unc-13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-13
Entrez Gene ID	43841
Full Name	CG2999 gene product from transcript CG2999-RD
Synonyms	CG2999,DUNC-13,Dmel\CG2999,Dunc-13,Dunc13,Munc-13,Munc-13/UNC-13,UNC-13,UNC-13-B,Unc-13,dUNC-13,dUnc-13,drunc13,dunc-13,l(4)ry16,unc
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 4 Map Location: 102E5-102F1

mRNA and Protein(s)

mRNA	Protein	Name
NM_001272146.1	NP_001259075.1	unc-13, isoform F
NM_001258498.2	NP_001245427.1	unc-13, isoform E
NM_001297788.1	NP_001284717.1	unc-13, isoform G
NM_143692.4	NP_651949.2	unc-13, isoform A
NM_166794.3	NP_726615.1	unc-13, isoform B
NM_166793.4	NP_726614.2	unc-13, isoform C
NM_001258497.2	NP_001245426.1	unc-13, isoform D

Pathways from BioSystems

Homologies

Bos taurus (cattle)	UNC13A	XP_002688619.2
Rattus norvegicus (Norway rat)	Unc13a	NP_074052.1
Danio rerio (zebrafish)	si:dkey-110c1.7	NP_001038630.1
Xenopus tropicalis (tropical clawed frog)	LOC100492289	XP_002938873.2
Homo sapiens (human)	UNC13A	NP_001073890.2
Gallus gallus (chicken)	LOC430487	XP_428042.4
Canis lupus familiaris (dog)	UNC13A	XP_005633256.1
Caenorhabditis elegans (roundworm)	unc-13	NP_001021874.1
Mus musculus (house mouse)	Unc13a	NP_001025044.2
Drosophila melanogaster (fruit fly)	unc-13	NP_651949.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission
Title: Stable Positioning of Unc13 Restricts Synaptic Vesicle Fusion to Defined Release Sites to Promote Synchronous Neurotransmission.

identified isoform-specific Unc13-Active Zone scaffold interactions regulating Synaptic Vesicle-Ca2+-channel topology whose developmental tightening optimizes synaptic transmission.
Title: Active zone scaffolds differentially accumulate Unc13 isoforms to tune Ca(2+) channel-vesicle coupling.

Munc13-1 C1 has binding site(s) for alcohols and Munc13-1 activity is sufficient to restore normal self-administration to Drosophila mutants
Title: The pre-synaptic Munc13-1 binds alcohol and modulates alcohol self-administration in Drosophila.

The following unc-13 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-13 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa00201	NM_001258497.2 Latest version!	Drosophila melanogaster unc-13, transcript variant D (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00201	NM_143692.4 Latest version!	Drosophila melanogaster unc-13, transcript variant A (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00202	NM_166793.4 Latest version!	Drosophila melanogaster unc-13, transcript variant C (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00203	NM_166794.3 Latest version!	Drosophila melanogaster unc-13, transcript variant B (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00317	NM_001272146.1 Latest version!	Drosophila melanogaster unc-13, transcript variant F (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa00318	NM_001258498.2 Latest version!	Drosophila melanogaster unc-13, transcript variant E (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22196	NM_001297788.1 Latest version!	Drosophila melanogaster unc-13, transcript variant G (unc-13), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-13 ( NM_143692.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651949.2 Drosophila melanogaster unc-13, transcript variant A (unc-13), mRNA.

Price & Availability↑

CloneID	OFa00201
Clone ID Related Accession (Same CDS sequence)	NM_143692.4 , NM_001258497.2
Accession Version	NM_143692.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_143692.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGAC AATGGCCGCT 
ACAAAACGAA ATGCTGGACT CACATCTGCT GTTCCACGTG CAACGCTGAA TGACGAAGAT 
CTGAAAATGC ACGTGTACAA AAAAGCTCTA CAAGCCCTGA TATATCCTAT ATCCTCAACA 
ACGCCCCACA ATTTTTTGTT ATGGACTGCT ACGTCACCAA CGTACTGCTA CGAGTGTGAG 
GGTCTTTTGT GGGGGATAGC ACGACAAGGG GTCCGTTGCA CTGAGTGCGG TGTAAAGTGT 
CATGAAAAAT GTAAGGACCT TCTGAATGCG GACTGCCTGC AGCGTGCTGC CGAAAAAAGC 
TCAAAGCACG GTGCGGAAGA CAAGGCTAAC TCGATTATTA CAGCGATGAA AGATCGTATG 
AAGCAACGTG AACGAGAGAA GCCTGAAATT TTTGAGTTGA TTAGGATGAC ATTCGGTGTG 
GATCCCGATA CACACATAGA TTCATTAGAG CAGGCTGAGC ACGCAACAGT TGAGGGAACA 
TCTAAGTGGT CATGCAAACT AACAATTACA GTGATTTGTG CTCAAGGCCT GATAGCGAAG 
GATAAAAGCG GTACCAGTGA TCCATATGTG ACGGTGCAAG TTAGCAAGGT GAAAAAACGA 
ACGCGAACAA TGCCGCAGGA ACTTAATCCT GTGTGGAACG AGAAGTTTCA CTTCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651949.2
CDS	591..9206
Translation	MTHYVRHDYFHNTQNGALSSDTSRISYSQISYETQPSREYFSESYALSNQGPEECSRVSHLNSDTVLTTVDNSNNSYGYDYLECYGANIQCDPEEDSVDNWNENTSVVADQYGLGHNNLNCTSSKLLPKLPNIENGRGSSNACAPQMDVKFNTKGMCIKIDHSYGVCMAKAHDFVGRLSPSDYQNILGNNLNGYAGCAYSSTFLDNAMSSAPLRVLPQSPRCSSYLGRNIIGFNADAAQRDGRGFDTDQTDAMGSESSTYEVYEKMQRPYTSMLPLDYSDYQEGCYNTDNLSTYSDTPPSNNTQLKRQMQRKISLMMAMTTASVIASGEIRVPVHSKQSKKSTEIQTDSIIGNTISTNAAARDLDRCLATESCEVIVDTRDSGSVTSFPSSAVTAITKTRKLPKVLPTPLCKSSRHPITIATDALSSSYTSDPLPEKSHRPKQLPKLPISLPQSNDRASLNSNWATPPAPDALPFNSFDHKSASSPTPTTTITKDTETTSYLVETDFIGARHNALYQYDSKEPNIVFSDKSVEAEHSPTWTPLSPIQSKQSPCPPVALPSNIMQNVSLTCHLPEIEATRSDIEREPESSSIEPILEIEKLADPYSGPGSALFNISEYLKPYTLNKPILSEEKKNHIANAASTSTTTPLNITSDDEFSSYSNKWTSTCVNFQPLDVESSLNISLKVNAGTNQAELLMTPLKSSTPLFISSNGTSDNFNLRKSSPPDSAFTTTVNVNSFETVLVSGSQTASPSPSNLKSPPSIAPLLSYSDYMKQFELPELPQPIMDLSENDTATQSDSFNVINNTLTNADNLNSYNQMDVESKSSLQLPSYSSESFDPCSVPSFSIKNKEYKIVEKLDSLSNVESVESPKTLVSPVNPLNCSKLLPGTESIVSNDVAFDDTFYDSFNVDIKELTAFVDHVAPEDGLYNFPNDKTSVEFSFDKTEDTIDMNQNLSSGECGYYKPSQAQQKASVVASAASSVLDGISKGLKGGLDGVFSGVSSTVDVTQSNPSSKRGFSFNLASKIVPSVGGLLTSTSSTSIKQTGSETNPTLILISPENVSSRNSNYIPTTSPSCTQKNGEENLYSATVHNKSTKSNSYYNEVGEISSTLVRNVCDSYDNSYDEMILTNEMVNIGMLDSESEFGLIENSYSYQVPDNEQIDSVNSYNNKTQNVTNNGIEKANTKNKPVPLHDPPTKKASTVGMFGSILGKAAAAVQSATQAVNQSASSVASVVAQKPTIVPRTNNVLLLSSVCSPNEIKRNSSSVEFDSEYGYQMPDVESLSSHYANTGGDYDNSNMKIHEFGTYADDRPYADYHTNGNQSQFKEEAVIPGEPEVINTNILPIGPQATGKKLPTVNGKSALLIKQMPTEVYDDESDTDELDVSPSTGKVPSYSIYSEQEDYYMDLQQTTPSIQPNGFYEQVNNGYDYREDYFNEEDEYKYLEQQREQEEHNQPKNKKYLKQAKISKIQPPSLDFIDVGQDDDFIYDNYHSEDDSGNYLEGSSSGSVGPIEGSIIKVDSNIEASFASLNKKSDSFTPTNDSLQKHDTVIGESTTKLTRLRTEKMCPDVDEEDENLSDHVSDLTDLSKLISQKKKTLLRGETEEVVGGHMQVLRQTEITARQRWHWAYNKIIMQLNNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRMTFGVDPDTHIDSLEQAEHATVEGTSKWSCKLTITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_143692.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant A (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143692.4

ORF Insert Sequence:

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGAC AATGGCCGCT 
ACAAAACGAA ATGCTGGACT CACATCTGCT GTTCCACGTG CAACGCTGAA TGACGAAGAT 
CTGAAAATGC ACGTGTACAA AAAAGCTCTA CAAGCCCTGA TATATCCTAT ATCCTCAACA 
ACGCCCCACA ATTTTTTGTT ATGGACTGCT ACGTCACCAA CGTACTGCTA CGAGTGTGAG 
GGTCTTTTGT GGGGGATAGC ACGACAAGGG GTCCGTTGCA CTGAGTGCGG TGTAAAGTGT 
CATGAAAAAT GTAAGGACCT TCTGAATGCG GACTGCCTGC AGCGTGCTGC CGAAAAAAGC 
TCAAAGCACG GTGCGGAAGA CAAGGCTAAC TCGATTATTA CAGCGATGAA AGATCGTATG 
AAGCAACGTG AACGAGAGAA GCCTGAAATT TTTGAGTTGA TTAGGATGAC ATTCGGTGTG 
GATCCCGATA CACACATAGA TTCATTAGAG CAGGCTGAGC ACGCAACAGT TGAGGGAACA 
TCTAAGTGGT CATGCAAACT AACAATTACA GTGATTTGTG CTCAAGGCCT GATAGCGAAG 
GATAAAAGCG GTACCAGTGA TCCATATGTG ACGGTGCAAG TTAGCAAGGT GAAAAAACGA 
ACGCGAACAA TGCCGCAGGA ACTTAATCCT GTGTGGAACG AGAAGTTTCA CTTCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-13 ( NM_001258497.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245426.1 Drosophila melanogaster unc-13, transcript variant D (unc-13), mRNA.

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CloneID	OFa00201
Clone ID Related Accession (Same CDS sequence)	NM_143692.4 , NM_001258497.2
Accession Version	NM_001258497.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001258497.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGAC AATGGCCGCT 
ACAAAACGAA ATGCTGGACT CACATCTGCT GTTCCACGTG CAACGCTGAA TGACGAAGAT 
CTGAAAATGC ACGTGTACAA AAAAGCTCTA CAAGCCCTGA TATATCCTAT ATCCTCAACA 
ACGCCCCACA ATTTTTTGTT ATGGACTGCT ACGTCACCAA CGTACTGCTA CGAGTGTGAG 
GGTCTTTTGT GGGGGATAGC ACGACAAGGG GTCCGTTGCA CTGAGTGCGG TGTAAAGTGT 
CATGAAAAAT GTAAGGACCT TCTGAATGCG GACTGCCTGC AGCGTGCTGC CGAAAAAAGC 
TCAAAGCACG GTGCGGAAGA CAAGGCTAAC TCGATTATTA CAGCGATGAA AGATCGTATG 
AAGCAACGTG AACGAGAGAA GCCTGAAATT TTTGAGTTGA TTAGGATGAC ATTCGGTGTG 
GATCCCGATA CACACATAGA TTCATTAGAG CAGGCTGAGC ACGCAACAGT TGAGGGAACA 
TCTAAGTGGT CATGCAAACT AACAATTACA GTGATTTGTG CTCAAGGCCT GATAGCGAAG 
GATAAAAGCG GTACCAGTGA TCCATATGTG ACGGTGCAAG TTAGCAAGGT GAAAAAACGA 
ACGCGAACAA TGCCGCAGGA ACTTAATCCT GTGTGGAACG AGAAGTTTCA CTTCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245426.1
CDS	524..9139
Translation	MTHYVRHDYFHNTQNGALSSDTSRISYSQISYETQPSREYFSESYALSNQGPEECSRVSHLNSDTVLTTVDNSNNSYGYDYLECYGANIQCDPEEDSVDNWNENTSVVADQYGLGHNNLNCTSSKLLPKLPNIENGRGSSNACAPQMDVKFNTKGMCIKIDHSYGVCMAKAHDFVGRLSPSDYQNILGNNLNGYAGCAYSSTFLDNAMSSAPLRVLPQSPRCSSYLGRNIIGFNADAAQRDGRGFDTDQTDAMGSESSTYEVYEKMQRPYTSMLPLDYSDYQEGCYNTDNLSTYSDTPPSNNTQLKRQMQRKISLMMAMTTASVIASGEIRVPVHSKQSKKSTEIQTDSIIGNTISTNAAARDLDRCLATESCEVIVDTRDSGSVTSFPSSAVTAITKTRKLPKVLPTPLCKSSRHPITIATDALSSSYTSDPLPEKSHRPKQLPKLPISLPQSNDRASLNSNWATPPAPDALPFNSFDHKSASSPTPTTTITKDTETTSYLVETDFIGARHNALYQYDSKEPNIVFSDKSVEAEHSPTWTPLSPIQSKQSPCPPVALPSNIMQNVSLTCHLPEIEATRSDIEREPESSSIEPILEIEKLADPYSGPGSALFNISEYLKPYTLNKPILSEEKKNHIANAASTSTTTPLNITSDDEFSSYSNKWTSTCVNFQPLDVESSLNISLKVNAGTNQAELLMTPLKSSTPLFISSNGTSDNFNLRKSSPPDSAFTTTVNVNSFETVLVSGSQTASPSPSNLKSPPSIAPLLSYSDYMKQFELPELPQPIMDLSENDTATQSDSFNVINNTLTNADNLNSYNQMDVESKSSLQLPSYSSESFDPCSVPSFSIKNKEYKIVEKLDSLSNVESVESPKTLVSPVNPLNCSKLLPGTESIVSNDVAFDDTFYDSFNVDIKELTAFVDHVAPEDGLYNFPNDKTSVEFSFDKTEDTIDMNQNLSSGECGYYKPSQAQQKASVVASAASSVLDGISKGLKGGLDGVFSGVSSTVDVTQSNPSSKRGFSFNLASKIVPSVGGLLTSTSSTSIKQTGSETNPTLILISPENVSSRNSNYIPTTSPSCTQKNGEENLYSATVHNKSTKSNSYYNEVGEISSTLVRNVCDSYDNSYDEMILTNEMVNIGMLDSESEFGLIENSYSYQVPDNEQIDSVNSYNNKTQNVTNNGIEKANTKNKPVPLHDPPTKKASTVGMFGSILGKAAAAVQSATQAVNQSASSVASVVAQKPTIVPRTNNVLLLSSVCSPNEIKRNSSSVEFDSEYGYQMPDVESLSSHYANTGGDYDNSNMKIHEFGTYADDRPYADYHTNGNQSQFKEEAVIPGEPEVINTNILPIGPQATGKKLPTVNGKSALLIKQMPTEVYDDESDTDELDVSPSTGKVPSYSIYSEQEDYYMDLQQTTPSIQPNGFYEQVNNGYDYREDYFNEEDEYKYLEQQREQEEHNQPKNKKYLKQAKISKIQPPSLDFIDVGQDDDFIYDNYHSEDDSGNYLEGSSSGSVGPIEGSIIKVDSNIEASFASLNKKSDSFTPTNDSLQKHDTVIGESTTKLTRLRTEKMCPDVDEEDENLSDHVSDLTDLSKLISQKKKTLLRGETEEVVGGHMQVLRQTEITARQRWHWAYNKIIMQLNNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRMTFGVDPDTHIDSLEQAEHATVEGTSKWSCKLTITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_001258497.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant D (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258497.2

ORF Insert Sequence:

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGAC AATGGCCGCT 
ACAAAACGAA ATGCTGGACT CACATCTGCT GTTCCACGTG CAACGCTGAA TGACGAAGAT 
CTGAAAATGC ACGTGTACAA AAAAGCTCTA CAAGCCCTGA TATATCCTAT ATCCTCAACA 
ACGCCCCACA ATTTTTTGTT ATGGACTGCT ACGTCACCAA CGTACTGCTA CGAGTGTGAG 
GGTCTTTTGT GGGGGATAGC ACGACAAGGG GTCCGTTGCA CTGAGTGCGG TGTAAAGTGT 
CATGAAAAAT GTAAGGACCT TCTGAATGCG GACTGCCTGC AGCGTGCTGC CGAAAAAAGC 
TCAAAGCACG GTGCGGAAGA CAAGGCTAAC TCGATTATTA CAGCGATGAA AGATCGTATG 
AAGCAACGTG AACGAGAGAA GCCTGAAATT TTTGAGTTGA TTAGGATGAC ATTCGGTGTG 
GATCCCGATA CACACATAGA TTCATTAGAG CAGGCTGAGC ACGCAACAGT TGAGGGAACA 
TCTAAGTGGT CATGCAAACT AACAATTACA GTGATTTGTG CTCAAGGCCT GATAGCGAAG 
GATAAAAGCG GTACCAGTGA TCCATATGTG ACGGTGCAAG TTAGCAAGGT GAAAAAACGA 
ACGCGAACAA TGCCGCAGGA ACTTAATCCT GTGTGGAACG AGAAGTTTCA CTTCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-13 ( NM_166793.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726614.2 Drosophila melanogaster unc-13, transcript variant C (unc-13), mRNA.

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CloneID	OFa00202
Clone ID Related Accession (Same CDS sequence)	NM_166793.4
Accession Version	NM_166793.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166793.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGGT TCTCAAGACA 
ATGGCCGCTA CAAAACGAAA TGCTGGACTC ACATCTGCTG TTCCACGTGC AACGCTGAAT 
GACGAAGATC TGAAAATGCA CGTGTACAAA AAAGCTCTAC AAGCCCTGAT ATATCCTATA 
TCCTCAACAA CGCCCCACAA TTTTTTGTTA TGGACTGCTA CGTCACCAAC GTACTGCTAC 
GAGTGTGAGG GTCTTTTGTG GGGGATAGCA CGACAAGGGG TCCGTTGCAC TGAGTGCGGT 
GTAAAGTGTC ATGAAAAATG TAAGGACCTT CTGAATGCGG ACTGCCTGCA GCGTGCTGCC 
GAAAAAAGCT CAAAGCACGG TGCGGAAGAC AAGGCTAACT CGATTATTAC AGCGATGAAA 
GATCGTATGA AGCAACGTGA ACGAGAGAAG CCTGAAATTT TTGAGTTGAT TAGAGCCGTG 
TTCAGCGTTG AGGAGAAGAG TCATGCCGGT CATATGAAGG CTGTGAAGCA AAGCGTCCTG 
GATGGTACAA GCAAGTGGTC GGCCAAAATA GCAATCACAG TGATTTGTGC TCAAGGCCTG 
ATAGCGAAGG ATAAAAGCGG TACCAGTGAT CCATATGTGA CGGTGCAAGT TAGCAAGGTG 
AAAAAACGAA CGCGAACAAT GCCGCAGGAA CTTAATCCTG TGTGGAACGA GAAGTTTCAC 
TTCGAGTGCC ACAACTCCTC CGACCGCATT AAGGTTCGTG TTTGGGACGA AGACAATGAC 
CTAAAGTCGA AACTGCGCCA GAAGTTAACT CGAGAGTCGG ACGACTTTTT GGGACAAACA 
ATTATCGAGG TTCGTACTCT TTCCGGTGAA ATGGATGTAT GGTACAACCT GGAAAAGCGC 
ACAGATAAAT CGGCCGTATC AGGCGCGATA CGGCTTCACA TATCTGTTGA AATAAAGGGC 
GAGGAAAAAG TAGCTCCTTA CCACGTACAA TATACCTGTT TACACGAGAA CCTCTTCCAT 
TATCTTTGCG AAGAAAATAC CGGAATGGTC AAGTTGCCAA CTCAAAAAGG GGACGATGCT 
TGGAAGTTGT ATTTTGATGA AATACCCGAA GAGATTGTCG ACGAGTTTTC GATGCGTTAT 
GGAATTGAGA ATATTTATCA AGCTATGACA CATTTCCACT GCTTGTCTGC TAAATACCTA 
TGTCCTGGGG TACCAGCTGT AATGAGTACC CTTTTGGCAA ACATAAATGC TTATTATGCT 
CATACGACTG CTTCGTCAGC TGTATCGGCC AGTGATAGAT TTGCTGCCAG CAATTTTGGG 
AAAGAAAAAT TTGTTAAGTT GCTGGACCAG TTGCATAACT CACTGAGGAT CGATTTGTCA 
ATGTACAGAA ATAACTTTCC TGCATCGAGT CCAGAAAAGC TAATGGATCT AAAATCTACA 
GTTGATTTAT TGACCAGCAT AACATTTTTC CGCATGAAGG TACAAGAGCT ATCTAGCCCG 
CCGAGAGCAA GTACAGTAGT CAAGGATTGT GTTAAAGCAT GCCTTAGATC CACTTATCAA 
TTCTTATTTG AAAATTGTTA TGAACTGTAC AACAGAGAAT TTCAGGTTGA TCCCAACGAA 
GCAAAACGTG CACCAGATGA TCATGAACCC AAGCTTGATA GCGTCGACTT TTGGCATAAG 
CTAATAGCTT TAATCGTATC GGTAATAGAT GAAGACAAAA ACAGTTACGG AACCGTTTTA 
AATCAATTTC CGCAAGAGCT AAATATTGGA CAGTTATCAG CATCTTCAAT GTGGCATCTC 
TTTGCTGTGG ACATGAAGTA CGCGCTCGAG GAGCATGAGC AACATCGTCT TTGCAAGTCA 
TCGGCTTATA TGAATCTTCA CTTTCGAGTT AAGTGGCTAT ATAGTAACTA CGTCAAAGAG 
GTGCCTCCCT ACAAAGGAGC GGTTCCCGAT TACCCAGCAT GGTTCGAGCC TTTTGTGATG 
CAGTGGCTAA ATGAAAATGA CGACGTGTCA TTGGAATACC TTCATGGCGC ATTTAAGAGG 
GATAAAAAAG ACGGGTTTCA AAAGAGCAGC GAGCACGCGC TCTTTTCAAA TTCTGTAGTC 
GACGTTTTTA CGCAATTGAC GCAGTGTTTC GATGTTGTTA GCAAACTCGA GTGTCCTGAT 
CCAGAAATTT GGAAACGTTA CATGAGACGT TTTGCTAAAA CAATCGTAAA AGTGCTAATA 
GCCTATGCGG ATATTGTGAA ACTTGAGTTT CCAGAGCATA TGAAGGATGA AAGAATTGCT 
TGCATACTTA TGAACAACAT TCAGCAACTA AGGGTGCAGC TAGAAAAAAT GTTTGAGTCT 
ATGGGTGGGG ATAAGCTAGA GGAGGATGCA GCCAATATTT TGAAAGAACT TCAGCAAAAT 
CTTAACTCTG CGTTGGACGA CTTAGCTTCA CAATTCGCTA TCAGTTTGGA GCCACGAATA 
ACTCAATCAG TGCGAGAACT TGGAGACATG CTGCTTTCTA TTAAAGGAGG CAGTGGAACC 
CTTGCTGCTG GTAATCTTGC AGCTCAAAGG AACGCGGTCG CCGTTGAGGC CGATGAAGTT 
TTAAGGCCGC TTATGGATTT ACTTGATGGC TCTTTAACTC TCTATGCGCA ATCATGTGAA 
AAAACCGTGT TGAAGAGGTT ACTAAAGGAG TTATGGAAGA TCGTAATGCG AATATTAGAG 
AAAACAATTG TTTTACCACC TATGACTGAT AAAACTATGA TGTTTAAACA CCTTACTGAC 
AATGCAAAAA ACTTGGCATC GAATGCAAAA ATTGAAGACA TGGGTCGTTT GTTCAAGTCA 
CATATGGCAG GAAAGCAGGA TGTTAAGAGT GCTCTGAGCG GGGTAATGGA CATATCAAAA 
GAAGTAGAAA AAAACTTGTC ACCCAAGCAA TGCGCAGTGC TTGATGTTGC CTTAGATACA 
ATAAAACAAT ACTTTCACGC GGGTGGCAAT GGACTGAAAA AGACCTTTCT CGAAAAGTCG 
CCCGAGCTTC AGAGTTTACG TTACGCACTC AGTTTATATA CACAAATGAC CGACACACTA 
ATCAAAACCT TTATATCTAG CCAAGTTCAC GAGGTCGATC TAGAAAACTC AGAGGAAAGT 
GTTGGGGAAA TATCCGTGCA AATAGACTTA TTTTCCCATC CCGGCACCGG TGAACATAAA 
GTAAACGTGA AAGTTGTTGC TGCTAATGAC CTTAAATGGC AAATACCATC GGGTATGTTC 
AGACCATTTG TTGACATTAA TTTAATAGGA CCGCACCTGC AAGAAAAAAA AAGGAAGTTC 
GCTACAAAGT CGAAGTCTAA CAACTGGTCG CCTAAATACA ACGAATCTTT CAGCTTCACC 
ATTGGAAATG AGGAGCAGCT GGACTTCTTT GAATTGCACA TTTGCGTTAA GGACTATTGT 
TTTGCTCGTG ATGACCGACT TGTGGGCGTG GCTGTAATTC CATTAAAAGA TATATCGGAA 
AAGGGATCGG TCGCATGCTG GTTACCCTTA ATGAGACGAA TTGAAATGGA CGAAACGGGC 
TGGACCATTT TAAGAATTTT GTCACAGCGA AATAATGATG AAGTGGCCAA GGAATTTGTC 
AAGCTTAAAT CGGAAATACG GCAGGAGCCA ACCATGGGTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726614.2
CDS	591..9215
Translation	MTHYVRHDYFHNTQNGALSSDTSRISYSQISYETQPSREYFSESYALSNQGPEECSRVSHLNSDTVLTTVDNSNNSYGYDYLECYGANIQCDPEEDSVDNWNENTSVVADQYGLGHNNLNCTSSKLLPKLPNIENGRGSSNACAPQMDVKFNTKGMCIKIDHSYGVCMAKAHDFVGRLSPSDYQNILGNNLNGYAGCAYSSTFLDNAMSSAPLRVLPQSPRCSSYLGRNIIGFNADAAQRDGRGFDTDQTDAMGSESSTYEVYEKMQRPYTSMLPLDYSDYQEGCYNTDNLSTYSDTPPSNNTQLKRQMQRKISLMMAMTTASVIASGEIRVPVHSKQSKKSTEIQTDSIIGNTISTNAAARDLDRCLATESCEVIVDTRDSGSVTSFPSSAVTAITKTRKLPKVLPTPLCKSSRHPITIATDALSSSYTSDPLPEKSHRPKQLPKLPISLPQSNDRASLNSNWATPPAPDALPFNSFDHKSASSPTPTTTITKDTETTSYLVETDFIGARHNALYQYDSKEPNIVFSDKSVEAEHSPTWTPLSPIQSKQSPCPPVALPSNIMQNVSLTCHLPEIEATRSDIEREPESSSIEPILEIEKLADPYSGPGSALFNISEYLKPYTLNKPILSEEKKNHIANAASTSTTTPLNITSDDEFSSYSNKWTSTCVNFQPLDVESSLNISLKVNAGTNQAELLMTPLKSSTPLFISSNGTSDNFNLRKSSPPDSAFTTTVNVNSFETVLVSGSQTASPSPSNLKSPPSIAPLLSYSDYMKQFELPELPQPIMDLSENDTATQSDSFNVINNTLTNADNLNSYNQMDVESKSSLQLPSYSSESFDPCSVPSFSIKNKEYKIVEKLDSLSNVESVESPKTLVSPVNPLNCSKLLPGTESIVSNDVAFDDTFYDSFNVDIKELTAFVDHVAPEDGLYNFPNDKTSVEFSFDKTEDTIDMNQNLSSGECGYYKPSQAQQKASVVASAASSVLDGISKGLKGGLDGVFSGVSSTVDVTQSNPSSKRGFSFNLASKIVPSVGGLLTSTSSTSIKQTGSETNPTLILISPENVSSRNSNYIPTTSPSCTQKNGEENLYSATVHNKSTKSNSYYNEVGEISSTLVRNVCDSYDNSYDEMILTNEMVNIGMLDSESEFGLIENSYSYQVPDNEQIDSVNSYNNKTQNVTNNGIEKANTKNKPVPLHDPPTKKASTVGMFGSILGKAAAAVQSATQAVNQSASSVASVVAQKPTIVPRTNNVLLLSSVCSPNEIKRNSSSVEFDSEYGYQMPDVESLSSHYANTGGDYDNSNMKIHEFGTYADDRPYADYHTNGNQSQFKEEAVIPGEPEVINTNILPIGPQATGKKLPTVNGKSALLIKQMPTEVYDDESDTDELDVSPSTGKVPSYSIYSEQEDYYMDLQQTTPSIQPNGFYEQVNNGYDYREDYFNEEDEYKYLEQQREQEEHNQPKNKKYLKQAKISKIQPPSLDFIDVGQDDDFIYDNYHSEDDSGNYLEGSSSGSVGPIEGSIIKVDSNIEASFASLNKKSDSFTPTNDSLQKHDTVIGESTTKLTRLRTEKMCPDVDEEDENLSDHVSDLTDLSKLISQKKKTLLRGETEEVVGGHMQVLRQTEITARQRWHWAYNKIIMQLNNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELVLKTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_166793.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant C (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166793.4

ORF Insert Sequence:

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGGT TCTCAAGACA 
ATGGCCGCTA CAAAACGAAA TGCTGGACTC ACATCTGCTG TTCCACGTGC AACGCTGAAT 
GACGAAGATC TGAAAATGCA CGTGTACAAA AAAGCTCTAC AAGCCCTGAT ATATCCTATA 
TCCTCAACAA CGCCCCACAA TTTTTTGTTA TGGACTGCTA CGTCACCAAC GTACTGCTAC 
GAGTGTGAGG GTCTTTTGTG GGGGATAGCA CGACAAGGGG TCCGTTGCAC TGAGTGCGGT 
GTAAAGTGTC ATGAAAAATG TAAGGACCTT CTGAATGCGG ACTGCCTGCA GCGTGCTGCC 
GAAAAAAGCT CAAAGCACGG TGCGGAAGAC AAGGCTAACT CGATTATTAC AGCGATGAAA 
GATCGTATGA AGCAACGTGA ACGAGAGAAG CCTGAAATTT TTGAGTTGAT TAGAGCCGTG 
TTCAGCGTTG AGGAGAAGAG TCATGCCGGT CATATGAAGG CTGTGAAGCA AAGCGTCCTG 
GATGGTACAA GCAAGTGGTC GGCCAAAATA GCAATCACAG TGATTTGTGC TCAAGGCCTG 
ATAGCGAAGG ATAAAAGCGG TACCAGTGAT CCATATGTGA CGGTGCAAGT TAGCAAGGTG 
AAAAAACGAA CGCGAACAAT GCCGCAGGAA CTTAATCCTG TGTGGAACGA GAAGTTTCAC 
TTCGAGTGCC ACAACTCCTC CGACCGCATT AAGGTTCGTG TTTGGGACGA AGACAATGAC 
CTAAAGTCGA AACTGCGCCA GAAGTTAACT CGAGAGTCGG ACGACTTTTT GGGACAAACA 
ATTATCGAGG TTCGTACTCT TTCCGGTGAA ATGGATGTAT GGTACAACCT GGAAAAGCGC 
ACAGATAAAT CGGCCGTATC AGGCGCGATA CGGCTTCACA TATCTGTTGA AATAAAGGGC 
GAGGAAAAAG TAGCTCCTTA CCACGTACAA TATACCTGTT TACACGAGAA CCTCTTCCAT 
TATCTTTGCG AAGAAAATAC CGGAATGGTC AAGTTGCCAA CTCAAAAAGG GGACGATGCT 
TGGAAGTTGT ATTTTGATGA AATACCCGAA GAGATTGTCG ACGAGTTTTC GATGCGTTAT 
GGAATTGAGA ATATTTATCA AGCTATGACA CATTTCCACT GCTTGTCTGC TAAATACCTA 
TGTCCTGGGG TACCAGCTGT AATGAGTACC CTTTTGGCAA ACATAAATGC TTATTATGCT 
CATACGACTG CTTCGTCAGC TGTATCGGCC AGTGATAGAT TTGCTGCCAG CAATTTTGGG 
AAAGAAAAAT TTGTTAAGTT GCTGGACCAG TTGCATAACT CACTGAGGAT CGATTTGTCA 
ATGTACAGAA ATAACTTTCC TGCATCGAGT CCAGAAAAGC TAATGGATCT AAAATCTACA 
GTTGATTTAT TGACCAGCAT AACATTTTTC CGCATGAAGG TACAAGAGCT ATCTAGCCCG 
CCGAGAGCAA GTACAGTAGT CAAGGATTGT GTTAAAGCAT GCCTTAGATC CACTTATCAA 
TTCTTATTTG AAAATTGTTA TGAACTGTAC AACAGAGAAT TTCAGGTTGA TCCCAACGAA 
GCAAAACGTG CACCAGATGA TCATGAACCC AAGCTTGATA GCGTCGACTT TTGGCATAAG 
CTAATAGCTT TAATCGTATC GGTAATAGAT GAAGACAAAA ACAGTTACGG AACCGTTTTA 
AATCAATTTC CGCAAGAGCT AAATATTGGA CAGTTATCAG CATCTTCAAT GTGGCATCTC 
TTTGCTGTGG ACATGAAGTA CGCGCTCGAG GAGCATGAGC AACATCGTCT TTGCAAGTCA 
TCGGCTTATA TGAATCTTCA CTTTCGAGTT AAGTGGCTAT ATAGTAACTA CGTCAAAGAG 
GTGCCTCCCT ACAAAGGAGC GGTTCCCGAT TACCCAGCAT GGTTCGAGCC TTTTGTGATG 
CAGTGGCTAA ATGAAAATGA CGACGTGTCA TTGGAATACC TTCATGGCGC ATTTAAGAGG 
GATAAAAAAG ACGGGTTTCA AAAGAGCAGC GAGCACGCGC TCTTTTCAAA TTCTGTAGTC 
GACGTTTTTA CGCAATTGAC GCAGTGTTTC GATGTTGTTA GCAAACTCGA GTGTCCTGAT 
CCAGAAATTT GGAAACGTTA CATGAGACGT TTTGCTAAAA CAATCGTAAA AGTGCTAATA 
GCCTATGCGG ATATTGTGAA ACTTGAGTTT CCAGAGCATA TGAAGGATGA AAGAATTGCT 
TGCATACTTA TGAACAACAT TCAGCAACTA AGGGTGCAGC TAGAAAAAAT GTTTGAGTCT 
ATGGGTGGGG ATAAGCTAGA GGAGGATGCA GCCAATATTT TGAAAGAACT TCAGCAAAAT 
CTTAACTCTG CGTTGGACGA CTTAGCTTCA CAATTCGCTA TCAGTTTGGA GCCACGAATA 
ACTCAATCAG TGCGAGAACT TGGAGACATG CTGCTTTCTA TTAAAGGAGG CAGTGGAACC 
CTTGCTGCTG GTAATCTTGC AGCTCAAAGG AACGCGGTCG CCGTTGAGGC CGATGAAGTT 
TTAAGGCCGC TTATGGATTT ACTTGATGGC TCTTTAACTC TCTATGCGCA ATCATGTGAA 
AAAACCGTGT TGAAGAGGTT ACTAAAGGAG TTATGGAAGA TCGTAATGCG AATATTAGAG 
AAAACAATTG TTTTACCACC TATGACTGAT AAAACTATGA TGTTTAAACA CCTTACTGAC 
AATGCAAAAA ACTTGGCATC GAATGCAAAA ATTGAAGACA TGGGTCGTTT GTTCAAGTCA 
CATATGGCAG GAAAGCAGGA TGTTAAGAGT GCTCTGAGCG GGGTAATGGA CATATCAAAA 
GAAGTAGAAA AAAACTTGTC ACCCAAGCAA TGCGCAGTGC TTGATGTTGC CTTAGATACA 
ATAAAACAAT ACTTTCACGC GGGTGGCAAT GGACTGAAAA AGACCTTTCT CGAAAAGTCG 
CCCGAGCTTC AGAGTTTACG TTACGCACTC AGTTTATATA CACAAATGAC CGACACACTA 
ATCAAAACCT TTATATCTAG CCAAGTTCAC GAGGTCGATC TAGAAAACTC AGAGGAAAGT 
GTTGGGGAAA TATCCGTGCA AATAGACTTA TTTTCCCATC CCGGCACCGG TGAACATAAA 
GTAAACGTGA AAGTTGTTGC TGCTAATGAC CTTAAATGGC AAATACCATC GGGTATGTTC 
AGACCATTTG TTGACATTAA TTTAATAGGA CCGCACCTGC AAGAAAAAAA AAGGAAGTTC 
GCTACAAAGT CGAAGTCTAA CAACTGGTCG CCTAAATACA ACGAATCTTT CAGCTTCACC 
ATTGGAAATG AGGAGCAGCT GGACTTCTTT GAATTGCACA TTTGCGTTAA GGACTATTGT 
TTTGCTCGTG ATGACCGACT TGTGGGCGTG GCTGTAATTC CATTAAAAGA TATATCGGAA 
AAGGGATCGG TCGCATGCTG GTTACCCTTA ATGAGACGAA TTGAAATGGA CGAAACGGGC 
TGGACCATTT TAAGAATTTT GTCACAGCGA AATAATGATG AAGTGGCCAA GGAATTTGTC 
AAGCTTAAAT CGGAAATACG GCAGGAGCCA ACCATGGGTA CATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-13 ( NM_166794.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_726615.1 Drosophila melanogaster unc-13, transcript variant B (unc-13), mRNA.

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CloneID	OFa00203
Clone ID Related Accession (Same CDS sequence)	NM_166794.3
Accession Version	NM_166794.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_166794.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAACA CATCTCAGCT GCAAGTGACC GGGGATACGG AAAAAAAAAG TCAGTTGCTT 
AAAAAGGAAC TCAAAATAAA CACCCAGGAA AAGCTTATCT TTGCAGAAAA TGCCCTCAAA 
AGCCAGATTA AAATAAAGGA GCAGTTACGA CTTCAGCAAC AATCAACGAT TTATGCGTCG 
TCACTATTGA GCTCTTCTGC TGCCGGATCA GTAAGAGCTC CGCTCTTAAG CCAGGGGCAC 
CTTAATTCTA TCCAACACAA CATGGATTTT GACCTTGCCA AAGCACAAAT ACCGGAAATG 
CAACCACCGA TGAGTAAGTC GCCCAATGGA TTGGATTTCT CTTACTTAAG TTATCCAAGC 
ATAAATACTA ATGAAAGTAT GATATCGATT AAAAGTGAAC AACAGTTGTG TCAGAGCTAC 
AATTCGGAGC AACACTCTGA CTACATTATC TCCGATTATA TGGATAAAAT TGCAACAAGG 
ATCAGTTTAC TCGAGACAGA GCTGAAGTTT GCTTGGCGTG CATTGGATTT GCTTTCTACG 
GAGTACGGAA AGATTTGGAT TCGTTTGGAA AAGCTTGAAA ACATATCAAT AGAACAACAG 
TCTGTCGTTG GCAACCTTGT GGATCTTATC GGCGCATCAA AAAAAGAACT TCAAAAAGTG 
GATATCGAAA GAATGAAAGT ACCCTTATAT CAAGATGAAG ATCAATTGCT ACCGTTGGAA 
ATGGAAGACA CATTAGATAT AGATATCCAA TCATCTAATC GTGACTTTGA TAAGAATCTT 
ACTTTTGAAA ATCATGAAAA AACTTTTGTA ACAAAACATA CGCAAGCTAC TAAATCGGAA 
GACCTTATGA ATTCCGCTTA CGCAATAGAT TCCCATCCAA ATTTTGAAAA CATTGATTTC 
AATGGCAAAA ACTTAGACAT TGGGATAATA AAATTTGGTT TTGAAAAAGG CTACGAGCCA 
AAACAAAAAG GTAATCAATC GGATTTTGAA GCATACAAGG AATCAAGAAC TAAACTTTAC 
TCAGATTCTG ACCTTATGTT ATATGAGAGG CAGCAGTTTC TAGCAAATAG CGCGCGTGCC 
GAATTAATGA AAGAATTTTT AAATGGACGT CGAGTTTTAA ATGAGATTTC GGCATCCTCG 
GGTGCATTGT CAAAAAGTTC TGAGAAGGAT AAAAATCTTG TAAAGCCTAG AGAACCACTG 
TTAAGTGAGA TAGATGATAC ATACGTGAGA CGGTCAACCT GTGAACTTGT CAGCCCTAGT 
AGTAATAATT TTTTTCAAAT AGCAGCCTCT AAAACAGGCG AGGGTGGAAA TCAGGTTTTA 
CCCATTGAAG ACACTGGAGG AACAGAAAGT CCAGCTAATA TTAATGAAGT AGACGAATCT 
TTTTATAAAA ATCTAAACGA GGCTTATCGA GACAATGAAT TGAGCTCTGA GATATTTAAG 
GTTGATGCAT TGCTACATCA GTCTGAGGCC ACACATGACC ACATCTCATT TATCCGAAAC 
CAGTCAACTT CTTCCCCAGT TTTAAATAAT CAGAGGGTGA ATGCTATTAC AGGTACACAA 
CCTTTACAGA CTGCAAAGGC GTGTCTCGTA ACAGAGCCAA ACCAACATTT GAACAAAGAA 
CCAAGAAAGC ACAGAAAAAA AAAACATCAT ACAAACGAAA TGGATATGAT TAATAAACTA 
AAGTGTATCC TTGCACAAGC ACAATCAACA GAGATTATTA AGAGAGACAT TGAAGAATTG 
GGTTCGAAAC TTTCAGATGC ATGTGTAAAA CAAACTGAAA CGGAAAATAA ATCAGACAAC 
CATATTAGTA CCAGTTATTT AAAAGATGAT ATACGAAAAA TGTTAAACAA CGTAATACAG 
ACTTTATTGG GGGAAATAAA TAAAATTAAA GGGTTACAAT CATTGAGTGC AACGCAATTG 
AGTCAGTTAC AAAATGCCGT ATGGTCGGAA GAAAGGTTTT TTCAAAAAAT TAGTCTGGTC 
GATAAAAAGT TGACTTTGCT GTTACTTAAT CCGTTAACTG TTACTGAAGA ACTGCAAAGG 
CTTTGCATTT CAAACACAAA TGAAAAGTTT GTTCTGGTAA TAAAAAAGTT AAAAAAAAAT 
ATTGACACTC TAAAAAAATT AGTTGGCAGC TCATTGGATG ACTTTAAAAT AGATAATTCA 
CACGCAAATA TGACCACATT TCACAGCCTT AATCCATCCC ATAATTCTAA TGATAGCAGT 
TTTAGTGCAC ATTTATTAAG AAATAACTCC AATCTGGATG AACAGTTGAA AATTCTTGAA 
ACTCAAGAGA TTGAAATACA CCGGAAAAAG AAAATAGACG AAATTGTGAG CGGACTTTCC 
GAAGAAACCT ATACTTTTAA CCCAGATATG GAATATAAAA GCCAACATAG TTGTACCAAC 
TCAACTGATA ATATTATAGG ATTCTCAAAA AATATTTACA ATGAAGATGA ATATATAAAG 
TCTTTAAGAA AGAGTCTAGA ACGCCACAAT AGCATGATCT TTTTACTACA TTTGCAAAAT 
CCTGAAAAAC ACAAAGTGTT GGCTGATATA AATGATGCAC AAATGGATTC AAATCGAGCT 
AGTCTCAGTC CTCCACCACC AGCTCCAACC GACACAGTTT ACTTAAATGA TGCAATTAAT 
CAAATTACAT ATCAACAACG AAATGGAAAA TCAGACTCTG GCCTATCGTC GATGAGCGGC 
TGGTCAGCCA ACTCCCAAGT GTCGGTTGGA CTTCAAAATT ATGATCCTGC ATGTAATAAT 
ATTTTGGAAA ATTGTAGCGA AAGATTGCAA ACATACCATT CATTTCCTTT GGCTGAAAAT 
TCGAACTTTC ACAGTTTCCC ACTCAGTCAT GAACAAGCTC AAACTACTAA TCAAACTGAG 
TTTGTAATAT TAGAAGAAAA TCTAAACTAT ATTTATGAGC TTTCTAAAAA TTTACCTATT 
TGCTCTGCCT ATGAAAATAA ATCTATTTTC GATATGAAAT ATGAAATTTG CGACTCAAAT 
GAAATTGGAA AATTTTCCAC TGTGGACGAA ATGCTGGAAT GGGACCAGCT AAATGAACCG 
GATAAACAAA ACTTTTCTGG GAAACGGTTA AACTCCAACC TTATGCCAGA TCTCTTGACT 
GCTCAAATAC CAAATTCCAT ATCAAAACAC AATAAAAACA CAAGTGTTAA CATTGAATAT 
GTTAGACAAA AAGAAAATAA AGGCATGGAT AGGCGAAGCA TCATAGAACC AAATATTTAT 
AACGGAAAAT CGGAAGATCA AATTTGCAGA CCTTGTTTGA CAGATAAACT CGTATTTTAT 
CCCTCATCGA ACTCTATTAC CGATCACAAT AGCAGCCATG ATTTCAATTG TTTGAGTCAA 
CAGGATCAAA CACGGATTAT TAAGGAATTC GGATCTGCGC ATTTGAATCA AGATCCAACA 
AACTATCTTG ATTATACGTC TGGTACTTAT TCTAAAGCGC CGCCAGAGGT CCTTACTCAC 
GAAACGAACT CAAGTCACCT GGAATTCAAT CATGAATCAG AGTCACTGTT CAACTCTCCT 
AATACTTCTA GCTATTGTAA ACAAAAGTTT GTTCCAGGAA CATCTCCAGC AAAGCCTTCA 
AAGGTATGGA AGAGGCTAAA CACCATATTA GCAGATAATC TAAAGCTCAA GCGTGTATCA 
AAGTTTAATA GATCACTGTC TTTACCCGGT GATGTGCAAA GTCAAGGCCT CCAGAGACAG 
CCTCGTGGCC AGGCCGGTTC GTGTCCGTTT ATCCACAAGC GATCCAATCT GGCCGGTAGC 
CCGGTTCAGT TATCGAAAAG AATTCAAAAG CTTCCCATAC GTTTTATAGG ACGAGCGAAA 
GGAGTCCCAT TCGTGCGGCG CTCATCAAGC CCGGACTCAG CTGTTTCATT AGATTCCGCT 
GCCGATAAAC GCTTCTCAAG CGAAAAAGGT TTAAAAAAAA AAACAATTTC GTCCAAGATG 
AGTGGTTTAA TGCAAAAGGC AAAGACGTAC AAACGGCACA GCTTTGTCCT GCGCCGCGGC 
TGTAACATGT CGGATTCTGA ATTAGAGATG CCGGACTTCG TTTCATCGGG TAACGATAAT 
AGTTCAATAT CGACAAGAGA AATTTTGTTG AATCAATCGA TCGAGGTAGA AGATGAACAA 
GAAGATTTTA ATTATAAAAA TCGGTGTGAC TCTAAAAGTG TGTTAGGTGG TTCAATTGAG 
AAGCTAAATG GCAACCTAAC AAATAATCTA TTCCCCATTG TAGGTGACCT AAAAAAAATT 
CAAAGCCCCT TACCACTGGC TGTCTTAACA GAAATTCCTT CCTATAAGGA TGAATATAGC 
AATAAATCCG ATAGTATCAA AAATTCACCC ATTGAAATGC CAAAGATTCT CTTAGAAACG 
GCATGCAATC AAGAACTCAA TTTAGCACAT TCTGACGACG ATGTTGATAA GAACATACTG 
GCAAACTCGG CCAAAGACTA TGTAAATGCC CCCACATTTA GTATATTAAA GACAGTTGAA 
GATGCTTCCG AACCAACGAT GACACCACTC CATACAACAA CAACGACAAA CAGCAGTCTT 
AATGTCACAA GCGCTTTATG GGTCACGCAG CAGTGCTTAG ACCTTCCAAA CTATCCCGGG 
TGGGGGTCGC GAGAAGATGA CGATAACCGC AGTCAGCATT CTGCTCGCAC TCTATCTTCT 
TCTCGTCGAC AAAGCACTGA GGATAGCATT GATACAGACG ACGAATACTT TTATTATGAG 
CTTCGCCAGT TAGAAGAGCA AGAAAAGCAG AGGGCGCACA ATTCTGCAAT TCCATCTTGT 
GAACGACAAA ATGATAATGA TGTGTTGTTT TCACAAATTG GACAACTTCT TCAAAACGAT 
GTTAACGGAG GAGACGGTTT TCGCCATTCT AATGGGTGTA ATGATGGGGA AGACGCTATT 
ATTTTTTCTC CAAGTGAAAG TGTTAAGTTA CGAATGTCTG AAGTGTTTAA GGAACTGAAA 
AGCGTGGTTA GTTTAAACCC AAGCGTTAAT AATGACGCGA CTTTTGAGGG CGTTCCTATT 
GTAAAACCAA CATATGAAAA ACTTGAGACA GTCTCCGACT TGCATAGCGC TTGGCAGGAT 
GTTAATGGTG ATTTACAAAT TGCAGCTTCA GACATCGATT CTAATGAAGA TCTAGTTGGA 
AATAAGGGTA GGGAAACTCC AACTTATAAC AAACAGCGAA AATTGAGACG GTTAAAGAAA 
AAAACTCGCG ATCGTAAAAT AAATATATCT AAGAATGCAA CCAGTTCCAG TTCCTCTTGC 
CATTCAGAAA ATGAATGTAA TACACCCCTT GGTCAATGTA CCCAGAAATC GGTGGCAGAA 
AAAGATACAA ATGATATATC AAATAAATCG GAGGCTTCTA GCGAGACATC AGGACCAGAT 
ACGCCGGCAG AGCTTAGCGA TGTTGATATA AGCGAAACTG AAAACGGTTT GAGGGCTGAC 
GACGGCCAGA ATATAGATAA CATGCGTGGA AATTCAGGGT CTCTTTTAAA GGTGAATCGG 
AAGTACCTAT TACAATTTGA TGTTGACCAT AGTTTAATAA ACCAGCCCCT GGAAACATCT 
CAGTATAATA CGCACATGTT GGAGAATATT ACAAGTGCGT CAATTCCATC GCAGAATCGC 
CAAATAGACT CAAAAACGTT AATGAGTCAA AGCTCACATG CGGATGGATC CCAGGCTGTA 
GGCAATGAAA CCGCTGTAGG TCTAAGCAGT AGCAAGTGGA AATTGTTAAA AACTTTAAAA 
GAGCGCAAGA TCGAGGAAAA AAATAATCAA GATAAAATAA AAGAAGATGA AATGATAAAG 
GACAGGGACA AGAATGGAGG TGGTCCTGGA GAAGTGGGAT TACGTACAAA TGGTCATCCC 
GGTGACAATC CATTCTATAG CAATATTGAC AGTATGCCGG ACATAAGGCC TCGAAGAAAA 
TCCATTCCTC TTGTGTCTGA GCTGACAATG GCCGCTACAA AACGAAATGC TGGACTCACA 
TCTGCTGTTC CACGTGCAAC GCTGAATGAC GAAGATCTGA AAATGCACGT GTACAAAAAA 
GCTCTACAAG CCCTGATATA TCCTATATCC TCAACAACGC CCCACAATTT TTTGTTATGG 
ACTGCTACGT CACCAACGTA CTGCTACGAG TGTGAGGGTC TTTTGTGGGG GATAGCACGA 
CAAGGGGTCC GTTGCACTGA GTGCGGTGTA AAGTGTCATG AAAAATGTAA GGACCTTCTG 
AATGCGGACT GCCTGCAGCG TGCTGCCGAA AAAAGCTCAA AGCACGGTGC GGAAGACAAG 
GCTAACTCGA TTATTACAGC GATGAAAGAT CGTATGAAGC AACGTGAACG AGAGAAGCCT 
GAAATTTTTG AGTTGATTAG AGCCGTGTTC AGCGTTGAGG AGAAGAGTCA TGCCGGTCAT 
ATGAAGGCTG TGAAGCAAAG CGTCCTGGAT GGTACAAGCA AGTGGTCGGC CAAAATAGCA 
ATCACAGTGA TTTGTGCTCA AGGCCTGATA GCGAAGGATA AAAGCGGTAC CAGTGATCCA 
TATGTGACGG TGCAAGTTAG CAAGGTGAAA AAACGAACGC GAACAATGCC GCAGGAACTT 
AATCCTGTGT GGAACGAGAA GTTTCACTTC GAGTGCCACA ACTCCTCCGA CCGCATTAAG 
GTTCGTGTTT GGGACGAAGA CAATGACCTA AAGTCGAAAC TGCGCCAGAA GTTAACTCGA 
GAGTCGGACG ACTTTTTGGG ACAAACAATT ATCGAGGTTC GTACTCTTTC CGGTGAAATG 
GATGTATGGT ACAACCTGGA AAAGCGCACA GATAAATCGG CCGTATCAGG CGCGATACGG 
CTTCACATAT CTGTTGAAAT AAAGGGCGAG GAAAAAGTAG CTCCTTACCA CGTACAATAT 
ACCTGTTTAC ACGAGAACCT CTTCCATTAT CTTTGCGAAG AAAATACCGG AATGGTCAAG 
TTGCCAACTC AAAAAGGGGA CGATGCTTGG AAGTTGTATT TTGATGAAAT ACCCGAAGAG 
ATTGTCGACG AGTTTTCGAT GCGTTATGGA ATTGAGAATA TTTATCAAGC TATGACACAT 
TTCCACTGCT TGTCTGCTAA ATACCTATGT CCTGGGGTAC CAGCTGTAAT GAGTACCCTT 
TTGGCAAACA TAAATGCTTA TTATGCTCAT ACGACTGCTT CGTCAGCTGT ATCGGCCAGT 
GATAGATTTG CTGCCAGCAA TTTTGGGAAA GAAAAATTTG TTAAGTTGCT GGACCAGTTG 
CATAACTCAC TGAGGATCGA TTTGTCAATG TACAGAAATA ACTTTCCTGC ATCGAGTCCA 
GAAAAGCTAA TGGATCTAAA ATCTACAGTT GATTTATTGA CCAGCATAAC ATTTTTCCGC 
ATGAAGGTAC AAGAGCTATC TAGCCCGCCG AGAGCAAGTA CAGTAGTCAA GGATTGTGTT 
AAAGCATGCC TTAGATCCAC TTATCAATTC TTATTTGAAA ATTGTTATGA ACTGTACAAC 
AGAGAATTTC AGGTTGATCC CAACGAAGCA AAACGTGCAC CAGATGATCA TGAACCCAAG 
CTTGATAGCG TCGACTTTTG GCATAAGCTA ATAGCTTTAA TCGTATCGGT AATAGATGAA 
GACAAAAACA GTTACGGAAC CGTTTTAAAT CAATTTCCGC AAGAGCTAAA TATTGGACAG 
TTATCAGCAT CTTCAATGTG GCATCTCTTT GCTGTGGACA TGAAGTACGC GCTCGAGGAG 
CATGAGCAAC ATCGTCTTTG CAAGTCATCG GCTTATATGA ATCTTCACTT TCGAGTTAAG 
TGGCTATATA GTAACTACGT CAAAGAGGTG CCTCCCTACA AAGGAGCGGT TCCCGATTAC 
CCAGCATGGT TCGAGCCTTT TGTGATGCAG TGGCTAAATG AAAATGACGA CGTGTCATTG 
GAATACCTTC ATGGCGCATT TAAGAGGGAT AAAAAAGACG GGTTTCAAAA GAGCAGCGAG 
CACGCGCTCT TTTCAAATTC TGTAGTCGAC GTTTTTACGC AATTGACGCA GTGTTTCGAT 
GTTGTTAGCA AACTCGAGTG TCCTGATCCA GAAATTTGGA AACGTTACAT GAGACGTTTT 
GCTAAAACAA TCGTAAAAGT GCTAATAGCC TATGCGGATA TTGTGAAACT TGAGTTTCCA 
GAGCATATGA AGGATGAAAG AATTGCTTGC ATACTTATGA ACAACATTCA GCAACTAAGG 
GTGCAGCTAG AAAAAATGTT TGAGTCTATG GGTGGGGATA AGCTAGAGGA GGATGCAGCC 
AATATTTTGA AAGAACTTCA GCAAAATCTT AACTCTGCGT TGGACGACTT AGCTTCACAA 
TTCGCTATCA GTTTGGAGCC ACGAATAACT CAATCAGTGC GAGAACTTGG AGACATGCTG 
CTTTCTATTA AAGGAGGCAG TGGAACCCTT GCTGCTGGTA ATCTTGCAGC TCAAAGGAAC 
GCGGTCGCCG TTGAGGCCGA TGAAGTTTTA AGGCCGCTTA TGGATTTACT TGATGGCTCT 
TTAACTCTCT ATGCGCAATC ATGTGAAAAA ACCGTGTTGA AGAGGTTACT AAAGGAGTTA 
TGGAAGATCG TAATGCGAAT ATTAGAGAAA ACAATTGTTT TACCACCTAT GACTGATAAA 
ACTATGATGT TTAAACACCT TACTGACAAT GCAAAAAACT TGGCATCGAA TGCAAAAATT 
GAAGACATGG GTCGTTTGTT CAAGTCACAT ATGGCAGGAA AGCAGGATGT TAAGAGTGCT 
CTGAGCGGGG TAATGGACAT ATCAAAAGAA GTAGAAAAAA ACTTGTCACC CAAGCAATGC 
GCAGTGCTTG ATGTTGCCTT AGATACAATA AAACAATACT TTCACGCGGG TGGCAATGGA 
CTGAAAAAGA CCTTTCTCGA AAAGTCGCCC GAGCTTCAGA GTTTACGTTA CGCACTCAGT 
TTATATACAC AAATGACCGA CACACTAATC AAAACCTTTA TATCTAGCCA AGTTCACGAG 
GTCGATCTAG AAAACTCAGA GGAAAGTGTT GGGGAAATAT CCGTGCAAAT AGACTTATTT 
TCCCATCCCG GCACCGGTGA ACATAAAGTA AACGTGAAAG TTGTTGCTGC TAATGACCTT 
AAATGGCAAA TACCATCGGG TATGTTCAGA CCATTTGTTG ACATTAATTT AATAGGACCG 
CACCTGCAAG AAAAAAAAAG GAAGTTCGCT ACAAAGTCGA AGTCTAACAA CTGGTCGCCT 
AAATACAACG AATCTTTCAG CTTCACCATT GGAAATGAGG AGCAGCTGGA CTTCTTTGAA 
TTGCACATTT GCGTTAAGGA CTATTGTTTT GCTCGTGATG ACCGACTTGT GGGCGTGGCT 
GTAATTCCAT TAAAAGATAT ATCGGAAAAG GGATCGGTCG CATGCTGGTT ACCCTTAATG 
AGACGAATTG AAATGGACGA AACGGGCTGG ACCATTTTAA GAATTTTGTC ACAGCGAAAT 
AATGATGAAG TGGCCAAGGA ATTTGTCAAG CTTAAATCGG AAATACGGCA GGAGCCAACC 
ATGGGTACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_726615.1
CDS	162..9713
Translation	MMNTSQLQVTGDTEKKSQLLKKELKINTQEKLIFAENALKSQIKIKEQLRLQQQSTIYASSLLSSSAAGSVRAPLLSQGHLNSIQHNMDFDLAKAQIPEMQPPMSKSPNGLDFSYLSYPSINTNESMISIKSEQQLCQSYNSEQHSDYIISDYMDKIATRISLLETELKFAWRALDLLSTEYGKIWIRLEKLENISIEQQSVVGNLVDLIGASKKELQKVDIERMKVPLYQDEDQLLPLEMEDTLDIDIQSSNRDFDKNLTFENHEKTFVTKHTQATKSEDLMNSAYAIDSHPNFENIDFNGKNLDIGIIKFGFEKGYEPKQKGNQSDFEAYKESRTKLYSDSDLMLYERQQFLANSARAELMKEFLNGRRVLNEISASSGALSKSSEKDKNLVKPREPLLSEIDDTYVRRSTCELVSPSSNNFFQIAASKTGEGGNQVLPIEDTGGTESPANINEVDESFYKNLNEAYRDNELSSEIFKVDALLHQSEATHDHISFIRNQSTSSPVLNNQRVNAITGTQPLQTAKACLVTEPNQHLNKEPRKHRKKKHHTNEMDMINKLKCILAQAQSTEIIKRDIEELGSKLSDACVKQTETENKSDNHISTSYLKDDIRKMLNNVIQTLLGEINKIKGLQSLSATQLSQLQNAVWSEERFFQKISLVDKKLTLLLLNPLTVTEELQRLCISNTNEKFVLVIKKLKKNIDTLKKLVGSSLDDFKIDNSHANMTTFHSLNPSHNSNDSSFSAHLLRNNSNLDEQLKILETQEIEIHRKKKIDEIVSGLSEETYTFNPDMEYKSQHSCTNSTDNIIGFSKNIYNEDEYIKSLRKSLERHNSMIFLLHLQNPEKHKVLADINDAQMDSNRASLSPPPPAPTDTVYLNDAINQITYQQRNGKSDSGLSSMSGWSANSQVSVGLQNYDPACNNILENCSERLQTYHSFPLAENSNFHSFPLSHEQAQTTNQTEFVILEENLNYIYELSKNLPICSAYENKSIFDMKYEICDSNEIGKFSTVDEMLEWDQLNEPDKQNFSGKRLNSNLMPDLLTAQIPNSISKHNKNTSVNIEYVRQKENKGMDRRSIIEPNIYNGKSEDQICRPCLTDKLVFYPSSNSITDHNSSHDFNCLSQQDQTRIIKEFGSAHLNQDPTNYLDYTSGTYSKAPPEVLTHETNSSHLEFNHESESLFNSPNTSSYCKQKFVPGTSPAKPSKVWKRLNTILADNLKLKRVSKFNRSLSLPGDVQSQGLQRQPRGQAGSCPFIHKRSNLAGSPVQLSKRIQKLPIRFIGRAKGVPFVRRSSSPDSAVSLDSAADKRFSSEKGLKKKTISSKMSGLMQKAKTYKRHSFVLRRGCNMSDSELEMPDFVSSGNDNSSISTREILLNQSIEVEDEQEDFNYKNRCDSKSVLGGSIEKLNGNLTNNLFPIVGDLKKIQSPLPLAVLTEIPSYKDEYSNKSDSIKNSPIEMPKILLETACNQELNLAHSDDDVDKNILANSAKDYVNAPTFSILKTVEDASEPTMTPLHTTTTTNSSLNVTSALWVTQQCLDLPNYPGWGSREDDDNRSQHSARTLSSSRRQSTEDSIDTDDEYFYYELRQLEEQEKQRAHNSAIPSCERQNDNDVLFSQIGQLLQNDVNGGDGFRHSNGCNDGEDAIIFSPSESVKLRMSEVFKELKSVVSLNPSVNNDATFEGVPIVKPTYEKLETVSDLHSAWQDVNGDLQIAASDIDSNEDLVGNKGRETPTYNKQRKLRRLKKKTRDRKINISKNATSSSSSCHSENECNTPLGQCTQKSVAEKDTNDISNKSEASSETSGPDTPAELSDVDISETENGLRADDGQNIDNMRGNSGSLLKVNRKYLLQFDVDHSLINQPLETSQYNTHMLENITSASIPSQNRQIDSKTLMSQSSHADGSQAVGNETAVGLSSSKWKLLKTLKERKIEEKNNQDKIKEDEMIKDRDKNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_166794.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant B (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_166794.3

ORF Insert Sequence:

ATGATGAACA CATCTCAGCT GCAAGTGACC GGGGATACGG AAAAAAAAAG TCAGTTGCTT 
AAAAAGGAAC TCAAAATAAA CACCCAGGAA AAGCTTATCT TTGCAGAAAA TGCCCTCAAA 
AGCCAGATTA AAATAAAGGA GCAGTTACGA CTTCAGCAAC AATCAACGAT TTATGCGTCG 
TCACTATTGA GCTCTTCTGC TGCCGGATCA GTAAGAGCTC CGCTCTTAAG CCAGGGGCAC 
CTTAATTCTA TCCAACACAA CATGGATTTT GACCTTGCCA AAGCACAAAT ACCGGAAATG 
CAACCACCGA TGAGTAAGTC GCCCAATGGA TTGGATTTCT CTTACTTAAG TTATCCAAGC 
ATAAATACTA ATGAAAGTAT GATATCGATT AAAAGTGAAC AACAGTTGTG TCAGAGCTAC 
AATTCGGAGC AACACTCTGA CTACATTATC TCCGATTATA TGGATAAAAT TGCAACAAGG 
ATCAGTTTAC TCGAGACAGA GCTGAAGTTT GCTTGGCGTG CATTGGATTT GCTTTCTACG 
GAGTACGGAA AGATTTGGAT TCGTTTGGAA AAGCTTGAAA ACATATCAAT AGAACAACAG 
TCTGTCGTTG GCAACCTTGT GGATCTTATC GGCGCATCAA AAAAAGAACT TCAAAAAGTG 
GATATCGAAA GAATGAAAGT ACCCTTATAT CAAGATGAAG ATCAATTGCT ACCGTTGGAA 
ATGGAAGACA CATTAGATAT AGATATCCAA TCATCTAATC GTGACTTTGA TAAGAATCTT 
ACTTTTGAAA ATCATGAAAA AACTTTTGTA ACAAAACATA CGCAAGCTAC TAAATCGGAA 
GACCTTATGA ATTCCGCTTA CGCAATAGAT TCCCATCCAA ATTTTGAAAA CATTGATTTC 
AATGGCAAAA ACTTAGACAT TGGGATAATA AAATTTGGTT TTGAAAAAGG CTACGAGCCA 
AAACAAAAAG GTAATCAATC GGATTTTGAA GCATACAAGG AATCAAGAAC TAAACTTTAC 
TCAGATTCTG ACCTTATGTT ATATGAGAGG CAGCAGTTTC TAGCAAATAG CGCGCGTGCC 
GAATTAATGA AAGAATTTTT AAATGGACGT CGAGTTTTAA ATGAGATTTC GGCATCCTCG 
GGTGCATTGT CAAAAAGTTC TGAGAAGGAT AAAAATCTTG TAAAGCCTAG AGAACCACTG 
TTAAGTGAGA TAGATGATAC ATACGTGAGA CGGTCAACCT GTGAACTTGT CAGCCCTAGT 
AGTAATAATT TTTTTCAAAT AGCAGCCTCT AAAACAGGCG AGGGTGGAAA TCAGGTTTTA 
CCCATTGAAG ACACTGGAGG AACAGAAAGT CCAGCTAATA TTAATGAAGT AGACGAATCT 
TTTTATAAAA ATCTAAACGA GGCTTATCGA GACAATGAAT TGAGCTCTGA GATATTTAAG 
GTTGATGCAT TGCTACATCA GTCTGAGGCC ACACATGACC ACATCTCATT TATCCGAAAC 
CAGTCAACTT CTTCCCCAGT TTTAAATAAT CAGAGGGTGA ATGCTATTAC AGGTACACAA 
CCTTTACAGA CTGCAAAGGC GTGTCTCGTA ACAGAGCCAA ACCAACATTT GAACAAAGAA 
CCAAGAAAGC ACAGAAAAAA AAAACATCAT ACAAACGAAA TGGATATGAT TAATAAACTA 
AAGTGTATCC TTGCACAAGC ACAATCAACA GAGATTATTA AGAGAGACAT TGAAGAATTG 
GGTTCGAAAC TTTCAGATGC ATGTGTAAAA CAAACTGAAA CGGAAAATAA ATCAGACAAC 
CATATTAGTA CCAGTTATTT AAAAGATGAT ATACGAAAAA TGTTAAACAA CGTAATACAG 
ACTTTATTGG GGGAAATAAA TAAAATTAAA GGGTTACAAT CATTGAGTGC AACGCAATTG 
AGTCAGTTAC AAAATGCCGT ATGGTCGGAA GAAAGGTTTT TTCAAAAAAT TAGTCTGGTC 
GATAAAAAGT TGACTTTGCT GTTACTTAAT CCGTTAACTG TTACTGAAGA ACTGCAAAGG 
CTTTGCATTT CAAACACAAA TGAAAAGTTT GTTCTGGTAA TAAAAAAGTT AAAAAAAAAT 
ATTGACACTC TAAAAAAATT AGTTGGCAGC TCATTGGATG ACTTTAAAAT AGATAATTCA 
CACGCAAATA TGACCACATT TCACAGCCTT AATCCATCCC ATAATTCTAA TGATAGCAGT 
TTTAGTGCAC ATTTATTAAG AAATAACTCC AATCTGGATG AACAGTTGAA AATTCTTGAA 
ACTCAAGAGA TTGAAATACA CCGGAAAAAG AAAATAGACG AAATTGTGAG CGGACTTTCC 
GAAGAAACCT ATACTTTTAA CCCAGATATG GAATATAAAA GCCAACATAG TTGTACCAAC 
TCAACTGATA ATATTATAGG ATTCTCAAAA AATATTTACA ATGAAGATGA ATATATAAAG 
TCTTTAAGAA AGAGTCTAGA ACGCCACAAT AGCATGATCT TTTTACTACA TTTGCAAAAT 
CCTGAAAAAC ACAAAGTGTT GGCTGATATA AATGATGCAC AAATGGATTC AAATCGAGCT 
AGTCTCAGTC CTCCACCACC AGCTCCAACC GACACAGTTT ACTTAAATGA TGCAATTAAT 
CAAATTACAT ATCAACAACG AAATGGAAAA TCAGACTCTG GCCTATCGTC GATGAGCGGC 
TGGTCAGCCA ACTCCCAAGT GTCGGTTGGA CTTCAAAATT ATGATCCTGC ATGTAATAAT 
ATTTTGGAAA ATTGTAGCGA AAGATTGCAA ACATACCATT CATTTCCTTT GGCTGAAAAT 
TCGAACTTTC ACAGTTTCCC ACTCAGTCAT GAACAAGCTC AAACTACTAA TCAAACTGAG 
TTTGTAATAT TAGAAGAAAA TCTAAACTAT ATTTATGAGC TTTCTAAAAA TTTACCTATT 
TGCTCTGCCT ATGAAAATAA ATCTATTTTC GATATGAAAT ATGAAATTTG CGACTCAAAT 
GAAATTGGAA AATTTTCCAC TGTGGACGAA ATGCTGGAAT GGGACCAGCT AAATGAACCG 
GATAAACAAA ACTTTTCTGG GAAACGGTTA AACTCCAACC TTATGCCAGA TCTCTTGACT 
GCTCAAATAC CAAATTCCAT ATCAAAACAC AATAAAAACA CAAGTGTTAA CATTGAATAT 
GTTAGACAAA AAGAAAATAA AGGCATGGAT AGGCGAAGCA TCATAGAACC AAATATTTAT 
AACGGAAAAT CGGAAGATCA AATTTGCAGA CCTTGTTTGA CAGATAAACT CGTATTTTAT 
CCCTCATCGA ACTCTATTAC CGATCACAAT AGCAGCCATG ATTTCAATTG TTTGAGTCAA 
CAGGATCAAA CACGGATTAT TAAGGAATTC GGATCTGCGC ATTTGAATCA AGATCCAACA 
AACTATCTTG ATTATACGTC TGGTACTTAT TCTAAAGCGC CGCCAGAGGT CCTTACTCAC 
GAAACGAACT CAAGTCACCT GGAATTCAAT CATGAATCAG AGTCACTGTT CAACTCTCCT 
AATACTTCTA GCTATTGTAA ACAAAAGTTT GTTCCAGGAA CATCTCCAGC AAAGCCTTCA 
AAGGTATGGA AGAGGCTAAA CACCATATTA GCAGATAATC TAAAGCTCAA GCGTGTATCA 
AAGTTTAATA GATCACTGTC TTTACCCGGT GATGTGCAAA GTCAAGGCCT CCAGAGACAG 
CCTCGTGGCC AGGCCGGTTC GTGTCCGTTT ATCCACAAGC GATCCAATCT GGCCGGTAGC 
CCGGTTCAGT TATCGAAAAG AATTCAAAAG CTTCCCATAC GTTTTATAGG ACGAGCGAAA 
GGAGTCCCAT TCGTGCGGCG CTCATCAAGC CCGGACTCAG CTGTTTCATT AGATTCCGCT 
GCCGATAAAC GCTTCTCAAG CGAAAAAGGT TTAAAAAAAA AAACAATTTC GTCCAAGATG 
AGTGGTTTAA TGCAAAAGGC AAAGACGTAC AAACGGCACA GCTTTGTCCT GCGCCGCGGC 
TGTAACATGT CGGATTCTGA ATTAGAGATG CCGGACTTCG TTTCATCGGG TAACGATAAT 
AGTTCAATAT CGACAAGAGA AATTTTGTTG AATCAATCGA TCGAGGTAGA AGATGAACAA 
GAAGATTTTA ATTATAAAAA TCGGTGTGAC TCTAAAAGTG TGTTAGGTGG TTCAATTGAG 
AAGCTAAATG GCAACCTAAC AAATAATCTA TTCCCCATTG TAGGTGACCT AAAAAAAATT 
CAAAGCCCCT TACCACTGGC TGTCTTAACA GAAATTCCTT CCTATAAGGA TGAATATAGC 
AATAAATCCG ATAGTATCAA AAATTCACCC ATTGAAATGC CAAAGATTCT CTTAGAAACG 
GCATGCAATC AAGAACTCAA TTTAGCACAT TCTGACGACG ATGTTGATAA GAACATACTG 
GCAAACTCGG CCAAAGACTA TGTAAATGCC CCCACATTTA GTATATTAAA GACAGTTGAA 
GATGCTTCCG AACCAACGAT GACACCACTC CATACAACAA CAACGACAAA CAGCAGTCTT 
AATGTCACAA GCGCTTTATG GGTCACGCAG CAGTGCTTAG ACCTTCCAAA CTATCCCGGG 
TGGGGGTCGC GAGAAGATGA CGATAACCGC AGTCAGCATT CTGCTCGCAC TCTATCTTCT 
TCTCGTCGAC AAAGCACTGA GGATAGCATT GATACAGACG ACGAATACTT TTATTATGAG 
CTTCGCCAGT TAGAAGAGCA AGAAAAGCAG AGGGCGCACA ATTCTGCAAT TCCATCTTGT 
GAACGACAAA ATGATAATGA TGTGTTGTTT TCACAAATTG GACAACTTCT TCAAAACGAT 
GTTAACGGAG GAGACGGTTT TCGCCATTCT AATGGGTGTA ATGATGGGGA AGACGCTATT 
ATTTTTTCTC CAAGTGAAAG TGTTAAGTTA CGAATGTCTG AAGTGTTTAA GGAACTGAAA 
AGCGTGGTTA GTTTAAACCC AAGCGTTAAT AATGACGCGA CTTTTGAGGG CGTTCCTATT 
GTAAAACCAA CATATGAAAA ACTTGAGACA GTCTCCGACT TGCATAGCGC TTGGCAGGAT 
GTTAATGGTG ATTTACAAAT TGCAGCTTCA GACATCGATT CTAATGAAGA TCTAGTTGGA 
AATAAGGGTA GGGAAACTCC AACTTATAAC AAACAGCGAA AATTGAGACG GTTAAAGAAA 
AAAACTCGCG ATCGTAAAAT AAATATATCT AAGAATGCAA CCAGTTCCAG TTCCTCTTGC 
CATTCAGAAA ATGAATGTAA TACACCCCTT GGTCAATGTA CCCAGAAATC GGTGGCAGAA 
AAAGATACAA ATGATATATC AAATAAATCG GAGGCTTCTA GCGAGACATC AGGACCAGAT 
ACGCCGGCAG AGCTTAGCGA TGTTGATATA AGCGAAACTG AAAACGGTTT GAGGGCTGAC 
GACGGCCAGA ATATAGATAA CATGCGTGGA AATTCAGGGT CTCTTTTAAA GGTGAATCGG 
AAGTACCTAT TACAATTTGA TGTTGACCAT AGTTTAATAA ACCAGCCCCT GGAAACATCT 
CAGTATAATA CGCACATGTT GGAGAATATT ACAAGTGCGT CAATTCCATC GCAGAATCGC 
CAAATAGACT CAAAAACGTT AATGAGTCAA AGCTCACATG CGGATGGATC CCAGGCTGTA 
GGCAATGAAA CCGCTGTAGG TCTAAGCAGT AGCAAGTGGA AATTGTTAAA AACTTTAAAA 
GAGCGCAAGA TCGAGGAAAA AAATAATCAA GATAAAATAA AAGAAGATGA AATGATAAAG 
GACAGGGACA AGAATGGAGG TGGTCCTGGA GAAGTGGGAT TACGTACAAA TGGTCATCCC 
GGTGACAATC CATTCTATAG CAATATTGAC AGTATGCCGG ACATAAGGCC TCGAAGAAAA 
TCCATTCCTC TTGTGTCTGA GCTGACAATG GCCGCTACAA AACGAAATGC TGGACTCACA 
TCTGCTGTTC CACGTGCAAC GCTGAATGAC GAAGATCTGA AAATGCACGT GTACAAAAAA 
GCTCTACAAG CCCTGATATA TCCTATATCC TCAACAACGC CCCACAATTT TTTGTTATGG 
ACTGCTACGT CACCAACGTA CTGCTACGAG TGTGAGGGTC TTTTGTGGGG GATAGCACGA 
CAAGGGGTCC GTTGCACTGA GTGCGGTGTA AAGTGTCATG AAAAATGTAA GGACCTTCTG 
AATGCGGACT GCCTGCAGCG TGCTGCCGAA AAAAGCTCAA AGCACGGTGC GGAAGACAAG 
GCTAACTCGA TTATTACAGC GATGAAAGAT CGTATGAAGC AACGTGAACG AGAGAAGCCT 
GAAATTTTTG AGTTGATTAG AGCCGTGTTC AGCGTTGAGG AGAAGAGTCA TGCCGGTCAT 
ATGAAGGCTG TGAAGCAAAG CGTCCTGGAT GGTACAAGCA AGTGGTCGGC CAAAATAGCA 
ATCACAGTGA TTTGTGCTCA AGGCCTGATA GCGAAGGATA AAAGCGGTAC CAGTGATCCA 
TATGTGACGG TGCAAGTTAG CAAGGTGAAA AAACGAACGC GAACAATGCC GCAGGAACTT 
AATCCTGTGT GGAACGAGAA GTTTCACTTC GAGTGCCACA ACTCCTCCGA CCGCATTAAG 
GTTCGTGTTT GGGACGAAGA CAATGACCTA AAGTCGAAAC TGCGCCAGAA GTTAACTCGA 
GAGTCGGACG ACTTTTTGGG ACAAACAATT ATCGAGGTTC GTACTCTTTC CGGTGAAATG 
GATGTATGGT ACAACCTGGA AAAGCGCACA GATAAATCGG CCGTATCAGG CGCGATACGG 
CTTCACATAT CTGTTGAAAT AAAGGGCGAG GAAAAAGTAG CTCCTTACCA CGTACAATAT 
ACCTGTTTAC ACGAGAACCT CTTCCATTAT CTTTGCGAAG AAAATACCGG AATGGTCAAG 
TTGCCAACTC AAAAAGGGGA CGATGCTTGG AAGTTGTATT TTGATGAAAT ACCCGAAGAG 
ATTGTCGACG AGTTTTCGAT GCGTTATGGA ATTGAGAATA TTTATCAAGC TATGACACAT 
TTCCACTGCT TGTCTGCTAA ATACCTATGT CCTGGGGTAC CAGCTGTAAT GAGTACCCTT 
TTGGCAAACA TAAATGCTTA TTATGCTCAT ACGACTGCTT CGTCAGCTGT ATCGGCCAGT 
GATAGATTTG CTGCCAGCAA TTTTGGGAAA GAAAAATTTG TTAAGTTGCT GGACCAGTTG 
CATAACTCAC TGAGGATCGA TTTGTCAATG TACAGAAATA ACTTTCCTGC ATCGAGTCCA 
GAAAAGCTAA TGGATCTAAA ATCTACAGTT GATTTATTGA CCAGCATAAC ATTTTTCCGC 
ATGAAGGTAC AAGAGCTATC TAGCCCGCCG AGAGCAAGTA CAGTAGTCAA GGATTGTGTT 
AAAGCATGCC TTAGATCCAC TTATCAATTC TTATTTGAAA ATTGTTATGA ACTGTACAAC 
AGAGAATTTC AGGTTGATCC CAACGAAGCA AAACGTGCAC CAGATGATCA TGAACCCAAG 
CTTGATAGCG TCGACTTTTG GCATAAGCTA ATAGCTTTAA TCGTATCGGT AATAGATGAA 
GACAAAAACA GTTACGGAAC CGTTTTAAAT CAATTTCCGC AAGAGCTAAA TATTGGACAG 
TTATCAGCAT CTTCAATGTG GCATCTCTTT GCTGTGGACA TGAAGTACGC GCTCGAGGAG 
CATGAGCAAC ATCGTCTTTG CAAGTCATCG GCTTATATGA ATCTTCACTT TCGAGTTAAG 
TGGCTATATA GTAACTACGT CAAAGAGGTG CCTCCCTACA AAGGAGCGGT TCCCGATTAC 
CCAGCATGGT TCGAGCCTTT TGTGATGCAG TGGCTAAATG AAAATGACGA CGTGTCATTG 
GAATACCTTC ATGGCGCATT TAAGAGGGAT AAAAAAGACG GGTTTCAAAA GAGCAGCGAG 
CACGCGCTCT TTTCAAATTC TGTAGTCGAC GTTTTTACGC AATTGACGCA GTGTTTCGAT 
GTTGTTAGCA AACTCGAGTG TCCTGATCCA GAAATTTGGA AACGTTACAT GAGACGTTTT 
GCTAAAACAA TCGTAAAAGT GCTAATAGCC TATGCGGATA TTGTGAAACT TGAGTTTCCA 
GAGCATATGA AGGATGAAAG AATTGCTTGC ATACTTATGA ACAACATTCA GCAACTAAGG 
GTGCAGCTAG AAAAAATGTT TGAGTCTATG GGTGGGGATA AGCTAGAGGA GGATGCAGCC 
AATATTTTGA AAGAACTTCA GCAAAATCTT AACTCTGCGT TGGACGACTT AGCTTCACAA 
TTCGCTATCA GTTTGGAGCC ACGAATAACT CAATCAGTGC GAGAACTTGG AGACATGCTG 
CTTTCTATTA AAGGAGGCAG TGGAACCCTT GCTGCTGGTA ATCTTGCAGC TCAAAGGAAC 
GCGGTCGCCG TTGAGGCCGA TGAAGTTTTA AGGCCGCTTA TGGATTTACT TGATGGCTCT 
TTAACTCTCT ATGCGCAATC ATGTGAAAAA ACCGTGTTGA AGAGGTTACT AAAGGAGTTA 
TGGAAGATCG TAATGCGAAT ATTAGAGAAA ACAATTGTTT TACCACCTAT GACTGATAAA 
ACTATGATGT TTAAACACCT TACTGACAAT GCAAAAAACT TGGCATCGAA TGCAAAAATT 
GAAGACATGG GTCGTTTGTT CAAGTCACAT ATGGCAGGAA AGCAGGATGT TAAGAGTGCT 
CTGAGCGGGG TAATGGACAT ATCAAAAGAA GTAGAAAAAA ACTTGTCACC CAAGCAATGC 
GCAGTGCTTG ATGTTGCCTT AGATACAATA AAACAATACT TTCACGCGGG TGGCAATGGA 
CTGAAAAAGA CCTTTCTCGA AAAGTCGCCC GAGCTTCAGA GTTTACGTTA CGCACTCAGT 
TTATATACAC AAATGACCGA CACACTAATC AAAACCTTTA TATCTAGCCA AGTTCACGAG 
GTCGATCTAG AAAACTCAGA GGAAAGTGTT GGGGAAATAT CCGTGCAAAT AGACTTATTT 
TCCCATCCCG GCACCGGTGA ACATAAAGTA AACGTGAAAG TTGTTGCTGC TAATGACCTT 
AAATGGCAAA TACCATCGGG TATGTTCAGA CCATTTGTTG ACATTAATTT AATAGGACCG 
CACCTGCAAG AAAAAAAAAG GAAGTTCGCT ACAAAGTCGA AGTCTAACAA CTGGTCGCCT 
AAATACAACG AATCTTTCAG CTTCACCATT GGAAATGAGG AGCAGCTGGA CTTCTTTGAA 
TTGCACATTT GCGTTAAGGA CTATTGTTTT GCTCGTGATG ACCGACTTGT GGGCGTGGCT 
GTAATTCCAT TAAAAGATAT ATCGGAAAAG GGATCGGTCG CATGCTGGTT ACCCTTAATG 
AGACGAATTG AAATGGACGA AACGGGCTGG ACCATTTTAA GAATTTTGTC ACAGCGAAAT 
AATGATGAAG TGGCCAAGGA ATTTGTCAAG CTTAAATCGG AAATACGGCA GGAGCCAACC 
ATGGGTACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-13 ( NM_001272146.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001259075.1 Drosophila melanogaster unc-13, transcript variant F (unc-13), mRNA.

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CloneID	OFa00317
Clone ID Related Accession (Same CDS sequence)	NM_001272146.1
Accession Version	NM_001272146.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGACGCGGC TCAGAGGGAT 
GGACGCGGCT TCGACACAGA TCAAACTGAT GCGATGGGTT CTGAATCTAG TACATATGAG 
GTTTATGAAA AAATGCAAAG GCCATACACG TCCATGTTGC CGTTAGATTA CAGTGATTAC 
CAAGAAGGTT GTTACAATAC CGACAATCTT TCAACATACA GCGACACACC GCCGAGCAAT 
AATACACAAC TAAAGAGGCA AATGCAGAGG AAAATTTCGT TAATGATGGC GATGACTACA 
GCTTCTGTGA TAGCATCTGG AAACACCATC AGCACCAATG CCGCTGCAAG AGACTTAGAC 
AGATGTTTGG CGACGGAATC TTGTGAAGTA ATTGTCGATA CACGTGACTC TGGTAGCGTC 
ACAAGTTTCC CGTCATCTGC TGTGACTGCA ATAACAAAGA CTAGAAAATT GCCAAAGGTT 
TTACCAACGC CACTGTGTAA ATCATCTCGT CATCCAATCA CCATAGCCAC CGATGCATTA 
AGTTCATCAT ATACTTCAGA TCCTTTACCA GAGAAGAGTC ATCGCCCAAA GCAACTGCCA 
AAGCTGCCTA TATCATTACC CCAGTCCAAT GATCGTGCAA GTCTCAATTC GAATTGGGCG 
ACCCCTCCTG CCCCAGATGC ATTACCATTT AATTCGTTCG ACCATAAATC GGCATCTTCT 
CCTACGCCAA CAACAACAAT AACAAAAGAT ACGGAAACCA CCTCATATCT GGTTGAAACA 
GATTTTATTG GAGCCAGGCA TAATGCCCTT TATCAGTATG ATAGTAAAGA ACCAAATATA 
GTATTTTCGG ACAAGTCTGT CGAAGCAGAA CACTCGCCAA CGTGGACCCC ATTATCGCCA 
ATACAATCCA AACAAAGTCC TTGTCCGCCT GTGGCGCTAC CAAGCAATAT TATGCAAAAT 
GTTTCTCTAA CATGTCACTT ACCTGAAATC GAAGCAACCC GATCCGATAT TGAGCGAGAA 
CCAGAAAGCT CTTCTATCGA ACCAATTCTA GAAATTGAAA AATTAGCCGA TCCATATAGC 
GGGCCCGGAT CAGCCTTATT TAACATATCT GAGTATCTGA AGCCTTATAC ATTAAATAAA 
CCTATTTTGT CCGAAGAAAA GAAAAACCAT ATCGCCAATG CTGCCAGTAC GTCAACCACA 
ACGCCATTGA ATATAACATC AGATGACGAA TTCTCTTCAT ATTCTAACAA GTGGACGAGT 
ACTTGCGTTA ATTTTCAACC ATTAGACGTT GAATCAAGCT TAAATATTTC ACTTAAAGTC 
AACGCTGGCA CGAATCAGGC CGAATTATTA ATGACACCAT TAAAAAGTTC CACTCCTCTT 
TTCATTTCTT CTAATGGCAC ATCCGATAAT TTTAATTTGA GGAAAAGCTC TCCTCCCGAC 
AGCGCGTTCA CCACCACGGT TAACGTCAAC AGTTTTGAAA CGGTACTAGT ATCTGGAAGC 
CAAACTGCCT CACCGTCGCC GTCAAACTTA AAGTCACCAC CATCAATAGC TCCATTATTA 
TCCTACTCGG ATTATATGAA ACAATTTGAG TTACCAGAAT TGCCGCAACC CATTATGGAT 
CTTTCAGAAA ATGATACTGC TACTCAAAGC GATTCCTTTA ATGTAATCAA TAATACTCTT 
ACGAACGCTG ACAATTTAAA TTCCTACAAC CAGATGGATG TAGAAAGCAA AAGTAGTCTA 
CAGTTACCTT CTTATTCCAG TGAATCATTT GATCCGTGTA GTGTGCCTAG TTTTTCAATT 
AAGAACAAAG AATATAAAAT TGTCGAAAAG CTGGACAGTC TATCAAATGT TGAAAGCGTA 
GAATCACCGA AAACCTTAGT AAGTCCAGTA AACCCATTGA ATTGCTCAAA ATTATTGCCA 
GGGACGGAAT CAATAGTGAG TAATGATGTA GCGTTCGATG ATACATTTTA CGATAGCTTT 
AATGTTGACA TAAAAGAACT GACCGCATTT GTTGACCATG TTGCCCCAGA GGACGGTTTA 
TACAATTTCC CAAACGATAA GACTTCAGTT GAATTCAGTT TTGATAAGAC CGAGGATACA 
ATAGACATGA ATCAGAACTT AAGCTCAGGA GAATGTGGTT ATTATAAGCC ATCTCAGGCA 
CAACAAAAAG CTAGTGTAGT AGCATCCGCA GCAAGTAGTG TTCTTGACGG AATTTCAAAG 
GGCTTAAAAG GGGGGCTGGA CGGCGTTTTT AGTGGTGTCA GTTCAACAGT TGATGTCACT 
CAATCAAATC CCAGTTCCAA AAGAGGTTTT AGCTTTAACT TAGCATCAAA GATTGTGCCA 
AGTGTCGGTG GATTGCTAAC TAGTACAAGT TCCACCTCTA TAAAACAAAC AGGATCAGAA 
ACTAATCCTA CCCTAATATT GATTTCACCT GAAAATGTTT CTAGTCGTAA CAGTAATTAT 
ATACCTACGA CATCTCCATC TTGTACTCAA AAAAATGGCG AGGAAAATTT ATATTCGGCT 
ACAGTCCACA ATAAGTCAAC GAAATCGAAT TCATACTATA ATGAAGTGGG AGAAATTAGT 
TCGACCTTAG TCAGAAATGT TTGTGACAGT TACGATAATA GCTATGATGA GATGATTTTA 
ACCAACGAAA TGGTAAATAT AGGGATGCTC GACAGTGAAT CTGAGTTTGG CTTAATAGAA 
AATTCATATT CGTATCAGGT ACCAGACAAT GAGCAAATCG ACAGTGTTAA CTCGTATAAT 
AATAAGACCC AAAATGTAAC AAATAATGGC ATAGAAAAGG CAAATACAAA AAATAAACCA 
GTGCCTCTTC ATGACCCTCC TACGAAAAAA GCAAGTACTG TTGGCATGTT CGGATCAATT 
TTGGGAAAAG CTGCTGCAGC AGTGCAATCA GCAACGCAAG CAGTCAATCA AAGTGCGTCG 
TCAGTGGCAT CAGTCGTTGC ACAAAAGCCA ACAATCGTAC CGAGAACGAA CAACGTGCTT 
TTGTTGTCTA GCGTATGCTC ACCTAACGAG ATTAAAAGAA ATTCAAGTTC AGTAGAATTT 
GATTCAGAAT ATGGATATCA AATGCCCGAT GTAGAGTCTC TATCAAGCCA TTACGCAAAT 
ACTGGCGGTG ATTATGATAA TAGTAATATG AAAATACATG AATTTGGTAC ATATGCAGAT 
GATAGACCAT ACGCAGATTA TCATACGAAT GGCAATCAAA GCCAATTTAA AGAAGAGGCG 
GTGATTCCTG GGGAACCTGA AGTAATTAAT ACAAACATAC TCCCGATTGG CCCGCAGGCC 
ACCGGCAAAA AACTTCCTAC CGTCAATGGT AAATCGGCGT TACTTATTAA ACAGATGCCG 
ACGGAGGTAT ATGATGATGA ATCAGATACA GATGAATTAG ATGTCAGTCC ATCAACCGGA 
AAAGTACCTA GCTATAGCAT TTATAGTGAG CAGGAAGATT ACTACATGGA TCTTCAGCAA 
ACGACACCGT CAATTCAGCC AAACGGTTTT TATGAACAAG TCAATAATGG TTATGACTAC 
CGAGAGGATT ACTTTAACGA GGAGGATGAA TATAAGTATC TCGAGCAGCA GAGGGAGCAA 
GAGGAACATA ATCAGCCGAA GAACAAGAAA TATCTGAAGC AGGCCAAAAT ATCAAAAATA 
CAACCACCAT CATTAGATTT CATTGACGTA GGGCAAGACG ATGACTTTAT ATACGACAAC 
TATCACAGCG AAGACGATAG CGGAAATTAC CTAGAGGGAA GCTCCAGTGG AAGCGTTGGT 
CCTATTGAAG GGTCAATCAT AAAGGTGGAC TCGAATATAG AAGCTTCGTT TGCTTCGCTC 
AACAAGAAGA GTGACTCTTT CACACCGACA AACGATTCCC TTCAAAAGCA TGACACTGTT 
ATTGGTGAAT CAACAACCAA ACTTACGCGC CTTAGGACAG AAAAAATGTG CCCAGACGTA 
GACGAAGAAG ATGAAAACCT AAGCGATCAC GTTTCCGATC TTACTGACTT AAGTAAACTA 
ATCTCACAAA AAAAAAAAAC GCTCTTGAGA GGAGAAACTG AAGAAGTCGT AGGAGGACAC 
ATGCAGGTGT TACGCCAAAC GGAAATCACA GCTCGGCAAC GATGGCATTG GGCTTACAAT 
AAAATAATCA TGCAGCTTAA TAATGGAGGT GGTCCTGGAG AAGTGGGATT ACGTACAAAT 
GGTCATCCCG GTGACAATCC ATTCTATAGC AATATTGACA GTATGCCGGA CATAAGGCCT 
CGAAGAAAAT CCATTCCTCT TGTGTCTGAG CTGGTTCTCA AGGCGAATTA TCCTAATGCG 
CCCTCGAAAA TGGATCAATG TTATCTCCTA AGTTTGCAAG AAAATGTCGA GACCACGACA 
ATGGCCGCTA CAAAACGAAA TGCTGGACTC ACATCTGCTG TTCCACGTGC AACGCTGAAT 
GACGAAGATC TGAAAATGCA CGTGTACAAA AAAGCTCTAC AAGCCCTGAT ATATCCTATA 
TCCTCAACAA CGCCCCACAA TTTTTTGTTA TGGACTGCTA CGTCACCAAC GTACTGCTAC 
GAGTGTGAGG GTCTTTTGTG GGGGATAGCA CGACAAGGGG TCCGTTGCAC TGAGTGCGGT 
GTAAAGTGTC ATGAAAAATG TAAGGACCTT CTGAATGCGG ACTGCCTGCA GCGTGCTGCC 
GAAAAAAGCT CAAAGCACGG TGCGGAAGAC AAGGCTAACT CGATTATTAC AGCGATGAAA 
GATCGTATGA AGCAACGTGA ACGAGAGAAG CCTGAAATTT TTGAGTTGAT TAGCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001259075.1
CDS	591..8726
Translation	MTHYVRHDYFHNTQNGALSSDTSRISYSQISYETQPSREYFSESYALSNQGPEECSRVSHLNSDTVLTTVDNSNNSYGYDYLECYGANIQCDPEEDSVDNWNENTSVVADQYGLGHNNLNCTSSKLLPKLPNIENGRGSSNACAPQMDVKFNTKDAAQRDGRGFDTDQTDAMGSESSTYEVYEKMQRPYTSMLPLDYSDYQEGCYNTDNLSTYSDTPPSNNTQLKRQMQRKISLMMAMTTASVIASGNTISTNAAARDLDRCLATESCEVIVDTRDSGSVTSFPSSAVTAITKTRKLPKVLPTPLCKSSRHPITIATDALSSSYTSDPLPEKSHRPKQLPKLPISLPQSNDRASLNSNWATPPAPDALPFNSFDHKSASSPTPTTTITKDTETTSYLVETDFIGARHNALYQYDSKEPNIVFSDKSVEAEHSPTWTPLSPIQSKQSPCPPVALPSNIMQNVSLTCHLPEIEATRSDIEREPESSSIEPILEIEKLADPYSGPGSALFNISEYLKPYTLNKPILSEEKKNHIANAASTSTTTPLNITSDDEFSSYSNKWTSTCVNFQPLDVESSLNISLKVNAGTNQAELLMTPLKSSTPLFISSNGTSDNFNLRKSSPPDSAFTTTVNVNSFETVLVSGSQTASPSPSNLKSPPSIAPLLSYSDYMKQFELPELPQPIMDLSENDTATQSDSFNVINNTLTNADNLNSYNQMDVESKSSLQLPSYSSESFDPCSVPSFSIKNKEYKIVEKLDSLSNVESVESPKTLVSPVNPLNCSKLLPGTESIVSNDVAFDDTFYDSFNVDIKELTAFVDHVAPEDGLYNFPNDKTSVEFSFDKTEDTIDMNQNLSSGECGYYKPSQAQQKASVVASAASSVLDGISKGLKGGLDGVFSGVSSTVDVTQSNPSSKRGFSFNLASKIVPSVGGLLTSTSSTSIKQTGSETNPTLILISPENVSSRNSNYIPTTSPSCTQKNGEENLYSATVHNKSTKSNSYYNEVGEISSTLVRNVCDSYDNSYDEMILTNEMVNIGMLDSESEFGLIENSYSYQVPDNEQIDSVNSYNNKTQNVTNNGIEKANTKNKPVPLHDPPTKKASTVGMFGSILGKAAAAVQSATQAVNQSASSVASVVAQKPTIVPRTNNVLLLSSVCSPNEIKRNSSSVEFDSEYGYQMPDVESLSSHYANTGGDYDNSNMKIHEFGTYADDRPYADYHTNGNQSQFKEEAVIPGEPEVINTNILPIGPQATGKKLPTVNGKSALLIKQMPTEVYDDESDTDELDVSPSTGKVPSYSIYSEQEDYYMDLQQTTPSIQPNGFYEQVNNGYDYREDYFNEEDEYKYLEQQREQEEHNQPKNKKYLKQAKISKIQPPSLDFIDVGQDDDFIYDNYHSEDDSGNYLEGSSSGSVGPIEGSIIKVDSNIEASFASLNKKSDSFTPTNDSLQKHDTVIGESTTKLTRLRTEKMCPDVDEEDENLSDHVSDLTDLSKLISQKKKTLLRGETEEVVGGHMQVLRQTEITARQRWHWAYNKIIMQLNNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELVLKANYPNAPSKMDQCYLLSLQENVETTTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELISECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_001272146.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant F (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001272146.1

ORF Insert Sequence:

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGACGCGGC TCAGAGGGAT 
GGACGCGGCT TCGACACAGA TCAAACTGAT GCGATGGGTT CTGAATCTAG TACATATGAG 
GTTTATGAAA AAATGCAAAG GCCATACACG TCCATGTTGC CGTTAGATTA CAGTGATTAC 
CAAGAAGGTT GTTACAATAC CGACAATCTT TCAACATACA GCGACACACC GCCGAGCAAT 
AATACACAAC TAAAGAGGCA AATGCAGAGG AAAATTTCGT TAATGATGGC GATGACTACA 
GCTTCTGTGA TAGCATCTGG AAACACCATC AGCACCAATG CCGCTGCAAG AGACTTAGAC 
AGATGTTTGG CGACGGAATC TTGTGAAGTA ATTGTCGATA CACGTGACTC TGGTAGCGTC 
ACAAGTTTCC CGTCATCTGC TGTGACTGCA ATAACAAAGA CTAGAAAATT GCCAAAGGTT 
TTACCAACGC CACTGTGTAA ATCATCTCGT CATCCAATCA CCATAGCCAC CGATGCATTA 
AGTTCATCAT ATACTTCAGA TCCTTTACCA GAGAAGAGTC ATCGCCCAAA GCAACTGCCA 
AAGCTGCCTA TATCATTACC CCAGTCCAAT GATCGTGCAA GTCTCAATTC GAATTGGGCG 
ACCCCTCCTG CCCCAGATGC ATTACCATTT AATTCGTTCG ACCATAAATC GGCATCTTCT 
CCTACGCCAA CAACAACAAT AACAAAAGAT ACGGAAACCA CCTCATATCT GGTTGAAACA 
GATTTTATTG GAGCCAGGCA TAATGCCCTT TATCAGTATG ATAGTAAAGA ACCAAATATA 
GTATTTTCGG ACAAGTCTGT CGAAGCAGAA CACTCGCCAA CGTGGACCCC ATTATCGCCA 
ATACAATCCA AACAAAGTCC TTGTCCGCCT GTGGCGCTAC CAAGCAATAT TATGCAAAAT 
GTTTCTCTAA CATGTCACTT ACCTGAAATC GAAGCAACCC GATCCGATAT TGAGCGAGAA 
CCAGAAAGCT CTTCTATCGA ACCAATTCTA GAAATTGAAA AATTAGCCGA TCCATATAGC 
GGGCCCGGAT CAGCCTTATT TAACATATCT GAGTATCTGA AGCCTTATAC ATTAAATAAA 
CCTATTTTGT CCGAAGAAAA GAAAAACCAT ATCGCCAATG CTGCCAGTAC GTCAACCACA 
ACGCCATTGA ATATAACATC AGATGACGAA TTCTCTTCAT ATTCTAACAA GTGGACGAGT 
ACTTGCGTTA ATTTTCAACC ATTAGACGTT GAATCAAGCT TAAATATTTC ACTTAAAGTC 
AACGCTGGCA CGAATCAGGC CGAATTATTA ATGACACCAT TAAAAAGTTC CACTCCTCTT 
TTCATTTCTT CTAATGGCAC ATCCGATAAT TTTAATTTGA GGAAAAGCTC TCCTCCCGAC 
AGCGCGTTCA CCACCACGGT TAACGTCAAC AGTTTTGAAA CGGTACTAGT ATCTGGAAGC 
CAAACTGCCT CACCGTCGCC GTCAAACTTA AAGTCACCAC CATCAATAGC TCCATTATTA 
TCCTACTCGG ATTATATGAA ACAATTTGAG TTACCAGAAT TGCCGCAACC CATTATGGAT 
CTTTCAGAAA ATGATACTGC TACTCAAAGC GATTCCTTTA ATGTAATCAA TAATACTCTT 
ACGAACGCTG ACAATTTAAA TTCCTACAAC CAGATGGATG TAGAAAGCAA AAGTAGTCTA 
CAGTTACCTT CTTATTCCAG TGAATCATTT GATCCGTGTA GTGTGCCTAG TTTTTCAATT 
AAGAACAAAG AATATAAAAT TGTCGAAAAG CTGGACAGTC TATCAAATGT TGAAAGCGTA 
GAATCACCGA AAACCTTAGT AAGTCCAGTA AACCCATTGA ATTGCTCAAA ATTATTGCCA 
GGGACGGAAT CAATAGTGAG TAATGATGTA GCGTTCGATG ATACATTTTA CGATAGCTTT 
AATGTTGACA TAAAAGAACT GACCGCATTT GTTGACCATG TTGCCCCAGA GGACGGTTTA 
TACAATTTCC CAAACGATAA GACTTCAGTT GAATTCAGTT TTGATAAGAC CGAGGATACA 
ATAGACATGA ATCAGAACTT AAGCTCAGGA GAATGTGGTT ATTATAAGCC ATCTCAGGCA 
CAACAAAAAG CTAGTGTAGT AGCATCCGCA GCAAGTAGTG TTCTTGACGG AATTTCAAAG 
GGCTTAAAAG GGGGGCTGGA CGGCGTTTTT AGTGGTGTCA GTTCAACAGT TGATGTCACT 
CAATCAAATC CCAGTTCCAA AAGAGGTTTT AGCTTTAACT TAGCATCAAA GATTGTGCCA 
AGTGTCGGTG GATTGCTAAC TAGTACAAGT TCCACCTCTA TAAAACAAAC AGGATCAGAA 
ACTAATCCTA CCCTAATATT GATTTCACCT GAAAATGTTT CTAGTCGTAA CAGTAATTAT 
ATACCTACGA CATCTCCATC TTGTACTCAA AAAAATGGCG AGGAAAATTT ATATTCGGCT 
ACAGTCCACA ATAAGTCAAC GAAATCGAAT TCATACTATA ATGAAGTGGG AGAAATTAGT 
TCGACCTTAG TCAGAAATGT TTGTGACAGT TACGATAATA GCTATGATGA GATGATTTTA 
ACCAACGAAA TGGTAAATAT AGGGATGCTC GACAGTGAAT CTGAGTTTGG CTTAATAGAA 
AATTCATATT CGTATCAGGT ACCAGACAAT GAGCAAATCG ACAGTGTTAA CTCGTATAAT 
AATAAGACCC AAAATGTAAC AAATAATGGC ATAGAAAAGG CAAATACAAA AAATAAACCA 
GTGCCTCTTC ATGACCCTCC TACGAAAAAA GCAAGTACTG TTGGCATGTT CGGATCAATT 
TTGGGAAAAG CTGCTGCAGC AGTGCAATCA GCAACGCAAG CAGTCAATCA AAGTGCGTCG 
TCAGTGGCAT CAGTCGTTGC ACAAAAGCCA ACAATCGTAC CGAGAACGAA CAACGTGCTT 
TTGTTGTCTA GCGTATGCTC ACCTAACGAG ATTAAAAGAA ATTCAAGTTC AGTAGAATTT 
GATTCAGAAT ATGGATATCA AATGCCCGAT GTAGAGTCTC TATCAAGCCA TTACGCAAAT 
ACTGGCGGTG ATTATGATAA TAGTAATATG AAAATACATG AATTTGGTAC ATATGCAGAT 
GATAGACCAT ACGCAGATTA TCATACGAAT GGCAATCAAA GCCAATTTAA AGAAGAGGCG 
GTGATTCCTG GGGAACCTGA AGTAATTAAT ACAAACATAC TCCCGATTGG CCCGCAGGCC 
ACCGGCAAAA AACTTCCTAC CGTCAATGGT AAATCGGCGT TACTTATTAA ACAGATGCCG 
ACGGAGGTAT ATGATGATGA ATCAGATACA GATGAATTAG ATGTCAGTCC ATCAACCGGA 
AAAGTACCTA GCTATAGCAT TTATAGTGAG CAGGAAGATT ACTACATGGA TCTTCAGCAA 
ACGACACCGT CAATTCAGCC AAACGGTTTT TATGAACAAG TCAATAATGG TTATGACTAC 
CGAGAGGATT ACTTTAACGA GGAGGATGAA TATAAGTATC TCGAGCAGCA GAGGGAGCAA 
GAGGAACATA ATCAGCCGAA GAACAAGAAA TATCTGAAGC AGGCCAAAAT ATCAAAAATA 
CAACCACCAT CATTAGATTT CATTGACGTA GGGCAAGACG ATGACTTTAT ATACGACAAC 
TATCACAGCG AAGACGATAG CGGAAATTAC CTAGAGGGAA GCTCCAGTGG AAGCGTTGGT 
CCTATTGAAG GGTCAATCAT AAAGGTGGAC TCGAATATAG AAGCTTCGTT TGCTTCGCTC 
AACAAGAAGA GTGACTCTTT CACACCGACA AACGATTCCC TTCAAAAGCA TGACACTGTT 
ATTGGTGAAT CAACAACCAA ACTTACGCGC CTTAGGACAG AAAAAATGTG CCCAGACGTA 
GACGAAGAAG ATGAAAACCT AAGCGATCAC GTTTCCGATC TTACTGACTT AAGTAAACTA 
ATCTCACAAA AAAAAAAAAC GCTCTTGAGA GGAGAAACTG AAGAAGTCGT AGGAGGACAC 
ATGCAGGTGT TACGCCAAAC GGAAATCACA GCTCGGCAAC GATGGCATTG GGCTTACAAT 
AAAATAATCA TGCAGCTTAA TAATGGAGGT GGTCCTGGAG AAGTGGGATT ACGTACAAAT 
GGTCATCCCG GTGACAATCC ATTCTATAGC AATATTGACA GTATGCCGGA CATAAGGCCT 
CGAAGAAAAT CCATTCCTCT TGTGTCTGAG CTGGTTCTCA AGGCGAATTA TCCTAATGCG 
CCCTCGAAAA TGGATCAATG TTATCTCCTA AGTTTGCAAG AAAATGTCGA GACCACGACA 
ATGGCCGCTA CAAAACGAAA TGCTGGACTC ACATCTGCTG TTCCACGTGC AACGCTGAAT 
GACGAAGATC TGAAAATGCA CGTGTACAAA AAAGCTCTAC AAGCCCTGAT ATATCCTATA 
TCCTCAACAA CGCCCCACAA TTTTTTGTTA TGGACTGCTA CGTCACCAAC GTACTGCTAC 
GAGTGTGAGG GTCTTTTGTG GGGGATAGCA CGACAAGGGG TCCGTTGCAC TGAGTGCGGT 
GTAAAGTGTC ATGAAAAATG TAAGGACCTT CTGAATGCGG ACTGCCTGCA GCGTGCTGCC 
GAAAAAAGCT CAAAGCACGG TGCGGAAGAC AAGGCTAACT CGATTATTAC AGCGATGAAA 
GATCGTATGA AGCAACGTGA ACGAGAGAAG CCTGAAATTT TTGAGTTGAT TAGCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-13 ( NM_001258498.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001245427.1 Drosophila melanogaster unc-13, transcript variant E (unc-13), mRNA.

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CloneID	OFa00318
Clone ID Related Accession (Same CDS sequence)	NM_001258498.2
Accession Version	NM_001258498.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9561bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). On Jan 16, 2013 this sequence version replaced NM_001258498.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATGAACA CATCTCAGCT GCAAGTGACC GGGGATACGG AAAAAAAAAG TCAGTTGCTT 
AAAAAGGAAC TCAAAATAAA CACCCAGGAA AAGCTTATCT TTGCAGAAAA TGCCCTCAAA 
AGCCAGATTA AAATAAAGGA GCAGTTACGA CTTCAGCAAC AATCAACGAT TTATGCGTCG 
TCACTATTGA GCTCTTCTGC TGCCGGATCA GTAAGAGCTC CGCTCTTAAG CCAGGGGCAC 
CTTAATTCTA TCCAACACAA CATGGATTTT GACCTTGCCA AAGCACAAAT ACCGGAAATG 
CAACCACCGA TGAGTAAGTC GCCCAATGGA TTGGATTTCT CTTACTTAAG TTATCCAAGC 
ATAAATACTA ATGAAAGTAT GATATCGATT AAAAGTGAAC AACAGTTGTG TCAGAGCTAC 
AATTCGGAGC AACACTCTGA CTACATTATC TCCGATTATA TGGATAAAAT TGCAACAAGG 
ATCAGTTTAC TCGAGACAGA GCTGAAGTTT GCTTGGCGTG CATTGGATTT GCTTTCTACG 
GAGTACGGAA AGATTTGGAT TCGTTTGGAA AAGCTTGAAA ACATATCAAT AGAACAACAG 
TCTGTCGTTG GCAACCTTGT GGATCTTATC GGCGCATCAA AAAAAGAACT TCAAAAAGTG 
GATATCGAAA GAATGAAAGT ACCCTTATAT CAAGATGAAG ATCAATTGCT ACCGTTGGAA 
ATGGAAGACA CATTAGATAT AGATATCCAA TCATCTAATC GTGACTTTGA TAAGAATCTT 
ACTTTTGAAA ATCATGAAAA AACTTTTGTA ACAAAACATA CGCAAGCTAC TAAATCGGAA 
GACCTTATGA ATTCCGCTTA CGCAATAGAT TCCCATCCAA ATTTTGAAAA CATTGATTTC 
AATGGCAAAA ACTTAGACAT TGGGATAATA AAATTTGGTT TTGAAAAAGG CTACGAGCCA 
AAACAAAAAG GTAATCAATC GGATTTTGAA GCATACAAGG AATCAAGAAC TAAACTTTAC 
TCAGATTCTG ACCTTATGTT ATATGAGAGG CAGCAGTTTC TAGCAAATAG CGCGCGTGCC 
GAATTAATGA AAGAATTTTT AAATGGACGT CGAGTTTTAA ATGAGATTTC GGCATCCTCG 
GGTGCATTGT CAAAAAGTTC TGAGAAGGAT AAAAATCTTG TAAAGCCTAG AGAACCACTG 
TTAAGTGAGA TAGATGATAC ATACGTGAGA CGGTCAACCT GTGAACTTGT CAGCCCTAGT 
AGTAATAATT TTTTTCAAAT AGCAGCCTCT AAAACAGGCG AGGGTGGAAA TCAGGTTTTA 
CCCATTGAAG ACACTGGAGG AACAGAAAGT CCAGCTAATA TTAATGAAGT AGACGAATCT 
TTTTATAAAA ATCTAAACGA GGCTTATCGA GACAATGAAT TGAGCTCTGA GATATTTAAG 
GTTGATGCAT TGCTACATCA GTCTGAGGCC ACACATGACC ACATCTCATT TATCCGAAAC 
CAGTCAACTT CTTCCCCAGT TTTAAATAAT CAGAGGGTGA ATGCTATTAC AGGTACACAA 
CCTTTACAGA CTGCAAAGGC GTGTCTCGTA ACAGAGCCAA ACCAACATTT GAACAAAGAA 
CCAAGAAAGC ACAGAAAAAA AAAACATCAT ACAAACGAAA TGGATATGAT TAATAAACTA 
AAGTGTATCC TTGCACAAGC ACAATCAACA GAGATTATTA AGAGAGACAT TGAAGAATTG 
GGTTCGAAAC TTTCAGATGC ATGTGTAAAA CAAACTGAAA CGGAAAATAA ATCAGACAAC 
CATATTAGTA CCAGTTATTT AAAAGATGAT ATACGAAAAA TGTTAAACAA CGTAATACAG 
ACTTTATTGG GGGAAATAAA TAAAATTAAA GGGTTACAAT CATTGAGTGC AACGCAATTG 
AGTCAGTTAC AAAATGCCGT ATGGTCGGAA GAAAGGTTTT TTCAAAAAAT TAGTCTGGTC 
GATAAAAAGT TGACTTTGCT GTTACTTAAT CCGTTAACTG TTACTGAAGA ACTGCAAAGG 
CTTTGCATTT CAAACACAAA TGAAAAGTTT GTTCTGGTAA TAAAAAAGTT AAAAAAAAAT 
ATTGACACTC TAAAAAAATT AGTTGGCAGC TCATTGGATG ACTTTAAAAT AGATAATTCA 
CACGCAAATA TGACCACATT TCACAGCCTT AATCCATCCC ATAATTCTAA TGATAGCAGT 
TTTAGTGCAC ATTTATTAAG AAATAACTCC AATCTGGATG AACAGTTGAA AATTCTTGAA 
ACTCAAGAGA TTGAAATACA CCGGAAAAAG AAAATAGACG AAATTGTGAG CGGACTTTCC 
GAAGAAACCT ATACTTTTAA CCCAGATATG GAATATAAAA GCCAACATAG TTGTACCAAC 
TCAACTGATA ATATTATAGG ATTCTCAAAA AATATTTACA ATGAAGATGA ATATATAAAG 
TCTTTAAGAA AGAGTCTAGA ACGCCACAAT AGCATGATCT TTTTACTACA TTTGCAAAAT 
CCTGAAAAAC ACAAAGTGTT GGCTGATATA AATGATGCAC AAATGGATTC AAATCGAGCT 
AGTCTCAGTC CTCCACCACC AGCTCCAACC GACACAGTTT ACTTAAATGA TGCAATTAAT 
CAAATTACAT ATCAACAACG AAATGGAAAA TCAGACTCTG GCCTATCGTC GATGAGCGGC 
TGGTCAGCCA ACTCCCAAGT GTCGGTTGGA CTTCAAAATT ATGATCCTGC ATGTAATAAT 
ATTTTGGAAA ATTGTAGCGA AAGATTGCAA ACATACCATT CATTTCCTTT GGCTGAAAAT 
TCGAACTTTC ACAGTTTCCC ACTCAGTCAT GAACAAGCTC AAACTACTAA TCAAACTGAG 
TTTGTAATAT TAGAAGAAAA TCTAAACTAT ATTTATGAGC TTTCTAAAAA TTTACCTATT 
TGCTCTGCCT ATGAAAATAA ATCTATTTTC GATATGAAAT ATGAAATTTG CGACTCAAAT 
GAAATTGGAA AATTTTCCAC TGTGGACGAA ATGCTGGAAT GGGACCAGCT AAATGAACCG 
GATAAACAAA ACTTTTCTGG GAAACGGTTA AACTCCAACC TTATGCCAGA TCTCTTGACT 
GCTCAAATAC CAAATTCCAT ATCAAAACAC AATAAAAACA CAAGTGTTAA CATTGAATAT 
GTTAGACAAA AAGAAAATAA AGGCATGGAT AGGCGAAGCA TCATAGAACC AAATATTTAT 
AACGGAAAAT CGGAAGATCA AATTTGCAGA CCTTGTTTGA CAGATAAACT CGTATTTTAT 
CCCTCATCGA ACTCTATTAC CGATCACAAT AGCAGCCATG ATTTCAATTG TTTGAGTCAA 
CAGGATCAAA CACGGATTAT TAAGGAATTC GGATCTGCGC ATTTGAATCA AGATCCAACA 
AACTATCTTG ATTATACGTC TGGTACTTAT TCTAAAGCGC CGCCAGAGGT CCTTACTCAC 
GAAACGAACT CAAGTCACCT GGAATTCAAT CATGAATCAG AGTCACTGTT CAACTCTCCT 
AATACTTCTA GCTATTGTAA ACAAAAGTTT GTTCCAGGAA CATCTCCAGC AAAGCCTTCA 
AAGGTATGGA AGAGGCTAAA CACCATATTA GCAGATAATC TAAAGCTCAA GCGTGTATCA 
AAGTTTAATA GATCACTGTC TTTACCCGGT GATGTGCAAA GTCAAGGCCT CCAGAGACAG 
CCTCGTGGCC AGGCCGGTTC GTGTCCGTTT ATCCACAAGC GATCCAATCT GGCCGGTAGC 
CCGGTTCAGT TATCGAAAAG AATTCAAAAG CTTCCCATAC GTTTTATAGG ACGAGCGAAA 
GGAGTCCCAT TCGTGCGGCG CTCATCAAGC CCGGACTCAG CTGTTTCATT AGATTCCGCT 
GCCGATAAAC GCTTCTCAAG CGAAAAAGGT TTAAAAAAAA AAACAATTTC GTCCAAGATG 
AGTGGTTTAA TGCAAAAGGC AAAGACGTAC AAACGGCACA GCTTTGTCCT GCGCCGCGGC 
TGTAACATGT CGGATTCTGA ATTAGAGATG CCGGACTTCG TTTCATCGGG TAACGATAAT 
AGTTCAATAT CGACAAGAGA AATTTTGTTG AATCAATCGA TCGAGGTAGA AGATGAACAA 
GAAGATTTTA ATTATAAAAA TCGGTGTGAC TCTAAAAGTG TGTTAGGTGG TTCAATTGAG 
AAGCTAAATG GCAACCTAAC AAATAATCTA TTCCCCATTG TAGGTGACCT AAAAAAAATT 
CAAAGCCCCT TACCACTGGC TGTCTTAACA GAAATTCCTT CCTATAAGGA TGAATATAGC 
AATAAATCCG ATAGTATCAA AAATTCACCC ATTGAAATGC CAAAGATTCT CTTAGAAACG 
GCATGCAATC AAGAACTCAA TTTAGCACAT TCTGACGACG ATGTTGATAA GAACATACTG 
GCAAACTCGG CCAAAGACTA TGTAAATGCC CCCACATTTA GTATATTAAA GACAGTTGAA 
GATGCTTCCG AACCAACGAT GACACCACTC CATACAACAA CAACGACAAA CAGCAGTCTT 
AATGTCACAA GCGCTTTATG GGTCACGCAG CAGTGCTTAG ACCTTCCAAA CTATCCCGGG 
TGGGGGTCGC GAGAAGATGA CGATAACCGC AGTCAGCATT CTGCTCGCAC TCTATCTTCT 
TCTCGTCGAC AAAGCACTGA GGATAGCATT GATACAGACG ACGAATACTT TTATTATGAG 
CTTCGCCAGT TAGAAGAGCA AGAAAAGCAG AGGGCGCACA ATTCTGCAAT TCCATCTTGT 
GAACGACAAA ATGATAATGA TGTGTTGTTT TCACAAATTG GACAACTTCT TCAAAACGAT 
GTTAACGGAG GAGACGGTTT TCGCCATTCT AATGGGTGTA ATGATGGGGA AGACGCTATT 
ATTTTTTCTC CAAGTGAAAG TGTTAAGTTA CGAATGTCTG AAGTGTTTAA GGAACTGAAA 
AGCGTGGTTA GTTTAAACCC AAGCGTTAAT AATGACGCGA CTTTTGAGGG CGTTCCTATT 
GTAAAACCAA CATATGAAAA ACTTGAGACA GTCTCCGACT TGCATAGCGC TTGGCAGGAT 
GTTAATGGTG ATTTACAAAT TGCAGCTTCA GACATCGATT CTAATGAAGA TCTAGTTGGA 
AATAAGGGTA GGGAAACTCC AACTTATAAC AAACAGCGAA AATTGAGACG GTTAAAGAAA 
AAAACTCGCG ATCGTAAAAT AAATATATCT AAGAATGCAA CCAGTTCCAG TTCCTCTTGC 
CATTCAGAAA ATGAATGTAA TACACCCCTT GGTCAATGTA CCCAGAAATC GGTGGCAGAA 
AAAGATACAA ATGATATATC AAATAAATCG GAGGCTTCTA GCGAGACATC AGGACCAGAT 
ACGCCGGCAG AGCTTAGCGA TGTTGATATA AGCGAAACTG AAAACGGTTT GAGGGCTGAC 
GACGGCCAGA ATATAGATAA CATGCGTGGA AATTCAGGGT CTCTTTTAAA GGTGAATCGG 
AAGTACCTAT TACAATTTGA TGTTGACCAT AGTTTAATAA ACCAGCCCCT GGAAACATCT 
CAGTATAATA CGCACATGTT GGAGAATATT ACAAGTGCGT CAATTCCATC GCAGAATCGC 
CAAATAGACT CAAAAACGTT AATGAGTCAA AGCTCACATG CGGATGGATC CCAGGCTGTA 
GGCAATGAAA CCGCTGTAGG TCTAAGCAGT AGCAAGTGGA AATTGTTAAA AACTTTAAAA 
GAGCGCAAGA TCGAGGAAAA AAATAATCAA GATAAAATAA AAGAAGATGA AATGATAAAG 
GACAGGGACA AGAATGGAGG TGGTCCTGGA GAAGTGGGAT TACGTACAAA TGGTCATCCC 
GGTGACAATC CATTCTATAG CAATATTGAC AGTATGCCGG ACATAAGGCC TCGAAGAAAA 
TCCATTCCTC TTGTGTCTGA GCTGGTTCTC AAGACAATGG CCGCTACAAA ACGAAATGCT 
GGACTCACAT CTGCTGTTCC ACGTGCAACG CTGAATGACG AAGATCTGAA AATGCACGTG 
TACAAAAAAG CTCTACAAGC CCTGATATAT CCTATATCCT CAACAACGCC CCACAATTTT 
TTGTTATGGA CTGCTACGTC ACCAACGTAC TGCTACGAGT GTGAGGGTCT TTTGTGGGGG 
ATAGCACGAC AAGGGGTCCG TTGCACTGAG TGCGGTGTAA AGTGTCATGA AAAATGTAAG 
GACCTTCTGA ATGCGGACTG CCTGCAGCGT GCTGCCGAAA AAAGCTCAAA GCACGGTGCG 
GAAGACAAGG CTAACTCGAT TATTACAGCG ATGAAAGATC GTATGAAGCA ACGTGAACGA 
GAGAAGCCTG AAATTTTTGA GTTGATTAGA GCCGTGTTCA GCGTTGAGGA GAAGAGTCAT 
GCCGGTCATA TGAAGGCTGT GAAGCAAAGC GTCCTGGATG GTACAAGCAA GTGGTCGGCC 
AAAATAGCAA TCACAGTGAT TTGTGCTCAA GGCCTGATAG CGAAGGATAA AAGCGGTACC 
AGTGATCCAT ATGTGACGGT GCAAGTTAGC AAGGTGAAAA AACGAACGCG AACAATGCCG 
CAGGAACTTA ATCCTGTGTG GAACGAGAAG TTTCACTTCG AGTGCCACAA CTCCTCCGAC 
CGCATTAAGG TTCGTGTTTG GGACGAAGAC AATGACCTAA AGTCGAAACT GCGCCAGAAG 
TTAACTCGAG AGTCGGACGA CTTTTTGGGA CAAACAATTA TCGAGGTTCG TACTCTTTCC 
GGTGAAATGG ATGTATGGTA CAACCTGGAA AAGCGCACAG ATAAATCGGC CGTATCAGGC 
GCGATACGGC TTCACATATC TGTTGAAATA AAGGGCGAGG AAAAAGTAGC TCCTTACCAC 
GTACAATATA CCTGTTTACA CGAGAACCTC TTCCATTATC TTTGCGAAGA AAATACCGGA 
ATGGTCAAGT TGCCAACTCA AAAAGGGGAC GATGCTTGGA AGTTGTATTT TGATGAAATA 
CCCGAAGAGA TTGTCGACGA GTTTTCGATG CGTTATGGAA TTGAGAATAT TTATCAAGCT 
ATGACACATT TCCACTGCTT GTCTGCTAAA TACCTATGTC CTGGGGTACC AGCTGTAATG 
AGTACCCTTT TGGCAAACAT AAATGCTTAT TATGCTCATA CGACTGCTTC GTCAGCTGTA 
TCGGCCAGTG ATAGATTTGC TGCCAGCAAT TTTGGGAAAG AAAAATTTGT TAAGTTGCTG 
GACCAGTTGC ATAACTCACT GAGGATCGAT TTGTCAATGT ACAGAAATAA CTTTCCTGCA 
TCGAGTCCAG AAAAGCTAAT GGATCTAAAA TCTACAGTTG ATTTATTGAC CAGCATAACA 
TTTTTCCGCA TGAAGGTACA AGAGCTATCT AGCCCGCCGA GAGCAAGTAC AGTAGTCAAG 
GATTGTGTTA AAGCATGCCT TAGATCCACT TATCAATTCT TATTTGAAAA TTGTTATGAA 
CTGTACAACA GAGAATTTCA GGTTGATCCC AACGAAGCAA AACGTGCACC AGATGATCAT 
GAACCCAAGC TTGATAGCGT CGACTTTTGG CATAAGCTAA TAGCTTTAAT CGTATCGGTA 
ATAGATGAAG ACAAAAACAG TTACGGAACC GTTTTAAATC AATTTCCGCA AGAGCTAAAT 
ATTGGACAGT TATCAGCATC TTCAATGTGG CATCTCTTTG CTGTGGACAT GAAGTACGCG 
CTCGAGGAGC ATGAGCAACA TCGTCTTTGC AAGTCATCGG CTTATATGAA TCTTCACTTT 
CGAGTTAAGT GGCTATATAG TAACTACGTC AAAGAGGTGC CTCCCTACAA AGGAGCGGTT 
CCCGATTACC CAGCATGGTT CGAGCCTTTT GTGATGCAGT GGCTAAATGA AAATGACGAC 
GTGTCATTGG AATACCTTCA TGGCGCATTT AAGAGGGATA AAAAAGACGG GTTTCAAAAG 
AGCAGCGAGC ACGCGCTCTT TTCAAATTCT GTAGTCGACG TTTTTACGCA ATTGACGCAG 
TGTTTCGATG TTGTTAGCAA ACTCGAGTGT CCTGATCCAG AAATTTGGAA ACGTTACATG 
AGACGTTTTG CTAAAACAAT CGTAAAAGTG CTAATAGCCT ATGCGGATAT TGTGAAACTT 
GAGTTTCCAG AGCATATGAA GGATGAAAGA ATTGCTTGCA TACTTATGAA CAACATTCAG 
CAACTAAGGG TGCAGCTAGA AAAAATGTTT GAGTCTATGG GTGGGGATAA GCTAGAGGAG 
GATGCAGCCA ATATTTTGAA AGAACTTCAG CAAAATCTTA ACTCTGCGTT GGACGACTTA 
GCTTCACAAT TCGCTATCAG TTTGGAGCCA CGAATAACTC AATCAGTGCG AGAACTTGGA 
GACATGCTGC TTTCTATTAA AGGAGGCAGT GGAACCCTTG CTGCTGGTAA TCTTGCAGCT 
CAAAGGAACG CGGTCGCCGT TGAGGCCGAT GAAGTTTTAA GGCCGCTTAT GGATTTACTT 
GATGGCTCTT TAACTCTCTA TGCGCAATCA TGTGAAAAAA CCGTGTTGAA GAGGTTACTA 
AAGGAGTTAT GGAAGATCGT AATGCGAATA TTAGAGAAAA CAATTGTTTT ACCACCTATG 
ACTGATAAAA CTATGATGTT TAAACACCTT ACTGACAATG CAAAAAACTT GGCATCGAAT 
GCAAAAATTG AAGACATGGG TCGTTTGTTC AAGTCACATA TGGCAGGAAA GCAGGATGTT 
AAGAGTGCTC TGAGCGGGGT AATGGACATA TCAAAAGAAG TAGAAAAAAA CTTGTCACCC 
AAGCAATGCG CAGTGCTTGA TGTTGCCTTA GATACAATAA AACAATACTT TCACGCGGGT 
GGCAATGGAC TGAAAAAGAC CTTTCTCGAA AAGTCGCCCG AGCTTCAGAG TTTACGTTAC 
GCACTCAGTT TATATACACA AATGACCGAC ACACTAATCA AAACCTTTAT ATCTAGCCAA 
GTTCACGAGG TCGATCTAGA AAACTCAGAG GAAAGTGTTG GGGAAATATC CGTGCAAATA 
GACTTATTTT CCCATCCCGG CACCGGTGAA CATAAAGTAA ACGTGAAAGT TGTTGCTGCT 
AATGACCTTA AATGGCAAAT ACCATCGGGT ATGTTCAGAC CATTTGTTGA CATTAATTTA 
ATAGGACCGC ACCTGCAAGA AAAAAAAAGG AAGTTCGCTA CAAAGTCGAA GTCTAACAAC 
TGGTCGCCTA AATACAACGA ATCTTTCAGC TTCACCATTG GAAATGAGGA GCAGCTGGAC 
TTCTTTGAAT TGCACATTTG CGTTAAGGAC TATTGTTTTG CTCGTGATGA CCGACTTGTG 
GGCGTGGCTG TAATTCCATT AAAAGATATA TCGGAAAAGG GATCGGTCGC ATGCTGGTTA 
CCCTTAATGA GACGAATTGA AATGGACGAA ACGGGCTGGA CCATTTTAAG AATTTTGTCA 
CAGCGAAATA ATGATGAAGT GGCCAAGGAA TTTGTCAAGC TTAAATCGGA AATACGGCAG 
GAGCCAACCA TGGGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001245427.1
CDS	162..9722
Translation	MMNTSQLQVTGDTEKKSQLLKKELKINTQEKLIFAENALKSQIKIKEQLRLQQQSTIYASSLLSSSAAGSVRAPLLSQGHLNSIQHNMDFDLAKAQIPEMQPPMSKSPNGLDFSYLSYPSINTNESMISIKSEQQLCQSYNSEQHSDYIISDYMDKIATRISLLETELKFAWRALDLLSTEYGKIWIRLEKLENISIEQQSVVGNLVDLIGASKKELQKVDIERMKVPLYQDEDQLLPLEMEDTLDIDIQSSNRDFDKNLTFENHEKTFVTKHTQATKSEDLMNSAYAIDSHPNFENIDFNGKNLDIGIIKFGFEKGYEPKQKGNQSDFEAYKESRTKLYSDSDLMLYERQQFLANSARAELMKEFLNGRRVLNEISASSGALSKSSEKDKNLVKPREPLLSEIDDTYVRRSTCELVSPSSNNFFQIAASKTGEGGNQVLPIEDTGGTESPANINEVDESFYKNLNEAYRDNELSSEIFKVDALLHQSEATHDHISFIRNQSTSSPVLNNQRVNAITGTQPLQTAKACLVTEPNQHLNKEPRKHRKKKHHTNEMDMINKLKCILAQAQSTEIIKRDIEELGSKLSDACVKQTETENKSDNHISTSYLKDDIRKMLNNVIQTLLGEINKIKGLQSLSATQLSQLQNAVWSEERFFQKISLVDKKLTLLLLNPLTVTEELQRLCISNTNEKFVLVIKKLKKNIDTLKKLVGSSLDDFKIDNSHANMTTFHSLNPSHNSNDSSFSAHLLRNNSNLDEQLKILETQEIEIHRKKKIDEIVSGLSEETYTFNPDMEYKSQHSCTNSTDNIIGFSKNIYNEDEYIKSLRKSLERHNSMIFLLHLQNPEKHKVLADINDAQMDSNRASLSPPPPAPTDTVYLNDAINQITYQQRNGKSDSGLSSMSGWSANSQVSVGLQNYDPACNNILENCSERLQTYHSFPLAENSNFHSFPLSHEQAQTTNQTEFVILEENLNYIYELSKNLPICSAYENKSIFDMKYEICDSNEIGKFSTVDEMLEWDQLNEPDKQNFSGKRLNSNLMPDLLTAQIPNSISKHNKNTSVNIEYVRQKENKGMDRRSIIEPNIYNGKSEDQICRPCLTDKLVFYPSSNSITDHNSSHDFNCLSQQDQTRIIKEFGSAHLNQDPTNYLDYTSGTYSKAPPEVLTHETNSSHLEFNHESESLFNSPNTSSYCKQKFVPGTSPAKPSKVWKRLNTILADNLKLKRVSKFNRSLSLPGDVQSQGLQRQPRGQAGSCPFIHKRSNLAGSPVQLSKRIQKLPIRFIGRAKGVPFVRRSSSPDSAVSLDSAADKRFSSEKGLKKKTISSKMSGLMQKAKTYKRHSFVLRRGCNMSDSELEMPDFVSSGNDNSSISTREILLNQSIEVEDEQEDFNYKNRCDSKSVLGGSIEKLNGNLTNNLFPIVGDLKKIQSPLPLAVLTEIPSYKDEYSNKSDSIKNSPIEMPKILLETACNQELNLAHSDDDVDKNILANSAKDYVNAPTFSILKTVEDASEPTMTPLHTTTTTNSSLNVTSALWVTQQCLDLPNYPGWGSREDDDNRSQHSARTLSSSRRQSTEDSIDTDDEYFYYELRQLEEQEKQRAHNSAIPSCERQNDNDVLFSQIGQLLQNDVNGGDGFRHSNGCNDGEDAIIFSPSESVKLRMSEVFKELKSVVSLNPSVNNDATFEGVPIVKPTYEKLETVSDLHSAWQDVNGDLQIAASDIDSNEDLVGNKGRETPTYNKQRKLRRLKKKTRDRKINISKNATSSSSSCHSENECNTPLGQCTQKSVAEKDTNDISNKSEASSETSGPDTPAELSDVDISETENGLRADDGQNIDNMRGNSGSLLKVNRKYLLQFDVDHSLINQPLETSQYNTHMLENITSASIPSQNRQIDSKTLMSQSSHADGSQAVGNETAVGLSSSKWKLLKTLKERKIEEKNNQDKIKEDEMIKDRDKNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELVLKTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_001258498.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant E (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001258498.2

ORF Insert Sequence:

ATGATGAACA CATCTCAGCT GCAAGTGACC GGGGATACGG AAAAAAAAAG TCAGTTGCTT 
AAAAAGGAAC TCAAAATAAA CACCCAGGAA AAGCTTATCT TTGCAGAAAA TGCCCTCAAA 
AGCCAGATTA AAATAAAGGA GCAGTTACGA CTTCAGCAAC AATCAACGAT TTATGCGTCG 
TCACTATTGA GCTCTTCTGC TGCCGGATCA GTAAGAGCTC CGCTCTTAAG CCAGGGGCAC 
CTTAATTCTA TCCAACACAA CATGGATTTT GACCTTGCCA AAGCACAAAT ACCGGAAATG 
CAACCACCGA TGAGTAAGTC GCCCAATGGA TTGGATTTCT CTTACTTAAG TTATCCAAGC 
ATAAATACTA ATGAAAGTAT GATATCGATT AAAAGTGAAC AACAGTTGTG TCAGAGCTAC 
AATTCGGAGC AACACTCTGA CTACATTATC TCCGATTATA TGGATAAAAT TGCAACAAGG 
ATCAGTTTAC TCGAGACAGA GCTGAAGTTT GCTTGGCGTG CATTGGATTT GCTTTCTACG 
GAGTACGGAA AGATTTGGAT TCGTTTGGAA AAGCTTGAAA ACATATCAAT AGAACAACAG 
TCTGTCGTTG GCAACCTTGT GGATCTTATC GGCGCATCAA AAAAAGAACT TCAAAAAGTG 
GATATCGAAA GAATGAAAGT ACCCTTATAT CAAGATGAAG ATCAATTGCT ACCGTTGGAA 
ATGGAAGACA CATTAGATAT AGATATCCAA TCATCTAATC GTGACTTTGA TAAGAATCTT 
ACTTTTGAAA ATCATGAAAA AACTTTTGTA ACAAAACATA CGCAAGCTAC TAAATCGGAA 
GACCTTATGA ATTCCGCTTA CGCAATAGAT TCCCATCCAA ATTTTGAAAA CATTGATTTC 
AATGGCAAAA ACTTAGACAT TGGGATAATA AAATTTGGTT TTGAAAAAGG CTACGAGCCA 
AAACAAAAAG GTAATCAATC GGATTTTGAA GCATACAAGG AATCAAGAAC TAAACTTTAC 
TCAGATTCTG ACCTTATGTT ATATGAGAGG CAGCAGTTTC TAGCAAATAG CGCGCGTGCC 
GAATTAATGA AAGAATTTTT AAATGGACGT CGAGTTTTAA ATGAGATTTC GGCATCCTCG 
GGTGCATTGT CAAAAAGTTC TGAGAAGGAT AAAAATCTTG TAAAGCCTAG AGAACCACTG 
TTAAGTGAGA TAGATGATAC ATACGTGAGA CGGTCAACCT GTGAACTTGT CAGCCCTAGT 
AGTAATAATT TTTTTCAAAT AGCAGCCTCT AAAACAGGCG AGGGTGGAAA TCAGGTTTTA 
CCCATTGAAG ACACTGGAGG AACAGAAAGT CCAGCTAATA TTAATGAAGT AGACGAATCT 
TTTTATAAAA ATCTAAACGA GGCTTATCGA GACAATGAAT TGAGCTCTGA GATATTTAAG 
GTTGATGCAT TGCTACATCA GTCTGAGGCC ACACATGACC ACATCTCATT TATCCGAAAC 
CAGTCAACTT CTTCCCCAGT TTTAAATAAT CAGAGGGTGA ATGCTATTAC AGGTACACAA 
CCTTTACAGA CTGCAAAGGC GTGTCTCGTA ACAGAGCCAA ACCAACATTT GAACAAAGAA 
CCAAGAAAGC ACAGAAAAAA AAAACATCAT ACAAACGAAA TGGATATGAT TAATAAACTA 
AAGTGTATCC TTGCACAAGC ACAATCAACA GAGATTATTA AGAGAGACAT TGAAGAATTG 
GGTTCGAAAC TTTCAGATGC ATGTGTAAAA CAAACTGAAA CGGAAAATAA ATCAGACAAC 
CATATTAGTA CCAGTTATTT AAAAGATGAT ATACGAAAAA TGTTAAACAA CGTAATACAG 
ACTTTATTGG GGGAAATAAA TAAAATTAAA GGGTTACAAT CATTGAGTGC AACGCAATTG 
AGTCAGTTAC AAAATGCCGT ATGGTCGGAA GAAAGGTTTT TTCAAAAAAT TAGTCTGGTC 
GATAAAAAGT TGACTTTGCT GTTACTTAAT CCGTTAACTG TTACTGAAGA ACTGCAAAGG 
CTTTGCATTT CAAACACAAA TGAAAAGTTT GTTCTGGTAA TAAAAAAGTT AAAAAAAAAT 
ATTGACACTC TAAAAAAATT AGTTGGCAGC TCATTGGATG ACTTTAAAAT AGATAATTCA 
CACGCAAATA TGACCACATT TCACAGCCTT AATCCATCCC ATAATTCTAA TGATAGCAGT 
TTTAGTGCAC ATTTATTAAG AAATAACTCC AATCTGGATG AACAGTTGAA AATTCTTGAA 
ACTCAAGAGA TTGAAATACA CCGGAAAAAG AAAATAGACG AAATTGTGAG CGGACTTTCC 
GAAGAAACCT ATACTTTTAA CCCAGATATG GAATATAAAA GCCAACATAG TTGTACCAAC 
TCAACTGATA ATATTATAGG ATTCTCAAAA AATATTTACA ATGAAGATGA ATATATAAAG 
TCTTTAAGAA AGAGTCTAGA ACGCCACAAT AGCATGATCT TTTTACTACA TTTGCAAAAT 
CCTGAAAAAC ACAAAGTGTT GGCTGATATA AATGATGCAC AAATGGATTC AAATCGAGCT 
AGTCTCAGTC CTCCACCACC AGCTCCAACC GACACAGTTT ACTTAAATGA TGCAATTAAT 
CAAATTACAT ATCAACAACG AAATGGAAAA TCAGACTCTG GCCTATCGTC GATGAGCGGC 
TGGTCAGCCA ACTCCCAAGT GTCGGTTGGA CTTCAAAATT ATGATCCTGC ATGTAATAAT 
ATTTTGGAAA ATTGTAGCGA AAGATTGCAA ACATACCATT CATTTCCTTT GGCTGAAAAT 
TCGAACTTTC ACAGTTTCCC ACTCAGTCAT GAACAAGCTC AAACTACTAA TCAAACTGAG 
TTTGTAATAT TAGAAGAAAA TCTAAACTAT ATTTATGAGC TTTCTAAAAA TTTACCTATT 
TGCTCTGCCT ATGAAAATAA ATCTATTTTC GATATGAAAT ATGAAATTTG CGACTCAAAT 
GAAATTGGAA AATTTTCCAC TGTGGACGAA ATGCTGGAAT GGGACCAGCT AAATGAACCG 
GATAAACAAA ACTTTTCTGG GAAACGGTTA AACTCCAACC TTATGCCAGA TCTCTTGACT 
GCTCAAATAC CAAATTCCAT ATCAAAACAC AATAAAAACA CAAGTGTTAA CATTGAATAT 
GTTAGACAAA AAGAAAATAA AGGCATGGAT AGGCGAAGCA TCATAGAACC AAATATTTAT 
AACGGAAAAT CGGAAGATCA AATTTGCAGA CCTTGTTTGA CAGATAAACT CGTATTTTAT 
CCCTCATCGA ACTCTATTAC CGATCACAAT AGCAGCCATG ATTTCAATTG TTTGAGTCAA 
CAGGATCAAA CACGGATTAT TAAGGAATTC GGATCTGCGC ATTTGAATCA AGATCCAACA 
AACTATCTTG ATTATACGTC TGGTACTTAT TCTAAAGCGC CGCCAGAGGT CCTTACTCAC 
GAAACGAACT CAAGTCACCT GGAATTCAAT CATGAATCAG AGTCACTGTT CAACTCTCCT 
AATACTTCTA GCTATTGTAA ACAAAAGTTT GTTCCAGGAA CATCTCCAGC AAAGCCTTCA 
AAGGTATGGA AGAGGCTAAA CACCATATTA GCAGATAATC TAAAGCTCAA GCGTGTATCA 
AAGTTTAATA GATCACTGTC TTTACCCGGT GATGTGCAAA GTCAAGGCCT CCAGAGACAG 
CCTCGTGGCC AGGCCGGTTC GTGTCCGTTT ATCCACAAGC GATCCAATCT GGCCGGTAGC 
CCGGTTCAGT TATCGAAAAG AATTCAAAAG CTTCCCATAC GTTTTATAGG ACGAGCGAAA 
GGAGTCCCAT TCGTGCGGCG CTCATCAAGC CCGGACTCAG CTGTTTCATT AGATTCCGCT 
GCCGATAAAC GCTTCTCAAG CGAAAAAGGT TTAAAAAAAA AAACAATTTC GTCCAAGATG 
AGTGGTTTAA TGCAAAAGGC AAAGACGTAC AAACGGCACA GCTTTGTCCT GCGCCGCGGC 
TGTAACATGT CGGATTCTGA ATTAGAGATG CCGGACTTCG TTTCATCGGG TAACGATAAT 
AGTTCAATAT CGACAAGAGA AATTTTGTTG AATCAATCGA TCGAGGTAGA AGATGAACAA 
GAAGATTTTA ATTATAAAAA TCGGTGTGAC TCTAAAAGTG TGTTAGGTGG TTCAATTGAG 
AAGCTAAATG GCAACCTAAC AAATAATCTA TTCCCCATTG TAGGTGACCT AAAAAAAATT 
CAAAGCCCCT TACCACTGGC TGTCTTAACA GAAATTCCTT CCTATAAGGA TGAATATAGC 
AATAAATCCG ATAGTATCAA AAATTCACCC ATTGAAATGC CAAAGATTCT CTTAGAAACG 
GCATGCAATC AAGAACTCAA TTTAGCACAT TCTGACGACG ATGTTGATAA GAACATACTG 
GCAAACTCGG CCAAAGACTA TGTAAATGCC CCCACATTTA GTATATTAAA GACAGTTGAA 
GATGCTTCCG AACCAACGAT GACACCACTC CATACAACAA CAACGACAAA CAGCAGTCTT 
AATGTCACAA GCGCTTTATG GGTCACGCAG CAGTGCTTAG ACCTTCCAAA CTATCCCGGG 
TGGGGGTCGC GAGAAGATGA CGATAACCGC AGTCAGCATT CTGCTCGCAC TCTATCTTCT 
TCTCGTCGAC AAAGCACTGA GGATAGCATT GATACAGACG ACGAATACTT TTATTATGAG 
CTTCGCCAGT TAGAAGAGCA AGAAAAGCAG AGGGCGCACA ATTCTGCAAT TCCATCTTGT 
GAACGACAAA ATGATAATGA TGTGTTGTTT TCACAAATTG GACAACTTCT TCAAAACGAT 
GTTAACGGAG GAGACGGTTT TCGCCATTCT AATGGGTGTA ATGATGGGGA AGACGCTATT 
ATTTTTTCTC CAAGTGAAAG TGTTAAGTTA CGAATGTCTG AAGTGTTTAA GGAACTGAAA 
AGCGTGGTTA GTTTAAACCC AAGCGTTAAT AATGACGCGA CTTTTGAGGG CGTTCCTATT 
GTAAAACCAA CATATGAAAA ACTTGAGACA GTCTCCGACT TGCATAGCGC TTGGCAGGAT 
GTTAATGGTG ATTTACAAAT TGCAGCTTCA GACATCGATT CTAATGAAGA TCTAGTTGGA 
AATAAGGGTA GGGAAACTCC AACTTATAAC AAACAGCGAA AATTGAGACG GTTAAAGAAA 
AAAACTCGCG ATCGTAAAAT AAATATATCT AAGAATGCAA CCAGTTCCAG TTCCTCTTGC 
CATTCAGAAA ATGAATGTAA TACACCCCTT GGTCAATGTA CCCAGAAATC GGTGGCAGAA 
AAAGATACAA ATGATATATC AAATAAATCG GAGGCTTCTA GCGAGACATC AGGACCAGAT 
ACGCCGGCAG AGCTTAGCGA TGTTGATATA AGCGAAACTG AAAACGGTTT GAGGGCTGAC 
GACGGCCAGA ATATAGATAA CATGCGTGGA AATTCAGGGT CTCTTTTAAA GGTGAATCGG 
AAGTACCTAT TACAATTTGA TGTTGACCAT AGTTTAATAA ACCAGCCCCT GGAAACATCT 
CAGTATAATA CGCACATGTT GGAGAATATT ACAAGTGCGT CAATTCCATC GCAGAATCGC 
CAAATAGACT CAAAAACGTT AATGAGTCAA AGCTCACATG CGGATGGATC CCAGGCTGTA 
GGCAATGAAA CCGCTGTAGG TCTAAGCAGT AGCAAGTGGA AATTGTTAAA AACTTTAAAA 
GAGCGCAAGA TCGAGGAAAA AAATAATCAA GATAAAATAA AAGAAGATGA AATGATAAAG 
GACAGGGACA AGAATGGAGG TGGTCCTGGA GAAGTGGGAT TACGTACAAA TGGTCATCCC 
GGTGACAATC CATTCTATAG CAATATTGAC AGTATGCCGG ACATAAGGCC TCGAAGAAAA 
TCCATTCCTC TTGTGTCTGA GCTGGTTCTC AAGACAATGG CCGCTACAAA ACGAAATGCT 
GGACTCACAT CTGCTGTTCC ACGTGCAACG CTGAATGACG AAGATCTGAA AATGCACGTG 
TACAAAAAAG CTCTACAAGC CCTGATATAT CCTATATCCT CAACAACGCC CCACAATTTT 
TTGTTATGGA CTGCTACGTC ACCAACGTAC TGCTACGAGT GTGAGGGTCT TTTGTGGGGG 
ATAGCACGAC AAGGGGTCCG TTGCACTGAG TGCGGTGTAA AGTGTCATGA AAAATGTAAG 
GACCTTCTGA ATGCGGACTG CCTGCAGCGT GCTGCCGAAA AAAGCTCAAA GCACGGTGCG 
GAAGACAAGG CTAACTCGAT TATTACAGCG ATGAAAGATC GTATGAAGCA ACGTGAACGA 
GAGAAGCCTG AAATTTTTGA GTTGATTAGA GCCGTGTTCA GCGTTGAGGA GAAGAGTCAT 
GCCGGTCATA TGAAGGCTGT GAAGCAAAGC GTCCTGGATG GTACAAGCAA GTGGTCGGCC 
AAAATAGCAA TCACAGTGAT TTGTGCTCAA GGCCTGATAG CGAAGGATAA AAGCGGTACC 
AGTGATCCAT ATGTGACGGT GCAAGTTAGC AAGGTGAAAA AACGAACGCG AACAATGCCG 
CAGGAACTTA ATCCTGTGTG GAACGAGAAG TTTCACTTCG AGTGCCACAA CTCCTCCGAC 
CGCATTAAGG TTCGTGTTTG GGACGAAGAC AATGACCTAA AGTCGAAACT GCGCCAGAAG 
TTAACTCGAG AGTCGGACGA CTTTTTGGGA CAAACAATTA TCGAGGTTCG TACTCTTTCC 
GGTGAAATGG ATGTATGGTA CAACCTGGAA AAGCGCACAG ATAAATCGGC CGTATCAGGC 
GCGATACGGC TTCACATATC TGTTGAAATA AAGGGCGAGG AAAAAGTAGC TCCTTACCAC 
GTACAATATA CCTGTTTACA CGAGAACCTC TTCCATTATC TTTGCGAAGA AAATACCGGA 
ATGGTCAAGT TGCCAACTCA AAAAGGGGAC GATGCTTGGA AGTTGTATTT TGATGAAATA 
CCCGAAGAGA TTGTCGACGA GTTTTCGATG CGTTATGGAA TTGAGAATAT TTATCAAGCT 
ATGACACATT TCCACTGCTT GTCTGCTAAA TACCTATGTC CTGGGGTACC AGCTGTAATG 
AGTACCCTTT TGGCAAACAT AAATGCTTAT TATGCTCATA CGACTGCTTC GTCAGCTGTA 
TCGGCCAGTG ATAGATTTGC TGCCAGCAAT TTTGGGAAAG AAAAATTTGT TAAGTTGCTG 
GACCAGTTGC ATAACTCACT GAGGATCGAT TTGTCAATGT ACAGAAATAA CTTTCCTGCA 
TCGAGTCCAG AAAAGCTAAT GGATCTAAAA TCTACAGTTG ATTTATTGAC CAGCATAACA 
TTTTTCCGCA TGAAGGTACA AGAGCTATCT AGCCCGCCGA GAGCAAGTAC AGTAGTCAAG 
GATTGTGTTA AAGCATGCCT TAGATCCACT TATCAATTCT TATTTGAAAA TTGTTATGAA 
CTGTACAACA GAGAATTTCA GGTTGATCCC AACGAAGCAA AACGTGCACC AGATGATCAT 
GAACCCAAGC TTGATAGCGT CGACTTTTGG CATAAGCTAA TAGCTTTAAT CGTATCGGTA 
ATAGATGAAG ACAAAAACAG TTACGGAACC GTTTTAAATC AATTTCCGCA AGAGCTAAAT 
ATTGGACAGT TATCAGCATC TTCAATGTGG CATCTCTTTG CTGTGGACAT GAAGTACGCG 
CTCGAGGAGC ATGAGCAACA TCGTCTTTGC AAGTCATCGG CTTATATGAA TCTTCACTTT 
CGAGTTAAGT GGCTATATAG TAACTACGTC AAAGAGGTGC CTCCCTACAA AGGAGCGGTT 
CCCGATTACC CAGCATGGTT CGAGCCTTTT GTGATGCAGT GGCTAAATGA AAATGACGAC 
GTGTCATTGG AATACCTTCA TGGCGCATTT AAGAGGGATA AAAAAGACGG GTTTCAAAAG 
AGCAGCGAGC ACGCGCTCTT TTCAAATTCT GTAGTCGACG TTTTTACGCA ATTGACGCAG 
TGTTTCGATG TTGTTAGCAA ACTCGAGTGT CCTGATCCAG AAATTTGGAA ACGTTACATG 
AGACGTTTTG CTAAAACAAT CGTAAAAGTG CTAATAGCCT ATGCGGATAT TGTGAAACTT 
GAGTTTCCAG AGCATATGAA GGATGAAAGA ATTGCTTGCA TACTTATGAA CAACATTCAG 
CAACTAAGGG TGCAGCTAGA AAAAATGTTT GAGTCTATGG GTGGGGATAA GCTAGAGGAG 
GATGCAGCCA ATATTTTGAA AGAACTTCAG CAAAATCTTA ACTCTGCGTT GGACGACTTA 
GCTTCACAAT TCGCTATCAG TTTGGAGCCA CGAATAACTC AATCAGTGCG AGAACTTGGA 
GACATGCTGC TTTCTATTAA AGGAGGCAGT GGAACCCTTG CTGCTGGTAA TCTTGCAGCT 
CAAAGGAACG CGGTCGCCGT TGAGGCCGAT GAAGTTTTAA GGCCGCTTAT GGATTTACTT 
GATGGCTCTT TAACTCTCTA TGCGCAATCA TGTGAAAAAA CCGTGTTGAA GAGGTTACTA 
AAGGAGTTAT GGAAGATCGT AATGCGAATA TTAGAGAAAA CAATTGTTTT ACCACCTATG 
ACTGATAAAA CTATGATGTT TAAACACCTT ACTGACAATG CAAAAAACTT GGCATCGAAT 
GCAAAAATTG AAGACATGGG TCGTTTGTTC AAGTCACATA TGGCAGGAAA GCAGGATGTT 
AAGAGTGCTC TGAGCGGGGT AATGGACATA TCAAAAGAAG TAGAAAAAAA CTTGTCACCC 
AAGCAATGCG CAGTGCTTGA TGTTGCCTTA GATACAATAA AACAATACTT TCACGCGGGT 
GGCAATGGAC TGAAAAAGAC CTTTCTCGAA AAGTCGCCCG AGCTTCAGAG TTTACGTTAC 
GCACTCAGTT TATATACACA AATGACCGAC ACACTAATCA AAACCTTTAT ATCTAGCCAA 
GTTCACGAGG TCGATCTAGA AAACTCAGAG GAAAGTGTTG GGGAAATATC CGTGCAAATA 
GACTTATTTT CCCATCCCGG CACCGGTGAA CATAAAGTAA ACGTGAAAGT TGTTGCTGCT 
AATGACCTTA AATGGCAAAT ACCATCGGGT ATGTTCAGAC CATTTGTTGA CATTAATTTA 
ATAGGACCGC ACCTGCAAGA AAAAAAAAGG AAGTTCGCTA CAAAGTCGAA GTCTAACAAC 
TGGTCGCCTA AATACAACGA ATCTTTCAGC TTCACCATTG GAAATGAGGA GCAGCTGGAC 
TTCTTTGAAT TGCACATTTG CGTTAAGGAC TATTGTTTTG CTCGTGATGA CCGACTTGTG 
GGCGTGGCTG TAATTCCATT AAAAGATATA TCGGAAAAGG GATCGGTCGC ATGCTGGTTA 
CCCTTAATGA GACGAATTGA AATGGACGAA ACGGGCTGGA CCATTTTAAG AATTTTGTCA 
CAGCGAAATA ATGATGAAGT GGCCAAGGAA TTTGTCAAGC TTAAATCGGA AATACGGCAG 
GAGCCAACCA TGGGTACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-13 ( NM_001297788.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001284717.1 Drosophila melanogaster unc-13, transcript variant G (unc-13), mRNA.

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CloneID	OFa22196
Clone ID Related Accession (Same CDS sequence)	NM_001297788.1
Accession Version	NM_001297788.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	unc-13, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004353). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGAC AATGGCCGCT 
ACAAAACGAA ATGCTGGACT CACATCTGCT GTTCCACGTG CAACGCTGAA TGACGAAGAT 
CTGAAAATGC ACGTGTACAA AAAAGCTCTA CAAGCCCTGA TATATCCTAT ATCCTCAACA 
ACGCCCCACA ATTTTTTGTT ATGGACTGCT ACGTCACCAA CGTACTGCTA CGAGTGTGAG 
GGTCTTTTGT GGGGGATAGC ACGACAAGGG GTCCGTTGCA CTGAGTGCGG TGTAAAGTGT 
CATGAAAAAT GTAAGGACCT TCTGAATGCG GACTGCCTGC AGCGTGCTGC CGAAAAAAGC 
TCAAAGCACG GTGCGGAAGA CAAGGCTAAC TCGATTATTA CAGCGATGAA AGATCGTATG 
AAGCAACGTG AACGAGAGAA GCCTGAAATT TTTGAGTTGA TTAGAGCCGT GTTCAGCGTT 
GAGGAGAAGA GTCATGCCGG TCATATGAAG GCTGTGAAGC AAAGCGTCCT GGATGGTACA 
AGCAAGTGGT CGGCCAAAAT AGCAATCACA GTGATTTGTG CTCAAGGCCT GATAGCGAAG 
GATAAAAGCG GTACCAGTGA TCCATATGTG ACGGTGCAAG TTAGCAAGGT GAAAAAACGA 
ACGCGAACAA TGCCGCAGGA ACTTAATCCT GTGTGGAACG AGAAGTTTCA CTTCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284717.1
CDS	3736..12351
Translation	MTHYVRHDYFHNTQNGALSSDTSRISYSQISYETQPSREYFSESYALSNQGPEECSRVSHLNSDTVLTTVDNSNNSYGYDYLECYGANIQCDPEEDSVDNWNENTSVVADQYGLGHNNLNCTSSKLLPKLPNIENGRGSSNACAPQMDVKFNTKGMCIKIDHSYGVCMAKAHDFVGRLSPSDYQNILGNNLNGYAGCAYSSTFLDNAMSSAPLRVLPQSPRCSSYLGRNIIGFNADAAQRDGRGFDTDQTDAMGSESSTYEVYEKMQRPYTSMLPLDYSDYQEGCYNTDNLSTYSDTPPSNNTQLKRQMQRKISLMMAMTTASVIASGEIRVPVHSKQSKKSTEIQTDSIIGNTISTNAAARDLDRCLATESCEVIVDTRDSGSVTSFPSSAVTAITKTRKLPKVLPTPLCKSSRHPITIATDALSSSYTSDPLPEKSHRPKQLPKLPISLPQSNDRASLNSNWATPPAPDALPFNSFDHKSASSPTPTTTITKDTETTSYLVETDFIGARHNALYQYDSKEPNIVFSDKSVEAEHSPTWTPLSPIQSKQSPCPPVALPSNIMQNVSLTCHLPEIEATRSDIEREPESSSIEPILEIEKLADPYSGPGSALFNISEYLKPYTLNKPILSEEKKNHIANAASTSTTTPLNITSDDEFSSYSNKWTSTCVNFQPLDVESSLNISLKVNAGTNQAELLMTPLKSSTPLFISSNGTSDNFNLRKSSPPDSAFTTTVNVNSFETVLVSGSQTASPSPSNLKSPPSIAPLLSYSDYMKQFELPELPQPIMDLSENDTATQSDSFNVINNTLTNADNLNSYNQMDVESKSSLQLPSYSSESFDPCSVPSFSIKNKEYKIVEKLDSLSNVESVESPKTLVSPVNPLNCSKLLPGTESIVSNDVAFDDTFYDSFNVDIKELTAFVDHVAPEDGLYNFPNDKTSVEFSFDKTEDTIDMNQNLSSGECGYYKPSQAQQKASVVASAASSVLDGISKGLKGGLDGVFSGVSSTVDVTQSNPSSKRGFSFNLASKIVPSVGGLLTSTSSTSIKQTGSETNPTLILISPENVSSRNSNYIPTTSPSCTQKNGEENLYSATVHNKSTKSNSYYNEVGEISSTLVRNVCDSYDNSYDEMILTNEMVNIGMLDSESEFGLIENSYSYQVPDNEQIDSVNSYNNKTQNVTNNGIEKANTKNKPVPLHDPPTKKASTVGMFGSILGKAAAAVQSATQAVNQSASSVASVVAQKPTIVPRTNNVLLLSSVCSPNEIKRNSSSVEFDSEYGYQMPDVESLSSHYANTGGDYDNSNMKIHEFGTYADDRPYADYHTNGNQSQFKEEAVIPGEPEVINTNILPIGPQATGKKLPTVNGKSALLIKQMPTEVYDDESDTDELDVSPSTGKVPSYSIYSEQEDYYMDLQQTTPSIQPNGFYEQVNNGYDYREDYFNEEDEYKYLEQQREQEEHNQPKNKKYLKQAKISKIQPPSLDFIDVGQDDDFIYDNYHSEDDSGNYLEGSSSGSVGPIEGSIIKVDSNIEASFASLNKKSDSFTPTNDSLQKHDTVIGESTTKLTRLRTEKMCPDVDEEDENLSDHVSDLTDLSKLISQKKKTLLRGETEEVVGGHMQVLRQTEITARQRWHWAYNKIIMQLNNGGGPGEVGLRTNGHPGDNPFYSNIDSMPDIRPRRKSIPLVSELTMAATKRNAGLTSAVPRATLNDEDLKMHVYKKALQALIYPISSTTPHNFLLWTATSPTYCYECEGLLWGIARQGVRCTECGVKCHEKCKDLLNADCLQRAAEKSSKHGAEDKANSIITAMKDRMKQREREKPEIFELIRAVFSVEEKSHAGHMKAVKQSVLDGTSKWSAKIAITVICAQGLIAKDKSGTSDPYVTVQVSKVKKRTRTMPQELNPVWNEKFHFECHNSSDRIKVRVWDEDNDLKSKLRQKLTRESDDFLGQTIIEVRTLSGEMDVWYNLEKRTDKSAVSGAIRLHISVEIKGEEKVAPYHVQYTCLHENLFHYLCEENTGMVKLPTQKGDDAWKLYFDEIPEEIVDEFSMRYGIENIYQAMTHFHCLSAKYLCPGVPAVMSTLLANINAYYAHTTASSAVSASDRFAASNFGKEKFVKLLDQLHNSLRIDLSMYRNNFPASSPEKLMDLKSTVDLLTSITFFRMKVQELSSPPRASTVVKDCVKACLRSTYQFLFENCYELYNREFQVDPNEAKRAPDDHEPKLDSVDFWHKLIALIVSVIDEDKNSYGTVLNQFPQELNIGQLSASSMWHLFAVDMKYALEEHEQHRLCKSSAYMNLHFRVKWLYSNYVKEVPPYKGAVPDYPAWFEPFVMQWLNENDDVSLEYLHGAFKRDKKDGFQKSSEHALFSNSVVDVFTQLTQCFDVVSKLECPDPEIWKRYMRRFAKTIVKVLIAYADIVKLEFPEHMKDERIACILMNNIQQLRVQLEKMFESMGGDKLEEDAANILKELQQNLNSALDDLASQFAISLEPRITQSVRELGDMLLSIKGGSGTLAAGNLAAQRNAVAVEADEVLRPLMDLLDGSLTLYAQSCEKTVLKRLLKELWKIVMRILEKTIVLPPMTDKTMMFKHLTDNAKNLASNAKIEDMGRLFKSHMAGKQDVKSALSGVMDISKEVEKNLSPKQCAVLDVALDTIKQYFHAGGNGLKKTFLEKSPELQSLRYALSLYTQMTDTLIKTFISSQVHEVDLENSEESVGEISVQIDLFSHPGTGEHKVNVKVVAANDLKWQIPSGMFRPFVDINLIGPHLQEKKRKFATKSKSNNWSPKYNESFSFTIGNEEQLDFFELHICVKDYCFARDDRLVGVAVIPLKDISEKGSVACWLPLMRRIEMDETGWTILRILSQRNNDEVAKEFVKLKSEIRQEPTMGT

Target ORF information:

RefSeq Version	NM_001297788.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster unc-13, transcript variant G (unc-13), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297788.1

ORF Insert Sequence:

ATGACGCACT ACGTGAGGCA TGATTACTTT CATAACACCC AAAACGGTGC CTTATCCTCT 
GACACCAGTC GAATATCTTA CAGTCAAATT TCTTACGAAA CACAGCCTAG CAGAGAATAT 
TTCAGTGAGT CATATGCTTT AAGCAATCAA GGACCAGAAG AATGCAGTCG CGTGAGCCAC 
CTAAACAGTG ATACAGTACT TACAACCGTG GACAACAGCA ACAATAGTTA TGGCTACGAC 
TATTTAGAAT GTTATGGAGC TAATATCCAG TGTGATCCGG AAGAGGATTC TGTTGACAAT 
TGGAACGAAA ACACTTCAGT TGTTGCAGAT CAATATGGGT TAGGACATAA TAATCTAAAT 
TGTACCTCAT CGAAATTATT ACCTAAGCTG CCAAACATTG AAAATGGAAG GGGTTCTTCA 
AACGCATGTG CCCCTCAAAT GGATGTAAAG TTTAACACAA AAGGTATGTG CATAAAAATA 
GACCACAGCT ATGGTGTCTG CATGGCCAAA GCACACGACT TCGTCGGACG TCTTTCGCCT 
AGTGACTACC AGAATATCCT TGGCAATAAT CTCAATGGAT ATGCTGGTTG TGCCTACTCG 
TCGACATTTT TAGACAATGC TATGTCATCG GCACCATTGC GGGTTTTACC GCAATCACCG 
CGGTGTAGCT CTTATTTAGG TCGAAATATA ATTGGATTTA ATGCAGACGC GGCTCAGAGG 
GATGGACGCG GCTTCGACAC AGATCAAACT GATGCGATGG GTTCTGAATC TAGTACATAT 
GAGGTTTATG AAAAAATGCA AAGGCCATAC ACGTCCATGT TGCCGTTAGA TTACAGTGAT 
TACCAAGAAG GTTGTTACAA TACCGACAAT CTTTCAACAT ACAGCGACAC ACCGCCGAGC 
AATAATACAC AACTAAAGAG GCAAATGCAG AGGAAAATTT CGTTAATGAT GGCGATGACT 
ACAGCTTCTG TGATAGCATC TGGTGAGATT AGAGTCCCAG TTCACTCAAA GCAATCTAAG 
AAATCTACTG AAATCCAAAC TGACTCTATA ATAGGAAACA CCATCAGCAC CAATGCCGCT 
GCAAGAGACT TAGACAGATG TTTGGCGACG GAATCTTGTG AAGTAATTGT CGATACACGT 
GACTCTGGTA GCGTCACAAG TTTCCCGTCA TCTGCTGTGA CTGCAATAAC AAAGACTAGA 
AAATTGCCAA AGGTTTTACC AACGCCACTG TGTAAATCAT CTCGTCATCC AATCACCATA 
GCCACCGATG CATTAAGTTC ATCATATACT TCAGATCCTT TACCAGAGAA GAGTCATCGC 
CCAAAGCAAC TGCCAAAGCT GCCTATATCA TTACCCCAGT CCAATGATCG TGCAAGTCTC 
AATTCGAATT GGGCGACCCC TCCTGCCCCA GATGCATTAC CATTTAATTC GTTCGACCAT 
AAATCGGCAT CTTCTCCTAC GCCAACAACA ACAATAACAA AAGATACGGA AACCACCTCA 
TATCTGGTTG AAACAGATTT TATTGGAGCC AGGCATAATG CCCTTTATCA GTATGATAGT 
AAAGAACCAA ATATAGTATT TTCGGACAAG TCTGTCGAAG CAGAACACTC GCCAACGTGG 
ACCCCATTAT CGCCAATACA ATCCAAACAA AGTCCTTGTC CGCCTGTGGC GCTACCAAGC 
AATATTATGC AAAATGTTTC TCTAACATGT CACTTACCTG AAATCGAAGC AACCCGATCC 
GATATTGAGC GAGAACCAGA AAGCTCTTCT ATCGAACCAA TTCTAGAAAT TGAAAAATTA 
GCCGATCCAT ATAGCGGGCC CGGATCAGCC TTATTTAACA TATCTGAGTA TCTGAAGCCT 
TATACATTAA ATAAACCTAT TTTGTCCGAA GAAAAGAAAA ACCATATCGC CAATGCTGCC 
AGTACGTCAA CCACAACGCC ATTGAATATA ACATCAGATG ACGAATTCTC TTCATATTCT 
AACAAGTGGA CGAGTACTTG CGTTAATTTT CAACCATTAG ACGTTGAATC AAGCTTAAAT 
ATTTCACTTA AAGTCAACGC TGGCACGAAT CAGGCCGAAT TATTAATGAC ACCATTAAAA 
AGTTCCACTC CTCTTTTCAT TTCTTCTAAT GGCACATCCG ATAATTTTAA TTTGAGGAAA 
AGCTCTCCTC CCGACAGCGC GTTCACCACC ACGGTTAACG TCAACAGTTT TGAAACGGTA 
CTAGTATCTG GAAGCCAAAC TGCCTCACCG TCGCCGTCAA ACTTAAAGTC ACCACCATCA 
ATAGCTCCAT TATTATCCTA CTCGGATTAT ATGAAACAAT TTGAGTTACC AGAATTGCCG 
CAACCCATTA TGGATCTTTC AGAAAATGAT ACTGCTACTC AAAGCGATTC CTTTAATGTA 
ATCAATAATA CTCTTACGAA CGCTGACAAT TTAAATTCCT ACAACCAGAT GGATGTAGAA 
AGCAAAAGTA GTCTACAGTT ACCTTCTTAT TCCAGTGAAT CATTTGATCC GTGTAGTGTG 
CCTAGTTTTT CAATTAAGAA CAAAGAATAT AAAATTGTCG AAAAGCTGGA CAGTCTATCA 
AATGTTGAAA GCGTAGAATC ACCGAAAACC TTAGTAAGTC CAGTAAACCC ATTGAATTGC 
TCAAAATTAT TGCCAGGGAC GGAATCAATA GTGAGTAATG ATGTAGCGTT CGATGATACA 
TTTTACGATA GCTTTAATGT TGACATAAAA GAACTGACCG CATTTGTTGA CCATGTTGCC 
CCAGAGGACG GTTTATACAA TTTCCCAAAC GATAAGACTT CAGTTGAATT CAGTTTTGAT 
AAGACCGAGG ATACAATAGA CATGAATCAG AACTTAAGCT CAGGAGAATG TGGTTATTAT 
AAGCCATCTC AGGCACAACA AAAAGCTAGT GTAGTAGCAT CCGCAGCAAG TAGTGTTCTT 
GACGGAATTT CAAAGGGCTT AAAAGGGGGG CTGGACGGCG TTTTTAGTGG TGTCAGTTCA 
ACAGTTGATG TCACTCAATC AAATCCCAGT TCCAAAAGAG GTTTTAGCTT TAACTTAGCA 
TCAAAGATTG TGCCAAGTGT CGGTGGATTG CTAACTAGTA CAAGTTCCAC CTCTATAAAA 
CAAACAGGAT CAGAAACTAA TCCTACCCTA ATATTGATTT CACCTGAAAA TGTTTCTAGT 
CGTAACAGTA ATTATATACC TACGACATCT CCATCTTGTA CTCAAAAAAA TGGCGAGGAA 
AATTTATATT CGGCTACAGT CCACAATAAG TCAACGAAAT CGAATTCATA CTATAATGAA 
GTGGGAGAAA TTAGTTCGAC CTTAGTCAGA AATGTTTGTG ACAGTTACGA TAATAGCTAT 
GATGAGATGA TTTTAACCAA CGAAATGGTA AATATAGGGA TGCTCGACAG TGAATCTGAG 
TTTGGCTTAA TAGAAAATTC ATATTCGTAT CAGGTACCAG ACAATGAGCA AATCGACAGT 
GTTAACTCGT ATAATAATAA GACCCAAAAT GTAACAAATA ATGGCATAGA AAAGGCAAAT 
ACAAAAAATA AACCAGTGCC TCTTCATGAC CCTCCTACGA AAAAAGCAAG TACTGTTGGC 
ATGTTCGGAT CAATTTTGGG AAAAGCTGCT GCAGCAGTGC AATCAGCAAC GCAAGCAGTC 
AATCAAAGTG CGTCGTCAGT GGCATCAGTC GTTGCACAAA AGCCAACAAT CGTACCGAGA 
ACGAACAACG TGCTTTTGTT GTCTAGCGTA TGCTCACCTA ACGAGATTAA AAGAAATTCA 
AGTTCAGTAG AATTTGATTC AGAATATGGA TATCAAATGC CCGATGTAGA GTCTCTATCA 
AGCCATTACG CAAATACTGG CGGTGATTAT GATAATAGTA ATATGAAAAT ACATGAATTT 
GGTACATATG CAGATGATAG ACCATACGCA GATTATCATA CGAATGGCAA TCAAAGCCAA 
TTTAAAGAAG AGGCGGTGAT TCCTGGGGAA CCTGAAGTAA TTAATACAAA CATACTCCCG 
ATTGGCCCGC AGGCCACCGG CAAAAAACTT CCTACCGTCA ATGGTAAATC GGCGTTACTT 
ATTAAACAGA TGCCGACGGA GGTATATGAT GATGAATCAG ATACAGATGA ATTAGATGTC 
AGTCCATCAA CCGGAAAAGT ACCTAGCTAT AGCATTTATA GTGAGCAGGA AGATTACTAC 
ATGGATCTTC AGCAAACGAC ACCGTCAATT CAGCCAAACG GTTTTTATGA ACAAGTCAAT 
AATGGTTATG ACTACCGAGA GGATTACTTT AACGAGGAGG ATGAATATAA GTATCTCGAG 
CAGCAGAGGG AGCAAGAGGA ACATAATCAG CCGAAGAACA AGAAATATCT GAAGCAGGCC 
AAAATATCAA AAATACAACC ACCATCATTA GATTTCATTG ACGTAGGGCA AGACGATGAC 
TTTATATACG ACAACTATCA CAGCGAAGAC GATAGCGGAA ATTACCTAGA GGGAAGCTCC 
AGTGGAAGCG TTGGTCCTAT TGAAGGGTCA ATCATAAAGG TGGACTCGAA TATAGAAGCT 
TCGTTTGCTT CGCTCAACAA GAAGAGTGAC TCTTTCACAC CGACAAACGA TTCCCTTCAA 
AAGCATGACA CTGTTATTGG TGAATCAACA ACCAAACTTA CGCGCCTTAG GACAGAAAAA 
ATGTGCCCAG ACGTAGACGA AGAAGATGAA AACCTAAGCG ATCACGTTTC CGATCTTACT 
GACTTAAGTA AACTAATCTC ACAAAAAAAA AAAACGCTCT TGAGAGGAGA AACTGAAGAA 
GTCGTAGGAG GACACATGCA GGTGTTACGC CAAACGGAAA TCACAGCTCG GCAACGATGG 
CATTGGGCTT ACAATAAAAT AATCATGCAG CTTAATAATG GAGGTGGTCC TGGAGAAGTG 
GGATTACGTA CAAATGGTCA TCCCGGTGAC AATCCATTCT ATAGCAATAT TGACAGTATG 
CCGGACATAA GGCCTCGAAG AAAATCCATT CCTCTTGTGT CTGAGCTGAC AATGGCCGCT 
ACAAAACGAA ATGCTGGACT CACATCTGCT GTTCCACGTG CAACGCTGAA TGACGAAGAT 
CTGAAAATGC ACGTGTACAA AAAAGCTCTA CAAGCCCTGA TATATCCTAT ATCCTCAACA 
ACGCCCCACA ATTTTTTGTT ATGGACTGCT ACGTCACCAA CGTACTGCTA CGAGTGTGAG 
GGTCTTTTGT GGGGGATAGC ACGACAAGGG GTCCGTTGCA CTGAGTGCGG TGTAAAGTGT 
CATGAAAAAT GTAAGGACCT TCTGAATGCG GACTGCCTGC AGCGTGCTGC CGAAAAAAGC 
TCAAAGCACG GTGCGGAAGA CAAGGCTAAC TCGATTATTA CAGCGATGAA AGATCGTATG 
AAGCAACGTG AACGAGAGAA GCCTGAAATT TTTGAGTTGA TTAGAGCCGT GTTCAGCGTT 
GAGGAGAAGA GTCATGCCGG TCATATGAAG GCTGTGAAGC AAAGCGTCCT GGATGGTACA 
AGCAAGTGGT CGGCCAAAAT AGCAATCACA GTGATTTGTG CTCAAGGCCT GATAGCGAAG 
GATAAAAGCG GTACCAGTGA TCCATATGTG ACGGTGCAAG TTAGCAAGGT GAAAAAACGA 
ACGCGAACAA TGCCGCAGGA ACTTAATCCT GTGTGGAACG AGAAGTTTCA CTTCGAGTGC 
CACAACTCCT CCGACCGCAT TAAGGTTCGT GTTTGGGACG AAGACAATGA CCTAAAGTCG 
AAACTGCGCC AGAAGTTAAC TCGAGAGTCG GACGACTTTT TGGGACAAAC AATTATCGAG 
GTTCGTACTC TTTCCGGTGA AATGGATGTA TGGTACAACC TGGAAAAGCG CACAGATAAA 
TCGGCCGTAT CAGGCGCGAT ACGGCTTCAC ATATCTGTTG AAATAAAGGG CGAGGAAAAA 
GTAGCTCCTT ACCACGTACA ATATACCTGT TTACACGAGA ACCTCTTCCA TTATCTTTGC 
GAAGAAAATA CCGGAATGGT CAAGTTGCCA ACTCAAAAAG GGGACGATGC TTGGAAGTTG 
TATTTTGATG AAATACCCGA AGAGATTGTC GACGAGTTTT CGATGCGTTA TGGAATTGAG 
AATATTTATC AAGCTATGAC ACATTTCCAC TGCTTGTCTG CTAAATACCT ATGTCCTGGG 
GTACCAGCTG TAATGAGTAC CCTTTTGGCA AACATAAATG CTTATTATGC TCATACGACT 
GCTTCGTCAG CTGTATCGGC CAGTGATAGA TTTGCTGCCA GCAATTTTGG GAAAGAAAAA 
TTTGTTAAGT TGCTGGACCA GTTGCATAAC TCACTGAGGA TCGATTTGTC AATGTACAGA 
AATAACTTTC CTGCATCGAG TCCAGAAAAG CTAATGGATC TAAAATCTAC AGTTGATTTA 
TTGACCAGCA TAACATTTTT CCGCATGAAG GTACAAGAGC TATCTAGCCC GCCGAGAGCA 
AGTACAGTAG TCAAGGATTG TGTTAAAGCA TGCCTTAGAT CCACTTATCA ATTCTTATTT 
GAAAATTGTT ATGAACTGTA CAACAGAGAA TTTCAGGTTG ATCCCAACGA AGCAAAACGT 
GCACCAGATG ATCATGAACC CAAGCTTGAT AGCGTCGACT TTTGGCATAA GCTAATAGCT 
TTAATCGTAT CGGTAATAGA TGAAGACAAA AACAGTTACG GAACCGTTTT AAATCAATTT 
CCGCAAGAGC TAAATATTGG ACAGTTATCA GCATCTTCAA TGTGGCATCT CTTTGCTGTG 
GACATGAAGT ACGCGCTCGA GGAGCATGAG CAACATCGTC TTTGCAAGTC ATCGGCTTAT 
ATGAATCTTC ACTTTCGAGT TAAGTGGCTA TATAGTAACT ACGTCAAAGA GGTGCCTCCC 
TACAAAGGAG CGGTTCCCGA TTACCCAGCA TGGTTCGAGC CTTTTGTGAT GCAGTGGCTA 
AATGAAAATG ACGACGTGTC ATTGGAATAC CTTCATGGCG CATTTAAGAG GGATAAAAAA 
GACGGGTTTC AAAAGAGCAG CGAGCACGCG CTCTTTTCAA ATTCTGTAGT CGACGTTTTT 
ACGCAATTGA CGCAGTGTTT CGATGTTGTT AGCAAACTCG AGTGTCCTGA TCCAGAAATT 
TGGAAACGTT ACATGAGACG TTTTGCTAAA ACAATCGTAA AAGTGCTAAT AGCCTATGCG 
GATATTGTGA AACTTGAGTT TCCAGAGCAT ATGAAGGATG AAAGAATTGC TTGCATACTT 
ATGAACAACA TTCAGCAACT AAGGGTGCAG CTAGAAAAAA TGTTTGAGTC TATGGGTGGG 
GATAAGCTAG AGGAGGATGC AGCCAATATT TTGAAAGAAC TTCAGCAAAA TCTTAACTCT 
GCGTTGGACG ACTTAGCTTC ACAATTCGCT ATCAGTTTGG AGCCACGAAT AACTCAATCA 
GTGCGAGAAC TTGGAGACAT GCTGCTTTCT ATTAAAGGAG GCAGTGGAAC CCTTGCTGCT 
GGTAATCTTG CAGCTCAAAG GAACGCGGTC GCCGTTGAGG CCGATGAAGT TTTAAGGCCG 
CTTATGGATT TACTTGATGG CTCTTTAACT CTCTATGCGC AATCATGTGA AAAAACCGTG 
TTGAAGAGGT TACTAAAGGA GTTATGGAAG ATCGTAATGC GAATATTAGA GAAAACAATT 
GTTTTACCAC CTATGACTGA TAAAACTATG ATGTTTAAAC ACCTTACTGA CAATGCAAAA 
AACTTGGCAT CGAATGCAAA AATTGAAGAC ATGGGTCGTT TGTTCAAGTC ACATATGGCA 
GGAAAGCAGG ATGTTAAGAG TGCTCTGAGC GGGGTAATGG ACATATCAAA AGAAGTAGAA 
AAAAACTTGT CACCCAAGCA ATGCGCAGTG CTTGATGTTG CCTTAGATAC AATAAAACAA 
TACTTTCACG CGGGTGGCAA TGGACTGAAA AAGACCTTTC TCGAAAAGTC GCCCGAGCTT 
CAGAGTTTAC GTTACGCACT CAGTTTATAT ACACAAATGA CCGACACACT AATCAAAACC 
TTTATATCTA GCCAAGTTCA CGAGGTCGAT CTAGAAAACT CAGAGGAAAG TGTTGGGGAA 
ATATCCGTGC AAATAGACTT ATTTTCCCAT CCCGGCACCG GTGAACATAA AGTAAACGTG 
AAAGTTGTTG CTGCTAATGA CCTTAAATGG CAAATACCAT CGGGTATGTT CAGACCATTT 
GTTGACATTA ATTTAATAGG ACCGCACCTG CAAGAAAAAA AAAGGAAGTT CGCTACAAAG 
TCGAAGTCTA ACAACTGGTC GCCTAAATAC AACGAATCTT TCAGCTTCAC CATTGGAAAT 
GAGGAGCAGC TGGACTTCTT TGAATTGCAC ATTTGCGTTA AGGACTATTG TTTTGCTCGT 
GATGACCGAC TTGTGGGCGT GGCTGTAATT CCATTAAAAG ATATATCGGA AAAGGGATCG 
GTCGCATGCT GGTTACCCTT AATGAGACGA ATTGAAATGG ACGAAACGGG CTGGACCATT 
TTAAGAATTT TGTCACAGCG AAATAATGAT GAAGTGGCCA AGGAATTTGT CAAGCTTAAA 
TCGGAAATAC GGCAGGAGCC AACCATGGGT ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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unc-13 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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