTGGATCAC
    AAGCAGGTGC AGAGAGAACT AGGCGATGTG GTACTACATC TGCACAACCC TACTATCCTT
    TCTTCCTCCT CAATTGAAGT TCATTGGACA GTGGACCAGC AATCCCAATA TATTCAAGGA
    TATAAAATCC TATACCGGCC AACACCAGCG TCCTATGGAG AGTCAGAATG GTTAATTTTT
    GAAGTGAGGA CTCCAACCAA AAACAGTGTT ATCATTCCAG AGCTGAAGAA AGGTGTAAAC
    TATGAAATCA AGGCCCGCCC TTTTTTTAAT GAGTTTCAAG GCGCAGATAG TGAAGTGAAA
    TTTGCAAAAA CCCTAGAAGA AGCGCCCAGT GCTCCACCTC AGAGTGTTTC AGTAACTAAA
    AATGATGGGA ATGGAACTGC AATTGTAGTC ACCTGGCAGC CACCCCCCGA GGATAACCAG
    AACGGAATGG TTCAAGAATA CAAGGTTTGG TGCCTTGGCA ATGAAAGTCG ATACCATATA
    AACAGGACAG TAGATGGTTC CACCTTTTCA GTAGTCATCC CTTCCCTAGT ACCAGGTATT
    CGATACAGCG TGGAAGTGGC AGCAAGTACT GGAGCAGGAC CTGGAGTGAA AAGTGATCCC
    CAGTTTATCC AGTTAGATTC GCATGGAAAT CCTGTGTCTT CAGAAGATCA AGTCAGTTTA
    GCCCAGCAGA TCTCTGACGT AGTAAAACAG CCTGCCTTTA TTGCTGGAAT TGGTGCTGCT
    TGTTGGATTA TCCTCATGGT CTTCAGCATC TGGTTGTATC GCCACAGAAA GAAGCGAAAT
    GGACTCACTA GTACTTACGC TGGTATCAGA AAAGTCCCGT CTTTTACCTT CACACCAACA
    GTAACCTATC AGAGAGGAGG AGATGCCGTA AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC
    ATCAGTGAAC CAGCCACACA GCCCTGGCTA GCAGATACTT GGCCTAACAC AGGGAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACC TCTGGCAATG GAAACAGTGA TGGCAACCTT
    ACGACATACA GTCGACCAGC CGATTGTATA GCAAATTACA ACAATCAGCT GGACAACAAG
    CAGACAAACC TGATGTTGCC TGAGTCAACT GTATATGGTG ACGTGGACCT AAGCAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGTCCA AATCTAAAGG ATGGAAGATT TGTCAGCCAA
    CCTGGGCAGC CCACTCCTTA TGCCACCACT CAACTCATCC AGTCAAATAT CAGCAATAAT
    GTTAACAATG GCAGCGGGGA TACGAATGAG AAACACTGGA AACCCTCTGT TCAGCAAAAG
    CAAGAGGTTG CACCCATACA GTACAACATT ATGGAGCAAA ACAAATTAAA CAAAGATTAC
    CGAGTAAATG ACAGCATTGC TCCAACAATT CCATACAACC AGTCATATGA CCAGAATACA
    GGTGGATCCT ACAACAGCTC AGATAGAGGC AGTAGCACAT CTGGGAGTCA AGGGCACAAG
    AAGGGTGCTC GGACTCCAAA GGCTCCCAAG CAAGCTGGCA TGAACTGGGC AGATCTTCTT
    CCACCCCCTC CAGCACATCC ACCTCCCCAT AGCAATAGTG AAGATTACAG TCTTTCAGTG
    GATGAAAGCT ATGACCAAGA AATTCCATGT CCAGTCCCAC CTGCAAGGAT GTATCTGCAG
    CAAGATGAGC TAGAGGAAGA GGAAGAAGAT GAGCGAGGTC CTACTCCACC TGTCCGAGGA
    GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT AGTCATCAGT CAACTGCCAC TCTCACCCCT
    TCTCCCCAGG AAGAACTCCA GCCCATGTTA CAGGACTGTC AGGAGGATCT GGGACACATA
    CAACACCAAC CTGAACGGAG ACGTCAGCCT GTGAGCCCTC CACCCCCTCC AAGGCCTATC
    TCTCCACCAC ATACCTATGG ATATATCTCA GGGCCTTTAG TCTCTGACAT GGATACTGAT
    GCACCAGAAG AGGAAGAAGA TGAGGCAGAT ATGGAGGTTG CTAAGATGCA AAACAGAAGA
    CTCCTTTTGC GTGGCCTTGA GCAGACACCT GCTTCAAGTG TTGGTGATTT GGAGAGCTCT
    GTAACAGGGT CCATGATCAA TGGCTGGGGT TCAGCTTCCG AAGAAGATAA CATCTCAAGT
    GGGCGGTCTA GTGTTAGCTC TTCAGATGGA TCATTTTTTA CTGATGCAGA TTTTGCACAG
    GCTGTTGCAG CAGCTGCAGA ATATGCTGGC TTGAAAGTAG CCAGACGTCA AATGCAGGAT
    GCAGCAGGAG GCCGTAGACA TTTTCATGCA TCACATTGTC CCAGACCTAC CAGCCCTGTA
    TCTACTGACA GCAACATGAG TGCTGTTGTA ATACAGAAGG TCCGACCTGC CAAAAAGCAA
    AAACACCAGC CAGGACATCT GCGCAGAGAA GTCTACACAG ATGACTTGCC ACCCCCTCCC
    GTGCCACCTC CCGCTATAAA ATCACCAACC GCCCAGTCCA AATCTCAGCT GGAGGTGCGA
    CCTGTCATGT TGCCAAAACT GGCTTCTATA GAACCGAGGA CAGACAGATC TGCGGAAAGA
    AAAGGAGTGA GCTACAAGGG GAGGGACGGG GTGGATGGGA GACAGCATTC AGACATGCGG
    ACAAATTCAG GAGAGCGGAG AGAATCACAG GAACAGCAAG GCGACGGGAA ACTGCGAGGA
    AGTAAAGCAC CAAAACGAGA AGGCACACCA GCAAAAACTC ATCTTCTTCA AGAGGACATC
    CTGCCATATT CTAGACCAAC GTTTCCAACG TCAAATAATC CCAGAGATCC CAGCTCCTCC
    AGCTCTATGT CTTCGAGAGG ATCAGGAGGG AGACAGAGGG CAGATCAAGC AAATATAGCC
    CGAAGAAATG TTGCAGAAAT GCAAGTACTG GGAGCATATG AACAAGGAGA AGAAGAAGAA
    GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa142917
    Clone ID Related Accession (Same CDS sequence) XM_025144725.1
    Accession Version XM_025144725.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTTGCGG AGTTTGCTCA CTTTTACCTC TGTGGATTAC TCTGCCTCGC CTCAGTTCTA 
    CGGGATGACT TCAGACAAAA TCCATCAGAT GTAATGGTAG CTGTGGGTGA ACCTGCTGTG
    ATGGAATGTC AGCCCCCAAG AGGTCACCCA GAACCCACTA TATCATGGAA AAAAGATGGG
    ACTCCTCTAG ATGACAAAGA TGAGAGAATT ACAATCAGAG GTGGAAAGCT TATGATCACA
    TACACAAGAA AGAATGATGC TGGCAAGTAC GTTTGTGTTG GAACAAATAT GGTTGGAGAA
    CGTGAAAGTG AAGTTGCAGA GCTCACTGTT TTAGAAAGAC CGTCTTTTGT GAAGAGACCA
    AGTAATTTGG CTGTAACTGT GGATGACAGT GCTGAGTTTA AATGTGAGGC AAGAGGTGAC
    CCAGTCCCTA CAGTAAGATG GAGGAAAGAT GATGGGGAAT TACCAAAAGC GAGATATGAA
    ATTCGTGATG ATCATACCCT GAAAATCCGG AAAGTTATGG CAGGAGACAT GGGCTCATAT
    ACTTGTGTAG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CGGCAACTCT AACAGTGCAA
    GTTGTGTCTG AGCCTCCACA GTTTGTTGTA AAGCCTCGTG ATCAGGTAGC TGCATTGGGA
    AGAACAGTGA CTTTCCAGTG TGAAGCAACT GGGAACCCAC AGCCAGCCAT CTTTTGGCGG
    AGAGAGGGAA GTCAGAATCT GCTTTTCTCA TACCAGCCCC CCCAGTCATC CAGTCGATTT
    TCAGTTTCCC AGACAGGAGA CCTCACCATC ACCAATGTCC AGAGATCTGA TGTTGGGTAC
    TACATCTGCC AGACTTTAAA TGTTGCAGGA AGCATCATAA CAAAAGCCTA CTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCT GTCATTCGAC AGGGTCCTGT GAATCAGACT
    GTAGCTGTAG ATGGTACTTT AGTACTAAAC TGTGTGGCCA CAGGCACTTT AACACCCACT
    ATCCTCTGGA AAAAGGATGG GACCCTGATC TCTACCCAGG ACTCTCGCAT CAAACAGTTG
    GAGACAGGAG CATTACAGAT CCGATATGCC AAGCTGGGTG ATACTGGTCG GTACACCTGC
    GTTGCTTCAA CCCCAAGTGG CGAAGCTACA TGGAGTGCCT ACATAGAGGT CCAAGAATTT
    GGAGTCCCAG TGCAGCCACC AAGACCCACT GACCCAAACT TGATTCCTAG TGCTCCATCA
    AAACCTGAGG TTACAGATGT CAGCAGAAAT ACAGTAACAT TGTCATGGCA ACCTAATTTG
    AATTCAGGTG CAACGCCAAC CTCTTACATA ATTGAGGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAAA CTGTAGCTGA AAATGTGAAA ACAGAGAGTT TTGCAGTTAA AGGGCTAAAA
    CCTAGTGCAA TTTATCTATT CCTTGTGAGG GCAGCTAATG CATATGGACT AAGTGACCCA
    AGCCAAATAT CTGATCCAGT GAAAACTCAA GATGTTCCAC CAACAAGTCA AGGTGTGGAT
    CACAAGCAGG TGCAGAGAGA ACTAGGCGAT GTGGTACTAC ATCTGCACAA CCCTACTATC
    CTTTCTTCCT CCTCAATTGA AGTTCATTGG ACAGTGGACC AGCAATCCCA ATATATTCAA
    GGATATAAAA TCCTATACCG GCCAACACCA GCGTCCTATG GAGAGTCAGA ATGGTTAATT
    TTTGAAGTGA GGACTCCAAC CAAAAACAGT GTTATCATTC CAGAGCTGAA GAAAGGTGTA
    AACTATGAAA TCAAGGCCCG CCCTTTTTTT AATGAGTTTC AAGGCGCAGA TAGTGAAGTG
    AAATTTGCAA AAACCCTAGA AGAAGCGCCC AGTGCTCCAC CTCAGAGTGT TTCAGTAACT
    AAAAATGATG GGAATGGAAC TGCAATTGTA GTCACCTGGC AGCCACCCCC CGAGGATAAC
    CAGAACGGAA TGGTTCAAGA ATACAAGGTT TGGTGCCTTG GCAATGAAAG TCGATACCAT
    ATAAACAGGA CAGTAGATGG TTCCACCTTT TCAGTAGTCA TCCCTTCCCT AGTACCAGGT
    ATTCGATACA GCGTGGAAGT GGCAGCAAGT ACTGGAGCAG GACCTGGAGT GAAAAGTGAT
    CCCCAGTTTA TCCAGTTAGA TTCGCATGGA AATCCTGTGT CTTCAGAAGA TCAAGTCAGT
    TTAGCCCAGC AGATCTCTGA CGTAGTAAAA CAGCCTGCCT TTATTGCTGG AATTGGTGCT
    GCTTGTTGGA TTATCCTCAT GGTCTTCAGC ATCTGGTTGT ATCGCCACAG AAAGAAGCGA
    AATGGACTCA CTAGTACTTA CGCTGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACCT ATCAGAGAGG AGGAGATGCC GTAAGCAGTG GAGGCAGGCC AGGACTCCTA
    AACATCAGTG AACCAGCCAC ACAGCCCTGG CTAGCAGATA CTTGGCCTAA CACAGGGAAC
    AACCACAATG ACTGCTCCAT CAACTGCTGC ACCTCTGGCA ATGGAAACAG TGATGGCAAC
    CTTACGACAT ACAGTCGACC AGCCGATTGT ATAGCAAATT ACAACAATCA GCTGGACAAC
    AAGCAGACAA ACCTGATGTT GCCTGAGTCA ACTGTATATG GTGACGTGGA CCTAAGCAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGT CCAAATCTAA AGGATGGAAG ATTTGTCAGC
    CAACCTGGGC AGCCCACTCC TTATGCCACC ACTCAACTCA TCCAGTCAAA TATCAGCAAT
    AATGTTAACA ATGGCAGCGG GGATACGAAT GAGAAACACT GGAAACCCTC TGTTCAGCAA
    AAGCAAGAGG TTGCACCCAT ACAGTACAAC ATTATGGAGC AAAACAAATT AAACAAAGAT
    TACCGAGTAA ATGACAGCAT TGCTCCAACA ATTCCATACA ACCAGTCATA TGACCAGAAT
    ACAGGTGGAT CCTACAACAG CTCAGATAGA GGCAGTAGCA CATCTGGGAG TCAAGGGCAC
    AAGAAGGGTG CTCGGACTCC AAAGGCTCCC AAGCAAGCTG GCATGAACTG GGCAGATCTT
    CTTCCACCCC CTCCAGCACA TCCACCTCCC CATAGCAATA GTGAAGATTA CAGTCTTTCA
    GTGGATGAAA GCTATGACCA AGAAATTCCA TGTCCAGTCC CACCTGCAAG GATGTATCTG
    CAGCAAGATG AGCTAGAGGA AGAGGAAGAA GATGAGCGAG GTCCTACTCC ACCTGTCCGA
    GGAGCAGCTT CCTCTCCAGC CGCAGTCTCC TATAGTCATC AGTCAACTGC CACTCTCACC
    CCTTCTCCCC AGGAAGAACT CCAGCCCATG TTACAGGACT GTCAGGAGGA TCTGGGACAC
    ATACAACACC AACCTGAACG GAGACGTCAG CCTGTGAGCC CTCCACCCCC TCCAAGGCCT
    ATCTCTCCAC CACATACCTA TGGATATATC TCAGGGCCTT TAGTCTCTGA CATGGATACT
    GATGCACCAG AAGAGGAAGA AGATGAGGCA GATATGGAGG TTGCTAAGAT GCAAAACAGA
    AGACTCCTTT TGCGTGGCCT TGAGCAGACA CCTGCTTCAA GTGTTGGTGA TTTGGAGAGC
    TCTGTAACAG GGTCCATGAT CAATGGCTGG GGTTCAGCTT CCGAAGAAGA TAACATCTCA
    AGTGGGCGGT CTAGTGTTAG CTCTTCAGAT GGATCATTTT TTACTGATGC AGATTTTGCA
    CAGGCTGTTG CAGCAGCTGC AGAATATGCT GGCTTGAAAG TAGCCAGACG TCAAATGCAG
    GATGCAGCAG GAGGCCGTAG ACATTTTCAT GCATCACATT GTCCCAGACC TACCAGCCCT
    GTATCTACTG ACAGCAACAT GAGTGCTGTT GTAATACAGA AGGTCCGACC TGCCAAAAAG
    CAAAAACACC AGCCAGGACA TCTGCGCAGA GAAGTCTACA CAGATGACTT GCCACCCCCT
    CCCGTGCCAC CTCCCGCTAT AAAATCACCA ACCGCCCAGT CCAAATCTCA GCTGGAGGTG
    CGACCTGTCA TGTTGCCAAA ACTGGCTTCT ATAGAACCGA GGACAGACAG ATCTGCGGAA
    AGAAAAGGAG TGAGCTACAA GGGGAGGGAC GGGGTGGATG GGAGACAGCA TTCAGACATG
    CGGACAAATT CAGGAGAGCG GAGAGAATCA CAGGAACAGC AAGGCGACGG GAAACTGCGA
    GGAAGTAAAG CACCAAAACG AGAAGGCACA CCAGCAAAAA CTCATCTTCT TCAAGAGGAC
    ATCCTGCCAT ATTCTAGACC AACGTTTCCA ACGTCAAATA ATCCCAGAGA TCCCAGCTCC
    TCCAGCTCTA TGTCTTCGAG AGGATCAGGA GGGAGACAGA GGGCAGATCA AGCAAATATA
    GCCCGAAGAA ATGTTGCAGA AATGCAAGTA CTGGGAGCAT ATGAACAAGG AGAAGAAGAA
    GAAGATGAGA TGGAGGAAAC AGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025000493.1
    CDS801..5330
    Translation

    Target ORF information:

    RefSeq Version XM_025144725.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025144725.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGTTGCGG AGTTTGCTCA CTTTTACCTC TGTGGATTAC TCTGCCTCGC CTCAGTTCTA 
    CGGGATGACT TCAGACAAAA TCCATCAGAT GTAATGGTAG CTGTGGGTGA ACCTGCTGTG
    ATGGAATGTC AGCCCCCAAG AGGTCACCCA GAACCCACTA TATCATGGAA AAAAGATGGG
    ACTCCTCTAG ATGACAAAGA TGAGAGAATT ACAATCAGAG GTGGAAAGCT TATGATCACA
    TACACAAGAA AGAATGATGC TGGCAAGTAC GTTTGTGTTG GAACAAATAT GGTTGGAGAA
    CGTGAAAGTG AAGTTGCAGA GCTCACTGTT TTAGAAAGAC CGTCTTTTGT GAAGAGACCA
    AGTAATTTGG CTGTAACTGT GGATGACAGT GCTGAGTTTA AATGTGAGGC AAGAGGTGAC
    CCAGTCCCTA CAGTAAGATG GAGGAAAGAT GATGGGGAAT TACCAAAAGC GAGATATGAA
    ATTCGTGATG ATCATACCCT GAAAATCCGG AAAGTTATGG CAGGAGACAT GGGCTCATAT
    ACTTGTGTAG CAGAAAATAT GGTTGGAAAA GCTGAAGCAT CGGCAACTCT AACAGTGCAA
    GTTGTGTCTG AGCCTCCACA GTTTGTTGTA AAGCCTCGTG ATCAGGTAGC TGCATTGGGA
    AGAACAGTGA CTTTCCAGTG TGAAGCAACT GGGAACCCAC AGCCAGCCAT CTTTTGGCGG
    AGAGAGGGAA GTCAGAATCT GCTTTTCTCA TACCAGCCCC CCCAGTCATC CAGTCGATTT
    TCAGTTTCCC AGACAGGAGA CCTCACCATC ACCAATGTCC AGAGATCTGA TGTTGGGTAC
    TACATCTGCC AGACTTTAAA TGTTGCAGGA AGCATCATAA CAAAAGCCTA CTTGGAAGTT
    ACTGATGTGA TTGCAGACCG GCCTCCCCCT GTCATTCGAC AGGGTCCTGT GAATCAGACT
    GTAGCTGTAG ATGGTACTTT AGTACTAAAC TGTGTGGCCA CAGGCACTTT AACACCCACT
    ATCCTCTGGA AAAAGGATGG GACCCTGATC TCTACCCAGG ACTCTCGCAT CAAACAGTTG
    GAGACAGGAG CATTACAGAT CCGATATGCC AAGCTGGGTG ATACTGGTCG GTACACCTGC
    GTTGCTTCAA CCCCAAGTGG CGAAGCTACA TGGAGTGCCT ACATAGAGGT CCAAGAATTT
    GGAGTCCCAG TGCAGCCACC AAGACCCACT GACCCAAACT TGATTCCTAG TGCTCCATCA
    AAACCTGAGG TTACAGATGT CAGCAGAAAT ACAGTAACAT TGTCATGGCA ACCTAATTTG
    AATTCAGGTG CAACGCCAAC CTCTTACATA ATTGAGGCCT TCAGCCATGC ATCTGGTAGC
    AGCTGGCAAA CTGTAGCTGA AAATGTGAAA ACAGAGAGTT TTGCAGTTAA AGGGCTAAAA
    CCTAGTGCAA TTTATCTATT CCTTGTGAGG GCAGCTAATG CATATGGACT AAGTGACCCA
    AGCCAAATAT CTGATCCAGT GAAAACTCAA GATGTTCCAC CAACAAGTCA AGGTGTGGAT
    CACAAGCAGG TGCAGAGAGA ACTAGGCGAT GTGGTACTAC ATCTGCACAA CCCTACTATC
    CTTTCTTCCT CCTCAATTGA AGTTCATTGG ACAGTGGACC AGCAATCCCA ATATATTCAA
    GGATATAAAA TCCTATACCG GCCAACACCA GCGTCCTATG GAGAGTCAGA ATGGTTAATT
    TTTGAAGTGA GGACTCCAAC CAAAAACAGT GTTATCATTC CAGAGCTGAA GAAAGGTGTA
    AACTATGAAA TCAAGGCCCG CCCTTTTTTT AATGAGTTTC AAGGCGCAGA TAGTGAAGTG
    AAATTTGCAA AAACCCTAGA AGAAGCGCCC AGTGCTCCAC CTCAGAGTGT TTCAGTAACT
    AAAAATGATG GGAATGGAAC TGCAATTGTA GTCACCTGGC AGCCACCCCC CGAGGATAAC
    CAGAACGGAA TGGTTCAAGA ATACAAGGTT TGGTGCCTTG GCAATGAAAG TCGATACCAT
    ATAAACAGGA CAGTAGATGG TTCCACCTTT TCAGTAGTCA TCCCTTCCCT AGTACCAGGT
    ATTCGATACA GCGTGGAAGT GGCAGCAAGT ACTGGAGCAG GACCTGGAGT GAAAAGTGAT
    CCCCAGTTTA TCCAGTTAGA TTCGCATGGA AATCCTGTGT CTTCAGAAGA TCAAGTCAGT
    TTAGCCCAGC AGATCTCTGA CGTAGTAAAA CAGCCTGCCT TTATTGCTGG AATTGGTGCT
    GCTTGTTGGA TTATCCTCAT GGTCTTCAGC ATCTGGTTGT ATCGCCACAG AAAGAAGCGA
    AATGGACTCA CTAGTACTTA CGCTGGTATC AGAAAAGTCC CGTCTTTTAC CTTCACACCA
    ACAGTAACCT ATCAGAGAGG AGGAGATGCC GTAAGCAGTG GAGGCAGGCC AGGACTCCTA
    AACATCAGTG AACCAGCCAC ACAGCCCTGG CTAGCAGATA CTTGGCCTAA CACAGGGAAC
    AACCACAATG ACTGCTCCAT CAACTGCTGC ACCTCTGGCA ATGGAAACAG TGATGGCAAC
    CTTACGACAT ACAGTCGACC AGCCGATTGT ATAGCAAATT ACAACAATCA GCTGGACAAC
    AAGCAGACAA ACCTGATGTT GCCTGAGTCA ACTGTATATG GTGACGTGGA CCTAAGCAAC
    AAAATCAATG AGATGAAAAC CTTCAATAGT CCAAATCTAA AGGATGGAAG ATTTGTCAGC
    CAACCTGGGC AGCCCACTCC TTATGCCACC ACTCAACTCA TCCAGTCAAA TATCAGCAAT
    AATGTTAACA ATGGCAGCGG GGATACGAAT GAGAAACACT GGAAACCCTC TGTTCAGCAA
    AAGCAAGAGG TTGCACCCAT ACAGTACAAC ATTATGGAGC AAAACAAATT AAACAAAGAT
    TACCGAGTAA ATGACAGCAT TGCTCCAACA ATTCCATACA ACCAGTCATA TGACCAGAAT
    ACAGGTGGAT CCTACAACAG CTCAGATAGA GGCAGTAGCA CATCTGGGAG TCAAGGGCAC
    AAGAAGGGTG CTCGGACTCC AAAGGCTCCC AAGCAAGCTG GCATGAACTG GGCAGATCTT
    CTTCCACCCC CTCCAGCACA TCCACCTCCC CATAGCAATA GTGAAGATTA CAGTCTTTCA
    GTGGATGAAA GCTATGACCA AGAAATTCCA TGTCCAGTCC CACCTGCAAG GATGTATCTG
    CAGCAAGATG AGCTAGAGGA AGAGGAAGAA GATGAGCGAG GTCCTACTCC ACCTGTCCGA
    GGAGCAGCTT CCTCTCCAGC CGCAGTCTCC TATAGTCATC AGTCAACTGC CACTCTCACC
    CCTTCTCCCC AGGAAGAACT CCAGCCCATG TTACAGGACT GTCAGGAGGA TCTGGGACAC
    ATACAACACC AACCTGAACG GAGACGTCAG CCTGTGAGCC CTCCACCCCC TCCAAGGCCT
    ATCTCTCCAC CACATACCTA TGGATATATC TCAGGGCCTT TAGTCTCTGA CATGGATACT
    GATGCACCAG AAGAGGAAGA AGATGAGGCA GATATGGAGG TTGCTAAGAT GCAAAACAGA
    AGACTCCTTT TGCGTGGCCT TGAGCAGACA CCTGCTTCAA GTGTTGGTGA TTTGGAGAGC
    TCTGTAACAG GGTCCATGAT CAATGGCTGG GGTTCAGCTT CCGAAGAAGA TAACATCTCA
    AGTGGGCGGT CTAGTGTTAG CTCTTCAGAT GGATCATTTT TTACTGATGC AGATTTTGCA
    CAGGCTGTTG CAGCAGCTGC AGAATATGCT GGCTTGAAAG TAGCCAGACG TCAAATGCAG
    GATGCAGCAG GAGGCCGTAG ACATTTTCAT GCATCACATT GTCCCAGACC TACCAGCCCT
    GTATCTACTG ACAGCAACAT GAGTGCTGTT GTAATACAGA AGGTCCGACC TGCCAAAAAG
    CAAAAACACC AGCCAGGACA TCTGCGCAGA GAAGTCTACA CAGATGACTT GCCACCCCCT
    CCCGTGCCAC CTCCCGCTAT AAAATCACCA ACCGCCCAGT CCAAATCTCA GCTGGAGGTG
    CGACCTGTCA TGTTGCCAAA ACTGGCTTCT ATAGAACCGA GGACAGACAG ATCTGCGGAA
    AGAAAAGGAG TGAGCTACAA GGGGAGGGAC GGGGTGGATG GGAGACAGCA TTCAGACATG
    CGGACAAATT CAGGAGAGCG GAGAGAATCA CAGGAACAGC AAGGCGACGG GAAACTGCGA
    GGAAGTAAAG CACCAAAACG AGAAGGCACA CCAGCAAAAA CTCATCTTCT TCAAGAGGAC
    ATCCTGCCAT ATTCTAGACC AACGTTTCCA ACGTCAAATA ATCCCAGAGA TCCCAGCTCC
    TCCAGCTCTA TGTCTTCGAG AGGATCAGGA GGGAGACAGA GGGCAGATCA AGCAAATATA
    GCCCGAAGAA ATGTTGCAGA AATGCAAGTA CTGGGAGCAT ATGAACAAGG AGAAGAAGAA
    GAAGATGAGA TGGAGGAAAC AGAAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30202
    Clone ID Related Accession (Same CDS sequence) XM_015298481.2 , XM_015298481.1
    Accession Version XM_015298481.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGAGCCT CCACAGTTTG TTGTAAAGCC TCGTGATCAG
    GTAGCTGCAT TGGGAAGAAC AGTGACTTTC CAGTGTGAAG CAACTGGGAA CCCACAGCCA
    GCCATCTTTT GGCGGAGAGA GGGAAGTCAG AATCTGCTTT TCTCATACCA GCCCCCCCAG
    TCATCCAGTC GATTTTCAGT TTCCCAGACA GGAGACCTCA CCATCACCAA TGTCCAGAGA
    TCTGATGTTG GGTACTACAT CTGCCAGACT TTAAATGTTG CAGGAAGCAT CATAACAAAA
    GCCTACTTGG AAGTTACTGA TGTGATTGCA GACCGGCCTC CCCCTGTCAT TCGACAGGGT
    CCTGTGAATC AGACTGTAGC TGTAGATGGT ACTTTAGTAC TAAACTGTGT GGCCACAGGC
    ACTTTAACAC CCACTATCCT CTGGAAAAAG GATGGGACCC TGATCTCTAC CCAGGACTCT
    CGCATCAAAC AGTTGGAGAC AGGAGCATTA CAGATCCGAT ATGCCAAGCT GGGTGATACT
    GGTCGGTACA CCTGCGTTGC TTCAACCCCA AGTGGCGAAG CTACATGGAG TGCCTACATA
    GAGGTCCAAG AATTTGGAGT CCCAGTGCAG CCACCAAGAC CCACTGACCC AAACTTGATT
    CCTAGTGCTC CATCAAAACC TGAGGTTACA GATGTCAGCA GAAATACAGT AACATTGTCA
    TGGCAACCTA ATTTGAATTC AGGTGCAACG CCAACCTCTT ACATAATTGA GGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAAACTGTA GCTGAAAATG TGAAAACAGA GAGTTTTGCA
    GTTAAAGGGC TAAAACCTAG TGCAATTTAT CTATTCCTTG TGAGGGCAGC TAATGCATAT
    GGACTAAGTG ACCCAAGCCA AATATCTGAT CCAGTGAAAA CTCAAGATGT TCCACCAACA
    AGTCAAGGTG TGGATCACAA GCAGGTGCAG AGAGAACTAG GCGATGTGGT ACTACATCTG
    CACAACCCTA CTATCCTTTC TTCCTCCTCA ATTGAAGTTC ATTGGACAGT GGACCAGCAA
    TCCCAATATA TTCAAGGATA TAAAATCCTA TACCGGCCAA CACCAGCGTC CTATGGAGAG
    TCAGAATGGT TAATTTTTGA AGTGAGGACT CCAACCAAAA ACAGTGTTAT CATTCCAGAG
    CTGAAGAAAG GTGTAAACTA TGAAATCAAG GCCCGCCCTT TTTTTAATGA GTTTCAAGGC
    GCAGATAGTG AAGTGAAATT TGCAAAAACC CTAGAAGAAG CGCCCAGTGC TCCACCTCAG
    AGTGTTTCAG TAACTAAAAA TGATGGGAAT GGAACTGCAA TTGTAGTCAC CTGGCAGCCA
    CCCCCCGAGG ATAACCAGAA CGGAATGGTT CAAGAATACA AGGTTTGGTG CCTTGGCAAT
    GAAAGTCGAT ACCATATAAA CAGGACAGTA GATGGTTCCA CCTTTTCAGT AGTCATCCCT
    TCCCTAGTAC CAGGTATTCG ATACAGCGTG GAAGTGGCAG CAAGTACTGG AGCAGGACCT
    GGAGTGAAAA GTGATCCCCA GTTTATCCAG TTAGATTCGC ATGGAAATCC TGTGTCTTCA
    GAAGATCAAG TCAGTTTAGC CCAGCAGATC TCTGACGTAG TAAAACAGCC TGCCTTTATT
    GCTGGAATTG GTGCTGCTTG TTGGATTATC CTCATGGTCT TCAGCATCTG GTTGTATCGC
    CACAGAAAGA AGCGAAATGG ACTCACTAGT ACTTACGCTG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACCTATCAG AGAGGAGGAG ATGCCGTAAG CAGTGGAGGC
    AGGCCAGGAC TCCTAAACAT CAGTGAACCA GCCACACAGC CCTGGCTAGC AGATACTTGG
    CCTAACACAG GGAACAACCA CAATGACTGC TCCATCAACT GCTGCACCTC TGGCAATGGA
    AACAGTGATG GCAACCTTAC GACATACAGT CGACCAGCCG ATTGTATAGC AAATTACAAC
    AATCAGCTGG ACAACAAGCA GACAAACCTG ATGTTGCCTG AGTCAACTGT ATATGGTGAC
    GTGGACCTAA GCAACAAAAT CAATGAGATG AAAACCTTCA ATAGTCCAAA TCTAAAGGAT
    GGAAGATTTG TCAGCCAACC TGGGCAGCCC ACTCCTTATG CCACCACTCA ACTCATCCAG
    TCAAATATCA GCAATAATGT TAACAATGGC AGCGGGGATA CGAATGAGAA ACACTGGAAA
    CCCTCTGTTC AGCAAAAGCA AGAGGTTGCA CCCATACAGT ACAACATTAT GGAGCAAAAC
    AAATTAAACA AAGATTACCG AGTAAATGAC AGCATTGCTC CAACAATTCC ATACAACCAG
    TCATATGACC AGAATACAGG TGGATCCTAC AACAGCTCAG ATAGAGGCAG TAGCACATCT
    GGGAGTCAAG GGCACAAGAA GGGTGCTCGG ACTCCAAAGG CTCCCAAGCA AGCTGGCATG
    AACTGGGCAG ATCTTCTTCC ACCCCCTCCA GCACATCCAC CTCCCCATAG CAATAGTGAA
    GATTACAGTC TTTCAGTGGA TGAAAGCTAT GACCAAGAAA TTCCATGTCC AGTCCCACCT
    GCAAGGATGT ATCTGCAGCA AGATGAGCTA GAGGAAGAGG AAGAAGATGA GCGAGGTCCT
    ACTCCACCTG TCCGAGGAGC AGCTTCCTCT CCAGCCGCAG TCTCCTATAG TCATCAGTCA
    ACTGCCACTC TCACCCCTTC TCCCCAGGAA GAACTCCAGC CCATGTTACA GGACTGTCAG
    GAGGATCTGG GACACATACA ACACCAACCT GAACGGAGAC GTCAGCCTGT GAGCCCTCCA
    CCCCCTCCAA GGCCTATCTC TCCACCACAT ACCTATGGAT ATATCTCAGG GCCTTTAGTC
    TCTGACATGG ATACTGATGC ACCAGAAGAG GAAGAAGATG AGGCAGATAT GGAGGTTGCT
    AAGATGCAAA ACAGAAGACT CCTTTTGCGT GGCCTTGAGC AGACACCTGC TTCAAGTGTT
    GGTGATTTGG AGAGCTCTGT AACAGGGTCC ATGATCAATG GCTGGGGTTC AGCTTCCGAA
    GAAGATAACA TCTCAAGTGG GCGGTCTAGT GTTAGCTCTT CAGATGGATC ATTTTTTACT
    GATGCAGATT TTGCACAGGC TGTTGCAGCA GCTGCAGAAT ATGCTGGCTT GAAAGTAGCC
    AGACGTCAAA TGCAGGATGC AGCAGGAGGC CGTAGACATT TTCATGCATC ACATTGTCCC
    AGACCTACCA GCCCTGTATC TACTGACAGC AACATGAGTG CTGTTGTAAT ACAGAAGGTC
    CGACCTGCCA AAAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GACTTGCCAC CCCCTCCCGT GCCACCTCCC GCTATAAAAT CACCAACCGC CCAGTCCAAA
    TCTCAGCTGG AGGTGCGACC TGTCATGTTG CCAAAACTGG CTTCTATAGA ACCGAGGACA
    GACAGATCTG CGGAAAGAAA AGGAGTGAGC TACAAGGGGA GGGACGGGGT GGATGGGAGA
    CAGCATTCAG ACATGCGGAC AAATTCAGGA GAGCGGAGAG AATCACAGGA ACAGCAAGGC
    GACGGGAAAC TGCGAGGAAG TAAAGCACCA AAACGAGAAG GCACACCAGC AAAAACTCAT
    CTTCTTCAAG AGGACATCCT GCCATATTCT AGACCAACGT TTCCAACGTC AAATAATCCC
    AGAGATCCCA GCTCCTCCAG CTCTATGTCT TCGAGAGGAT CAGGAGGGAG ACAGAGGGCA
    GATCAAGCAA ATATAGCCCG AAGAAATGTT GCAGAAATGC AAGTACTGGG AGCATATGAA
    CAAGGAGAAG AAGAAGAAGA TGAGATGGAG GAAACAGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153967.1
    CDS326..5290
    Translation

    Target ORF information:

    RefSeq Version XM_015298481.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298481.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGAGCCT CCACAGTTTG TTGTAAAGCC TCGTGATCAG
    GTAGCTGCAT TGGGAAGAAC AGTGACTTTC CAGTGTGAAG CAACTGGGAA CCCACAGCCA
    GCCATCTTTT GGCGGAGAGA GGGAAGTCAG AATCTGCTTT TCTCATACCA GCCCCCCCAG
    TCATCCAGTC GATTTTCAGT TTCCCAGACA GGAGACCTCA CCATCACCAA TGTCCAGAGA
    TCTGATGTTG GGTACTACAT CTGCCAGACT TTAAATGTTG CAGGAAGCAT CATAACAAAA
    GCCTACTTGG AAGTTACTGA TGTGATTGCA GACCGGCCTC CCCCTGTCAT TCGACAGGGT
    CCTGTGAATC AGACTGTAGC TGTAGATGGT ACTTTAGTAC TAAACTGTGT GGCCACAGGC
    ACTTTAACAC CCACTATCCT CTGGAAAAAG GATGGGACCC TGATCTCTAC CCAGGACTCT
    CGCATCAAAC AGTTGGAGAC AGGAGCATTA CAGATCCGAT ATGCCAAGCT GGGTGATACT
    GGTCGGTACA CCTGCGTTGC TTCAACCCCA AGTGGCGAAG CTACATGGAG TGCCTACATA
    GAGGTCCAAG AATTTGGAGT CCCAGTGCAG CCACCAAGAC CCACTGACCC AAACTTGATT
    CCTAGTGCTC CATCAAAACC TGAGGTTACA GATGTCAGCA GAAATACAGT AACATTGTCA
    TGGCAACCTA ATTTGAATTC AGGTGCAACG CCAACCTCTT ACATAATTGA GGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAAACTGTA GCTGAAAATG TGAAAACAGA GAGTTTTGCA
    GTTAAAGGGC TAAAACCTAG TGCAATTTAT CTATTCCTTG TGAGGGCAGC TAATGCATAT
    GGACTAAGTG ACCCAAGCCA AATATCTGAT CCAGTGAAAA CTCAAGATGT TCCACCAACA
    AGTCAAGGTG TGGATCACAA GCAGGTGCAG AGAGAACTAG GCGATGTGGT ACTACATCTG
    CACAACCCTA CTATCCTTTC TTCCTCCTCA ATTGAAGTTC ATTGGACAGT GGACCAGCAA
    TCCCAATATA TTCAAGGATA TAAAATCCTA TACCGGCCAA CACCAGCGTC CTATGGAGAG
    TCAGAATGGT TAATTTTTGA AGTGAGGACT CCAACCAAAA ACAGTGTTAT CATTCCAGAG
    CTGAAGAAAG GTGTAAACTA TGAAATCAAG GCCCGCCCTT TTTTTAATGA GTTTCAAGGC
    GCAGATAGTG AAGTGAAATT TGCAAAAACC CTAGAAGAAG CGCCCAGTGC TCCACCTCAG
    AGTGTTTCAG TAACTAAAAA TGATGGGAAT GGAACTGCAA TTGTAGTCAC CTGGCAGCCA
    CCCCCCGAGG ATAACCAGAA CGGAATGGTT CAAGAATACA AGGTTTGGTG CCTTGGCAAT
    GAAAGTCGAT ACCATATAAA CAGGACAGTA GATGGTTCCA CCTTTTCAGT AGTCATCCCT
    TCCCTAGTAC CAGGTATTCG ATACAGCGTG GAAGTGGCAG CAAGTACTGG AGCAGGACCT
    GGAGTGAAAA GTGATCCCCA GTTTATCCAG TTAGATTCGC ATGGAAATCC TGTGTCTTCA
    GAAGATCAAG TCAGTTTAGC CCAGCAGATC TCTGACGTAG TAAAACAGCC TGCCTTTATT
    GCTGGAATTG GTGCTGCTTG TTGGATTATC CTCATGGTCT TCAGCATCTG GTTGTATCGC
    CACAGAAAGA AGCGAAATGG ACTCACTAGT ACTTACGCTG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACCTATCAG AGAGGAGGAG ATGCCGTAAG CAGTGGAGGC
    AGGCCAGGAC TCCTAAACAT CAGTGAACCA GCCACACAGC CCTGGCTAGC AGATACTTGG
    CCTAACACAG GGAACAACCA CAATGACTGC TCCATCAACT GCTGCACCTC TGGCAATGGA
    AACAGTGATG GCAACCTTAC GACATACAGT CGACCAGCCG ATTGTATAGC AAATTACAAC
    AATCAGCTGG ACAACAAGCA GACAAACCTG ATGTTGCCTG AGTCAACTGT ATATGGTGAC
    GTGGACCTAA GCAACAAAAT CAATGAGATG AAAACCTTCA ATAGTCCAAA TCTAAAGGAT
    GGAAGATTTG TCAGCCAACC TGGGCAGCCC ACTCCTTATG CCACCACTCA ACTCATCCAG
    TCAAATATCA GCAATAATGT TAACAATGGC AGCGGGGATA CGAATGAGAA ACACTGGAAA
    CCCTCTGTTC AGCAAAAGCA AGAGGTTGCA CCCATACAGT ACAACATTAT GGAGCAAAAC
    AAATTAAACA AAGATTACCG AGTAAATGAC AGCATTGCTC CAACAATTCC ATACAACCAG
    TCATATGACC AGAATACAGG TGGATCCTAC AACAGCTCAG ATAGAGGCAG TAGCACATCT
    GGGAGTCAAG GGCACAAGAA GGGTGCTCGG ACTCCAAAGG CTCCCAAGCA AGCTGGCATG
    AACTGGGCAG ATCTTCTTCC ACCCCCTCCA GCACATCCAC CTCCCCATAG CAATAGTGAA
    GATTACAGTC TTTCAGTGGA TGAAAGCTAT GACCAAGAAA TTCCATGTCC AGTCCCACCT
    GCAAGGATGT ATCTGCAGCA AGATGAGCTA GAGGAAGAGG AAGAAGATGA GCGAGGTCCT
    ACTCCACCTG TCCGAGGAGC AGCTTCCTCT CCAGCCGCAG TCTCCTATAG TCATCAGTCA
    ACTGCCACTC TCACCCCTTC TCCCCAGGAA GAACTCCAGC CCATGTTACA GGACTGTCAG
    GAGGATCTGG GACACATACA ACACCAACCT GAACGGAGAC GTCAGCCTGT GAGCCCTCCA
    CCCCCTCCAA GGCCTATCTC TCCACCACAT ACCTATGGAT ATATCTCAGG GCCTTTAGTC
    TCTGACATGG ATACTGATGC ACCAGAAGAG GAAGAAGATG AGGCAGATAT GGAGGTTGCT
    AAGATGCAAA ACAGAAGACT CCTTTTGCGT GGCCTTGAGC AGACACCTGC TTCAAGTGTT
    GGTGATTTGG AGAGCTCTGT AACAGGGTCC ATGATCAATG GCTGGGGTTC AGCTTCCGAA
    GAAGATAACA TCTCAAGTGG GCGGTCTAGT GTTAGCTCTT CAGATGGATC ATTTTTTACT
    GATGCAGATT TTGCACAGGC TGTTGCAGCA GCTGCAGAAT ATGCTGGCTT GAAAGTAGCC
    AGACGTCAAA TGCAGGATGC AGCAGGAGGC CGTAGACATT TTCATGCATC ACATTGTCCC
    AGACCTACCA GCCCTGTATC TACTGACAGC AACATGAGTG CTGTTGTAAT ACAGAAGGTC
    CGACCTGCCA AAAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GACTTGCCAC CCCCTCCCGT GCCACCTCCC GCTATAAAAT CACCAACCGC CCAGTCCAAA
    TCTCAGCTGG AGGTGCGACC TGTCATGTTG CCAAAACTGG CTTCTATAGA ACCGAGGACA
    GACAGATCTG CGGAAAGAAA AGGAGTGAGC TACAAGGGGA GGGACGGGGT GGATGGGAGA
    CAGCATTCAG ACATGCGGAC AAATTCAGGA GAGCGGAGAG AATCACAGGA ACAGCAAGGC
    GACGGGAAAC TGCGAGGAAG TAAAGCACCA AAACGAGAAG GCACACCAGC AAAAACTCAT
    CTTCTTCAAG AGGACATCCT GCCATATTCT AGACCAACGT TTCCAACGTC AAATAATCCC
    AGAGATCCCA GCTCCTCCAG CTCTATGTCT TCGAGAGGAT CAGGAGGGAG ACAGAGGGCA
    GATCAAGCAA ATATAGCCCG AAGAAATGTT GCAGAAATGC AAGTACTGGG AGCATATGAA
    CAAGGAGAAG AAGAAGAAGA TGAGATGGAG GAAACAGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30202
    Clone ID Related Accession (Same CDS sequence) XM_015298481.2 , XM_015298481.1
    Accession Version XM_015298481.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGAGCCT CCACAGTTTG TTGTAAAGCC TCGTGATCAG
    GTAGCTGCAT TGGGAAGAAC AGTGACTTTC CAGTGTGAAG CAACTGGGAA CCCACAGCCA
    GCCATCTTTT GGCGGAGAGA GGGAAGTCAG AATCTGCTTT TCTCATACCA GCCCCCCCAG
    TCATCCAGTC GATTTTCAGT TTCCCAGACA GGAGACCTCA CCATCACCAA TGTCCAGAGA
    TCTGATGTTG GGTACTACAT CTGCCAGACT TTAAATGTTG CAGGAAGCAT CATAACAAAA
    GCCTACTTGG AAGTTACTGA TGTGATTGCA GACCGGCCTC CCCCTGTCAT TCGACAGGGT
    CCTGTGAATC AGACTGTAGC TGTAGATGGT ACTTTAGTAC TAAACTGTGT GGCCACAGGC
    ACTTTAACAC CCACTATCCT CTGGAAAAAG GATGGGACCC TGATCTCTAC CCAGGACTCT
    CGCATCAAAC AGTTGGAGAC AGGAGCATTA CAGATCCGAT ATGCCAAGCT GGGTGATACT
    GGTCGGTACA CCTGCGTTGC TTCAACCCCA AGTGGCGAAG CTACATGGAG TGCCTACATA
    GAGGTCCAAG AATTTGGAGT CCCAGTGCAG CCACCAAGAC CCACTGACCC AAACTTGATT
    CCTAGTGCTC CATCAAAACC TGAGGTTACA GATGTCAGCA GAAATACAGT AACATTGTCA
    TGGCAACCTA ATTTGAATTC AGGTGCAACG CCAACCTCTT ACATAATTGA GGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAAACTGTA GCTGAAAATG TGAAAACAGA GAGTTTTGCA
    GTTAAAGGGC TAAAACCTAG TGCAATTTAT CTATTCCTTG TGAGGGCAGC TAATGCATAT
    GGACTAAGTG ACCCAAGCCA AATATCTGAT CCAGTGAAAA CTCAAGATGT TCCACCAACA
    AGTCAAGGTG TGGATCACAA GCAGGTGCAG AGAGAACTAG GCGATGTGGT ACTACATCTG
    CACAACCCTA CTATCCTTTC TTCCTCCTCA ATTGAAGTTC ATTGGACAGT GGACCAGCAA
    TCCCAATATA TTCAAGGATA TAAAATCCTA TACCGGCCAA CACCAGCGTC CTATGGAGAG
    TCAGAATGGT TAATTTTTGA AGTGAGGACT CCAACCAAAA ACAGTGTTAT CATTCCAGAG
    CTGAAGAAAG GTGTAAACTA TGAAATCAAG GCCCGCCCTT TTTTTAATGA GTTTCAAGGC
    GCAGATAGTG AAGTGAAATT TGCAAAAACC CTAGAAGAAG CGCCCAGTGC TCCACCTCAG
    AGTGTTTCAG TAACTAAAAA TGATGGGAAT GGAACTGCAA TTGTAGTCAC CTGGCAGCCA
    CCCCCCGAGG ATAACCAGAA CGGAATGGTT CAAGAATACA AGGTTTGGTG CCTTGGCAAT
    GAAAGTCGAT ACCATATAAA CAGGACAGTA GATGGTTCCA CCTTTTCAGT AGTCATCCCT
    TCCCTAGTAC CAGGTATTCG ATACAGCGTG GAAGTGGCAG CAAGTACTGG AGCAGGACCT
    GGAGTGAAAA GTGATCCCCA GTTTATCCAG TTAGATTCGC ATGGAAATCC TGTGTCTTCA
    GAAGATCAAG TCAGTTTAGC CCAGCAGATC TCTGACGTAG TAAAACAGCC TGCCTTTATT
    GCTGGAATTG GTGCTGCTTG TTGGATTATC CTCATGGTCT TCAGCATCTG GTTGTATCGC
    CACAGAAAGA AGCGAAATGG ACTCACTAGT ACTTACGCTG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACCTATCAG AGAGGAGGAG ATGCCGTAAG CAGTGGAGGC
    AGGCCAGGAC TCCTAAACAT CAGTGAACCA GCCACACAGC CCTGGCTAGC AGATACTTGG
    CCTAACACAG GGAACAACCA CAATGACTGC TCCATCAACT GCTGCACCTC TGGCAATGGA
    AACAGTGATG GCAACCTTAC GACATACAGT CGACCAGCCG ATTGTATAGC AAATTACAAC
    AATCAGCTGG ACAACAAGCA GACAAACCTG ATGTTGCCTG AGTCAACTGT ATATGGTGAC
    GTGGACCTAA GCAACAAAAT CAATGAGATG AAAACCTTCA ATAGTCCAAA TCTAAAGGAT
    GGAAGATTTG TCAGCCAACC TGGGCAGCCC ACTCCTTATG CCACCACTCA ACTCATCCAG
    TCAAATATCA GCAATAATGT TAACAATGGC AGCGGGGATA CGAATGAGAA ACACTGGAAA
    CCCTCTGTTC AGCAAAAGCA AGAGGTTGCA CCCATACAGT ACAACATTAT GGAGCAAAAC
    AAATTAAACA AAGATTACCG AGTAAATGAC AGCATTGCTC CAACAATTCC ATACAACCAG
    TCATATGACC AGAATACAGG TGGATCCTAC AACAGCTCAG ATAGAGGCAG TAGCACATCT
    GGGAGTCAAG GGCACAAGAA GGGTGCTCGG ACTCCAAAGG CTCCCAAGCA AGCTGGCATG
    AACTGGGCAG ATCTTCTTCC ACCCCCTCCA GCACATCCAC CTCCCCATAG CAATAGTGAA
    GATTACAGTC TTTCAGTGGA TGAAAGCTAT GACCAAGAAA TTCCATGTCC AGTCCCACCT
    GCAAGGATGT ATCTGCAGCA AGATGAGCTA GAGGAAGAGG AAGAAGATGA GCGAGGTCCT
    ACTCCACCTG TCCGAGGAGC AGCTTCCTCT CCAGCCGCAG TCTCCTATAG TCATCAGTCA
    ACTGCCACTC TCACCCCTTC TCCCCAGGAA GAACTCCAGC CCATGTTACA GGACTGTCAG
    GAGGATCTGG GACACATACA ACACCAACCT GAACGGAGAC GTCAGCCTGT GAGCCCTCCA
    CCCCCTCCAA GGCCTATCTC TCCACCACAT ACCTATGGAT ATATCTCAGG GCCTTTAGTC
    TCTGACATGG ATACTGATGC ACCAGAAGAG GAAGAAGATG AGGCAGATAT GGAGGTTGCT
    AAGATGCAAA ACAGAAGACT CCTTTTGCGT GGCCTTGAGC AGACACCTGC TTCAAGTGTT
    GGTGATTTGG AGAGCTCTGT AACAGGGTCC ATGATCAATG GCTGGGGTTC AGCTTCCGAA
    GAAGATAACA TCTCAAGTGG GCGGTCTAGT GTTAGCTCTT CAGATGGATC ATTTTTTACT
    GATGCAGATT TTGCACAGGC TGTTGCAGCA GCTGCAGAAT ATGCTGGCTT GAAAGTAGCC
    AGACGTCAAA TGCAGGATGC AGCAGGAGGC CGTAGACATT TTCATGCATC ACATTGTCCC
    AGACCTACCA GCCCTGTATC TACTGACAGC AACATGAGTG CTGTTGTAAT ACAGAAGGTC
    CGACCTGCCA AAAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GACTTGCCAC CCCCTCCCGT GCCACCTCCC GCTATAAAAT CACCAACCGC CCAGTCCAAA
    TCTCAGCTGG AGGTGCGACC TGTCATGTTG CCAAAACTGG CTTCTATAGA ACCGAGGACA
    GACAGATCTG CGGAAAGAAA AGGAGTGAGC TACAAGGGGA GGGACGGGGT GGATGGGAGA
    CAGCATTCAG ACATGCGGAC AAATTCAGGA GAGCGGAGAG AATCACAGGA ACAGCAAGGC
    GACGGGAAAC TGCGAGGAAG TAAAGCACCA AAACGAGAAG GCACACCAGC AAAAACTCAT
    CTTCTTCAAG AGGACATCCT GCCATATTCT AGACCAACGT TTCCAACGTC AAATAATCCC
    AGAGATCCCA GCTCCTCCAG CTCTATGTCT TCGAGAGGAT CAGGAGGGAG ACAGAGGGCA
    GATCAAGCAA ATATAGCCCG AAGAAATGTT GCAGAAATGC AAGTACTGGG AGCATATGAA
    CAAGGAGAAG AAGAAGAAGA TGAGATGGAG GAAACAGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153967.1
    CDS324..5288
    Translation

    Target ORF information:

    RefSeq Version XM_015298481.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298481.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGAGCCT CCACAGTTTG TTGTAAAGCC TCGTGATCAG
    GTAGCTGCAT TGGGAAGAAC AGTGACTTTC CAGTGTGAAG CAACTGGGAA CCCACAGCCA
    GCCATCTTTT GGCGGAGAGA GGGAAGTCAG AATCTGCTTT TCTCATACCA GCCCCCCCAG
    TCATCCAGTC GATTTTCAGT TTCCCAGACA GGAGACCTCA CCATCACCAA TGTCCAGAGA
    TCTGATGTTG GGTACTACAT CTGCCAGACT TTAAATGTTG CAGGAAGCAT CATAACAAAA
    GCCTACTTGG AAGTTACTGA TGTGATTGCA GACCGGCCTC CCCCTGTCAT TCGACAGGGT
    CCTGTGAATC AGACTGTAGC TGTAGATGGT ACTTTAGTAC TAAACTGTGT GGCCACAGGC
    ACTTTAACAC CCACTATCCT CTGGAAAAAG GATGGGACCC TGATCTCTAC CCAGGACTCT
    CGCATCAAAC AGTTGGAGAC AGGAGCATTA CAGATCCGAT ATGCCAAGCT GGGTGATACT
    GGTCGGTACA CCTGCGTTGC TTCAACCCCA AGTGGCGAAG CTACATGGAG TGCCTACATA
    GAGGTCCAAG AATTTGGAGT CCCAGTGCAG CCACCAAGAC CCACTGACCC AAACTTGATT
    CCTAGTGCTC CATCAAAACC TGAGGTTACA GATGTCAGCA GAAATACAGT AACATTGTCA
    TGGCAACCTA ATTTGAATTC AGGTGCAACG CCAACCTCTT ACATAATTGA GGCCTTCAGC
    CATGCATCTG GTAGCAGCTG GCAAACTGTA GCTGAAAATG TGAAAACAGA GAGTTTTGCA
    GTTAAAGGGC TAAAACCTAG TGCAATTTAT CTATTCCTTG TGAGGGCAGC TAATGCATAT
    GGACTAAGTG ACCCAAGCCA AATATCTGAT CCAGTGAAAA CTCAAGATGT TCCACCAACA
    AGTCAAGGTG TGGATCACAA GCAGGTGCAG AGAGAACTAG GCGATGTGGT ACTACATCTG
    CACAACCCTA CTATCCTTTC TTCCTCCTCA ATTGAAGTTC ATTGGACAGT GGACCAGCAA
    TCCCAATATA TTCAAGGATA TAAAATCCTA TACCGGCCAA CACCAGCGTC CTATGGAGAG
    TCAGAATGGT TAATTTTTGA AGTGAGGACT CCAACCAAAA ACAGTGTTAT CATTCCAGAG
    CTGAAGAAAG GTGTAAACTA TGAAATCAAG GCCCGCCCTT TTTTTAATGA GTTTCAAGGC
    GCAGATAGTG AAGTGAAATT TGCAAAAACC CTAGAAGAAG CGCCCAGTGC TCCACCTCAG
    AGTGTTTCAG TAACTAAAAA TGATGGGAAT GGAACTGCAA TTGTAGTCAC CTGGCAGCCA
    CCCCCCGAGG ATAACCAGAA CGGAATGGTT CAAGAATACA AGGTTTGGTG CCTTGGCAAT
    GAAAGTCGAT ACCATATAAA CAGGACAGTA GATGGTTCCA CCTTTTCAGT AGTCATCCCT
    TCCCTAGTAC CAGGTATTCG ATACAGCGTG GAAGTGGCAG CAAGTACTGG AGCAGGACCT
    GGAGTGAAAA GTGATCCCCA GTTTATCCAG TTAGATTCGC ATGGAAATCC TGTGTCTTCA
    GAAGATCAAG TCAGTTTAGC CCAGCAGATC TCTGACGTAG TAAAACAGCC TGCCTTTATT
    GCTGGAATTG GTGCTGCTTG TTGGATTATC CTCATGGTCT TCAGCATCTG GTTGTATCGC
    CACAGAAAGA AGCGAAATGG ACTCACTAGT ACTTACGCTG GTATCAGAAA AGTCCCGTCT
    TTTACCTTCA CACCAACAGT AACCTATCAG AGAGGAGGAG ATGCCGTAAG CAGTGGAGGC
    AGGCCAGGAC TCCTAAACAT CAGTGAACCA GCCACACAGC CCTGGCTAGC AGATACTTGG
    CCTAACACAG GGAACAACCA CAATGACTGC TCCATCAACT GCTGCACCTC TGGCAATGGA
    AACAGTGATG GCAACCTTAC GACATACAGT CGACCAGCCG ATTGTATAGC AAATTACAAC
    AATCAGCTGG ACAACAAGCA GACAAACCTG ATGTTGCCTG AGTCAACTGT ATATGGTGAC
    GTGGACCTAA GCAACAAAAT CAATGAGATG AAAACCTTCA ATAGTCCAAA TCTAAAGGAT
    GGAAGATTTG TCAGCCAACC TGGGCAGCCC ACTCCTTATG CCACCACTCA ACTCATCCAG
    TCAAATATCA GCAATAATGT TAACAATGGC AGCGGGGATA CGAATGAGAA ACACTGGAAA
    CCCTCTGTTC AGCAAAAGCA AGAGGTTGCA CCCATACAGT ACAACATTAT GGAGCAAAAC
    AAATTAAACA AAGATTACCG AGTAAATGAC AGCATTGCTC CAACAATTCC ATACAACCAG
    TCATATGACC AGAATACAGG TGGATCCTAC AACAGCTCAG ATAGAGGCAG TAGCACATCT
    GGGAGTCAAG GGCACAAGAA GGGTGCTCGG ACTCCAAAGG CTCCCAAGCA AGCTGGCATG
    AACTGGGCAG ATCTTCTTCC ACCCCCTCCA GCACATCCAC CTCCCCATAG CAATAGTGAA
    GATTACAGTC TTTCAGTGGA TGAAAGCTAT GACCAAGAAA TTCCATGTCC AGTCCCACCT
    GCAAGGATGT ATCTGCAGCA AGATGAGCTA GAGGAAGAGG AAGAAGATGA GCGAGGTCCT
    ACTCCACCTG TCCGAGGAGC AGCTTCCTCT CCAGCCGCAG TCTCCTATAG TCATCAGTCA
    ACTGCCACTC TCACCCCTTC TCCCCAGGAA GAACTCCAGC CCATGTTACA GGACTGTCAG
    GAGGATCTGG GACACATACA ACACCAACCT GAACGGAGAC GTCAGCCTGT GAGCCCTCCA
    CCCCCTCCAA GGCCTATCTC TCCACCACAT ACCTATGGAT ATATCTCAGG GCCTTTAGTC
    TCTGACATGG ATACTGATGC ACCAGAAGAG GAAGAAGATG AGGCAGATAT GGAGGTTGCT
    AAGATGCAAA ACAGAAGACT CCTTTTGCGT GGCCTTGAGC AGACACCTGC TTCAAGTGTT
    GGTGATTTGG AGAGCTCTGT AACAGGGTCC ATGATCAATG GCTGGGGTTC AGCTTCCGAA
    GAAGATAACA TCTCAAGTGG GCGGTCTAGT GTTAGCTCTT CAGATGGATC ATTTTTTACT
    GATGCAGATT TTGCACAGGC TGTTGCAGCA GCTGCAGAAT ATGCTGGCTT GAAAGTAGCC
    AGACGTCAAA TGCAGGATGC AGCAGGAGGC CGTAGACATT TTCATGCATC ACATTGTCCC
    AGACCTACCA GCCCTGTATC TACTGACAGC AACATGAGTG CTGTTGTAAT ACAGAAGGTC
    CGACCTGCCA AAAAGCAAAA ACACCAGCCA GGACATCTGC GCAGAGAAGT CTACACAGAT
    GACTTGCCAC CCCCTCCCGT GCCACCTCCC GCTATAAAAT CACCAACCGC CCAGTCCAAA
    TCTCAGCTGG AGGTGCGACC TGTCATGTTG CCAAAACTGG CTTCTATAGA ACCGAGGACA
    GACAGATCTG CGGAAAGAAA AGGAGTGAGC TACAAGGGGA GGGACGGGGT GGATGGGAGA
    CAGCATTCAG ACATGCGGAC AAATTCAGGA GAGCGGAGAG AATCACAGGA ACAGCAAGGC
    GACGGGAAAC TGCGAGGAAG TAAAGCACCA AAACGAGAAG GCACACCAGC AAAAACTCAT
    CTTCTTCAAG AGGACATCCT GCCATATTCT AGACCAACGT TTCCAACGTC AAATAATCCC
    AGAGATCCCA GCTCCTCCAG CTCTATGTCT TCGAGAGGAT CAGGAGGGAG ACAGAGGGCA
    GATCAAGCAA ATATAGCCCG AAGAAATGTT GCAGAAATGC AAGTACTGGG AGCATATGAA
    CAAGGAGAAG AAGAAGAAGA TGAGATGGAG GAAACAGAAA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30203
    Clone ID Related Accession (Same CDS sequence) XM_015298487.2 , XM_015298487.1
    Accession Version XM_015298487.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298487.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTAACCTATC AGAGAGGAGG AGATGCCGTA AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC
    ATCAGTGAAC CAGCCACACA GCCCTGGCTA GCAGATACTT GGCCTAACAC AGGGAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACC TCTGGCAATG GAAACAGTGA TGGCAACCTT
    ACGACATACA GTCGACCAGC CGATTGTATA GCAAATTACA ACAATCAGCT GGACAACAAG
    CAGACAAACC TGATGTTGCC TGAGTCAACT GTATATGGTG ACGTGGACCT AAGCAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGTCCA AATCTAAAGG ATGGAAGATT TGTCAGCCAA
    CCTGGGCAGC CCACTCCTTA TGCCACCACT CAACTCATCC AGTCAAATAT CAGCAATAAT
    GTTAACAATG GCAGCGGGGA TACGAATGAG AAACACTGGA AACCCTCTGT TCAGCAAAAG
    CAAGAGGTTG CACCCATACA GTACAACATT ATGGAGCAAA ACAAATTAAA CAAAGATTAC
    CGAGTAAATG ACAGCATTGC TCCAACAATT CCATACAACC AGTCATATGA CCAGAATACA
    GGTGGATCCT ACAACAGCTC AGATAGAGGC AGTAGCACAT CTGGGAGTCA AGGGCACAAG
    AAGGGTGCTC GGACTCCAAA GGCTCCCAAG CAAGCTGGCA TGAACTGGGC AGATCTTCTT
    CCACCCCCTC CAGCACATCC ACCTCCCCAT AGCAATAGTG AAGATTACAG TCTTTCAGTG
    GATGAAAGCT ATGACCAAGA AATTCCATGT CCAGTCCCAC CTGCAAGGAT GTATCTGCAG
    CAAGATGAGC TAGAGGAAGA GGAAGAAGAT GAGCGAGGTC CTACTCCACC TGTCCGAGGA
    GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT AGTCATCAGT CAACTGCCAC TCTCACCCCT
    TCTCCCCAGG AAGAACTCCA GCCCATGTTA CAGGACTGTC AGGAGGATCT GGGACACATA
    CAACACCAAC CTGAACGGAG ACGTCAGCCT GTGAGCCCTC CACCCCCTCC AAGGCCTATC
    TCTCCACCAC ATACCTATGG ATATATCTCA GGGCCTTTAG TCTCTGACAT GGATACTGAT
    GCACCAGAAG AGGAAGAAGA TGAGGCAGAT ATGGAGGTTG CTAAGATGCA AAACAGAAGA
    CTCCTTTTGC GTGGCCTTGA GCAGACACCT GCTTCAAGTG TTGGTGATTT GGAGAGCTCT
    GTAACAGGGT CCATGATCAA TGGCTGGGGT TCAGCTTCCG AAGAAGATAA CATCTCAAGT
    GGGCGGTCTA GTGTTAGCTC TTCAGATGGA TCATTTTTTA CTGATGCAGA TTTTGCACAG
    GCTGTTGCAG CAGCTGCAGA ATATGCTGGC TTGAAAGTAG CCAGACGTCA AATGCAGGAT
    GCAGCAGGAG GCCGTAGACA TTTTCATGCA TCACATTGTC CCAGACCTAC CAGCCCTGTA
    TCTACTGACA GCAACATGAG TGCTGTTGTA ATACAGAAGG TCCGACCTGC CAAAAAGCAA
    AAACACCAGC CAGGACATCT GCGCAGAGAA GTCTACACAG ATGACTTGCC ACCCCCTCCC
    GTGCCACCTC CCGCTATAAA ATCACCAACC GCCCAGTCCA AATCTCAGCT GGAGGTGCGA
    CCTGTCATGT TGCCAAAACT GGCTTCTATA GAACCGAGGA CAGACAGATC TGCGGAAAGA
    AAAGGAGTGA GCTACAAGGG GAGGGACGGG GTGGATGGGA GACAGCATTC AGACATGCGG
    ACAAATTCAG GAGAGCGGAG AGAATCACAG GAACAGCAAG GCGACGGGAA ACTGCGAGGA
    AGTAAAGCAC CAAAACGAGA AGGCACACCA GCAAAAACTC ATCTTCTTCA AGAGGACATC
    CTGCCATATT CTAGACCAAC GTTTCCAACG TCAAATAATC CCAGAGATCC CAGCTCCTCC
    AGCTCTATGT CTTCGAGAGG ATCAGGAGGG AGACAGAGGG CAGATCAAGC AAATATAGCC
    CGAAGAAATG TTGCAGAAAT GCAAGTACTG GGAGCATATG AACAAGGAGA AGAAGAAGAA
    GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153973.1
    CDS325..5271
    Translation

    Target ORF information:

    RefSeq Version XM_015298487.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298487.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTAACCTATC AGAGAGGAGG AGATGCCGTA AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC
    ATCAGTGAAC CAGCCACACA GCCCTGGCTA GCAGATACTT GGCCTAACAC AGGGAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACC TCTGGCAATG GAAACAGTGA TGGCAACCTT
    ACGACATACA GTCGACCAGC CGATTGTATA GCAAATTACA ACAATCAGCT GGACAACAAG
    CAGACAAACC TGATGTTGCC TGAGTCAACT GTATATGGTG ACGTGGACCT AAGCAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGTCCA AATCTAAAGG ATGGAAGATT TGTCAGCCAA
    CCTGGGCAGC CCACTCCTTA TGCCACCACT CAACTCATCC AGTCAAATAT CAGCAATAAT
    GTTAACAATG GCAGCGGGGA TACGAATGAG AAACACTGGA AACCCTCTGT TCAGCAAAAG
    CAAGAGGTTG CACCCATACA GTACAACATT ATGGAGCAAA ACAAATTAAA CAAAGATTAC
    CGAGTAAATG ACAGCATTGC TCCAACAATT CCATACAACC AGTCATATGA CCAGAATACA
    GGTGGATCCT ACAACAGCTC AGATAGAGGC AGTAGCACAT CTGGGAGTCA AGGGCACAAG
    AAGGGTGCTC GGACTCCAAA GGCTCCCAAG CAAGCTGGCA TGAACTGGGC AGATCTTCTT
    CCACCCCCTC CAGCACATCC ACCTCCCCAT AGCAATAGTG AAGATTACAG TCTTTCAGTG
    GATGAAAGCT ATGACCAAGA AATTCCATGT CCAGTCCCAC CTGCAAGGAT GTATCTGCAG
    CAAGATGAGC TAGAGGAAGA GGAAGAAGAT GAGCGAGGTC CTACTCCACC TGTCCGAGGA
    GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT AGTCATCAGT CAACTGCCAC TCTCACCCCT
    TCTCCCCAGG AAGAACTCCA GCCCATGTTA CAGGACTGTC AGGAGGATCT GGGACACATA
    CAACACCAAC CTGAACGGAG ACGTCAGCCT GTGAGCCCTC CACCCCCTCC AAGGCCTATC
    TCTCCACCAC ATACCTATGG ATATATCTCA GGGCCTTTAG TCTCTGACAT GGATACTGAT
    GCACCAGAAG AGGAAGAAGA TGAGGCAGAT ATGGAGGTTG CTAAGATGCA AAACAGAAGA
    CTCCTTTTGC GTGGCCTTGA GCAGACACCT GCTTCAAGTG TTGGTGATTT GGAGAGCTCT
    GTAACAGGGT CCATGATCAA TGGCTGGGGT TCAGCTTCCG AAGAAGATAA CATCTCAAGT
    GGGCGGTCTA GTGTTAGCTC TTCAGATGGA TCATTTTTTA CTGATGCAGA TTTTGCACAG
    GCTGTTGCAG CAGCTGCAGA ATATGCTGGC TTGAAAGTAG CCAGACGTCA AATGCAGGAT
    GCAGCAGGAG GCCGTAGACA TTTTCATGCA TCACATTGTC CCAGACCTAC CAGCCCTGTA
    TCTACTGACA GCAACATGAG TGCTGTTGTA ATACAGAAGG TCCGACCTGC CAAAAAGCAA
    AAACACCAGC CAGGACATCT GCGCAGAGAA GTCTACACAG ATGACTTGCC ACCCCCTCCC
    GTGCCACCTC CCGCTATAAA ATCACCAACC GCCCAGTCCA AATCTCAGCT GGAGGTGCGA
    CCTGTCATGT TGCCAAAACT GGCTTCTATA GAACCGAGGA CAGACAGATC TGCGGAAAGA
    AAAGGAGTGA GCTACAAGGG GAGGGACGGG GTGGATGGGA GACAGCATTC AGACATGCGG
    ACAAATTCAG GAGAGCGGAG AGAATCACAG GAACAGCAAG GCGACGGGAA ACTGCGAGGA
    AGTAAAGCAC CAAAACGAGA AGGCACACCA GCAAAAACTC ATCTTCTTCA AGAGGACATC
    CTGCCATATT CTAGACCAAC GTTTCCAACG TCAAATAATC CCAGAGATCC CAGCTCCTCC
    AGCTCTATGT CTTCGAGAGG ATCAGGAGGG AGACAGAGGG CAGATCAAGC AAATATAGCC
    CGAAGAAATG TTGCAGAAAT GCAAGTACTG GGAGCATATG AACAAGGAGA AGAAGAAGAA
    GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30203
    Clone ID Related Accession (Same CDS sequence) XM_015298487.2 , XM_015298487.1
    Accession Version XM_015298487.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTAACCTATC AGAGAGGAGG AGATGCCGTA AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC
    ATCAGTGAAC CAGCCACACA GCCCTGGCTA GCAGATACTT GGCCTAACAC AGGGAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACC TCTGGCAATG GAAACAGTGA TGGCAACCTT
    ACGACATACA GTCGACCAGC CGATTGTATA GCAAATTACA ACAATCAGCT GGACAACAAG
    CAGACAAACC TGATGTTGCC TGAGTCAACT GTATATGGTG ACGTGGACCT AAGCAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGTCCA AATCTAAAGG ATGGAAGATT TGTCAGCCAA
    CCTGGGCAGC CCACTCCTTA TGCCACCACT CAACTCATCC AGTCAAATAT CAGCAATAAT
    GTTAACAATG GCAGCGGGGA TACGAATGAG AAACACTGGA AACCCTCTGT TCAGCAAAAG
    CAAGAGGTTG CACCCATACA GTACAACATT ATGGAGCAAA ACAAATTAAA CAAAGATTAC
    CGAGTAAATG ACAGCATTGC TCCAACAATT CCATACAACC AGTCATATGA CCAGAATACA
    GGTGGATCCT ACAACAGCTC AGATAGAGGC AGTAGCACAT CTGGGAGTCA AGGGCACAAG
    AAGGGTGCTC GGACTCCAAA GGCTCCCAAG CAAGCTGGCA TGAACTGGGC AGATCTTCTT
    CCACCCCCTC CAGCACATCC ACCTCCCCAT AGCAATAGTG AAGATTACAG TCTTTCAGTG
    GATGAAAGCT ATGACCAAGA AATTCCATGT CCAGTCCCAC CTGCAAGGAT GTATCTGCAG
    CAAGATGAGC TAGAGGAAGA GGAAGAAGAT GAGCGAGGTC CTACTCCACC TGTCCGAGGA
    GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT AGTCATCAGT CAACTGCCAC TCTCACCCCT
    TCTCCCCAGG AAGAACTCCA GCCCATGTTA CAGGACTGTC AGGAGGATCT GGGACACATA
    CAACACCAAC CTGAACGGAG ACGTCAGCCT GTGAGCCCTC CACCCCCTCC AAGGCCTATC
    TCTCCACCAC ATACCTATGG ATATATCTCA GGGCCTTTAG TCTCTGACAT GGATACTGAT
    GCACCAGAAG AGGAAGAAGA TGAGGCAGAT ATGGAGGTTG CTAAGATGCA AAACAGAAGA
    CTCCTTTTGC GTGGCCTTGA GCAGACACCT GCTTCAAGTG TTGGTGATTT GGAGAGCTCT
    GTAACAGGGT CCATGATCAA TGGCTGGGGT TCAGCTTCCG AAGAAGATAA CATCTCAAGT
    GGGCGGTCTA GTGTTAGCTC TTCAGATGGA TCATTTTTTA CTGATGCAGA TTTTGCACAG
    GCTGTTGCAG CAGCTGCAGA ATATGCTGGC TTGAAAGTAG CCAGACGTCA AATGCAGGAT
    GCAGCAGGAG GCCGTAGACA TTTTCATGCA TCACATTGTC CCAGACCTAC CAGCCCTGTA
    TCTACTGACA GCAACATGAG TGCTGTTGTA ATACAGAAGG TCCGACCTGC CAAAAAGCAA
    AAACACCAGC CAGGACATCT GCGCAGAGAA GTCTACACAG ATGACTTGCC ACCCCCTCCC
    GTGCCACCTC CCGCTATAAA ATCACCAACC GCCCAGTCCA AATCTCAGCT GGAGGTGCGA
    CCTGTCATGT TGCCAAAACT GGCTTCTATA GAACCGAGGA CAGACAGATC TGCGGAAAGA
    AAAGGAGTGA GCTACAAGGG GAGGGACGGG GTGGATGGGA GACAGCATTC AGACATGCGG
    ACAAATTCAG GAGAGCGGAG AGAATCACAG GAACAGCAAG GCGACGGGAA ACTGCGAGGA
    AGTAAAGCAC CAAAACGAGA AGGCACACCA GCAAAAACTC ATCTTCTTCA AGAGGACATC
    CTGCCATATT CTAGACCAAC GTTTCCAACG TCAAATAATC CCAGAGATCC CAGCTCCTCC
    AGCTCTATGT CTTCGAGAGG ATCAGGAGGG AGACAGAGGG CAGATCAAGC AAATATAGCC
    CGAAGAAATG TTGCAGAAAT GCAAGTACTG GGAGCATATG AACAAGGAGA AGAAGAAGAA
    GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153973.1
    CDS323..5269
    Translation

    Target ORF information:

    RefSeq Version XM_015298487.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTAACCTATC AGAGAGGAGG AGATGCCGTA AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC
    ATCAGTGAAC CAGCCACACA GCCCTGGCTA GCAGATACTT GGCCTAACAC AGGGAACAAC
    CACAATGACT GCTCCATCAA CTGCTGCACC TCTGGCAATG GAAACAGTGA TGGCAACCTT
    ACGACATACA GTCGACCAGC CGATTGTATA GCAAATTACA ACAATCAGCT GGACAACAAG
    CAGACAAACC TGATGTTGCC TGAGTCAACT GTATATGGTG ACGTGGACCT AAGCAACAAA
    ATCAATGAGA TGAAAACCTT CAATAGTCCA AATCTAAAGG ATGGAAGATT TGTCAGCCAA
    CCTGGGCAGC CCACTCCTTA TGCCACCACT CAACTCATCC AGTCAAATAT CAGCAATAAT
    GTTAACAATG GCAGCGGGGA TACGAATGAG AAACACTGGA AACCCTCTGT TCAGCAAAAG
    CAAGAGGTTG CACCCATACA GTACAACATT ATGGAGCAAA ACAAATTAAA CAAAGATTAC
    CGAGTAAATG ACAGCATTGC TCCAACAATT CCATACAACC AGTCATATGA CCAGAATACA
    GGTGGATCCT ACAACAGCTC AGATAGAGGC AGTAGCACAT CTGGGAGTCA AGGGCACAAG
    AAGGGTGCTC GGACTCCAAA GGCTCCCAAG CAAGCTGGCA TGAACTGGGC AGATCTTCTT
    CCACCCCCTC CAGCACATCC ACCTCCCCAT AGCAATAGTG AAGATTACAG TCTTTCAGTG
    GATGAAAGCT ATGACCAAGA AATTCCATGT CCAGTCCCAC CTGCAAGGAT GTATCTGCAG
    CAAGATGAGC TAGAGGAAGA GGAAGAAGAT GAGCGAGGTC CTACTCCACC TGTCCGAGGA
    GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT AGTCATCAGT CAACTGCCAC TCTCACCCCT
    TCTCCCCAGG AAGAACTCCA GCCCATGTTA CAGGACTGTC AGGAGGATCT GGGACACATA
    CAACACCAAC CTGAACGGAG ACGTCAGCCT GTGAGCCCTC CACCCCCTCC AAGGCCTATC
    TCTCCACCAC ATACCTATGG ATATATCTCA GGGCCTTTAG TCTCTGACAT GGATACTGAT
    GCACCAGAAG AGGAAGAAGA TGAGGCAGAT ATGGAGGTTG CTAAGATGCA AAACAGAAGA
    CTCCTTTTGC GTGGCCTTGA GCAGACACCT GCTTCAAGTG TTGGTGATTT GGAGAGCTCT
    GTAACAGGGT CCATGATCAA TGGCTGGGGT TCAGCTTCCG AAGAAGATAA CATCTCAAGT
    GGGCGGTCTA GTGTTAGCTC TTCAGATGGA TCATTTTTTA CTGATGCAGA TTTTGCACAG
    GCTGTTGCAG CAGCTGCAGA ATATGCTGGC TTGAAAGTAG CCAGACGTCA AATGCAGGAT
    GCAGCAGGAG GCCGTAGACA TTTTCATGCA TCACATTGTC CCAGACCTAC CAGCCCTGTA
    TCTACTGACA GCAACATGAG TGCTGTTGTA ATACAGAAGG TCCGACCTGC CAAAAAGCAA
    AAACACCAGC CAGGACATCT GCGCAGAGAA GTCTACACAG ATGACTTGCC ACCCCCTCCC
    GTGCCACCTC CCGCTATAAA ATCACCAACC GCCCAGTCCA AATCTCAGCT GGAGGTGCGA
    CCTGTCATGT TGCCAAAACT GGCTTCTATA GAACCGAGGA CAGACAGATC TGCGGAAAGA
    AAAGGAGTGA GCTACAAGGG GAGGGACGGG GTGGATGGGA GACAGCATTC AGACATGCGG
    ACAAATTCAG GAGAGCGGAG AGAATCACAG GAACAGCAAG GCGACGGGAA ACTGCGAGGA
    AGTAAAGCAC CAAAACGAGA AGGCACACCA GCAAAAACTC ATCTTCTTCA AGAGGACATC
    CTGCCATATT CTAGACCAAC GTTTCCAACG TCAAATAATC CCAGAGATCC CAGCTCCTCC
    AGCTCTATGT CTTCGAGAGG ATCAGGAGGG AGACAGAGGG CAGATCAAGC AAATATAGCC
    CGAAGAAATG TTGCAGAAAT GCAAGTACTG GGAGCATATG AACAAGGAGA AGAAGAAGAA
    GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30204
    Clone ID Related Accession (Same CDS sequence) XM_015298492.1 , XM_015298492.2
    Accession Version XM_015298492.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTCACTGCT GCTGCTGCTG TGGCTGGTAG GAGTCACTGC TGCTGTGGAC 
    ATCTCGCCTA GAATGAGAGC GGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGGCAAGAAG ATTTCCCACC TCGCATTGTT GAACACCCAT CTGATTTAAT TGTTTCAAAA
    GGAGAACCAG CAACTTTAAA CTGCAAAGCT GAAGGAAGGC CAACACCAAC CATTGAGTGG
    TATAAAGGTG GAGAAAGAGT GGAAACTGAC AAGGATGATC CTCGTTCTCA TCGGATGCTG
    CTGCCCAGCG GATCTTTATT TTTTTTACGC ATAGTTCATG GACGCAAGAG TAGGCCAGAT
    GAAGGGGTCT ATGTCTGCGT GGCAAGGAAC TACCTTGGGG AAGCTGTAAG TCACAATGCA
    TCACTGGAGG TGGCAATTCT ACGGGATGAC TTCAGACAAA ATCCATCAGA TGTAATGGTA
    GCTGTGGGTG AACCTGCTGT GATGGAATGT CAGCCCCCAA GAGGTCACCC AGAACCCACT
    ATATCATGGA AAAAAGATGG GACTCCTCTA GATGACAAAG ATGAGAGAAT TACAATCAGA
    GGTGGAAAGC TTATGATCAC ATACACAAGA AAGAATGATG CTGGCAAGTA CGTTTGTGTT
    GGAACAAATA TGGTTGGAGA ACGTGAAAGT GAAGTTGCAG AGCTCACTGT TTTAGAAAGA
    CCGTCTTTTG TGAAGAGACC AAGTAATTTG GCTGTAACTG TGGATGACAG TGCTGAGTTT
    AAATGTGAGG CAAGAGGTGA CCCAGTCCCT ACAGTAAGAT GGAGGAAAGA TGATGGGGAA
    TTACCAAAAG CGAGATATGA AATTCGTGAT GATCATACCC TGAAAATCCG GAAAGTTATG
    GCAGGAGACA TGGGCTCATA TACTTGTGTA GCAGAAAATA TGGTTGGAAA AGCTGAAGCA
    TCGGCAACTC TAACAGTGCA AGTTGTGTCT GAGCCTCCAC AGTTTGTTGT AAAGCCTCGT
    GATCAGGTAG CTGCATTGGG AAGAACAGTG ACTTTCCAGT GTGAAGCAAC TGGGAACCCA
    CAGCCAGCCA TCTTTTGGCG GAGAGAGGGA AGTCAGAATC TGCTTTTCTC ATACCAGCCC
    CCCCAGTCAT CCAGTCGATT TTCAGTTTCC CAGACAGGAG ACCTCACCAT CACCAATGTC
    CAGAGATCTG ATGTTGGGTA CTACATCTGC CAGACTTTAA ATGTTGCAGG AAGCATCATA
    ACAAAAGCCT ACTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC TGTCATTCGA
    CAGGGTCCTG TGAATCAGAC TGTAGCTGTA GATGGTACTT TAGTACTAAA CTGTGTGGCC
    ACAGGCACTT TAACACCCAC TATCCTCTGG AAAAAGGATG GGACCCTGAT CTCTACCCAG
    GACTCTCGCA TCAAACAGTT GGAGACAGGA GCATTACAGA TCCGATATGC CAAGCTGGGT
    GATACTGGTC GGTACACCTG CGTTGCTTCA ACCCCAAGTG GCGAAGCTAC ATGGAGTGCC
    TACATAGAGG TCCAAGAATT TGGAGTCCCA GTGCAGCCAC CAAGACCCAC TGACCCAAAC
    TTGATTCCTA GTGCTCCATC AAAACCTGAG GTTACAGATG TCAGCAGAAA TACAGTAACA
    TTGTCATGGC AACCTAATTT GAATTCAGGT GCAACGCCAA CCTCTTACAT AATTGAGGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAA ACTGTAGCTG AAAATGTGAA AACAGAGAGT
    TTTGCAGTTA AAGGGCTAAA ACCTAGTGCA ATTTATCTAT TCCTTGTGAG GGCAGCTAAT
    GCATATGGAC TAAGTGACCC AAGCCAAATA TCTGATCCAG TGAAAACTCA AGATGTTCCA
    CCAACAAGTC AAGGTGTGGA TCACAAGCAG GTGCAGAGAG AACTAGGCGA TGTGGTACTA
    CATCTGCACA ACCCTACTAT CCTTTCTTCC TCCTCAATTG AAGTTCATTG GACAGTGGAC
    CAGCAATCCC AATATATTCA AGGATATAAA ATCCTATACC GGCCAACACC AGCGTCCTAT
    GGAGAGTCAG AATGGTTAAT TTTTGAAGTG AGGACTCCAA CCAAAAACAG TGTTATCATT
    CCAGAGCTGA AGAAAGGTGT AAACTATGAA ATCAAGGCCC GCCCTTTTTT TAATGAGTTT
    CAAGGCGCAG ATAGTGAAGT GAAATTTGCA AAAACCCTAG AAGAAGCGCC CAGTGCTCCA
    CCTCAGAGTG TTTCAGTAAC TAAAAATGAT GGGAATGGAA CTGCAATTGT AGTCACCTGG
    CAGCCACCCC CCGAGGATAA CCAGAACGGA ATGGTTCAAG AATACAAGGT TTGGTGCCTT
    GGCAATGAAA GTCGATACCA TATAAACAGG ACAGTAGATG GTTCCACCTT TTCAGTAGTC
    ATCCCTTCCC TAGTACCAGG TATTCGATAC AGCGTGGAAG TGGCAGCAAG TACTGGAGCA
    GGACCTGGAG TGAAAAGTGA TCCCCAGTTT ATCCAGTTAG ATTCGCATGG AAATCCTGTG
    TCTTCAGAAG ATCAAGTCAG TTTAGCCCAG CAGATCTCTG ACGTAGTAAA ACAGCCTGCC
    TTTATTGCTG GAATTGGTGC TGCTTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGTTG
    TATCGCCACA GAAAGAAGCG AAATGGACTC ACTAGTACTT ACGCTGGTAT CAGAAAAGTC
    CCGTCTTTTA CCTTCACACC AACAGTAACC TATCAGAGAG GAGGAGATGC CGTAAGCAGT
    GGAGGCAGGC CAGGACTCCT AAACATCAGT GAACCAGCCA CACAGCCCTG GCTAGCAGAT
    ACTTGGCCTA ACACAGGGAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACCTCTGGC
    AATGGAAACA GTGATGGCAA CCTTACGACA TACAGTCGAC CAGCCGATTG TATAGCAAAT
    TACAACAATC AGCTGGACAA CAAGCAGACA AACCTGATGT TGCCTGAGTC AACTGTATAT
    GGTGACGTGG ACCTAAGCAA CAAAATCAAT GAGATGAAAA CCTTCAATAG TCCAAATCTA
    AAGGATGGAA GATTTGTCAG CCAACCTGGG CAGCCCACTC CTTATGCCAC CACTCAACTC
    ATCCAGTCAA ATATCAGCAA TAATGTTAAC AATGGCAGCG GGGATACGAA TGAGAAACAC
    TGGAAACCCT CTGTTCAGCA AAAGCAAGAG GTTGCACCCA TACAGTACAA CATTATGGAG
    CAAAACAAAT TAAACAAAGA TTACCGAGTA AATGACAGCA TTGCTCCAAC AATTCCATAC
    AACCAGTCAT ATGACCAGAA TACAGGTGGA TCCTACAACA GCTCAGATAG AGGCAGTAGC
    ACATCTGGGA GTCAAGGGCA CAAGAAGGGT GCTCGGACTC CAAAGGCTCC CAAGCAAGCT
    GGCATGAACT GGGCAGATCT TCTTCCACCC CCTCCAGCAC ATCCACCTCC CCATAGCAAT
    AGTGAAGATT ACAGTCTTTC AGTGGATGAA AGCTATGACC AAGAAATTCC ATGTCCAGTC
    CCACCTGCAA GGATGTATCT GCAGCAAGAT GAGCTAGAGG AAGAGGAAGA AGATGAGCGA
    GGTCCTACTC CACCTGTCCG AGGAGCAGCT TCCTCTCCAG CCGCAGTCTC CTATAGTCAT
    CAGTCAACTG CCACTCTCAC CCCTTCTCCC CAGGAAGAAC TCCAGCCCAT GTTACAGGAC
    TGTCAGGAGG ATCTGGGACA CATACAACAC CAACCTGAAC GGAGACGTCA GCCTGTGAGC
    CCTCCACCCC CTCCAAGGCC TATCTCTCCA CCACATACCT ATGGATATAT CTCAGGGCCT
    TTAGTCTCTG ACATGGATAC TGATGCACCA GAAGAGGAAG AAGATGAGGC AGATATGGAG
    GTTGCTAAGA TGCAAAACAG AAGACTCCTT TTGCGTGGCC TTGAGCAGAC ACCTGCTTCA
    AGTGTTGGTG ATTTGGAGAG CTCTGTAACA GGGTCCATGA TCAATGGCTG GGGTTCAGCT
    TCCGAAGAAG ATAACATCTC AAGTGGGCGG TCTAGTGTTA GCTCTTCAGA TGGATCATTT
    TTTACTGATG CAGATTTTGC ACAGGCTGTT GCAGCAGCTG CAGAATATGC TGGCTTGAAA
    GTAGCCAGAC GTCAAATGCA GGATGCAGCA GGAGGCCGTA GACATTTTCA TGCATCACAT
    TGTCCCAGAC CTACCAGCCC TGTATCTACT GACAGCAACA TGAGTGCTGT TGTAATACAG
    AAGGTCCGAC CTGCCAAAAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGACT TGCCACCCCC TCCCGTGCCA CCTCCCGCTA TAAAATCACC AACCGCCCAG
    TCCAAATCTC AGCTGGAGGT GCGACCTGTC ATGTTGCCAA AACTGGCTTC TATAGAACCG
    AGGACAGACA GATCTGCGGA AAGAAAAGGA GTGAGCTACA AGGGGAGGGA CGGGGTGGAT
    GGGAGACAGC ATTCAGACAT GCGGACAAAT TCAGGAGAGC GGAGAGAATC ACAGGAACAG
    CAAGGCGACG GGAAACTGCG AGGAAGTAAA GCACCAAAAC GAGAAGGCAC ACCAGCAAAA
    ACTCATCTTC TTCAAGAGGA CATCCTGCCA TATTCTAGAC CAACGTTTCC AACGTCAAAT
    AATCCCAGAG ATCCCAGCTC CTCCAGCTCT ATGTCTTCGA GAGGATCAGG AGGGAGACAG
    AGGGCAGATC AAGCAAATAT AGCCCGAAGA AATGTTGCAG AAATGCAAGT ACTGGGAGCA
    TATGAACAAG GAGAAGAAGA AGAAGATGAG ATGGAGGAAA CAGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153978.1
    CDS16..4926
    Translation

    Target ORF information:

    RefSeq Version XM_015298492.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298492.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTCACTGCT GCTGCTGCTG TGGCTGGTAG GAGTCACTGC TGCTGTGGAC 
    ATCTCGCCTA GAATGAGAGC GGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGGCAAGAAG ATTTCCCACC TCGCATTGTT GAACACCCAT CTGATTTAAT TGTTTCAAAA
    GGAGAACCAG CAACTTTAAA CTGCAAAGCT GAAGGAAGGC CAACACCAAC CATTGAGTGG
    TATAAAGGTG GAGAAAGAGT GGAAACTGAC AAGGATGATC CTCGTTCTCA TCGGATGCTG
    CTGCCCAGCG GATCTTTATT TTTTTTACGC ATAGTTCATG GACGCAAGAG TAGGCCAGAT
    GAAGGGGTCT ATGTCTGCGT GGCAAGGAAC TACCTTGGGG AAGCTGTAAG TCACAATGCA
    TCACTGGAGG TGGCAATTCT ACGGGATGAC TTCAGACAAA ATCCATCAGA TGTAATGGTA
    GCTGTGGGTG AACCTGCTGT GATGGAATGT CAGCCCCCAA GAGGTCACCC AGAACCCACT
    ATATCATGGA AAAAAGATGG GACTCCTCTA GATGACAAAG ATGAGAGAAT TACAATCAGA
    GGTGGAAAGC TTATGATCAC ATACACAAGA AAGAATGATG CTGGCAAGTA CGTTTGTGTT
    GGAACAAATA TGGTTGGAGA ACGTGAAAGT GAAGTTGCAG AGCTCACTGT TTTAGAAAGA
    CCGTCTTTTG TGAAGAGACC AAGTAATTTG GCTGTAACTG TGGATGACAG TGCTGAGTTT
    AAATGTGAGG CAAGAGGTGA CCCAGTCCCT ACAGTAAGAT GGAGGAAAGA TGATGGGGAA
    TTACCAAAAG CGAGATATGA AATTCGTGAT GATCATACCC TGAAAATCCG GAAAGTTATG
    GCAGGAGACA TGGGCTCATA TACTTGTGTA GCAGAAAATA TGGTTGGAAA AGCTGAAGCA
    TCGGCAACTC TAACAGTGCA AGTTGTGTCT GAGCCTCCAC AGTTTGTTGT AAAGCCTCGT
    GATCAGGTAG CTGCATTGGG AAGAACAGTG ACTTTCCAGT GTGAAGCAAC TGGGAACCCA
    CAGCCAGCCA TCTTTTGGCG GAGAGAGGGA AGTCAGAATC TGCTTTTCTC ATACCAGCCC
    CCCCAGTCAT CCAGTCGATT TTCAGTTTCC CAGACAGGAG ACCTCACCAT CACCAATGTC
    CAGAGATCTG ATGTTGGGTA CTACATCTGC CAGACTTTAA ATGTTGCAGG AAGCATCATA
    ACAAAAGCCT ACTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC TGTCATTCGA
    CAGGGTCCTG TGAATCAGAC TGTAGCTGTA GATGGTACTT TAGTACTAAA CTGTGTGGCC
    ACAGGCACTT TAACACCCAC TATCCTCTGG AAAAAGGATG GGACCCTGAT CTCTACCCAG
    GACTCTCGCA TCAAACAGTT GGAGACAGGA GCATTACAGA TCCGATATGC CAAGCTGGGT
    GATACTGGTC GGTACACCTG CGTTGCTTCA ACCCCAAGTG GCGAAGCTAC ATGGAGTGCC
    TACATAGAGG TCCAAGAATT TGGAGTCCCA GTGCAGCCAC CAAGACCCAC TGACCCAAAC
    TTGATTCCTA GTGCTCCATC AAAACCTGAG GTTACAGATG TCAGCAGAAA TACAGTAACA
    TTGTCATGGC AACCTAATTT GAATTCAGGT GCAACGCCAA CCTCTTACAT AATTGAGGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAA ACTGTAGCTG AAAATGTGAA AACAGAGAGT
    TTTGCAGTTA AAGGGCTAAA ACCTAGTGCA ATTTATCTAT TCCTTGTGAG GGCAGCTAAT
    GCATATGGAC TAAGTGACCC AAGCCAAATA TCTGATCCAG TGAAAACTCA AGATGTTCCA
    CCAACAAGTC AAGGTGTGGA TCACAAGCAG GTGCAGAGAG AACTAGGCGA TGTGGTACTA
    CATCTGCACA ACCCTACTAT CCTTTCTTCC TCCTCAATTG AAGTTCATTG GACAGTGGAC
    CAGCAATCCC AATATATTCA AGGATATAAA ATCCTATACC GGCCAACACC AGCGTCCTAT
    GGAGAGTCAG AATGGTTAAT TTTTGAAGTG AGGACTCCAA CCAAAAACAG TGTTATCATT
    CCAGAGCTGA AGAAAGGTGT AAACTATGAA ATCAAGGCCC GCCCTTTTTT TAATGAGTTT
    CAAGGCGCAG ATAGTGAAGT GAAATTTGCA AAAACCCTAG AAGAAGCGCC CAGTGCTCCA
    CCTCAGAGTG TTTCAGTAAC TAAAAATGAT GGGAATGGAA CTGCAATTGT AGTCACCTGG
    CAGCCACCCC CCGAGGATAA CCAGAACGGA ATGGTTCAAG AATACAAGGT TTGGTGCCTT
    GGCAATGAAA GTCGATACCA TATAAACAGG ACAGTAGATG GTTCCACCTT TTCAGTAGTC
    ATCCCTTCCC TAGTACCAGG TATTCGATAC AGCGTGGAAG TGGCAGCAAG TACTGGAGCA
    GGACCTGGAG TGAAAAGTGA TCCCCAGTTT ATCCAGTTAG ATTCGCATGG AAATCCTGTG
    TCTTCAGAAG ATCAAGTCAG TTTAGCCCAG CAGATCTCTG ACGTAGTAAA ACAGCCTGCC
    TTTATTGCTG GAATTGGTGC TGCTTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGTTG
    TATCGCCACA GAAAGAAGCG AAATGGACTC ACTAGTACTT ACGCTGGTAT CAGAAAAGTC
    CCGTCTTTTA CCTTCACACC AACAGTAACC TATCAGAGAG GAGGAGATGC CGTAAGCAGT
    GGAGGCAGGC CAGGACTCCT AAACATCAGT GAACCAGCCA CACAGCCCTG GCTAGCAGAT
    ACTTGGCCTA ACACAGGGAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACCTCTGGC
    AATGGAAACA GTGATGGCAA CCTTACGACA TACAGTCGAC CAGCCGATTG TATAGCAAAT
    TACAACAATC AGCTGGACAA CAAGCAGACA AACCTGATGT TGCCTGAGTC AACTGTATAT
    GGTGACGTGG ACCTAAGCAA CAAAATCAAT GAGATGAAAA CCTTCAATAG TCCAAATCTA
    AAGGATGGAA GATTTGTCAG CCAACCTGGG CAGCCCACTC CTTATGCCAC CACTCAACTC
    ATCCAGTCAA ATATCAGCAA TAATGTTAAC AATGGCAGCG GGGATACGAA TGAGAAACAC
    TGGAAACCCT CTGTTCAGCA AAAGCAAGAG GTTGCACCCA TACAGTACAA CATTATGGAG
    CAAAACAAAT TAAACAAAGA TTACCGAGTA AATGACAGCA TTGCTCCAAC AATTCCATAC
    AACCAGTCAT ATGACCAGAA TACAGGTGGA TCCTACAACA GCTCAGATAG AGGCAGTAGC
    ACATCTGGGA GTCAAGGGCA CAAGAAGGGT GCTCGGACTC CAAAGGCTCC CAAGCAAGCT
    GGCATGAACT GGGCAGATCT TCTTCCACCC CCTCCAGCAC ATCCACCTCC CCATAGCAAT
    AGTGAAGATT ACAGTCTTTC AGTGGATGAA AGCTATGACC AAGAAATTCC ATGTCCAGTC
    CCACCTGCAA GGATGTATCT GCAGCAAGAT GAGCTAGAGG AAGAGGAAGA AGATGAGCGA
    GGTCCTACTC CACCTGTCCG AGGAGCAGCT TCCTCTCCAG CCGCAGTCTC CTATAGTCAT
    CAGTCAACTG CCACTCTCAC CCCTTCTCCC CAGGAAGAAC TCCAGCCCAT GTTACAGGAC
    TGTCAGGAGG ATCTGGGACA CATACAACAC CAACCTGAAC GGAGACGTCA GCCTGTGAGC
    CCTCCACCCC CTCCAAGGCC TATCTCTCCA CCACATACCT ATGGATATAT CTCAGGGCCT
    TTAGTCTCTG ACATGGATAC TGATGCACCA GAAGAGGAAG AAGATGAGGC AGATATGGAG
    GTTGCTAAGA TGCAAAACAG AAGACTCCTT TTGCGTGGCC TTGAGCAGAC ACCTGCTTCA
    AGTGTTGGTG ATTTGGAGAG CTCTGTAACA GGGTCCATGA TCAATGGCTG GGGTTCAGCT
    TCCGAAGAAG ATAACATCTC AAGTGGGCGG TCTAGTGTTA GCTCTTCAGA TGGATCATTT
    TTTACTGATG CAGATTTTGC ACAGGCTGTT GCAGCAGCTG CAGAATATGC TGGCTTGAAA
    GTAGCCAGAC GTCAAATGCA GGATGCAGCA GGAGGCCGTA GACATTTTCA TGCATCACAT
    TGTCCCAGAC CTACCAGCCC TGTATCTACT GACAGCAACA TGAGTGCTGT TGTAATACAG
    AAGGTCCGAC CTGCCAAAAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGACT TGCCACCCCC TCCCGTGCCA CCTCCCGCTA TAAAATCACC AACCGCCCAG
    TCCAAATCTC AGCTGGAGGT GCGACCTGTC ATGTTGCCAA AACTGGCTTC TATAGAACCG
    AGGACAGACA GATCTGCGGA AAGAAAAGGA GTGAGCTACA AGGGGAGGGA CGGGGTGGAT
    GGGAGACAGC ATTCAGACAT GCGGACAAAT TCAGGAGAGC GGAGAGAATC ACAGGAACAG
    CAAGGCGACG GGAAACTGCG AGGAAGTAAA GCACCAAAAC GAGAAGGCAC ACCAGCAAAA
    ACTCATCTTC TTCAAGAGGA CATCCTGCCA TATTCTAGAC CAACGTTTCC AACGTCAAAT
    AATCCCAGAG ATCCCAGCTC CTCCAGCTCT ATGTCTTCGA GAGGATCAGG AGGGAGACAG
    AGGGCAGATC AAGCAAATAT AGCCCGAAGA AATGTTGCAG AAATGCAAGT ACTGGGAGCA
    TATGAACAAG GAGAAGAAGA AGAAGATGAG ATGGAGGAAA CAGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30204
    Clone ID Related Accession (Same CDS sequence) XM_015298492.1 , XM_015298492.2
    Accession Version XM_015298492.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4911bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298492.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTCACTGCT GCTGCTGCTG TGGCTGGTAG GAGTCACTGC TGCTGTGGAC 
    ATCTCGCCTA GAATGAGAGC GGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGGCAAGAAG ATTTCCCACC TCGCATTGTT GAACACCCAT CTGATTTAAT TGTTTCAAAA
    GGAGAACCAG CAACTTTAAA CTGCAAAGCT GAAGGAAGGC CAACACCAAC CATTGAGTGG
    TATAAAGGTG GAGAAAGAGT GGAAACTGAC AAGGATGATC CTCGTTCTCA TCGGATGCTG
    CTGCCCAGCG GATCTTTATT TTTTTTACGC ATAGTTCATG GACGCAAGAG TAGGCCAGAT
    GAAGGGGTCT ATGTCTGCGT GGCAAGGAAC TACCTTGGGG AAGCTGTAAG TCACAATGCA
    TCACTGGAGG TGGCAATTCT ACGGGATGAC TTCAGACAAA ATCCATCAGA TGTAATGGTA
    GCTGTGGGTG AACCTGCTGT GATGGAATGT CAGCCCCCAA GAGGTCACCC AGAACCCACT
    ATATCATGGA AAAAAGATGG GACTCCTCTA GATGACAAAG ATGAGAGAAT TACAATCAGA
    GGTGGAAAGC TTATGATCAC ATACACAAGA AAGAATGATG CTGGCAAGTA CGTTTGTGTT
    GGAACAAATA TGGTTGGAGA ACGTGAAAGT GAAGTTGCAG AGCTCACTGT TTTAGAAAGA
    CCGTCTTTTG TGAAGAGACC AAGTAATTTG GCTGTAACTG TGGATGACAG TGCTGAGTTT
    AAATGTGAGG CAAGAGGTGA CCCAGTCCCT ACAGTAAGAT GGAGGAAAGA TGATGGGGAA
    TTACCAAAAG CGAGATATGA AATTCGTGAT GATCATACCC TGAAAATCCG GAAAGTTATG
    GCAGGAGACA TGGGCTCATA TACTTGTGTA GCAGAAAATA TGGTTGGAAA AGCTGAAGCA
    TCGGCAACTC TAACAGTGCA AGTTGTGTCT GAGCCTCCAC AGTTTGTTGT AAAGCCTCGT
    GATCAGGTAG CTGCATTGGG AAGAACAGTG ACTTTCCAGT GTGAAGCAAC TGGGAACCCA
    CAGCCAGCCA TCTTTTGGCG GAGAGAGGGA AGTCAGAATC TGCTTTTCTC ATACCAGCCC
    CCCCAGTCAT CCAGTCGATT TTCAGTTTCC CAGACAGGAG ACCTCACCAT CACCAATGTC
    CAGAGATCTG ATGTTGGGTA CTACATCTGC CAGACTTTAA ATGTTGCAGG AAGCATCATA
    ACAAAAGCCT ACTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC TGTCATTCGA
    CAGGGTCCTG TGAATCAGAC TGTAGCTGTA GATGGTACTT TAGTACTAAA CTGTGTGGCC
    ACAGGCACTT TAACACCCAC TATCCTCTGG AAAAAGGATG GGACCCTGAT CTCTACCCAG
    GACTCTCGCA TCAAACAGTT GGAGACAGGA GCATTACAGA TCCGATATGC CAAGCTGGGT
    GATACTGGTC GGTACACCTG CGTTGCTTCA ACCCCAAGTG GCGAAGCTAC ATGGAGTGCC
    TACATAGAGG TCCAAGAATT TGGAGTCCCA GTGCAGCCAC CAAGACCCAC TGACCCAAAC
    TTGATTCCTA GTGCTCCATC AAAACCTGAG GTTACAGATG TCAGCAGAAA TACAGTAACA
    TTGTCATGGC AACCTAATTT GAATTCAGGT GCAACGCCAA CCTCTTACAT AATTGAGGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAA ACTGTAGCTG AAAATGTGAA AACAGAGAGT
    TTTGCAGTTA AAGGGCTAAA ACCTAGTGCA ATTTATCTAT TCCTTGTGAG GGCAGCTAAT
    GCATATGGAC TAAGTGACCC AAGCCAAATA TCTGATCCAG TGAAAACTCA AGATGTTCCA
    CCAACAAGTC AAGGTGTGGA TCACAAGCAG GTGCAGAGAG AACTAGGCGA TGTGGTACTA
    CATCTGCACA ACCCTACTAT CCTTTCTTCC TCCTCAATTG AAGTTCATTG GACAGTGGAC
    CAGCAATCCC AATATATTCA AGGATATAAA ATCCTATACC GGCCAACACC AGCGTCCTAT
    GGAGAGTCAG AATGGTTAAT TTTTGAAGTG AGGACTCCAA CCAAAAACAG TGTTATCATT
    CCAGAGCTGA AGAAAGGTGT AAACTATGAA ATCAAGGCCC GCCCTTTTTT TAATGAGTTT
    CAAGGCGCAG ATAGTGAAGT GAAATTTGCA AAAACCCTAG AAGAAGCGCC CAGTGCTCCA
    CCTCAGAGTG TTTCAGTAAC TAAAAATGAT GGGAATGGAA CTGCAATTGT AGTCACCTGG
    CAGCCACCCC CCGAGGATAA CCAGAACGGA ATGGTTCAAG AATACAAGGT TTGGTGCCTT
    GGCAATGAAA GTCGATACCA TATAAACAGG ACAGTAGATG GTTCCACCTT TTCAGTAGTC
    ATCCCTTCCC TAGTACCAGG TATTCGATAC AGCGTGGAAG TGGCAGCAAG TACTGGAGCA
    GGACCTGGAG TGAAAAGTGA TCCCCAGTTT ATCCAGTTAG ATTCGCATGG AAATCCTGTG
    TCTTCAGAAG ATCAAGTCAG TTTAGCCCAG CAGATCTCTG ACGTAGTAAA ACAGCCTGCC
    TTTATTGCTG GAATTGGTGC TGCTTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGTTG
    TATCGCCACA GAAAGAAGCG AAATGGACTC ACTAGTACTT ACGCTGGTAT CAGAAAAGTC
    CCGTCTTTTA CCTTCACACC AACAGTAACC TATCAGAGAG GAGGAGATGC CGTAAGCAGT
    GGAGGCAGGC CAGGACTCCT AAACATCAGT GAACCAGCCA CACAGCCCTG GCTAGCAGAT
    ACTTGGCCTA ACACAGGGAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACCTCTGGC
    AATGGAAACA GTGATGGCAA CCTTACGACA TACAGTCGAC CAGCCGATTG TATAGCAAAT
    TACAACAATC AGCTGGACAA CAAGCAGACA AACCTGATGT TGCCTGAGTC AACTGTATAT
    GGTGACGTGG ACCTAAGCAA CAAAATCAAT GAGATGAAAA CCTTCAATAG TCCAAATCTA
    AAGGATGGAA GATTTGTCAG CCAACCTGGG CAGCCCACTC CTTATGCCAC CACTCAACTC
    ATCCAGTCAA ATATCAGCAA TAATGTTAAC AATGGCAGCG GGGATACGAA TGAGAAACAC
    TGGAAACCCT CTGTTCAGCA AAAGCAAGAG GTTGCACCCA TACAGTACAA CATTATGGAG
    CAAAACAAAT TAAACAAAGA TTACCGAGTA AATGACAGCA TTGCTCCAAC AATTCCATAC
    AACCAGTCAT ATGACCAGAA TACAGGTGGA TCCTACAACA GCTCAGATAG AGGCAGTAGC
    ACATCTGGGA GTCAAGGGCA CAAGAAGGGT GCTCGGACTC CAAAGGCTCC CAAGCAAGCT
    GGCATGAACT GGGCAGATCT TCTTCCACCC CCTCCAGCAC ATCCACCTCC CCATAGCAAT
    AGTGAAGATT ACAGTCTTTC AGTGGATGAA AGCTATGACC AAGAAATTCC ATGTCCAGTC
    CCACCTGCAA GGATGTATCT GCAGCAAGAT GAGCTAGAGG AAGAGGAAGA AGATGAGCGA
    GGTCCTACTC CACCTGTCCG AGGAGCAGCT TCCTCTCCAG CCGCAGTCTC CTATAGTCAT
    CAGTCAACTG CCACTCTCAC CCCTTCTCCC CAGGAAGAAC TCCAGCCCAT GTTACAGGAC
    TGTCAGGAGG ATCTGGGACA CATACAACAC CAACCTGAAC GGAGACGTCA GCCTGTGAGC
    CCTCCACCCC CTCCAAGGCC TATCTCTCCA CCACATACCT ATGGATATAT CTCAGGGCCT
    TTAGTCTCTG ACATGGATAC TGATGCACCA GAAGAGGAAG AAGATGAGGC AGATATGGAG
    GTTGCTAAGA TGCAAAACAG AAGACTCCTT TTGCGTGGCC TTGAGCAGAC ACCTGCTTCA
    AGTGTTGGTG ATTTGGAGAG CTCTGTAACA GGGTCCATGA TCAATGGCTG GGGTTCAGCT
    TCCGAAGAAG ATAACATCTC AAGTGGGCGG TCTAGTGTTA GCTCTTCAGA TGGATCATTT
    TTTACTGATG CAGATTTTGC ACAGGCTGTT GCAGCAGCTG CAGAATATGC TGGCTTGAAA
    GTAGCCAGAC GTCAAATGCA GGATGCAGCA GGAGGCCGTA GACATTTTCA TGCATCACAT
    TGTCCCAGAC CTACCAGCCC TGTATCTACT GACAGCAACA TGAGTGCTGT TGTAATACAG
    AAGGTCCGAC CTGCCAAAAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGACT TGCCACCCCC TCCCGTGCCA CCTCCCGCTA TAAAATCACC AACCGCCCAG
    TCCAAATCTC AGCTGGAGGT GCGACCTGTC ATGTTGCCAA AACTGGCTTC TATAGAACCG
    AGGACAGACA GATCTGCGGA AAGAAAAGGA GTGAGCTACA AGGGGAGGGA CGGGGTGGAT
    GGGAGACAGC ATTCAGACAT GCGGACAAAT TCAGGAGAGC GGAGAGAATC ACAGGAACAG
    CAAGGCGACG GGAAACTGCG AGGAAGTAAA GCACCAAAAC GAGAAGGCAC ACCAGCAAAA
    ACTCATCTTC TTCAAGAGGA CATCCTGCCA TATTCTAGAC CAACGTTTCC AACGTCAAAT
    AATCCCAGAG ATCCCAGCTC CTCCAGCTCT ATGTCTTCGA GAGGATCAGG AGGGAGACAG
    AGGGCAGATC AAGCAAATAT AGCCCGAAGA AATGTTGCAG AAATGCAAGT ACTGGGAGCA
    TATGAACAAG GAGAAGAAGA AGAAGATGAG ATGGAGGAAA CAGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153978.1
    CDS16..4926
    Translation

    Target ORF information:

    RefSeq Version XM_015298492.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298492.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGCTGTGC TGTCACTGCT GCTGCTGCTG TGGCTGGTAG GAGTCACTGC TGCTGTGGAC 
    ATCTCGCCTA GAATGAGAGC GGGGTCAAGG TGGAGAGGTG AAAAGAACCG CTCCCGTCTT
    CGGCAAGAAG ATTTCCCACC TCGCATTGTT GAACACCCAT CTGATTTAAT TGTTTCAAAA
    GGAGAACCAG CAACTTTAAA CTGCAAAGCT GAAGGAAGGC CAACACCAAC CATTGAGTGG
    TATAAAGGTG GAGAAAGAGT GGAAACTGAC AAGGATGATC CTCGTTCTCA TCGGATGCTG
    CTGCCCAGCG GATCTTTATT TTTTTTACGC ATAGTTCATG GACGCAAGAG TAGGCCAGAT
    GAAGGGGTCT ATGTCTGCGT GGCAAGGAAC TACCTTGGGG AAGCTGTAAG TCACAATGCA
    TCACTGGAGG TGGCAATTCT ACGGGATGAC TTCAGACAAA ATCCATCAGA TGTAATGGTA
    GCTGTGGGTG AACCTGCTGT GATGGAATGT CAGCCCCCAA GAGGTCACCC AGAACCCACT
    ATATCATGGA AAAAAGATGG GACTCCTCTA GATGACAAAG ATGAGAGAAT TACAATCAGA
    GGTGGAAAGC TTATGATCAC ATACACAAGA AAGAATGATG CTGGCAAGTA CGTTTGTGTT
    GGAACAAATA TGGTTGGAGA ACGTGAAAGT GAAGTTGCAG AGCTCACTGT TTTAGAAAGA
    CCGTCTTTTG TGAAGAGACC AAGTAATTTG GCTGTAACTG TGGATGACAG TGCTGAGTTT
    AAATGTGAGG CAAGAGGTGA CCCAGTCCCT ACAGTAAGAT GGAGGAAAGA TGATGGGGAA
    TTACCAAAAG CGAGATATGA AATTCGTGAT GATCATACCC TGAAAATCCG GAAAGTTATG
    GCAGGAGACA TGGGCTCATA TACTTGTGTA GCAGAAAATA TGGTTGGAAA AGCTGAAGCA
    TCGGCAACTC TAACAGTGCA AGTTGTGTCT GAGCCTCCAC AGTTTGTTGT AAAGCCTCGT
    GATCAGGTAG CTGCATTGGG AAGAACAGTG ACTTTCCAGT GTGAAGCAAC TGGGAACCCA
    CAGCCAGCCA TCTTTTGGCG GAGAGAGGGA AGTCAGAATC TGCTTTTCTC ATACCAGCCC
    CCCCAGTCAT CCAGTCGATT TTCAGTTTCC CAGACAGGAG ACCTCACCAT CACCAATGTC
    CAGAGATCTG ATGTTGGGTA CTACATCTGC CAGACTTTAA ATGTTGCAGG AAGCATCATA
    ACAAAAGCCT ACTTGGAAGT TACTGATGTG ATTGCAGACC GGCCTCCCCC TGTCATTCGA
    CAGGGTCCTG TGAATCAGAC TGTAGCTGTA GATGGTACTT TAGTACTAAA CTGTGTGGCC
    ACAGGCACTT TAACACCCAC TATCCTCTGG AAAAAGGATG GGACCCTGAT CTCTACCCAG
    GACTCTCGCA TCAAACAGTT GGAGACAGGA GCATTACAGA TCCGATATGC CAAGCTGGGT
    GATACTGGTC GGTACACCTG CGTTGCTTCA ACCCCAAGTG GCGAAGCTAC ATGGAGTGCC
    TACATAGAGG TCCAAGAATT TGGAGTCCCA GTGCAGCCAC CAAGACCCAC TGACCCAAAC
    TTGATTCCTA GTGCTCCATC AAAACCTGAG GTTACAGATG TCAGCAGAAA TACAGTAACA
    TTGTCATGGC AACCTAATTT GAATTCAGGT GCAACGCCAA CCTCTTACAT AATTGAGGCC
    TTCAGCCATG CATCTGGTAG CAGCTGGCAA ACTGTAGCTG AAAATGTGAA AACAGAGAGT
    TTTGCAGTTA AAGGGCTAAA ACCTAGTGCA ATTTATCTAT TCCTTGTGAG GGCAGCTAAT
    GCATATGGAC TAAGTGACCC AAGCCAAATA TCTGATCCAG TGAAAACTCA AGATGTTCCA
    CCAACAAGTC AAGGTGTGGA TCACAAGCAG GTGCAGAGAG AACTAGGCGA TGTGGTACTA
    CATCTGCACA ACCCTACTAT CCTTTCTTCC TCCTCAATTG AAGTTCATTG GACAGTGGAC
    CAGCAATCCC AATATATTCA AGGATATAAA ATCCTATACC GGCCAACACC AGCGTCCTAT
    GGAGAGTCAG AATGGTTAAT TTTTGAAGTG AGGACTCCAA CCAAAAACAG TGTTATCATT
    CCAGAGCTGA AGAAAGGTGT AAACTATGAA ATCAAGGCCC GCCCTTTTTT TAATGAGTTT
    CAAGGCGCAG ATAGTGAAGT GAAATTTGCA AAAACCCTAG AAGAAGCGCC CAGTGCTCCA
    CCTCAGAGTG TTTCAGTAAC TAAAAATGAT GGGAATGGAA CTGCAATTGT AGTCACCTGG
    CAGCCACCCC CCGAGGATAA CCAGAACGGA ATGGTTCAAG AATACAAGGT TTGGTGCCTT
    GGCAATGAAA GTCGATACCA TATAAACAGG ACAGTAGATG GTTCCACCTT TTCAGTAGTC
    ATCCCTTCCC TAGTACCAGG TATTCGATAC AGCGTGGAAG TGGCAGCAAG TACTGGAGCA
    GGACCTGGAG TGAAAAGTGA TCCCCAGTTT ATCCAGTTAG ATTCGCATGG AAATCCTGTG
    TCTTCAGAAG ATCAAGTCAG TTTAGCCCAG CAGATCTCTG ACGTAGTAAA ACAGCCTGCC
    TTTATTGCTG GAATTGGTGC TGCTTGTTGG ATTATCCTCA TGGTCTTCAG CATCTGGTTG
    TATCGCCACA GAAAGAAGCG AAATGGACTC ACTAGTACTT ACGCTGGTAT CAGAAAAGTC
    CCGTCTTTTA CCTTCACACC AACAGTAACC TATCAGAGAG GAGGAGATGC CGTAAGCAGT
    GGAGGCAGGC CAGGACTCCT AAACATCAGT GAACCAGCCA CACAGCCCTG GCTAGCAGAT
    ACTTGGCCTA ACACAGGGAA CAACCACAAT GACTGCTCCA TCAACTGCTG CACCTCTGGC
    AATGGAAACA GTGATGGCAA CCTTACGACA TACAGTCGAC CAGCCGATTG TATAGCAAAT
    TACAACAATC AGCTGGACAA CAAGCAGACA AACCTGATGT TGCCTGAGTC AACTGTATAT
    GGTGACGTGG ACCTAAGCAA CAAAATCAAT GAGATGAAAA CCTTCAATAG TCCAAATCTA
    AAGGATGGAA GATTTGTCAG CCAACCTGGG CAGCCCACTC CTTATGCCAC CACTCAACTC
    ATCCAGTCAA ATATCAGCAA TAATGTTAAC AATGGCAGCG GGGATACGAA TGAGAAACAC
    TGGAAACCCT CTGTTCAGCA AAAGCAAGAG GTTGCACCCA TACAGTACAA CATTATGGAG
    CAAAACAAAT TAAACAAAGA TTACCGAGTA AATGACAGCA TTGCTCCAAC AATTCCATAC
    AACCAGTCAT ATGACCAGAA TACAGGTGGA TCCTACAACA GCTCAGATAG AGGCAGTAGC
    ACATCTGGGA GTCAAGGGCA CAAGAAGGGT GCTCGGACTC CAAAGGCTCC CAAGCAAGCT
    GGCATGAACT GGGCAGATCT TCTTCCACCC CCTCCAGCAC ATCCACCTCC CCATAGCAAT
    AGTGAAGATT ACAGTCTTTC AGTGGATGAA AGCTATGACC AAGAAATTCC ATGTCCAGTC
    CCACCTGCAA GGATGTATCT GCAGCAAGAT GAGCTAGAGG AAGAGGAAGA AGATGAGCGA
    GGTCCTACTC CACCTGTCCG AGGAGCAGCT TCCTCTCCAG CCGCAGTCTC CTATAGTCAT
    CAGTCAACTG CCACTCTCAC CCCTTCTCCC CAGGAAGAAC TCCAGCCCAT GTTACAGGAC
    TGTCAGGAGG ATCTGGGACA CATACAACAC CAACCTGAAC GGAGACGTCA GCCTGTGAGC
    CCTCCACCCC CTCCAAGGCC TATCTCTCCA CCACATACCT ATGGATATAT CTCAGGGCCT
    TTAGTCTCTG ACATGGATAC TGATGCACCA GAAGAGGAAG AAGATGAGGC AGATATGGAG
    GTTGCTAAGA TGCAAAACAG AAGACTCCTT TTGCGTGGCC TTGAGCAGAC ACCTGCTTCA
    AGTGTTGGTG ATTTGGAGAG CTCTGTAACA GGGTCCATGA TCAATGGCTG GGGTTCAGCT
    TCCGAAGAAG ATAACATCTC AAGTGGGCGG TCTAGTGTTA GCTCTTCAGA TGGATCATTT
    TTTACTGATG CAGATTTTGC ACAGGCTGTT GCAGCAGCTG CAGAATATGC TGGCTTGAAA
    GTAGCCAGAC GTCAAATGCA GGATGCAGCA GGAGGCCGTA GACATTTTCA TGCATCACAT
    TGTCCCAGAC CTACCAGCCC TGTATCTACT GACAGCAACA TGAGTGCTGT TGTAATACAG
    AAGGTCCGAC CTGCCAAAAA GCAAAAACAC CAGCCAGGAC ATCTGCGCAG AGAAGTCTAC
    ACAGATGACT TGCCACCCCC TCCCGTGCCA CCTCCCGCTA TAAAATCACC AACCGCCCAG
    TCCAAATCTC AGCTGGAGGT GCGACCTGTC ATGTTGCCAA AACTGGCTTC TATAGAACCG
    AGGACAGACA GATCTGCGGA AAGAAAAGGA GTGAGCTACA AGGGGAGGGA CGGGGTGGAT
    GGGAGACAGC ATTCAGACAT GCGGACAAAT TCAGGAGAGC GGAGAGAATC ACAGGAACAG
    CAAGGCGACG GGAAACTGCG AGGAAGTAAA GCACCAAAAC GAGAAGGCAC ACCAGCAAAA
    ACTCATCTTC TTCAAGAGGA CATCCTGCCA TATTCTAGAC CAACGTTTCC AACGTCAAAT
    AATCCCAGAG ATCCCAGCTC CTCCAGCTCT ATGTCTTCGA GAGGATCAGG AGGGAGACAG
    AGGGCAGATC AAGCAAATAT AGCCCGAAGA AATGTTGCAG AAATGCAAGT ACTGGGAGCA
    TATGAACAAG GAGAAGAAGA AGAAGATGAG ATGGAGGAAA CAGAAAGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30205
    Clone ID Related Accession (Same CDS sequence) XM_416673.5 , XM_416673.6
    Accession Version XM_416673.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_416673.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTTGCGG AGTTTGCTCA CTTTTACCTC TGTGGATTAC TCTGCCTCGC CTCAGGCTCC 
    CGTCTTCGGC AAGAAGATTT CCCACCTCGC ATTGTTGAAC ACCCATCTGA TTTAATTGTT
    TCAAAAGGAG AACCAGCAAC TTTAAACTGC AAAGCTGAAG GAAGGCCAAC ACCAACCATT
    GAGTGGTATA AAGGTGGAGA AAGAGTGGAA ACTGACAAGG ATGATCCTCG TTCTCATCGG
    ATGCTGCTGC CCAGCGGATC TTTATTTTTT TTACGCATAG TTCATGGACG CAAGAGTAGG
    CCAGATGAAG GGGTCTATGT CTGCGTGGCA AGGAACTACC TTGGGGAAGC TGTAAGTCAC
    AATGCATCAC TGGAGGTGGC AATTCTACGG GATGACTTCA GACAAAATCC ATCAGATGTA
    ATGGTAGCTG TGGGTGAACC TGCTGTGATG GAATGTCAGC CCCCAAGAGG TCACCCAGAA
    CCCACTATAT CATGGAAAAA AGATGGGACT CCTCTAGATG ACAAAGATGA GAGAATTACA
    ATCAGAGGTG GAAAGCTTAT GATCACATAC ACAAGAAAGA ATGATGCTGG CAAGTACGTT
    TGTGTTGGAA CAAATATGGT TGGAGAACGT GAAAGTGAAG TTGCAGAGCT CACTGTTTTA
    GAAAGACCGT CTTTTGTGAA GAGACCAAGT AATTTGGCTG TAACTGTGGA TGACAGTGCT
    GAGTTTAAAT GTGAGGCAAG AGGTGACCCA GTCCCTACAG TAAGATGGAG GAAAGATGAT
    GGGGAATTAC CAAAAGCGAG ATATGAAATT CGTGATGATC ATACCCTGAA AATCCGGAAA
    GTTATGGCAG GAGACATGGG CTCATATACT TGTGTAGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCGG CAACTCTAAC AGTGCAAGTT GTGTCTGAGC CTCCACAGTT TGTTGTAAAG
    CCTCGTGATC AGGTAGCTGC ATTGGGAAGA ACAGTGACTT TCCAGTGTGA AGCAACTGGG
    AACCCACAGC CAGCCATCTT TTGGCGGAGA GAGGGAAGTC AGAATCTGCT TTTCTCATAC
    CAGCCCCCCC AGTCATCCAG TCGATTTTCA GTTTCCCAGA CAGGAGACCT CACCATCACC
    AATGTCCAGA GATCTGATGT TGGGTACTAC ATCTGCCAGA CTTTAAATGT TGCAGGAAGC
    ATCATAACAA AAGCCTACTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCTGTC
    ATTCGACAGG GTCCTGTGAA TCAGACTGTA GCTGTAGATG GTACTTTAGT ACTAAACTGT
    GTGGCCACAG GCACTTTAAC ACCCACTATC CTCTGGAAAA AGGATGGGAC CCTGATCTCT
    ACCCAGGACT CTCGCATCAA ACAGTTGGAG ACAGGAGCAT TACAGATCCG ATATGCCAAG
    CTGGGTGATA CTGGTCGGTA CACCTGCGTT GCTTCAACCC CAAGTGGCGA AGCTACATGG
    AGTGCCTACA TAGAGGTCCA AGAATTTGGA GTCCCAGTGC AGCCACCAAG ACCCACTGAC
    CCAAACTTGA TTCCTAGTGC TCCATCAAAA CCTGAGGTTA CAGATGTCAG CAGAAATACA
    GTAACATTGT CATGGCAACC TAATTTGAAT TCAGGTGCAA CGCCAACCTC TTACATAATT
    GAGGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAAACTG TAGCTGAAAA TGTGAAAACA
    GAGAGTTTTG CAGTTAAAGG GCTAAAACCT AGTGCAATTT ATCTATTCCT TGTGAGGGCA
    GCTAATGCAT ATGGACTAAG TGACCCAAGC CAAATATCTG ATCCAGTGAA AACTCAAGAT
    GTTCCACCAA CAAGTCAAGG TGTGGATCAC AAGCAGGTGC AGAGAGAACT AGGCGATGTG
    GTACTACATC TGCACAACCC TACTATCCTT TCTTCCTCCT CAATTGAAGT TCATTGGACA
    GTGGACCAGC AATCCCAATA TATTCAAGGA TATAAAATCC TATACCGGCC AACACCAGCG
    TCCTATGGAG AGTCAGAATG GTTAATTTTT GAAGTGAGGA CTCCAACCAA AAACAGTGTT
    ATCATTCCAG AGCTGAAGAA AGGTGTAAAC TATGAAATCA AGGCCCGCCC TTTTTTTAAT
    GAGTTTCAAG GCGCAGATAG TGAAGTGAAA TTTGCAAAAA CCCTAGAAGA AGCGCCCAGT
    GCTCCACCTC AGAGTGTTTC AGTAACTAAA AATGATGGGA ATGGAACTGC AATTGTAGTC
    ACCTGGCAGC CACCCCCCGA GGATAACCAG AACGGAATGG TTCAAGAATA CAAGGTTTGG
    TGCCTTGGCA ATGAAAGTCG ATACCATATA AACAGGACAG TAGATGGTTC CACCTTTTCA
    GTAGTCATCC CTTCCCTAGT ACCAGGTATT CGATACAGCG TGGAAGTGGC AGCAAGTACT
    GGAGCAGGAC CTGGAGTGAA AAGTGATCCC CAGTTTATCC AGTTAGATTC GCATGGAAAT
    CCTGTGTCTT CAGAAGATCA AGTCAGTTTA GCCCAGCAGA TCTCTGACGT AGTAAAACAG
    CCTGCCTTTA TTGCTGGAAT TGGTGCTGCT TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGTTGTATC GCCACAGAAA GAAGCGAAAT GGACTCACTA GTACTTACGC TGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACCTATC AGAGAGGAGG AGATGCCGTA
    AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC ATCAGTGAAC CAGCCACACA GCCCTGGCTA
    GCAGATACTT GGCCTAACAC AGGGAACAAC CACAATGACT GCTCCATCAA CTGCTGCACC
    TCTGGCAATG GAAACAGTGA TGGCAACCTT ACGACATACA GTCGACCAGC CGATTGTATA
    GCAAATTACA ACAATCAGCT GGACAACAAG CAGACAAACC TGATGTTGCC TGAGTCAACT
    GTATATGGTG ACGTGGACCT AAGCAACAAA ATCAATGAGA TGAAAACCTT CAATAGTCCA
    AATCTAAAGG ATGGAAGATT TGTCAGCCAA CCTGGGCAGC CCACTCCTTA TGCCACCACT
    CAACTCATCC AGTCAAATAT CAGCAATAAT GTTAACAATG GCAGCGGGGA TACGAATGAG
    AAACACTGGA AACCCTCTGT TCAGCAAAAG CAAGAGGTTG CACCCATACA GTACAACATT
    ATGGAGCAAA ACAAATTAAA CAAAGATTAC CGAGTAAATG ACAGCATTGC TCCAACAATT
    CCATACAACC AGTCATATGA CCAGAATACA GGTGGATCCT ACAACAGCTC AGATAGAGGC
    AGTAGCACAT CTGGGAGTCA AGGGCACAAG AAGGGTGCTC GGACTCCAAA GGCTCCCAAG
    CAAGCTGGCA TGAACTGGGC AGATCTTCTT CCACCCCCTC CAGCACATCC ACCTCCCCAT
    AGCAATAGTG AAGATTACAG TCTTTCAGTG GATGAAAGCT ATGACCAAGA AATTCCATGT
    CCAGTCCCAC CTGCAAGGAT GTATCTGCAG CAAGATGAGC TAGAGGAAGA GGAAGAAGAT
    GAGCGAGGTC CTACTCCACC TGTCCGAGGA GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT
    AGTCATCAGT CAACTGCCAC TCTCACCCCT TCTCCCCAGG AAGAACTCCA GCCCATGTTA
    CAGGACTGTC AGGAGGATCT GGGACACATA CAACACCAAC CTGAACGGAG ACGTCAGCCT
    GTGAGCCCTC CACCCCCTCC AAGGCCTATC TCTCCACCAC ATACCTATGG ATATATCTCA
    GGGCCTTTAG TCTCTGACAT GGATACTGAT GCACCAGAAG AGGAAGAAGA TGAGGCAGAT
    ATGGAGGTTG CTAAGATGCA AAACAGAAGA CTCCTTTTGC GTGGCCTTGA GCAGACACCT
    GCTTCAAGTG TTGGTGATTT GGAGAGCTCT GTAACAGGGT CCATGATCAA TGGCTGGGGT
    TCAGCTTCCG AAGAAGATAA CATCTCAAGT GGGCGGTCTA GTGTTAGCTC TTCAGATGGA
    TCATTTTTTA CTGATGCAGA TTTTGCACAG GCTGTTGCAG CAGCTGCAGA ATATGCTGGC
    TTGAAAGTAG CCAGACGTCA AATGCAGGAT GCAGCAGGAG GCCGTAGACA TTTTCATGCA
    TCACATTGTC CCAGACCTAC CAGCCCTGTA TCTACTGACA GCAACATGAG TGCTGTTGTA
    ATACAGAAGG TCCGACCTGC CAAAAAGCAA AAACACCAGC CAGGACATCT GCGCAGAGAA
    GTCTACACAG ATGACTTGCC ACCCCCTCCC GTGCCACCTC CCGCTATAAA ATCACCAACC
    GCCCAGTCCA AATCTCAGCT GGAGGTGCGA CCTGTCATGT TGCCAAAACT GGCTTCTATA
    GAACCGAGGA CAGACAGATC TGCGGAAAGA AAAGGAGTGA GCTACAAGGG GAGGGACGGG
    GTGGATGGGA GACAGCATTC AGACATGCGG ACAAATTCAG GAGAGCGGAG AGAATCACAG
    GAACAGCAAG GCGACGGGAA ACTGCGAGGA AGTAAAGCAC CAAAACGAGA AGGCACACCA
    GCAAAAACTC ATCTTCTTCA AGAGGACATC CTGCCATATT CTAGACCAAC GTTTCCAACG
    TCAAATAATC CCAGAGATCC CAGCTCCTCC AGCTCTATGT CTTCGAGAGG ATCAGGAGGG
    AGACAGAGGG CAGATCAAGC AAATATAGCC CGAAGAAATG TTGCAGAAAT GCAAGTACTG
    GGAGCATATG AACAAGGAGA AGAAGAAGAA GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_416673.5
    CDS814..5670
    Translation

    Target ORF information:

    RefSeq Version XM_416673.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_416673.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTTGCGG AGTTTGCTCA CTTTTACCTC TGTGGATTAC TCTGCCTCGC CTCAGGCTCC 
    CGTCTTCGGC AAGAAGATTT CCCACCTCGC ATTGTTGAAC ACCCATCTGA TTTAATTGTT
    TCAAAAGGAG AACCAGCAAC TTTAAACTGC AAAGCTGAAG GAAGGCCAAC ACCAACCATT
    GAGTGGTATA AAGGTGGAGA AAGAGTGGAA ACTGACAAGG ATGATCCTCG TTCTCATCGG
    ATGCTGCTGC CCAGCGGATC TTTATTTTTT TTACGCATAG TTCATGGACG CAAGAGTAGG
    CCAGATGAAG GGGTCTATGT CTGCGTGGCA AGGAACTACC TTGGGGAAGC TGTAAGTCAC
    AATGCATCAC TGGAGGTGGC AATTCTACGG GATGACTTCA GACAAAATCC ATCAGATGTA
    ATGGTAGCTG TGGGTGAACC TGCTGTGATG GAATGTCAGC CCCCAAGAGG TCACCCAGAA
    CCCACTATAT CATGGAAAAA AGATGGGACT CCTCTAGATG ACAAAGATGA GAGAATTACA
    ATCAGAGGTG GAAAGCTTAT GATCACATAC ACAAGAAAGA ATGATGCTGG CAAGTACGTT
    TGTGTTGGAA CAAATATGGT TGGAGAACGT GAAAGTGAAG TTGCAGAGCT CACTGTTTTA
    GAAAGACCGT CTTTTGTGAA GAGACCAAGT AATTTGGCTG TAACTGTGGA TGACAGTGCT
    GAGTTTAAAT GTGAGGCAAG AGGTGACCCA GTCCCTACAG TAAGATGGAG GAAAGATGAT
    GGGGAATTAC CAAAAGCGAG ATATGAAATT CGTGATGATC ATACCCTGAA AATCCGGAAA
    GTTATGGCAG GAGACATGGG CTCATATACT TGTGTAGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCGG CAACTCTAAC AGTGCAAGTT GTGTCTGAGC CTCCACAGTT TGTTGTAAAG
    CCTCGTGATC AGGTAGCTGC ATTGGGAAGA ACAGTGACTT TCCAGTGTGA AGCAACTGGG
    AACCCACAGC CAGCCATCTT TTGGCGGAGA GAGGGAAGTC AGAATCTGCT TTTCTCATAC
    CAGCCCCCCC AGTCATCCAG TCGATTTTCA GTTTCCCAGA CAGGAGACCT CACCATCACC
    AATGTCCAGA GATCTGATGT TGGGTACTAC ATCTGCCAGA CTTTAAATGT TGCAGGAAGC
    ATCATAACAA AAGCCTACTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCTGTC
    ATTCGACAGG GTCCTGTGAA TCAGACTGTA GCTGTAGATG GTACTTTAGT ACTAAACTGT
    GTGGCCACAG GCACTTTAAC ACCCACTATC CTCTGGAAAA AGGATGGGAC CCTGATCTCT
    ACCCAGGACT CTCGCATCAA ACAGTTGGAG ACAGGAGCAT TACAGATCCG ATATGCCAAG
    CTGGGTGATA CTGGTCGGTA CACCTGCGTT GCTTCAACCC CAAGTGGCGA AGCTACATGG
    AGTGCCTACA TAGAGGTCCA AGAATTTGGA GTCCCAGTGC AGCCACCAAG ACCCACTGAC
    CCAAACTTGA TTCCTAGTGC TCCATCAAAA CCTGAGGTTA CAGATGTCAG CAGAAATACA
    GTAACATTGT CATGGCAACC TAATTTGAAT TCAGGTGCAA CGCCAACCTC TTACATAATT
    GAGGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAAACTG TAGCTGAAAA TGTGAAAACA
    GAGAGTTTTG CAGTTAAAGG GCTAAAACCT AGTGCAATTT ATCTATTCCT TGTGAGGGCA
    GCTAATGCAT ATGGACTAAG TGACCCAAGC CAAATATCTG ATCCAGTGAA AACTCAAGAT
    GTTCCACCAA CAAGTCAAGG TGTGGATCAC AAGCAGGTGC AGAGAGAACT AGGCGATGTG
    GTACTACATC TGCACAACCC TACTATCCTT TCTTCCTCCT CAATTGAAGT TCATTGGACA
    GTGGACCAGC AATCCCAATA TATTCAAGGA TATAAAATCC TATACCGGCC AACACCAGCG
    TCCTATGGAG AGTCAGAATG GTTAATTTTT GAAGTGAGGA CTCCAACCAA AAACAGTGTT
    ATCATTCCAG AGCTGAAGAA AGGTGTAAAC TATGAAATCA AGGCCCGCCC TTTTTTTAAT
    GAGTTTCAAG GCGCAGATAG TGAAGTGAAA TTTGCAAAAA CCCTAGAAGA AGCGCCCAGT
    GCTCCACCTC AGAGTGTTTC AGTAACTAAA AATGATGGGA ATGGAACTGC AATTGTAGTC
    ACCTGGCAGC CACCCCCCGA GGATAACCAG AACGGAATGG TTCAAGAATA CAAGGTTTGG
    TGCCTTGGCA ATGAAAGTCG ATACCATATA AACAGGACAG TAGATGGTTC CACCTTTTCA
    GTAGTCATCC CTTCCCTAGT ACCAGGTATT CGATACAGCG TGGAAGTGGC AGCAAGTACT
    GGAGCAGGAC CTGGAGTGAA AAGTGATCCC CAGTTTATCC AGTTAGATTC GCATGGAAAT
    CCTGTGTCTT CAGAAGATCA AGTCAGTTTA GCCCAGCAGA TCTCTGACGT AGTAAAACAG
    CCTGCCTTTA TTGCTGGAAT TGGTGCTGCT TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGTTGTATC GCCACAGAAA GAAGCGAAAT GGACTCACTA GTACTTACGC TGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACCTATC AGAGAGGAGG AGATGCCGTA
    AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC ATCAGTGAAC CAGCCACACA GCCCTGGCTA
    GCAGATACTT GGCCTAACAC AGGGAACAAC CACAATGACT GCTCCATCAA CTGCTGCACC
    TCTGGCAATG GAAACAGTGA TGGCAACCTT ACGACATACA GTCGACCAGC CGATTGTATA
    GCAAATTACA ACAATCAGCT GGACAACAAG CAGACAAACC TGATGTTGCC TGAGTCAACT
    GTATATGGTG ACGTGGACCT AAGCAACAAA ATCAATGAGA TGAAAACCTT CAATAGTCCA
    AATCTAAAGG ATGGAAGATT TGTCAGCCAA CCTGGGCAGC CCACTCCTTA TGCCACCACT
    CAACTCATCC AGTCAAATAT CAGCAATAAT GTTAACAATG GCAGCGGGGA TACGAATGAG
    AAACACTGGA AACCCTCTGT TCAGCAAAAG CAAGAGGTTG CACCCATACA GTACAACATT
    ATGGAGCAAA ACAAATTAAA CAAAGATTAC CGAGTAAATG ACAGCATTGC TCCAACAATT
    CCATACAACC AGTCATATGA CCAGAATACA GGTGGATCCT ACAACAGCTC AGATAGAGGC
    AGTAGCACAT CTGGGAGTCA AGGGCACAAG AAGGGTGCTC GGACTCCAAA GGCTCCCAAG
    CAAGCTGGCA TGAACTGGGC AGATCTTCTT CCACCCCCTC CAGCACATCC ACCTCCCCAT
    AGCAATAGTG AAGATTACAG TCTTTCAGTG GATGAAAGCT ATGACCAAGA AATTCCATGT
    CCAGTCCCAC CTGCAAGGAT GTATCTGCAG CAAGATGAGC TAGAGGAAGA GGAAGAAGAT
    GAGCGAGGTC CTACTCCACC TGTCCGAGGA GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT
    AGTCATCAGT CAACTGCCAC TCTCACCCCT TCTCCCCAGG AAGAACTCCA GCCCATGTTA
    CAGGACTGTC AGGAGGATCT GGGACACATA CAACACCAAC CTGAACGGAG ACGTCAGCCT
    GTGAGCCCTC CACCCCCTCC AAGGCCTATC TCTCCACCAC ATACCTATGG ATATATCTCA
    GGGCCTTTAG TCTCTGACAT GGATACTGAT GCACCAGAAG AGGAAGAAGA TGAGGCAGAT
    ATGGAGGTTG CTAAGATGCA AAACAGAAGA CTCCTTTTGC GTGGCCTTGA GCAGACACCT
    GCTTCAAGTG TTGGTGATTT GGAGAGCTCT GTAACAGGGT CCATGATCAA TGGCTGGGGT
    TCAGCTTCCG AAGAAGATAA CATCTCAAGT GGGCGGTCTA GTGTTAGCTC TTCAGATGGA
    TCATTTTTTA CTGATGCAGA TTTTGCACAG GCTGTTGCAG CAGCTGCAGA ATATGCTGGC
    TTGAAAGTAG CCAGACGTCA AATGCAGGAT GCAGCAGGAG GCCGTAGACA TTTTCATGCA
    TCACATTGTC CCAGACCTAC CAGCCCTGTA TCTACTGACA GCAACATGAG TGCTGTTGTA
    ATACAGAAGG TCCGACCTGC CAAAAAGCAA AAACACCAGC CAGGACATCT GCGCAGAGAA
    GTCTACACAG ATGACTTGCC ACCCCCTCCC GTGCCACCTC CCGCTATAAA ATCACCAACC
    GCCCAGTCCA AATCTCAGCT GGAGGTGCGA CCTGTCATGT TGCCAAAACT GGCTTCTATA
    GAACCGAGGA CAGACAGATC TGCGGAAAGA AAAGGAGTGA GCTACAAGGG GAGGGACGGG
    GTGGATGGGA GACAGCATTC AGACATGCGG ACAAATTCAG GAGAGCGGAG AGAATCACAG
    GAACAGCAAG GCGACGGGAA ACTGCGAGGA AGTAAAGCAC CAAAACGAGA AGGCACACCA
    GCAAAAACTC ATCTTCTTCA AGAGGACATC CTGCCATATT CTAGACCAAC GTTTCCAACG
    TCAAATAATC CCAGAGATCC CAGCTCCTCC AGCTCTATGT CTTCGAGAGG ATCAGGAGGG
    AGACAGAGGG CAGATCAAGC AAATATAGCC CGAAGAAATG TTGCAGAAAT GCAAGTACTG
    GGAGCATATG AACAAGGAGA AGAAGAAGAA GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30205
    Clone ID Related Accession (Same CDS sequence) XM_416673.5 , XM_416673.6
    Accession Version XM_416673.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_416673.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTTGCGG AGTTTGCTCA CTTTTACCTC TGTGGATTAC TCTGCCTCGC CTCAGGCTCC 
    CGTCTTCGGC AAGAAGATTT CCCACCTCGC ATTGTTGAAC ACCCATCTGA TTTAATTGTT
    TCAAAAGGAG AACCAGCAAC TTTAAACTGC AAAGCTGAAG GAAGGCCAAC ACCAACCATT
    GAGTGGTATA AAGGTGGAGA AAGAGTGGAA ACTGACAAGG ATGATCCTCG TTCTCATCGG
    ATGCTGCTGC CCAGCGGATC TTTATTTTTT TTACGCATAG TTCATGGACG CAAGAGTAGG
    CCAGATGAAG GGGTCTATGT CTGCGTGGCA AGGAACTACC TTGGGGAAGC TGTAAGTCAC
    AATGCATCAC TGGAGGTGGC AATTCTACGG GATGACTTCA GACAAAATCC ATCAGATGTA
    ATGGTAGCTG TGGGTGAACC TGCTGTGATG GAATGTCAGC CCCCAAGAGG TCACCCAGAA
    CCCACTATAT CATGGAAAAA AGATGGGACT CCTCTAGATG ACAAAGATGA GAGAATTACA
    ATCAGAGGTG GAAAGCTTAT GATCACATAC ACAAGAAAGA ATGATGCTGG CAAGTACGTT
    TGTGTTGGAA CAAATATGGT TGGAGAACGT GAAAGTGAAG TTGCAGAGCT CACTGTTTTA
    GAAAGACCGT CTTTTGTGAA GAGACCAAGT AATTTGGCTG TAACTGTGGA TGACAGTGCT
    GAGTTTAAAT GTGAGGCAAG AGGTGACCCA GTCCCTACAG TAAGATGGAG GAAAGATGAT
    GGGGAATTAC CAAAAGCGAG ATATGAAATT CGTGATGATC ATACCCTGAA AATCCGGAAA
    GTTATGGCAG GAGACATGGG CTCATATACT TGTGTAGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCGG CAACTCTAAC AGTGCAAGTT GTGTCTGAGC CTCCACAGTT TGTTGTAAAG
    CCTCGTGATC AGGTAGCTGC ATTGGGAAGA ACAGTGACTT TCCAGTGTGA AGCAACTGGG
    AACCCACAGC CAGCCATCTT TTGGCGGAGA GAGGGAAGTC AGAATCTGCT TTTCTCATAC
    CAGCCCCCCC AGTCATCCAG TCGATTTTCA GTTTCCCAGA CAGGAGACCT CACCATCACC
    AATGTCCAGA GATCTGATGT TGGGTACTAC ATCTGCCAGA CTTTAAATGT TGCAGGAAGC
    ATCATAACAA AAGCCTACTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCTGTC
    ATTCGACAGG GTCCTGTGAA TCAGACTGTA GCTGTAGATG GTACTTTAGT ACTAAACTGT
    GTGGCCACAG GCACTTTAAC ACCCACTATC CTCTGGAAAA AGGATGGGAC CCTGATCTCT
    ACCCAGGACT CTCGCATCAA ACAGTTGGAG ACAGGAGCAT TACAGATCCG ATATGCCAAG
    CTGGGTGATA CTGGTCGGTA CACCTGCGTT GCTTCAACCC CAAGTGGCGA AGCTACATGG
    AGTGCCTACA TAGAGGTCCA AGAATTTGGA GTCCCAGTGC AGCCACCAAG ACCCACTGAC
    CCAAACTTGA TTCCTAGTGC TCCATCAAAA CCTGAGGTTA CAGATGTCAG CAGAAATACA
    GTAACATTGT CATGGCAACC TAATTTGAAT TCAGGTGCAA CGCCAACCTC TTACATAATT
    GAGGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAAACTG TAGCTGAAAA TGTGAAAACA
    GAGAGTTTTG CAGTTAAAGG GCTAAAACCT AGTGCAATTT ATCTATTCCT TGTGAGGGCA
    GCTAATGCAT ATGGACTAAG TGACCCAAGC CAAATATCTG ATCCAGTGAA AACTCAAGAT
    GTTCCACCAA CAAGTCAAGG TGTGGATCAC AAGCAGGTGC AGAGAGAACT AGGCGATGTG
    GTACTACATC TGCACAACCC TACTATCCTT TCTTCCTCCT CAATTGAAGT TCATTGGACA
    GTGGACCAGC AATCCCAATA TATTCAAGGA TATAAAATCC TATACCGGCC AACACCAGCG
    TCCTATGGAG AGTCAGAATG GTTAATTTTT GAAGTGAGGA CTCCAACCAA AAACAGTGTT
    ATCATTCCAG AGCTGAAGAA AGGTGTAAAC TATGAAATCA AGGCCCGCCC TTTTTTTAAT
    GAGTTTCAAG GCGCAGATAG TGAAGTGAAA TTTGCAAAAA CCCTAGAAGA AGCGCCCAGT
    GCTCCACCTC AGAGTGTTTC AGTAACTAAA AATGATGGGA ATGGAACTGC AATTGTAGTC
    ACCTGGCAGC CACCCCCCGA GGATAACCAG AACGGAATGG TTCAAGAATA CAAGGTTTGG
    TGCCTTGGCA ATGAAAGTCG ATACCATATA AACAGGACAG TAGATGGTTC CACCTTTTCA
    GTAGTCATCC CTTCCCTAGT ACCAGGTATT CGATACAGCG TGGAAGTGGC AGCAAGTACT
    GGAGCAGGAC CTGGAGTGAA AAGTGATCCC CAGTTTATCC AGTTAGATTC GCATGGAAAT
    CCTGTGTCTT CAGAAGATCA AGTCAGTTTA GCCCAGCAGA TCTCTGACGT AGTAAAACAG
    CCTGCCTTTA TTGCTGGAAT TGGTGCTGCT TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGTTGTATC GCCACAGAAA GAAGCGAAAT GGACTCACTA GTACTTACGC TGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACCTATC AGAGAGGAGG AGATGCCGTA
    AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC ATCAGTGAAC CAGCCACACA GCCCTGGCTA
    GCAGATACTT GGCCTAACAC AGGGAACAAC CACAATGACT GCTCCATCAA CTGCTGCACC
    TCTGGCAATG GAAACAGTGA TGGCAACCTT ACGACATACA GTCGACCAGC CGATTGTATA
    GCAAATTACA ACAATCAGCT GGACAACAAG CAGACAAACC TGATGTTGCC TGAGTCAACT
    GTATATGGTG ACGTGGACCT AAGCAACAAA ATCAATGAGA TGAAAACCTT CAATAGTCCA
    AATCTAAAGG ATGGAAGATT TGTCAGCCAA CCTGGGCAGC CCACTCCTTA TGCCACCACT
    CAACTCATCC AGTCAAATAT CAGCAATAAT GTTAACAATG GCAGCGGGGA TACGAATGAG
    AAACACTGGA AACCCTCTGT TCAGCAAAAG CAAGAGGTTG CACCCATACA GTACAACATT
    ATGGAGCAAA ACAAATTAAA CAAAGATTAC CGAGTAAATG ACAGCATTGC TCCAACAATT
    CCATACAACC AGTCATATGA CCAGAATACA GGTGGATCCT ACAACAGCTC AGATAGAGGC
    AGTAGCACAT CTGGGAGTCA AGGGCACAAG AAGGGTGCTC GGACTCCAAA GGCTCCCAAG
    CAAGCTGGCA TGAACTGGGC AGATCTTCTT CCACCCCCTC CAGCACATCC ACCTCCCCAT
    AGCAATAGTG AAGATTACAG TCTTTCAGTG GATGAAAGCT ATGACCAAGA AATTCCATGT
    CCAGTCCCAC CTGCAAGGAT GTATCTGCAG CAAGATGAGC TAGAGGAAGA GGAAGAAGAT
    GAGCGAGGTC CTACTCCACC TGTCCGAGGA GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT
    AGTCATCAGT CAACTGCCAC TCTCACCCCT TCTCCCCAGG AAGAACTCCA GCCCATGTTA
    CAGGACTGTC AGGAGGATCT GGGACACATA CAACACCAAC CTGAACGGAG ACGTCAGCCT
    GTGAGCCCTC CACCCCCTCC AAGGCCTATC TCTCCACCAC ATACCTATGG ATATATCTCA
    GGGCCTTTAG TCTCTGACAT GGATACTGAT GCACCAGAAG AGGAAGAAGA TGAGGCAGAT
    ATGGAGGTTG CTAAGATGCA AAACAGAAGA CTCCTTTTGC GTGGCCTTGA GCAGACACCT
    GCTTCAAGTG TTGGTGATTT GGAGAGCTCT GTAACAGGGT CCATGATCAA TGGCTGGGGT
    TCAGCTTCCG AAGAAGATAA CATCTCAAGT GGGCGGTCTA GTGTTAGCTC TTCAGATGGA
    TCATTTTTTA CTGATGCAGA TTTTGCACAG GCTGTTGCAG CAGCTGCAGA ATATGCTGGC
    TTGAAAGTAG CCAGACGTCA AATGCAGGAT GCAGCAGGAG GCCGTAGACA TTTTCATGCA
    TCACATTGTC CCAGACCTAC CAGCCCTGTA TCTACTGACA GCAACATGAG TGCTGTTGTA
    ATACAGAAGG TCCGACCTGC CAAAAAGCAA AAACACCAGC CAGGACATCT GCGCAGAGAA
    GTCTACACAG ATGACTTGCC ACCCCCTCCC GTGCCACCTC CCGCTATAAA ATCACCAACC
    GCCCAGTCCA AATCTCAGCT GGAGGTGCGA CCTGTCATGT TGCCAAAACT GGCTTCTATA
    GAACCGAGGA CAGACAGATC TGCGGAAAGA AAAGGAGTGA GCTACAAGGG GAGGGACGGG
    GTGGATGGGA GACAGCATTC AGACATGCGG ACAAATTCAG GAGAGCGGAG AGAATCACAG
    GAACAGCAAG GCGACGGGAA ACTGCGAGGA AGTAAAGCAC CAAAACGAGA AGGCACACCA
    GCAAAAACTC ATCTTCTTCA AGAGGACATC CTGCCATATT CTAGACCAAC GTTTCCAACG
    TCAAATAATC CCAGAGATCC CAGCTCCTCC AGCTCTATGT CTTCGAGAGG ATCAGGAGGG
    AGACAGAGGG CAGATCAAGC AAATATAGCC CGAAGAAATG TTGCAGAAAT GCAAGTACTG
    GGAGCATATG AACAAGGAGA AGAAGAAGAA GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_416673.5
    CDS802..5658
    Translation

    Target ORF information:

    RefSeq Version XM_416673.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_416673.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGTTGCGG AGTTTGCTCA CTTTTACCTC TGTGGATTAC TCTGCCTCGC CTCAGGCTCC 
    CGTCTTCGGC AAGAAGATTT CCCACCTCGC ATTGTTGAAC ACCCATCTGA TTTAATTGTT
    TCAAAAGGAG AACCAGCAAC TTTAAACTGC AAAGCTGAAG GAAGGCCAAC ACCAACCATT
    GAGTGGTATA AAGGTGGAGA AAGAGTGGAA ACTGACAAGG ATGATCCTCG TTCTCATCGG
    ATGCTGCTGC CCAGCGGATC TTTATTTTTT TTACGCATAG TTCATGGACG CAAGAGTAGG
    CCAGATGAAG GGGTCTATGT CTGCGTGGCA AGGAACTACC TTGGGGAAGC TGTAAGTCAC
    AATGCATCAC TGGAGGTGGC AATTCTACGG GATGACTTCA GACAAAATCC ATCAGATGTA
    ATGGTAGCTG TGGGTGAACC TGCTGTGATG GAATGTCAGC CCCCAAGAGG TCACCCAGAA
    CCCACTATAT CATGGAAAAA AGATGGGACT CCTCTAGATG ACAAAGATGA GAGAATTACA
    ATCAGAGGTG GAAAGCTTAT GATCACATAC ACAAGAAAGA ATGATGCTGG CAAGTACGTT
    TGTGTTGGAA CAAATATGGT TGGAGAACGT GAAAGTGAAG TTGCAGAGCT CACTGTTTTA
    GAAAGACCGT CTTTTGTGAA GAGACCAAGT AATTTGGCTG TAACTGTGGA TGACAGTGCT
    GAGTTTAAAT GTGAGGCAAG AGGTGACCCA GTCCCTACAG TAAGATGGAG GAAAGATGAT
    GGGGAATTAC CAAAAGCGAG ATATGAAATT CGTGATGATC ATACCCTGAA AATCCGGAAA
    GTTATGGCAG GAGACATGGG CTCATATACT TGTGTAGCAG AAAATATGGT TGGAAAAGCT
    GAAGCATCGG CAACTCTAAC AGTGCAAGTT GTGTCTGAGC CTCCACAGTT TGTTGTAAAG
    CCTCGTGATC AGGTAGCTGC ATTGGGAAGA ACAGTGACTT TCCAGTGTGA AGCAACTGGG
    AACCCACAGC CAGCCATCTT TTGGCGGAGA GAGGGAAGTC AGAATCTGCT TTTCTCATAC
    CAGCCCCCCC AGTCATCCAG TCGATTTTCA GTTTCCCAGA CAGGAGACCT CACCATCACC
    AATGTCCAGA GATCTGATGT TGGGTACTAC ATCTGCCAGA CTTTAAATGT TGCAGGAAGC
    ATCATAACAA AAGCCTACTT GGAAGTTACT GATGTGATTG CAGACCGGCC TCCCCCTGTC
    ATTCGACAGG GTCCTGTGAA TCAGACTGTA GCTGTAGATG GTACTTTAGT ACTAAACTGT
    GTGGCCACAG GCACTTTAAC ACCCACTATC CTCTGGAAAA AGGATGGGAC CCTGATCTCT
    ACCCAGGACT CTCGCATCAA ACAGTTGGAG ACAGGAGCAT TACAGATCCG ATATGCCAAG
    CTGGGTGATA CTGGTCGGTA CACCTGCGTT GCTTCAACCC CAAGTGGCGA AGCTACATGG
    AGTGCCTACA TAGAGGTCCA AGAATTTGGA GTCCCAGTGC AGCCACCAAG ACCCACTGAC
    CCAAACTTGA TTCCTAGTGC TCCATCAAAA CCTGAGGTTA CAGATGTCAG CAGAAATACA
    GTAACATTGT CATGGCAACC TAATTTGAAT TCAGGTGCAA CGCCAACCTC TTACATAATT
    GAGGCCTTCA GCCATGCATC TGGTAGCAGC TGGCAAACTG TAGCTGAAAA TGTGAAAACA
    GAGAGTTTTG CAGTTAAAGG GCTAAAACCT AGTGCAATTT ATCTATTCCT TGTGAGGGCA
    GCTAATGCAT ATGGACTAAG TGACCCAAGC CAAATATCTG ATCCAGTGAA AACTCAAGAT
    GTTCCACCAA CAAGTCAAGG TGTGGATCAC AAGCAGGTGC AGAGAGAACT AGGCGATGTG
    GTACTACATC TGCACAACCC TACTATCCTT TCTTCCTCCT CAATTGAAGT TCATTGGACA
    GTGGACCAGC AATCCCAATA TATTCAAGGA TATAAAATCC TATACCGGCC AACACCAGCG
    TCCTATGGAG AGTCAGAATG GTTAATTTTT GAAGTGAGGA CTCCAACCAA AAACAGTGTT
    ATCATTCCAG AGCTGAAGAA AGGTGTAAAC TATGAAATCA AGGCCCGCCC TTTTTTTAAT
    GAGTTTCAAG GCGCAGATAG TGAAGTGAAA TTTGCAAAAA CCCTAGAAGA AGCGCCCAGT
    GCTCCACCTC AGAGTGTTTC AGTAACTAAA AATGATGGGA ATGGAACTGC AATTGTAGTC
    ACCTGGCAGC CACCCCCCGA GGATAACCAG AACGGAATGG TTCAAGAATA CAAGGTTTGG
    TGCCTTGGCA ATGAAAGTCG ATACCATATA AACAGGACAG TAGATGGTTC CACCTTTTCA
    GTAGTCATCC CTTCCCTAGT ACCAGGTATT CGATACAGCG TGGAAGTGGC AGCAAGTACT
    GGAGCAGGAC CTGGAGTGAA AAGTGATCCC CAGTTTATCC AGTTAGATTC GCATGGAAAT
    CCTGTGTCTT CAGAAGATCA AGTCAGTTTA GCCCAGCAGA TCTCTGACGT AGTAAAACAG
    CCTGCCTTTA TTGCTGGAAT TGGTGCTGCT TGTTGGATTA TCCTCATGGT CTTCAGCATC
    TGGTTGTATC GCCACAGAAA GAAGCGAAAT GGACTCACTA GTACTTACGC TGGTATCAGA
    AAAGTCCCGT CTTTTACCTT CACACCAACA GTAACCTATC AGAGAGGAGG AGATGCCGTA
    AGCAGTGGAG GCAGGCCAGG ACTCCTAAAC ATCAGTGAAC CAGCCACACA GCCCTGGCTA
    GCAGATACTT GGCCTAACAC AGGGAACAAC CACAATGACT GCTCCATCAA CTGCTGCACC
    TCTGGCAATG GAAACAGTGA TGGCAACCTT ACGACATACA GTCGACCAGC CGATTGTATA
    GCAAATTACA ACAATCAGCT GGACAACAAG CAGACAAACC TGATGTTGCC TGAGTCAACT
    GTATATGGTG ACGTGGACCT AAGCAACAAA ATCAATGAGA TGAAAACCTT CAATAGTCCA
    AATCTAAAGG ATGGAAGATT TGTCAGCCAA CCTGGGCAGC CCACTCCTTA TGCCACCACT
    CAACTCATCC AGTCAAATAT CAGCAATAAT GTTAACAATG GCAGCGGGGA TACGAATGAG
    AAACACTGGA AACCCTCTGT TCAGCAAAAG CAAGAGGTTG CACCCATACA GTACAACATT
    ATGGAGCAAA ACAAATTAAA CAAAGATTAC CGAGTAAATG ACAGCATTGC TCCAACAATT
    CCATACAACC AGTCATATGA CCAGAATACA GGTGGATCCT ACAACAGCTC AGATAGAGGC
    AGTAGCACAT CTGGGAGTCA AGGGCACAAG AAGGGTGCTC GGACTCCAAA GGCTCCCAAG
    CAAGCTGGCA TGAACTGGGC AGATCTTCTT CCACCCCCTC CAGCACATCC ACCTCCCCAT
    AGCAATAGTG AAGATTACAG TCTTTCAGTG GATGAAAGCT ATGACCAAGA AATTCCATGT
    CCAGTCCCAC CTGCAAGGAT GTATCTGCAG CAAGATGAGC TAGAGGAAGA GGAAGAAGAT
    GAGCGAGGTC CTACTCCACC TGTCCGAGGA GCAGCTTCCT CTCCAGCCGC AGTCTCCTAT
    AGTCATCAGT CAACTGCCAC TCTCACCCCT TCTCCCCAGG AAGAACTCCA GCCCATGTTA
    CAGGACTGTC AGGAGGATCT GGGACACATA CAACACCAAC CTGAACGGAG ACGTCAGCCT
    GTGAGCCCTC CACCCCCTCC AAGGCCTATC TCTCCACCAC ATACCTATGG ATATATCTCA
    GGGCCTTTAG TCTCTGACAT GGATACTGAT GCACCAGAAG AGGAAGAAGA TGAGGCAGAT
    ATGGAGGTTG CTAAGATGCA AAACAGAAGA CTCCTTTTGC GTGGCCTTGA GCAGACACCT
    GCTTCAAGTG TTGGTGATTT GGAGAGCTCT GTAACAGGGT CCATGATCAA TGGCTGGGGT
    TCAGCTTCCG AAGAAGATAA CATCTCAAGT GGGCGGTCTA GTGTTAGCTC TTCAGATGGA
    TCATTTTTTA CTGATGCAGA TTTTGCACAG GCTGTTGCAG CAGCTGCAGA ATATGCTGGC
    TTGAAAGTAG CCAGACGTCA AATGCAGGAT GCAGCAGGAG GCCGTAGACA TTTTCATGCA
    TCACATTGTC CCAGACCTAC CAGCCCTGTA TCTACTGACA GCAACATGAG TGCTGTTGTA
    ATACAGAAGG TCCGACCTGC CAAAAAGCAA AAACACCAGC CAGGACATCT GCGCAGAGAA
    GTCTACACAG ATGACTTGCC ACCCCCTCCC GTGCCACCTC CCGCTATAAA ATCACCAACC
    GCCCAGTCCA AATCTCAGCT GGAGGTGCGA CCTGTCATGT TGCCAAAACT GGCTTCTATA
    GAACCGAGGA CAGACAGATC TGCGGAAAGA AAAGGAGTGA GCTACAAGGG GAGGGACGGG
    GTGGATGGGA GACAGCATTC AGACATGCGG ACAAATTCAG GAGAGCGGAG AGAATCACAG
    GAACAGCAAG GCGACGGGAA ACTGCGAGGA AGTAAAGCAC CAAAACGAGA AGGCACACCA
    GCAAAAACTC ATCTTCTTCA AGAGGACATC CTGCCATATT CTAGACCAAC GTTTCCAACG
    TCAAATAATC CCAGAGATCC CAGCTCCTCC AGCTCTATGT CTTCGAGAGG ATCAGGAGGG
    AGACAGAGGG CAGATCAAGC AAATATAGCC CGAAGAAATG TTGCAGAAAT GCAAGTACTG
    GGAGCATATG AACAAGGAGA AGAAGAAGAA GATGAGATGG AGGAAACAGA AAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30207
    Clone ID Related Accession (Same CDS sequence) XM_015298516.2 , XM_015298516.1
    Accession Version XM_015298516.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298516.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGATTA CCGAGTAAAT
    GACAGCATTG CTCCAACAAT TCCATACAAC CAGTCATATG ACCAGAATAC AGGTGGATCC
    TACAACAGCT CAGATAGAGG CAGTAGCACA TCTGGGAGTC AAGGGCACAA GAAGGGTGCT
    CGGACTCCAA AGGCTCCCAA GCAAGCTGGC ATGAACTGGG CAGATCTTCT TCCACCCCCT
    CCAGCACATC CACCTCCCCA TAGCAATAGT GAAGATTACA GTCTTTCAGT GGATGAAAGC
    TATGACCAAG AAATTCCATG TCCAGTCCCA CCTGCAAGGA TGTATCTGCA GCAAGATGAG
    CTAGAGGAAG AGGAAGAAGA TGAGCGAGGT CCTACTCCAC CTGTCCGAGG AGCAGCTTCC
    TCTCCAGCCG CAGTCTCCTA TAGTCATCAG TCAACTGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAACTCC AGCCCATGTT ACAGGACTGT CAGGAGGATC TGGGACACAT ACAACACCAA
    CCTGAACGGA GACGTCAGCC TGTGAGCCCT CCACCCCCTC CAAGGCCTAT CTCTCCACCA
    CATACCTATG GATATATCTC AGGGCCTTTA GTCTCTGACA TGGATACTGA TGCACCAGAA
    GAGGAAGAAG ATGAGGCAGA TATGGAGGTT GCTAAGATGC AAAACAGAAG ACTCCTTTTG
    CGTGGCCTTG AGCAGACACC TGCTTCAAGT GTTGGTGATT TGGAGAGCTC TGTAACAGGG
    TCCATGATCA ATGGCTGGGG TTCAGCTTCC GAAGAAGATA ACATCTCAAG TGGGCGGTCT
    AGTGTTAGCT CTTCAGATGG ATCATTTTTT ACTGATGCAG ATTTTGCACA GGCTGTTGCA
    GCAGCTGCAG AATATGCTGG CTTGAAAGTA GCCAGACGTC AAATGCAGGA TGCAGCAGGA
    GGCCGTAGAC ATTTTCATGC ATCACATTGT CCCAGACCTA CCAGCCCTGT ATCTACTGAC
    AGCAACATGA GTGCTGTTGT AATACAGAAG GTCCGACCTG CCAAAAAGCA AAAACACCAG
    CCAGGACATC TGCGCAGAGA AGTCTACACA GATGACTTGC CACCCCCTCC CGTGCCACCT
    CCCGCTATAA AATCACCAAC CGCCCAGTCC AAATCTCAGC TGGAGGTGCG ACCTGTCATG
    TTGCCAAAAC TGGCTTCTAT AGAACCGAGG ACAGACAGAT CTGCGGAAAG AAAAGGAGTG
    AGCTACAAGG GGAGGGACGG GGTGGATGGG AGACAGCATT CAGACATGCG GACAAATTCA
    GGAGAGCGGA GAGAATCACA GGAACAGCAA GGCGACGGGA AACTGCGAGG AAGTAAAGCA
    CCAAAACGAG AAGGCACACC AGCAAAAACT CATCTTCTTC AAGAGGACAT CCTGCCATAT
    TCTAGACCAA CGTTTCCAAC GTCAAATAAT CCCAGAGATC CCAGCTCCTC CAGCTCTATG
    TCTTCGAGAG GATCAGGAGG GAGACAGAGG GCAGATCAAG CAAATATAGC CCGAAGAAAT
    GTTGCAGAAA TGCAAGTACT GGGAGCATAT GAACAAGGAG AAGAAGAAGA AGATGAGATG
    GAGGAAACAG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154002.1
    CDS324..4961
    Translation

    Target ORF information:

    RefSeq Version XM_015298516.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298516.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGATTA CCGAGTAAAT
    GACAGCATTG CTCCAACAAT TCCATACAAC CAGTCATATG ACCAGAATAC AGGTGGATCC
    TACAACAGCT CAGATAGAGG CAGTAGCACA TCTGGGAGTC AAGGGCACAA GAAGGGTGCT
    CGGACTCCAA AGGCTCCCAA GCAAGCTGGC ATGAACTGGG CAGATCTTCT TCCACCCCCT
    CCAGCACATC CACCTCCCCA TAGCAATAGT GAAGATTACA GTCTTTCAGT GGATGAAAGC
    TATGACCAAG AAATTCCATG TCCAGTCCCA CCTGCAAGGA TGTATCTGCA GCAAGATGAG
    CTAGAGGAAG AGGAAGAAGA TGAGCGAGGT CCTACTCCAC CTGTCCGAGG AGCAGCTTCC
    TCTCCAGCCG CAGTCTCCTA TAGTCATCAG TCAACTGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAACTCC AGCCCATGTT ACAGGACTGT CAGGAGGATC TGGGACACAT ACAACACCAA
    CCTGAACGGA GACGTCAGCC TGTGAGCCCT CCACCCCCTC CAAGGCCTAT CTCTCCACCA
    CATACCTATG GATATATCTC AGGGCCTTTA GTCTCTGACA TGGATACTGA TGCACCAGAA
    GAGGAAGAAG ATGAGGCAGA TATGGAGGTT GCTAAGATGC AAAACAGAAG ACTCCTTTTG
    CGTGGCCTTG AGCAGACACC TGCTTCAAGT GTTGGTGATT TGGAGAGCTC TGTAACAGGG
    TCCATGATCA ATGGCTGGGG TTCAGCTTCC GAAGAAGATA ACATCTCAAG TGGGCGGTCT
    AGTGTTAGCT CTTCAGATGG ATCATTTTTT ACTGATGCAG ATTTTGCACA GGCTGTTGCA
    GCAGCTGCAG AATATGCTGG CTTGAAAGTA GCCAGACGTC AAATGCAGGA TGCAGCAGGA
    GGCCGTAGAC ATTTTCATGC ATCACATTGT CCCAGACCTA CCAGCCCTGT ATCTACTGAC
    AGCAACATGA GTGCTGTTGT AATACAGAAG GTCCGACCTG CCAAAAAGCA AAAACACCAG
    CCAGGACATC TGCGCAGAGA AGTCTACACA GATGACTTGC CACCCCCTCC CGTGCCACCT
    CCCGCTATAA AATCACCAAC CGCCCAGTCC AAATCTCAGC TGGAGGTGCG ACCTGTCATG
    TTGCCAAAAC TGGCTTCTAT AGAACCGAGG ACAGACAGAT CTGCGGAAAG AAAAGGAGTG
    AGCTACAAGG GGAGGGACGG GGTGGATGGG AGACAGCATT CAGACATGCG GACAAATTCA
    GGAGAGCGGA GAGAATCACA GGAACAGCAA GGCGACGGGA AACTGCGAGG AAGTAAAGCA
    CCAAAACGAG AAGGCACACC AGCAAAAACT CATCTTCTTC AAGAGGACAT CCTGCCATAT
    TCTAGACCAA CGTTTCCAAC GTCAAATAAT CCCAGAGATC CCAGCTCCTC CAGCTCTATG
    TCTTCGAGAG GATCAGGAGG GAGACAGAGG GCAGATCAAG CAAATATAGC CCGAAGAAAT
    GTTGCAGAAA TGCAAGTACT GGGAGCATAT GAACAAGGAG AAGAAGAAGA AGATGAGATG
    GAGGAAACAG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30207
    Clone ID Related Accession (Same CDS sequence) XM_015298516.2 , XM_015298516.1
    Accession Version XM_015298516.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGATTA CCGAGTAAAT
    GACAGCATTG CTCCAACAAT TCCATACAAC CAGTCATATG ACCAGAATAC AGGTGGATCC
    TACAACAGCT CAGATAGAGG CAGTAGCACA TCTGGGAGTC AAGGGCACAA GAAGGGTGCT
    CGGACTCCAA AGGCTCCCAA GCAAGCTGGC ATGAACTGGG CAGATCTTCT TCCACCCCCT
    CCAGCACATC CACCTCCCCA TAGCAATAGT GAAGATTACA GTCTTTCAGT GGATGAAAGC
    TATGACCAAG AAATTCCATG TCCAGTCCCA CCTGCAAGGA TGTATCTGCA GCAAGATGAG
    CTAGAGGAAG AGGAAGAAGA TGAGCGAGGT CCTACTCCAC CTGTCCGAGG AGCAGCTTCC
    TCTCCAGCCG CAGTCTCCTA TAGTCATCAG TCAACTGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAACTCC AGCCCATGTT ACAGGACTGT CAGGAGGATC TGGGACACAT ACAACACCAA
    CCTGAACGGA GACGTCAGCC TGTGAGCCCT CCACCCCCTC CAAGGCCTAT CTCTCCACCA
    CATACCTATG GATATATCTC AGGGCCTTTA GTCTCTGACA TGGATACTGA TGCACCAGAA
    GAGGAAGAAG ATGAGGCAGA TATGGAGGTT GCTAAGATGC AAAACAGAAG ACTCCTTTTG
    CGTGGCCTTG AGCAGACACC TGCTTCAAGT GTTGGTGATT TGGAGAGCTC TGTAACAGGG
    TCCATGATCA ATGGCTGGGG TTCAGCTTCC GAAGAAGATA ACATCTCAAG TGGGCGGTCT
    AGTGTTAGCT CTTCAGATGG ATCATTTTTT ACTGATGCAG ATTTTGCACA GGCTGTTGCA
    GCAGCTGCAG AATATGCTGG CTTGAAAGTA GCCAGACGTC AAATGCAGGA TGCAGCAGGA
    GGCCGTAGAC ATTTTCATGC ATCACATTGT CCCAGACCTA CCAGCCCTGT ATCTACTGAC
    AGCAACATGA GTGCTGTTGT AATACAGAAG GTCCGACCTG CCAAAAAGCA AAAACACCAG
    CCAGGACATC TGCGCAGAGA AGTCTACACA GATGACTTGC CACCCCCTCC CGTGCCACCT
    CCCGCTATAA AATCACCAAC CGCCCAGTCC AAATCTCAGC TGGAGGTGCG ACCTGTCATG
    TTGCCAAAAC TGGCTTCTAT AGAACCGAGG ACAGACAGAT CTGCGGAAAG AAAAGGAGTG
    AGCTACAAGG GGAGGGACGG GGTGGATGGG AGACAGCATT CAGACATGCG GACAAATTCA
    GGAGAGCGGA GAGAATCACA GGAACAGCAA GGCGACGGGA AACTGCGAGG AAGTAAAGCA
    CCAAAACGAG AAGGCACACC AGCAAAAACT CATCTTCTTC AAGAGGACAT CCTGCCATAT
    TCTAGACCAA CGTTTCCAAC GTCAAATAAT CCCAGAGATC CCAGCTCCTC CAGCTCTATG
    TCTTCGAGAG GATCAGGAGG GAGACAGAGG GCAGATCAAG CAAATATAGC CCGAAGAAAT
    GTTGCAGAAA TGCAAGTACT GGGAGCATAT GAACAAGGAG AAGAAGAAGA AGATGAGATG
    GAGGAAACAG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015154002.1
    CDS322..4959
    Translation

    Target ORF information:

    RefSeq Version XM_015298516.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298516.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGATTA CCGAGTAAAT
    GACAGCATTG CTCCAACAAT TCCATACAAC CAGTCATATG ACCAGAATAC AGGTGGATCC
    TACAACAGCT CAGATAGAGG CAGTAGCACA TCTGGGAGTC AAGGGCACAA GAAGGGTGCT
    CGGACTCCAA AGGCTCCCAA GCAAGCTGGC ATGAACTGGG CAGATCTTCT TCCACCCCCT
    CCAGCACATC CACCTCCCCA TAGCAATAGT GAAGATTACA GTCTTTCAGT GGATGAAAGC
    TATGACCAAG AAATTCCATG TCCAGTCCCA CCTGCAAGGA TGTATCTGCA GCAAGATGAG
    CTAGAGGAAG AGGAAGAAGA TGAGCGAGGT CCTACTCCAC CTGTCCGAGG AGCAGCTTCC
    TCTCCAGCCG CAGTCTCCTA TAGTCATCAG TCAACTGCCA CTCTCACCCC TTCTCCCCAG
    GAAGAACTCC AGCCCATGTT ACAGGACTGT CAGGAGGATC TGGGACACAT ACAACACCAA
    CCTGAACGGA GACGTCAGCC TGTGAGCCCT CCACCCCCTC CAAGGCCTAT CTCTCCACCA
    CATACCTATG GATATATCTC AGGGCCTTTA GTCTCTGACA TGGATACTGA TGCACCAGAA
    GAGGAAGAAG ATGAGGCAGA TATGGAGGTT GCTAAGATGC AAAACAGAAG ACTCCTTTTG
    CGTGGCCTTG AGCAGACACC TGCTTCAAGT GTTGGTGATT TGGAGAGCTC TGTAACAGGG
    TCCATGATCA ATGGCTGGGG TTCAGCTTCC GAAGAAGATA ACATCTCAAG TGGGCGGTCT
    AGTGTTAGCT CTTCAGATGG ATCATTTTTT ACTGATGCAG ATTTTGCACA GGCTGTTGCA
    GCAGCTGCAG AATATGCTGG CTTGAAAGTA GCCAGACGTC AAATGCAGGA TGCAGCAGGA
    GGCCGTAGAC ATTTTCATGC ATCACATTGT CCCAGACCTA CCAGCCCTGT ATCTACTGAC
    AGCAACATGA GTGCTGTTGT AATACAGAAG GTCCGACCTG CCAAAAAGCA AAAACACCAG
    CCAGGACATC TGCGCAGAGA AGTCTACACA GATGACTTGC CACCCCCTCC CGTGCCACCT
    CCCGCTATAA AATCACCAAC CGCCCAGTCC AAATCTCAGC TGGAGGTGCG ACCTGTCATG
    TTGCCAAAAC TGGCTTCTAT AGAACCGAGG ACAGACAGAT CTGCGGAAAG AAAAGGAGTG
    AGCTACAAGG GGAGGGACGG GGTGGATGGG AGACAGCATT CAGACATGCG GACAAATTCA
    GGAGAGCGGA GAGAATCACA GGAACAGCAA GGCGACGGGA AACTGCGAGG AAGTAAAGCA
    CCAAAACGAG AAGGCACACC AGCAAAAACT CATCTTCTTC AAGAGGACAT CCTGCCATAT
    TCTAGACCAA CGTTTCCAAC GTCAAATAAT CCCAGAGATC CCAGCTCCTC CAGCTCTATG
    TCTTCGAGAG GATCAGGAGG GAGACAGAGG GCAGATCAAG CAAATATAGC CCGAAGAAAT
    GTTGCAGAAA TGCAAGTACT GGGAGCATAT GAACAAGGAG AAGAAGAAGA AGATGAGATG
    GAGGAAACAG AAAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa02612
    Clone ID Related Accession (Same CDS sequence) XM_004938345.2 , XM_004938345.3
    Accession Version XM_004938345.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004938345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGCTATG ACCAAGAAAT TCCATGTCCA
    GTCCCACCTG CAAGGATGTA TCTGCAGCAA GATGAGCTAG AGGAAGAGGA AGAAGATGAG
    CGAGGTCCTA CTCCACCTGT CCGAGGAGCA GCTTCCTCTC CAGCCGCAGT CTCCTATAGT
    CATCAGTCAA CTGCCACTCT CACCCCTTCT CCCCAGGAAG AACTCCAGCC CATGTTACAG
    GACTGTCAGG AGGATCTGGG ACACATACAA CACCAACCTG AACGGAGACG TCAGCCTGTG
    AGCCCTCCAC CCCCTCCAAG GCCTATCTCT CCACCACATA CCTATGGATA TATCTCAGGG
    CCTTTAGTCT CTGACATGGA TACTGATGCA CCAGAAGAGG AAGAAGATGA GGCAGATATG
    GAGGTTGCTA AGATGCAAAA CAGAAGACTC CTTTTGCGTG GCCTTGAGCA GACACCTGCT
    TCAAGTGTTG GTGATTTGGA GAGCTCTGTA ACAGGGTCCA TGATCAATGG CTGGGGTTCA
    GCTTCCGAAG AAGATAACAT CTCAAGTGGG CGGTCTAGTG TTAGCTCTTC AGATGGATCA
    TTTTTTACTG ATGCAGATTT TGCACAGGCT GTTGCAGCAG CTGCAGAATA TGCTGGCTTG
    AAAGTAGCCA GACGTCAAAT GCAGGATGCA GCAGGAGGCC GTAGACATTT TCATGCATCA
    CATTGTCCCA GACCTACCAG CCCTGTATCT ACTGACAGCA ACATGAGTGC TGTTGTAATA
    CAGAAGGTCC GACCTGCCAA AAAGCAAAAA CACCAGCCAG GACATCTGCG CAGAGAAGTC
    TACACAGATG ACTTGCCACC CCCTCCCGTG CCACCTCCCG CTATAAAATC ACCAACCGCC
    CAGTCCAAAT CTCAGCTGGA GGTGCGACCT GTCATGTTGC CAAAACTGGC TTCTATAGAA
    CCGAGGACAG ACAGATCTGC GGAAAGAAAA GGAGTGAGCT ACAAGGGGAG GGACGGGGTG
    GATGGGAGAC AGCATTCAGA CATGCGGACA AATTCAGGAG AGCGGAGAGA ATCACAGGAA
    CAGCAAGGCG ACGGGAAACT GCGAGGAAGT AAAGCACCAA AACGAGAAGG CACACCAGCA
    AAAACTCATC TTCTTCAAGA GGACATCCTG CCATATTCTA GACCAACGTT TCCAACGTCA
    AATAATCCCA GAGATCCCAG CTCCTCCAGC TCTATGTCTT CGAGAGGATC AGGAGGGAGA
    CAGAGGGCAG ATCAAGCAAA TATAGCCCGA AGAAATGTTG CAGAAATGCA AGTACTGGGA
    GCATATGAAC AAGGAGAAGA AGAAGAAGAT GAGATGGAGG AAACAGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004938402.1
    CDS322..5295
    Translation

    Target ORF information:

    RefSeq Version XM_004938345.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004938345.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGCTATG ACCAAGAAAT TCCATGTCCA
    GTCCCACCTG CAAGGATGTA TCTGCAGCAA GATGAGCTAG AGGAAGAGGA AGAAGATGAG
    CGAGGTCCTA CTCCACCTGT CCGAGGAGCA GCTTCCTCTC CAGCCGCAGT CTCCTATAGT
    CATCAGTCAA CTGCCACTCT CACCCCTTCT CCCCAGGAAG AACTCCAGCC CATGTTACAG
    GACTGTCAGG AGGATCTGGG ACACATACAA CACCAACCTG AACGGAGACG TCAGCCTGTG
    AGCCCTCCAC CCCCTCCAAG GCCTATCTCT CCACCACATA CCTATGGATA TATCTCAGGG
    CCTTTAGTCT CTGACATGGA TACTGATGCA CCAGAAGAGG AAGAAGATGA GGCAGATATG
    GAGGTTGCTA AGATGCAAAA CAGAAGACTC CTTTTGCGTG GCCTTGAGCA GACACCTGCT
    TCAAGTGTTG GTGATTTGGA GAGCTCTGTA ACAGGGTCCA TGATCAATGG CTGGGGTTCA
    GCTTCCGAAG AAGATAACAT CTCAAGTGGG CGGTCTAGTG TTAGCTCTTC AGATGGATCA
    TTTTTTACTG ATGCAGATTT TGCACAGGCT GTTGCAGCAG CTGCAGAATA TGCTGGCTTG
    AAAGTAGCCA GACGTCAAAT GCAGGATGCA GCAGGAGGCC GTAGACATTT TCATGCATCA
    CATTGTCCCA GACCTACCAG CCCTGTATCT ACTGACAGCA ACATGAGTGC TGTTGTAATA
    CAGAAGGTCC GACCTGCCAA AAAGCAAAAA CACCAGCCAG GACATCTGCG CAGAGAAGTC
    TACACAGATG ACTTGCCACC CCCTCCCGTG CCACCTCCCG CTATAAAATC ACCAACCGCC
    CAGTCCAAAT CTCAGCTGGA GGTGCGACCT GTCATGTTGC CAAAACTGGC TTCTATAGAA
    CCGAGGACAG ACAGATCTGC GGAAAGAAAA GGAGTGAGCT ACAAGGGGAG GGACGGGGTG
    GATGGGAGAC AGCATTCAGA CATGCGGACA AATTCAGGAG AGCGGAGAGA ATCACAGGAA
    CAGCAAGGCG ACGGGAAACT GCGAGGAAGT AAAGCACCAA AACGAGAAGG CACACCAGCA
    AAAACTCATC TTCTTCAAGA GGACATCCTG CCATATTCTA GACCAACGTT TCCAACGTCA
    AATAATCCCA GAGATCCCAG CTCCTCCAGC TCTATGTCTT CGAGAGGATC AGGAGGGAGA
    CAGAGGGCAG ATCAAGCAAA TATAGCCCGA AGAAATGTTG CAGAAATGCA AGTACTGGGA
    GCATATGAAC AAGGAGAAGA AGAAGAAGAT GAGATGGAGG AAACAGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa02612
    Clone ID Related Accession (Same CDS sequence) XM_004938345.2 , XM_004938345.3
    Accession Version XM_004938345.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004938345.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGCTATG ACCAAGAAAT TCCATGTCCA
    GTCCCACCTG CAAGGATGTA TCTGCAGCAA GATGAGCTAG AGGAAGAGGA AGAAGATGAG
    CGAGGTCCTA CTCCACCTGT CCGAGGAGCA GCTTCCTCTC CAGCCGCAGT CTCCTATAGT
    CATCAGTCAA CTGCCACTCT CACCCCTTCT CCCCAGGAAG AACTCCAGCC CATGTTACAG
    GACTGTCAGG AGGATCTGGG ACACATACAA CACCAACCTG AACGGAGACG TCAGCCTGTG
    AGCCCTCCAC CCCCTCCAAG GCCTATCTCT CCACCACATA CCTATGGATA TATCTCAGGG
    CCTTTAGTCT CTGACATGGA TACTGATGCA CCAGAAGAGG AAGAAGATGA GGCAGATATG
    GAGGTTGCTA AGATGCAAAA CAGAAGACTC CTTTTGCGTG GCCTTGAGCA GACACCTGCT
    TCAAGTGTTG GTGATTTGGA GAGCTCTGTA ACAGGGTCCA TGATCAATGG CTGGGGTTCA
    GCTTCCGAAG AAGATAACAT CTCAAGTGGG CGGTCTAGTG TTAGCTCTTC AGATGGATCA
    TTTTTTACTG ATGCAGATTT TGCACAGGCT GTTGCAGCAG CTGCAGAATA TGCTGGCTTG
    AAAGTAGCCA GACGTCAAAT GCAGGATGCA GCAGGAGGCC GTAGACATTT TCATGCATCA
    CATTGTCCCA GACCTACCAG CCCTGTATCT ACTGACAGCA ACATGAGTGC TGTTGTAATA
    CAGAAGGTCC GACCTGCCAA AAAGCAAAAA CACCAGCCAG GACATCTGCG CAGAGAAGTC
    TACACAGATG ACTTGCCACC CCCTCCCGTG CCACCTCCCG CTATAAAATC ACCAACCGCC
    CAGTCCAAAT CTCAGCTGGA GGTGCGACCT GTCATGTTGC CAAAACTGGC TTCTATAGAA
    CCGAGGACAG ACAGATCTGC GGAAAGAAAA GGAGTGAGCT ACAAGGGGAG GGACGGGGTG
    GATGGGAGAC AGCATTCAGA CATGCGGACA AATTCAGGAG AGCGGAGAGA ATCACAGGAA
    CAGCAAGGCG ACGGGAAACT GCGAGGAAGT AAAGCACCAA AACGAGAAGG CACACCAGCA
    AAAACTCATC TTCTTCAAGA GGACATCCTG CCATATTCTA GACCAACGTT TCCAACGTCA
    AATAATCCCA GAGATCCCAG CTCCTCCAGC TCTATGTCTT CGAGAGGATC AGGAGGGAGA
    CAGAGGGCAG ATCAAGCAAA TATAGCCCGA AGAAATGTTG CAGAAATGCA AGTACTGGGA
    GCATATGAAC AAGGAGAAGA AGAAGAAGAT GAGATGGAGG AAACAGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004938402.1
    CDS325..5298
    Translation

    Target ORF information:

    RefSeq Version XM_004938345.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004938345.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGCTATG ACCAAGAAAT TCCATGTCCA
    GTCCCACCTG CAAGGATGTA TCTGCAGCAA GATGAGCTAG AGGAAGAGGA AGAAGATGAG
    CGAGGTCCTA CTCCACCTGT CCGAGGAGCA GCTTCCTCTC CAGCCGCAGT CTCCTATAGT
    CATCAGTCAA CTGCCACTCT CACCCCTTCT CCCCAGGAAG AACTCCAGCC CATGTTACAG
    GACTGTCAGG AGGATCTGGG ACACATACAA CACCAACCTG AACGGAGACG TCAGCCTGTG
    AGCCCTCCAC CCCCTCCAAG GCCTATCTCT CCACCACATA CCTATGGATA TATCTCAGGG
    CCTTTAGTCT CTGACATGGA TACTGATGCA CCAGAAGAGG AAGAAGATGA GGCAGATATG
    GAGGTTGCTA AGATGCAAAA CAGAAGACTC CTTTTGCGTG GCCTTGAGCA GACACCTGCT
    TCAAGTGTTG GTGATTTGGA GAGCTCTGTA ACAGGGTCCA TGATCAATGG CTGGGGTTCA
    GCTTCCGAAG AAGATAACAT CTCAAGTGGG CGGTCTAGTG TTAGCTCTTC AGATGGATCA
    TTTTTTACTG ATGCAGATTT TGCACAGGCT GTTGCAGCAG CTGCAGAATA TGCTGGCTTG
    AAAGTAGCCA GACGTCAAAT GCAGGATGCA GCAGGAGGCC GTAGACATTT TCATGCATCA
    CATTGTCCCA GACCTACCAG CCCTGTATCT ACTGACAGCA ACATGAGTGC TGTTGTAATA
    CAGAAGGTCC GACCTGCCAA AAAGCAAAAA CACCAGCCAG GACATCTGCG CAGAGAAGTC
    TACACAGATG ACTTGCCACC CCCTCCCGTG CCACCTCCCG CTATAAAATC ACCAACCGCC
    CAGTCCAAAT CTCAGCTGGA GGTGCGACCT GTCATGTTGC CAAAACTGGC TTCTATAGAA
    CCGAGGACAG ACAGATCTGC GGAAAGAAAA GGAGTGAGCT ACAAGGGGAG GGACGGGGTG
    GATGGGAGAC AGCATTCAGA CATGCGGACA AATTCAGGAG AGCGGAGAGA ATCACAGGAA
    CAGCAAGGCG ACGGGAAACT GCGAGGAAGT AAAGCACCAA AACGAGAAGG CACACCAGCA
    AAAACTCATC TTCTTCAAGA GGACATCCTG CCATATTCTA GACCAACGTT TCCAACGTCA
    AATAATCCCA GAGATCCCAG CTCCTCCAGC TCTATGTCTT CGAGAGGATC AGGAGGGAGA
    CAGAGGGCAG ATCAAGCAAA TATAGCCCGA AGAAATGTTG CAGAAATGCA AGTACTGGGA
    GCATATGAAC AAGGAGAAGA AGAAGAAGAT GAGATGGAGG AAACAGAAAG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30206
    Clone ID Related Accession (Same CDS sequence) XM_015298510.2 , XM_015298510.1
    Accession Version XM_015298510.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015298510.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGACGTC AGCCTGTGAG CCCTCCACCC
    CCTCCAAGGC CTATCTCTCC ACCACATACC TATGGATATA TCTCAGGGCC TTTAGTCTCT
    GACATGGATA CTGATGCACC AGAAGAGGAA GAAGATGAGG CAGATATGGA GGTTGCTAAG
    ATGCAAAACA GAAGACTCCT TTTGCGTGGC CTTGAGCAGA CACCTGCTTC AAGTGTTGGT
    GATTTGGAGA GCTCTGTAAC AGGGTCCATG ATCAATGGCT GGGGTTCAGC TTCCGAAGAA
    GATAACATCT CAAGTGGGCG GTCTAGTGTT AGCTCTTCAG ATGGATCATT TTTTACTGAT
    GCAGATTTTG CACAGGCTGT TGCAGCAGCT GCAGAATATG CTGGCTTGAA AGTAGCCAGA
    CGTCAAATGC AGGATGCAGC AGGAGGCCGT AGACATTTTC ATGCATCACA TTGTCCCAGA
    CCTACCAGCC CTGTATCTAC TGACAGCAAC ATGAGTGCTG TTGTAATACA GAAGGTCCGA
    CCTGCCAAAA AGCAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGTCTA CACAGATGAC
    TTGCCACCCC CTCCCGTGCC ACCTCCCGCT ATAAAATCAC CAACCGCCCA GTCCAAATCT
    CAGCTGGAGG TGCGACCTGT CATGTTGCCA AAACTGGCTT CTATAGAACC GAGGACAGAC
    AGATCTGCGG AAAGAAAAGG AGTGAGCTAC AAGGGGAGGG ACGGGGTGGA TGGGAGACAG
    CATTCAGACA TGCGGACAAA TTCAGGAGAG CGGAGAGAAT CACAGGAACA GCAAGGCGAC
    GGGAAACTGC GAGGAAGTAA AGCACCAAAA CGAGAAGGCA CACCAGCAAA AACTCATCTT
    CTTCAAGAGG ACATCCTGCC ATATTCTAGA CCAACGTTTC CAACGTCAAA TAATCCCAGA
    GATCCCAGCT CCTCCAGCTC TATGTCTTCG AGAGGATCAG GAGGGAGACA GAGGGCAGAT
    CAAGCAAATA TAGCCCGAAG AAATGTTGCA GAAATGCAAG TACTGGGAGC ATATGAACAA
    GGAGAAGAAG AAGAAGATGA GATGGAGGAA ACAGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153996.1
    CDS328..5049
    Translation

    Target ORF information:

    RefSeq Version XM_015298510.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout guidance receptor 1 (ROBO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298510.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGACGTC AGCCTGTGAG CCCTCCACCC
    CCTCCAAGGC CTATCTCTCC ACCACATACC TATGGATATA TCTCAGGGCC TTTAGTCTCT
    GACATGGATA CTGATGCACC AGAAGAGGAA GAAGATGAGG CAGATATGGA GGTTGCTAAG
    ATGCAAAACA GAAGACTCCT TTTGCGTGGC CTTGAGCAGA CACCTGCTTC AAGTGTTGGT
    GATTTGGAGA GCTCTGTAAC AGGGTCCATG ATCAATGGCT GGGGTTCAGC TTCCGAAGAA
    GATAACATCT CAAGTGGGCG GTCTAGTGTT AGCTCTTCAG ATGGATCATT TTTTACTGAT
    GCAGATTTTG CACAGGCTGT TGCAGCAGCT GCAGAATATG CTGGCTTGAA AGTAGCCAGA
    CGTCAAATGC AGGATGCAGC AGGAGGCCGT AGACATTTTC ATGCATCACA TTGTCCCAGA
    CCTACCAGCC CTGTATCTAC TGACAGCAAC ATGAGTGCTG TTGTAATACA GAAGGTCCGA
    CCTGCCAAAA AGCAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGTCTA CACAGATGAC
    TTGCCACCCC CTCCCGTGCC ACCTCCCGCT ATAAAATCAC CAACCGCCCA GTCCAAATCT
    CAGCTGGAGG TGCGACCTGT CATGTTGCCA AAACTGGCTT CTATAGAACC GAGGACAGAC
    AGATCTGCGG AAAGAAAAGG AGTGAGCTAC AAGGGGAGGG ACGGGGTGGA TGGGAGACAG
    CATTCAGACA TGCGGACAAA TTCAGGAGAG CGGAGAGAAT CACAGGAACA GCAAGGCGAC
    GGGAAACTGC GAGGAAGTAA AGCACCAAAA CGAGAAGGCA CACCAGCAAA AACTCATCTT
    CTTCAAGAGG ACATCCTGCC ATATTCTAGA CCAACGTTTC CAACGTCAAA TAATCCCAGA
    GATCCCAGCT CCTCCAGCTC TATGTCTTCG AGAGGATCAG GAGGGAGACA GAGGGCAGAT
    CAAGCAAATA TAGCCCGAAG AAATGTTGCA GAAATGCAAG TACTGGGAGC ATATGAACAA
    GGAGAAGAAG AAGAAGATGA GATGGAGGAA ACAGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa30206
    Clone ID Related Accession (Same CDS sequence) XM_015298510.2 , XM_015298510.1
    Accession Version XM_015298510.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4722bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product roundabout homolog 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455730.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGACGTC AGCCTGTGAG CCCTCCACCC
    CCTCCAAGGC CTATCTCTCC ACCACATACC TATGGATATA TCTCAGGGCC TTTAGTCTCT
    GACATGGATA CTGATGCACC AGAAGAGGAA GAAGATGAGG CAGATATGGA GGTTGCTAAG
    ATGCAAAACA GAAGACTCCT TTTGCGTGGC CTTGAGCAGA CACCTGCTTC AAGTGTTGGT
    GATTTGGAGA GCTCTGTAAC AGGGTCCATG ATCAATGGCT GGGGTTCAGC TTCCGAAGAA
    GATAACATCT CAAGTGGGCG GTCTAGTGTT AGCTCTTCAG ATGGATCATT TTTTACTGAT
    GCAGATTTTG CACAGGCTGT TGCAGCAGCT GCAGAATATG CTGGCTTGAA AGTAGCCAGA
    CGTCAAATGC AGGATGCAGC AGGAGGCCGT AGACATTTTC ATGCATCACA TTGTCCCAGA
    CCTACCAGCC CTGTATCTAC TGACAGCAAC ATGAGTGCTG TTGTAATACA GAAGGTCCGA
    CCTGCCAAAA AGCAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGTCTA CACAGATGAC
    TTGCCACCCC CTCCCGTGCC ACCTCCCGCT ATAAAATCAC CAACCGCCCA GTCCAAATCT
    CAGCTGGAGG TGCGACCTGT CATGTTGCCA AAACTGGCTT CTATAGAACC GAGGACAGAC
    AGATCTGCGG AAAGAAAAGG AGTGAGCTAC AAGGGGAGGG ACGGGGTGGA TGGGAGACAG
    CATTCAGACA TGCGGACAAA TTCAGGAGAG CGGAGAGAAT CACAGGAACA GCAAGGCGAC
    GGGAAACTGC GAGGAAGTAA AGCACCAAAA CGAGAAGGCA CACCAGCAAA AACTCATCTT
    CTTCAAGAGG ACATCCTGCC ATATTCTAGA CCAACGTTTC CAACGTCAAA TAATCCCAGA
    GATCCCAGCT CCTCCAGCTC TATGTCTTCG AGAGGATCAG GAGGGAGACA GAGGGCAGAT
    CAAGCAAATA TAGCCCGAAG AAATGTTGCA GAAATGCAAG TACTGGGAGC ATATGAACAA
    GGAGAAGAAG AAGAAGATGA GATGGAGGAA ACAGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015153996.1
    CDS326..5047
    Translation

    Target ORF information:

    RefSeq Version XM_015298510.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus roundabout, axon guidance receptor, homolog 1 (Drosophila) (ROBO1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015298510.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAAATGGA AACATTATCC TTTTTTGGTC ATAGTAGCTG CATGCAGCCT TTCCAGATGT 
    CATTACTTAC TGGGTCAGCT TATTCCAGAC TCTGACGACC TAGAGAGGGG GAACGACAGC
    GGGACGCCAC TCCCCACCTC TGATAATGGT GACAATTCGC TGGGCTACAC AGGCTCCCGT
    CTTCGGCAAG AAGATTTCCC ACCTCGCATT GTTGAACACC CATCTGATTT AATTGTTTCA
    AAAGGAGAAC CAGCAACTTT AAACTGCAAA GCTGAAGGAA GGCCAACACC AACCATTGAG
    TGGTATAAAG GTGGAGAAAG AGTGGAAACT GACAAGGATG ATCCTCGTTC TCATCGGATG
    CTGCTGCCCA GCGGATCTTT ATTTTTTTTA CGCATAGTTC ATGGACGCAA GAGTAGGCCA
    GATGAAGGGG TCTATGTCTG CGTGGCAAGG AACTACCTTG GGGAAGCTGT AAGTCACAAT
    GCATCACTGG AGGTGGCAAT TCTACGGGAT GACTTCAGAC AAAATCCATC AGATGTAATG
    GTAGCTGTGG GTGAACCTGC TGTGATGGAA TGTCAGCCCC CAAGAGGTCA CCCAGAACCC
    ACTATATCAT GGAAAAAAGA TGGGACTCCT CTAGATGACA AAGATGAGAG AATTACAATC
    AGAGGTGGAA AGCTTATGAT CACATACACA AGAAAGAATG ATGCTGGCAA GTACGTTTGT
    GTTGGAACAA ATATGGTTGG AGAACGTGAA AGTGAAGTTG CAGAGCTCAC TGTTTTAGAA
    AGACCGTCTT TTGTGAAGAG ACCAAGTAAT TTGGCTGTAA CTGTGGATGA CAGTGCTGAG
    TTTAAATGTG AGGCAAGAGG TGACCCAGTC CCTACAGTAA GATGGAGGAA AGATGATGGG
    GAATTACCAA AAGCGAGATA TGAAATTCGT GATGATCATA CCCTGAAAAT CCGGAAAGTT
    ATGGCAGGAG ACATGGGCTC ATATACTTGT GTAGCAGAAA ATATGGTTGG AAAAGCTGAA
    GCATCGGCAA CTCTAACAGT GCAAGTTGTG TCTGAGCCTC CACAGTTTGT TGTAAAGCCT
    CGTGATCAGG TAGCTGCATT GGGAAGAACA GTGACTTTCC AGTGTGAAGC AACTGGGAAC
    CCACAGCCAG CCATCTTTTG GCGGAGAGAG GGAAGTCAGA ATCTGCTTTT CTCATACCAG
    CCCCCCCAGT CATCCAGTCG ATTTTCAGTT TCCCAGACAG GAGACCTCAC CATCACCAAT
    GTCCAGAGAT CTGATGTTGG GTACTACATC TGCCAGACTT TAAATGTTGC AGGAAGCATC
    ATAACAAAAG CCTACTTGGA AGTTACTGAT GTGATTGCAG ACCGGCCTCC CCCTGTCATT
    CGACAGGGTC CTGTGAATCA GACTGTAGCT GTAGATGGTA CTTTAGTACT AAACTGTGTG
    GCCACAGGCA CTTTAACACC CACTATCCTC TGGAAAAAGG ATGGGACCCT GATCTCTACC
    CAGGACTCTC GCATCAAACA GTTGGAGACA GGAGCATTAC AGATCCGATA TGCCAAGCTG
    GGTGATACTG GTCGGTACAC CTGCGTTGCT TCAACCCCAA GTGGCGAAGC TACATGGAGT
    GCCTACATAG AGGTCCAAGA ATTTGGAGTC CCAGTGCAGC CACCAAGACC CACTGACCCA
    AACTTGATTC CTAGTGCTCC ATCAAAACCT GAGGTTACAG ATGTCAGCAG AAATACAGTA
    ACATTGTCAT GGCAACCTAA TTTGAATTCA GGTGCAACGC CAACCTCTTA CATAATTGAG
    GCCTTCAGCC ATGCATCTGG TAGCAGCTGG CAAACTGTAG CTGAAAATGT GAAAACAGAG
    AGTTTTGCAG TTAAAGGGCT AAAACCTAGT GCAATTTATC TATTCCTTGT GAGGGCAGCT
    AATGCATATG GACTAAGTGA CCCAAGCCAA ATATCTGATC CAGTGAAAAC TCAAGATGTT
    CCACCAACAA GTCAAGGTGT GGATCACAAG CAGGTGCAGA GAGAACTAGG CGATGTGGTA
    CTACATCTGC ACAACCCTAC TATCCTTTCT TCCTCCTCAA TTGAAGTTCA TTGGACAGTG
    GACCAGCAAT CCCAATATAT TCAAGGATAT AAAATCCTAT ACCGGCCAAC ACCAGCGTCC
    TATGGAGAGT CAGAATGGTT AATTTTTGAA GTGAGGACTC CAACCAAAAA CAGTGTTATC
    ATTCCAGAGC TGAAGAAAGG TGTAAACTAT GAAATCAAGG CCCGCCCTTT TTTTAATGAG
    TTTCAAGGCG CAGATAGTGA AGTGAAATTT GCAAAAACCC TAGAAGAAGC GCCCAGTGCT
    CCACCTCAGA GTGTTTCAGT AACTAAAAAT GATGGGAATG GAACTGCAAT TGTAGTCACC
    TGGCAGCCAC CCCCCGAGGA TAACCAGAAC GGAATGGTTC AAGAATACAA GGTTTGGTGC
    CTTGGCAATG AAAGTCGATA CCATATAAAC AGGACAGTAG ATGGTTCCAC CTTTTCAGTA
    GTCATCCCTT CCCTAGTACC AGGTATTCGA TACAGCGTGG AAGTGGCAGC AAGTACTGGA
    GCAGGACCTG GAGTGAAAAG TGATCCCCAG TTTATCCAGT TAGATTCGCA TGGAAATCCT
    GTGTCTTCAG AAGATCAAGT CAGTTTAGCC CAGCAGATCT CTGACGTAGT AAAACAGCCT
    GCCTTTATTG CTGGAATTGG TGCTGCTTGT TGGATTATCC TCATGGTCTT CAGCATCTGG
    TTGTATCGCC ACAGAAAGAA GCGAAATGGA CTCACTAGTA CTTACGCTGG TATCAGAAAA
    GTCCCGTCTT TTACCTTCAC ACCAACAGTA ACCTATCAGA GAGGAGGAGA TGCCGTAAGC
    AGTGGAGGCA GGCCAGGACT CCTAAACATC AGTGAACCAG CCACACAGCC CTGGCTAGCA
    GATACTTGGC CTAACACAGG GAACAACCAC AATGACTGCT CCATCAACTG CTGCACCTCT
    GGCAATGGAA ACAGTGATGG CAACCTTACG ACATACAGTC GACCAGCCGA TTGTATAGCA
    AATTACAACA ATCAGCTGGA CAACAAGCAG ACAAACCTGA TGTTGCCTGA GTCAACTGTA
    TATGGTGACG TGGACCTAAG CAACAAAATC AATGAGATGA AAACCTTCAA TAGTCCAAAT
    CTAAAGGATG GAAGATTTGT CAGCCAACCT GGGCAGCCCA CTCCTTATGC CACCACTCAA
    CTCATCCAGT CAAATATCAG CAATAATGTT AACAATGGCA GCGGGGATAC GAATGAGAAA
    CACTGGAAAC CCTCTGTTCA GCAAAAGCAA GAGGTTGCAC CCATACAGTA CAACATTATG
    GAGCAAAACA AATTAAACAA AGATTACCGA GTAAATGACA GCATTGCTCC AACAATTCCA
    TACAACCAGT CATATGACCA GAATACAGGT GGATCCTACA ACAGCTCAGA TAGAGGCAGT
    AGCACATCTG GGAGTCAAGG GCACAAGAAG GGTGCTCGGA CTCCAAAGGC TCCCAAGCAA
    GCTGGCATGA ACTGGGCAGA TCTTCTTCCA CCCCCTCCAG CACATCCACC TCCCCATAGC
    AATAGTGAAG ATTACAGTCT TTCAGTGGAT GAAAGACGTC AGCCTGTGAG CCCTCCACCC
    CCTCCAAGGC CTATCTCTCC ACCACATACC TATGGATATA TCTCAGGGCC TTTAGTCTCT
    GACATGGATA CTGATGCACC AGAAGAGGAA GAAGATGAGG CAGATATGGA GGTTGCTAAG
    ATGCAAAACA GAAGACTCCT TTTGCGTGGC CTTGAGCAGA CACCTGCTTC AAGTGTTGGT
    GATTTGGAGA GCTCTGTAAC AGGGTCCATG ATCAATGGCT GGGGTTCAGC TTCCGAAGAA
    GATAACATCT CAAGTGGGCG GTCTAGTGTT AGCTCTTCAG ATGGATCATT TTTTACTGAT
    GCAGATTTTG CACAGGCTGT TGCAGCAGCT GCAGAATATG CTGGCTTGAA AGTAGCCAGA
    CGTCAAATGC AGGATGCAGC AGGAGGCCGT AGACATTTTC ATGCATCACA TTGTCCCAGA
    CCTACCAGCC CTGTATCTAC TGACAGCAAC ATGAGTGCTG TTGTAATACA GAAGGTCCGA
    CCTGCCAAAA AGCAAAAACA CCAGCCAGGA CATCTGCGCA GAGAAGTCTA CACAGATGAC
    TTGCCACCCC CTCCCGTGCC ACCTCCCGCT ATAAAATCAC CAACCGCCCA GTCCAAATCT
    CAGCTGGAGG TGCGACCTGT CATGTTGCCA AAACTGGCTT CTATAGAACC GAGGACAGAC
    AGATCTGCGG AAAGAAAAGG AGTGAGCTAC AAGGGGAGGG ACGGGGTGGA TGGGAGACAG
    CATTCAGACA TGCGGACAAA TTCAGGAGAG CGGAGAGAAT CACAGGAACA GCAAGGCGAC
    GGGAAACTGC GAGGAAGTAA AGCACCAAAA CGAGAAGGCA CACCAGCAAA AACTCATCTT
    CTTCAAGAGG ACATCCTGCC ATATTCTAGA CCAACGTTTC CAACGTCAAA TAATCCCAGA
    GATCCCAGCT CCTCCAGCTC TATGTCTTCG AGAGGATCAG GAGGGAGACA GAGGGCAGAT
    CAAGCAAATA TAGCCCGAAG AAATGTTGCA GAAATGCAAG TACTGGGAGC ATATGAACAA
    GGAGAAGAAG AAGAAGATGA GATGGAGGAA ACAGAAAGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.